AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0264740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 89 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACACTGGGGCATATATTT+TGG 0.168374 2_2:-64682310 None:intergenic
AACCCTAACCTTTGAAGATT+TGG 0.216041 2_2:-64680244 None:intergenic
GCGACGTGGGGAATGGTTAT+TGG 0.245644 2_2:-64680072 None:intergenic
CGGTTCTGCAGAGATGGTTT+TGG 0.250126 2_2:-64681873 None:intergenic
CGACGTGGGGAATGGTTATT+GGG 0.261317 2_2:-64680071 None:intergenic
GTACAATCTACTCATGTCTA+TGG 0.321807 2_2:-64682472 None:intergenic
ACCCTAACCTTTGAAGATTT+GGG 0.323946 2_2:-64680243 None:intergenic
GAGACTGCATTTCATTTGAT+TGG 0.329337 2_2:+64682287 Msa0264740:three_prime_UTR
TGTAATAGTACATGTCAAAA+TGG 0.338938 2_2:+64682417 Msa0264740:three_prime_UTR
GTACTATTACAGAGATAGAT+AGG 0.339194 2_2:-64682405 None:intergenic
CACTGATTGGGGTGCGACGT+GGG 0.366614 2_2:-64680085 None:intergenic
GTTTCAGCATCATGTCTTTG+TGG 0.367335 2_2:-64681730 None:intergenic
TTAAATCTCTGCTAGTTTAG+TGG 0.376282 2_2:+64682342 Msa0264740:three_prime_UTR
ACGAACGCTCTTCACTGATT+GGG 0.397865 2_2:-64680097 None:intergenic
CATCATGTCTTTGTGGCACT+TGG 0.399820 2_2:-64681723 None:intergenic
TTCCGGCTCAGAGGAGAGTT+TGG 0.400683 2_2:+64681904 Msa0264740:three_prime_UTR
CCAGCAGTGCGGTAATCAAA+TGG 0.411400 2_2:-64682055 None:intergenic
GCAGAACCGTTAGTTGCTTC+CGG 0.413723 2_2:+64681887 Msa0264740:three_prime_UTR
AACGAACGCTCTTCACTGAT+TGG 0.419839 2_2:-64680098 None:intergenic
AATAAGCGGGTTGGTTTGAC+AGG 0.421004 2_2:+64681968 Msa0264740:three_prime_UTR
CAAAGACATGATGCTGAAAC+AGG 0.424095 2_2:+64681733 Msa0264740:three_prime_UTR
CATGTATTAAAATTTAATAG+TGG 0.424336 2_2:+64682577 Msa0264740:three_prime_UTR
GGGAATGGTTATTGGGGTTG+AGG 0.428999 2_2:-64680064 None:intergenic
CACTTGCAGCATCTTCCATT+GGG 0.430249 2_2:+64681765 Msa0264740:three_prime_UTR
AGCTTGACAAGATCTAGATT+TGG 0.434195 2_2:+64682122 Msa0264740:three_prime_UTR
ATGTATTAAAATTTAATAGT+GGG 0.442884 2_2:+64682578 Msa0264740:three_prime_UTR
TTGCAGTTGCTGCACCGCTT+AGG 0.445309 2_2:-64681947 None:intergenic
TCCATTGGGAACATCATGAA+TGG 0.454077 2_2:+64681779 Msa0264740:three_prime_UTR
TTGGTGGTGTCTGCATTATA+AGG 0.456260 2_2:+64682368 Msa0264740:three_prime_UTR
GGGATCGAAGAGGGAGAAAG+AGG 0.460650 2_2:-64680223 None:intergenic
CCATTTGATTACCGCACTGC+TGG 0.477078 2_2:+64682055 Msa0264740:three_prime_UTR
ATGCACAACATAGGACCCTC+TGG 0.486214 2_2:-64681653 None:intergenic
ACACTTGCAGCATCTTCCAT+TGG 0.488087 2_2:+64681764 Msa0264740:three_prime_UTR
TCTGCTAGTTTAGTGGAAGT+TGG 0.488824 2_2:+64682349 Msa0264740:three_prime_UTR
