AlfalfaGEDB | Msa0272460

M. sativa cultivar zhongmu-4 / Msa0272460

KEGG: -
GO: -
PFAM: PF02701.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0272460	XP_003593557.1	92.058	277	18	2	1	276	1	274	1.65e-170	482

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0272460	sp\|Q9FZA4\|DOF14_ARATH	65.060	83	28	1	20	101	1	83	3.58e-28	112

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0272460	G7IPR3	92.058	277	18	2	1	276	1	274	7.90e-171	482

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TFs/TRs:

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Gene ID	Type	Classification
Msa0272460	TF	C2C2-Dof

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0272460	Msa0312660	0.927125	1.816932e-91	-8.615850e-47
Msa0272460	Msa1386060	0.991907	3.321674e-190	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0272460	MtrunA17_Chr2g0282051	92.058	277	18	2	1	276	1	274	1.52e-174	482
Msa0272460	MtrunA17_Chr4g0047761	55.290	293	96	10	12	276	35	320	5.87e-81	246
Msa0272460	MtrunA17_Chr3g0127201	66.197	71	23	1	30	99	11	81	6.96e-29	111
Msa0272460	MtrunA17_Chr4g0047001	63.415	82	29	1	48	128	57	138	1.00e-28	112
Msa0272460	MtrunA17_Chr2g0328021	46.212	132	58	4	46	176	62	181	1.39e-28	110
Msa0272460	MtrunA17_Chr8g0374251	59.036	83	31	2	39	120	63	143	1.59e-28	111
Msa0272460	MtrunA17_Chr8g0374111	58.750	80	27	3	44	121	27	102	4.98e-27	105
Msa0272460	MtrunA17_Chr7g0223311	67.568	74	19	2	38	106	69	142	6.43e-27	107
Msa0272460	MtrunA17_Chr8g0346551	58.108	74	30	1	28	100	6	79	9.32e-27	105
Msa0272460	MtrunA17_Chr2g0326001	77.193	57	12	1	44	99	38	94	1.42e-26	105

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0272460	AT1G28310.1	65.060	83	28	1	20	101	1	83	3.64e-29	112
Msa0272460	AT1G28310.2	65.060	83	28	1	20	101	25	107	7.08e-29	112
Msa0272460	AT5G66940.1	60.274	73	27	1	42	112	28	100	9.50e-28	107
Msa0272460	AT5G60850.1	59.770	87	31	2	34	116	36	122	1.86e-27	108
Msa0272460	AT1G51700.1	38.750	160	85	4	33	179	16	175	1.98e-27	105
Msa0272460	AT5G60200.1	57.447	94	32	3	19	111	32	118	2.94e-27	106
Msa0272460	AT3G55370.2	66.667	66	21	1	45	109	75	140	2.95e-27	108
Msa0272460	AT3G55370.1	66.667	66	21	1	45	109	75	140	2.96e-27	108
Msa0272460	AT3G55370.3	66.667	66	21	1	45	109	120	185	3.32e-27	108
Msa0272460	AT3G61850.2	75.439	57	13	1	44	99	60	116	1.97e-26	105

