AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0276410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276410 MtrunA17_Chr2g0288861 96.610 118 3 1 1 117 1 118 9.18e-80 229
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276410 AT3G05810.1 59.130 115 44 1 3 117 1 112 7.28e-43 136
Msa0276410 AT3G05810.2 59.130 115 44 1 3 117 1 112 7.28e-43 136

Find 64 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAGAAATTTGAGGATTT+TGG 0.099849 2_3:-8674392 None:intergenic
ATTTAACTGATTGTTTAATT+TGG 0.149784 2_3:+8677721 Msa0276410:three_prime_UTR
TCGCAATGGTTCATTTATTT+TGG 0.203119 2_3:+8677609 Msa0276410:three_prime_UTR
GCTAAGCTCGATTCCGAAAT+TGG 0.223329 2_3:+8675167 Msa0276410:CDS
ATTGCTCTCAACCCAGTTTC+TGG 0.232402 2_3:+8674443 Msa0276410:five_prime_UTR
ACAAGTACAGGCTGGGATTA+TGG 0.285458 2_3:+8677277 Msa0276410:CDS
CTTCTGGAAGTCACCAATTT+CGG 0.301377 2_3:-8675180 None:intergenic
TGGTGACTTCCAGAAGCTTC+TGG 0.312220 2_3:+8675187 Msa0276410:CDS
CTTTCGTTGTGAAATCATAT+TGG 0.332693 2_3:+8677353 Msa0276410:three_prime_UTR
ATCGGCGGTCAACCAGAAAC+TGG 0.349278 2_3:-8674455 None:intergenic
AAACATTAATGTGATGAAAA+AGG 0.355632 2_3:-8677453 None:intergenic
GTTGAAGATTGAAAGCAATA+TGG 0.363212 2_3:+8674873 Msa0276410:exon
TTCGAGTAACCAGAAGCTTC+TGG 0.370946 2_3:-8675196 None:intergenic
GCCGATACCATCTTACGTTT+CGG 0.396427 2_3:+8674472 Msa0276410:five_prime_UTR
ACCAACGTAGGGTGTTGATT+TGG 0.411994 2_3:-8674998 None:intergenic
TACCGAGCTTCTTGAGTTTG+AGG 0.413680 2_3:-8675220 None:intergenic
TGCAGTCAAGGTTGGAATAC+CGG 0.425274 2_3:+8675103 Msa0276410:intron
ACCGAGCTTCTTGAGTTTGA+GGG 0.452406 2_3:-8675219 None:intergenic
TAATTATCCTATGATTGATC+TGG 0.458874 2_3:+8677678 Msa0276410:three_prime_UTR
AAAGCAATATGGCAATGTTA+TGG 0.463415 2_3:+8674884 Msa0276410:exon
GTTTAGCCATTGTAGAAGAC+AGG 0.465918 2_3:+8677380 Msa0276410:three_prime_UTR
CAATATGATTTCACAACGAA+AGG 0.465926 2_3:-8677352 None:intergenic
ATGAATGGAGAAGAAATTTG+AGG 0.483847 2_3:-8674399 None:intergenic
CTATGATTTGCAGTCAAGGT+TGG 0.485635 2_3:+8675095 Msa0276410:intron
GAGTGTACCAGATCAATCAT+AGG 0.493903 2_3:-8677685 None:intergenic
GAGATTACCTATAAAGCAAA+GGG 0.495038 2_3:-8674499 None:intergenic
CTGATTGTTTAATTTGGAGA+AGG 0.497510 2_3:+8677727 Msa0276410:three_prime_UTR
TGAAACATGCACACAAGTAC+AGG 0.508803 2_3:+8677265 Msa0276410:CDS
ATGCAGAGAGTCATAAGAAA+CGG 0.534108 2_3:+8674913 Msa0276410:CDS
GAAACTAATCATGCTTTGAT+CGG 0.541142 2_3:-8677315 None:intergenic
TCAAGCCCTGTCTTCTACAA+TGG 0.543646 2_3:-8677386 None:intergenic
ACATGCACACAAGTACAGGC+TGG 0.552830 2_3:+8677269 Msa0276410:CDS
TTGTTTAATTTGGAGAAGGT+TGG 0.553310 2_3:+8677731 Msa0276410:three_prime_UTR
GAGAGGGAGAGATCGATGAA+TGG 0.554081 2_3:-8674414 None:intergenic
TGAGATTACCTATAAAGCAA+AGG 0.559707 2_3:-8674500 None:intergenic
ATTAACCTAGTGTACTTGAC+TGG 0.562566 2_3:-8677486 None:intergenic
AAATGACTTACATGTTTGCA+AGG 0.566149 2_3:-8675245 None:intergenic
TGAGAGCAATGGCGGTGAGA+GGG 0.568376 2_3:-8674430 None:intergenic
TAGCAAGGGGAATGAAATGC+GGG 0.569963 2_3:-8677657 None:intergenic