GGCTATCATTGAATTTCTCT+AGG 0.502773 2_2:+64682516 Msa0264740:three_prime_UTR
CTTCACATCCAGTCTGATCA+TGG 0.503432 2_2:-64681625 None:intergenic
CAGCAGTGCGGTAATCAAAT+GGG 0.506125 2_2:-64682054 None:intergenic
CACCGCTTAGGACCATCCTT+CGG 0.507315 2_2:-64681935 None:intergenic
AAGAATGCGTTGAATGTGCT+TGG 0.508004 2_2:+64682541 Msa0264740:three_prime_UTR
AAAACACCATTAAAATATGC+AGG 0.511433 2_2:-64682210 None:intergenic
TTTAAAAGACTTCAAAACAC+TGG 0.514164 2_2:-64682324 None:intergenic
TCCACGCTGGCAGTGATAGC+AGG 0.514469 2_2:-64681827 None:intergenic
CTCCAAACTCTCCTCTGAGC+CGG 0.515481 2_2:-64681906 None:intergenic
AACTAACGGTTCTGCAGAGA+TGG 0.516940 2_2:-64681879 None:intergenic
CTTCGATCCCAAATCTTCAA+AGG 0.519999 2_2:+64680236 Msa0264740:CDS
TTGGGGTGCGACGTGGGGAA+TGG 0.520040 2_2:-64680079 None:intergenic
TAGATTGTACTATATTATTG+TGG 0.520791 2_2:+64682485 Msa0264740:three_prime_UTR
AGGTTCAATTACACTGCTGC+TGG 0.521288 2_2:-64681807 None:intergenic
TCACTGATTGGGGTGCGACG+TGG 0.523731 2_2:-64680086 None:intergenic
GTGCAGCAACTGCAATAAGC+GGG 0.530850 2_2:+64681955 Msa0264740:three_prime_UTR
TGAAGATTTGGGATCGAAGA+GGG 0.532574 2_2:-64680232 None:intergenic
GCTAGTTTAGTGGAAGTTGG+TGG 0.537857 2_2:+64682352 Msa0264740:three_prime_UTR
TTGAAGATTTGGGATCGAAG+AGG 0.538680 2_2:-64680233 None:intergenic
CCATTGGGAACATCATGAAT+GGG 0.543973 2_2:+64681780 Msa0264740:three_prime_UTR
TTAAAAGACTTCAAAACACT+GGG 0.551489 2_2:-64682323 None:intergenic
TGTATTAAAATTTAATAGTG+GGG 0.557692 2_2:+64682579 Msa0264740:three_prime_UTR
AAAAGCAAACCCTGTTGTGA+AGG 0.563268 2_2:+64682093 Msa0264740:three_prime_UTR
CGAACGCTCTTCACTGATTG+GGG 0.566023 2_2:-64680096 None:intergenic
GCACTTGGAACACATGTTCA+TGG 0.566316 2_2:-64681708 None:intergenic
ACTGATTGGGGTGCGACGTG+GGG 0.570296 2_2:-64680084 None:intergenic
TTGGAGAAGAAGCCGAAGGA+TGG 0.571839 2_2:+64681923 Msa0264740:three_prime_UTR
AGAATGCGTTGAATGTGCTT+GGG 0.577133 2_2:+64682542 Msa0264740:three_prime_UTR
CCCATTCATGATGTTCCCAA+TGG 0.580215 2_2:-64681780 None:intergenic
TGCACAACATAGGACCCTCT+GGG 0.580560 2_2:-64681652 None:intergenic
AGCGATGTACAGCACAGTAA+AGG 0.582881 2_2:-64682012 None:intergenic
AGCAACTGCAATAAGCGGGT+TGG 0.585228 2_2:+64681959 Msa0264740:three_prime_UTR
ACAGGATTTAATTGTCGATG+TGG 0.589267 2_2:+64681986 Msa0264740:three_prime_UTR
ATTAAAATATGCAGGAAACT+CGG 0.591156 2_2:-64682202 None:intergenic
CATGATGCTGAAACAGGAGC+AGG 0.593551 2_2:+64681739 Msa0264740:three_prime_UTR
AATCAGTGAAGAGCGTTCGT+TGG 0.599174 2_2:+64680100 Msa0264740:CDS
CAGTTGTTGATGCACAACAT+AGG 0.601548 2_2:-64681662 None:intergenic
