AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0287750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0287750 MtrunA17_Chr4g0037741 75.926 54 13 0 120 173 74 127 4.88e-21 89.4
Msa0287750 MtrunA17_Chr4g0037761 68.519 54 17 0 120 173 74 127 2.18e-18 82.0
Msa0287750 MtrunA17_Chr4g0037791 62.963 54 20 0 120 173 144 197 2.32e-16 77.8
Msa0287750 MtrunA17_Chr3g0125141 52.381 63 30 0 175 237 1 63 5.54e-14 65.9
Msa0287750 MtrunA17_Chr1g0197101 90.625 32 3 0 89 120 55 86 4.81e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0287750 AT3G18630.1 57.407 54 23 0 120 173 172 225 3.76e-14 72.0

Find 110 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCTATTCGATTTC+AGG 0.110623 2_3:-30942960 Msa0287750:CDS
CTCAAGTACATTCTTCAAAA+TGG 0.251262 2_3:+30942194 None:intergenic
GTTGAGTATTGTCAACTATT+TGG 0.270362 2_3:-30940143 Msa0287750:three_prime_UTR
AGATTAGGACACATTATATT+AGG 0.275665 2_3:-30942466 Msa0287750:CDS
GAGGAGGTTAAGAATAATAT+TGG 0.300498 2_3:-30942174 Msa0287750:CDS
TAAGAATAATATTGGTTGTA+AGG 0.355976 2_3:-30942166 Msa0287750:CDS
CCTTGTGAGCTTAGCTCAGC+TGG 0.366709 2_3:+30940192 None:intergenic
TTAAATCTATAAAATTGATA+TGG 0.366835 2_3:+30940494 None:intergenic
GATGCAGGGCAGCTGGATAC+AGG 0.367904 2_3:-30940676 Msa0287750:CDS
GAGCGCCTCGTACAAGAAAA+AGG 0.373216 2_3:+30940412 None:intergenic
TTGACTCCCCTTCATTCAGC+TGG 0.374906 2_3:+30940713 None:intergenic
GATGCACGGCAGTCGGATAC+AGG 0.375011 2_3:-30940631 Msa0287750:CDS
GCTTCACCAAGTCCACAATA+AGG 0.376161 2_3:+30942261 None:intergenic
TGAGCTTAGCTCAGCTGGTT+GGG 0.376711 2_3:+30940197 None:intergenic
CTATTCGATTTCAGGATTCT+CGG 0.377459 2_3:-30942952 Msa0287750:CDS
GAATGAGATGGTTGCGCAAT+CGG 0.381997 2_3:+30943018 None:intergenic
ATTCTTATGTAAGGAATAGT+TGG 0.392168 2_3:+30940378 None:intergenic
TGTTGCAATTACTATCTTAG+TGG 0.392904 2_3:-30940099 Msa0287750:three_prime_UTR
GCTATCATTCTCGGTTCCTC+AGG 0.399999 2_3:-30942232 Msa0287750:CDS
TGCATAAAATATGCAAGGTC+CGG 0.400962 2_3:+30940224 None:intergenic
TTAAGATTATGATCATGATT+AGG 0.410727 2_3:+30940559 None:intergenic
TAGGAACCTTATTGTGGACT+TGG 0.414406 2_3:-30942267 Msa0287750:CDS
ACCCATAGGTCTCCTACTAT+TGG 0.414819 2_3:+30940305 None:intergenic
GCAGCTGGATACAGGGCATC+TGG 0.424953 2_3:-30940668 Msa0287750:CDS
TATTCGATTTCAGGATTCTC+GGG 0.426645 2_3:-30942951 Msa0287750:CDS
TATTGGAAGGATTCTTGTCT+AGG 0.432089 2_3:+30940322 None:intergenic
GAATATTGATAAGACTTTGA+AGG 0.433586 2_3:-30942432 Msa0287750:CDS
TTGAATAAATTGTGCATGTT+GGG 0.441819 2_3:-30939990 Msa0287750:three_prime_UTR
TCACTTTGATCAATATTGTC+AGG 0.442772 2_3:-30942390 Msa0287750:intron
TTTCAGGATTCTCGGGAGAA+AGG 0.450201 2_3:-30942944 Msa0287750:CDS
TTCAGGATTCTCGGGAGAAA+GGG 0.455367 2_3:-30942943 Msa0287750:CDS
ACAAGAATCCTTCCAATAGT+AGG 0.455811 2_3:-30940317 Msa0287750:three_prime_UTR
GGAGCAAGATGCAGGGCAGC+TGG 0.459296 2_3:-30940683 Msa0287750:CDS
GAAAGGGTTCTGGTCAGAAA+AGG 0.459541 2_3:-30942927 Msa0287750:CDS
GTGAGCTTAGCTCAGCTGGT+TGG 0.459734 2_3:+30940196 None:intergenic
TTCCAATAGTAGGAGACCTA+TGG 0.459868 2_3:-30940307 Msa0287750:three_prime_UTR
AAGGGGTTCCCGTGAGGAAG+GGG 0.470151 2_3:-30942908 Msa0287750:CDS
GTTGAATAAATTGTGCATGT+TGG 0.475595 2_3:-30939991 Msa0287750:three_prime_UTR
AGCAATGGGGCTATCATTCT+CGG 0.477510 2_3:-30942241 Msa0287750:CDS
AATGCAGGGGAGCAAGATGC+AGG 0.477678 2_3:-30940691 Msa0287750:CDS
ATTCTCGGGAGAAAGGGTTC+TGG 0.479737 2_3:-30942937 Msa0287750:CDS
TTCTTATGTAAGGAATAGTT+GGG 0.482757 2_3:+30940379 None:intergenic