ACCCTCAAACTCAAGAAGCT+CGG 0.578810 2_3:+8675218 Msa0276410:CDS
GTAGGGTGTTGATTTGGAGT+AGG 0.579611 2_3:-8674992 None:intergenic
GCTTGACTCTGCATGAAAAG+AGG 0.580244 2_3:+8677403 Msa0276410:three_prime_UTR
GGGAAAACCGAAACGTAAGA+TGG 0.581590 2_3:-8674479 None:intergenic
TTGAGAGCAATGGCGGTGAG+AGG 0.583002 2_3:-8674431 None:intergenic
GCTTTGATCGGCTCAGCACG+TGG 0.589293 2_3:-8677303 None:intergenic
AATCATAGGATAATTAGCAA+GGG 0.593803 2_3:-8677671 None:intergenic
GAGGAAGTGTTACCAACGTA+GGG 0.595333 2_3:-8675009 None:intergenic
TTGCACCAGTCAAGTACACT+AGG 0.596216 2_3:+8677481 Msa0276410:three_prime_UTR
CAATCATAGGATAATTAGCA+AGG 0.596954 2_3:-8677672 None:intergenic
ACCGAAACGTAAGATGGTAT+CGG 0.607435 2_3:-8674473 None:intergenic
AGAAACTGGGTTGAGAGCAA+TGG 0.609889 2_3:-8674441 None:intergenic
TTTAGCCATTGTAGAAGACA+GGG 0.612180 2_3:+8677381 Msa0276410:three_prime_UTR
CATGCACACAAGTACAGGCT+GGG 0.612543 2_3:+8677270 Msa0276410:CDS
TCGGCGGTCAACCAGAAACT+GGG 0.612713 2_3:-8674454 None:intergenic
AACTGGGTTGAGAGCAATGG+CGG 0.614698 2_3:-8674438 None:intergenic
TCATGAAAGAGATAGAGCAC+TGG 0.619032 2_3:-8674357 None:intergenic
AGACGAAGAATGTGAGTGTG+TGG 0.622529 2_3:-8674953 None:intergenic
GGAGGAAGTGTTACCAACGT+AGG 0.635206 2_3:-8675010 None:intergenic
ATCATAGGATAATTAGCAAG+GGG 0.635438 2_3:-8677670 None:intergenic
TCCAAATCAACACCCTACGT+TGG 0.650781 2_3:+8674997 Msa0276410:CDS
TTAGCAAGGGGAATGAAATG+CGG 0.660547 2_3:-8677658 None:intergenic
TTGGGATTAAGAGTTCGCAA+TGG 0.663627 2_3:+8677595 Msa0276410:three_prime_UTR
AGTACAGGCTGGGATTATGG+AGG 0.691324 2_3:+8677280 Msa0276410:CDS
GAAACGTAAGATGGTATCGG+CGG 0.741573 2_3:-8674470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTGTTTTATGATAAAA+TGG + chr2_3:8674650-8674669 Msa0276410:intron 10.0%
!!! TTATTGTTTTATGATAAAAT+GGG + chr2_3:8674651-8674670 Msa0276410:intron 10.0%
!!! TTGTAGAATTAATTGTTTTT+TGG + chr2_3:8674626-8674645 Msa0276410:intron 15.0%
!!! TGTAGAATTAATTGTTTTTT+GGG + chr2_3:8674627-8674646 Msa0276410:intron 15.0%
!!! TATTGTTTTATGATAAAATG+GGG + chr2_3:8674652-8674671 Msa0276410:intron 15.0%
!! TTCTTATTAGAGAATAATAT+TGG + chr2_3:8675403-8675422 Msa0276410:intron 15.0%
!! AAATACAATTTAATTGTAGA+AGG - chr2_3:8675765-8675784 None:intergenic 15.0%
!! TCTACAATTAAATTGTATTT+CGG + chr2_3:8675765-8675784 Msa0276410:intron 15.0%
!!! CTTTTATAATTTTTCCTATT+AGG - chr2_3:8675940-8675959 None:intergenic 15.0%
!! CTAATAGGAAAAATTATAAA+AGG + chr2_3:8675938-8675957 Msa0276410:intron 15.0%
!!! ACAGTTTTTTTACAAAAAAA+TGG - chr2_3:8676135-8676154 None:intergenic 15.0%
!!! ATTTTTGTATATTTTATGAC+TGG + chr2_3:8676301-8676320 Msa0276410:intron 15.0%
!! GTTAAATTTATCGAAATTTA+AGG + chr2_3:8676361-8676380 Msa0276410:intron 15.0%
!!! TTTTTTTAAATACCATACTT+AGG + chr2_3:8676892-8676911 Msa0276410:intron 15.0%
!! AATATAAAATGAAACTTGTA+TGG + chr2_3:8677024-8677043 Msa0276410:intron 15.0%
!! ATTTAACTGATTGTTTAATT+TGG + chr2_3:8677721-8677740 Msa0276410:three_prime_UTR 15.0%
!!! ATTTTTAAATGGAATTGGAA+AGG + chr2_3:8675127-8675146 Msa0276410:CDS 20.0%
