AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1450080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450080 MtrunA17_Chr4g0037741 75.926 54 13 0 121 174 74 127 4.17e-21 89.7
Msa1450080 MtrunA17_Chr4g0037761 68.519 54 17 0 121 174 74 127 1.57e-18 82.4
Msa1450080 MtrunA17_Chr4g0037791 62.963 54 20 0 121 174 144 197 1.76e-16 78.2
Msa1450080 MtrunA17_Chr3g0125141 52.381 63 30 0 176 238 1 63 6.52e-14 65.9
Msa1450080 MtrunA17_Chr1g0197101 90.625 32 3 0 90 121 55 86 5.15e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450080 AT3G18630.1 57.407 54 23 0 121 174 172 225 2.86e-14 72.4

Find 110 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTCTCTATTCGATTTC+AGG 0.110623 tig0032786:+4321 Msa1450080:CDS
CTCAAGTACATTCTTCAAAA+TGG 0.251262 tig0032786:-5087 Msa1450080:intergenic
GTTGAGTATTGTCAACTATT+TGG 0.270362 tig0032786:+7138 Msa1450080:three_prime_UTR
AGATTAGGACACATTATATT+AGG 0.275665 tig0032786:+4815 Msa1450080:intron
GAGGAGGTTAAGAATAATAT+TGG 0.300498 tig0032786:+5107 Msa1450080:CDS
TAAGAATAATATTGGTTGTA+AGG 0.355976 tig0032786:+5115 Msa1450080:CDS
CCTTGTGAGCTTAGCTCAGC+TGG 0.366709 tig0032786:-7089 Msa1450080:intergenic
TTAAATCTATAAAATTGATA+TGG 0.366835 tig0032786:-6787 Msa1450080:intergenic
GATGCAGGGCAGCTGGATAC+AGG 0.367904 tig0032786:+6605 Msa1450080:CDS
GAGCGCCTCGTACAAGAAAA+AGG 0.373216 tig0032786:-6869 Msa1450080:intergenic
TTGACTCCCCTTCATTCAGC+TGG 0.374906 tig0032786:-6568 Msa1450080:intergenic
GATGCACGGCAGTCGGATAC+AGG 0.375011 tig0032786:+6650 Msa1450080:CDS
GCTTCACCAAGTCCACAATA+AGG 0.376161 tig0032786:-5020 Msa1450080:intergenic
TGAGCTTAGCTCAGCTGGTT+GGG 0.376711 tig0032786:-7084 Msa1450080:intergenic
CTATTCGATTTCAGGATTCT+CGG 0.377459 tig0032786:+4329 Msa1450080:CDS
GAATGAGATGGTTGCGCAAT+CGG 0.381997 tig0032786:-4259 Msa1450080:intergenic
ATTCTTATGTAAGGAATAGT+TGG 0.392168 tig0032786:-6903 Msa1450080:intergenic
TGTTGCAATTACTATCTTAG+TGG 0.392904 tig0032786:+7182 Msa1450080:three_prime_UTR
GCTATCATTCTCGGTTCCTC+AGG 0.399999 tig0032786:+5049 Msa1450080:CDS
TGCATAAAATATGCAAGGTC+CGG 0.400962 tig0032786:-7057 Msa1450080:intergenic
TTAAGATTATGATCATGATT+AGG 0.410727 tig0032786:-6722 Msa1450080:intergenic
TAGGAACCTTATTGTGGACT+TGG 0.414406 tig0032786:+5014 Msa1450080:intron
ACCCATAGGTCTCCTACTAT+TGG 0.414819 tig0032786:-6976 Msa1450080:intergenic