AAAGGGTTCTGGTCAGAAAA+GGG 0.485467 2_3:-30942926 Msa0287750:CDS
TGTGGACTTGGTGAAGCAAT+GGG 0.489096 2_3:-30942255 Msa0287750:CDS
ATTGGAAGGATTCTTGTCTA+GGG 0.489782 2_3:+30940323 None:intergenic
GCATAAAATATGCAAGGTCC+GGG 0.491454 2_3:+30940225 None:intergenic
AAAAGGGGTTCCCGTGAGGA+AGG 0.495867 2_3:-30942910 Msa0287750:CDS
GACGCAGACCAGCTGAATGA+AGG 0.498682 2_3:-30940721 Msa0287750:CDS
ATACAGGGCATCTGGATGTA+AGG 0.499007 2_3:-30940660 Msa0287750:CDS
GATGCAGTGCAGTTGGATCC+AGG 0.503877 2_3:-30940601 Msa0287750:CDS
GTATCCGACTGCCGTGCATC+CGG 0.506762 2_3:+30940634 None:intergenic
AGAGGAAGGATTCTTATGTA+AGG 0.509703 2_3:+30940369 None:intergenic
CAAGCGACTAAGAATCTACA+TGG 0.511335 2_3:-30940796 Msa0287750:CDS
AGTACATTCTTCAAAATGGA+TGG 0.513241 2_3:+30942198 None:intergenic
TTATTCAACATTCCTGTGAA+AGG 0.518920 2_3:+30940005 None:intergenic
AGTGGCCGGGGTTTGAACCC+CGG 0.520773 2_3:-30940243 Msa0287750:three_prime_UTR
GCATCTAGATGCAGTGCAGT+TGG 0.520962 2_3:-30940608 Msa0287750:CDS
TGGTCTGCGTCCCTCTGCCA+TGG 0.521247 2_3:+30940733 None:intergenic
TTCAACATTCCTGTGAAAGG+TGG 0.522986 2_3:+30940008 None:intergenic
GGTTGTAAGGTTCTACCTCA+TGG 0.523538 2_3:-30942153 Msa0287750:CDS
AATGAAGGGGAGTCAAATGC+AGG 0.523780 2_3:-30940706 Msa0287750:CDS
GATGGTTGCGCAATCGGAGT+AGG 0.526052 2_3:+30943024 None:intergenic
GGAGTAGGACGGTGGCCAAT+CGG 0.530563 2_3:+30943039 None:intergenic
GAGAGAGAGAGGAAGTCGGT+GGG 0.531856 2_3:+30942982 None:intergenic
ATCTATAAAATTGATATGGA+AGG 0.532178 2_3:+30940498 None:intergenic
ACGCAGACCAGCTGAATGAA+GGG 0.538664 2_3:-30940720 Msa0287750:CDS
TGTTAGTTTCCACCTTTCAC+AGG 0.542839 2_3:-30940017 Msa0287750:three_prime_UTR
CCAGCTGAGCTAAGCTCACA+AGG 0.543579 2_3:-30940192 Msa0287750:three_prime_UTR
GTGGACCTTTGACCTTCTCA+CGG 0.544091 2_3:+30940857 None:intergenic
GTCGCTTGTTTCAACTTATG+CGG 0.544680 2_3:+30940811 None:intergenic
AAGGTCCGGGGTTCAAACCC+CGG 0.546686 2_3:+30940238 None:intergenic
CGCACACGATCTCTTCCGAT+TGG 0.547710 2_3:-30943054 Msa0287750:CDS
TGGAGAATCCCCTTCCTCAC+GGG 0.550577 2_3:+30942900 None:intergenic
TCCAATAGTAGGAGACCTAT+GGG 0.551867 2_3:-30940306 Msa0287750:three_prime_UTR
AAAGGGGTTCCCGTGAGGAA+GGG 0.562673 2_3:-30942909 Msa0287750:CDS
TTGTGGACTTGGTGAAGCAA+TGG 0.563332 2_3:-30942256 Msa0287750:CDS
GCATCTGGATGTAAGGCAGC+CGG 0.564627 2_3:-30940653 Msa0287750:CDS
TTTGAAGAATGTACTTGAGG+AGG 0.565288 2_3:-30942190 Msa0287750:CDS
GCGGTAACTGTTCTGTCGAT+AGG 0.571962 2_3:+30943125 None:intergenic
ACGCTACAACACCATGGCAG+AGG 0.576642 2_3:-30940744 Msa0287750:CDS
ATGTAAGGCAGCCGGATGCA+CGG 0.577781 2_3:-30940645 Msa0287750:CDS
TGCGAGAAGAGAGATACGAC+TGG 0.579475 2_3:+30943073 None:intergenic
CGCTACAACACCATGGCAGA+GGG 0.584181 2_3:-30940743 Msa0287750:CDS
ATGCAGGGCAGCTGGATACA+GGG 0.585703 2_3:-30940675 Msa0287750:CDS
GACAATGCATAAAATATGCA+AGG 0.589572 2_3:+30940219 None:intergenic
TTGGATCCAGGTGTTTCACT+AGG 0.589935 2_3:-30940589 Msa0287750:CDS
AATACTCAACATTGTTGTAG+AGG 0.592786 2_3:+30940156 None:intergenic
CTATCATTCTCGGTTCCTCA+GGG 0.594726 2_3:-30942231 Msa0287750:CDS
TCCTAAAGAAAACCGTGAGA+AGG 0.598029 2_3:-30940869 Msa0287750:CDS
GCAGCCGGATGCACGGCAGT+CGG 0.601262 2_3:-30940638 Msa0287750:CDS
GATCGAAGAAAAGAATGAGA+TGG 0.601328 2_3:+30943006 None:intergenic
ATAGGTCTCCTACTATTGGA+AGG 0.602195 2_3:+30940309 None:intergenic
ATGGAGAATCCCCTTCCTCA+CGG 0.613119 2_3:+30942899 None:intergenic
ATGCACGGCAGTCGGATACA+GGG 0.614645 2_3:-30940630 Msa0287750:CDS
GAAAACCGTGAGAAGGTCAA+AGG 0.614702 2_3:-30940862 Msa0287750:CDS
