Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0272460 | XP_003593557.1 | 92.058 | 277 | 18 | 2 | 1 | 276 | 1 | 274 | 1.65e-170 | 482 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0272460 | sp|Q9FZA4|DOF14_ARATH | 65.060 | 83 | 28 | 1 | 20 | 101 | 1 | 83 | 3.58e-28 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0272460 | G7IPR3 | 92.058 | 277 | 18 | 2 | 1 | 276 | 1 | 274 | 7.90e-171 | 482 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0272460 | TF | C2C2-Dof |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0272460 | Msa0312660 | 0.927125 | 1.816932e-91 | -8.615850e-47 |
| Msa0272460 | Msa1386060 | 0.991907 | 3.321674e-190 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0272460 | MtrunA17_Chr2g0282051 | 92.058 | 277 | 18 | 2 | 1 | 276 | 1 | 274 | 1.52e-174 | 482 |
| Msa0272460 | MtrunA17_Chr4g0047761 | 55.290 | 293 | 96 | 10 | 12 | 276 | 35 | 320 | 5.87e-81 | 246 |
| Msa0272460 | MtrunA17_Chr3g0127201 | 66.197 | 71 | 23 | 1 | 30 | 99 | 11 | 81 | 6.96e-29 | 111 |
| Msa0272460 | MtrunA17_Chr4g0047001 | 63.415 | 82 | 29 | 1 | 48 | 128 | 57 | 138 | 1.00e-28 | 112 |
| Msa0272460 | MtrunA17_Chr2g0328021 | 46.212 | 132 | 58 | 4 | 46 | 176 | 62 | 181 | 1.39e-28 | 110 |
| Msa0272460 | MtrunA17_Chr8g0374251 | 59.036 | 83 | 31 | 2 | 39 | 120 | 63 | 143 | 1.59e-28 | 111 |
| Msa0272460 | MtrunA17_Chr8g0374111 | 58.750 | 80 | 27 | 3 | 44 | 121 | 27 | 102 | 4.98e-27 | 105 |
| Msa0272460 | MtrunA17_Chr7g0223311 | 67.568 | 74 | 19 | 2 | 38 | 106 | 69 | 142 | 6.43e-27 | 107 |
| Msa0272460 | MtrunA17_Chr8g0346551 | 58.108 | 74 | 30 | 1 | 28 | 100 | 6 | 79 | 9.32e-27 | 105 |
| Msa0272460 | MtrunA17_Chr2g0326001 | 77.193 | 57 | 12 | 1 | 44 | 99 | 38 | 94 | 1.42e-26 | 105 |
| Msa0272460 | MtrunA17_Chr2g0282511 | 83.673 | 49 | 8 | 0 | 45 | 93 | 44 | 92 | 1.80e-26 | 105 |
| Msa0272460 | MtrunA17_Chr4g0031411 | 55.682 | 88 | 33 | 2 | 45 | 127 | 74 | 160 | 1.95e-26 | 105 |
| Msa0272460 | MtrunA17_Chr8g0367161 | 60.870 | 69 | 26 | 1 | 40 | 107 | 26 | 94 | 2.04e-26 | 105 |
| Msa0272460 | MtrunA17_Chr8g0341671 | 60.811 | 74 | 22 | 1 | 20 | 93 | 1 | 67 | 3.35e-26 | 102 |
| Msa0272460 | MtrunA17_Chr4g0010151 | 53.846 | 91 | 34 | 2 | 45 | 127 | 81 | 171 | 3.51e-26 | 105 |
| Msa0272460 | MtrunA17_Chr1g0188851 | 59.420 | 69 | 27 | 1 | 32 | 99 | 29 | 97 | 4.47e-26 | 103 |
| Msa0272460 | MtrunA17_Chr3g0126201 | 74.138 | 58 | 14 | 1 | 45 | 101 | 50 | 107 | 1.29e-25 | 103 |
| Msa0272460 | MtrunA17_Chr4g0030051 | 77.193 | 57 | 12 | 1 | 42 | 97 | 37 | 93 | 1.33e-25 | 102 |
| Msa0272460 | MtrunA17_Chr7g0237921 | 79.245 | 53 | 10 | 1 | 48 | 99 | 78 | 130 | 1.42e-25 | 103 |
| Msa0272460 | MtrunA17_Chr4g0061761 | 80.000 | 50 | 10 | 0 | 44 | 93 | 38 | 87 | 3.21e-25 | 102 |
| Msa0272460 | MtrunA17_Chr2g0308011 | 79.592 | 49 | 10 | 0 | 45 | 93 | 36 | 84 | 6.06e-25 | 99.0 |
| Msa0272460 | MtrunA17_Chr1g0177251 | 56.627 | 83 | 35 | 1 | 47 | 128 | 13 | 95 | 7.36e-25 | 100 |
| Msa0272460 | MtrunA17_Chr3g0092111 | 45.455 | 99 | 54 | 0 | 48 | 146 | 138 | 236 | 4.36e-24 | 100 |
| Msa0272460 | MtrunA17_Chr3g0117601 | 46.789 | 109 | 41 | 4 | 1 | 93 | 1 | 108 | 2.66e-23 | 97.1 |
| Msa0272460 | MtrunA17_Chr5g0411951 | 75.000 | 48 | 12 | 0 | 46 | 93 | 43 | 90 | 2.66e-23 | 97.1 |
| Msa0272460 | MtrunA17_Chr6g0460731 | 52.874 | 87 | 37 | 2 | 44 | 129 | 94 | 177 | 9.55e-23 | 96.3 |