GGAGATCTCAGTCGATCAAG+TGG 0.604936 2_2:+64681847 Msa0264740:three_prime_UTR
AAGATGGACCATGATCAGAC+TGG 0.612119 2_2:+64681617 Msa0264740:three_prime_UTR
GGATGTGAAGCTGCCCCAGA+GGG 0.615247 2_2:+64681638 Msa0264740:three_prime_UTR
TACTATTACAGAGATAGATA+GGG 0.625786 2_2:-64682404 None:intergenic
TCTGAGCCGGAAGCAACTAA+CGG 0.631746 2_2:-64681893 None:intergenic
GAGTTTGGAGAAGAAGCCGA+AGG 0.631849 2_2:+64681919 Msa0264740:three_prime_UTR
GGTGCAGCAACTGCAATAAG+CGG 0.632084 2_2:+64681954 Msa0264740:three_prime_UTR
AATGGAAGATGCTGCAAGTG+TGG 0.642024 2_2:-64681762 None:intergenic
GTTAGTTGCTTCCGGCTCAG+AGG 0.650978 2_2:+64681895 Msa0264740:three_prime_UTR
ATCGACTGAGATCTCCACGC+TGG 0.665451 2_2:-64681840 None:intergenic
TGGATGTGAAGCTGCCCCAG+AGG 0.681846 2_2:+64681637 Msa0264740:three_prime_UTR
ACCTGCTATCACTGCCAGCG+TGG 0.686751 2_2:+64681826 Msa0264740:three_prime_UTR
AGCCGAAGGATGGTCCTAAG+CGG 0.700565 2_2:+64681933 Msa0264740:three_prime_UTR
GACGTGGGGAATGGTTATTG+GGG 0.715283 2_2:-64680070 None:intergenic
ATGTTGTGCATCAACAACTG+TGG 0.726175 2_2:+64681665 Msa0264740:three_prime_UTR
GCACAACATAGGACCCTCTG+GGG 0.736272 2_2:-64681651 None:intergenic
TTGCATCACGTCCAGCAGTG+CGG 0.742537 2_2:-64682066 None:intergenic
TAAAAGACTTCAAAACACTG+GGG 0.756956 2_2:-64682322 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAATTTATTTATAT+TGG + chr2_2:64680642-64680661 Msa0264740:intron 0.0%
!! AATTAATTACAAAATCAATA+AGG - chr2_2:64680629-64680648 None:intergenic 10.0%
!!! TATTATATTTTTATATGTGA+TGG + chr2_2:64682233-64682252 Msa0264740:three_prime_UTR 10.0%
!!! ATTATATTTTTATATGTGAT+GGG + chr2_2:64682234-64682253 Msa0264740:three_prime_UTR 10.0%
!! ATGTATTAAAATTTAATAGT+GGG + chr2_2:64682578-64682597 Msa0264740:three_prime_UTR 10.0%
!! AAACACACAAAAAAAAAAAA+AGG + chr2_2:64680153-64680172 Msa0264740:CDS 15.0%
!!! TCTATGTTTTCATTTTTATT+TGG + chr2_2:64680284-64680303 Msa0264740:intron 15.0%
!!! TTGTACAGTTTTTATTTTTA+AGG + chr2_2:64680495-64680514 Msa0264740:intron 15.0%
!! ATTGAAAATTGAATGTTATT+TGG + chr2_2:64680716-64680735 Msa0264740:intron 15.0%
!!! GTTTTGTTTTGTTTTTAATA+AGG + chr2_2:64680884-64680903 Msa0264740:intron 15.0%
!! ATAAATTTATGAAAAGTTCA+CGG + chr2_2:64681191-64681210 Msa0264740:intron 15.0%
!! ATTATGCTATATAAATTAAG+TGG + chr2_2:64681476-64681495 Msa0264740:intron 15.0%
!!! TTATATTTTTATATGTGATG+GGG + chr2_2:64682235-64682254 Msa0264740:three_prime_UTR 15.0%
!!! TATATTATTGTGGTTTTTTA+TGG + chr2_2:64682495-64682514 Msa0264740:three_prime_UTR 15.0%
!! CATGTATTAAAATTTAATAG+TGG + chr2_2:64682577-64682596 Msa0264740:three_prime_UTR 15.0%