!!! TTTTTAAATGGAATTGGAAA+GGG + chr2_3:8675128-8675147 Msa0276410:CDS 20.0%
!! CAATTAATTTCTACCAATAA+GGG - chr2_3:8675377-8675396 None:intergenic 20.0%
!! ACAATTAATTTCTACCAATA+AGG - chr2_3:8675378-8675397 None:intergenic 20.0%
!!! CAGTTATGTTGTTTATTTTT+AGG + chr2_3:8675496-8675515 Msa0276410:intron 20.0%
!! TAGGTAATTGAAGATATTAA+AGG - chr2_3:8675921-8675940 None:intergenic 20.0%
!! AATATCTTCAATTACCTAAT+AGG + chr2_3:8675923-8675942 Msa0276410:intron 20.0%
!! ATATAGAGTTAAAAGACAAA+TGG - chr2_3:8675996-8676015 None:intergenic 20.0%
!!! GTTTTTTTACAAAAAAATGG+TGG - chr2_3:8676132-8676151 None:intergenic 20.0%
!! TTGTAACTTTAAATTGAAAC+TGG + chr2_3:8676269-8676288 Msa0276410:intron 20.0%
!! ACTTATTTATTTGTAAGACA+AGG - chr2_3:8676453-8676472 None:intergenic 20.0%
!! ACAAATAAATAAGTTGTCAT+AGG + chr2_3:8676459-8676478 Msa0276410:intron 20.0%
!!! GTAACAATTTTAGCTTTTAA+TGG + chr2_3:8676510-8676529 Msa0276410:intron 20.0%
!! ATGTAAGATACAAAATAATC+TGG - chr2_3:8676931-8676950 None:intergenic 20.0%
!! AAACATTAATGTGATGAAAA+AGG - chr2_3:8677456-8677475 None:intergenic 20.0%
!! AGAAGAAATTTGAGGATTTT+GGG - chr2_3:8674394-8674413 None:intergenic 25.0%
!!! TTCTTTTTAGTCATTTTGTG+CGG + chr2_3:8674550-8674569 Msa0276410:intron 25.0%
!!! ATTTTGTTCTTCTTGTTTCT+GGG + chr2_3:8674600-8674619 Msa0276410:intron 25.0%
! AAAATAAGCTGAAAAAACAC+TGG - chr2_3:8674738-8674757 None:intergenic 25.0%
!!! ATGAAAAGGTGTTAAGTAAA+GGG - chr2_3:8675042-8675061 None:intergenic 25.0%
!!! AATGAAAAGGTGTTAAGTAA+AGG - chr2_3:8675043-8675062 None:intergenic 25.0%
! ACAATTGATGAGTGTTAAAA+AGG - chr2_3:8675067-8675086 None:intergenic 25.0%
!! TTTTAACACTCATCAATTGT+TGG + chr2_3:8675067-8675086 Msa0276410:intron 25.0%
! CCAATTCCATTTAAAAATTC+CGG - chr2_3:8675125-8675144 None:intergenic 25.0%
!!! TTTTAGGAATCAATAGAGAA+AGG + chr2_3:8675512-8675531 Msa0276410:intron 25.0%
! AAGGAGAAAGTGATTATTTA+TGG + chr2_3:8675536-8675555 Msa0276410:intron 25.0%
! AGGAGAAAGTGATTATTTAT+GGG + chr2_3:8675537-8675556 Msa0276410:intron 25.0%
! ACATAAATTAAGGAAAGAGA+TGG - chr2_3:8675668-8675687 None:intergenic 25.0%
!!! TCAACTAGATCAGTTATTTT+GGG - chr2_3:8675867-8675886 None:intergenic 25.0%
!! ATCAACTAGATCAGTTATTT+TGG - chr2_3:8675868-8675887 None:intergenic 25.0%
!! TGAAATAAGATCAGTCATTT+TGG + chr2_3:8676072-8676091 Msa0276410:intron 25.0%
!!! GAAATAAGATCAGTCATTTT+GGG + chr2_3:8676073-8676092 Msa0276410:intron 25.0%
!! ACTGTTATTAGTTTAAGTCA+CGG + chr2_3:8676150-8676169 Msa0276410:intron 25.0%
! TTGTATGTAATTCTTATGAC+TGG + chr2_3:8676244-8676263 Msa0276410:intron 25.0%
!! ATTTATCGAAATTTAAGGCT+TGG + chr2_3:8676366-8676385 Msa0276410:intron 25.0%
!! TTTTAAATACCATACTTAGG+AGG + chr2_3:8676895-8676914 Msa0276410:intron 25.0%
!!! GACTTTATTTTGATTGTGAT+TGG + chr2_3:8677165-8677184 Msa0276410:intron 25.0%
! TTAATGTGATGAAAAAGGAT+GGG - chr2_3:8677451-8677470 None:intergenic 25.0%
! ATTAATGTGATGAAAAAGGA+TGG - chr2_3:8677452-8677471 None:intergenic 25.0%
! GCAAAAAGAGAATTAACTTT+GGG + chr2_3:8677577-8677596 Msa0276410:three_prime_UTR 25.0%
! AATCATAGGATAATTAGCAA+GGG - chr2_3:8677674-8677693 None:intergenic 25.0%