| Msa0272460 | MtrunA17_Chr4g0042481 | 66.102 | 59 | 19 | 1 | 46 | 103 | 122 | 180 | 1.10e-22 | 97.1 |
| Msa0272460 | MtrunA17_Chr7g0217581 | 74.074 | 54 | 13 | 1 | 46 | 98 | 232 | 285 | 1.12e-22 | 97.4 |
| Msa0272460 | MtrunA17_Chr2g0282441 | 78.261 | 46 | 10 | 0 | 48 | 93 | 27 | 72 | 1.92e-22 | 94.7 |
| Msa0272460 | MtrunA17_Chr6g0460721 | 67.857 | 56 | 17 | 1 | 46 | 100 | 89 | 144 | 1.96e-22 | 94.7 |
| Msa0272460 | MtrunA17_Chr2g0283811 | 62.963 | 54 | 20 | 0 | 46 | 99 | 47 | 100 | 4.34e-22 | 90.1 |
| Msa0272460 | MtrunA17_Chr7g0254161 | 67.857 | 56 | 17 | 1 | 46 | 100 | 101 | 156 | 4.63e-22 | 94.7 |
| Msa0272460 | MtrunA17_Chr6g0453321 | 73.077 | 52 | 13 | 1 | 48 | 98 | 138 | 189 | 4.88e-22 | 95.1 |
| Msa0272460 | MtrunA17_Chr5g0417571 | 58.209 | 67 | 27 | 1 | 33 | 98 | 125 | 191 | 1.34e-21 | 92.4 |
| Msa0272460 | MtrunA17_Chr8g0355261 | 49.333 | 75 | 37 | 1 | 48 | 121 | 120 | 194 | 1.81e-21 | 93.2 |
| Msa0272460 | MtrunA17_Chr5g0417601 | 58.209 | 67 | 27 | 1 | 33 | 98 | 127 | 193 | 5.64e-20 | 88.6 |
| Msa0272460 | MtrunA17_Chr5g0417561 | 69.231 | 52 | 15 | 1 | 48 | 98 | 121 | 172 | 6.63e-20 | 88.2 |
| Msa0272460 | MtrunA17_Chr5g0417551 | 63.793 | 58 | 20 | 1 | 48 | 104 | 129 | 186 | 8.00e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0272460 | AT1G28310.1 | 65.060 | 83 | 28 | 1 | 20 | 101 | 1 | 83 | 3.64e-29 | 112 |
| Msa0272460 | AT1G28310.2 | 65.060 | 83 | 28 | 1 | 20 | 101 | 25 | 107 | 7.08e-29 | 112 |
| Msa0272460 | AT5G66940.1 | 60.274 | 73 | 27 | 1 | 42 | 112 | 28 | 100 | 9.50e-28 | 107 |
| Msa0272460 | AT5G60850.1 | 59.770 | 87 | 31 | 2 | 34 | 116 | 36 | 122 | 1.86e-27 | 108 |
| Msa0272460 | AT1G51700.1 | 38.750 | 160 | 85 | 4 | 33 | 179 | 16 | 175 | 1.98e-27 | 105 |
| Msa0272460 | AT5G60200.1 | 57.447 | 94 | 32 | 3 | 19 | 111 | 32 | 118 | 2.94e-27 | 106 |
| Msa0272460 | AT3G55370.2 | 66.667 | 66 | 21 | 1 | 45 | 109 | 75 | 140 | 2.95e-27 | 108 |
| Msa0272460 | AT3G55370.1 | 66.667 | 66 | 21 | 1 | 45 | 109 | 75 | 140 | 2.96e-27 | 108 |
| Msa0272460 | AT3G55370.3 | 66.667 | 66 | 21 | 1 | 45 | 109 | 120 | 185 | 3.32e-27 | 108 |
| Msa0272460 | AT3G61850.2 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 1.97e-26 | 105 |
| Msa0272460 | AT3G61850.3 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 1.97e-26 | 105 |
| Msa0272460 | AT2G28510.1 | 77.193 | 57 | 12 | 1 | 44 | 99 | 46 | 102 | 2.21e-26 | 105 |
| Msa0272460 | AT3G61850.1 | 75.439 | 57 | 13 | 1 | 44 | 99 | 72 | 128 | 2.38e-26 | 105 |
| Msa0272460 | AT3G61850.5 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 2.93e-26 | 105 |
| Msa0272460 | AT3G45610.1 | 48.958 | 96 | 43 | 2 | 44 | 133 | 38 | 133 | 3.26e-26 | 103 |
| Msa0272460 | AT3G61850.4 | 75.439 | 57 | 13 | 1 | 44 | 99 | 72 | 128 | 3.76e-26 | 105 |
| Msa0272460 | AT3G50410.1 | 64.789 | 71 | 18 | 2 | 30 | 99 | 20 | 84 | 4.06e-26 | 103 |
| Msa0272460 | AT2G37590.1 | 61.429 | 70 | 24 | 2 | 35 | 101 | 76 | 145 | 9.19e-26 | 104 |
| Msa0272460 | AT4G24060.1 | 73.684 | 57 | 14 | 1 | 44 | 99 | 51 | 107 | 1.42e-25 | 103 |
| Msa0272460 | AT1G64620.1 | 57.692 | 78 | 31 | 1 | 16 | 93 | 21 | 96 | 3.13e-25 | 103 |
| Msa0272460 | AT2G28810.2 | 77.551 | 49 | 11 | 0 | 45 | 93 | 76 | 124 | 3.56e-25 | 102 |
| Msa0272460 | AT1G07640.1 | 70.690 | 58 | 15 | 1 | 46 | 101 | 21 | 78 | 3.70e-25 | 101 |
