Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189460 | AES63355.1 | 91.304 | 115 | 10 | 0 | 1 | 115 | 1 | 115 | 9.24e-70 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189460 | G7IL67 | 91.304 | 115 | 10 | 0 | 1 | 115 | 1 | 115 | 4.41e-70 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0138020 | Msa0189460 | 0.808423 | 3.119580e-50 | -8.615850e-47 |
| Msa0189460 | Msa0271480 | 0.829955 | 3.852287e-55 | -8.615850e-47 |
| Msa0189460 | Msa0689780 | 0.814780 | 1.294296e-51 | -8.615850e-47 |
| Msa0189460 | Msa0844110 | 0.812556 | 3.995782e-51 | -8.615850e-47 |
| Msa0189460 | Msa1063420 | 0.805551 | 1.263900e-49 | -8.615850e-47 |
| Msa0189460 | Msa1207310 | 0.805738 | 1.154835e-49 | -8.615850e-47 |
| Msa0189460 | Msa1270660 | 0.819612 | 1.059875e-52 | -8.615850e-47 |
| Msa0189460 | Msa1270730 | 0.815040 | 1.133469e-51 | -8.615850e-47 |
| Msa0189460 | Msa1332050 | 0.801909 | 7.206536e-49 | -8.615850e-47 |
| Msa0189460 | Msa1335230 | 0.803887 | 2.812400e-49 | -8.615850e-47 |
| Msa0189460 | Msa1420270 | 0.809753 | 1.619215e-50 | -8.615850e-47 |
| Msa0189460 | Msa1423820 | 0.803261 | 3.793759e-49 | -8.615850e-47 |
| Msa0189460 | Msa1444260 | 0.815871 | 7.404029e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0189460 | MtrunA17_Chr2g0278461 | 91.304 | 115 | 10 | 0 | 1 | 115 | 1 | 115 | 8.50e-74 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGACCTAATTCTCATTAA+AGG | 0.176232 | 2_1:+4848487 | None:intergenic |
| ACCTCCCTTTAATGAGAATT+AGG | 0.311240 | 2_1:-4848492 | None:intergenic |
| TGCTATTACTAAGTTCTATT+AGG | 0.322038 | 2_1:+4848741 | None:intergenic |
| GTGACCTAATTCTCATTAAA+GGG | 0.385524 | 2_1:+4848488 | None:intergenic |
| ATCGAAGACGCAACAGAGTA+AGG | 0.399303 | 2_1:-4848663 | Msa0189460:CDS |
| CGCTGCATCAATTTCCGTTA+GGG | 0.412692 | 2_1:+4848769 | None:intergenic |
| AAGGGAGGTAAAATAACTAT+AGG | 0.423320 | 2_1:+4848506 | None:intergenic |
| TTGAAGAAGATGAACGGTTA+AGG | 0.434763 | 2_1:-4848580 | Msa0189460:CDS |
| TCGCTGCATCAATTTCCGTT+AGG | 0.435545 | 2_1:+4848768 | None:intergenic |
| GGTTAAGGAGGAAGAATAAG+AGG | 0.436235 | 2_1:-4848565 | Msa0189460:CDS |
| TAGTAATAGCACAGCCTACT+TGG | 0.455809 | 2_1:-4848729 | Msa0189460:CDS |
| CGCTGTTGCTGCTATTGCTA+TGG | 0.460075 | 2_1:+4848696 | None:intergenic |
| CACATCCTCAACTGAATCTG+TGG | 0.495772 | 2_1:+4848613 | None:intergenic |
| ACTGAATCTGTGGGTGATGA+TGG | 0.517018 | 2_1:+4848623 | None:intergenic |
| AGATTCAGTTGAGGATGTGT+TGG | 0.537928 | 2_1:-4848609 | Msa0189460:CDS |
| AAGAAGATGAACGGTTAAGG+AGG | 0.558290 | 2_1:-4848577 | Msa0189460:CDS |
| CAGAAATTGAAGAAGATGAA+CGG | 0.588402 | 2_1:-4848586 | Msa0189460:CDS |
| TCGAAGACGCAACAGAGTAA+GGG | 0.588668 | 2_1:-4848662 | Msa0189460:CDS |
| CAATAGCAGCAACAGCGTCA+CGG | 0.606320 | 2_1:-4848690 | Msa0189460:CDS |
| TATGTTGAAGAAGTGTATAG+AGG | 0.618180 | 2_1:-4848536 | Msa0189460:CDS |
| ACATCCTCAACTGAATCTGT+GGG | 0.636514 | 2_1:+4848614 | None:intergenic |
