AlfalfaGEDB | Msa0282050

M. sativa cultivar zhongmu-4 / Msa0282050

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282050	GAU41275.1	71.591	176	48	1	1	174	1	176	2.29e-83	257

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282050	sp\|Q9FJK3\|AGL80_ARATH	54.321	162	71	2	1	161	1	160	1.16e-53	176

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282050	A0A2Z6NZ76	71.591	176	48	1	1	174	1	176	1.09e-83	257

TFs/TRs:

Gene ID	Type	Classification
Msa0282050	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0243590	Msa0282050	0.808115	3.628561e-50	-8.615850e-47
Msa0282050	Msa0282110	0.916242	2.254543e-85	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282050	MtrunA17_Chr8g0351751	69.730	185	47	3	1	177	1	184	1.01e-84	250
Msa0282050	MtrunA17_Chr2g0296831	94.262	122	7	0	1	122	1	122	4.03e-75	221
Msa0282050	MtrunA17_Chr4g0014031	66.111	180	56	2	1	176	1	179	5.95e-74	222
Msa0282050	MtrunA17_Chr4g0014321	64.815	162	57	0	1	162	1	162	2.44e-71	215
Msa0282050	MtrunA17_Chr3g0107381	62.821	156	57	1	1	156	1	155	6.78e-71	214
Msa0282050	MtrunA17_Chr3g0110131	65.000	160	55	1	1	160	1	159	1.62e-69	211
Msa0282050	MtrunA17_Chr4g0013041	65.584	154	51	1	1	154	1	152	1.03e-67	205
Msa0282050	MtrunA17_Chr4g0014131	63.333	180	61	2	1	176	1	179	9.18e-65	199
Msa0282050	MtrunA17_Chr4g0014121	69.677	155	47	0	1	155	29	183	1.49e-63	194
Msa0282050	MtrunA17_Chr3g0107331	50.943	159	77	1	3	161	2	159	1.12e-55	173
Msa0282050	MtrunA17_Chr3g0107351	46.203	158	84	1	3	160	2	158	4.21e-49	156
Msa0282050	MtrunA17_Chr3g0107361	46.835	158	83	1	3	160	2	158	8.33e-48	153
Msa0282050	MtrunA17_Chr3g0107341	45.570	158	85	1	3	160	2	158	1.71e-47	152
Msa0282050	MtrunA17_Chr3g0107461	41.935	155	90	0	1	155	1	155	1.99e-43	142
Msa0282050	MtrunA17_Chr2g0283941	43.038	158	90	0	1	158	1	158	6.15e-43	143
Msa0282050	MtrunA17_Chr5g0432691	41.875	160	92	1	1	160	1	159	9.10e-43	140
Msa0282050	MtrunA17_Chr2g0296851	80.412	97	19	0	74	170	1	97	5.72e-41	134
Msa0282050	MtrunA17_Chr5g0420351	43.226	155	86	2	1	155	1	153	1.12e-40	135
Msa0282050	MtrunA17_Chr1g0197261	39.873	158	93	2	1	158	1	156	2.12e-38	129
Msa0282050	MtrunA17_Chr4g0031421	42.969	128	73	0	29	156	1	128	1.30e-36	124
Msa0282050	MtrunA17_Chr7g0218151	44.156	154	84	2	1	154	1	152	3.80e-36	123
Msa0282050	MtrunA17_Chr1g0188661	43.506	154	85	2	1	154	1	152	3.28e-35	121
Msa0282050	MtrunA17_Chr1g0197321	36.709	158	99	1	1	158	1	157	3.35e-35	121
