AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0280860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280860 MtrunA17_Chr2g0294911 93.258 178 11 1 1 178 430 606 2.06e-118 347
Msa0280860 MtrunA17_Chr4g0067981 56.150 187 65 5 3 173 495 680 2.70e-58 193
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280860 AT3G16857.2 37.363 182 94 9 1 173 520 690 1.54e-18 82.8
Msa0280860 AT3G16857.3 37.363 182 94 9 1 173 450 620 1.61e-18 82.4

Find 46 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTGGAATTAAAGGATTA+GGG 0.249480 2_3:-15910133 Msa0280860:CDS
GAAGCTACTTCTGGAATTAA+AGG 0.261827 2_3:-15910140 Msa0280860:CDS
ACTTCTGGAATTAAAGGATT+AGG 0.286054 2_3:-15910134 Msa0280860:CDS
TTTCATGCGGAAGCTACTTC+TGG 0.314408 2_3:-15910149 Msa0280860:CDS
GATAATGAGTTTGACTTTGA+TGG 0.349646 2_3:-15909627 Msa0280860:CDS
TGAGGTGTTGACCAACATTA+TGG 0.359965 2_3:+15909904 None:intergenic
TAACAGGATCTGTTATAAAC+TGG 0.403457 2_3:+15909585 None:intergenic
ATTTGCTAATCCCCAATCAT+TGG 0.406561 2_3:+15910070 None:intergenic
CCATCAGAAGTCCAATGATT+GGG 0.416956 2_3:-15910081 Msa0280860:CDS
TCCATCAGAAGTCCAATGAT+TGG 0.463174 2_3:-15910082 Msa0280860:CDS
TGATTAGAAAGAGAGCTAAC+AGG 0.464856 2_3:+15909569 None:intergenic
TTCTCGCAGCACAAAGGCGT+TGG 0.468445 2_3:-15909657 Msa0280860:intron
CCCAATCATTGGACTTCTGA+TGG 0.470889 2_3:+15910081 None:intergenic
TTGTCGAGTCTGTTGGATAG+AGG 0.472904 2_3:+15909956 None:intergenic
CTTAATGAATTGTCGACAAA+AGG 0.475530 2_3:+15909876 None:intergenic
AGATCAATGTTGCCTTGTAA+AGG 0.489412 2_3:+15910011 None:intergenic
TTCCCTCTTAGATGCACTCC+GGG 0.489466 2_3:-15910200 Msa0280860:CDS
CAACATGCAAATCCTTTACA+AGG 0.495519 2_3:-15910023 Msa0280860:CDS
GTTGAGAAGCATCATATGTC+AGG 0.515544 2_3:+15910042 None:intergenic
CATGAGCGTATTTCTGAACC+AGG 0.515894 2_3:-15909778 Msa0280860:intron
GATTGGGGATTAGCAAATAC+AGG 0.520281 2_3:-15910065 Msa0280860:CDS
ATATCCAGTAGCAGTACAAA+AGG 0.527621 2_3:-15910176 Msa0280860:CDS
TGACTGACTTGATCAGAGAA+GGG 0.534175 2_3:+15909810 None:intergenic
TTGACTGACTTGATCAGAGA+AGG 0.537467 2_3:+15909809 None:intergenic
ATACCCGGAGTGCATCTAAG+AGG 0.561462 2_3:+15910197 None:intergenic
TAGTTGCCAATGTAAATAGT+TGG 0.567960 2_3:-15909546 Msa0280860:three_prime_UTR
GGAACACCAACTATTTACAT+TGG 0.570285 2_3:+15909540 None:intergenic
CTCTGATCAAGTCAGTCAAG+AGG 0.573459 2_3:-15909805 Msa0280860:CDS
TTGATCTCAATGTTTGTACT+CGG 0.576687 2_3:+15909834 None:intergenic
TTTCCCTCTTAGATGCACTC+CGG 0.583189 2_3:-15910201 Msa0280860:CDS