| ACCTAATTCTCATTAAAGGG+AGG | 0.645761 | 2_1:+4848491 | None:intergenic |
| ATCACCCACAGATTCAGTTG+AGG | 0.662122 | 2_1:-4848618 | Msa0189460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACCATACTTTTTCTTCTT+GGG | + | chr2_1:4848517-4848536 | None:intergenic | 25.0% |
| !!! | AAAACCATACTTTTTCTTCT+TGG | + | chr2_1:4848518-4848537 | None:intergenic | 25.0% |
| ! | TGCTATTACTAAGTTCTATT+AGG | + | chr2_1:4848573-4848592 | None:intergenic | 25.0% |
| !!! | AACCATACTTTTTCTTCTTG+GGG | + | chr2_1:4848516-4848535 | None:intergenic | 30.0% |
| CAGAAATTGAAGAAGATGAA+CGG | - | chr2_1:4848725-4848744 | Msa0189460:CDS | 30.0% | |
| ! | TATGTTGAAGAAGTGTATAG+AGG | - | chr2_1:4848775-4848794 | Msa0189460:CDS | 30.0% |
| AAGGGAGGTAAAATAACTAT+AGG | + | chr2_1:4848808-4848827 | None:intergenic | 30.0% | |
| AACCCCAAGAAGAAAAAGTA+TGG | - | chr2_1:4848511-4848530 | Msa0189460:CDS | 35.0% | |
| !!! | GTATGGTTTTTTCTCCCTAA+CGG | - | chr2_1:4848528-4848547 | Msa0189460:CDS | 35.0% |
| ! | ATGGCAATTTTCATCCAAGT+AGG | + | chr2_1:4848599-4848618 | None:intergenic | 35.0% |
| TTGAAGAAGATGAACGGTTA+AGG | - | chr2_1:4848731-4848750 | Msa0189460:CDS | 35.0% | |
| TAGTAATAGCACAGCCTACT+TGG | - | chr2_1:4848582-4848601 | Msa0189460:CDS | 40.0% | |
| ACATCCTCAACTGAATCTGT+GGG | + | chr2_1:4848700-4848719 | None:intergenic | 40.0% | |
| AGATTCAGTTGAGGATGTGT+TGG | - | chr2_1:4848702-4848721 | Msa0189460:CDS | 40.0% | |
| AAGAAGATGAACGGTTAAGG+AGG | - | chr2_1:4848734-4848753 | Msa0189460:CDS | 40.0% | |
| GGTTAAGGAGGAAGAATAAG+AGG | - | chr2_1:4848746-4848765 | Msa0189460:CDS | 40.0% | |
| CGCTGCATCAATTTCCGTTA+GGG | + | chr2_1:4848545-4848564 | None:intergenic | 45.0% | |
| TCGCTGCATCAATTTCCGTT+AGG | + | chr2_1:4848546-4848565 | None:intergenic | 45.0% | |
| ATCGAAGACGCAACAGAGTA+AGG | - | chr2_1:4848648-4848667 | Msa0189460:CDS | 45.0% | |
| TCGAAGACGCAACAGAGTAA+GGG | - | chr2_1:4848649-4848668 | Msa0189460:CDS | 45.0% | |
| ACTGAATCTGTGGGTGATGA+TGG | + | chr2_1:4848691-4848710 | None:intergenic | 45.0% | |
| ATCACCCACAGATTCAGTTG+AGG | - | chr2_1:4848693-4848712 | Msa0189460:CDS | 45.0% | |
| CACATCCTCAACTGAATCTG+TGG | + | chr2_1:4848701-4848720 | None:intergenic | 45.0% | |
| CGCTGTTGCTGCTATTGCTA+TGG | + | chr2_1:4848618-4848637 | None:intergenic | 50.0% | |
| CAATAGCAGCAACAGCGTCA+CGG | - | chr2_1:4848621-4848640 | Msa0189460:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 4848493 | 4848840 | 4848493 | ID=Msa0189460;Name=Msa0189460 |
| chr2_1 | mRNA | 4848493 | 4848840 | 4848493 | ID=Msa0189460-mRNA-1;Parent=Msa0189460;Name=Msa0189460-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|115 |
| chr2_1 | exon | 4848493 | 4848840 | 4848493 | ID=Msa0189460-mRNA-1:exon:2170;Parent=Msa0189460-mRNA-1 |
| chr2_1 | CDS | 4848493 | 4848840 | 4848493 | ID=Msa0189460-mRNA-1:cds;Parent=Msa0189460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