ATGAAGGGGAGTCAAATGCA+GGG 0.626276 2_3:-30940705 Msa0287750:CDS
ATGCAGGGGAGCAAGATGCA+GGG 0.636179 2_3:-30940690 Msa0287750:CDS
CGCAGACCAGCTGAATGAAG+GGG 0.641381 2_3:-30940719 Msa0287750:CDS
AAGGGTTCTGGTCAGAAAAG+GGG 0.652348 2_3:-30942925 Msa0287750:CDS
CATAAAATATGCAAGGTCCG+GGG 0.657923 2_3:+30940226 None:intergenic
GCGAGAAGAGAGATACGACT+GGG 0.658917 2_3:+30943074 None:intergenic
GTTGCGCAATCGGAGTAGGA+CGG 0.665262 2_3:+30943028 None:intergenic
TGAAGGGGAGTCAAATGCAG+GGG 0.669223 2_3:-30940704 Msa0287750:CDS
GCTTTACCTAGTGAAACACC+TGG 0.684772 2_3:+30940583 None:intergenic
GATGGAAATTTGACTCCCTG+AGG 0.694236 2_3:+30942216 None:intergenic
GCGCAATCGGAGTAGGACGG+TGG 0.704019 2_3:+30943031 None:intergenic
TCAGAAAAGGGGTTCCCGTG+AGG 0.706408 2_3:-30942914 Msa0287750:CDS
GTGATGACGCTACAACACCA+TGG 0.716250 2_3:-30940750 Msa0287750:CDS
GTTGTAGCGTCATCACTGAG+AGG 0.716312 2_3:+30940757 None:intergenic
GTGGACTTGGTGAAGCAATG+GGG 0.770343 2_3:-30942254 Msa0287750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGTAATTAACAATTA+AGG + chr2_3:30940270-30940289 None:intergenic 10.0%
!! TTAAATCTATAAAATTGATA+TGG + chr2_3:30942628-30942647 None:intergenic 10.0%
!!! ATATCAATTTTATAGATTTA+AGG - chr2_3:30942627-30942646 Msa0287750:intron 10.0%
!!! TAAATTTTTTATTTTGAAAG+AGG + chr2_3:30942771-30942790 None:intergenic 10.0%
!!! ATTTTTAAGAATGAAATGTT+TGG + chr2_3:30941097-30941116 None:intergenic 15.0%
!!! CACTTTAGTATATTTTATTA+CGG + chr2_3:30941183-30941202 None:intergenic 15.0%
!! AATAAAATATACTAAAGTGA+CGG - chr2_3:30941184-30941203 Msa0287750:intron 15.0%
!!! TAACAAATGTATTTTTGTTT+TGG - chr2_3:30942079-30942098 Msa0287750:intron 15.0%
!! AGACTTACAAAAATTGAAAA+TGG + chr2_3:30940242-30940261 None:intergenic 20.0%
!! AATTGAAAAACACAATCAAA+TGG + chr2_3:30940335-30940354 None:intergenic 20.0%
!! CTTTACGAATAGATTAAATA+TGG - chr2_3:30940496-30940515 Msa0287750:three_prime_UTR 20.0%
!!! TAAGAATAATATTGGTTGTA+AGG - chr2_3:30940953-30940972 Msa0287750:intron 20.0%
!! TCTATAGAAATGTTAGAAAT+TGG - chr2_3:30941033-30941052 Msa0287750:intron 20.0%
!!! CTTTTTTACAAAAAATTGAG+AGG + chr2_3:30941157-30941176 None:intergenic 20.0%
!!! ACAACTTTAATTTAGTTTCA+GGG - chr2_3:30941407-30941426 Msa0287750:intron 20.0%
!! AAATTTATAGGATCATCTAA+TGG + chr2_3:30941775-30941794 None:intergenic 20.0%
!! ATTAGATGATCCTATAAATT+TGG - chr2_3:30941774-30941793 Msa0287750:intron 20.0%
!! ATCATTTACTCCAAATTTAT+AGG + chr2_3:30941787-30941806 None:intergenic 20.0%
!!! TCTTTTACATATTGAATTTG+AGG - chr2_3:30942031-30942050 Msa0287750:intron 20.0%
!!! AAATGTATTTTTGTTTTGGT+TGG - chr2_3:30942083-30942102 Msa0287750:intron 20.0%
!! TTAGAACATTAATTAGTGTA+TGG + chr2_3:30942142-30942161 None:intergenic 20.0%
!!! CTATATTCTCTTATGTTTTT+TGG - chr2_3:30942215-30942234 Msa0287750:CDS 20.0%
!! TTAAGATTATGATCATGATT+AGG + chr2_3:30942563-30942582 None:intergenic 20.0%
!!! AACTCTTAAGATTATTTTCA+AGG - chr2_3:30942581-30942600 Msa0287750:intron 20.0%
!!! ACTCTTAAGATTATTTTCAA+GGG - chr2_3:30942582-30942601 Msa0287750:intron 20.0%
!! ATCTATAAAATTGATATGGA+AGG + chr2_3:30942624-30942643 None:intergenic 20.0%
!!! AATTTTATAGATTTAAGGCT+GGG - chr2_3:30942632-30942651 Msa0287750:intron 20.0%
!!! TTTTTTATTTTGAAAGAGGA+AGG + chr2_3:30942767-30942786 None:intergenic 20.0%
!!! GTGCAGTTAATTATTTTTTT+GGG + chr2_3:30942845-30942864 None:intergenic 20.0%
!!! GCATATAATTTTCAATTTTG+CGG - chr2_3:30943042-30943061 Msa0287750:CDS 20.0%
! ATTCCATACATGTATATTTG+TGG - chr2_3:30940292-30940311 Msa0287750:three_prime_UTR 25.0%