TTTGTCGACAATTCATTAAG+GGG 0.583507 2_3:-15909873 Msa0280860:CDS
TCTGGAATTAAAGGATTAGG+GGG 0.587922 2_3:-15910131 Msa0280860:CDS
GACTGACTTGATCAGAGAAG+GGG 0.587939 2_3:+15909811 None:intergenic
TTCTGGAATTAAAGGATTAG+GGG 0.589816 2_3:-15910132 Msa0280860:CDS
CAAGGTGATCACCATAATGT+TGG 0.591086 2_3:-15909915 Msa0280860:CDS
TGTCGACAAAAGGTGTATTG+AGG 0.593503 2_3:+15909886 None:intergenic
AATAGAGATGGTATGAGTCA+AGG 0.612883 2_3:-15909933 Msa0280860:CDS
TACCCGGAGTGCATCTAAGA+GGG 0.617885 2_3:+15910198 None:intergenic
CAGACTCGACAAAATAGAGA+TGG 0.622886 2_3:-15909945 Msa0280860:CDS
CATCAGAAGTCCAATGATTG+GGG 0.623338 2_3:-15910080 Msa0280860:CDS
TACAAAAGGCGTGTTTCATG+CGG 0.631885 2_3:-15910162 Msa0280860:CDS
TTGTATTTCTCGCAGCACAA+AGG 0.634173 2_3:-15909663 Msa0280860:intron
CCACGATGGCCTAAAATCGA+CGG 0.634519 2_3:+15909984 None:intergenic
GAGTGATGATTAGATTCATG+TGG 0.665667 2_3:+15909519 None:intergenic
GTACTGCTACTGGATATACC+CGG 0.685345 2_3:+15910182 None:intergenic
ATAAACTGGAATGTTGTCTG+CGG 0.732217 2_3:+15909599 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGGAAACATAAATATTTT+GGG + chr2_3:15909919-15909938 None:intergenic 15.0%
!!! AATAAATGATTTGTCTATAA+AGG + chr2_3:15909947-15909966 None:intergenic 15.0%
!! TTAGTTTATGTAAAAATTTC+AGG - chr2_3:15910203-15910222 Msa0280860:CDS 15.0%
!!! TAAGGAAACATAAATATTTT+GGG + chr2_3:15909919-15909938 None:intergenic 15.0%
!!! AATAAATGATTTGTCTATAA+AGG + chr2_3:15909947-15909966 None:intergenic 15.0%
!! TTAGTTTATGTAAAAATTTC+AGG - chr2_3:15910203-15910222 Msa0280860:CDS 15.0%
!!! GTAAGGAAACATAAATATTT+TGG + chr2_3:15909920-15909939 None:intergenic 20.0%
!!! GTAAGGAAACATAAATATTT+TGG + chr2_3:15909920-15909939 None:intergenic 20.0%
!! TTTTGTCGACAATTCATTAA+GGG - chr2_3:15909776-15909795 Msa0280860:intron 25.0%
! TTGTCTATAAAGGAAAAGTA+AGG + chr2_3:15909937-15909956 None:intergenic 25.0%
!! TTTTGTCGACAATTCATTAA+GGG - chr2_3:15909776-15909795 Msa0280860:intron 25.0%
! TTGTCTATAAAGGAAAAGTA+AGG + chr2_3:15909937-15909956 None:intergenic 25.0%
!! ACTTCTGGAATTAAAGGATT+AGG - chr2_3:15909516-15909535 Msa0280860:three_prime_UTR 30.0%
!! CTTCTGGAATTAAAGGATTA+GGG - chr2_3:15909517-15909536 Msa0280860:three_prime_UTR 30.0%
!! TTCTGGAATTAAAGGATTAG+GGG - chr2_3:15909518-15909537 Msa0280860:three_prime_UTR 30.0%
CTTAATGAATTGTCGACAAA+AGG + chr2_3:15909777-15909796 None:intergenic 30.0%
! CTTTTGTCGACAATTCATTA+AGG - chr2_3:15909775-15909794 Msa0280860:intron 30.0%