| Msa0272460 | AT3G21270.1 | 79.245 | 53 | 10 | 1 | 46 | 97 | 29 | 81 | 4.82e-25 | 99.4 |
| Msa0272460 | AT2G28810.1 | 77.551 | 49 | 11 | 0 | 45 | 93 | 93 | 141 | 5.39e-25 | 102 |
| Msa0272460 | AT4G38000.1 | 50.617 | 81 | 35 | 1 | 45 | 125 | 40 | 115 | 5.47e-25 | 100 |
| Msa0272460 | AT1G07640.2 | 70.690 | 58 | 15 | 1 | 46 | 101 | 77 | 134 | 5.74e-25 | 102 |
| Msa0272460 | AT4G21050.1 | 66.129 | 62 | 20 | 1 | 48 | 108 | 26 | 87 | 6.28e-25 | 99.4 |
| Msa0272460 | AT5G02460.1 | 73.214 | 56 | 14 | 1 | 45 | 99 | 94 | 149 | 6.60e-25 | 102 |
| Msa0272460 | AT1G07640.3 | 70.690 | 58 | 15 | 1 | 46 | 101 | 85 | 142 | 6.86e-25 | 102 |
| Msa0272460 | AT1G21340.1 | 55.128 | 78 | 31 | 2 | 25 | 101 | 20 | 94 | 1.82e-24 | 99.4 |
| Msa0272460 | AT5G65590.1 | 61.111 | 72 | 24 | 2 | 29 | 99 | 27 | 95 | 2.00e-24 | 100 |
| Msa0272460 | AT2G46590.1 | 74.000 | 50 | 13 | 0 | 44 | 93 | 66 | 115 | 4.07e-24 | 100 |
| Msa0272460 | AT2G46590.2 | 74.000 | 50 | 13 | 0 | 44 | 93 | 78 | 127 | 4.70e-24 | 100 |
| Msa0272460 | AT3G52440.1 | 68.966 | 58 | 17 | 1 | 48 | 104 | 27 | 84 | 1.19e-23 | 96.7 |
| Msa0272460 | AT3G52440.2 | 68.966 | 58 | 17 | 1 | 48 | 104 | 46 | 103 | 1.75e-23 | 96.7 |
| Msa0272460 | AT4G00940.1 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.30e-23 | 95.9 |
| Msa0272460 | AT4G00940.3 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.30e-23 | 95.9 |
| Msa0272460 | AT4G00940.2 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.30e-23 | 95.9 |
| Msa0272460 | AT3G47500.1 | 76.923 | 52 | 11 | 1 | 48 | 98 | 112 | 163 | 6.42e-23 | 97.8 |
| Msa0272460 | AT5G39660.3 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 9.27e-23 | 97.4 |
| Msa0272460 | AT5G39660.1 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 9.27e-23 | 97.4 |
| Msa0272460 | AT5G39660.2 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 9.27e-23 | 97.4 |
| Msa0272460 | AT5G62430.1 | 67.742 | 62 | 15 | 2 | 48 | 108 | 56 | 113 | 1.18e-22 | 95.1 |
| Msa0272460 | AT4G21040.1 | 64.286 | 56 | 20 | 0 | 48 | 103 | 27 | 82 | 1.39e-22 | 93.6 |
| Msa0272460 | AT1G47655.1 | 70.000 | 50 | 15 | 0 | 44 | 93 | 27 | 76 | 1.49e-22 | 92.8 |
| Msa0272460 | AT1G69570.1 | 72.222 | 54 | 14 | 1 | 46 | 98 | 132 | 185 | 1.87e-22 | 95.9 |
| Msa0272460 | AT5G62940.1 | 78.261 | 46 | 10 | 0 | 48 | 93 | 75 | 120 | 3.14e-22 | 95.1 |
| Msa0272460 | AT1G26790.1 | 73.077 | 52 | 13 | 1 | 48 | 98 | 113 | 164 | 4.97e-22 | 94.7 |
| Msa0272460 | AT4G21080.1 | 51.471 | 68 | 33 | 0 | 48 | 115 | 27 | 94 | 5.77e-22 | 92.4 |
| Msa0272460 | AT2G34140.1 | 68.519 | 54 | 16 | 1 | 46 | 98 | 58 | 111 | 6.26e-22 | 90.1 |
| Msa0272460 | AT1G29160.1 | 68.519 | 54 | 16 | 1 | 46 | 98 | 62 | 115 | 7.29e-22 | 90.1 |
| Msa0272460 | AT4G21030.1 | 49.275 | 69 | 34 | 1 | 48 | 115 | 23 | 91 | 4.97e-14 | 69.3 |
Find 62 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGAGGATATTGATTCTT+TGG | 0.256542 | 2_3:+3995121 | Msa0272460:CDS |
| TTTCTCATGGGAGGAGTCTT+TGG | 0.293343 | 2_3:-3994444 | None:intergenic |
| GTTACCACCTGATTGATCTT+TGG | 0.308181 | 2_3:-3994835 | None:intergenic |
| GTTGTTGTAGTAACAAAATT+TGG | 0.316594 | 2_3:-3994529 | None:intergenic |
| GGTGGTAACAATGAAGAATT+TGG | 0.329209 | 2_3:+3994849 | Msa0272460:CDS |
| GTCATTTGAGGTGGTGGTGG+AGG | 0.372781 | 2_3:-3994721 | None:intergenic |
| TGGTGAAGAGTTTAAAATAA+TGG | 0.381474 | 2_3:+3994983 | Msa0272460:CDS |