GCAGCTGGATACAGGGCATC+TGG 0.424953 tig0032786:+6613 Msa1450080:CDS
TATTCGATTTCAGGATTCTC+GGG 0.426645 tig0032786:+4330 Msa1450080:CDS
TATTGGAAGGATTCTTGTCT+AGG 0.432089 tig0032786:-6959 Msa1450080:intergenic
GAATATTGATAAGACTTTGA+AGG 0.433586 tig0032786:+4849 Msa1450080:CDS
TTGAATAAATTGTGCATGTT+GGG 0.441819 tig0032786:+7291 Msa1450080:three_prime_UTR
TCACTTTGATCAATATTGTC+AGG 0.442772 tig0032786:+4891 Msa1450080:CDS
TTTCAGGATTCTCGGGAGAA+AGG 0.450201 tig0032786:+4337 Msa1450080:CDS
TTCAGGATTCTCGGGAGAAA+GGG 0.455367 tig0032786:+4338 Msa1450080:CDS
ACAAGAATCCTTCCAATAGT+AGG 0.455811 tig0032786:+6964 Msa1450080:three_prime_UTR
GGAGCAAGATGCAGGGCAGC+TGG 0.459296 tig0032786:+6598 Msa1450080:CDS
GAAAGGGTTCTGGTCAGAAA+AGG 0.459541 tig0032786:+4354 Msa1450080:CDS
GTGAGCTTAGCTCAGCTGGT+TGG 0.459734 tig0032786:-7085 Msa1450080:intergenic
TTCCAATAGTAGGAGACCTA+TGG 0.459868 tig0032786:+6974 Msa1450080:three_prime_UTR
AAGGGGTTCCCGTGAGGAAG+GGG 0.470151 tig0032786:+4373 Msa1450080:CDS
GTTGAATAAATTGTGCATGT+TGG 0.475595 tig0032786:+7290 Msa1450080:three_prime_UTR
AGCAATGGGGCTATCATTCT+CGG 0.477510 tig0032786:+5040 Msa1450080:CDS
AATGCAGGGGAGCAAGATGC+AGG 0.477678 tig0032786:+6590 Msa1450080:CDS
ATTCTCGGGAGAAAGGGTTC+TGG 0.479737 tig0032786:+4344 Msa1450080:CDS
TTCTTATGTAAGGAATAGTT+GGG 0.482757 tig0032786:-6902 Msa1450080:intergenic
AAAGGGTTCTGGTCAGAAAA+GGG 0.485467 tig0032786:+4355 Msa1450080:CDS
TGTGGACTTGGTGAAGCAAT+GGG 0.489096 tig0032786:+5026 Msa1450080:CDS
ATTGGAAGGATTCTTGTCTA+GGG 0.489782 tig0032786:-6958 Msa1450080:intergenic
GCATAAAATATGCAAGGTCC+GGG 0.491454 tig0032786:-7056 Msa1450080:intergenic
AAAAGGGGTTCCCGTGAGGA+AGG 0.495867 tig0032786:+4371 Msa1450080:CDS
GACGCAGACCAGCTGAATGA+AGG 0.498682 tig0032786:+6560 Msa1450080:CDS
ATACAGGGCATCTGGATGTA+AGG 0.499007 tig0032786:+6621 Msa1450080:CDS
GATGCAGTGCAGTTGGATCC+AGG 0.503877 tig0032786:+6680 Msa1450080:CDS
GTATCCGACTGCCGTGCATC+CGG 0.506762 tig0032786:-6647 Msa1450080:intergenic
AGAGGAAGGATTCTTATGTA+AGG 0.509703 tig0032786:-6912 Msa1450080:intergenic
CAAGCGACTAAGAATCTACA+TGG 0.511335 tig0032786:+6485 Msa1450080:CDS
AGTACATTCTTCAAAATGGA+TGG 0.513241 tig0032786:-5083 Msa1450080:intergenic
TTATTCAACATTCCTGTGAA+AGG 0.518920 tig0032786:-7276 Msa1450080:intergenic
AGTGGCCGGGGTTTGAACCC+CGG 0.520773 tig0032786:+7038 Msa1450080:three_prime_UTR
GCATCTAGATGCAGTGCAGT+TGG 0.520962 tig0032786:+6673 Msa1450080:CDS
