AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0283380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTTACTTCTTCTCCTTCA+TGG 0.308459 2_3:-20534398 None:intergenic
TGCTGCTGCAACTTCTGTTT+CGG 0.361471 2_3:+20531681 None:intergenic
ATTGCCTTTAGGGTCCATAT+TGG 0.409955 2_3:+20531604 None:intergenic
TGCAGCGCTGGTTGCAATGA+AGG 0.439173 2_3:+20534356 None:intergenic
AGAAGGAGTCTACTATTTCA+AGG 0.491554 2_3:-20534300 Msa0283380:intron
AAGAAGAAGAACTGCCATGA+AGG 0.518565 2_3:+20534384 None:intergenic
CAGTGGTCTGAAAATTGAAA+AGG 0.521123 2_3:-20534335 Msa0283380:CDS
CATTGCAACCAGCGCTGCAG+TGG 0.543575 2_3:-20534352 Msa0283380:CDS
AAAGGACAGCAAGAAGAAGA+AGG 0.546280 2_3:-20534317 Msa0283380:CDS
AACGCCAATATGGACCCTAA+AGG 0.590616 2_3:-20531608 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGTTATTTATTTTTC+TGG - chr2_3:20533099-20533118 Msa0283380:intron 10.0%
!!! TTTTTTTTAATAGTATTTGA+TGG - chr2_3:20533505-20533524 Msa0283380:intron 10.0%
!!! TTTTTTTAATAGTATTTGAT+GGG - chr2_3:20533506-20533525 Msa0283380:intron 10.0%
!! ATAAATAAATTAAATTACTC+TGG - chr2_3:20533998-20534017 Msa0283380:intron 10.0%
!! ATAGAGAAAAGAAAAAAAAA+AGG + chr2_3:20531976-20531995 None:intergenic 15.0%
!!! TCTTATTGTTAATTTTCTTT+TGG - chr2_3:20532175-20532194 Msa0283380:intron 15.0%
!!! TAAGAATAATGGTTTTATAT+GGG + chr2_3:20532282-20532301 None:intergenic 15.0%
!!! ATAAGAATAATGGTTTTATA+TGG + chr2_3:20532283-20532302 None:intergenic 15.0%
!!! GTTAATTTAAACTTTAAAGT+TGG - chr2_3:20533616-20533635 Msa0283380:intron 15.0%
!! AAATTGATGAATGAATAATA+CGG + chr2_3:20533893-20533912 None:intergenic 15.0%
!! GTATCTGATAATAAGAATAA+TGG + chr2_3:20532293-20532312 None:intergenic 20.0%
!!! TATTTGTTCTTTTTTGTGTT+TGG - chr2_3:20533285-20533304 Msa0283380:intron 20.0%
!!! TGCATAAATATACTACATTT+TGG - chr2_3:20533334-20533353 Msa0283380:intron 20.0%
!! AATCAAATATGTTAGCTATT+TGG + chr2_3:20533362-20533381 None:intergenic 20.0%
!! AAATAGCTAACATATTTGAT+TGG - chr2_3:20533361-20533380 Msa0283380:intron 20.0%
!! AATAGCTAACATATTTGATT+GGG - chr2_3:20533362-20533381 Msa0283380:intron 20.0%
!! AATATATCTATCTGTTTGTT+GGG - chr2_3:20533557-20533576 Msa0283380:intron 20.0%
!!! TAATAACCAGATGATTTTAT+AGG - chr2_3:20533705-20533724 Msa0283380:intron 20.0%
!! TTATTCATTCATCAATTTCT+TGG - chr2_3:20533895-20533914 Msa0283380:intron 20.0%
!!! AGTAGTTTAACATTTTCTTA+AGG - chr2_3:20534089-20534108 Msa0283380:intron 20.0%
! CATTATCGTGAAAATTGAAA+AGG + chr2_3:20531754-20531773 None:intergenic 25.0%