| TCTTGCAAGCTCTGCAGAAA+TGG | 0.390626 | 2_3:-3994572 | None:intergenic |
| AACAATGAAGAATTTGGTAT+TGG | 0.395188 | 2_3:+3994855 | Msa0272460:CDS |
| TTTGATCTTGCAACAAGGTT+TGG | 0.402739 | 2_3:-3994410 | None:intergenic |
| ATTCTGCCTGTTGTCATTTG+AGG | 0.406425 | 2_3:-3994733 | None:intergenic |
| TCCATGCCACTACTTGTTGC+AGG | 0.407180 | 2_3:-3995080 | None:intergenic |
| AGAATGGGAATATAAAGCTT+TGG | 0.413450 | 2_3:-3994920 | None:intergenic |
| AAAGAGCTTGAGTTTGAGAA+TGG | 0.415643 | 2_3:-3994936 | None:intergenic |
| TCATCCAATGGATATAACTA+TGG | 0.419434 | 2_3:+3994963 | Msa0272460:CDS |
| CAACTGATCTAAATGTTCCT+TGG | 0.420889 | 2_3:+3995147 | Msa0272460:CDS |
| TTTCTAGTAGAGGTGTTGAT+TGG | 0.423681 | 2_3:+3994385 | Msa0272460:CDS |
| AACTGATCTAAATGTTCCTT+GGG | 0.432000 | 2_3:+3995148 | Msa0272460:CDS |
| ATGTTCCTGTTGGTGGTGTT+AGG | 0.438700 | 2_3:+3994625 | Msa0272460:CDS |
| CTCAGGAATGTTCCTGTTGG+TGG | 0.438781 | 2_3:+3994618 | Msa0272460:CDS |
| GGACTAAAGGTGGTACTCTC+AGG | 0.447007 | 2_3:+3994601 | Msa0272460:CDS |
| GAGGTGATGCTAATAGAAGT+TGG | 0.447374 | 2_3:-3994678 | None:intergenic |
| TTCTTCCTAACACCACCAAC+AGG | 0.450348 | 2_3:-3994630 | None:intergenic |
| TTCACCATAGTTATATCCAT+TGG | 0.450485 | 2_3:-3994967 | None:intergenic |
| GCATGGATATGTCAAACTAT+TGG | 0.455144 | 2_3:+3995096 | Msa0272460:CDS |
| ATTTGAGGTGGTGGTGGAGG+AGG | 0.457027 | 2_3:-3994718 | None:intergenic |
| GTATTTGTAGAGTCACATCT+TGG | 0.471158 | 2_3:-3994507 | None:intergenic |
| GCAGAGCTTGCAAGAGACAT+TGG | 0.471598 | 2_3:+3994580 | Msa0272460:CDS |
| GTTGTCATTTGAGGTGGTGG+TGG | 0.475259 | 2_3:-3994724 | None:intergenic |
| CCTGTTGTCATTTGAGGTGG+TGG | 0.483435 | 2_3:-3994727 | None:intergenic |
| TTGGTACTCATTAGATTCTG+TGG | 0.490071 | 2_3:-3994816 | None:intergenic |
| CCACCACCTCAAATGACAAC+AGG | 0.496169 | 2_3:+3994727 | Msa0272460:CDS |
| ATGAGTACCAAAGATCAATC+AGG | 0.500364 | 2_3:+3994828 | Msa0272460:CDS |
| TATGTCAAACTATTGGAGTT+GGG | 0.508899 | 2_3:+3995103 | Msa0272460:CDS |
| TGCAAGAGACATTGGACTAA+AGG | 0.515682 | 2_3:+3994588 | Msa0272460:CDS |
| AAGAGCTTGAGTTTGAGAAT+GGG | 0.526214 | 2_3:-3994935 | None:intergenic |
| ATATGTCAAACTATTGGAGT+TGG | 0.531877 | 2_3:+3995102 | Msa0272460:CDS |
| ATGCCAAGCACTAGTGGTAG+TGG | 0.534649 | 2_3:+3995032 | Msa0272460:CDS |
| GTGGCACTATTACACATCCA+TGG | 0.536686 | 2_3:+3995051 | Msa0272460:CDS |
| CTGCCTGTTGTCATTTGAGG+TGG | 0.547681 | 2_3:-3994730 | None:intergenic |
| TCTAAGCAAGTTTCTAGTAG+AGG | 0.557594 | 2_3:+3994375 | Msa0272460:CDS |
| AGAGATTCATGCATAGAAGA+AGG | 0.563751 | 2_3:-3994768 | None:intergenic |
| ACCTGCAACAAGTAGTGGCA+TGG | 0.573077 | 2_3:+3995079 | Msa0272460:CDS |
| TTCTGTGGTGGCAACAAAGA+TGG | 0.580745 | 2_3:-3994801 | None:intergenic |
| GTGCCACTACCACTAGTGCT+TGG | 0.582189 | 2_3:-3995035 | None:intergenic |
| CTTGTTGCAGGTATCTCCCA+TGG | 0.587087 | 2_3:-3995068 | None:intergenic |
| GAGATACCTGCAACAAGTAG+TGG | 0.600865 | 2_3:+3995074 | Msa0272460:CDS |
| ACTCTCAGGAATGTTCCTGT+TGG | 0.606251 | 2_3:+3994615 | Msa0272460:CDS |
| TGAAGAGTTTAAAATAATGG+AGG | 0.607082 | 2_3:+3994986 | Msa0272460:CDS |
| GTACTCATTAGATTCTGTGG+TGG | 0.610936 | 2_3:-3994813 | None:intergenic |
| AGTGCAATGCCAAGCACTAG+TGG | 0.615832 | 2_3:+3995026 | Msa0272460:CDS |
| TTCATGCATAGAAGAAGGTG+TGG | 0.623493 | 2_3:-3994763 | None:intergenic |