! TAATTATCCTATGATTGATC+TGG + chr2_3:8677678-8677697 Msa0276410:three_prime_UTR 25.0%
! GAAGAAATTTGAGGATTTTG+GGG - chr2_3:8674393-8674412 None:intergenic 30.0%
! GAGAAGAAATTTGAGGATTT+TGG - chr2_3:8674395-8674414 None:intergenic 30.0%
ATGAATGGAGAAGAAATTTG+AGG - chr2_3:8674402-8674421 None:intergenic 30.0%
GAGATTACCTATAAAGCAAA+GGG - chr2_3:8674502-8674521 None:intergenic 30.0%
TGAGATTACCTATAAAGCAA+AGG - chr2_3:8674503-8674522 None:intergenic 30.0%
!! GATTTTGTTCTTCTTGTTTC+TGG + chr2_3:8674599-8674618 Msa0276410:intron 30.0%
!!! ATGGGGTCATTTTTATTTGA+TGG + chr2_3:8674669-8674688 Msa0276410:intron 30.0%
! TTGATGGAATTGTGTCTTTT+TGG + chr2_3:8674685-8674704 Msa0276410:intron 30.0%
GATGAAGATTGAAAGCAATA+TGG + chr2_3:8674783-8674802 Msa0276410:intron 30.0%
TAAGGACATAAGTCAAATCA+GGG - chr2_3:8674842-8674861 None:intergenic 30.0%
TTATGTCCTTACGTGATATT+AGG + chr2_3:8674851-8674870 Msa0276410:intron 30.0%
GTTGAAGATTGAAAGCAATA+TGG + chr2_3:8674873-8674892 Msa0276410:exon 30.0%
AAAGCAATATGGCAATGTTA+TGG + chr2_3:8674794-8674813 Msa0276410:intron 30.0%
GTGTTAAAAAGGCAATGAAA+AGG - chr2_3:8675056-8675075 None:intergenic 30.0%
! GGAATACCGGAATTTTTAAA+TGG + chr2_3:8675116-8675135 Msa0276410:CDS 30.0%
! CCGGAATTTTTAAATGGAAT+TGG + chr2_3:8675122-8675141 Msa0276410:CDS 30.0%
AAATGACTTACATGTTTGCA+AGG - chr2_3:8675248-8675267 None:intergenic 30.0%
AATTATTCATACGAATGCCA+TGG - chr2_3:8675275-8675294 None:intergenic 30.0%
TTACAAGTTCATGTGCTTAT+TGG + chr2_3:8675329-8675348 Msa0276410:intron 30.0%
AGTTATGAACATCTAGTCAT+GGG + chr2_3:8675453-8675472 Msa0276410:intron 30.0%
TATTTATGGGAGTTGTTGAA+TGG + chr2_3:8675550-8675569 Msa0276410:intron 30.0%
AAATTCTCTACTTCTTGGAA+GGG + chr2_3:8675591-8675610 Msa0276410:intron 30.0%
CAGAATGCACACATAAATTA+AGG - chr2_3:8675678-8675697 None:intergenic 30.0%
TAGTTAACAATCCTATCCAA+CGG - chr2_3:8675831-8675850 None:intergenic 30.0%
! ATGACTGATCTTATTTCACT+AGG - chr2_3:8676070-8676089 None:intergenic 30.0%
! TTACAAAAAAATGGTGGAAG+AGG - chr2_3:8676126-8676145 None:intergenic 30.0%
AGTTTAAGTCACGGTTAATT+TGG + chr2_3:8676159-8676178 Msa0276410:intron 30.0%
!! TTAGCTTTAAAATGACCAGA+TGG + chr2_3:8676327-8676346 Msa0276410:intron 30.0%
TAACAAAAATCAGATCCATC+TGG - chr2_3:8676345-8676364 None:intergenic 30.0%
! AAATTTAAGGCTTGGATGTT+AGG + chr2_3:8676374-8676393 Msa0276410:intron 30.0%
TTAGGACTTAGGATTATCAA+AGG + chr2_3:8676392-8676411 Msa0276410:intron 30.0%
! TACAATGACCTATCAACTTT+TGG + chr2_3:8677102-8677121 Msa0276410:intron 30.0%
! TATCAACTTTTGGTAGGATT+GGG + chr2_3:8677112-8677131 Msa0276410:intron 30.0%
GAAACTAATCATGCTTTGAT+CGG - chr2_3:8677318-8677337 None:intergenic 30.0%
CAATATGATTTCACAACGAA+AGG - chr2_3:8677355-8677374 None:intergenic 30.0%
CTTTCGTTGTGAAATCATAT+TGG + chr2_3:8677353-8677372 Msa0276410:three_prime_UTR 30.0%
! TTTTTGTCTTGTATCACTCT+TGG + chr2_3:8677555-8677574 Msa0276410:three_prime_UTR 30.0%
GGCAAAAAGAGAATTAACTT+TGG + chr2_3:8677576-8677595 Msa0276410:three_prime_UTR 30.0%