TGGTCTGCGTCCCTCTGCCA+TGG 0.521247 tig0032786:-6548 Msa1450080:intergenic
TTCAACATTCCTGTGAAAGG+TGG 0.522986 tig0032786:-7273 Msa1450080:intergenic
GGTTGTAAGGTTCTACCTCA+TGG 0.523538 tig0032786:+5128 Msa1450080:CDS
AATGAAGGGGAGTCAAATGC+AGG 0.523780 tig0032786:+6575 Msa1450080:CDS
GATGGTTGCGCAATCGGAGT+AGG 0.526052 tig0032786:-4253 Msa1450080:intergenic
GGAGTAGGACGGTGGCCAAT+CGG 0.530563 tig0032786:-4238 Msa1450080:intergenic
GAGAGAGAGAGGAAGTCGGT+GGG 0.531856 tig0032786:-4295 Msa1450080:intergenic
ATCTATAAAATTGATATGGA+AGG 0.532178 tig0032786:-6783 Msa1450080:intergenic
ACGCAGACCAGCTGAATGAA+GGG 0.538664 tig0032786:+6561 Msa1450080:CDS
TGTTAGTTTCCACCTTTCAC+AGG 0.542839 tig0032786:+7264 Msa1450080:three_prime_UTR
CCAGCTGAGCTAAGCTCACA+AGG 0.543579 tig0032786:+7089 Msa1450080:three_prime_UTR
GTGGACCTTTGACCTTCTCA+CGG 0.544091 tig0032786:-6424 Msa1450080:intergenic
GTCGCTTGTTTCAACTTATG+CGG 0.544680 tig0032786:-6470 Msa1450080:intergenic
AAGGTCCGGGGTTCAAACCC+CGG 0.546686 tig0032786:-7043 Msa1450080:intergenic
CGCACACGATCTCTTCCGAT+TGG 0.547710 tig0032786:+4223 Msa1450080:CDS
TGGAGAATCCCCTTCCTCAC+GGG 0.550577 tig0032786:-4381 Msa1450080:intergenic
TCCAATAGTAGGAGACCTAT+GGG 0.551867 tig0032786:+6975 Msa1450080:three_prime_UTR
AAAGGGGTTCCCGTGAGGAA+GGG 0.562673 tig0032786:+4372 Msa1450080:CDS
TTGTGGACTTGGTGAAGCAA+TGG 0.563332 tig0032786:+5025 Msa1450080:CDS
GCATCTGGATGTAAGGCAGC+CGG 0.564627 tig0032786:+6628 Msa1450080:CDS
TTTGAAGAATGTACTTGAGG+AGG 0.565288 tig0032786:+5091 Msa1450080:CDS
GCGGTAACTGTTCTGTCGAT+AGG 0.571962 tig0032786:-4152 Msa1450080:intergenic
ACGCTACAACACCATGGCAG+AGG 0.576642 tig0032786:+6537 Msa1450080:CDS
ATGTAAGGCAGCCGGATGCA+CGG 0.577781 tig0032786:+6636 Msa1450080:CDS
TGCGAGAAGAGAGATACGAC+TGG 0.579475 tig0032786:-4204 Msa1450080:intergenic
CGCTACAACACCATGGCAGA+GGG 0.584181 tig0032786:+6538 Msa1450080:CDS
ATGCAGGGCAGCTGGATACA+GGG 0.585703 tig0032786:+6606 Msa1450080:CDS
GACAATGCATAAAATATGCA+AGG 0.589572 tig0032786:-7062 Msa1450080:intergenic
TTGGATCCAGGTGTTTCACT+AGG 0.589935 tig0032786:+6692 Msa1450080:CDS
AATACTCAACATTGTTGTAG+AGG 0.592786 tig0032786:-7125 Msa1450080:intergenic
CTATCATTCTCGGTTCCTCA+GGG 0.594726 tig0032786:+5050 Msa1450080:CDS
TCCTAAAGAAAACCGTGAGA+AGG 0.598029 tig0032786:+6412 Msa1450080:CDS
GCAGCCGGATGCACGGCAGT+CGG 0.601262 tig0032786:+6643 Msa1450080:CDS
