Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0271480 | AES63355.1 | 93.043 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 1.76e-71 | 219 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0271480 | G7IL67 | 93.043 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 8.39e-72 | 219 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0189460 | Msa0271480 | 0.829955 | 3.852287e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0271480 | MtrunA17_Chr2g0278461 | 93.043 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 1.62e-75 | 219 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGACCTAATTCTCATTAA+AGG | 0.208207 | 2_3:-2860798 | None:intergenic |
| ACCTCCCTTTAATGAGAATT+AGG | 0.311240 | 2_3:+2860793 | Msa0271480:CDS |
| TGCTATTACTAAGTTCTATT+AGG | 0.322038 | 2_3:-2860544 | None:intergenic |
| ATCGAAGACGCAACAGAGTA+AGG | 0.399303 | 2_3:+2860622 | Msa0271480:CDS |
| GTGACCTAATTCTCATTAAA+GGG | 0.412376 | 2_3:-2860797 | None:intergenic |
| CGCTGCATCAATTTCCGTTA+GGG | 0.412692 | 2_3:-2860516 | None:intergenic |
| AAGGGAGGTAAAATAACTAT+AGG | 0.423320 | 2_3:-2860779 | None:intergenic |
| TTGAAGAAGATGAACGGTTA+AGG | 0.434763 | 2_3:+2860705 | Msa0271480:CDS |
| TCGCTGCATCAATTTCCGTT+AGG | 0.435545 | 2_3:-2860517 | None:intergenic |
| GGTTAAGGAGGAAGAATAAG+AGG | 0.436235 | 2_3:+2860720 | Msa0271480:CDS |
| TAGTAATAGCACAGCCTACT+TGG | 0.455809 | 2_3:+2860556 | Msa0271480:CDS |
| CGCTGTTGCTGCTATTGCTA+TGG | 0.460075 | 2_3:-2860589 | None:intergenic |
| CACATCCTCAACTGAATCTG+TGG | 0.495772 | 2_3:-2860672 | None:intergenic |
| ACTGAATCTGTGGGTGATGA+TGG | 0.517018 | 2_3:-2860662 | None:intergenic |
| AGATTCAGTTGAGGATGTGT+TGG | 0.537928 | 2_3:+2860676 | Msa0271480:CDS |
| AAGAAGATGAACGGTTAAGG+AGG | 0.558290 | 2_3:+2860708 | Msa0271480:CDS |
| CAGAAATTGAAGAAGATGAA+CGG | 0.588402 | 2_3:+2860699 | Msa0271480:CDS |
| TCGAAGACGCAACAGAGTAA+GGG | 0.588668 | 2_3:+2860623 | Msa0271480:CDS |
| CAATAGCAGCAACAGCGTCA+CGG | 0.606320 | 2_3:+2860595 | Msa0271480:CDS |
| TATGTTGAAGAAGTGTATAG+AGG | 0.618180 | 2_3:+2860749 | Msa0271480:CDS |
| ACATCCTCAACTGAATCTGT+GGG | 0.636514 | 2_3:-2860671 | None:intergenic |
| ACCTAATTCTCATTAAAGGG+AGG | 0.645761 | 2_3:-2860794 | None:intergenic |
| ATCACCCACAGATTCAGTTG+AGG | 0.662122 | 2_3:+2860667 | Msa0271480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAACCATACTTTTTCTTCT+GGG | - | chr2_3:2860492-2860511 | None:intergenic | 25.0% |
| !!! | AAAAACCATACTTTTTCTTC+TGG | - | chr2_3:2860493-2860512 | None:intergenic | 25.0% |
| ! | TGCTATTACTAAGTTCTATT+AGG | - | chr2_3:2860547-2860566 | None:intergenic | 25.0% |
| !!! | AAACCATACTTTTTCTTCTG+GGG | - | chr2_3:2860491-2860510 | None:intergenic | 30.0% |
| CAGAAATTGAAGAAGATGAA+CGG | + | chr2_3:2860699-2860718 | Msa0271480:CDS | 30.0% | |
| ! | TATGTTGAAGAAGTGTATAG+AGG | + | chr2_3:2860749-2860768 | Msa0271480:CDS | 30.0% |
| AAGGGAGGTAAAATAACTAT+AGG | - | chr2_3:2860782-2860801 | None:intergenic | 30.0% | |
| !!! | AACCATACTTTTTCTTCTGG+GGG | - | chr2_3:2860490-2860509 | None:intergenic | 35.0% |
| !!! | GTATGGTTTTTTCTCCCTAA+CGG | + | chr2_3:2860502-2860521 | Msa0271480:CDS | 35.0% |
| ! | ATGGCAATTTTCATCCAAGT+AGG | - | chr2_3:2860573-2860592 | None:intergenic | 35.0% |
| TTGAAGAAGATGAACGGTTA+AGG | + | chr2_3:2860705-2860724 | Msa0271480:CDS | 35.0% | |
| AACCCCCAGAAGAAAAAGTA+TGG | + | chr2_3:2860485-2860504 | Msa0271480:CDS | 40.0% | |
| TAGTAATAGCACAGCCTACT+TGG | + | chr2_3:2860556-2860575 | Msa0271480:CDS | 40.0% | |
| ACATCCTCAACTGAATCTGT+GGG | - | chr2_3:2860674-2860693 | None:intergenic | 40.0% | |
| AGATTCAGTTGAGGATGTGT+TGG | + | chr2_3:2860676-2860695 | Msa0271480:CDS | 40.0% | |
| AAGAAGATGAACGGTTAAGG+AGG | + | chr2_3:2860708-2860727 | Msa0271480:CDS | 40.0% | |
| GGTTAAGGAGGAAGAATAAG+AGG | + | chr2_3:2860720-2860739 | Msa0271480:CDS | 40.0% | |
| CGCTGCATCAATTTCCGTTA+GGG | - | chr2_3:2860519-2860538 | None:intergenic | 45.0% | |
| TCGCTGCATCAATTTCCGTT+AGG | - | chr2_3:2860520-2860539 | None:intergenic | 45.0% | |
| ATCGAAGACGCAACAGAGTA+AGG | + | chr2_3:2860622-2860641 | Msa0271480:CDS | 45.0% | |
| TCGAAGACGCAACAGAGTAA+GGG | + | chr2_3:2860623-2860642 | Msa0271480:CDS | 45.0% | |
| ACTGAATCTGTGGGTGATGA+TGG | - | chr2_3:2860665-2860684 | None:intergenic | 45.0% | |
| ATCACCCACAGATTCAGTTG+AGG | + | chr2_3:2860667-2860686 | Msa0271480:CDS | 45.0% | |
| CACATCCTCAACTGAATCTG+TGG | - | chr2_3:2860675-2860694 | None:intergenic | 45.0% | |
| CGCTGTTGCTGCTATTGCTA+TGG | - | chr2_3:2860592-2860611 | None:intergenic | 50.0% | |
| CAATAGCAGCAACAGCGTCA+CGG | + | chr2_3:2860595-2860614 | Msa0271480:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 2860467 | 2860814 | 2860467 | ID=Msa0271480;Name=Msa0271480 |
| chr2_3 | mRNA | 2860467 | 2860814 | 2860467 | ID=Msa0271480-mRNA-1;Parent=Msa0271480;Name=Msa0271480-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|115 |
| chr2_3 | exon | 2860467 | 2860814 | 2860467 | ID=Msa0271480-mRNA-1:exon:1367;Parent=Msa0271480-mRNA-1 |
| chr2_3 | CDS | 2860467 | 2860814 | 2860467 | ID=Msa0271480-mRNA-1:cds;Parent=Msa0271480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