!! TGTATTAAAATTTAATAGTG+GGG + chr2_2:64682579-64682598 Msa0264740:three_prime_UTR 15.0%
!! TGAAAACATAGAAGATAATT+AGG - chr2_2:64680276-64680295 None:intergenic 20.0%
!!! TTTTTTGAATTTGTGAATAC+AGG + chr2_2:64680376-64680395 Msa0264740:intron 20.0%
!!! TTTTATATGATGATATGAGA+AGG + chr2_2:64680935-64680954 Msa0264740:intron 20.0%
!!! TATTCATCAAATAAAGCATT+GGG - chr2_2:64681104-64681123 None:intergenic 20.0%
!! ATATTCATCAAATAAAGCAT+TGG - chr2_2:64681105-64681124 None:intergenic 20.0%
!!! TGTATAAGGTTGATATATTT+TGG + chr2_2:64681150-64681169 Msa0264740:intron 20.0%
!! CAATTTATGTTTATATGGTT+TGG + chr2_2:64681560-64681579 Msa0264740:intron 20.0%
!! GTTTGGTATTTAAATTTCTT+TGG + chr2_2:64681577-64681596 Msa0264740:intron 20.0%
!!! TATATTTTTATATGTGATGG+GGG + chr2_2:64682236-64682255 Msa0264740:three_prime_UTR 20.0%
!! TAGATTGTACTATATTATTG+TGG + chr2_2:64682485-64682504 Msa0264740:three_prime_UTR 20.0%
! CATAGAAGATAATTAGGAAA+TGG - chr2_2:64680270-64680289 None:intergenic 25.0%
!!! TTTTAAGAATTTTAGTTCCG+AGG + chr2_2:64680546-64680565 Msa0264740:intron 25.0%
!!! GTTTTTGAAAATTTGAGTTG+TGG + chr2_2:64680664-64680683 Msa0264740:intron 25.0%
! ACAAGATGACAAAATTAGTT+AGG - chr2_2:64681236-64681255 None:intergenic 25.0%
!! TTTGTCATCTTGTTTATTTG+AGG + chr2_2:64681243-64681262 Msa0264740:intron 25.0%
!! AAATAACTCATAAAAGCACA+CGG - chr2_2:64681314-64681333 None:intergenic 25.0%
!! GTCTATTGATTATCTCTTTA+AGG - chr2_2:64681363-64681382 None:intergenic 25.0%
!! TATTTAAATTTCTTTGGTGC+AGG + chr2_2:64681583-64681602 Msa0264740:intron 25.0%
! ATTAAAATATGCAGGAAACT+CGG - chr2_2:64682205-64682224 None:intergenic 25.0%
! AAAACACCATTAAAATATGC+AGG - chr2_2:64682213-64682232 None:intergenic 25.0%
! TTAAAAGACTTCAAAACACT+GGG - chr2_2:64682326-64682345 None:intergenic 25.0%
! TTTAAAAGACTTCAAAACAC+TGG - chr2_2:64682327-64682346 None:intergenic 25.0%
! TACTATTACAGAGATAGATA+GGG - chr2_2:64682407-64682426 None:intergenic 25.0%
! TGTAATAGTACATGTCAAAA+TGG + chr2_2:64682417-64682436 Msa0264740:three_prime_UTR 25.0%
TTATACATGCAGATTTACGT+AGG + chr2_2:64680595-64680614 Msa0264740:intron 30.0%
TATACATGCAGATTTACGTA+GGG + chr2_2:64680596-64680615 Msa0264740:intron 30.0%
CTTATACAATTAGTGTGTGT+TGG - chr2_2:64681138-64681157 None:intergenic 30.0%
CAACACACACTAATTGTATA+AGG + chr2_2:64681136-64681155 Msa0264740:intron 30.0%
! ATCTCTTTAAGGATGTCTAT+AGG - chr2_2:64681352-64681371 None:intergenic 30.0%
AATACATGAGCTCTTAAACA+AGG - chr2_2:64681402-64681421 None:intergenic 30.0%
AAATGAGCCAAATGAAACTT+GGG - chr2_2:64681428-64681447 None:intergenic 30.0%