! TAACCACAAATATACATGTA+TGG + chr2_3:30940298-30940317 None:intergenic 25.0%
! ACATGTATATTTGTGGTTAT+TGG - chr2_3:30940299-30940318 Msa0287750:three_prime_UTR 25.0%
! CAAATTATTCAATGTGATTG+TGG - chr2_3:30940381-30940400 Msa0287750:three_prime_UTR 25.0%
!!! TCAAGTACTGATTGAATTTT+GGG - chr2_3:30940564-30940583 Msa0287750:CDS 25.0%
!!! AAGTTGGATTTTTTTTGATC+TGG - chr2_3:30940623-30940642 Msa0287750:CDS 25.0%
!!! AGTTGGATTTTTTTTGATCT+GGG - chr2_3:30940624-30940643 Msa0287750:CDS 25.0%
! AGATTAGGACACATTATATT+AGG - chr2_3:30940653-30940672 Msa0287750:CDS 25.0%
! GAATATTGATAAGACTTTGA+AGG - chr2_3:30940687-30940706 Msa0287750:CDS 25.0%
!!! GGTTAGTTTGATAATTATCA+TGG - chr2_3:30940750-30940769 Msa0287750:CDS 25.0%
!!! GATAATTATCATGGTTTTAC+TGG - chr2_3:30940759-30940778 Msa0287750:CDS 25.0%
!!! TACTTCACTGATTTTGATTT+TGG - chr2_3:30940824-30940843 Msa0287750:CDS 25.0%
!!! GATTTTGATTTTGGTCTTTT+AGG - chr2_3:30940833-30940852 Msa0287750:CDS 25.0%
!! TCTATAGAAGCTTTAATCAA+TGG + chr2_3:30941021-30941040 None:intergenic 25.0%
! ATTCACAATTAAAGCATACA+TGG - chr2_3:30941229-30941248 Msa0287750:intron 25.0%
! CTGAAACTAAATTAAAGTTG+TGG + chr2_3:30941408-30941427 None:intergenic 25.0%
!! CACAACTTTAATTTAGTTTC+AGG - chr2_3:30941406-30941425 Msa0287750:intron 25.0%
! TAATACACCAAACATAGTAA+GGG + chr2_3:30941557-30941576 None:intergenic 25.0%
! ATAATACACCAAACATAGTA+AGG + chr2_3:30941558-30941577 None:intergenic 25.0%
! TTGGAGTAAATGATTCAATT+CGG - chr2_3:30941793-30941812 Msa0287750:intron 25.0%
! ATTAGCACTATAAGATCAAT+TGG + chr2_3:30941949-30941968 None:intergenic 25.0%
!!! CAATTTTATAGATTTAAGGC+TGG - chr2_3:30942631-30942650 Msa0287750:intron 25.0%
! TTCTTATGTAAGGAATAGTT+GGG + chr2_3:30942743-30942762 None:intergenic 25.0%
! ATTCTTATGTAAGGAATAGT+TGG + chr2_3:30942744-30942763 None:intergenic 25.0%
!!! GGTGCAGTTAATTATTTTTT+TGG + chr2_3:30942846-30942865 None:intergenic 25.0%
!! ATAATTTTCAATTTTGCGGA+TGG - chr2_3:30943046-30943065 Msa0287750:CDS 25.0%
!! TTTTCAATTGACTCTGATTG+TGG - chr2_3:30940346-30940365 Msa0287750:three_prime_UTR 30.0%
!!! GTCAAGTACTGATTGAATTT+TGG - chr2_3:30940563-30940582 Msa0287750:CDS 30.0%
!!! CAAGTACTGATTGAATTTTG+GGG - chr2_3:30940565-30940584 Msa0287750:CDS 30.0%
!! GATTTGTGGTTTTTGAAAGT+TGG - chr2_3:30940607-30940626 Msa0287750:CDS 30.0%
TCACTTTGATCAATATTGTC+AGG - chr2_3:30940729-30940748 Msa0287750:CDS 30.0%
AGTACATTCTTCAAAATGGA+TGG + chr2_3:30940924-30940943 None:intergenic 30.0%
CTCAAGTACATTCTTCAAAA+TGG + chr2_3:30940928-30940947 None:intergenic 30.0%
!! CATTTTGAAGAATGTACTTG+AGG - chr2_3:30940926-30940945 Msa0287750:intron 30.0%
GAGGAGGTTAAGAATAATAT+TGG - chr2_3:30940945-30940964 Msa0287750:intron 30.0%
TCATGGCAATCTTGAAAAAT+GGG - chr2_3:30940983-30941002 Msa0287750:intron 30.0%
GAAAGAGTGATTTCTTTGAA+TGG - chr2_3:30941131-30941150 Msa0287750:intron 30.0%
TATGAACACAGTATGAAGTT+AGG - chr2_3:30941266-30941285 Msa0287750:intron 30.0%
TGTTTATTCTAAATGCAGAG+TGG - chr2_3:30941293-30941312 Msa0287750:intron 30.0%
GTTTATTCTAAATGCAGAGT+GGG - chr2_3:30941294-30941313 Msa0287750:intron 30.0%
AAATGAGTGAACAACAGAAA+GGG + chr2_3:30941323-30941342 None:intergenic 30.0%
! GAACTTCTAATCAAGAGTAT+TGG - chr2_3:30941481-30941500 Msa0287750:intron 30.0%
AAAATGACCCTTACTATGTT+TGG - chr2_3:30941547-30941566 Msa0287750:intron 30.0%
!! TTGTAACTAGCTTTCAATTC+AGG - chr2_3:30941602-30941621 Msa0287750:intron 30.0%
ATGAATGATTGAAGTCCTTA+AGG - chr2_3:30942106-30942125 Msa0287750:intron 30.0%