| TGGCACTATTACACATCCAT+GGG | 0.627148 | 2_3:+3995052 | Msa0272460:CDS |
| CAAGCTCTGCAGAAATGGCG+AGG | 0.631289 | 2_3:-3994567 | None:intergenic |
| AGCTCTTTAAGATCATCCAA+TGG | 0.640870 | 2_3:+3994951 | Msa0272460:CDS |
| CTTGTTGCAAGATCAAAACA+TGG | 0.647907 | 2_3:+3994416 | Msa0272460:CDS |
| AAGAGACATTGGACTAAAGG+TGG | 0.661565 | 2_3:+3994591 | Msa0272460:CDS |
| GTCAAACTATTGGAGTTGGG+AGG | 0.665707 | 2_3:+3995106 | Msa0272460:CDS |
| GCAACAAAATCAGCAACTAG+AGG | 0.669254 | 2_3:+3994473 | Msa0272460:CDS |
| TTGATATCAGAATCATCCCA+AGG | 0.669368 | 2_3:-3995164 | None:intergenic |
| AGTACCAAAGATCAATCAGG+TGG | 0.670824 | 2_3:+3994831 | Msa0272460:CDS |
| TGTGGTGGCAACAAAGATGG+TGG | 0.671597 | 2_3:-3994798 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTGTTGTAGTAACAAAATT+TGG | - | chr2_3:3994532-3994551 | None:intergenic | 25.0% |
| ! | AACAATGAAGAATTTGGTAT+TGG | + | chr2_3:3994855-3994874 | Msa0272460:CDS | 25.0% |
| !!! | AATATAAAGCTTTGGTTTTG+AGG | - | chr2_3:3994915-3994934 | None:intergenic | 25.0% |
| !! | TGGTGAAGAGTTTAAAATAA+TGG | + | chr2_3:3994983-3995002 | Msa0272460:CDS | 25.0% |
| !! | TGAAGAGTTTAAAATAATGG+AGG | + | chr2_3:3994986-3995005 | Msa0272460:CDS | 25.0% |
| ! | GTTGTTGTAGTAACAAAATT+TGG | - | chr2_3:3994532-3994551 | None:intergenic | 25.0% |
| ! | AACAATGAAGAATTTGGTAT+TGG | + | chr2_3:3994855-3994874 | Msa0272460:CDS | 25.0% |
| !!! | AATATAAAGCTTTGGTTTTG+AGG | - | chr2_3:3994915-3994934 | None:intergenic | 25.0% |
| !! | TGGTGAAGAGTTTAAAATAA+TGG | + | chr2_3:3994983-3995002 | Msa0272460:CDS | 25.0% |
| !! | TGAAGAGTTTAAAATAATGG+AGG | + | chr2_3:3994986-3995005 | Msa0272460:CDS | 25.0% |
| AGAATGGGAATATAAAGCTT+TGG | - | chr2_3:3994923-3994942 | None:intergenic | 30.0% | |
| TCATCCAATGGATATAACTA+TGG | + | chr2_3:3994963-3994982 | Msa0272460:CDS | 30.0% | |
| TTCACCATAGTTATATCCAT+TGG | - | chr2_3:3994970-3994989 | None:intergenic | 30.0% | |
| ATATGTCAAACTATTGGAGT+TGG | + | chr2_3:3995102-3995121 | Msa0272460:CDS | 30.0% | |
| TATGTCAAACTATTGGAGTT+GGG | + | chr2_3:3995103-3995122 | Msa0272460:CDS | 30.0% | |
| AACTGATCTAAATGTTCCTT+GGG | + | chr2_3:3995148-3995167 | Msa0272460:CDS | 30.0% | |
| AGAATGGGAATATAAAGCTT+TGG | - | chr2_3:3994923-3994942 | None:intergenic | 30.0% | |
| TCATCCAATGGATATAACTA+TGG | + | chr2_3:3994963-3994982 | Msa0272460:CDS | 30.0% | |
| TTCACCATAGTTATATCCAT+TGG | - | chr2_3:3994970-3994989 | None:intergenic | 30.0% | |
| ATATGTCAAACTATTGGAGT+TGG | + | chr2_3:3995102-3995121 | Msa0272460:CDS | 30.0% | |
| TATGTCAAACTATTGGAGTT+GGG | + | chr2_3:3995103-3995122 | Msa0272460:CDS | 30.0% | |
| AACTGATCTAAATGTTCCTT+GGG | + | chr2_3:3995148-3995167 | Msa0272460:CDS | 30.0% | |
| !! | TCTAAGCAAGTTTCTAGTAG+AGG | + | chr2_3:3994375-3994394 | Msa0272460:CDS | 35.0% |
| !! | TTTCTAGTAGAGGTGTTGAT+TGG | + | chr2_3:3994385-3994404 | Msa0272460:CDS | 35.0% |
| TTTGATCTTGCAACAAGGTT+TGG | - | chr2_3:3994413-3994432 | None:intergenic | 35.0% | |
| !!! | CATGTTTTGATCTTGCAACA+AGG | - | chr2_3:3994418-3994437 | None:intergenic | 35.0% |
| CTTGTTGCAAGATCAAAACA+TGG | + | chr2_3:3994416-3994435 | Msa0272460:CDS | 35.0% | |
| !!! | TGTTGCTGTTGTTTTCTCAT+GGG | - | chr2_3:3994459-3994478 | None:intergenic | 35.0% |
| !!! | TTGTTGCTGTTGTTTTCTCA+TGG | - | chr2_3:3994460-3994479 | None:intergenic | 35.0% |