! TCGCAATGGTTCATTTATTT+TGG + chr2_3:8677609-8677628 Msa0276410:three_prime_UTR 30.0%
ATCATAGGATAATTAGCAAG+GGG - chr2_3:8677673-8677692 None:intergenic 30.0%
CAATCATAGGATAATTAGCA+AGG - chr2_3:8677675-8677694 None:intergenic 30.0%
!! CTGATTGTTTAATTTGGAGA+AGG + chr2_3:8677727-8677746 Msa0276410:three_prime_UTR 30.0%
!! TTGTTTAATTTGGAGAAGGT+TGG + chr2_3:8677731-8677750 Msa0276410:three_prime_UTR 30.0%
!! TTTGAGGATTTTGGGGATTT+AGG - chr2_3:8674386-8674405 None:intergenic 35.0%
CTTTCAATCTTCATCAACTC+GGG - chr2_3:8674778-8674797 None:intergenic 35.0%
GCTTTCAATCTTCATCAACT+CGG - chr2_3:8674779-8674798 None:intergenic 35.0%
GTAAGGACATAAGTCAAATC+AGG - chr2_3:8674843-8674862 None:intergenic 35.0%
CTTCAACCTAATATCACGTA+AGG - chr2_3:8674860-8674879 None:intergenic 35.0%
ATGCAGAGAGTCATAAGAAA+CGG + chr2_3:8674913-8674932 Msa0276410:CDS 35.0%
TTAAGTAAAGGGAAAAGAGG+AGG - chr2_3:8675031-8675050 None:intergenic 35.0%
GTGTTAAGTAAAGGGAAAAG+AGG - chr2_3:8675034-8675053 None:intergenic 35.0%
! TATGCTATGATTTGCAGTCA+AGG + chr2_3:8675091-8675110 Msa0276410:intron 35.0%
!! ATGTAAGTCATTTTCCTCCA+TGG + chr2_3:8675255-8675274 Msa0276410:intron 35.0%
AATAAGGGTTGCACAAGATA+GGG - chr2_3:8675362-8675381 None:intergenic 35.0%
CAGTTATGAACATCTAGTCA+TGG + chr2_3:8675452-8675471 Msa0276410:intron 35.0%
GTTATGAACATCTAGTCATG+GGG + chr2_3:8675454-8675473 Msa0276410:intron 35.0%
GGAATCAATAGAGAAAGGAA+AGG + chr2_3:8675517-8675536 Msa0276410:intron 35.0%
AGACCAAATTCTCTACTTCT+TGG + chr2_3:8675586-8675605 Msa0276410:intron 35.0%
CTTCCAAGAAGTAGAGAATT+TGG - chr2_3:8675592-8675611 None:intergenic 35.0%
CAAATTCTCTACTTCTTGGA+AGG + chr2_3:8675590-8675609 Msa0276410:intron 35.0%
! AAGAGATGGTCTGTTTTACA+AGG - chr2_3:8675654-8675673 None:intergenic 35.0%
!!! CTAGATCAGTTATTTTGGGA+CGG - chr2_3:8675863-8675882 None:intergenic 35.0%
TAAAGGTCAAAATTGTGCAC+TGG - chr2_3:8675904-8675923 None:intergenic 35.0%
!! TAAGATCAGTCATTTTGGGA+CGG + chr2_3:8676077-8676096 Msa0276410:intron 35.0%
TGAATTATTAAGGGGGGAAA+GGG - chr2_3:8676189-8676208 None:intergenic 35.0%
TAAGACAAGGAAGCAACATA+CGG - chr2_3:8676440-8676459 None:intergenic 35.0%
! GCAAGTTAGTACTAGTCTAA+TGG - chr2_3:8676679-8676698 None:intergenic 35.0%
!! GCATGTTACACATGTTTTTG+TGG + chr2_3:8676706-8676725 Msa0276410:intron 35.0%
!!! GATGTGTTGTCATTTTCATC+TGG + chr2_3:8676741-8676760 Msa0276410:intron 35.0%
! ATCTAAGATCCTCCTAAGTA+TGG - chr2_3:8676907-8676926 None:intergenic 35.0%
TGCATCATTAGCTAATTCCA+AGG + chr2_3:8676977-8676996 Msa0276410:intron 35.0%
!! TCTGTTGTTGACTCTGATAT+TGG + chr2_3:8677000-8677019 Msa0276410:intron 35.0%
AGTCACTTGTGATGCAATTA+TGG + chr2_3:8677048-8677067 Msa0276410:intron 35.0%
! ATGACCTATCAACTTTTGGT+AGG + chr2_3:8677106-8677125 Msa0276410:intron 35.0%
! CAATCCTACCAAAAGTTGAT+AGG - chr2_3:8677113-8677132 None:intergenic 35.0%
! CTATCAACTTTTGGTAGGAT+TGG + chr2_3:8677111-8677130 Msa0276410:intron 35.0%
! ATCAACTTTTGGTAGGATTG+GGG + chr2_3:8677113-8677132 Msa0276410:intron 35.0%
CTAGCTAATTGTATGTCATG+TGG - chr2_3:8677191-8677210 None:intergenic 35.0%