! TGATGAGATATTTGCAAAAT+TGG - chr2_3:20532112-20532131 Msa0283380:intron 25.0%
! AGATACTCTTTCTTTATTGT+TGG - chr2_3:20532307-20532326 Msa0283380:intron 25.0%
!!! AATGAGATGATTTTCAAGAA+TGG - chr2_3:20532414-20532433 Msa0283380:intron 25.0%
! CACTCATAAAATAGAAATGA+CGG + chr2_3:20532516-20532535 None:intergenic 25.0%
! GTTGGGCTTATAATATATTT+CGG - chr2_3:20532836-20532855 Msa0283380:intron 25.0%
! AAAATTGAAGTCTATGTTTG+AGG - chr2_3:20533006-20533025 Msa0283380:intron 25.0%
! AAATTGAAGTCTATGTTTGA+GGG - chr2_3:20533007-20533026 Msa0283380:intron 25.0%
!!! TGTTAGACAAACTTTTTCTA+AGG - chr2_3:20533152-20533171 Msa0283380:intron 25.0%
!!! AATATGTTTTAGACGTTCAT+AGG + chr2_3:20533269-20533288 None:intergenic 25.0%
! TTAAAAAAAAAACAGCCACA+TGG + chr2_3:20533495-20533514 None:intergenic 25.0%
! GAATATATCTATCTGTTTGT+TGG - chr2_3:20533556-20533575 Msa0283380:intron 25.0%
! AGTTATGAAGAATGTGTATA+CGG + chr2_3:20533840-20533859 None:intergenic 25.0%
! TACACATTCTTCATAACTTA+AGG - chr2_3:20533842-20533861 Msa0283380:intron 25.0%
AGTCTACTATTTCAAGGTAT+GGG - chr2_3:20531709-20531728 Msa0283380:CDS 30.0%
AAATTGGTTGTAATCAGTGA+AGG - chr2_3:20532128-20532147 Msa0283380:intron 30.0%
AATTGGTTGTAATCAGTGAA+GGG - chr2_3:20532129-20532148 Msa0283380:intron 30.0%
!! TTGGATGTTCTGTTTCTATT+TGG - chr2_3:20532194-20532213 Msa0283380:intron 30.0%
! TCTTTATTGTTGGTCTAATG+AGG - chr2_3:20532317-20532336 Msa0283380:intron 30.0%
! AAACCTTGTCTAAGCTTTTT+CGG + chr2_3:20532348-20532367 None:intergenic 30.0%
! AAAAAGCTTAGACAAGGTTT+TGG - chr2_3:20532348-20532367 Msa0283380:intron 30.0%
!!! GACAAGGTTTTGGTTTAATT+TGG - chr2_3:20532358-20532377 Msa0283380:intron 30.0%
TAATTTGGCATGCTGTAATT+TGG - chr2_3:20532373-20532392 Msa0283380:intron 30.0%
AATTTGGCATGCTGTAATTT+GGG - chr2_3:20532374-20532393 Msa0283380:intron 30.0%
AGAAAATTAAGGTTCAGTCT+TGG - chr2_3:20532463-20532482 Msa0283380:intron 30.0%
GAAAATTAAGGTTCAGTCTT+GGG - chr2_3:20532464-20532483 Msa0283380:intron 30.0%
! AAATGACGGAATTTACAACT+GGG + chr2_3:20532502-20532521 None:intergenic 30.0%
! CGTCATTTCTATTTTATGAG+TGG - chr2_3:20532514-20532533 Msa0283380:intron 30.0%
! TTCTATTTTATGAGTGGTGT+TGG - chr2_3:20532520-20532539 Msa0283380:intron 30.0%
!! TCTAAGTTCTGATGTTGTTT+TGG + chr2_3:20532964-20532983 None:intergenic 30.0%
! TTTAGAATGTGCTAGCATTT+AGG - chr2_3:20533073-20533092 Msa0283380:intron 30.0%
GAGGTTTGTGAAAAACAAAT+TGG - chr2_3:20533195-20533214 Msa0283380:intron 30.0%