!! TATGCGAACTTTAAAACAGA+CGG + chr2_3:30942169-30942188 None:intergenic 30.0%
!!! TATGTTTTTTGGACAGATCA+TGG - chr2_3:30942226-30942245 Msa0287750:CDS 30.0%
!! TAAGATTATTTTCAAGGGCA+AGG - chr2_3:30942587-30942606 Msa0287750:intron 30.0%
!! AAGATTATTTTCAAGGGCAA+GGG - chr2_3:30942588-30942607 Msa0287750:intron 30.0%
! TAATTAACTGCACCTCTTTT+TGG - chr2_3:30942852-30942871 Msa0287750:intron 30.0%
GACAATGCATAAAATATGCA+AGG + chr2_3:30942903-30942922 None:intergenic 30.0%
AATACTCAACATTGTTGTAG+AGG + chr2_3:30942966-30942985 None:intergenic 30.0%
! GTTGAGTATTGTCAACTATT+TGG - chr2_3:30942976-30942995 Msa0287750:CDS 30.0%
TGTTGCAATTACTATCTTAG+TGG - chr2_3:30943020-30943039 Msa0287750:CDS 30.0%
TTATTCAACATTCCTGTGAA+AGG + chr2_3:30943117-30943136 None:intergenic 30.0%
GTTGAATAAATTGTGCATGT+TGG - chr2_3:30943128-30943147 Msa0287750:CDS 30.0%
GATCGAAGAAAAGAATGAGA+TGG + chr2_3:30940116-30940135 None:intergenic 35.0%
CTATTCGATTTCAGGATTCT+CGG - chr2_3:30940167-30940186 Msa0287750:three_prime_UTR 35.0%
TATTCGATTTCAGGATTCTC+GGG - chr2_3:30940168-30940187 Msa0287750:three_prime_UTR 35.0%
!! GAATTGACTTGTGTTTGACT+TGG - chr2_3:30940541-30940560 Msa0287750:exon 35.0%
!! ATTGAATTTTGGGGATCTGA+AGG - chr2_3:30940574-30940593 Msa0287750:CDS 35.0%
!! TTGAATTTTGGGGATCTGAA+GGG - chr2_3:30940575-30940594 Msa0287750:CDS 35.0%
!! GTCTTTTAGGAACCTTATTG+TGG - chr2_3:30940846-30940865 Msa0287750:CDS 35.0%
TTTGAAGAATGTACTTGAGG+AGG - chr2_3:30940929-30940948 Msa0287750:intron 35.0%
!!! CATTTTTCAAGATTGCCATG+AGG + chr2_3:30940984-30941003 None:intergenic 35.0%
CTCATGGCAATCTTGAAAAA+TGG - chr2_3:30940982-30941001 Msa0287750:intron 35.0%
CAAATGAGTGAACAACAGAA+AGG + chr2_3:30941324-30941343 None:intergenic 35.0%
! TTGCTGAATTTTGTTCTCAC+AGG - chr2_3:30941437-30941456 Msa0287750:intron 35.0%
AGTCATAAACTGTTTGTGCA+TGG - chr2_3:30941512-30941531 Msa0287750:intron 35.0%
!! AACTAGCTTTCAATTCAGGT+AGG - chr2_3:30941606-30941625 Msa0287750:intron 35.0%
GCAAATGCACATGCAAATAT+AGG - chr2_3:30941644-30941663 Msa0287750:intron 35.0%
GTCATCACTATGTGATTTCA+TGG + chr2_3:30941826-30941845 None:intergenic 35.0%
TATGGTGTACATGTTCCTTA+AGG + chr2_3:30942124-30942143 None:intergenic 35.0%
!!! ATCATCCACTTTTTCATCTG+TGG + chr2_3:30942284-30942303 None:intergenic 35.0%
AGAGGAAGGATTCTTATGTA+AGG + chr2_3:30942753-30942772 None:intergenic 35.0%
ATTGGAAGGATTCTTGTCTA+GGG + chr2_3:30942799-30942818 None:intergenic 35.0%
TATTGGAAGGATTCTTGTCT+AGG + chr2_3:30942800-30942819 None:intergenic 35.0%
ACAAGAATCCTTCCAATAGT+AGG - chr2_3:30942802-30942821 Msa0287750:intron 35.0%
!!! TTTTTTGGGTGTATACCCAT+AGG + chr2_3:30942831-30942850 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+CGG + chr2_3:30942898-30942917 None:intergenic 35.0%
CTCTCTCTCTATTCGATTTC+AGG - chr2_3:30940159-30940178 Msa0287750:three_prime_UTR 40.0%
! AAAGGGTTCTGGTCAGAAAA+GGG - chr2_3:30940193-30940212 Msa0287750:three_prime_UTR 40.0%
!!! AAGGGTGTTTTGTGGATTTG+TGG - chr2_3:30940593-30940612 Msa0287750:CDS 40.0%
TAGGAACCTTATTGTGGACT+TGG - chr2_3:30940852-30940871 Msa0287750:CDS 40.0%
TCTTGAAAAATGGGATGTGC+AGG - chr2_3:30940992-30941011 Msa0287750:intron 40.0%
!! AGTGACGGATTTCAGATTTG+TGG - chr2_3:30941199-30941218 Msa0287750:intron 40.0%
TGTTCACTCATTTGTGCAGA+TGG - chr2_3:30941330-30941349 Msa0287750:intron 40.0%
CAAGACGATCATAGAGAAGA+AGG - chr2_3:30941694-30941713 Msa0287750:intron 40.0%
ACGATCATAGAGAAGAAGGA+AGG - chr2_3:30941698-30941717 Msa0287750:intron 40.0%