GATCGAAGAAAAGAATGAGA+TGG 0.601328 tig0032786:-4271 Msa1450080:intergenic
ATAGGTCTCCTACTATTGGA+AGG 0.602195 tig0032786:-6972 Msa1450080:intergenic
ATGGAGAATCCCCTTCCTCA+CGG 0.613119 tig0032786:-4382 Msa1450080:intergenic
ATGCACGGCAGTCGGATACA+GGG 0.614645 tig0032786:+6651 Msa1450080:CDS
GAAAACCGTGAGAAGGTCAA+AGG 0.614702 tig0032786:+6419 Msa1450080:CDS
ATGAAGGGGAGTCAAATGCA+GGG 0.626276 tig0032786:+6576 Msa1450080:CDS
ATGCAGGGGAGCAAGATGCA+GGG 0.636179 tig0032786:+6591 Msa1450080:CDS
CGCAGACCAGCTGAATGAAG+GGG 0.641381 tig0032786:+6562 Msa1450080:CDS
AAGGGTTCTGGTCAGAAAAG+GGG 0.652348 tig0032786:+4356 Msa1450080:CDS
CATAAAATATGCAAGGTCCG+GGG 0.657923 tig0032786:-7055 Msa1450080:intergenic
GCGAGAAGAGAGATACGACT+GGG 0.658917 tig0032786:-4203 Msa1450080:intergenic
GTTGCGCAATCGGAGTAGGA+CGG 0.665262 tig0032786:-4249 Msa1450080:intergenic
TGAAGGGGAGTCAAATGCAG+GGG 0.669223 tig0032786:+6577 Msa1450080:CDS
GCTTTACCTAGTGAAACACC+TGG 0.684772 tig0032786:-6698 Msa1450080:intergenic
GATGGAAATTTGACTCCCTG+AGG 0.694236 tig0032786:-5065 Msa1450080:intergenic
GCGCAATCGGAGTAGGACGG+TGG 0.704019 tig0032786:-4246 Msa1450080:intergenic
TCAGAAAAGGGGTTCCCGTG+AGG 0.706408 tig0032786:+4367 Msa1450080:CDS
GTGATGACGCTACAACACCA+TGG 0.716250 tig0032786:+6531 Msa1450080:CDS
GTTGTAGCGTCATCACTGAG+AGG 0.716312 tig0032786:-6524 Msa1450080:intergenic
GTGGACTTGGTGAAGCAATG+GGG 0.770343 tig0032786:+5027 Msa1450080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTGTAATTAACAATTA+AGG - tig0032786:4432-4451 Msa1450080:intergenic 10.0%
!! TTAAATCTATAAAATTGATA+TGG - tig0032786:6790-6809 Msa1450080:intergenic 10.0%
!!! ATATCAATTTTATAGATTTA+AGG + tig0032786:6789-6808 Msa1450080:three_prime_UTR 10.0%
!!! TAAATTTTTTATTTTGAAAG+AGG - tig0032786:6933-6952 Msa1450080:intergenic 10.0%
!!! ATTTTTAAGAATGAAATGTT+TGG - tig0032786:5259-5278 Msa1450080:intergenic 15.0%
!!! CACTTTAGTATATTTTATTA+CGG - tig0032786:5345-5364 Msa1450080:intergenic 15.0%
!! AATAAAATATACTAAAGTGA+CGG + tig0032786:5346-5365 Msa1450080:intron 15.0%
!!! TAACAAATGTATTTTTGTTT+TGG + tig0032786:6241-6260 Msa1450080:intron 15.0%
!! AGACTTACAAAAATTGAAAA+TGG - tig0032786:4404-4423 Msa1450080:intergenic 20.0%
!! AATTGAAAAACACAATCAAA+TGG - tig0032786:4497-4516 Msa1450080:intergenic 20.0%
!! CTTTACGAATAGATTAAATA+TGG + tig0032786:4658-4677 Msa1450080:intron 20.0%
