AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1243300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 7 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTTACTTCTTCTCTTTCA+TGG 0.301626 8_2:-28836572 Msa1243300:CDS
TGCTGCTGCAACTTCTGTTT+CGG 0.361471 8_2:+28833855 None:intergenic
TGCAGCGCTGGTTGCAATGA+AGG 0.439173 8_2:+28836530 None:intergenic
AGAAGGAGTCTACTATTTCA+AGG 0.491554 8_2:-28836474 Msa1243300:intron
CAGTGGTCTGAAAATTGAAA+AGG 0.521123 8_2:-28836509 Msa1243300:CDS
CATTGCAACCAGCGCTGCAG+TGG 0.543575 8_2:-28836526 Msa1243300:CDS
AAAGGACAGCAAGAAGAAGA+AGG 0.546280 8_2:-28836491 Msa1243300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTGTTATTTATTTTTC+TGG - chr8_2:28835436-28835455 Msa1243300:intron 10.0%
!!! TTTTTTTTAATAGTATTTGA+TGG - chr8_2:28835842-28835861 Msa1243300:intron 10.0%
!!! TTTTTTTAATAGTATTTGAT+GGG - chr8_2:28835843-28835862 Msa1243300:intron 10.0%
!! ATAAATAAATTAAATTACTC+TGG - chr8_2:28836334-28836353 Msa1243300:intron 10.0%
!! ATAGAGAAAAGAAAAAAAAA+AGG + chr8_2:28834312-28834331 None:intergenic 15.0%
!!! TCTTATTGTTAATTTTCTTT+TGG - chr8_2:28834510-28834529 Msa1243300:intron 15.0%
!!! TAAGAATAATGGTTTTATAT+GGG + chr8_2:28834617-28834636 None:intergenic 15.0%
!!! ATAAGAATAATGGTTTTATA+TGG + chr8_2:28834618-28834637 None:intergenic 15.0%
!! AAATTGATGAATGAATAATA+CGG + chr8_2:28836229-28836248 None:intergenic 15.0%
!! GTATCTGATAATAAGAATAA+TGG + chr8_2:28834628-28834647 None:intergenic 20.0%
!!! TATTTGTTCTTTTTTGTGTT+TGG - chr8_2:28835622-28835641 Msa1243300:intron 20.0%
!!! TGCATAAATATACTACATTT+TGG - chr8_2:28835671-28835690 Msa1243300:intron 20.0%
!! AATCAAATATGTTAGCTATT+TGG + chr8_2:28835699-28835718 None:intergenic 20.0%
!! AAATAGCTAACATATTTGAT+TGG - chr8_2:28835698-28835717 Msa1243300:intron 20.0%
!! AATAGCTAACATATTTGATT+GGG - chr8_2:28835699-28835718 Msa1243300:intron 20.0%
!! TTAAAAAAAAAACAGCCATA+TGG + chr8_2:28835832-28835851 None:intergenic 20.0%
!! AATATATCTATCTGTTTGTT+GGG - chr8_2:28835894-28835913 Msa1243300:intron 20.0%
!!! GGTTATTTAAACTTTAAAGT+TGG - chr8_2:28835952-28835971 Msa1243300:intron 20.0%
!!! TAATAACCAGATGATTTTAT+AGG - chr8_2:28836041-28836060 Msa1243300:intron 20.0%
!! TTATTCATTCATCAATTTCT+TGG - chr8_2:28836231-28836250 Msa1243300:intron 20.0%
!!! AGTAGTTTAACATTTTCTTA+AGG - chr8_2:28836425-28836444 Msa1243300:intron 20.0%
! AAGTGCAAAATTCATAAGTT+TGG + chr8_2:28833860-28833879 None:intergenic 25.0%
