Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312660 | XP_003593557.1 | 91.336 | 277 | 20 | 2 | 1 | 276 | 1 | 274 | 3.48e-169 | 479 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312660 | sp|Q9FZA4|DOF14_ARATH | 65.060 | 83 | 28 | 1 | 20 | 101 | 1 | 83 | 3.77e-28 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312660 | G7IPR3 | 91.336 | 277 | 20 | 2 | 1 | 276 | 1 | 274 | 1.66e-169 | 479 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0312660 | TF | C2C2-Dof |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0272460 | Msa0312660 | 0.927125 | 1.816932e-91 | -8.615850e-47 |
| Msa0312660 | Msa1386060 | 0.925785 | 1.145273e-90 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312660 | MtrunA17_Chr2g0282051 | 91.336 | 277 | 20 | 2 | 1 | 276 | 1 | 274 | 3.20e-173 | 479 |
| Msa0312660 | MtrunA17_Chr4g0047761 | 55.290 | 293 | 96 | 10 | 12 | 276 | 35 | 320 | 1.02e-80 | 246 |
| Msa0312660 | MtrunA17_Chr3g0127201 | 66.197 | 71 | 23 | 1 | 30 | 99 | 11 | 81 | 7.80e-29 | 111 |
| Msa0312660 | MtrunA17_Chr4g0047001 | 63.415 | 82 | 29 | 1 | 48 | 128 | 57 | 138 | 1.17e-28 | 112 |
| Msa0312660 | MtrunA17_Chr2g0328021 | 46.212 | 132 | 58 | 4 | 46 | 176 | 62 | 181 | 1.43e-28 | 110 |
| Msa0312660 | MtrunA17_Chr8g0374251 | 59.036 | 83 | 31 | 2 | 39 | 120 | 63 | 143 | 1.69e-28 | 111 |
| Msa0312660 | MtrunA17_Chr8g0374111 | 56.962 | 79 | 30 | 2 | 44 | 121 | 27 | 102 | 5.65e-27 | 104 |
| Msa0312660 | MtrunA17_Chr7g0223311 | 67.568 | 74 | 19 | 2 | 38 | 106 | 69 | 142 | 6.91e-27 | 107 |
| Msa0312660 | MtrunA17_Chr8g0346551 | 58.108 | 74 | 30 | 1 | 28 | 100 | 6 | 79 | 1.03e-26 | 105 |
| Msa0312660 | MtrunA17_Chr2g0326001 | 77.193 | 57 | 12 | 1 | 44 | 99 | 38 | 94 | 1.65e-26 | 105 |
| Msa0312660 | MtrunA17_Chr2g0282511 | 83.673 | 49 | 8 | 0 | 45 | 93 | 44 | 92 | 1.92e-26 | 105 |
| Msa0312660 | MtrunA17_Chr4g0031411 | 55.682 | 88 | 33 | 2 | 45 | 127 | 74 | 160 | 2.04e-26 | 105 |
| Msa0312660 | MtrunA17_Chr8g0367161 | 60.870 | 69 | 26 | 1 | 40 | 107 | 26 | 94 | 2.14e-26 | 105 |
| Msa0312660 | MtrunA17_Chr8g0341671 | 60.811 | 74 | 22 | 1 | 20 | 93 | 1 | 67 | 4.35e-26 | 102 |
| Msa0312660 | MtrunA17_Chr4g0010151 | 53.846 | 91 | 34 | 2 | 45 | 127 | 81 | 171 | 4.39e-26 | 105 |
| Msa0312660 | MtrunA17_Chr1g0188851 | 59.420 | 69 | 27 | 1 | 32 | 99 | 29 | 97 | 5.33e-26 | 103 |
| Msa0312660 | MtrunA17_Chr3g0126201 | 74.138 | 58 | 14 | 1 | 45 | 101 | 50 | 107 | 1.42e-25 | 103 |
| Msa0312660 | MtrunA17_Chr4g0030051 | 77.193 | 57 | 12 | 1 | 42 | 97 | 37 | 93 | 1.44e-25 | 102 |
| Msa0312660 | MtrunA17_Chr7g0237921 | 79.245 | 53 | 10 | 1 | 48 | 99 | 78 | 130 | 1.52e-25 | 103 |
| Msa0312660 | MtrunA17_Chr4g0061761 | 80.000 | 50 | 10 | 0 | 44 | 93 | 38 | 87 | 3.55e-25 | 102 |
| Msa0312660 | MtrunA17_Chr2g0308011 | 79.592 | 49 | 10 | 0 | 45 | 93 | 36 | 84 | 7.24e-25 | 99.0 |
| Msa0312660 | MtrunA17_Chr1g0177251 | 56.627 | 83 | 35 | 1 | 47 | 128 | 13 | 95 | 8.16e-25 | 100 |
| Msa0312660 | MtrunA17_Chr3g0092111 | 45.455 | 99 | 54 | 0 | 48 | 146 | 138 | 236 | 5.30e-24 | 100 |
| Msa0312660 | MtrunA17_Chr3g0117601 | 46.789 | 109 | 41 | 4 | 1 | 93 | 1 | 108 | 2.58e-23 | 97.4 |
| Msa0312660 | MtrunA17_Chr5g0411951 | 75.000 | 48 | 12 | 0 | 46 | 93 | 43 | 90 | 3.13e-23 | 97.1 |
| Msa0312660 | MtrunA17_Chr6g0460731 | 52.874 | 87 | 37 | 2 | 44 | 129 | 94 | 177 | 1.13e-22 | 95.9 |