TAAATGAGCCAAATGAAACT+TGG - chr2_2:64681429-64681448 None:intergenic 30.0%
AAGTGGTTAATATGCATAGT+TGG + chr2_2:64681493-64681512 Msa0264740:intron 30.0%
AACGCCATTTATTATTAGCT+AGG + chr2_2:64681533-64681552 Msa0264740:intron 30.0%
GCGAGCAATTTATGTTTATA+TGG + chr2_2:64681555-64681574 Msa0264740:intron 30.0%
! ATCAACAACTGTGGATTTTT+CGG + chr2_2:64681674-64681693 Msa0264740:three_prime_UTR 30.0%
!! GAGTTTCCTGCATATTTTAA+TGG + chr2_2:64682204-64682223 Msa0264740:three_prime_UTR 30.0%
TAAAAGACTTCAAAACACTG+GGG - chr2_2:64682325-64682344 None:intergenic 30.0%
! TTAAATCTCTGCTAGTTTAG+TGG + chr2_2:64682342-64682361 Msa0264740:three_prime_UTR 30.0%
GTACTATTACAGAGATAGAT+AGG - chr2_2:64682408-64682427 None:intergenic 30.0%
ATCCCAAATCTTCAAAGGTT+AGG + chr2_2:64680241-64680260 Msa0264740:intron 35.0%
TCCCAAATCTTCAAAGGTTA+GGG + chr2_2:64680242-64680261 Msa0264740:intron 35.0%
! ACCCTAACCTTTGAAGATTT+GGG - chr2_2:64680246-64680265 None:intergenic 35.0%
! AACCCTAACCTTTGAAGATT+TGG - chr2_2:64680247-64680266 None:intergenic 35.0%
ACGATTCTGATCTTGTAGAT+CGG + chr2_2:64680412-64680431 Msa0264740:intron 35.0%
!!! GCTTGAAGCTTTCGTTTTTT+TGG + chr2_2:64680434-64680453 Msa0264740:intron 35.0%
!!! CTTTCGTTTTTTTGGCTTCA+AGG + chr2_2:64680442-64680461 Msa0264740:intron 35.0%
ATACATGCAGATTTACGTAG+GGG + chr2_2:64680597-64680616 Msa0264740:intron 35.0%
TCTGTTTCATGTTAGTCAAC+TGG + chr2_2:64680788-64680807 Msa0264740:intron 35.0%
TGAGAAGGTTTGTGATCTAA+TGG + chr2_2:64680950-64680969 Msa0264740:intron 35.0%
!! ACTTGTTATCGTCGTCTATT+TGG - chr2_2:64680981-64681000 None:intergenic 35.0%
! GTCATCTTGTTTATTTGAGG+TGG + chr2_2:64681246-64681265 Msa0264740:intron 35.0%
! TTTATTTGAGGTGGAGTAGT+AGG + chr2_2:64681255-64681274 Msa0264740:intron 35.0%
CTTAAACAAGGAAAGAACAG+AGG - chr2_2:64681390-64681409 None:intergenic 35.0%
TATTGACCCCAAGTTTCATT+TGG + chr2_2:64681418-64681437 Msa0264740:intron 35.0%
AATGAGCCAAATGAAACTTG+GGG - chr2_2:64681427-64681446 None:intergenic 35.0%
! GCATAATCAGCACTATTTTC+AGG - chr2_2:64681463-64681482 None:intergenic 35.0%
CTCGCCTAGCTAATAATAAA+TGG - chr2_2:64681540-64681559 None:intergenic 35.0%
GCAGGAAAGTTGAAAAAAGA+TGG + chr2_2:64681601-64681620 Msa0264740:intron 35.0%
ACAGGATTTAATTGTCGATG+TGG + chr2_2:64681986-64682005 Msa0264740:three_prime_UTR 35.0%
AGCTTGACAAGATCTAGATT+TGG + chr2_2:64682122-64682141 Msa0264740:three_prime_UTR 35.0%
GAGACTGCATTTCATTTGAT+TGG + chr2_2:64682287-64682306 Msa0264740:three_prime_UTR 35.0%
! AAACACTGGGGCATATATTT+TGG - chr2_2:64682313-64682332 None:intergenic 35.0%
GTACAATCTACTCATGTCTA+TGG - chr2_2:64682475-64682494 None:intergenic 35.0%