!! AACTTATGAATTTTGCACTT+TGG - chr8_2:28833860-28833879 Msa1243300:CDS 25.0%
!! ACTTATGAATTTTGCACTTT+GGG - chr8_2:28833861-28833880 Msa1243300:CDS 25.0%
! CATTATCGTGAAAATTGAAA+AGG + chr8_2:28834090-28834109 None:intergenic 25.0%
! TGATGAGATATTTGCAAAAT+TGG - chr8_2:28834447-28834466 Msa1243300:intron 25.0%
! AGATACTCTTTCTTTATTGT+TGG - chr8_2:28834642-28834661 Msa1243300:intron 25.0%
!!! AATGAGATGATTTTCAAGAA+TGG - chr8_2:28834749-28834768 Msa1243300:intron 25.0%
! CACTCATAAAATAGAAATGA+CGG + chr8_2:28834851-28834870 None:intergenic 25.0%
! GTTGGGCTTATAATATATTT+CGG - chr8_2:28835171-28835190 Msa1243300:intron 25.0%
! AAAATTGAAGTCTATGTTTG+AGG - chr8_2:28835343-28835362 Msa1243300:intron 25.0%
! AAATTGAAGTCTATGTTTGA+GGG - chr8_2:28835344-28835363 Msa1243300:intron 25.0%
!!! TGTTAGACAAACTTTTTCTA+AGG - chr8_2:28835489-28835508 Msa1243300:intron 25.0%
!!! AATATGTTTTAGACGTTCAT+AGG + chr8_2:28835606-28835625 None:intergenic 25.0%
! GAATATATCTATCTGTTTGT+TGG - chr8_2:28835893-28835912 Msa1243300:intron 25.0%
! AGTTATGAAGAATGTGTATA+CGG + chr8_2:28836176-28836195 None:intergenic 25.0%
! TACACATTCTTCATAACTTA+AGG - chr8_2:28836178-28836197 Msa1243300:intron 25.0%
TGCAAAATTCATAAGTTTGG+TGG + chr8_2:28833857-28833876 None:intergenic 30.0%
! CTTATGAATTTTGCACTTTG+GGG - chr8_2:28833862-28833881 Msa1243300:CDS 30.0%
AGTCTACTATTTCAAGGTAT+GGG - chr8_2:28834045-28834064 Msa1243300:intron 30.0%
AAATTGGTTGTAATCAGTGA+AGG - chr8_2:28834463-28834482 Msa1243300:intron 30.0%
AATTGGTTGTAATCAGTGAA+GGG - chr8_2:28834464-28834483 Msa1243300:intron 30.0%
!! TTGGATGTTCTGTTTCTATT+TGG - chr8_2:28834529-28834548 Msa1243300:intron 30.0%
! TCTTTATTGTTGGTCTAATG+AGG - chr8_2:28834652-28834671 Msa1243300:intron 30.0%
! AAACCTTGTCTAAGCTTTTT+CGG + chr8_2:28834683-28834702 None:intergenic 30.0%
! AAAAAGCTTAGACAAGGTTT+TGG - chr8_2:28834683-28834702 Msa1243300:intron 30.0%
!!! GACAAGGTTTTGGTTTAATT+TGG - chr8_2:28834693-28834712 Msa1243300:intron 30.0%
TAATTTGGCATGCTGTAATT+TGG - chr8_2:28834708-28834727 Msa1243300:intron 30.0%
AATTTGGCATGCTGTAATTT+GGG - chr8_2:28834709-28834728 Msa1243300:intron 30.0%
AGAAAATTAAGGTTCAGTCT+TGG - chr8_2:28834798-28834817 Msa1243300:intron 30.0%
GAAAATTAAGGTTCAGTCTT+GGG - chr8_2:28834799-28834818 Msa1243300:intron 30.0%
! AAATGACGGAATTTACAACT+GGG + chr8_2:28834837-28834856 None:intergenic 30.0%
! CGTCATTTCTATTTTATGAG+TGG - chr8_2:28834849-28834868 Msa1243300:intron 30.0%
! TTCTATTTTATGAGTGGTGT+TGG - chr8_2:28834855-28834874 Msa1243300:intron 30.0%