| Msa0312660 | MtrunA17_Chr7g0217581 | 74.074 | 54 | 13 | 1 | 46 | 98 | 232 | 285 | 1.24e-22 | 97.1 |
| Msa0312660 | MtrunA17_Chr4g0042481 | 66.102 | 59 | 19 | 1 | 46 | 103 | 122 | 180 | 1.24e-22 | 96.7 |
| Msa0312660 | MtrunA17_Chr2g0282441 | 78.261 | 46 | 10 | 0 | 48 | 93 | 27 | 72 | 2.14e-22 | 94.7 |
| Msa0312660 | MtrunA17_Chr6g0460721 | 67.857 | 56 | 17 | 1 | 46 | 100 | 89 | 144 | 2.30e-22 | 94.4 |
| Msa0312660 | MtrunA17_Chr2g0283811 | 62.963 | 54 | 20 | 0 | 46 | 99 | 47 | 100 | 4.77e-22 | 89.7 |
| Msa0312660 | MtrunA17_Chr7g0254161 | 67.857 | 56 | 17 | 1 | 46 | 100 | 101 | 156 | 6.03e-22 | 94.4 |
| Msa0312660 | MtrunA17_Chr6g0453321 | 73.077 | 52 | 13 | 1 | 48 | 98 | 138 | 189 | 6.52e-22 | 94.7 |
| Msa0312660 | MtrunA17_Chr5g0417571 | 58.209 | 67 | 27 | 1 | 33 | 98 | 125 | 191 | 1.52e-21 | 92.0 |
| Msa0312660 | MtrunA17_Chr8g0355261 | 49.333 | 75 | 37 | 1 | 48 | 121 | 120 | 194 | 2.26e-21 | 92.8 |
| Msa0312660 | MtrunA17_Chr5g0417601 | 58.209 | 67 | 27 | 1 | 33 | 98 | 127 | 193 | 5.92e-20 | 88.6 |
| Msa0312660 | MtrunA17_Chr5g0417561 | 69.231 | 52 | 15 | 1 | 48 | 98 | 121 | 172 | 7.61e-20 | 87.8 |
| Msa0312660 | MtrunA17_Chr5g0417551 | 63.793 | 58 | 20 | 1 | 48 | 104 | 129 | 186 | 8.83e-20 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312660 | AT1G28310.1 | 65.060 | 83 | 28 | 1 | 20 | 101 | 1 | 83 | 3.84e-29 | 112 |
| Msa0312660 | AT1G28310.2 | 65.060 | 83 | 28 | 1 | 20 | 101 | 25 | 107 | 7.45e-29 | 112 |
| Msa0312660 | AT5G66940.1 | 60.274 | 73 | 27 | 1 | 42 | 112 | 28 | 100 | 1.07e-27 | 107 |
| Msa0312660 | AT5G60850.1 | 59.770 | 87 | 31 | 2 | 34 | 116 | 36 | 122 | 1.90e-27 | 108 |
| Msa0312660 | AT1G51700.1 | 38.750 | 160 | 85 | 4 | 33 | 179 | 16 | 175 | 1.95e-27 | 105 |
| Msa0312660 | AT5G60200.1 | 57.447 | 94 | 32 | 3 | 19 | 111 | 32 | 118 | 2.97e-27 | 106 |
| Msa0312660 | AT3G55370.1 | 66.667 | 66 | 21 | 1 | 45 | 109 | 75 | 140 | 3.32e-27 | 107 |
| Msa0312660 | AT3G55370.2 | 66.667 | 66 | 21 | 1 | 45 | 109 | 75 | 140 | 3.48e-27 | 108 |
| Msa0312660 | AT3G55370.3 | 66.667 | 66 | 21 | 1 | 45 | 109 | 120 | 185 | 3.83e-27 | 108 |
| Msa0312660 | AT3G61850.2 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 2.23e-26 | 104 |
| Msa0312660 | AT3G61850.3 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 2.23e-26 | 104 |
| Msa0312660 | AT2G28510.1 | 77.193 | 57 | 12 | 1 | 44 | 99 | 46 | 102 | 2.64e-26 | 104 |
| Msa0312660 | AT3G61850.1 | 75.439 | 57 | 13 | 1 | 44 | 99 | 72 | 128 | 2.73e-26 | 105 |
| Msa0312660 | AT3G61850.5 | 75.439 | 57 | 13 | 1 | 44 | 99 | 60 | 116 | 3.35e-26 | 105 |
| Msa0312660 | AT3G45610.1 | 44.538 | 119 | 53 | 3 | 21 | 133 | 22 | 133 | 3.93e-26 | 103 |
| Msa0312660 | AT3G61850.4 | 75.439 | 57 | 13 | 1 | 44 | 99 | 72 | 128 | 4.04e-26 | 105 |
| Msa0312660 | AT3G50410.1 | 64.789 | 71 | 18 | 2 | 30 | 99 | 20 | 84 | 4.60e-26 | 103 |
| Msa0312660 | AT2G37590.1 | 61.429 | 70 | 24 | 2 | 35 | 101 | 76 | 145 | 1.07e-25 | 103 |
| Msa0312660 | AT4G24060.1 | 73.684 | 57 | 14 | 1 | 44 | 99 | 51 | 107 | 1.62e-25 | 103 |
| Msa0312660 | AT1G64620.1 | 57.692 | 78 | 31 | 1 | 16 | 93 | 21 | 96 | 3.72e-25 | 102 |
| Msa0312660 | AT2G28810.2 | 77.551 | 49 | 11 | 0 | 45 | 93 | 76 | 124 | 4.10e-25 | 102 |
| Msa0312660 | AT1G07640.1 | 70.690 | 58 | 15 | 1 | 46 | 101 | 21 | 78 | 4.15e-25 | 101 |