Msa0282050	MtrunA17_Chr1g0197461	38.462	156	94	2	3	158	2	155	3.99e-35	120
Msa0282050	MtrunA17_Chr1g0188681	39.610	154	91	2	1	154	1	152	7.79e-35	120
Msa0282050	MtrunA17_Chr3g0105791	41.085	129	76	0	29	157	1	129	1.05e-33	117
Msa0282050	MtrunA17_Chr1g0193311	41.290	155	89	2	1	155	1	153	1.91e-33	116
Msa0282050	MtrunA17_Chr4g0012761	41.558	154	88	2	1	154	1	152	2.22e-33	116
Msa0282050	MtrunA17_Chr1g0188651	42.857	154	84	2	1	154	1	150	5.99e-33	115
Msa0282050	MtrunA17_Chr3g0106421	44.094	127	71	0	29	155	1	127	6.29e-33	117
Msa0282050	MtrunA17_Chr2g0296861	36.774	155	97	1	1	155	1	154	7.45e-27	99.8
Msa0282050	MtrunA17_Chr3g0106501	33.846	130	86	0	29	158	1	130	4.76e-25	95.1
Msa0282050	MtrunA17_Chr4g0008701	37.879	132	79	1	1	129	1	132	1.72e-21	90.9
Msa0282050	MtrunA17_Chr7g0267601	37.121	132	80	1	1	129	1	132	1.96e-21	90.5
Msa0282050	MtrunA17_Chr3g0128721	34.646	127	83	0	3	129	6	132	2.15e-21	90.5
Msa0282050	MtrunA17_Chr6g0457851	30.357	112	71	3	1	106	11	121	9.30e-12	62.4
Msa0282050	MtrunA17_Chr1g0212151	25.180	139	87	5	1	127	1	134	9.86e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0282050	AT5G48670.1	54.321	162	71	2	1	161	1	160	1.18e-54	176
Msa0282050	AT5G26630.1	47.305	167	87	1	1	167	1	166	7.27e-45	148
Msa0282050	AT1G65330.1	40.000	180	107	1	3	182	2	180	1.25e-39	136
Msa0282050	AT1G65300.1	41.818	165	95	1	3	167	2	165	1.68e-39	136
Msa0282050	AT5G26650.1	43.038	158	89	1	4	161	2	158	2.54e-39	137
Msa0282050	AT5G27960.1	43.038	158	89	1	4	161	2	158	4.16e-39	136
Msa0282050	AT1G31630.1	34.302	172	111	2	3	174	2	171	2.14e-33	121
Msa0282050	AT3G05860.2	39.024	164	95	2	1	162	1	161	9.22e-33	116
Msa0282050	AT3G05860.3	39.024	164	95	2	1	162	1	161	6.48e-32	115
Msa0282050	AT3G05860.1	39.024	164	95	2	1	162	1	161	7.25e-32	115
Msa0282050	AT5G27810.1	51.613	93	45	0	29	121	1	93	1.07e-31	111
Msa0282050	AT1G31640.1	38.411	151	91	2	3	153	2	150	2.76e-30	115
Msa0282050	AT5G26580.1	36.025	161	88	2	1	161	1	146	9.37e-28	107
Msa0282050	AT2G28700.1	37.681	138	84	1	1	136	1	138	7.63e-27	104
Msa0282050	AT1G22590.2	29.448	163	110	2	1	163	1	158	1.15e-20	84.3
Msa0282050	AT5G06500.1	31.818	154	103	1	1	154	1	152	3.07e-20	85.1
Msa0282050	AT2G40210.1	37.795	127	79	0	1	127	1	127	3.52e-19	84.0
Msa0282050	AT5G55690.1	39.362	94	50	2	1	87	1	94	6.60e-13	65.9
Msa0282050	AT5G55690.2	39.362	94	50	2	1	87	1	94	6.60e-13	65.9
Msa0282050	AT5G58890.1	40.179	112	58	4	1	105	1	110	2.93e-12	63.9
Msa0282050	AT2G15660.1	26.562	128	90	2	38	163	31	156	6.10e-12	62.8