!! TCTAAGTTCTGATGTTGTTT+TGG + chr8_2:28835301-28835320 None:intergenic 30.0%
! TTTAGAATGTGCTAGCATTT+AGG - chr8_2:28835410-28835429 Msa1243300:intron 30.0%
GAGGTTTGTGAAAAACAAAT+TGG - chr8_2:28835532-28835551 Msa1243300:intron 30.0%
CTAACATATTTGATTGGGAT+TGG - chr8_2:28835704-28835723 Msa1243300:intron 30.0%
AGCATGTGTATCTTTACTTA+GGG + chr8_2:28835768-28835787 None:intergenic 30.0%
AAGCATGTGTATCTTTACTT+AGG + chr8_2:28835769-28835788 None:intergenic 30.0%
! ACACATGCTTTTACATTTGT+AGG - chr8_2:28835779-28835798 Msa1243300:intron 30.0%
TCTCTTCATTATGTATCTCT+TGG - chr8_2:28835931-28835950 Msa1243300:intron 30.0%
TTGGAACCTATAAAATCATC+TGG + chr8_2:28836050-28836069 None:intergenic 30.0%
! TAAAGGATGAGTCAAGATTT+TGG - chr8_2:28836100-28836119 Msa1243300:intron 30.0%
!!! GATTTTGGTTAAGAATTCCA+AGG - chr8_2:28836115-28836134 Msa1243300:intron 30.0%
!! TTAACAACCTTAAAAGCACT+TGG + chr8_2:28836466-28836485 None:intergenic 30.0%
ATGGAACAAGTAAGTTTGAC+TGG - chr8_2:28833816-28833835 Msa1243300:CDS 35.0%
TCGTTACTTCTTCTCTTTCA+TGG - chr8_2:28833941-28833960 Msa1243300:intron 35.0%
CAGTGGTCTGAAAATTGAAA+AGG - chr8_2:28834004-28834023 Msa1243300:intron 35.0%
AGAAGGAGTCTACTATTTCA+AGG - chr8_2:28834039-28834058 Msa1243300:intron 35.0%
GAGTCTACTATTTCAAGGTA+TGG - chr8_2:28834044-28834063 Msa1243300:intron 35.0%
ATTGGTTGTAATCAGTGAAG+GGG - chr8_2:28834465-28834484 Msa1243300:intron 35.0%
TGGGATGAACGAAATTCTAA+CGG + chr8_2:28834598-28834617 None:intergenic 35.0%
ATGCTGTAATTTGGGTGATT+TGG - chr8_2:28834717-28834736 Msa1243300:intron 35.0%
! TGTAATTTGGGTGATTTGGA+AGG - chr8_2:28834721-28834740 Msa1243300:intron 35.0%
TGTAACTGATGTAGAGGATA+TGG - chr8_2:28834772-28834791 Msa1243300:intron 35.0%
! GGATATGGTGGAGAAAATTA+AGG - chr8_2:28834787-28834806 Msa1243300:intron 35.0%
! GAAATGACGGAATTTACAAC+TGG + chr8_2:28834838-28834857 None:intergenic 35.0%
TCGTCTTAGTTGTTATGTGT+TGG - chr8_2:28834912-28834931 Msa1243300:intron 35.0%
CGTCTTAGTTGTTATGTGTT+GGG - chr8_2:28834913-28834932 Msa1243300:intron 35.0%
!! CTGTTAGTTTGTTTCTGAGA+CGG - chr8_2:28834963-28834982 Msa1243300:intron 35.0%
!! TGTTAGTTTGTTTCTGAGAC+GGG - chr8_2:28834964-28834983 Msa1243300:intron 35.0%
!!! TTATAGGCAGCTGTTTTTAG+TGG - chr8_2:28835025-28835044 Msa1243300:intron 35.0%
! CTAATTGCTTTCTGGTGTAT+TGG - chr8_2:28835101-28835120 Msa1243300:intron 35.0%
!! GCTTTGTTTGGTACTTGAAT+TGG + chr8_2:28835234-28835253 None:intergenic 35.0%