CTAACATATTTGATTGGGAT+TGG - chr2_3:20533367-20533386 Msa0283380:intron 30.0%
AGCATGTGTATCTTTACTTA+GGG + chr2_3:20533431-20533450 None:intergenic 30.0%
AAGCATGTGTATCTTTACTT+AGG + chr2_3:20533432-20533451 None:intergenic 30.0%
! ACACATGCTTTTACATTTGT+AGG - chr2_3:20533442-20533461 Msa0283380:intron 30.0%
TCTCTTCATTATGTATCTCT+TGG - chr2_3:20533594-20533613 Msa0283380:intron 30.0%
TTGGAACCTATAAAATCATC+TGG + chr2_3:20533714-20533733 None:intergenic 30.0%
! TAAAGGATGAGTCAAGATTT+TGG - chr2_3:20533764-20533783 Msa0283380:intron 30.0%
!!! GATTTTGGTTAAGAATTCCA+AGG - chr2_3:20533779-20533798 Msa0283380:intron 30.0%
!! TTAACAACCTTAAAAGCACT+TGG + chr2_3:20534130-20534149 None:intergenic 30.0%
!!! TTTTTTTGGTGGTTTAGGTA+TGG + chr2_3:20534377-20534396 None:intergenic 30.0%
CAGTGGTCTGAAAATTGAAA+AGG - chr2_3:20531668-20531687 Msa0283380:CDS 35.0%
AGAAGGAGTCTACTATTTCA+AGG - chr2_3:20531703-20531722 Msa0283380:CDS 35.0%
GAGTCTACTATTTCAAGGTA+TGG - chr2_3:20531708-20531727 Msa0283380:CDS 35.0%
ATTGGTTGTAATCAGTGAAG+GGG - chr2_3:20532130-20532149 Msa0283380:intron 35.0%
TGGGATGAACGAAATTCTAA+CGG + chr2_3:20532263-20532282 None:intergenic 35.0%
ATGCTGTAATTTGGGTGATT+TGG - chr2_3:20532382-20532401 Msa0283380:intron 35.0%
! TGTAATTTGGGTGATTTGGA+AGG - chr2_3:20532386-20532405 Msa0283380:intron 35.0%
! GGATATGGTGGAGAAAATTA+AGG - chr2_3:20532452-20532471 Msa0283380:intron 35.0%
! GAAATGACGGAATTTACAAC+TGG + chr2_3:20532503-20532522 None:intergenic 35.0%
TCGTCTTAGTTGTTATGTGT+TGG - chr2_3:20532577-20532596 Msa0283380:intron 35.0%
CGTCTTAGTTGTTATGTGTT+GGG - chr2_3:20532578-20532597 Msa0283380:intron 35.0%
!! CTGTTAGTTTGTTTCTGAGA+CGG - chr2_3:20532628-20532647 Msa0283380:intron 35.0%
!! TGTTAGTTTGTTTCTGAGAC+GGG - chr2_3:20532629-20532648 Msa0283380:intron 35.0%
!!! TTATAGGCAGCTGTTTTTAG+TGG - chr2_3:20532690-20532709 Msa0283380:intron 35.0%
! CTAATTGCTTTCTGGTGTAT+TGG - chr2_3:20532766-20532785 Msa0283380:intron 35.0%
!! GCTTTGTTTGGTACTTGAAT+TGG + chr2_3:20532897-20532916 None:intergenic 35.0%
TTGTGCAAAGAATGTGGTAA+GGG + chr2_3:20532936-20532955 None:intergenic 35.0%
ATTGTGCAAAGAATGTGGTA+AGG + chr2_3:20532937-20532956 None:intergenic 35.0%
GACAAATTGTGCAAAGAATG+TGG + chr2_3:20532942-20532961 None:intergenic 35.0%
ATTGAGGACCAAATTTGAGA+AGG - chr2_3:20533030-20533049 Msa0283380:intron 35.0%
CTACGTTTCCTTCTCAAATT+TGG + chr2_3:20533041-20533060 None:intergenic 35.0%