GGCTATCATTGAATTTCTCT+AGG + chr2_2:64682516-64682535 Msa0264740:three_prime_UTR 35.0%
! TGAAGATTTGGGATCGAAGA+GGG - chr2_2:64680235-64680254 None:intergenic 40.0%
! TTGAAGATTTGGGATCGAAG+AGG - chr2_2:64680236-64680255 None:intergenic 40.0%
CTTCGATCCCAAATCTTCAA+AGG + chr2_2:64680236-64680255 Msa0264740:CDS 40.0%
!!! CGTTTTTTTGGCTTCAAGGT+TGG + chr2_2:64680446-64680465 Msa0264740:intron 40.0%
!!! GAATTTTAGTTCCGAGGTAG+AGG + chr2_2:64680552-64680571 Msa0264740:intron 40.0%
! CCAAATTTTCACCTCTACCT+CGG - chr2_2:64680566-64680585 None:intergenic 40.0%
CGAGGTAGAGGTGAAAATTT+GGG + chr2_2:64680564-64680583 Msa0264740:intron 40.0%
CATGTTAGTCAACTGGTAGA+TGG + chr2_2:64680795-64680814 Msa0264740:intron 40.0%
AGACGACGATAACAAGTATG+AGG + chr2_2:64680984-64681003 Msa0264740:intron 40.0%
CAGTTGTTGATGCACAACAT+AGG - chr2_2:64681665-64681684 None:intergenic 40.0%
ATGTTGTGCATCAACAACTG+TGG + chr2_2:64681665-64681684 Msa0264740:three_prime_UTR 40.0%
GTTTCAGCATCATGTCTTTG+TGG - chr2_2:64681733-64681752 None:intergenic 40.0%
CAAAGACATGATGCTGAAAC+AGG + chr2_2:64681733-64681752 Msa0264740:three_prime_UTR 40.0%
TCCATTGGGAACATCATGAA+TGG + chr2_2:64681779-64681798 Msa0264740:three_prime_UTR 40.0%
CCATTGGGAACATCATGAAT+GGG + chr2_2:64681780-64681799 Msa0264740:three_prime_UTR 40.0%
AAAAGCAAACCCTGTTGTGA+AGG + chr2_2:64682093-64682112 Msa0264740:three_prime_UTR 40.0%
! TTTATATGTGATGGGGGACT+TGG + chr2_2:64682242-64682261 Msa0264740:three_prime_UTR 40.0%
! TTATATGTGATGGGGGACTT+GGG + chr2_2:64682243-64682262 Msa0264740:three_prime_UTR 40.0%
! TCTGCTAGTTTAGTGGAAGT+TGG + chr2_2:64682349-64682368 Msa0264740:three_prime_UTR 40.0%
!! TTGGTGGTGTCTGCATTATA+AGG + chr2_2:64682368-64682387 Msa0264740:three_prime_UTR 40.0%
AAGAATGCGTTGAATGTGCT+TGG + chr2_2:64682541-64682560 Msa0264740:three_prime_UTR 40.0%
AGAATGCGTTGAATGTGCTT+GGG + chr2_2:64682542-64682561 Msa0264740:three_prime_UTR 40.0%
! ACGAACGCTCTTCACTGATT+GGG - chr2_2:64680100-64680119 None:intergenic 45.0%
AACGAACGCTCTTCACTGAT+TGG - chr2_2:64680101-64680120 None:intergenic 45.0%
!! AATCAGTGAAGAGCGTTCGT+TGG + chr2_2:64680100-64680119 Msa0264740:CDS 45.0%
CCGAGGTAGAGGTGAAAATT+TGG + chr2_2:64680563-64680582 Msa0264740:intron 45.0%
AAGATGGACCATGATCAGAC+TGG + chr2_2:64681617-64681636 Msa0264740:three_prime_UTR 45.0%
CTTCACATCCAGTCTGATCA+TGG - chr2_2:64681628-64681647 None:intergenic 45.0%
GCACTTGGAACACATGTTCA+TGG - chr2_2:64681711-64681730 None:intergenic 45.0%
CATCATGTCTTTGTGGCACT+TGG - chr2_2:64681726-64681745 None:intergenic 45.0%