| GTATTTGTAGAGTCACATCT+TGG | - | chr2_3:3994510-3994529 | None:intergenic | 35.0% | |
| ! | GGTGCTAATAGTAGTTTTTG+AGG | - | chr2_3:3994700-3994719 | None:intergenic | 35.0% |
| AGAGATTCATGCATAGAAGA+AGG | - | chr2_3:3994771-3994790 | None:intergenic | 35.0% | |
| TTGGTACTCATTAGATTCTG+TGG | - | chr2_3:3994819-3994838 | None:intergenic | 35.0% | |
| ATGAGTACCAAAGATCAATC+AGG | + | chr2_3:3994828-3994847 | Msa0272460:CDS | 35.0% | |
| GGTGGTAACAATGAAGAATT+TGG | + | chr2_3:3994849-3994868 | Msa0272460:CDS | 35.0% | |
| !!! | AAGCTTTGGTTTTGAGGAAA+AGG | - | chr2_3:3994909-3994928 | None:intergenic | 35.0% |
| AAGAGCTTGAGTTTGAGAAT+GGG | - | chr2_3:3994938-3994957 | None:intergenic | 35.0% | |
| AAAGAGCTTGAGTTTGAGAA+TGG | - | chr2_3:3994939-3994958 | None:intergenic | 35.0% | |
| !! | AGCTCTTTAAGATCATCCAA+TGG | + | chr2_3:3994951-3994970 | Msa0272460:CDS | 35.0% |
| GCATGGATATGTCAAACTAT+TGG | + | chr2_3:3995096-3995115 | Msa0272460:CDS | 35.0% | |
| !! | TTGGGAGGATATTGATTCTT+TGG | + | chr2_3:3995121-3995140 | Msa0272460:CDS | 35.0% |
| CAACTGATCTAAATGTTCCT+TGG | + | chr2_3:3995147-3995166 | Msa0272460:CDS | 35.0% | |
| TTGATATCAGAATCATCCCA+AGG | - | chr2_3:3995167-3995186 | None:intergenic | 35.0% | |
| !! | TCTAAGCAAGTTTCTAGTAG+AGG | + | chr2_3:3994375-3994394 | Msa0272460:CDS | 35.0% |
| !! | TTTCTAGTAGAGGTGTTGAT+TGG | + | chr2_3:3994385-3994404 | Msa0272460:CDS | 35.0% |
| TTTGATCTTGCAACAAGGTT+TGG | - | chr2_3:3994413-3994432 | None:intergenic | 35.0% | |
| !!! | CATGTTTTGATCTTGCAACA+AGG | - | chr2_3:3994418-3994437 | None:intergenic | 35.0% |
| CTTGTTGCAAGATCAAAACA+TGG | + | chr2_3:3994416-3994435 | Msa0272460:CDS | 35.0% | |
| !!! | TGTTGCTGTTGTTTTCTCAT+GGG | - | chr2_3:3994459-3994478 | None:intergenic | 35.0% |
| !!! | TTGTTGCTGTTGTTTTCTCA+TGG | - | chr2_3:3994460-3994479 | None:intergenic | 35.0% |
| GTATTTGTAGAGTCACATCT+TGG | - | chr2_3:3994510-3994529 | None:intergenic | 35.0% | |
| ! | GGTGCTAATAGTAGTTTTTG+AGG | - | chr2_3:3994700-3994719 | None:intergenic | 35.0% |
| AGAGATTCATGCATAGAAGA+AGG | - | chr2_3:3994771-3994790 | None:intergenic | 35.0% | |
| TTGGTACTCATTAGATTCTG+TGG | - | chr2_3:3994819-3994838 | None:intergenic | 35.0% | |
| ATGAGTACCAAAGATCAATC+AGG | + | chr2_3:3994828-3994847 | Msa0272460:CDS | 35.0% | |
| GGTGGTAACAATGAAGAATT+TGG | + | chr2_3:3994849-3994868 | Msa0272460:CDS | 35.0% | |
| !!! | AAGCTTTGGTTTTGAGGAAA+AGG | - | chr2_3:3994909-3994928 | None:intergenic | 35.0% |
| AAGAGCTTGAGTTTGAGAAT+GGG | - | chr2_3:3994938-3994957 | None:intergenic | 35.0% | |
| AAAGAGCTTGAGTTTGAGAA+TGG | - | chr2_3:3994939-3994958 | None:intergenic | 35.0% | |
| !! | AGCTCTTTAAGATCATCCAA+TGG | + | chr2_3:3994951-3994970 | Msa0272460:CDS | 35.0% |
| GCATGGATATGTCAAACTAT+TGG | + | chr2_3:3995096-3995115 | Msa0272460:CDS | 35.0% | |
| !! | TTGGGAGGATATTGATTCTT+TGG | + | chr2_3:3995121-3995140 | Msa0272460:CDS | 35.0% |
| CAACTGATCTAAATGTTCCT+TGG | + | chr2_3:3995147-3995166 | Msa0272460:CDS | 35.0% | |
| TTGATATCAGAATCATCCCA+AGG | - | chr2_3:3995167-3995186 | None:intergenic | 35.0% | |
| GCAACAAAATCAGCAACTAG+AGG | + | chr2_3:3994473-3994492 | Msa0272460:CDS | 40.0% | |
| TGCAAGAGACATTGGACTAA+AGG | + | chr2_3:3994588-3994607 | Msa0272460:CDS | 40.0% | |
| AAGAGACATTGGACTAAAGG+TGG | + | chr2_3:3994591-3994610 | Msa0272460:CDS | 40.0% | |