TTTGTCGACAATTCATTAAG+GGG - chr2_3:15909777-15909796 Msa0280860:intron 30.0%
TTGATCTCAATGTTTGTACT+CGG + chr2_3:15909819-15909838 None:intergenic 30.0%
!! AATATTTTGGGTCAACAGAA+AGG + chr2_3:15909907-15909926 None:intergenic 30.0%
! GATAATGAGTTTGACTTTGA+TGG - chr2_3:15910023-15910042 Msa0280860:CDS 30.0%
TAACAGGATCTGTTATAAAC+TGG + chr2_3:15910068-15910087 None:intergenic 30.0%
TAGTTGCCAATGTAAATAGT+TGG - chr2_3:15910104-15910123 Msa0280860:CDS 30.0%
!! ACTTCTGGAATTAAAGGATT+AGG - chr2_3:15909516-15909535 Msa0280860:three_prime_UTR 30.0%
!! CTTCTGGAATTAAAGGATTA+GGG - chr2_3:15909517-15909536 Msa0280860:three_prime_UTR 30.0%
!! TTCTGGAATTAAAGGATTAG+GGG - chr2_3:15909518-15909537 Msa0280860:three_prime_UTR 30.0%
CTTAATGAATTGTCGACAAA+AGG + chr2_3:15909777-15909796 None:intergenic 30.0%
! CTTTTGTCGACAATTCATTA+AGG - chr2_3:15909775-15909794 Msa0280860:intron 30.0%
TTTGTCGACAATTCATTAAG+GGG - chr2_3:15909777-15909796 Msa0280860:intron 30.0%
TTGATCTCAATGTTTGTACT+CGG + chr2_3:15909819-15909838 None:intergenic 30.0%
!! AATATTTTGGGTCAACAGAA+AGG + chr2_3:15909907-15909926 None:intergenic 30.0%
! GATAATGAGTTTGACTTTGA+TGG - chr2_3:15910023-15910042 Msa0280860:CDS 30.0%
TAACAGGATCTGTTATAAAC+TGG + chr2_3:15910068-15910087 None:intergenic 30.0%
TAGTTGCCAATGTAAATAGT+TGG - chr2_3:15910104-15910123 Msa0280860:CDS 30.0%
ATATCCAGTAGCAGTACAAA+AGG - chr2_3:15909474-15909493 Msa0280860:three_prime_UTR 35.0%
!! GAAGCTACTTCTGGAATTAA+AGG - chr2_3:15909510-15909529 Msa0280860:three_prime_UTR 35.0%
! TCTGGAATTAAAGGATTAGG+GGG - chr2_3:15909519-15909538 Msa0280860:three_prime_UTR 35.0%
! ATTTGCTAATCCCCAATCAT+TGG + chr2_3:15909583-15909602 None:intergenic 35.0%
CAACATGCAAATCCTTTACA+AGG - chr2_3:15909627-15909646 Msa0280860:CDS 35.0%
AGATCAATGTTGCCTTGTAA+AGG + chr2_3:15909642-15909661 None:intergenic 35.0%
AATAGAGATGGTATGAGTCA+AGG - chr2_3:15909717-15909736 Msa0280860:intron 35.0%
ATAAACTGGAATGTTGTCTG+CGG + chr2_3:15910054-15910073 None:intergenic 35.0%
TGATTAGAAAGAGAGCTAAC+AGG + chr2_3:15910084-15910103 None:intergenic 35.0%
GGAACACCAACTATTTACAT+TGG + chr2_3:15910113-15910132 None:intergenic 35.0%
GAGTGATGATTAGATTCATG+TGG + chr2_3:15910134-15910153 None:intergenic 35.0%
ATATCCAGTAGCAGTACAAA+AGG - chr2_3:15909474-15909493 Msa0280860:three_prime_UTR 35.0%
!! GAAGCTACTTCTGGAATTAA+AGG - chr2_3:15909510-15909529 Msa0280860:three_prime_UTR 35.0%