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCAATATCCTTCAAATTT+TGG	0.124846	2_3:+17755342	None:intergenic
TTCTTGTTCTTCTCTAATTC+AGG	0.242401	2_3:+17755551	None:intergenic
ATGAACCTCAACCTCATATT+TGG	0.258532	2_3:-17755622	Msa0282050:CDS
CACAATCATGACTAGGAAAA+AGG	0.298378	2_3:-17755780	None:intergenic
TGAAGCATGTGCTATAGTTC+AGG	0.354873	2_3:-17755651	Msa0282050:CDS
TTGTTGGTGGTGGTGTCATT+TGG	0.382506	2_3:+17755245	None:intergenic
GTTGATTAACCAAAATTTGA+AGG	0.422385	2_3:-17755351	Msa0282050:CDS
TTTGCATCATGAATGTCTCT+TGG	0.425971	2_3:+17755519	None:intergenic
GAAGGATATTGATAGAAGAT+TGG	0.427774	2_3:-17755333	Msa0282050:CDS
TCAAGTCATGACTACTCAAT+TGG	0.431051	2_3:-17755282	Msa0282050:CDS
ATATTTAATCTATAAATTGT+TGG	0.434030	2_3:+17755229	None:intergenic
ATACGGTGCTCTCAAAATTC+AGG	0.449943	2_3:-17755580	Msa0282050:CDS
TTGGAAGAAGTGGATAATAA+TGG	0.455688	2_3:-17755314	Msa0282050:CDS
GATGAATAATTTATTGAAGA+AGG	0.469514	2_3:-17755702	Msa0282050:CDS
GGAAAGCAACCTATAAGAAA+AGG	0.485933	2_3:-17755724	Msa0282050:CDS
TTGATTTGAATGACCTTGCT+TGG	0.489187	2_3:-17755373	Msa0282050:CDS
CTCATATTTGGCCATCACCA+TGG	0.499740	2_3:-17755610	Msa0282050:CDS
TCATATTTGGCCATCACCAT+GGG	0.503042	2_3:-17755609	Msa0282050:CDS
GATGAACTAAGCACGCTTTG+TGG	0.506861	2_3:-17755677	Msa0282050:CDS
GAAGCATGTGCTATAGTTCA+GGG	0.519428	2_3:-17755650	Msa0282050:CDS
TTTAATCTATAAATTGTTGG+TGG	0.522214	2_3:+17755232	None:intergenic
TGATAGAAGATTGGAAGAAG+TGG	0.523326	2_3:-17755324	Msa0282050:CDS
AATCTATAAATTGTTGGTGG+TGG	0.528345	2_3:+17755235	None:intergenic
ATCACCATGGGGAGTTCATA+CGG	0.531278	2_3:-17755597	Msa0282050:CDS
CATGGTGATGGCCAAATATG+AGG	0.531321	2_3:+17755611	None:intergenic
TTTGGTTAATCAACCAAGCA+AGG	0.538786	2_3:+17755360	None:intergenic
AGAGACATTCATGATGCAAA+GGG	0.543276	2_3:-17755516	Msa0282050:CDS
TCATTGTCAATGACGCTGCT+CGG	0.556837	2_3:-17755745	Msa0282050:CDS
TGAGTAGTCATGACTTGACT+TGG	0.575659	2_3:+17755287	None:intergenic
AGCAGCGTCATTGACAATGA+AGG	0.575999	2_3:+17755748	None:intergenic
GTATGAACTCCCCATGGTGA+TGG	0.577592	2_3:+17755599	None:intergenic
AAGAGACATTCATGATGCAA+AGG	0.578602	2_3:-17755517	Msa0282050:CDS
CACAACTCACAATCATGACT+AGG	0.590575	2_3:-17755787	None:intergenic
GATGGCCAAATATGAGGTTG+AGG	0.620009	2_3:+17755617	None:intergenic
AAAACTAAGGAAGGGAAACA+GGG	0.623030	2_3:-17755468	Msa0282050:CDS
ATATGAGGTTGAGGTTCATG+AGG	0.635051	2_3:+17755626	None:intergenic
AGCACCGTATGAACTCCCCA+TGG	0.692559	2_3:+17755593	None:intergenic
CATATTTGGCCATCACCATG+GGG	0.723900	2_3:-17755608	Msa0282050:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTATTCATCCTTTTCTTAT+AGG	+	chr2_3:17755299-17755318	None:intergenic	20.0%
!!	GATGAATAATTTATTGAAGA+AGG	-	chr2_3:17755309-17755328	Msa0282050:CDS	20.0%
!!!	TATCAATATCCTTCAAATTT+TGG	+	chr2_3:17755672-17755691	None:intergenic	20.0%
!	AAAGTGAAAAAACTAAGGAA+GGG	-	chr2_3:17755535-17755554	Msa0282050:CDS	25.0%
!	GTTGATTAACCAAAATTTGA+AGG	-	chr2_3:17755660-17755679	Msa0282050:CDS	25.0%
	TTCTTGTTCTTCTCTAATTC+AGG	+	chr2_3:17755463-17755482	None:intergenic	30.0%