! AACTAGTTTATCCTTTGCAG+AGG + chr2_3:8677232-8677251 Msa0276410:intron 35.0%
! AATATTAGCTTCCTCTGCAA+AGG - chr2_3:8677246-8677265 None:intergenic 35.0%
TTTAGCCATTGTAGAAGACA+GGG + chr2_3:8677381-8677400 Msa0276410:three_prime_UTR 35.0%
! GCATGAAAAGAGGTAGTTTT+TGG + chr2_3:8677413-8677432 Msa0276410:three_prime_UTR 35.0%
ATTAACCTAGTGTACTTGAC+TGG - chr2_3:8677489-8677508 None:intergenic 35.0%
TCATGAAAGAGATAGAGCAC+TGG - chr2_3:8674360-8674379 None:intergenic 40.0%
ACCGAAACGTAAGATGGTAT+CGG - chr2_3:8674476-8674495 None:intergenic 40.0%
! CGGTTTTCCCTTTGCTTTAT+AGG + chr2_3:8674492-8674511 Msa0276410:five_prime_UTR 40.0%
! CTATGATTTGCAGTCAAGGT+TGG + chr2_3:8675095-8675114 Msa0276410:intron 40.0%
! CTTCTGGAAGTCACCAATTT+CGG - chr2_3:8675183-8675202 None:intergenic 40.0%
TATTCATACGAATGCCATGG+AGG - chr2_3:8675272-8675291 None:intergenic 40.0%
CAATAAGGGTTGCACAAGAT+AGG - chr2_3:8675363-8675382 None:intergenic 40.0%
CTATCTTGTGCAACCCTTAT+TGG + chr2_3:8675361-8675380 Msa0276410:intron 40.0%
TGGGAGTTGTTGAATGGTTA+TGG + chr2_3:8675556-8675575 Msa0276410:intron 40.0%
GGGAGTTGTTGAATGGTTAT+GGG + chr2_3:8675557-8675576 Msa0276410:intron 40.0%
AGTTGTTGAATGGTTATGGG+AGG + chr2_3:8675560-8675579 Msa0276410:intron 40.0%
GTTGTTGAATGGTTATGGGA+GGG + chr2_3:8675561-8675580 Msa0276410:intron 40.0%
ACCAAGACTCTCGTATTTCT+TGG + chr2_3:8675618-8675637 Msa0276410:intron 40.0%
TCCAAGAAATACGAGAGTCT+TGG - chr2_3:8675622-8675641 None:intergenic 40.0%
GACTCTCGTATTTCTTGGAT+AGG + chr2_3:8675623-8675642 Msa0276410:intron 40.0%
ACTCGCATTCTGTTAGAAAC+TGG - chr2_3:8675793-8675812 None:intergenic 40.0%
!!! ATCAGTTATTTTGGGACGGA+AGG - chr2_3:8675859-8675878 None:intergenic 40.0%
CTGAATTATTAAGGGGGGAA+AGG - chr2_3:8676190-8676209 None:intergenic 40.0%
AGCCTGCTGAATTATTAAGG+GGG - chr2_3:8676196-8676215 None:intergenic 40.0%
CAGCCTGCTGAATTATTAAG+GGG - chr2_3:8676197-8676216 None:intergenic 40.0%
CCAGCCTGCTGAATTATTAA+GGG - chr2_3:8676198-8676217 None:intergenic 40.0%
CCTTAATAATTCAGCAGGCT+GGG + chr2_3:8676196-8676215 Msa0276410:intron 40.0%
AATCAATTGCTGTAGGCAGA+GGG - chr2_3:8676625-8676644 None:intergenic 40.0%
AAATCAATTGCTGTAGGCAG+AGG - chr2_3:8676626-8676645 None:intergenic 40.0%
GTGCAGAAATCAATTGCTGT+AGG - chr2_3:8676632-8676651 None:intergenic 40.0%
!!! GTTGTCATTTTCATCTGGCA+AGG + chr2_3:8676746-8676765 Msa0276410:intron 40.0%
GGCAAATGCCTATGTTTGAT+AGG + chr2_3:8676767-8676786 Msa0276410:intron 40.0%
AATGCCTATGTTTGATAGGC+AGG + chr2_3:8676771-8676790 Msa0276410:intron 40.0%
TACACCTGCCTATCAAACAT+AGG - chr2_3:8676778-8676797 None:intergenic 40.0%
TGATCCCGATCAGTTACAAT+TGG + chr2_3:8676838-8676857 Msa0276410:intron 40.0%
GATCCCGATCAGTTACAATT+GGG + chr2_3:8676839-8676858 Msa0276410:intron 40.0%
TGACCCAATTGTAACTGATC+GGG - chr2_3:8676845-8676864 None:intergenic 40.0%
CTGACCCAATTGTAACTGAT+CGG - chr2_3:8676846-8676865 None:intergenic 40.0%
TGTATCTTACATCTGTGCTC+TGG + chr2_3:8676939-8676958 Msa0276410:intron 40.0%
CATACAATTAGCTAGTCCAC+CGG + chr2_3:8677196-8677215 Msa0276410:intron 40.0%
CTAGTTGTGAGAAAGTTTGC+CGG - chr2_3:8677218-8677237 None:intergenic 40.0%