CGATCATAGAGAAGAAGGAA+GGG - chr2_3:30941699-30941718 Msa0287750:intron 40.0%
GATCATAGAGAAGAAGGAAG+GGG - chr2_3:30941700-30941719 Msa0287750:intron 40.0%
AGTATGCGCAGGAGAAAATT+CGG - chr2_3:30941744-30941763 Msa0287750:intron 40.0%
CTGAAGCATAAGAGCAAGTA+TGG + chr2_3:30941979-30941998 None:intergenic 40.0%
AGATGAGATGAAATCGACGT+AGG - chr2_3:30942055-30942074 Msa0287750:intron 40.0%
TCCTAAAGAAAACCGTGAGA+AGG - chr2_3:30942250-30942269 Msa0287750:CDS 40.0%
! ACCTTCTCACGGTTTTCTTT+AGG + chr2_3:30942254-30942273 None:intergenic 40.0%
AAGGTCCACAGATGAAAAAG+TGG - chr2_3:30942276-30942295 Msa0287750:intron 40.0%
GTCGCTTGTTTCAACTTATG+CGG + chr2_3:30942311-30942330 None:intergenic 40.0%
CAAGCGACTAAGAATCTACA+TGG - chr2_3:30942323-30942342 Msa0287750:intron 40.0%
! TCTGTCCTTTTTCTTGTACG+AGG - chr2_3:30942702-30942721 Msa0287750:intron 40.0%
ATAGGTCTCCTACTATTGGA+AGG + chr2_3:30942813-30942832 None:intergenic 40.0%
TTCCAATAGTAGGAGACCTA+TGG - chr2_3:30942812-30942831 Msa0287750:intron 40.0%
TCCAATAGTAGGAGACCTAT+GGG - chr2_3:30942813-30942832 Msa0287750:intron 40.0%
CATAAAATATGCAAGGTCCG+GGG + chr2_3:30942896-30942915 None:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG + chr2_3:30942897-30942916 None:intergenic 40.0%
! TGTTAGTTTCCACCTTTCAC+AGG - chr2_3:30943102-30943121 Msa0287750:CDS 40.0%
TTCAACATTCCTGTGAAAGG+TGG + chr2_3:30943114-30943133 None:intergenic 40.0%
TTCACGAAAGAGAGAGAGAG+AGG + chr2_3:30940021-30940040 None:intergenic 45.0%
GAATGAGATGGTTGCGCAAT+CGG + chr2_3:30940104-30940123 None:intergenic 45.0%
ATAGAGAGAGAGAGAGAGAG+AGG + chr2_3:30940151-30940170 None:intergenic 45.0%
TTTCAGGATTCTCGGGAGAA+AGG - chr2_3:30940175-30940194 Msa0287750:three_prime_UTR 45.0%
TTCAGGATTCTCGGGAGAAA+GGG - chr2_3:30940176-30940195 Msa0287750:three_prime_UTR 45.0%
! GAAAGGGTTCTGGTCAGAAA+AGG - chr2_3:30940192-30940211 Msa0287750:three_prime_UTR 45.0%
! AAGGGTTCTGGTCAGAAAAG+GGG - chr2_3:30940194-30940213 Msa0287750:three_prime_UTR 45.0%
!! ACTCTGATTGTGGCTTGTTG+TGG - chr2_3:30940356-30940375 Msa0287750:three_prime_UTR 45.0%
! TGATCTGGGTGTTGCAGATT+AGG - chr2_3:30940638-30940657 Msa0287750:CDS 45.0%
GCTTCACCAAGTCCACAATA+AGG + chr2_3:30940861-30940880 None:intergenic 45.0%
!! TTGTGGACTTGGTGAAGCAA+TGG - chr2_3:30940863-30940882 Msa0287750:CDS 45.0%
!! TGTGGACTTGGTGAAGCAAT+GGG - chr2_3:30940864-30940883 Msa0287750:CDS 45.0%
AGCAATGGGGCTATCATTCT+CGG - chr2_3:30940878-30940897 Msa0287750:CDS 45.0%
CTATCATTCTCGGTTCCTCA+GGG - chr2_3:30940888-30940907 Msa0287750:intron 45.0%
GATGGAAATTTGACTCCCTG+AGG + chr2_3:30940906-30940925 None:intergenic 45.0%
GGTTGTAAGGTTCTACCTCA+TGG - chr2_3:30940966-30940985 Msa0287750:intron 45.0%
GAAAACCGTGAGAAGGTCAA+AGG - chr2_3:30942257-30942276 Msa0287750:CDS 45.0%
AATGAAGGGGAGTCAAATGC+AGG - chr2_3:30942413-30942432 Msa0287750:CDS 45.0%
ATGAAGGGGAGTCAAATGCA+GGG - chr2_3:30942414-30942433 Msa0287750:CDS 45.0%
! ATACAGGGCATCTGGATGTA+AGG - chr2_3:30942459-30942478 Msa0287750:CDS 45.0%
TTGGATCCAGGTGTTTCACT+AGG - chr2_3:30942530-30942549 Msa0287750:intron 45.0%
GCTTTACCTAGTGAAACACC+TGG + chr2_3:30942539-30942558 None:intergenic 45.0%
ACCCATAGGTCTCCTACTAT+TGG + chr2_3:30942817-30942836 None:intergenic 45.0%
!! ACTGCACCTCTTTTTGGTAG+TGG - chr2_3:30942858-30942877 Msa0287750:intron 45.0%
GCGGTAACTGTTCTGTCGAT+AGG + chr2_3:30939997-30940016 None:intergenic 50.0%
GAAAGAGAGAGAGAGAGGAG+CGG + chr2_3:30940016-30940035 None:intergenic 50.0%