! TCTGGAATTAAAGGATTAGG+GGG - chr2_3:15909519-15909538 Msa0280860:three_prime_UTR 35.0%
! ATTTGCTAATCCCCAATCAT+TGG + chr2_3:15909583-15909602 None:intergenic 35.0%
CAACATGCAAATCCTTTACA+AGG - chr2_3:15909627-15909646 Msa0280860:CDS 35.0%
AGATCAATGTTGCCTTGTAA+AGG + chr2_3:15909642-15909661 None:intergenic 35.0%
AATAGAGATGGTATGAGTCA+AGG - chr2_3:15909717-15909736 Msa0280860:intron 35.0%
ATAAACTGGAATGTTGTCTG+CGG + chr2_3:15910054-15910073 None:intergenic 35.0%
TGATTAGAAAGAGAGCTAAC+AGG + chr2_3:15910084-15910103 None:intergenic 35.0%
GGAACACCAACTATTTACAT+TGG + chr2_3:15910113-15910132 None:intergenic 35.0%
GAGTGATGATTAGATTCATG+TGG + chr2_3:15910134-15910153 None:intergenic 35.0%
! TACAAAAGGCGTGTTTCATG+CGG - chr2_3:15909488-15909507 Msa0280860:three_prime_UTR 40.0%
TCCATCAGAAGTCCAATGAT+TGG - chr2_3:15909568-15909587 Msa0280860:exon 40.0%
CCATCAGAAGTCCAATGATT+GGG - chr2_3:15909569-15909588 Msa0280860:exon 40.0%
CATCAGAAGTCCAATGATTG+GGG - chr2_3:15909570-15909589 Msa0280860:exon 40.0%
! GATTGGGGATTAGCAAATAC+AGG - chr2_3:15909585-15909604 Msa0280860:CDS 40.0%
!! GTTGAGAAGCATCATATGTC+AGG + chr2_3:15909611-15909630 None:intergenic 40.0%
! CTCTATTTTGTCGAGTCTGT+TGG + chr2_3:15909704-15909723 None:intergenic 40.0%
CAGACTCGACAAAATAGAGA+TGG - chr2_3:15909705-15909724 Msa0280860:intron 40.0%
CAAGGTGATCACCATAATGT+TGG - chr2_3:15909735-15909754 Msa0280860:intron 40.0%
!! TGAGGTGTTGACCAACATTA+TGG + chr2_3:15909749-15909768 None:intergenic 40.0%
! TGTCGACAAAAGGTGTATTG+AGG + chr2_3:15909767-15909786 None:intergenic 40.0%
!! TGACTGACTTGATCAGAGAA+GGG + chr2_3:15909843-15909862 None:intergenic 40.0%
!! TTGACTGACTTGATCAGAGA+AGG + chr2_3:15909844-15909863 None:intergenic 40.0%
TTGTATTTCTCGCAGCACAA+AGG - chr2_3:15909987-15910006 Msa0280860:CDS 40.0%
! TACAAAAGGCGTGTTTCATG+CGG - chr2_3:15909488-15909507 Msa0280860:three_prime_UTR 40.0%
TCCATCAGAAGTCCAATGAT+TGG - chr2_3:15909568-15909587 Msa0280860:exon 40.0%
CCATCAGAAGTCCAATGATT+GGG - chr2_3:15909569-15909588 Msa0280860:exon 40.0%
CATCAGAAGTCCAATGATTG+GGG - chr2_3:15909570-15909589 Msa0280860:exon 40.0%
! GATTGGGGATTAGCAAATAC+AGG - chr2_3:15909585-15909604 Msa0280860:CDS 40.0%
!! GTTGAGAAGCATCATATGTC+AGG + chr2_3:15909611-15909630 None:intergenic 40.0%
! CTCTATTTTGTCGAGTCTGT+TGG + chr2_3:15909704-15909723 None:intergenic 40.0%
CAGACTCGACAAAATAGAGA+TGG - chr2_3:15909705-15909724 Msa0280860:intron 40.0%
CAAGGTGATCACCATAATGT+TGG - chr2_3:15909735-15909754 Msa0280860:intron 40.0%