| Msa0312660 | AT3G21270.1 | 79.245 | 53 | 10 | 1 | 46 | 97 | 29 | 81 | 5.58e-25 | 99.4 |
| Msa0312660 | AT4G38000.1 | 50.617 | 81 | 35 | 1 | 45 | 125 | 40 | 115 | 5.76e-25 | 100 |
| Msa0312660 | AT2G28810.1 | 77.551 | 49 | 11 | 0 | 45 | 93 | 93 | 141 | 5.91e-25 | 102 |
| Msa0312660 | AT1G07640.2 | 70.690 | 58 | 15 | 1 | 46 | 101 | 77 | 134 | 6.55e-25 | 102 |
| Msa0312660 | AT5G02460.1 | 73.214 | 56 | 14 | 1 | 45 | 99 | 94 | 149 | 7.52e-25 | 102 |
| Msa0312660 | AT4G21050.1 | 66.129 | 62 | 20 | 1 | 48 | 108 | 26 | 87 | 7.83e-25 | 99.0 |
| Msa0312660 | AT1G07640.3 | 70.690 | 58 | 15 | 1 | 46 | 101 | 85 | 142 | 7.98e-25 | 101 |
| Msa0312660 | AT1G21340.1 | 55.128 | 78 | 31 | 2 | 25 | 101 | 20 | 94 | 1.94e-24 | 99.4 |
| Msa0312660 | AT5G65590.1 | 61.111 | 72 | 24 | 2 | 29 | 99 | 27 | 95 | 2.00e-24 | 100 |
| Msa0312660 | AT2G46590.1 | 74.000 | 50 | 13 | 0 | 44 | 93 | 66 | 115 | 4.54e-24 | 100 |
| Msa0312660 | AT2G46590.2 | 74.000 | 50 | 13 | 0 | 44 | 93 | 78 | 127 | 5.20e-24 | 100 |
| Msa0312660 | AT3G52440.1 | 68.966 | 58 | 17 | 1 | 48 | 104 | 27 | 84 | 1.29e-23 | 96.7 |
| Msa0312660 | AT3G52440.2 | 68.966 | 58 | 17 | 1 | 48 | 104 | 46 | 103 | 1.93e-23 | 96.7 |
| Msa0312660 | AT4G00940.1 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.84e-23 | 95.9 |
| Msa0312660 | AT4G00940.3 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.84e-23 | 95.9 |
| Msa0312660 | AT4G00940.2 | 67.241 | 58 | 18 | 1 | 44 | 100 | 66 | 123 | 6.84e-23 | 95.9 |
| Msa0312660 | AT3G47500.1 | 76.923 | 52 | 11 | 1 | 48 | 98 | 112 | 163 | 7.44e-23 | 97.8 |
| Msa0312660 | AT5G39660.3 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 1.15e-22 | 97.1 |
| Msa0312660 | AT5G39660.1 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 1.15e-22 | 97.1 |
| Msa0312660 | AT5G39660.2 | 53.947 | 76 | 35 | 0 | 48 | 123 | 140 | 215 | 1.15e-22 | 97.1 |
| Msa0312660 | AT5G62430.1 | 67.742 | 62 | 15 | 2 | 48 | 108 | 56 | 113 | 1.40e-22 | 95.1 |
| Msa0312660 | AT1G47655.1 | 70.000 | 50 | 15 | 0 | 44 | 93 | 27 | 76 | 1.60e-22 | 92.8 |
| Msa0312660 | AT4G21040.1 | 64.286 | 56 | 20 | 0 | 48 | 103 | 27 | 82 | 1.62e-22 | 93.6 |
| Msa0312660 | AT1G69570.1 | 72.222 | 54 | 14 | 1 | 46 | 98 | 132 | 185 | 2.13e-22 | 95.9 |
| Msa0312660 | AT5G62940.1 | 78.261 | 46 | 10 | 0 | 48 | 93 | 75 | 120 | 3.44e-22 | 95.1 |
| Msa0312660 | AT1G26790.1 | 73.077 | 52 | 13 | 1 | 48 | 98 | 113 | 164 | 5.77e-22 | 94.4 |
| Msa0312660 | AT4G21080.1 | 51.471 | 68 | 33 | 0 | 48 | 115 | 27 | 94 | 6.14e-22 | 92.4 |
| Msa0312660 | AT2G34140.1 | 68.519 | 54 | 16 | 1 | 46 | 98 | 58 | 111 | 6.95e-22 | 90.1 |
| Msa0312660 | AT1G29160.1 | 68.519 | 54 | 16 | 1 | 46 | 98 | 62 | 115 | 8.80e-22 | 90.1 |
| Msa0312660 | AT4G21030.1 | 49.275 | 69 | 34 | 1 | 48 | 115 | 23 | 91 | 4.92e-14 | 69.3 |
Find 61 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGAGGATATTGATTCTT+TGG | 0.256542 | 2_4:-7832245 | Msa0312660:CDS |
| TTTCTCATGGGAGGAGTCTT+TGG | 0.293343 | 2_4:+7832922 | None:intergenic |
| GTTACCACCTGATTGATCTT+TGG | 0.308181 | 2_4:+7832531 | None:intergenic |
| GTTGTTGTAGTAACAAAATT+TGG | 0.316594 | 2_4:+7832837 | None:intergenic |
| GGTGGTAACAATGAAGAATT+TGG | 0.329209 | 2_4:-7832517 | Msa0312660:CDS |
| GTCATTTGAGGTGGTGGTGG+AGG | 0.372781 | 2_4:+7832645 | None:intergenic |