| GAGGTGATGCTAATAGAAGT+TGG | - | chr2_3:3994681-3994700 | None:intergenic | 40.0% | |
| ! | ATTCTGCCTGTTGTCATTTG+AGG | - | chr2_3:3994736-3994755 | None:intergenic | 40.0% |
| TTCATGCATAGAAGAAGGTG+TGG | - | chr2_3:3994766-3994785 | None:intergenic | 40.0% | |
| GTACTCATTAGATTCTGTGG+TGG | - | chr2_3:3994816-3994835 | None:intergenic | 40.0% | |
| AGTACCAAAGATCAATCAGG+TGG | + | chr2_3:3994831-3994850 | Msa0272460:CDS | 40.0% | |
| GTTACCACCTGATTGATCTT+TGG | - | chr2_3:3994838-3994857 | None:intergenic | 40.0% | |
| TGGCACTATTACACATCCAT+GGG | + | chr2_3:3995052-3995071 | Msa0272460:CDS | 40.0% | |
| GCAACAAAATCAGCAACTAG+AGG | + | chr2_3:3994473-3994492 | Msa0272460:CDS | 40.0% | |
| TGCAAGAGACATTGGACTAA+AGG | + | chr2_3:3994588-3994607 | Msa0272460:CDS | 40.0% | |
| AAGAGACATTGGACTAAAGG+TGG | + | chr2_3:3994591-3994610 | Msa0272460:CDS | 40.0% | |
| GAGGTGATGCTAATAGAAGT+TGG | - | chr2_3:3994681-3994700 | None:intergenic | 40.0% | |
| ! | ATTCTGCCTGTTGTCATTTG+AGG | - | chr2_3:3994736-3994755 | None:intergenic | 40.0% |
| TTCATGCATAGAAGAAGGTG+TGG | - | chr2_3:3994766-3994785 | None:intergenic | 40.0% | |
| GTACTCATTAGATTCTGTGG+TGG | - | chr2_3:3994816-3994835 | None:intergenic | 40.0% | |
| AGTACCAAAGATCAATCAGG+TGG | + | chr2_3:3994831-3994850 | Msa0272460:CDS | 40.0% | |
| GTTACCACCTGATTGATCTT+TGG | - | chr2_3:3994838-3994857 | None:intergenic | 40.0% | |
| TGGCACTATTACACATCCAT+GGG | + | chr2_3:3995052-3995071 | Msa0272460:CDS | 40.0% | |
| TTTCTCATGGGAGGAGTCTT+TGG | - | chr2_3:3994447-3994466 | None:intergenic | 45.0% | |
| !!! | TGCTGTTGTTTTCTCATGGG+AGG | - | chr2_3:3994456-3994475 | None:intergenic | 45.0% |
| TCTTGCAAGCTCTGCAGAAA+TGG | - | chr2_3:3994575-3994594 | None:intergenic | 45.0% | |
| ACTCTCAGGAATGTTCCTGT+TGG | + | chr2_3:3994615-3994634 | Msa0272460:CDS | 45.0% | |
| !! | ATGTTCCTGTTGGTGGTGTT+AGG | + | chr2_3:3994625-3994644 | Msa0272460:CDS | 45.0% |
| TTCTTCCTAACACCACCAAC+AGG | - | chr2_3:3994633-3994652 | None:intergenic | 45.0% | |
| TTCTGTGGTGGCAACAAAGA+TGG | - | chr2_3:3994804-3994823 | None:intergenic | 45.0% | |
| GTGGCACTATTACACATCCA+TGG | + | chr2_3:3995051-3995070 | Msa0272460:CDS | 45.0% | |
| GAGATACCTGCAACAAGTAG+TGG | + | chr2_3:3995074-3995093 | Msa0272460:CDS | 45.0% | |
| GTCAAACTATTGGAGTTGGG+AGG | + | chr2_3:3995106-3995125 | Msa0272460:CDS | 45.0% | |
| TTTCTCATGGGAGGAGTCTT+TGG | - | chr2_3:3994447-3994466 | None:intergenic | 45.0% | |
| !!! | TGCTGTTGTTTTCTCATGGG+AGG | - | chr2_3:3994456-3994475 | None:intergenic | 45.0% |
| TCTTGCAAGCTCTGCAGAAA+TGG | - | chr2_3:3994575-3994594 | None:intergenic | 45.0% | |
| ACTCTCAGGAATGTTCCTGT+TGG | + | chr2_3:3994615-3994634 | Msa0272460:CDS | 45.0% | |
| !! | ATGTTCCTGTTGGTGGTGTT+AGG | + | chr2_3:3994625-3994644 | Msa0272460:CDS | 45.0% |
| TTCTTCCTAACACCACCAAC+AGG | - | chr2_3:3994633-3994652 | None:intergenic | 45.0% | |
| TTCTGTGGTGGCAACAAAGA+TGG | - | chr2_3:3994804-3994823 | None:intergenic | 45.0% | |
| GTGGCACTATTACACATCCA+TGG | + | chr2_3:3995051-3995070 | Msa0272460:CDS | 45.0% | |
| GAGATACCTGCAACAAGTAG+TGG | + | chr2_3:3995074-3995093 | Msa0272460:CDS | 45.0% | |
| GTCAAACTATTGGAGTTGGG+AGG | + | chr2_3:3995106-3995125 | Msa0272460:CDS | 45.0% | |
| GCAGAGCTTGCAAGAGACAT+TGG | + | chr2_3:3994580-3994599 | Msa0272460:CDS | 50.0% | |
| GGACTAAAGGTGGTACTCTC+AGG | + | chr2_3:3994601-3994620 | Msa0272460:CDS | 50.0% | |