TGAAACATGCACACAAGTAC+AGG + chr2_3:8677265-8677284 Msa0276410:CDS 40.0%
GTTTAGCCATTGTAGAAGAC+AGG + chr2_3:8677380-8677399 Msa0276410:three_prime_UTR 40.0%
!!! AAAGAGGTAGTTTTTGGCTC+TGG + chr2_3:8677419-8677438 Msa0276410:three_prime_UTR 40.0%
ATGTGATGAAAAAGGATGGG+AGG - chr2_3:8677448-8677467 None:intergenic 40.0%
TTGGGATTAAGAGTTCGCAA+TGG + chr2_3:8677595-8677614 Msa0276410:three_prime_UTR 40.0%
TTAGCAAGGGGAATGAAATG+CGG - chr2_3:8677661-8677680 None:intergenic 40.0%
GAGTGTACCAGATCAATCAT+AGG - chr2_3:8677688-8677707 None:intergenic 40.0%
! AGAAACTGGGTTGAGAGCAA+TGG - chr2_3:8674444-8674463 None:intergenic 45.0%
ATTGCTCTCAACCCAGTTTC+TGG + chr2_3:8674443-8674462 Msa0276410:five_prime_UTR 45.0%
! GAAACGTAAGATGGTATCGG+CGG - chr2_3:8674473-8674492 None:intergenic 45.0%
GCCGATACCATCTTACGTTT+CGG + chr2_3:8674472-8674491 Msa0276410:five_prime_UTR 45.0%
GGGAAAACCGAAACGTAAGA+TGG - chr2_3:8674482-8674501 None:intergenic 45.0%
AGACGAAGAATGTGAGTGTG+TGG - chr2_3:8674956-8674975 None:intergenic 45.0%
!! GTAGGGTGTTGATTTGGAGT+AGG - chr2_3:8674995-8675014 None:intergenic 45.0%
TCCAAATCAACACCCTACGT+TGG + chr2_3:8674997-8675016 Msa0276410:CDS 45.0%
!! ACCAACGTAGGGTGTTGATT+TGG - chr2_3:8675001-8675020 None:intergenic 45.0%
!! GAGGAAGTGTTACCAACGTA+GGG - chr2_3:8675012-8675031 None:intergenic 45.0%
TGCAGTCAAGGTTGGAATAC+CGG + chr2_3:8675103-8675122 Msa0276410:intron 45.0%
GCTAAGCTCGATTCCGAAAT+TGG + chr2_3:8675167-8675186 Msa0276410:CDS 45.0%
TTCGAGTAACCAGAAGCTTC+TGG - chr2_3:8675199-8675218 None:intergenic 45.0%
ACCCTCAAACTCAAGAAGCT+CGG + chr2_3:8675218-8675237 Msa0276410:CDS 45.0%
ACCGAGCTTCTTGAGTTTGA+GGG - chr2_3:8675222-8675241 None:intergenic 45.0%
TACCGAGCTTCTTGAGTTTG+AGG - chr2_3:8675223-8675242 None:intergenic 45.0%
TGCATGTGTGATAAAGCCGT+TGG + chr2_3:8675812-8675831 Msa0276410:intron 45.0%
GTGTGATAAAGCCGTTGGAT+AGG + chr2_3:8675817-8675836 Msa0276410:intron 45.0%
!!! ATCAGTCATTTTGGGACGGA+GGG + chr2_3:8676081-8676100 Msa0276410:intron 45.0%
TCCCCCCTTAATAATTCAGC+AGG + chr2_3:8676191-8676210 Msa0276410:intron 45.0%
GCCTGCTGAATTATTAAGGG+GGG - chr2_3:8676195-8676214 None:intergenic 45.0%
CCCTTAATAATTCAGCAGGC+TGG + chr2_3:8676195-8676214 Msa0276410:intron 45.0%
CCCAGCCTGCTGAATTATTA+AGG - chr2_3:8676199-8676218 None:intergenic 45.0%
AGGCTTGGATGTTAGGACTT+AGG + chr2_3:8676381-8676400 Msa0276410:intron 45.0%
AAGGCATGCAGAGAGTCTTT+AGG + chr2_3:8676411-8676430 Msa0276410:intron 45.0%
! ACCTGCAGCTTTTACTGTTC+AGG + chr2_3:8676584-8676603 Msa0276410:intron 45.0%
ACCTGAACAGTAAAAGCTGC+AGG - chr2_3:8676588-8676607 None:intergenic 45.0%
TCAGAGTCAACAACAGACCT+TGG - chr2_3:8676997-8677016 None:intergenic 45.0%
ACAAGTACAGGCTGGGATTA+TGG + chr2_3:8677277-8677296 Msa0276410:CDS 45.0%
TCAAGCCCTGTCTTCTACAA+TGG - chr2_3:8677389-8677408 None:intergenic 45.0%
GCTTGACTCTGCATGAAAAG+AGG + chr2_3:8677403-8677422 Msa0276410:three_prime_UTR 45.0%
TTGCACCAGTCAAGTACACT+AGG + chr2_3:8677481-8677500 Msa0276410:three_prime_UTR 45.0%
TAGCAAGGGGAATGAAATGC+GGG - chr2_3:8677660-8677679 None:intergenic 45.0%