| TCCATGCCATTACTTGTTGC+AGG | 0.373072 | 2_4:+7832286 | None:intergenic |
| TGGTGAAGAGTTTAAAATAA+TGG | 0.381474 | 2_4:-7832383 | Msa0312660:CDS |
| TCTTGCAAGCTCTGCAGAAA+TGG | 0.390626 | 2_4:+7832794 | None:intergenic |
| AACAATGAAGAATTTGGTAT+TGG | 0.395188 | 2_4:-7832511 | Msa0312660:CDS |
| TTTGATCTTGCAACAAGGTT+TGG | 0.402739 | 2_4:+7832956 | None:intergenic |
| ATTCTGCCTGTTGTCATTTG+AGG | 0.406425 | 2_4:+7832633 | None:intergenic |
| GCATGGATATGTCAAACTAT+TGG | 0.406618 | 2_4:-7832270 | Msa0312660:CDS |
| AGAATGGGAATATAAAGCTT+TGG | 0.413450 | 2_4:+7832446 | None:intergenic |
| TCATCCAATGGATATAACTA+TGG | 0.419434 | 2_4:-7832403 | Msa0312660:CDS |
| CAACTGATCTAAATGTTCCT+TGG | 0.420889 | 2_4:-7832219 | Msa0312660:CDS |
| AAAGAGCTTGAGTTTGAGAA+TGG | 0.422009 | 2_4:+7832430 | None:intergenic |
| TTTCTAGTAGAGGTGTTGAT+TGG | 0.423681 | 2_4:-7832981 | Msa0312660:CDS |
| AACTGATCTAAATGTTCCTT+GGG | 0.432000 | 2_4:-7832218 | Msa0312660:CDS |
| ATGTTCCTGTTGGTGGTGTT+AGG | 0.438700 | 2_4:-7832741 | Msa0312660:CDS |
| CTCAGGAATGTTCCTGTTGG+TGG | 0.438781 | 2_4:-7832748 | Msa0312660:CDS |
| GGACTAAAGGTGGTACTCTC+AGG | 0.447007 | 2_4:-7832765 | Msa0312660:CDS |
| GAGGTGATGCTAATAGAAGT+TGG | 0.447374 | 2_4:+7832688 | None:intergenic |
| TTCTTCCTAACACCACCAAC+AGG | 0.450348 | 2_4:+7832736 | None:intergenic |
| TTCACCATAGTTATATCCAT+TGG | 0.450485 | 2_4:+7832399 | None:intergenic |
| ATTTGAGGTGGTGGTGGAGG+AGG | 0.457027 | 2_4:+7832648 | None:intergenic |
| GTATTTGTAGAGTCACATCT+TGG | 0.471158 | 2_4:+7832859 | None:intergenic |
| GCAGAGCTTGCAAGAGACAT+TGG | 0.471598 | 2_4:-7832786 | Msa0312660:CDS |
| GTTGTCATTTGAGGTGGTGG+TGG | 0.475259 | 2_4:+7832642 | None:intergenic |
| CCTGTTGTCATTTGAGGTGG+TGG | 0.483435 | 2_4:+7832639 | None:intergenic |
| TTGGTACTCATTAGATTCTG+TGG | 0.490071 | 2_4:+7832550 | None:intergenic |
| CCACCACCTCAAATGACAAC+AGG | 0.496169 | 2_4:-7832639 | Msa0312660:CDS |
| ATGAGTACCAAAGATCAATC+AGG | 0.500364 | 2_4:-7832538 | Msa0312660:CDS |
| TATGTCAAACTATTGGAGTT+GGG | 0.508899 | 2_4:-7832263 | Msa0312660:CDS |
| TGCAAGAGACATTGGACTAA+AGG | 0.515682 | 2_4:-7832778 | Msa0312660:CDS |
| ATATGTCAAACTATTGGAGT+TGG | 0.531877 | 2_4:-7832264 | Msa0312660:CDS |
| ATGCCAAGCACTAGTGGTAG+TGG | 0.534649 | 2_4:-7832334 | Msa0312660:CDS |
| AAGAGCTTGAGTTTGAGAAT+GGG | 0.535815 | 2_4:+7832431 | None:intergenic |
| GTGGCACTATTACACATCCA+TGG | 0.536686 | 2_4:-7832315 | Msa0312660:CDS |
| CTTGTTGCAGGTATCTCCCA+TGG | 0.540089 | 2_4:+7832298 | None:intergenic |
| CTGCCTGTTGTCATTTGAGG+TGG | 0.547681 | 2_4:+7832636 | None:intergenic |
| TCTAAGCAAGTTTCTAGTAG+AGG | 0.557594 | 2_4:-7832991 | Msa0312660:CDS |
| AGAGATTCATGCATAGAAGA+AGG | 0.563751 | 2_4:+7832598 | None:intergenic |
| ACCTGCAACAAGTAATGGCA+TGG | 0.564742 | 2_4:-7832287 | Msa0312660:CDS |
| GAGATACCTGCAACAAGTAA+TGG | 0.570653 | 2_4:-7832292 | Msa0312660:CDS |
| TTCTGTGGTGGCAACAAAGA+TGG | 0.580745 | 2_4:+7832565 | None:intergenic |
| GTGCCACTACCACTAGTGCT+TGG | 0.582189 | 2_4:+7832331 | None:intergenic |
| ACTCTCAGGAATGTTCCTGT+TGG | 0.606251 | 2_4:-7832751 | Msa0312660:CDS |
| TGAAGAGTTTAAAATAATGG+AGG | 0.607082 | 2_4:-7832380 | Msa0312660:CDS |
| GTACTCATTAGATTCTGTGG+TGG | 0.610936 | 2_4:+7832553 | None:intergenic |
| AGTGCAATGCCAAGCACTAG+TGG | 0.615832 | 2_4:-7832340 | Msa0312660:CDS |
| TTCATGCATAGAAGAAGGTG+TGG | 0.623493 | 2_4:+7832603 | None:intergenic |