GCGAGAAGAGAGATACGACT+GGG + chr2_3:30940048-30940067 None:intergenic 50.0%
TGCGAGAAGAGAGATACGAC+TGG + chr2_3:30940049-30940068 None:intergenic 50.0%
GAGAGAGAGAGAGAGGAAGT+CGG + chr2_3:30940144-30940163 None:intergenic 50.0%
ATTCTCGGGAGAAAGGGTTC+TGG - chr2_3:30940182-30940201 Msa0287750:three_prime_UTR 50.0%
ATGGAGAATCCCCTTCCTCA+CGG + chr2_3:30940223-30940242 None:intergenic 50.0%
!!! GGGATCTGAAGGGTGTTTTG+TGG - chr2_3:30940585-30940604 Msa0287750:CDS 50.0%
!! GTGGACTTGGTGAAGCAATG+GGG - chr2_3:30940865-30940884 Msa0287750:CDS 50.0%
GCTATCATTCTCGGTTCCTC+AGG - chr2_3:30940887-30940906 Msa0287750:intron 50.0%
! AAGGGGTCGTGTTTCTGATG+TGG - chr2_3:30941717-30941736 Msa0287750:intron 50.0%
! AGGGGTCGTGTTTCTGATGT+GGG - chr2_3:30941718-30941737 Msa0287750:intron 50.0%
GATGTGGGAATAGTATGCGC+AGG - chr2_3:30941733-30941752 Msa0287750:intron 50.0%
GTGGACCTTTGACCTTCTCA+CGG + chr2_3:30942265-30942284 None:intergenic 50.0%
GTTGTAGCGTCATCACTGAG+AGG + chr2_3:30942365-30942384 None:intergenic 50.0%
! GTGATGACGCTACAACACCA+TGG - chr2_3:30942369-30942388 Msa0287750:intron 50.0%
ACGCAGACCAGCTGAATGAA+GGG - chr2_3:30942399-30942418 Msa0287750:CDS 50.0%
TTGACTCCCCTTCATTCAGC+TGG + chr2_3:30942409-30942428 None:intergenic 50.0%
TGAAGGGGAGTCAAATGCAG+GGG - chr2_3:30942415-30942434 Msa0287750:CDS 50.0%
GCATCTAGATGCAGTGCAGT+TGG - chr2_3:30942511-30942530 Msa0287750:intron 50.0%
GAGCGCCTCGTACAAGAAAA+AGG + chr2_3:30942710-30942729 None:intergenic 50.0%
!!! CACCTCTTTTTGGTAGTGGC+CGG - chr2_3:30942862-30942881 Msa0287750:intron 50.0%
!!! ACCTCTTTTTGGTAGTGGCC+GGG - chr2_3:30942863-30942882 Msa0287750:intron 50.0%
TGAGCTTAGCTCAGCTGGTT+GGG + chr2_3:30942925-30942944 None:intergenic 50.0%
CGCACACGATCTCTTCCGAT+TGG - chr2_3:30940065-30940084 Msa0287750:three_prime_UTR 55.0%
GTTGCGCAATCGGAGTAGGA+CGG + chr2_3:30940094-30940113 None:intergenic 55.0%
GATGGTTGCGCAATCGGAGT+AGG + chr2_3:30940098-30940117 None:intergenic 55.0%
GAGAGAGAGAGGAAGTCGGT+GGG + chr2_3:30940140-30940159 None:intergenic 55.0%
AGAGAGAGAGAGGAAGTCGG+TGG + chr2_3:30940141-30940160 None:intergenic 55.0%
TCAGAAAAGGGGTTCCCGTG+AGG - chr2_3:30940205-30940224 Msa0287750:three_prime_UTR 55.0%
AAAAGGGGTTCCCGTGAGGA+AGG - chr2_3:30940209-30940228 Msa0287750:three_prime_UTR 55.0%
AAAGGGGTTCCCGTGAGGAA+GGG - chr2_3:30940210-30940229 Msa0287750:three_prime_UTR 55.0%
TGGAGAATCCCCTTCCTCAC+GGG + chr2_3:30940222-30940241 None:intergenic 55.0%
ACGCTACAACACCATGGCAG+AGG - chr2_3:30942375-30942394 Msa0287750:intron 55.0%
CGCTACAACACCATGGCAGA+GGG - chr2_3:30942376-30942395 Msa0287750:intron 55.0%
GACGCAGACCAGCTGAATGA+AGG - chr2_3:30942398-30942417 Msa0287750:CDS 55.0%
CGCAGACCAGCTGAATGAAG+GGG - chr2_3:30942400-30942419 Msa0287750:CDS 55.0%
AATGCAGGGGAGCAAGATGC+AGG - chr2_3:30942428-30942447 Msa0287750:CDS 55.0%
ATGCAGGGGAGCAAGATGCA+GGG - chr2_3:30942429-30942448 Msa0287750:CDS 55.0%
ATGCAGGGCAGCTGGATACA+GGG - chr2_3:30942444-30942463 Msa0287750:CDS 55.0%
GCATCTGGATGTAAGGCAGC+CGG - chr2_3:30942466-30942485 Msa0287750:CDS 55.0%
ATGTAAGGCAGCCGGATGCA+CGG - chr2_3:30942474-30942493 Msa0287750:intron 55.0%
ATGCACGGCAGTCGGATACA+GGG - chr2_3:30942489-30942508 Msa0287750:intron 55.0%
GATGCAGTGCAGTTGGATCC+AGG - chr2_3:30942518-30942537 Msa0287750:intron 55.0%
!!! CCTCTTTTTGGTAGTGGCCG+GGG - chr2_3:30942864-30942883 Msa0287750:intron 55.0%
GTGAGCTTAGCTCAGCTGGT+TGG + chr2_3:30942926-30942945 None:intergenic 55.0%
CCTTGTGAGCTTAGCTCAGC+TGG + chr2_3:30942930-30942949 None:intergenic 55.0%