TTGTGCAAAGAATGTGGTAA+GGG + chr8_2:28835273-28835292 None:intergenic 35.0%
ATTGTGCAAAGAATGTGGTA+AGG + chr8_2:28835274-28835293 None:intergenic 35.0%
GACAAATTGTGCAAAGAATG+TGG + chr8_2:28835279-28835298 None:intergenic 35.0%
ATTGAGGACCAAATTTGAGA+AGG - chr8_2:28835367-28835386 Msa1243300:intron 35.0%
CTACGTTTCCTTCTCAAATT+TGG + chr8_2:28835378-28835397 None:intergenic 35.0%
!! TTGTTTTTCACAAACCTCTC+TGG + chr8_2:28835530-28835549 None:intergenic 35.0%
GCATGTGTATCTTTACTTAG+GGG + chr8_2:28835767-28835786 None:intergenic 35.0%
GCTTACTTATTCTCTCCATA+TGG - chr8_2:28835814-28835833 Msa1243300:intron 35.0%
TAGTATTTGATGGGACTGTT+TGG - chr8_2:28835852-28835871 Msa1243300:intron 35.0%
! ATCTGTTTGTTGGGTATGTT+TGG - chr8_2:28835903-28835922 Msa1243300:intron 35.0%
TTTGTTGGGTATGTTTGGTT+TGG - chr8_2:28835908-28835927 Msa1243300:intron 35.0%
!! CTTTCAAGTTCTCTGAGTTA+AGG + chr8_2:28836206-28836225 None:intergenic 35.0%
! TGAAATGCCAAGTGCTTTTA+AGG - chr8_2:28836456-28836475 Msa1243300:intron 35.0%
!! TTGAAGTGTGTACTGATTCT+AGG - chr8_2:28836520-28836539 Msa1243300:CDS 35.0%
AAAGGACAGCAAGAAGAAGA+AGG - chr8_2:28834022-28834041 Msa1243300:intron 40.0%
GAAACACATAGATCAAGACG+AGG + chr8_2:28834209-28834228 None:intergenic 40.0%
!! GTGTTCTTGTGTTGCTTTCT+TGG - chr8_2:28834251-28834270 Msa1243300:intron 40.0%
!! TTCTTGTGTTGCTTTCTTGG+AGG - chr8_2:28834254-28834273 Msa1243300:intron 40.0%
GATGAACGAAATTCTAACGG+TGG + chr8_2:28834595-28834614 None:intergenic 40.0%
AGACCGAAAAAGCTTAGACA+AGG - chr8_2:28834677-28834696 Msa1243300:intron 40.0%
GAATGGTGTAACTGATGTAG+AGG - chr8_2:28834766-28834785 Msa1243300:intron 40.0%
AACTGATGTAGAGGATATGG+TGG - chr8_2:28834775-28834794 Msa1243300:intron 40.0%
TTAAGGTTCAGTCTTGGGTT+TGG - chr8_2:28834804-28834823 Msa1243300:intron 40.0%
!! GTTAGTTTGTTTCTGAGACG+GGG - chr8_2:28834965-28834984 Msa1243300:intron 40.0%
!! TTAGTTTGTTTCTGAGACGG+GGG - chr8_2:28834966-28834985 Msa1243300:intron 40.0%
GCCTATAAACACAACCAACA+GGG + chr8_2:28835013-28835032 None:intergenic 40.0%
TGCCTATAAACACAACCAAC+AGG + chr8_2:28835014-28835033 None:intergenic 40.0%
CAAATCCACAAGACCACAAA+CGG + chr8_2:28835080-28835099 None:intergenic 40.0%
GGATTTGGCTAATTGCTTTC+TGG - chr8_2:28835093-28835112 Msa1243300:intron 40.0%
TGCCTATCTTAAGTGGTGTT+GGG - chr8_2:28835154-28835173 Msa1243300:intron 40.0%
!! CAATCTCGGAAAGCTTTGTT+TGG + chr8_2:28835246-28835265 None:intergenic 40.0%
! GTGGTAAGGGTTTTCAATCT+CGG + chr8_2:28835260-28835279 None:intergenic 40.0%