| TGGCACTATTACACATCCAT+GGG | 0.627148 | 2_4:-7832314 | Msa0312660:CDS |
| CAAGCTCTGCAGAAATGGCG+AGG | 0.631289 | 2_4:+7832799 | None:intergenic |
| CTTGTTGCAAGATCAAAACA+TGG | 0.647907 | 2_4:-7832950 | Msa0312660:CDS |
| AAGAGACATTGGACTAAAGG+TGG | 0.661565 | 2_4:-7832775 | Msa0312660:CDS |
| GTCAAACTATTGGAGTTGGG+AGG | 0.665707 | 2_4:-7832260 | Msa0312660:CDS |
| GCAACAAAATCAGCAACTAG+AGG | 0.669254 | 2_4:-7832893 | Msa0312660:CDS |
| TTGATATCAGAATCATCCCA+AGG | 0.669368 | 2_4:+7832202 | None:intergenic |
| AGTACCAAAGATCAATCAGG+TGG | 0.670824 | 2_4:-7832535 | Msa0312660:CDS |
| TGTGGTGGCAACAAAGATGG+TGG | 0.671597 | 2_4:+7832568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTTGTTGTAGTAACAAAATT+TGG | + | chr2_4:7832364-7832383 | None:intergenic | 25.0% |
| ! | AACAATGAAGAATTTGGTAT+TGG | - | chr2_4:7832687-7832706 | Msa0312660:CDS | 25.0% |
| !!! | AATATAAAGCTTTGGTTTTG+AGG | + | chr2_4:7832747-7832766 | None:intergenic | 25.0% |
| !! | TGGTGAAGAGTTTAAAATAA+TGG | - | chr2_4:7832815-7832834 | Msa0312660:CDS | 25.0% |
| !! | TGAAGAGTTTAAAATAATGG+AGG | - | chr2_4:7832818-7832837 | Msa0312660:CDS | 25.0% |
| ! | GTTGTTGTAGTAACAAAATT+TGG | + | chr2_4:7832364-7832383 | None:intergenic | 25.0% |
| ! | AACAATGAAGAATTTGGTAT+TGG | - | chr2_4:7832687-7832706 | Msa0312660:CDS | 25.0% |
| !!! | AATATAAAGCTTTGGTTTTG+AGG | + | chr2_4:7832747-7832766 | None:intergenic | 25.0% |
| !! | TGGTGAAGAGTTTAAAATAA+TGG | - | chr2_4:7832815-7832834 | Msa0312660:CDS | 25.0% |
| !! | TGAAGAGTTTAAAATAATGG+AGG | - | chr2_4:7832818-7832837 | Msa0312660:CDS | 25.0% |
| AGAATGGGAATATAAAGCTT+TGG | + | chr2_4:7832755-7832774 | None:intergenic | 30.0% | |
| TCATCCAATGGATATAACTA+TGG | - | chr2_4:7832795-7832814 | Msa0312660:CDS | 30.0% | |
| TTCACCATAGTTATATCCAT+TGG | + | chr2_4:7832802-7832821 | None:intergenic | 30.0% | |
| ATATGTCAAACTATTGGAGT+TGG | - | chr2_4:7832934-7832953 | Msa0312660:CDS | 30.0% | |
| TATGTCAAACTATTGGAGTT+GGG | - | chr2_4:7832935-7832954 | Msa0312660:CDS | 30.0% | |
| AACTGATCTAAATGTTCCTT+GGG | - | chr2_4:7832980-7832999 | Msa0312660:CDS | 30.0% | |
| AGAATGGGAATATAAAGCTT+TGG | + | chr2_4:7832755-7832774 | None:intergenic | 30.0% | |
| TCATCCAATGGATATAACTA+TGG | - | chr2_4:7832795-7832814 | Msa0312660:CDS | 30.0% | |
| TTCACCATAGTTATATCCAT+TGG | + | chr2_4:7832802-7832821 | None:intergenic | 30.0% | |
| ATATGTCAAACTATTGGAGT+TGG | - | chr2_4:7832934-7832953 | Msa0312660:CDS | 30.0% | |
| TATGTCAAACTATTGGAGTT+GGG | - | chr2_4:7832935-7832954 | Msa0312660:CDS | 30.0% | |
| AACTGATCTAAATGTTCCTT+GGG | - | chr2_4:7832980-7832999 | Msa0312660:CDS | 30.0% | |
| !! | TCTAAGCAAGTTTCTAGTAG+AGG | - | chr2_4:7832207-7832226 | Msa0312660:CDS | 35.0% |
| !! | TTTCTAGTAGAGGTGTTGAT+TGG | - | chr2_4:7832217-7832236 | Msa0312660:CDS | 35.0% |
| TTTGATCTTGCAACAAGGTT+TGG | + | chr2_4:7832245-7832264 | None:intergenic | 35.0% | |
| !!! | CATGTTTTGATCTTGCAACA+AGG | + | chr2_4:7832250-7832269 | None:intergenic | 35.0% |
| CTTGTTGCAAGATCAAAACA+TGG | - | chr2_4:7832248-7832267 | Msa0312660:CDS | 35.0% | |
| !!! | TGTTGCTGTTGTTTTCTCAT+GGG | + | chr2_4:7832291-7832310 | None:intergenic | 35.0% |
| !!! | TTGTTGCTGTTGTTTTCTCA+TGG | + | chr2_4:7832292-7832311 | None:intergenic | 35.0% |
| GTATTTGTAGAGTCACATCT+TGG | + | chr2_4:7832342-7832361 | None:intergenic | 35.0% | |