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Find 62 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGCTTGAGTTTGAGAA+TGG	0.415643	2_3:-3994936	None:intergenic
AACAATGAAGAATTTGGTAT+TGG	0.395188	2_3:+3994855	Msa0272460:CDS
AACTGATCTAAATGTTCCTT+GGG	0.432000	2_3:+3995148	Msa0272460:CDS
AAGAGACATTGGACTAAAGG+TGG	0.661565	2_3:+3994591	Msa0272460:CDS
AAGAGCTTGAGTTTGAGAAT+GGG	0.526214	2_3:-3994935	None:intergenic
ACCTGCAACAAGTAGTGGCA+TGG	0.573077	2_3:+3995079	Msa0272460:CDS
ACTCTCAGGAATGTTCCTGT+TGG	0.606251	2_3:+3994615	Msa0272460:CDS
AGAATGGGAATATAAAGCTT+TGG	0.413450	2_3:-3994920	None:intergenic
AGAGATTCATGCATAGAAGA+AGG	0.563751	2_3:-3994768	None:intergenic
AGCTCTTTAAGATCATCCAA+TGG	0.640870	2_3:+3994951	Msa0272460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATGGGAATATAAAGCTT+TGG	-	chr2_3:3994923-3994942	None:intergenic	30.0%
TCATCCAATGGATATAACTA+TGG	+	chr2_3:3994963-3994982	Msa0272460:CDS	30.0%
TTCACCATAGTTATATCCAT+TGG	-	chr2_3:3994970-3994989	None:intergenic	30.0%
ATATGTCAAACTATTGGAGT+TGG	+	chr2_3:3995102-3995121	Msa0272460:CDS	30.0%
TATGTCAAACTATTGGAGTT+GGG	+	chr2_3:3995103-3995122	Msa0272460:CDS	30.0%
AACTGATCTAAATGTTCCTT+GGG	+	chr2_3:3995148-3995167	Msa0272460:CDS	30.0%
AGAATGGGAATATAAAGCTT+TGG	-	chr2_3:3994923-3994942	None:intergenic	30.0%
TCATCCAATGGATATAACTA+TGG	+	chr2_3:3994963-3994982	Msa0272460:CDS	30.0%
TTCACCATAGTTATATCCAT+TGG	-	chr2_3:3994970-3994989	None:intergenic	30.0%
ATATGTCAAACTATTGGAGT+TGG	+	chr2_3:3995102-3995121	Msa0272460:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	3994363	3995193	3994363	ID=Msa0272460;Name=Msa0272460
chr2_3	mRNA	3994363	3995193	3994363	ID=Msa0272460-mRNA-1;Parent=Msa0272460;Name=Msa0272460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|276
chr2_3	exon	3994363	3995193	3994363	ID=Msa0272460-mRNA-1:exon:2018;Parent=Msa0272460-mRNA-1
chr2_3	CDS	3994363	3995193	3994363	ID=Msa0272460-mRNA-1:cds;Parent=Msa0272460-mRNA-1

Gene Sequence

>Msa0272460

ATGGGCTTGAGTTCTAAGCAAGTTTCTAGTAGAGGTGTTGATTGGAACCAAACCTTGTTGCAAGATCAAAACATGGAACTTCCAAAGACTCCTCCCATGAGAAAACAACAGCAACAAAATCAGCAACTAGAGGCAGTGAATTGTCCAAGATGTGACTCTACAAATACCAAATTTTGTTACTACAACAACTATAACAAGTCTCAACCTCGCCATTTCTGCAGAGCTTGCAAGAGACATTGGACTAAAGGTGGTACTCTCAGGAATGTTCCTGTTGGTGGTGTTAGGAAGAATAAGAGAGTCAAGATATCAACTTCTCCAACTTCTATTAGCATCACCTCAAAAACTACTATTAGCACCTCCTCCACCACCACCTCAAATGACAACAGGCAGAATAAAAAAACCACACCTTCTTCTATGCATGAATCTCTTATTCATCCACCATCTTTGTTGCCACCACAGAATCTAATGAGTACCAAAGATCAATCAGGTGGTAACAATGAAGAATTTGGTATTGGTAATGACATGTTTCTGAGTTCATCTATGCCTTTTCCTCAAAACCAAAGCTTTATATTCCCATTCTCAAACTCAAGCTCTTTAAGATCATCCAATGGATATAACTATGGTGAAGAGTTTAAAATAATGGAGGAGTCAACTATTAACAACAGTGCAATGCCAAGCACTAGTGGTAGTGGCACTATTACACATCCATGGGAGATACCTGCAACAAGTAGTGGCATGGATATGTCAAACTATTGGAGTTGGGAGGATATTGATTCTTTGGTCTCAACTGATCTAAATGTTCCTTGGGATGATTCTGATATCAAACCTTGA

Protein sequence

>Msa0272460

MGLSSKQVSSRGVDWNQTLLQDQNMELPKTPPMRKQQQQNQQLEAVNCPRCDSTNTKFCYYNNYNKSQPRHFCRACKRHWTKGGTLRNVPVGGVRKNKRVKISTSPTSISITSKTTISTSSTTTSNDNRQNKKTTPSSMHESLIHPPSLLPPQNLMSTKDQSGGNNEEFGIGNDMFLSSSMPFPQNQSFIFPFSNSSSLRSSNGYNYGEEFKIMEESTINNSAMPSTSGSGTITHPWEIPATSSGMDMSNYWSWEDIDSLVSTDLNVPWDDSDIKP*