!! ATTTAAAAAAAATGTATTTA+AGG - chr2_3:8676883-8676902 None:intergenic 5.0%
GAGAGGGAGAGATCGATGAA+TGG - chr2_3:8674417-8674436 None:intergenic 50.0%
! AACTGGGTTGAGAGCAATGG+CGG - chr2_3:8674441-8674460 None:intergenic 50.0%
!! GGAGGAAGTGTTACCAACGT+AGG - chr2_3:8675013-8675032 None:intergenic 50.0%
TGGTGACTTCCAGAAGCTTC+TGG + chr2_3:8675187-8675206 Msa0276410:CDS 50.0%
!!! GATCAGTCATTTTGGGACGG+AGG + chr2_3:8676080-8676099 Msa0276410:intron 50.0%
! CTAGCGTCTAAGATGCCTGT+AGG + chr2_3:8676811-8676830 Msa0276410:intron 50.0%
GATCGGGATCATATGCCTAC+AGG - chr2_3:8676829-8676848 None:intergenic 50.0%
GTTGTGAGAAAGTTTGCCGG+TGG - chr2_3:8677215-8677234 None:intergenic 50.0%
ACATGCACACAAGTACAGGC+TGG + chr2_3:8677269-8677288 Msa0276410:CDS 50.0%
CATGCACACAAGTACAGGCT+GGG + chr2_3:8677270-8677289 Msa0276410:CDS 50.0%
AGTACAGGCTGGGATTATGG+AGG + chr2_3:8677280-8677299 Msa0276410:CDS 50.0%
TGAGAGCAATGGCGGTGAGA+GGG - chr2_3:8674433-8674452 None:intergenic 55.0%
TTGAGAGCAATGGCGGTGAG+AGG - chr2_3:8674434-8674453 None:intergenic 55.0%
TCGGCGGTCAACCAGAAACT+GGG - chr2_3:8674457-8674476 None:intergenic 55.0%
ATCGGCGGTCAACCAGAAAC+TGG - chr2_3:8674458-8674477 None:intergenic 55.0%
! GCTTTGATCGGCTCAGCACG+TGG - chr2_3:8677306-8677325 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 8674350 8677763 8674350 ID=Msa0276410;Name=Msa0276410
chr2_3 mRNA 8674350 8677763 8674350 ID=Msa0276410-mRNA-1;Parent=Msa0276410;Name=Msa0276410-mRNA-1;_AED=0.07;_eAED=0.07;_QI=183|1|1|1|0.66|0.5|4|434|117
chr2_3 exon 8674350 8674513 8674350 ID=Msa0276410-mRNA-1:exon:3884;Parent=Msa0276410-mRNA-1
chr2_3 exon 8674873 8675018 8674873 ID=Msa0276410-mRNA-1:exon:3885;Parent=Msa0276410-mRNA-1
chr2_3 exon 8675108 8675256 8675108 ID=Msa0276410-mRNA-1:exon:3886;Parent=Msa0276410-mRNA-1
chr2_3 exon 8677252 8677763 8677252 ID=Msa0276410-mRNA-1:exon:3887;Parent=Msa0276410-mRNA-1
chr2_3 five_prime_UTR 8674350 8674513 8674350 ID=Msa0276410-mRNA-1:five_prime_utr;Parent=Msa0276410-mRNA-1
chr2_3 five_prime_UTR 8674873 8674891 8674873 ID=Msa0276410-mRNA-1:five_prime_utr;Parent=Msa0276410-mRNA-1
chr2_3 CDS 8674892 8675018 8674892 ID=Msa0276410-mRNA-1:cds;Parent=Msa0276410-mRNA-1
chr2_3 CDS 8675108 8675256 8675108 ID=Msa0276410-mRNA-1:cds;Parent=Msa0276410-mRNA-1
chr2_3 CDS 8677252 8677329 8677252 ID=Msa0276410-mRNA-1:cds;Parent=Msa0276410-mRNA-1
chr2_3 three_prime_UTR 8677330 8677763 8677330 ID=Msa0276410-mRNA-1:three_prime_utr;Parent=Msa0276410-mRNA-1
Gene Sequence

>Msa0276410

ATGGCAATGTTATGGTTGCAGATGCAGAGAGTCATAAGAAACGGAAGTGTTAATTTGATTTCCACACACTCACATTCTTCGTCTTACTTTTCTTCGAGATCCTACTCCAAATCAACACCCTACGTTGTCAAGGTTGGAATACCGGAATTTTTAAATGGAATTGGAAAGGGAGTCGAATCTCATGTCGCTAAGCTCGATTCCGAAATTGGTGACTTCCAGAAGCTTCTGGTTACTCGAACCCTCAAACTCAAGAAGCTCGGTATTCCTTGCAAACATAGGAAGCTAATATTGAAACATGCACACAAGTACAGGCTGGGATTATGGAGGCCACGTGCTGAGCCGATCAAAGCATGA
Protein sequence

>Msa0276410

MAMLWLQMQRVIRNGSVNLISTHSHSSSYFSSRSYSKSTPYVVKVGIPEFLNGIGKGVESHVAKLDSEIGDFQKLLVTRTLKLKKLGIPCKHRKLILKHAHKYRLGLWRPRAEPIKA*