| ! | GGTGCTAATAGTAGTTTTTG+AGG | + | chr2_4:7832532-7832551 | None:intergenic | 35.0% |
| AGAGATTCATGCATAGAAGA+AGG | + | chr2_4:7832603-7832622 | None:intergenic | 35.0% | |
| TTGGTACTCATTAGATTCTG+TGG | + | chr2_4:7832651-7832670 | None:intergenic | 35.0% | |
| ATGAGTACCAAAGATCAATC+AGG | - | chr2_4:7832660-7832679 | Msa0312660:CDS | 35.0% | |
| GGTGGTAACAATGAAGAATT+TGG | - | chr2_4:7832681-7832700 | Msa0312660:CDS | 35.0% | |
| !!! | AAGCTTTGGTTTTGAGGAAA+AGG | + | chr2_4:7832741-7832760 | None:intergenic | 35.0% |
| AAGAGCTTGAGTTTGAGAAT+GGG | + | chr2_4:7832770-7832789 | None:intergenic | 35.0% | |
| AAAGAGCTTGAGTTTGAGAA+TGG | + | chr2_4:7832771-7832790 | None:intergenic | 35.0% | |
| ! | AGCTCTTTTAGATCATCCAA+TGG | - | chr2_4:7832783-7832802 | Msa0312660:CDS | 35.0% |
| GCATGGATATGTCAAACTAT+TGG | - | chr2_4:7832928-7832947 | Msa0312660:CDS | 35.0% | |
| !! | TTGGGAGGATATTGATTCTT+TGG | - | chr2_4:7832953-7832972 | Msa0312660:CDS | 35.0% |
| CAACTGATCTAAATGTTCCT+TGG | - | chr2_4:7832979-7832998 | Msa0312660:CDS | 35.0% | |
| TTGATATCAGAATCATCCCA+AGG | + | chr2_4:7832999-7833018 | None:intergenic | 35.0% | |
| !! | TCTAAGCAAGTTTCTAGTAG+AGG | - | chr2_4:7832207-7832226 | Msa0312660:CDS | 35.0% |
| !! | TTTCTAGTAGAGGTGTTGAT+TGG | - | chr2_4:7832217-7832236 | Msa0312660:CDS | 35.0% |
| TTTGATCTTGCAACAAGGTT+TGG | + | chr2_4:7832245-7832264 | None:intergenic | 35.0% | |
| !!! | CATGTTTTGATCTTGCAACA+AGG | + | chr2_4:7832250-7832269 | None:intergenic | 35.0% |
| CTTGTTGCAAGATCAAAACA+TGG | - | chr2_4:7832248-7832267 | Msa0312660:CDS | 35.0% | |
| !!! | TGTTGCTGTTGTTTTCTCAT+GGG | + | chr2_4:7832291-7832310 | None:intergenic | 35.0% |
| !!! | TTGTTGCTGTTGTTTTCTCA+TGG | + | chr2_4:7832292-7832311 | None:intergenic | 35.0% |
| GTATTTGTAGAGTCACATCT+TGG | + | chr2_4:7832342-7832361 | None:intergenic | 35.0% | |
| ! | GGTGCTAATAGTAGTTTTTG+AGG | + | chr2_4:7832532-7832551 | None:intergenic | 35.0% |
| AGAGATTCATGCATAGAAGA+AGG | + | chr2_4:7832603-7832622 | None:intergenic | 35.0% | |
| TTGGTACTCATTAGATTCTG+TGG | + | chr2_4:7832651-7832670 | None:intergenic | 35.0% | |
| ATGAGTACCAAAGATCAATC+AGG | - | chr2_4:7832660-7832679 | Msa0312660:CDS | 35.0% | |
| GGTGGTAACAATGAAGAATT+TGG | - | chr2_4:7832681-7832700 | Msa0312660:CDS | 35.0% | |
| !!! | AAGCTTTGGTTTTGAGGAAA+AGG | + | chr2_4:7832741-7832760 | None:intergenic | 35.0% |
| AAGAGCTTGAGTTTGAGAAT+GGG | + | chr2_4:7832770-7832789 | None:intergenic | 35.0% | |
| AAAGAGCTTGAGTTTGAGAA+TGG | + | chr2_4:7832771-7832790 | None:intergenic | 35.0% | |
| ! | AGCTCTTTTAGATCATCCAA+TGG | - | chr2_4:7832783-7832802 | Msa0312660:CDS | 35.0% |
| GCATGGATATGTCAAACTAT+TGG | - | chr2_4:7832928-7832947 | Msa0312660:CDS | 35.0% | |
| !! | TTGGGAGGATATTGATTCTT+TGG | - | chr2_4:7832953-7832972 | Msa0312660:CDS | 35.0% |
| CAACTGATCTAAATGTTCCT+TGG | - | chr2_4:7832979-7832998 | Msa0312660:CDS | 35.0% | |
| TTGATATCAGAATCATCCCA+AGG | + | chr2_4:7832999-7833018 | None:intergenic | 35.0% | |
| GCAACAAAATCAGCAACTAG+AGG | - | chr2_4:7832305-7832324 | Msa0312660:CDS | 40.0% | |
| TGCAAGAGACATTGGACTAA+AGG | - | chr2_4:7832420-7832439 | Msa0312660:CDS | 40.0% | |
| AAGAGACATTGGACTAAAGG+TGG | - | chr2_4:7832423-7832442 | Msa0312660:CDS | 40.0% | |
| GAGGTGATGCTAATAGAAGT+TGG | + | chr2_4:7832513-7832532 | None:intergenic | 40.0% | |