AGTCTATGTTTGAGGGATTG+AGG - chr8_2:28835351-28835370 Msa1243300:intron 40.0%
!!! TTTTCTGGTTCGCTTTACTG+AGG - chr8_2:28835451-28835470 Msa1243300:intron 40.0%
!! TTTCTGGTTCGCTTTACTGA+GGG - chr8_2:28835452-28835471 Msa1243300:intron 40.0%
! ACTTTCAAAGTGTTCCAGAG+AGG - chr8_2:28835513-28835532 Msa1243300:intron 40.0%
TGAGCATGAGATAGAAGACT+TGG + chr8_2:28835582-28835601 None:intergenic 40.0%
TAGTTGTCATGTTTGCTCCT+TGG + chr8_2:28836135-28836154 None:intergenic 40.0%
AATGTGTATACGGTTCACTG+AGG + chr8_2:28836166-28836185 None:intergenic 40.0%
GAACGAAATTCTAACGGTGG+CGG + chr8_2:28834592-28834611 None:intergenic 45.0%
ACCGAATTAGACAATCCCCA+CGG + chr8_2:28834884-28834903 None:intergenic 45.0%
AAACACAACCAACAGGGCAA+GGG + chr8_2:28835007-28835026 None:intergenic 45.0%
TAAACACAACCAACAGGGCA+AGG + chr8_2:28835008-28835027 None:intergenic 45.0%
! GCCCTGTTGGTTGTGTTTAT+AGG - chr8_2:28835009-28835028 Msa1243300:intron 45.0%
!!! TAGGCAGCTGTTTTTAGTGG+TGG - chr8_2:28835028-28835047 Msa1243300:intron 45.0%
ACCACAAACGGTATGGAACA+GGG + chr8_2:28835068-28835087 None:intergenic 45.0%
!! CGTTTGTGGTCTTGTGGATT+TGG - chr8_2:28835078-28835097 Msa1243300:intron 45.0%
CCACTTAAGATAGGCAGCTA+GGG + chr8_2:28835150-28835169 None:intergenic 45.0%
ACCACTTAAGATAGGCAGCT+AGG + chr8_2:28835151-28835170 None:intergenic 45.0%
CTGCCTATCTTAAGTGGTGT+TGG - chr8_2:28835153-28835172 Msa1243300:intron 45.0%
AGCCCAACACCACTTAAGAT+AGG + chr8_2:28835159-28835178 None:intergenic 45.0%
!! TTCTGGTTCGCTTTACTGAG+GGG - chr8_2:28835453-28835472 Msa1243300:intron 45.0%
! AGGCGCTACATGAAAGGTTT+TGG + chr8_2:28836069-28836088 None:intergenic 45.0%
CTTTCATGTAGCGCCTTCAA+GGG - chr8_2:28836073-28836092 Msa1243300:intron 45.0%
TTTCATGTAGCGCCTTCAAG+GGG - chr8_2:28836074-28836093 Msa1243300:intron 45.0%
CATCCTTTAACACCCCTTGA+AGG + chr8_2:28836089-28836108 None:intergenic 45.0%
TGCTGCTGCAACTTCTGTTT+CGG + chr8_2:28836661-28836680 None:intergenic 45.0%
!!! AATTTTTTTTTATTTCAATA+TGG + chr8_2:28834421-28834440 None:intergenic 5.0%
CCGAATTAGACAATCCCCAC+GGG + chr8_2:28834883-28834902 None:intergenic 50.0%
CCCGTGGGGATTGTCTAATT+CGG - chr8_2:28834880-28834899 Msa1243300:intron 50.0%
GCGTGCAAGTAGTCTGATGT+GGG - chr8_2:28834935-28834954 Msa1243300:intron 50.0%
CGTGCAAGTAGTCTGATGTG+GGG - chr8_2:28834936-28834955 Msa1243300:intron 50.0%
! TTCTTTTCCCCTTGCCCTGT+TGG - chr8_2:28834996-28835015 Msa1243300:intron 50.0%
AACACAACCAACAGGGCAAG+GGG + chr8_2:28835006-28835025 None:intergenic 50.0%