CCAGCTGAGCTAAGCTCACA+AGG - chr2_3:30942927-30942946 Msa0287750:CDS 55.0%
GGAGTAGGACGGTGGCCAAT+CGG + chr2_3:30940083-30940102 None:intergenic 60.0%
AAGGGGTTCCCGTGAGGAAG+GGG - chr2_3:30940211-30940230 Msa0287750:three_prime_UTR 60.0%
GATGCAGGGCAGCTGGATAC+AGG - chr2_3:30942443-30942462 Msa0287750:CDS 60.0%
! GCAGCTGGATACAGGGCATC+TGG - chr2_3:30942451-30942470 Msa0287750:CDS 60.0%
GTATCCGACTGCCGTGCATC+CGG + chr2_3:30942488-30942507 None:intergenic 60.0%
GATGCACGGCAGTCGGATAC+AGG - chr2_3:30942488-30942507 Msa0287750:intron 60.0%
CCCCGGCCACTACCAAAAAG+AGG + chr2_3:30942867-30942886 None:intergenic 60.0%
AAGGTCCGGGGTTCAAACCC+CGG + chr2_3:30942884-30942903 None:intergenic 60.0%
GCGCAATCGGAGTAGGACGG+TGG + chr2_3:30940091-30940110 None:intergenic 65.0%
TGGTCTGCGTCCCTCTGCCA+TGG + chr2_3:30942389-30942408 None:intergenic 65.0%
GGAGCAAGATGCAGGGCAGC+TGG - chr2_3:30942436-30942455 Msa0287750:CDS 65.0%
AGTGGCCGGGGTTTGAACCC+CGG - chr2_3:30942876-30942895 Msa0287750:intron 65.0%
GCAGCCGGATGCACGGCAGT+CGG - chr2_3:30942481-30942500 Msa0287750:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 30939990 30943151 30939990 ID=Msa0287750;Name=Msa0287750
chr2_3 mRNA 30939990 30943151 30939990 ID=Msa0287750-mRNA-1;Parent=Msa0287750;Name=Msa0287750-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0.66|0.75|1|1|1|4|562|288
chr2_3 exon 30939990 30940899 30939990 ID=Msa0287750-mRNA-1:exon:10591;Parent=Msa0287750-mRNA-1
chr2_3 exon 30942128 30942286 30942128 ID=Msa0287750-mRNA-1:exon:10590;Parent=Msa0287750-mRNA-1
chr2_3 exon 30942391 30942486 30942391 ID=Msa0287750-mRNA-1:exon:10589;Parent=Msa0287750-mRNA-1
chr2_3 exon 30942888 30943151 30942888 ID=Msa0287750-mRNA-1:exon:10588;Parent=Msa0287750-mRNA-1
chr2_3 CDS 30942888 30943151 30942888 ID=Msa0287750-mRNA-1:cds;Parent=Msa0287750-mRNA-1
chr2_3 CDS 30942391 30942486 30942391 ID=Msa0287750-mRNA-1:cds;Parent=Msa0287750-mRNA-1
chr2_3 CDS 30942128 30942286 30942128 ID=Msa0287750-mRNA-1:cds;Parent=Msa0287750-mRNA-1
chr2_3 CDS 30940552 30940899 30940552 ID=Msa0287750-mRNA-1:cds;Parent=Msa0287750-mRNA-1
chr2_3 three_prime_UTR 30939990 30940551 30939990 ID=Msa0287750-mRNA-1:three_prime_utr;Parent=Msa0287750-mRNA-1
Gene Sequence

>Msa0287750

AACTCCTATCGACAGAACAGTTACCGCTCCTCTCTCTCTCTCTTTCGTGAACTCTCCCAGTCGTATCTCTCTTCTCGCACACGATCTCTTCCGATTGGCCACCGTCCTACTCCGATTGCGCAACCATCTCATTCTTTTCTTCGATCACCCACCGACTTCCTCTCTCTCTCTCTCTCTCTATTCGATTTCAGGATTCTCGGGAGAAAGGGTTCTGGTCAGAAAAGGGGTTCCCGTGAGGAAGGGGATTCTCCATTTTCAATTTTTATTAGGACACATTATATTAGGAAAGCTCATGAGAATATTGATAAGACTTTGAAGGCTTCTGAGATTATATTGTCTCACTTTGATCAATATTGTCAGGAACCTTATTGTGGACTTGGTGAAGCAATGGGGCTATCATTCTCGGTTCCTCAGGGAGTCAAATTTCCATCCATTTTGAAGAATGTACTTGAGGAGGTTAAGAATAATATTGGTTGTAAGGTTCTACCTCATGGCAATCTTGAAAAATGGGATGTGCAGATCATGGCTCCTAAAGAAAACCGTGAGAAGGTCAAAGGTCCACAGATGAAAAAGTGGATGATTATGCCGCATAAGTTGAAACAAGCGACTAAGAATCTACATGGATTAGCTTTGAAGTCGCCTCTCAGTGATGACGCTACAACACCATGGCAGAGGGACGCAGACCAGCTGAATGAAGGGGAGTCAAATGCAGGGGAGCAAGATGCAGGGCAGCTGGATACAGGGCATCTGGATGTAAGGCAGCCGGATGCACGGCAGTCGGATACAGGGCATCTAGATGCAGTGCAGTTGGATCCAGGTGTTTCACTAGGTAAAGCACCTAATCATGATCATAATCTTAACTCTTAA
Protein sequence

>Msa0287750

NSYRQNSYRSSLSLFRELSQSYLSSRTRSLPIGHRPTPIAQPSHSFLRSPTDFLSLSLSLFDFRILGRKGSGQKRGSREEGDSPFSIFIRTHYIRKAHENIDKTLKASEIILSHFDQYCQEPYCGLGEAMGLSFSVPQGVKFPSILKNVLEEVKNNIGCKVLPHGNLEKWDVQIMAPKENREKVKGPQMKKWMIMPHKLKQATKNLHGLALKSPLSDDATTPWQRDADQLNEGESNAGEQDAGQLDTGHLDVRQPDARQSDTGHLDAVQLDPGVSLGKAPNHDHNLNS*