| CTCAGGAATGTTCCTGTTGG+TGG | + | chr2_3:3994618-3994637 | Msa0272460:CDS | 50.0% | |
| ! | GTTGTCATTTGAGGTGGTGG+TGG | - | chr2_3:3994727-3994746 | None:intergenic | 50.0% |
| ! | CCTGTTGTCATTTGAGGTGG+TGG | - | chr2_3:3994730-3994749 | None:intergenic | 50.0% |
| CCACCACCTCAAATGACAAC+AGG | + | chr2_3:3994727-3994746 | Msa0272460:CDS | 50.0% | |
| ! | CTGCCTGTTGTCATTTGAGG+TGG | - | chr2_3:3994733-3994752 | None:intergenic | 50.0% |
| TGTGGTGGCAACAAAGATGG+TGG | - | chr2_3:3994801-3994820 | None:intergenic | 50.0% | |
| AGTGCAATGCCAAGCACTAG+TGG | + | chr2_3:3995026-3995045 | Msa0272460:CDS | 50.0% | |
| ATGCCAAGCACTAGTGGTAG+TGG | + | chr2_3:3995032-3995051 | Msa0272460:CDS | 50.0% | |
| CTTGTTGCAGGTATCTCCCA+TGG | - | chr2_3:3995071-3995090 | None:intergenic | 50.0% | |
| ACCTGCAACAAGTAGTGGCA+TGG | + | chr2_3:3995079-3995098 | Msa0272460:CDS | 50.0% | |
| ! | TCCATGCCACTACTTGTTGC+AGG | - | chr2_3:3995083-3995102 | None:intergenic | 50.0% |
| GCAGAGCTTGCAAGAGACAT+TGG | + | chr2_3:3994580-3994599 | Msa0272460:CDS | 50.0% | |
| GGACTAAAGGTGGTACTCTC+AGG | + | chr2_3:3994601-3994620 | Msa0272460:CDS | 50.0% | |
| CTCAGGAATGTTCCTGTTGG+TGG | + | chr2_3:3994618-3994637 | Msa0272460:CDS | 50.0% | |
| ! | GTTGTCATTTGAGGTGGTGG+TGG | - | chr2_3:3994727-3994746 | None:intergenic | 50.0% |
| ! | CCTGTTGTCATTTGAGGTGG+TGG | - | chr2_3:3994730-3994749 | None:intergenic | 50.0% |
| CCACCACCTCAAATGACAAC+AGG | + | chr2_3:3994727-3994746 | Msa0272460:CDS | 50.0% | |
| ! | CTGCCTGTTGTCATTTGAGG+TGG | - | chr2_3:3994733-3994752 | None:intergenic | 50.0% |
| TGTGGTGGCAACAAAGATGG+TGG | - | chr2_3:3994801-3994820 | None:intergenic | 50.0% | |
| AGTGCAATGCCAAGCACTAG+TGG | + | chr2_3:3995026-3995045 | Msa0272460:CDS | 50.0% | |
| ATGCCAAGCACTAGTGGTAG+TGG | + | chr2_3:3995032-3995051 | Msa0272460:CDS | 50.0% | |
| CTTGTTGCAGGTATCTCCCA+TGG | - | chr2_3:3995071-3995090 | None:intergenic | 50.0% | |
| ACCTGCAACAAGTAGTGGCA+TGG | + | chr2_3:3995079-3995098 | Msa0272460:CDS | 50.0% | |
| ! | TCCATGCCACTACTTGTTGC+AGG | - | chr2_3:3995083-3995102 | None:intergenic | 50.0% |
| CAAGCTCTGCAGAAATGGCG+AGG | - | chr2_3:3994570-3994589 | None:intergenic | 55.0% | |
| !! | ATTTGAGGTGGTGGTGGAGG+AGG | - | chr2_3:3994721-3994740 | None:intergenic | 55.0% |
| !! | GTCATTTGAGGTGGTGGTGG+AGG | - | chr2_3:3994724-3994743 | None:intergenic | 55.0% |
| ! | GTGCCACTACCACTAGTGCT+TGG | - | chr2_3:3995038-3995057 | None:intergenic | 55.0% |
| CAAGCTCTGCAGAAATGGCG+AGG | - | chr2_3:3994570-3994589 | None:intergenic | 55.0% | |
| !! | ATTTGAGGTGGTGGTGGAGG+AGG | - | chr2_3:3994721-3994740 | None:intergenic | 55.0% |
| !! | GTCATTTGAGGTGGTGGTGG+AGG | - | chr2_3:3994724-3994743 | None:intergenic | 55.0% |
| ! | GTGCCACTACCACTAGTGCT+TGG | - | chr2_3:3995038-3995057 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 3994363 | 3995193 | 3994363 | ID=Msa0272460;Name=Msa0272460 |
| chr2_3 | mRNA | 3994363 | 3995193 | 3994363 | ID=Msa0272460-mRNA-1;Parent=Msa0272460;Name=Msa0272460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|276 |
| chr2_3 | exon | 3994363 | 3995193 | 3994363 | ID=Msa0272460-mRNA-1:exon:2018;Parent=Msa0272460-mRNA-1 |
| chr2_3 | CDS | 3994363 | 3995193 | 3994363 | ID=Msa0272460-mRNA-1:cds;Parent=Msa0272460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