!! TGAGGTGTTGACCAACATTA+TGG + chr2_3:15909749-15909768 None:intergenic 40.0%
! TGTCGACAAAAGGTGTATTG+AGG + chr2_3:15909767-15909786 None:intergenic 40.0%
!! TGACTGACTTGATCAGAGAA+GGG + chr2_3:15909843-15909862 None:intergenic 40.0%
!! TTGACTGACTTGATCAGAGA+AGG + chr2_3:15909844-15909863 None:intergenic 40.0%
TTGTATTTCTCGCAGCACAA+AGG - chr2_3:15909987-15910006 Msa0280860:CDS 40.0%
TTTCCCTCTTAGATGCACTC+CGG - chr2_3:15909449-15909468 Msa0280860:three_prime_UTR 45.0%
GTACTGCTACTGGATATACC+CGG + chr2_3:15909471-15909490 None:intergenic 45.0%
TTTCATGCGGAAGCTACTTC+TGG - chr2_3:15909501-15909520 Msa0280860:three_prime_UTR 45.0%
CCCAATCATTGGACTTCTGA+TGG + chr2_3:15909572-15909591 None:intergenic 45.0%
GGATAGAGGAAAAACCACGA+TGG + chr2_3:15909683-15909702 None:intergenic 45.0%
TTGTCGAGTCTGTTGGATAG+AGG + chr2_3:15909697-15909716 None:intergenic 45.0%
!! GACTGACTTGATCAGAGAAG+GGG + chr2_3:15909842-15909861 None:intergenic 45.0%
CTCTGATCAAGTCAGTCAAG+AGG - chr2_3:15909845-15909864 Msa0280860:CDS 45.0%
! CATGAGCGTATTTCTGAACC+AGG - chr2_3:15909872-15909891 Msa0280860:CDS 45.0%
! AGCGTATTTCTGAACCAGGT+TGG - chr2_3:15909876-15909895 Msa0280860:CDS 45.0%
ACAGAAAGGAAGAACCAACC+TGG + chr2_3:15909893-15909912 None:intergenic 45.0%
TTTCCCTCTTAGATGCACTC+CGG - chr2_3:15909449-15909468 Msa0280860:three_prime_UTR 45.0%
GTACTGCTACTGGATATACC+CGG + chr2_3:15909471-15909490 None:intergenic 45.0%
TTTCATGCGGAAGCTACTTC+TGG - chr2_3:15909501-15909520 Msa0280860:three_prime_UTR 45.0%
CCCAATCATTGGACTTCTGA+TGG + chr2_3:15909572-15909591 None:intergenic 45.0%
GGATAGAGGAAAAACCACGA+TGG + chr2_3:15909683-15909702 None:intergenic 45.0%
TTGTCGAGTCTGTTGGATAG+AGG + chr2_3:15909697-15909716 None:intergenic 45.0%
!! GACTGACTTGATCAGAGAAG+GGG + chr2_3:15909842-15909861 None:intergenic 45.0%
CTCTGATCAAGTCAGTCAAG+AGG - chr2_3:15909845-15909864 Msa0280860:CDS 45.0%
! CATGAGCGTATTTCTGAACC+AGG - chr2_3:15909872-15909891 Msa0280860:CDS 45.0%
! AGCGTATTTCTGAACCAGGT+TGG - chr2_3:15909876-15909895 Msa0280860:CDS 45.0%
ACAGAAAGGAAGAACCAACC+TGG + chr2_3:15909893-15909912 None:intergenic 45.0%
TTCCCTCTTAGATGCACTCC+GGG - chr2_3:15909450-15909469 Msa0280860:three_prime_UTR 50.0%
TACCCGGAGTGCATCTAAGA+GGG + chr2_3:15909455-15909474 None:intergenic 50.0%
ATACCCGGAGTGCATCTAAG+AGG + chr2_3:15909456-15909475 None:intergenic 50.0%
! CACGCCTTTTGTACTGCTAC+TGG + chr2_3:15909481-15909500 None:intergenic 50.0%