!! TTGTTTTTCACAAACCTCTC+TGG + chr2_3:20533193-20533212 None:intergenic 35.0%
GCATGTGTATCTTTACTTAG+GGG + chr2_3:20533430-20533449 None:intergenic 35.0%
TAGTATTTGATGGGACTGTT+TGG - chr2_3:20533515-20533534 Msa0283380:intron 35.0%
! ATCTGTTTGTTGGGTATGTT+TGG - chr2_3:20533566-20533585 Msa0283380:intron 35.0%
TTTGTTGGGTATGTTTGGTT+TGG - chr2_3:20533571-20533590 Msa0283380:intron 35.0%
!! CTTTCAAGTTCTCTGAGTTA+AGG + chr2_3:20533870-20533889 None:intergenic 35.0%
! TGAAATGCCAAGTGCTTTTA+AGG - chr2_3:20534120-20534139 Msa0283380:intron 35.0%
!! TTGAAGTGTGTACTGATTCT+AGG - chr2_3:20534184-20534203 Msa0283380:intron 35.0%
AAGAAGAAGAACTGCCATGA+AGG + chr2_3:20531622-20531641 None:intergenic 40.0%
AAAGGACAGCAAGAAGAAGA+AGG - chr2_3:20531686-20531705 Msa0283380:CDS 40.0%
GAAACACATAGATCAAGACG+AGG + chr2_3:20531873-20531892 None:intergenic 40.0%
!! GTGTTCTTGTGTTGCTTTCT+TGG - chr2_3:20531915-20531934 Msa0283380:intron 40.0%
!! TTCTTGTGTTGCTTTCTTGG+AGG - chr2_3:20531918-20531937 Msa0283380:intron 40.0%
GATGAACGAAATTCTAACGG+TGG + chr2_3:20532260-20532279 None:intergenic 40.0%
AGACCGAAAAAGCTTAGACA+AGG - chr2_3:20532342-20532361 Msa0283380:intron 40.0%
TGTAGCTGATGTAGAGGATA+TGG - chr2_3:20532437-20532456 Msa0283380:intron 40.0%
TTAAGGTTCAGTCTTGGGTT+TGG - chr2_3:20532469-20532488 Msa0283380:intron 40.0%
!! GTTAGTTTGTTTCTGAGACG+GGG - chr2_3:20532630-20532649 Msa0283380:intron 40.0%
!! TTAGTTTGTTTCTGAGACGG+GGG - chr2_3:20532631-20532650 Msa0283380:intron 40.0%
GCCTATAAACACAACCAACA+GGG + chr2_3:20532678-20532697 None:intergenic 40.0%
TGCCTATAAACACAACCAAC+AGG + chr2_3:20532679-20532698 None:intergenic 40.0%
CAAATCCACAACACCACAAA+CGG + chr2_3:20532745-20532764 None:intergenic 40.0%
GGATTTGGCTAATTGCTTTC+TGG - chr2_3:20532758-20532777 Msa0283380:intron 40.0%
TGCCTATCTTAAGTGGTGTT+GGG - chr2_3:20532819-20532838 Msa0283380:intron 40.0%
!! CAATCTCGGAAAGCTTTGTT+TGG + chr2_3:20532909-20532928 None:intergenic 40.0%
! GTGGTAAGGGTTTTCAATCT+CGG + chr2_3:20532923-20532942 None:intergenic 40.0%
AGTCTATGTTTGAGGGATTG+AGG - chr2_3:20533014-20533033 Msa0283380:intron 40.0%
!!! TTTTCTGGTTCGCTTTACTG+AGG - chr2_3:20533114-20533133 Msa0283380:intron 40.0%
!! TTTCTGGTTCGCTTTACTGA+GGG - chr2_3:20533115-20533134 Msa0283380:intron 40.0%
! ACTTTCAAAGTGTTCCAGAG+AGG - chr2_3:20533176-20533195 Msa0283380:intron 40.0%
TGAGCATGAGATAGAAGACT+TGG + chr2_3:20533245-20533264 None:intergenic 40.0%
GCTTACTTATTCTCTCCATG+TGG - chr2_3:20533477-20533496 Msa0283380:intron 40.0%