AATGGAAGATGCTGCAAGTG+TGG - chr2_2:64681765-64681784 None:intergenic 45.0%
ACACTTGCAGCATCTTCCAT+TGG + chr2_2:64681764-64681783 Msa0264740:three_prime_UTR 45.0%
CACTTGCAGCATCTTCCATT+GGG + chr2_2:64681765-64681784 Msa0264740:three_prime_UTR 45.0%
CCCATTCATGATGTTCCCAA+TGG - chr2_2:64681783-64681802 None:intergenic 45.0%
AGGTTCAATTACACTGCTGC+TGG - chr2_2:64681810-64681829 None:intergenic 45.0%
! GGTTCTGCAGAGATGGTTTT+GGG - chr2_2:64681875-64681894 None:intergenic 45.0%
AACTAACGGTTCTGCAGAGA+TGG - chr2_2:64681882-64681901 None:intergenic 45.0%
!! AATAAGCGGGTTGGTTTGAC+AGG + chr2_2:64681968-64681987 Msa0264740:three_prime_UTR 45.0%
AGCGATGTACAGCACAGTAA+AGG - chr2_2:64682015-64682034 None:intergenic 45.0%
CAGCAGTGCGGTAATCAAAT+GGG - chr2_2:64682057-64682076 None:intergenic 45.0%
! GCTTTTCAGCCTTCACAACA+GGG - chr2_2:64682105-64682124 None:intergenic 45.0%
! AGCTTTTCAGCCTTCACAAC+AGG - chr2_2:64682106-64682125 None:intergenic 45.0%
! GCTAGTTTAGTGGAAGTTGG+TGG + chr2_2:64682352-64682371 Msa0264740:three_prime_UTR 45.0%
GGGAATGGTTATTGGGGTTG+AGG - chr2_2:64680067-64680086 None:intergenic 50.0%
GACGTGGGGAATGGTTATTG+GGG - chr2_2:64680073-64680092 None:intergenic 50.0%
CGACGTGGGGAATGGTTATT+GGG - chr2_2:64680074-64680093 None:intergenic 50.0%
! CGAACGCTCTTCACTGATTG+GGG - chr2_2:64680099-64680118 None:intergenic 50.0%
GGTTGGATATATTCGCGTCG+TGG + chr2_2:64680463-64680482 Msa0264740:intron 50.0%
TGCACAACATAGGACCCTCT+GGG - chr2_2:64681655-64681674 None:intergenic 50.0%
ATGCACAACATAGGACCCTC+TGG - chr2_2:64681656-64681675 None:intergenic 50.0%
CATGATGCTGAAACAGGAGC+AGG + chr2_2:64681739-64681758 Msa0264740:three_prime_UTR 50.0%
GGAGATCTCAGTCGATCAAG+TGG + chr2_2:64681847-64681866 Msa0264740:three_prime_UTR 50.0%
! CGGTTCTGCAGAGATGGTTT+TGG - chr2_2:64681876-64681895 None:intergenic 50.0%
GCAGAACCGTTAGTTGCTTC+CGG + chr2_2:64681887-64681906 Msa0264740:three_prime_UTR 50.0%
TCTGAGCCGGAAGCAACTAA+CGG - chr2_2:64681896-64681915 None:intergenic 50.0%
GAGTTTGGAGAAGAAGCCGA+AGG + chr2_2:64681919-64681938 Msa0264740:three_prime_UTR 50.0%
TTGGAGAAGAAGCCGAAGGA+TGG + chr2_2:64681923-64681942 Msa0264740:three_prime_UTR 50.0%
GGTGCAGCAACTGCAATAAG+CGG + chr2_2:64681954-64681973 Msa0264740:three_prime_UTR 50.0%
GTGCAGCAACTGCAATAAGC+GGG + chr2_2:64681955-64681974 Msa0264740:three_prime_UTR 50.0%
AGCAACTGCAATAAGCGGGT+TGG + chr2_2:64681959-64681978 Msa0264740:three_prime_UTR 50.0%
CCAGCAGTGCGGTAATCAAA+TGG - chr2_2:64682058-64682077 None:intergenic 50.0%
CCATTTGATTACCGCACTGC+TGG + chr2_2:64682055-64682074 Msa0264740:three_prime_UTR 50.0%