| ! | ATTCTGCCTGTTGTCATTTG+AGG | + | chr2_4:7832568-7832587 | None:intergenic | 40.0% |
| TTCATGCATAGAAGAAGGTG+TGG | + | chr2_4:7832598-7832617 | None:intergenic | 40.0% | |
| GTACTCATTAGATTCTGTGG+TGG | + | chr2_4:7832648-7832667 | None:intergenic | 40.0% | |
| AGTACCAAAGATCAATCAGG+TGG | - | chr2_4:7832663-7832682 | Msa0312660:CDS | 40.0% | |
| GTTACCACCTGATTGATCTT+TGG | + | chr2_4:7832670-7832689 | None:intergenic | 40.0% | |
| TGGCACTATTACACATCCAT+GGG | - | chr2_4:7832884-7832903 | Msa0312660:CDS | 40.0% | |
| GAGATACCTGCAACAAGTAA+TGG | - | chr2_4:7832906-7832925 | Msa0312660:CDS | 40.0% | |
| GCAACAAAATCAGCAACTAG+AGG | - | chr2_4:7832305-7832324 | Msa0312660:CDS | 40.0% | |
| TGCAAGAGACATTGGACTAA+AGG | - | chr2_4:7832420-7832439 | Msa0312660:CDS | 40.0% | |
| AAGAGACATTGGACTAAAGG+TGG | - | chr2_4:7832423-7832442 | Msa0312660:CDS | 40.0% | |
| GAGGTGATGCTAATAGAAGT+TGG | + | chr2_4:7832513-7832532 | None:intergenic | 40.0% | |
| ! | ATTCTGCCTGTTGTCATTTG+AGG | + | chr2_4:7832568-7832587 | None:intergenic | 40.0% |
| TTCATGCATAGAAGAAGGTG+TGG | + | chr2_4:7832598-7832617 | None:intergenic | 40.0% | |
| GTACTCATTAGATTCTGTGG+TGG | + | chr2_4:7832648-7832667 | None:intergenic | 40.0% | |
| AGTACCAAAGATCAATCAGG+TGG | - | chr2_4:7832663-7832682 | Msa0312660:CDS | 40.0% | |
| GTTACCACCTGATTGATCTT+TGG | + | chr2_4:7832670-7832689 | None:intergenic | 40.0% | |
| TGGCACTATTACACATCCAT+GGG | - | chr2_4:7832884-7832903 | Msa0312660:CDS | 40.0% | |
| GAGATACCTGCAACAAGTAA+TGG | - | chr2_4:7832906-7832925 | Msa0312660:CDS | 40.0% | |
| TTTCTCATGGGAGGAGTCTT+TGG | + | chr2_4:7832279-7832298 | None:intergenic | 45.0% | |
| !!! | TGCTGTTGTTTTCTCATGGG+AGG | + | chr2_4:7832288-7832307 | None:intergenic | 45.0% |
| TCTTGCAAGCTCTGCAGAAA+TGG | + | chr2_4:7832407-7832426 | None:intergenic | 45.0% | |
| ACTCTCAGGAATGTTCCTGT+TGG | - | chr2_4:7832447-7832466 | Msa0312660:CDS | 45.0% | |
| !! | ATGTTCCTGTTGGTGGTGTT+AGG | - | chr2_4:7832457-7832476 | Msa0312660:CDS | 45.0% |
| TTCTTCCTAACACCACCAAC+AGG | + | chr2_4:7832465-7832484 | None:intergenic | 45.0% | |
| TTCTGTGGTGGCAACAAAGA+TGG | + | chr2_4:7832636-7832655 | None:intergenic | 45.0% | |
| GTGGCACTATTACACATCCA+TGG | - | chr2_4:7832883-7832902 | Msa0312660:CDS | 45.0% | |
| ACCTGCAACAAGTAATGGCA+TGG | - | chr2_4:7832911-7832930 | Msa0312660:CDS | 45.0% | |
| ! | TCCATGCCATTACTTGTTGC+AGG | + | chr2_4:7832915-7832934 | None:intergenic | 45.0% |
| GTCAAACTATTGGAGTTGGG+AGG | - | chr2_4:7832938-7832957 | Msa0312660:CDS | 45.0% | |
| TTTCTCATGGGAGGAGTCTT+TGG | + | chr2_4:7832279-7832298 | None:intergenic | 45.0% | |
| !!! | TGCTGTTGTTTTCTCATGGG+AGG | + | chr2_4:7832288-7832307 | None:intergenic | 45.0% |
| TCTTGCAAGCTCTGCAGAAA+TGG | + | chr2_4:7832407-7832426 | None:intergenic | 45.0% | |
| ACTCTCAGGAATGTTCCTGT+TGG | - | chr2_4:7832447-7832466 | Msa0312660:CDS | 45.0% | |
| !! | ATGTTCCTGTTGGTGGTGTT+AGG | - | chr2_4:7832457-7832476 | Msa0312660:CDS | 45.0% |
| TTCTTCCTAACACCACCAAC+AGG | + | chr2_4:7832465-7832484 | None:intergenic | 45.0% | |
| TTCTGTGGTGGCAACAAAGA+TGG | + | chr2_4:7832636-7832655 | None:intergenic | 45.0% | |
| GTGGCACTATTACACATCCA+TGG | - | chr2_4:7832883-7832902 | Msa0312660:CDS | 45.0% | |
| ACCTGCAACAAGTAATGGCA+TGG | - | chr2_4:7832911-7832930 | Msa0312660:CDS | 45.0% | |