!!! GATCTCTCGCCGTCGATTTT+AGG - chr2_3:15909657-15909676 Msa0280860:intron 50.0%
CCACGATGGCCTAAAATCGA+CGG + chr2_3:15909669-15909688 None:intergenic 50.0%
TTCCCTCTTAGATGCACTCC+GGG - chr2_3:15909450-15909469 Msa0280860:three_prime_UTR 50.0%
TACCCGGAGTGCATCTAAGA+GGG + chr2_3:15909455-15909474 None:intergenic 50.0%
ATACCCGGAGTGCATCTAAG+AGG + chr2_3:15909456-15909475 None:intergenic 50.0%
! CACGCCTTTTGTACTGCTAC+TGG + chr2_3:15909481-15909500 None:intergenic 50.0%
!!! GATCTCTCGCCGTCGATTTT+AGG - chr2_3:15909657-15909676 Msa0280860:intron 50.0%
CCACGATGGCCTAAAATCGA+CGG + chr2_3:15909669-15909688 None:intergenic 50.0%
!!! CCGTCGATTTTAGGCCATCG+TGG - chr2_3:15909666-15909685 Msa0280860:intron 55.0%
! TTCTCGCAGCACAAAGGCGT+TGG - chr2_3:15909993-15910012 Msa0280860:CDS 55.0%
!!! CCGTCGATTTTAGGCCATCG+TGG - chr2_3:15909666-15909685 Msa0280860:intron 55.0%
! TTCTCGCAGCACAAAGGCGT+TGG - chr2_3:15909993-15910012 Msa0280860:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 15909444 15910228 15909444 ID=Msa0280860;Name=Msa0280860
chr2_3 mRNA 15909444 15910228 15909444 ID=Msa0280860-mRNA-1;Parent=Msa0280860;Name=Msa0280860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|140|178
chr2_3 exon 15909444 15909670 15909444 ID=Msa0280860-mRNA-1:exon:6622;Parent=Msa0280860-mRNA-1
chr2_3 exon 15909779 15910228 15909779 ID=Msa0280860-mRNA-1:exon:6621;Parent=Msa0280860-mRNA-1
chr2_3 CDS 15909779 15910228 15909779 ID=Msa0280860-mRNA-1:cds;Parent=Msa0280860-mRNA-1
chr2_3 CDS 15909584 15909670 15909584 ID=Msa0280860-mRNA-1:cds;Parent=Msa0280860-mRNA-1
chr2_3 three_prime_UTR 15909444 15909583 15909444 ID=Msa0280860-mRNA-1:three_prime_utr;Parent=Msa0280860-mRNA-1
Gene Sequence

>Msa0280860

AATAGTTTCCCTCTTAGATGCACTCCGGGTATATCCAGTAGCAGTACAAAAGGCGTGTTTCATGCGGAAGCTACTTCTGGAATTAAAGGATTAGGGGGATTTAGTTATGATGTTTATAATGAACTCCATCAGAAGTCCAATGATTGGGGATTAGCAAATACAGGCCTGACATATGATGCTTCTCAACATGCAAATCCTTTACAAGGCAACATTGATCTCTCGCCGTCGATTTTAGGCCATCGTGGTTTTTCCTCTATCCAACAGACTCGACAAAATAGAGATGGTATGAGTCAAGGTGATCACCATAATGTTGGTCAACACCTCAATACACCTTTTGTCGACAATTCATTAAGGGGTAAGAGTGAAAGAATTCCGAGTACAAACATTGAGATCAACCCCTTCTCTGATCAAGTCAGTCAAGAGGATCTCATGAGCGTATTTCTGAACCAGCACAAAGGCGTTGGACAAGTTGATAATGAGTTTGACTTTGATGGATATTCCGCAGACAACATTCCAGTTTATAACAGATCCTGTTAG

Protein sequence

>Msa0280860

NSFPLRCTPGISSSSTKGVFHAEATSGIKGLGGFSYDVYNELHQKSNDWGLANTGLTYDASQHANPLQGNIDLSPSILGHRGFSSIQQTRQNRDGMSQGDHHNVGQHLNTPFVDNSLRGKSERIPSTNIEINPFSDQVSQEDLMSVFLNQHKGVGQVDNEFDFDGYSADNIPVYNRSC*