TAGTTGTCATGTTTGCTCCT+TGG + chr2_3:20533799-20533818 None:intergenic 40.0%
AATGTGTATACGGTTCACTG+AGG + chr2_3:20533830-20533849 None:intergenic 40.0%
!!! TGGCGTTTTTTTGGTGGTTT+AGG + chr2_3:20534382-20534401 None:intergenic 40.0%
!!! CCATATTGGCGTTTTTTTGG+TGG + chr2_3:20534388-20534407 None:intergenic 40.0%
CCACCAAAAAAACGCCAATA+TGG - chr2_3:20534385-20534404 Msa0283380:exon 40.0%
!!! GGTCCATATTGGCGTTTTTT+TGG + chr2_3:20534391-20534410 None:intergenic 40.0%
GAACGAAATTCTAACGGTGG+CGG + chr2_3:20532257-20532276 None:intergenic 45.0%
GAATGGTGTAGCTGATGTAG+AGG - chr2_3:20532431-20532450 Msa0283380:intron 45.0%
AGCTGATGTAGAGGATATGG+TGG - chr2_3:20532440-20532459 Msa0283380:intron 45.0%
ACCGAATTAGACAATCCCCA+CGG + chr2_3:20532549-20532568 None:intergenic 45.0%
AAACACAACCAACAGGGCAA+GGG + chr2_3:20532672-20532691 None:intergenic 45.0%
TAAACACAACCAACAGGGCA+AGG + chr2_3:20532673-20532692 None:intergenic 45.0%
! GCCCTGTTGGTTGTGTTTAT+AGG - chr2_3:20532674-20532693 Msa0283380:intron 45.0%
!!! TAGGCAGCTGTTTTTAGTGG+TGG - chr2_3:20532693-20532712 Msa0283380:intron 45.0%
ACCACAAACGGTATGGAACA+GGG + chr2_3:20532733-20532752 None:intergenic 45.0%
!! CGTTTGTGGTGTTGTGGATT+TGG - chr2_3:20532743-20532762 Msa0283380:intron 45.0%
CCACTTAAGATAGGCAGCTA+GGG + chr2_3:20532815-20532834 None:intergenic 45.0%
ACCACTTAAGATAGGCAGCT+AGG + chr2_3:20532816-20532835 None:intergenic 45.0%
CTGCCTATCTTAAGTGGTGT+TGG - chr2_3:20532818-20532837 Msa0283380:intron 45.0%
AGCCCAACACCACTTAAGAT+AGG + chr2_3:20532824-20532843 None:intergenic 45.0%
!! TTCTGGTTCGCTTTACTGAG+GGG - chr2_3:20533116-20533135 Msa0283380:intron 45.0%
! AGGCGCTACATGAAAGGTTT+TGG + chr2_3:20533733-20533752 None:intergenic 45.0%
CTTTCATGTAGCGCCTTCAA+GGG - chr2_3:20533737-20533756 Msa0283380:intron 45.0%
TTTCATGTAGCGCCTTCAAG+GGG - chr2_3:20533738-20533757 Msa0283380:intron 45.0%
CATCCTTTAACACCCCTTGA+AGG + chr2_3:20533753-20533772 None:intergenic 45.0%
TGCTGCTGCAACTTCTGTTT+CGG + chr2_3:20534325-20534344 None:intergenic 45.0%
!!! ATTTTTTTTTTATTTCAATA+TGG + chr2_3:20532085-20532104 None:intergenic 5.0%
CCGAATTAGACAATCCCCAC+GGG + chr2_3:20532548-20532567 None:intergenic 50.0%
CCCGTGGGGATTGTCTAATT+CGG - chr2_3:20532545-20532564 Msa0283380:intron 50.0%
GCGTGCAAGTAGTCTGATGT+GGG - chr2_3:20532600-20532619 Msa0283380:intron 50.0%
CGTGCAAGTAGTCTGATGTG+GGG - chr2_3:20532601-20532620 Msa0283380:intron 50.0%
! TTCTTTTCCCCTTGCCCTGT+TGG - chr2_3:20532661-20532680 Msa0283380:intron 50.0%