TCCCTGTTCCATACCGTTTG+TGG - chr8_2:28835064-28835083 Msa1243300:intron 50.0%
GACCACAAACGGTATGGAAC+AGG + chr8_2:28835069-28835088 None:intergenic 50.0%
CCACAAGACCACAAACGGTA+TGG + chr8_2:28835075-28835094 None:intergenic 50.0%
! CCATACCGTTTGTGGTCTTG+TGG - chr8_2:28835072-28835091 Msa1243300:intron 50.0%
CCCTAGCTGCCTATCTTAAG+TGG - chr8_2:28835147-28835166 Msa1243300:intron 50.0%
!! CCTTGAAGGCGCTACATGAA+AGG + chr8_2:28836075-28836094 None:intergenic 50.0%
CCTTTCATGTAGCGCCTTCA+AGG - chr8_2:28836072-28836091 Msa1243300:intron 50.0%
! ATACGGTTCACTGAGGCATC+TGG + chr8_2:28836159-28836178 None:intergenic 50.0%
!! TGCAGCGCTGGTTGCAATGA+AGG + chr8_2:28833986-28834005 None:intergenic 55.0%
! TTTTCAGACCACTGCAGCGC+TGG + chr8_2:28833998-28834017 None:intergenic 55.0%
! ATGAGTGGTGTTGGAACCCG+TGG - chr8_2:28834864-28834883 Msa1243300:intron 55.0%
! TGAGTGGTGTTGGAACCCGT+GGG - chr8_2:28834865-28834884 Msa1243300:intron 55.0%
GGCGTGCAAGTAGTCTGATG+TGG - chr8_2:28834934-28834953 Msa1243300:intron 55.0%
!! GCGCCTTCAAGGGGTGTTAA+AGG - chr8_2:28836083-28836102 Msa1243300:intron 55.0%
CATTGCAACCAGCGCTGCAG+TGG - chr8_2:28833987-28834006 Msa1243300:intron 60.0%
! GAGTGGTGTTGGAACCCGTG+GGG - chr8_2:28834866-28834885 Msa1243300:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 28833816 28836719 28833816 ID=Msa1243300;Name=Msa1243300
chr8_2 mRNA 28833816 28836719 28833816 ID=Msa1243300-mRNA-1;Parent=Msa1243300;Name=Msa1243300-mRNA-1;_AED=0.64;_eAED=0.64;_QI=0|0|0|0.33|1|1|3|0|74
chr8_2 exon 28836711 28836719 28836711 ID=Msa1243300-mRNA-1:exon:9985;Parent=Msa1243300-mRNA-1
chr8_2 exon 28836475 28836608 28836475 ID=Msa1243300-mRNA-1:exon:9984;Parent=Msa1243300-mRNA-1
chr8_2 exon 28833816 28833897 28833816 ID=Msa1243300-mRNA-1:exon:9983;Parent=Msa1243300-mRNA-1
chr8_2 CDS 28836711 28836719 28836711 ID=Msa1243300-mRNA-1:cds;Parent=Msa1243300-mRNA-1
chr8_2 CDS 28836475 28836608 28836475 ID=Msa1243300-mRNA-1:cds;Parent=Msa1243300-mRNA-1
chr8_2 CDS 28833816 28833897 28833816 ID=Msa1243300-mRNA-1:cds;Parent=Msa1243300-mRNA-1
Gene Sequence

>Msa1243300

ATGGAACAAAAAACAAATTTAAATCGTTACTTCTTCTCTTTCATGGCAGTTCTTCTTCTTCATCTCCTTCATTGCAACCAGCGCTGCAGTGGTCTGAAAATTGAAAAGGACAGCAAGAAGAAGAAGGAGTCTACTATTTCAAGACAGCAAACAACGAAAGATGCCGAAACAGAAGTTGCAGCAGCATCATACAGCTGCTGTCATACAGCTTACTGCCATACCTAA

Protein sequence

>Msa1243300

MEQKTNLNRYFFSFMAVLLLHLLHCNQRCSGLKIEKDSKKKKESTISRQQTTKDAETEVAAASYSCCHTAYCHT*