| ! | TCCATGCCATTACTTGTTGC+AGG | + | chr2_4:7832915-7832934 | None:intergenic | 45.0% |
| GTCAAACTATTGGAGTTGGG+AGG | - | chr2_4:7832938-7832957 | Msa0312660:CDS | 45.0% | |
| GCAGAGCTTGCAAGAGACAT+TGG | - | chr2_4:7832412-7832431 | Msa0312660:CDS | 50.0% | |
| GGACTAAAGGTGGTACTCTC+AGG | - | chr2_4:7832433-7832452 | Msa0312660:CDS | 50.0% | |
| CTCAGGAATGTTCCTGTTGG+TGG | - | chr2_4:7832450-7832469 | Msa0312660:CDS | 50.0% | |
| ! | GTTGTCATTTGAGGTGGTGG+TGG | + | chr2_4:7832559-7832578 | None:intergenic | 50.0% |
| ! | CCTGTTGTCATTTGAGGTGG+TGG | + | chr2_4:7832562-7832581 | None:intergenic | 50.0% |
| CCACCACCTCAAATGACAAC+AGG | - | chr2_4:7832559-7832578 | Msa0312660:CDS | 50.0% | |
| ! | CTGCCTGTTGTCATTTGAGG+TGG | + | chr2_4:7832565-7832584 | None:intergenic | 50.0% |
| TGTGGTGGCAACAAAGATGG+TGG | + | chr2_4:7832633-7832652 | None:intergenic | 50.0% | |
| AGTGCAATGCCAAGCACTAG+TGG | - | chr2_4:7832858-7832877 | Msa0312660:CDS | 50.0% | |
| ATGCCAAGCACTAGTGGTAG+TGG | - | chr2_4:7832864-7832883 | Msa0312660:CDS | 50.0% | |
| CTTGTTGCAGGTATCTCCCA+TGG | + | chr2_4:7832903-7832922 | None:intergenic | 50.0% | |
| GCAGAGCTTGCAAGAGACAT+TGG | - | chr2_4:7832412-7832431 | Msa0312660:CDS | 50.0% | |
| GGACTAAAGGTGGTACTCTC+AGG | - | chr2_4:7832433-7832452 | Msa0312660:CDS | 50.0% | |
| CTCAGGAATGTTCCTGTTGG+TGG | - | chr2_4:7832450-7832469 | Msa0312660:CDS | 50.0% | |
| ! | GTTGTCATTTGAGGTGGTGG+TGG | + | chr2_4:7832559-7832578 | None:intergenic | 50.0% |
| ! | CCTGTTGTCATTTGAGGTGG+TGG | + | chr2_4:7832562-7832581 | None:intergenic | 50.0% |
| CCACCACCTCAAATGACAAC+AGG | - | chr2_4:7832559-7832578 | Msa0312660:CDS | 50.0% | |
| ! | CTGCCTGTTGTCATTTGAGG+TGG | + | chr2_4:7832565-7832584 | None:intergenic | 50.0% |
| TGTGGTGGCAACAAAGATGG+TGG | + | chr2_4:7832633-7832652 | None:intergenic | 50.0% | |
| AGTGCAATGCCAAGCACTAG+TGG | - | chr2_4:7832858-7832877 | Msa0312660:CDS | 50.0% | |
| ATGCCAAGCACTAGTGGTAG+TGG | - | chr2_4:7832864-7832883 | Msa0312660:CDS | 50.0% | |
| CTTGTTGCAGGTATCTCCCA+TGG | + | chr2_4:7832903-7832922 | None:intergenic | 50.0% | |
| CAAGCTCTGCAGAAATGGCG+AGG | + | chr2_4:7832402-7832421 | None:intergenic | 55.0% | |
| !! | ATTTGAGGTGGTGGTGGAGG+AGG | + | chr2_4:7832553-7832572 | None:intergenic | 55.0% |
| !! | GTCATTTGAGGTGGTGGTGG+AGG | + | chr2_4:7832556-7832575 | None:intergenic | 55.0% |
| ! | GTGCCACTACCACTAGTGCT+TGG | + | chr2_4:7832870-7832889 | None:intergenic | 55.0% |
| CAAGCTCTGCAGAAATGGCG+AGG | + | chr2_4:7832402-7832421 | None:intergenic | 55.0% | |
| !! | ATTTGAGGTGGTGGTGGAGG+AGG | + | chr2_4:7832553-7832572 | None:intergenic | 55.0% |
| !! | GTCATTTGAGGTGGTGGTGG+AGG | + | chr2_4:7832556-7832575 | None:intergenic | 55.0% |
| ! | GTGCCACTACCACTAGTGCT+TGG | + | chr2_4:7832870-7832889 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 7832195 | 7833025 | 7832195 | ID=Msa0312660;Name=Msa0312660 |
| chr2_4 | mRNA | 7832195 | 7833025 | 7832195 | ID=Msa0312660-mRNA-1;Parent=Msa0312660;Name=Msa0312660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|276 |
| chr2_4 | exon | 7832195 | 7833025 | 7832195 | ID=Msa0312660-mRNA-1:exon:3027;Parent=Msa0312660-mRNA-1 |
| chr2_4 | CDS | 7832195 | 7833025 | 7832195 | ID=Msa0312660-mRNA-1:cds;Parent=Msa0312660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