GCGACGTGGGGAATGGTTAT+TGG - chr2_2:64680075-64680094 None:intergenic 55.0%
GGGATCGAAGAGGGAGAAAG+AGG - chr2_2:64680226-64680245 None:intergenic 55.0%
GCACAACATAGGACCCTCTG+GGG - chr2_2:64681654-64681673 None:intergenic 55.0%
ATCGACTGAGATCTCCACGC+TGG - chr2_2:64681843-64681862 None:intergenic 55.0%
GTTAGTTGCTTCCGGCTCAG+AGG + chr2_2:64681895-64681914 Msa0264740:three_prime_UTR 55.0%
TTCCGGCTCAGAGGAGAGTT+TGG + chr2_2:64681904-64681923 Msa0264740:three_prime_UTR 55.0%
CTCCAAACTCTCCTCTGAGC+CGG - chr2_2:64681909-64681928 None:intergenic 55.0%
AGCCGAAGGATGGTCCTAAG+CGG + chr2_2:64681933-64681952 Msa0264740:three_prime_UTR 55.0%
CACCGCTTAGGACCATCCTT+CGG - chr2_2:64681938-64681957 None:intergenic 55.0%
TTGCAGTTGCTGCACCGCTT+AGG - chr2_2:64681950-64681969 None:intergenic 55.0%
TTGCATCACGTCCAGCAGTG+CGG - chr2_2:64682069-64682088 None:intergenic 55.0%
ACTGATTGGGGTGCGACGTG+GGG - chr2_2:64680087-64680106 None:intergenic 60.0%
! CACTGATTGGGGTGCGACGT+GGG - chr2_2:64680088-64680107 None:intergenic 60.0%
! TCACTGATTGGGGTGCGACG+TGG - chr2_2:64680089-64680108 None:intergenic 60.0%
TGGATGTGAAGCTGCCCCAG+AGG + chr2_2:64681637-64681656 Msa0264740:three_prime_UTR 60.0%
GGATGTGAAGCTGCCCCAGA+GGG + chr2_2:64681638-64681657 Msa0264740:three_prime_UTR 60.0%
ACCTGCTATCACTGCCAGCG+TGG + chr2_2:64681826-64681845 Msa0264740:three_prime_UTR 60.0%
TCCACGCTGGCAGTGATAGC+AGG - chr2_2:64681830-64681849 None:intergenic 60.0%
TTGGGGTGCGACGTGGGGAA+TGG - chr2_2:64680082-64680101 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 64680051 64682603 64680051 ID=Msa0264740;Name=Msa0264740
chr2_2 mRNA 64680051 64682603 64680051 ID=Msa0264740-mRNA-1;Parent=Msa0264740;Name=Msa0264740-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0.5|1|0|0|2|990|71
chr2_2 exon 64680051 64680257 64680051 ID=Msa0264740-mRNA-1:exon:20209;Parent=Msa0264740-mRNA-1
chr2_2 exon 64681605 64682603 64681605 ID=Msa0264740-mRNA-1:exon:20210;Parent=Msa0264740-mRNA-1
chr2_2 CDS 64680051 64680257 64680051 ID=Msa0264740-mRNA-1:cds;Parent=Msa0264740-mRNA-1
chr2_2 CDS 64681605 64681613 64681605 ID=Msa0264740-mRNA-1:cds;Parent=Msa0264740-mRNA-1
chr2_2 three_prime_UTR 64681614 64682603 64681614 ID=Msa0264740-mRNA-1:three_prime_utr;Parent=Msa0264740-mRNA-1
Gene Sequence

>Msa0264740

ATGAAACATGAAACCTCAACCCCAATAACCATTCCCCACGTCGCACCCCAATCAGTGAAGAGCGTTCGTTGGCAAAACAAAGAAAGAGAAACAAAGCTTTTCAAACACACAAAAAAAAAAAAAGGAGAGAGAAACAAACACTCACAGAACTCATTTTCATTTCTTTCTCTCTCCTCTTTCTCCCTCTTCGATCCCAAATCTTCAAAGGAAAGTTGA
Protein sequence

>Msa0264740

MKHETSTPITIPHVAPQSVKSVRWQNKERETKLFKHTKKKKGERNKHSQNSFSFLSLSSFSLFDPKSSKES*