Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275900 | XP_013462804.1 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 1.41e-71 | 219 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0208140 | Msa0275900 | 0.808180 | 3.514396e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275900 | MtrunA17_Chr2g0288271 | 97.297 | 111 | 3 | 0 | 1 | 111 | 1 | 111 | 1.30e-75 | 219 |
| Msa0275900 | MtrunA17_Chr3g0104671 | 64.286 | 70 | 24 | 1 | 1 | 69 | 5 | 74 | 3.59e-23 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275900 | AT1G11655.1 | 47.706 | 109 | 51 | 3 | 1 | 105 | 1 | 107 | 7.01e-23 | 85.9 |
| Msa0275900 | AT4G04745.1 | 46.226 | 106 | 37 | 5 | 1 | 94 | 26 | 123 | 2.09e-18 | 75.5 |
| Msa0275900 | AT4G21902.1 | 36.538 | 104 | 50 | 4 | 1 | 94 | 1 | 98 | 1.36e-14 | 65.1 |
Find 18 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAATTGTGCTCTTCTA+TGG | 0.258118 | 2_3:-8004145 | None:intergenic |
| TCCATCTTTGAGTATGAGTA+TGG | 0.295256 | 2_3:-8004098 | None:intergenic |
| CTTCTATGGATTAATTCTTC+AGG | 0.321281 | 2_3:-8004131 | None:intergenic |
| AAAACATGGAAGCGTTTGTT+TGG | 0.344478 | 2_3:+8004341 | Msa0275900:CDS |
| GCAGCAAGAGTTGGCATTCA+TGG | 0.370072 | 2_3:+8004299 | Msa0275900:CDS |
| TGAAGATTGGAAAGAGGTTA+AGG | 0.374067 | 2_3:+8004243 | Msa0275900:CDS |
| TCCAATCTTCACCTTCAACT+TGG | 0.452781 | 2_3:-8004231 | None:intergenic |
| AATCAAAGTATCCAAGTTGA+AGG | 0.464257 | 2_3:+8004220 | Msa0275900:CDS |
| TCCAAGTTGAAGGTGAAGAT+TGG | 0.466887 | 2_3:+8004230 | Msa0275900:CDS |
| AAACATGGAAGCGTTTGTTT+GGG | 0.471262 | 2_3:+8004342 | Msa0275900:CDS |
| ACCATACTCATACTCAAAGA+TGG | 0.511994 | 2_3:+8004097 | Msa0275900:CDS |
| TGAAGGTGAAGATTGGAAAG+AGG | 0.513977 | 2_3:+8004237 | Msa0275900:CDS |
| ATTCTTGATCTACAAGGTGT+TGG | 0.525324 | 2_3:+8004160 | Msa0275900:CDS |
| AGTGTGTCTGCAGCAAGAGT+TGG | 0.566028 | 2_3:+8004290 | Msa0275900:CDS |
| AGATTGGAAAGAGGTTAAGG+AGG | 0.598888 | 2_3:+8004246 | Msa0275900:CDS |
| CAATATTTGATCATAAGAGA+AGG | 0.614835 | 2_3:-8004392 | None:intergenic |
| TTGTGAGTCAATTGAAAACA+TGG | 0.616880 | 2_3:+8004327 | Msa0275900:CDS |
| AGCACAATTCTTGATCTACA+AGG | 0.619869 | 2_3:+8004154 | Msa0275900:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAATATTTGATCATAAGAGA+AGG | - | chr2_3:8004395-8004414 | None:intergenic | 25.0% |
| ! | CTTCTATGGATTAATTCTTC+AGG | - | chr2_3:8004134-8004153 | None:intergenic | 30.0% |
| ! | AATCAAAGTATCCAAGTTGA+AGG | + | chr2_3:8004220-8004239 | Msa0275900:CDS | 30.0% |
| TTGTGAGTCAATTGAAAACA+TGG | + | chr2_3:8004327-8004346 | Msa0275900:CDS | 30.0% | |
| ACCATACTCATACTCAAAGA+TGG | + | chr2_3:8004097-8004116 | Msa0275900:CDS | 35.0% | |
| TCCATCTTTGAGTATGAGTA+TGG | - | chr2_3:8004101-8004120 | None:intergenic | 35.0% | |
| TCAAGAATTGTGCTCTTCTA+TGG | - | chr2_3:8004148-8004167 | None:intergenic | 35.0% | |
| AGCACAATTCTTGATCTACA+AGG | + | chr2_3:8004154-8004173 | Msa0275900:CDS | 35.0% | |
| ATTCTTGATCTACAAGGTGT+TGG | + | chr2_3:8004160-8004179 | Msa0275900:CDS | 35.0% | |
| TGAAGATTGGAAAGAGGTTA+AGG | + | chr2_3:8004243-8004262 | Msa0275900:CDS | 35.0% | |
| !! | AAAACATGGAAGCGTTTGTT+TGG | + | chr2_3:8004341-8004360 | Msa0275900:CDS | 35.0% |
| !! | AAACATGGAAGCGTTTGTTT+GGG | + | chr2_3:8004342-8004361 | Msa0275900:CDS | 35.0% |
| ! | TCCAAGTTGAAGGTGAAGAT+TGG | + | chr2_3:8004230-8004249 | Msa0275900:CDS | 40.0% |
| TCCAATCTTCACCTTCAACT+TGG | - | chr2_3:8004234-8004253 | None:intergenic | 40.0% | |
| TGAAGGTGAAGATTGGAAAG+AGG | + | chr2_3:8004237-8004256 | Msa0275900:CDS | 40.0% | |
| AGATTGGAAAGAGGTTAAGG+AGG | + | chr2_3:8004246-8004265 | Msa0275900:CDS | 40.0% | |
| AGTGTGTCTGCAGCAAGAGT+TGG | + | chr2_3:8004290-8004309 | Msa0275900:CDS | 50.0% | |
| GCAGCAAGAGTTGGCATTCA+TGG | + | chr2_3:8004299-8004318 | Msa0275900:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 8004080 | 8004415 | 8004080 | ID=Msa0275900;Name=Msa0275900 |
| chr2_3 | mRNA | 8004080 | 8004415 | 8004080 | ID=Msa0275900-mRNA-1;Parent=Msa0275900;Name=Msa0275900-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|111 |
| chr2_3 | exon | 8004080 | 8004415 | 8004080 | ID=Msa0275900-mRNA-1:exon:3660;Parent=Msa0275900-mRNA-1 |
| chr2_3 | CDS | 8004080 | 8004415 | 8004080 | ID=Msa0275900-mRNA-1:cds;Parent=Msa0275900-mRNA-1 |
| Gene Sequence |
| Protein sequence |