	AGGAGAAAGTGAAAAAACTA+AGG	-	chr2_3:17755530-17755549	Msa0282050:CDS	30.0%
	GAAAGTGAAAAAACTAAGGA+AGG	-	chr2_3:17755534-17755553	Msa0282050:CDS	30.0%
	GAAGGATATTGATAGAAGAT+TGG	-	chr2_3:17755678-17755697	Msa0282050:CDS	30.0%
	TTGGAAGAAGTGGATAATAA+TGG	-	chr2_3:17755697-17755716	Msa0282050:CDS	30.0%
	GGAAAGCAACCTATAAGAAA+AGG	-	chr2_3:17755287-17755306	Msa0282050:CDS	35.0%
	ATGAACCTCAACCTCATATT+TGG	-	chr2_3:17755389-17755408	Msa0282050:CDS	35.0%
	TTTGCATCATGAATGTCTCT+TGG	+	chr2_3:17755495-17755514	None:intergenic	35.0%
	AAGAGACATTCATGATGCAA+AGG	-	chr2_3:17755494-17755513	Msa0282050:CDS	35.0%
	AGAGACATTCATGATGCAAA+GGG	-	chr2_3:17755495-17755514	Msa0282050:CDS	35.0%
	AAAAACTAAGGAAGGGAAAC+AGG	-	chr2_3:17755542-17755561	Msa0282050:CDS	35.0%
	AAAACTAAGGAAGGGAAACA+GGG	-	chr2_3:17755543-17755562	Msa0282050:CDS	35.0%
!	TTGATTTGAATGACCTTGCT+TGG	-	chr2_3:17755638-17755657	Msa0282050:CDS	35.0%
	TTTGGTTAATCAACCAAGCA+AGG	+	chr2_3:17755654-17755673	None:intergenic	35.0%
	TGATAGAAGATTGGAAGAAG+TGG	-	chr2_3:17755687-17755706	Msa0282050:CDS	35.0%
	TCAAGTCATGACTACTCAAT+TGG	-	chr2_3:17755729-17755748	Msa0282050:CDS	35.0%
	TGAAGCATGTGCTATAGTTC+AGG	-	chr2_3:17755360-17755379	Msa0282050:CDS	40.0%
	GAAGCATGTGCTATAGTTCA+GGG	-	chr2_3:17755361-17755380	Msa0282050:CDS	40.0%
	ATATGAGGTTGAGGTTCATG+AGG	+	chr2_3:17755388-17755407	None:intergenic	40.0%
	TCATATTTGGCCATCACCAT+GGG	-	chr2_3:17755402-17755421	Msa0282050:CDS	40.0%
	ATACGGTGCTCTCAAAATTC+AGG	-	chr2_3:17755431-17755450	Msa0282050:CDS	40.0%
!!	CATGATGCAAAGGGTTTTGA+AGG	-	chr2_3:17755504-17755523	Msa0282050:CDS	40.0%
	TGAGTAGTCATGACTTGACT+TGG	+	chr2_3:17755727-17755746	None:intergenic	40.0%
	AGCAGCGTCATTGACAATGA+AGG	+	chr2_3:17755266-17755285	None:intergenic	45.0%
	TCATTGTCAATGACGCTGCT+CGG	-	chr2_3:17755266-17755285	Msa0282050:CDS	45.0%
	GATGAACTAAGCACGCTTTG+TGG	-	chr2_3:17755334-17755353	Msa0282050:CDS	45.0%
	GATGGCCAAATATGAGGTTG+AGG	+	chr2_3:17755397-17755416	None:intergenic	45.0%
!!	CATGGTGATGGCCAAATATG+AGG	+	chr2_3:17755403-17755422	None:intergenic	45.0%
	CTCATATTTGGCCATCACCA+TGG	-	chr2_3:17755401-17755420	Msa0282050:CDS	45.0%
	CATATTTGGCCATCACCATG+GGG	-	chr2_3:17755403-17755422	Msa0282050:CDS	45.0%
	ATCACCATGGGGAGTTCATA+CGG	-	chr2_3:17755414-17755433	Msa0282050:CDS	45.0%
!!!	GCAAAGGGTTTTGAAGGCTA+AGG	-	chr2_3:17755510-17755529	Msa0282050:CDS	45.0%
!!	TTGTTGGTGGTGGTGTCATT+TGG	+	chr2_3:17755769-17755788	None:intergenic	45.0%
	GTATGAACTCCCCATGGTGA+TGG	+	chr2_3:17755415-17755434	None:intergenic	50.0%
	AGCACCGTATGAACTCCCCA+TGG	+	chr2_3:17755421-17755440	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	17755238	17755795	17755238	ID=Msa0282050;Name=Msa0282050
chr2_3	mRNA	17755238	17755795	17755238	ID=Msa0282050-mRNA-1;Parent=Msa0282050;Name=Msa0282050-mRNA-1;_AED=0.27;_eAED=0.30;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|185
chr2_3	exon	17755238	17755795	17755238	ID=Msa0282050-mRNA-1:exon:7309;Parent=Msa0282050-mRNA-1
chr2_3	CDS	17755238	17755795	17755238	ID=Msa0282050-mRNA-1:cds;Parent=Msa0282050-mRNA-1