AACACAACCAACAGGGCAAG+GGG + chr2_3:20532671-20532690 None:intergenic 50.0%
TCCCTGTTCCATACCGTTTG+TGG - chr2_3:20532729-20532748 Msa0283380:intron 50.0%
CACCACAAACGGTATGGAAC+AGG + chr2_3:20532734-20532753 None:intergenic 50.0%
CCACAACACCACAAACGGTA+TGG + chr2_3:20532740-20532759 None:intergenic 50.0%
! CCATACCGTTTGTGGTGTTG+TGG - chr2_3:20532737-20532756 Msa0283380:intron 50.0%
CCCTAGCTGCCTATCTTAAG+TGG - chr2_3:20532812-20532831 Msa0283380:intron 50.0%
!! CCTTGAAGGCGCTACATGAA+AGG + chr2_3:20533739-20533758 None:intergenic 50.0%
CCTTTCATGTAGCGCCTTCA+AGG - chr2_3:20533736-20533755 Msa0283380:intron 50.0%
! ATACGGTTCACTGAGGCATC+TGG + chr2_3:20533823-20533842 None:intergenic 50.0%
!! TGCAGCGCTGGTTGCAATGA+AGG + chr2_3:20531650-20531669 None:intergenic 55.0%
! TTTTCAGACCACTGCAGCGC+TGG + chr2_3:20531662-20531681 None:intergenic 55.0%
! ATGAGTGGTGTTGGAACCCG+TGG - chr2_3:20532529-20532548 Msa0283380:intron 55.0%
! TGAGTGGTGTTGGAACCCGT+GGG - chr2_3:20532530-20532549 Msa0283380:intron 55.0%
GGCGTGCAAGTAGTCTGATG+TGG - chr2_3:20532599-20532618 Msa0283380:intron 55.0%
!! GCGCCTTCAAGGGGTGTTAA+AGG - chr2_3:20533747-20533766 Msa0283380:intron 55.0%
CATTGCAACCAGCGCTGCAG+TGG - chr2_3:20531651-20531670 Msa0283380:CDS 60.0%
! GAGTGGTGTTGGAACCCGTG+GGG - chr2_3:20532531-20532550 Msa0283380:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 20531611 20534414 20531611 ID=Msa0283380;Name=Msa0283380
chr2_3 mRNA 20531611 20534414 20531611 ID=Msa0283380-mRNA-1;Parent=Msa0283380;Name=Msa0283380-mRNA-1;_AED=0.65;_eAED=0.65;_QI=13|0|0.5|0.5|1|1|2|0|72
chr2_3 exon 20531611 20531728 20531611 ID=Msa0283380-mRNA-1:exon:8133;Parent=Msa0283380-mRNA-1
chr2_3 exon 20534301 20534414 20534301 ID=Msa0283380-mRNA-1:exon:8132;Parent=Msa0283380-mRNA-1
chr2_3 five_prime_UTR 20534402 20534414 20534402 ID=Msa0283380-mRNA-1:five_prime_utr;Parent=Msa0283380-mRNA-1
chr2_3 CDS 20534301 20534401 20534301 ID=Msa0283380-mRNA-1:cds;Parent=Msa0283380-mRNA-1
chr2_3 CDS 20531611 20531728 20531611 ID=Msa0283380-mRNA-1:cds;Parent=Msa0283380-mRNA-1
Gene Sequence

>Msa0283380

ATGGCAGTTCTTCTTCTTCATCTCCTTCATTGCAACCAGCGCTGCAGTGGTCTGAAAATTGAAAAGGACAGCAAGAAGAAGAAGGAGTCTACTATTTCAAGTGTAGACAGCAAACAACGAAAGATGCCGAAACAGAAGTTGCAGCAGCATCATACAGCTGCTGTCATACAGCTTACTGCCATACCTAAACCACCAAAAAAACGCCAATATGGACCCTAA

Protein sequence

>Msa0283380

MAVLLLHLLHCNQRCSGLKIEKDSKKKKESTISSVDSKQRKMPKQKLQQHHTAAVIQLTAIPKPPKKRQYGP*