Gene Sequence

>Msa0282050

ATGACTAGGAAAAAGGTGAAACTCGCCTTCATTGTCAATGACGCTGCTCGGAAAGCAACCTATAAGAAAAGGATGAATAATTTATTGAAGAAGGTCGATGAACTAAGCACGCTTTGTGGTATTGAAGCATGTGCTATAGTTCAGGGTCCTCATGAACCTCAACCTCATATTTGGCCATCACCATGGGGAGTTCATACGGTGCTCTCAAAATTCAGGACTATGCCTGAATTAGAGAAGAACAAGAAAATGATGAACCAAGAGACATTCATGATGCAAAGGGTTTTGAAGGCTAAGGAGAAAGTGAAAAAACTAAGGAAGGGAAACAGGGAGCAAGAGATGACAATGATCATGTTTCAATGTCTTAATGCTGAAAAAATTGTGCACGATGATATGTCAATGATTGATTTGAATGACCTTGCTTGGTTGATTAACCAAAATTTGAAGGATATTGATAGAAGATTGGAAGAAGTGGATAATAATGGTCATCCAAGTCAAGTCATGACTACTCAATTGGAGTTAAGTTCAACTCCAAATGACACCACCACCAACAATTTATAG

Protein sequence

>Msa0282050

MTRKKVKLAFIVNDAARKATYKKRMNNLLKKVDELSTLCGIEACAIVQGPHEPQPHIWPSPWGVHTVLSKFRTMPELEKNKKMMNQETFMMQRVLKAKEKVKKLRKGNREQEMTMIMFQCLNAEKIVHDDMSMIDLNDLAWLINQNLKDIDRRLEEVDNNGHPSQVMTTQLELSSTPNDTTTNNL*