!!! TAAGAATAATATTGGTTGTA+AGG + tig0032786:5115-5134 Msa1450080:CDS 20.0%
!! TCTATAGAAATGTTAGAAAT+TGG + tig0032786:5195-5214 Msa1450080:intron 20.0%
!!! CTTTTTTACAAAAAATTGAG+AGG - tig0032786:5319-5338 Msa1450080:intergenic 20.0%
!!! ACAACTTTAATTTAGTTTCA+GGG + tig0032786:5569-5588 Msa1450080:intron 20.0%
!! AAATTTATAGGATCATCTAA+TGG - tig0032786:5937-5956 Msa1450080:intergenic 20.0%
!! ATTAGATGATCCTATAAATT+TGG + tig0032786:5936-5955 Msa1450080:intron 20.0%
!! ATCATTTACTCCAAATTTAT+AGG - tig0032786:5949-5968 Msa1450080:intergenic 20.0%
!!! TCTTTTACATATTGAATTTG+AGG + tig0032786:6193-6212 Msa1450080:intron 20.0%
!!! AAATGTATTTTTGTTTTGGT+TGG + tig0032786:6245-6264 Msa1450080:intron 20.0%
!! TTAGAACATTAATTAGTGTA+TGG - tig0032786:6304-6323 Msa1450080:intergenic 20.0%
!!! CTATATTCTCTTATGTTTTT+TGG + tig0032786:6377-6396 Msa1450080:intron 20.0%
!! TTAAGATTATGATCATGATT+AGG - tig0032786:6725-6744 Msa1450080:intergenic 20.0%
!!! AACTCTTAAGATTATTTTCA+AGG + tig0032786:6743-6762 Msa1450080:exon 20.0%
!!! ACTCTTAAGATTATTTTCAA+GGG + tig0032786:6744-6763 Msa1450080:exon 20.0%
!! ATCTATAAAATTGATATGGA+AGG - tig0032786:6786-6805 Msa1450080:intergenic 20.0%
!!! AATTTTATAGATTTAAGGCT+GGG + tig0032786:6794-6813 Msa1450080:three_prime_UTR 20.0%
!!! TTTTTTATTTTGAAAGAGGA+AGG - tig0032786:6929-6948 Msa1450080:intergenic 20.0%
!!! GTGCAGTTAATTATTTTTTT+GGG - tig0032786:7007-7026 Msa1450080:intergenic 20.0%
!!! GCATATAATTTTCAATTTTG+CGG + tig0032786:7204-7223 Msa1450080:three_prime_UTR 20.0%
! ATTCCATACATGTATATTTG+TGG + tig0032786:4454-4473 Msa1450080:intron 25.0%
! TAACCACAAATATACATGTA+TGG - tig0032786:4460-4479 Msa1450080:intergenic 25.0%
! ACATGTATATTTGTGGTTAT+TGG + tig0032786:4461-4480 Msa1450080:intron 25.0%
! CAAATTATTCAATGTGATTG+TGG + tig0032786:4543-4562 Msa1450080:intron 25.0%
!!! TCAAGTACTGATTGAATTTT+GGG + tig0032786:4726-4745 Msa1450080:intron 25.0%
!!! AAGTTGGATTTTTTTTGATC+TGG + tig0032786:4785-4804 Msa1450080:intron 25.0%
!!! AGTTGGATTTTTTTTGATCT+GGG + tig0032786:4786-4805 Msa1450080:intron 25.0%
! AGATTAGGACACATTATATT+AGG + tig0032786:4815-4834 Msa1450080:intron 25.0%
! GAATATTGATAAGACTTTGA+AGG + tig0032786:4849-4868 Msa1450080:CDS 25.0%
!!! GGTTAGTTTGATAATTATCA+TGG + tig0032786:4912-4931 Msa1450080:intron 25.0%
!!! GATAATTATCATGGTTTTAC+TGG + tig0032786:4921-4940 Msa1450080:intron 25.0%
!!! TACTTCACTGATTTTGATTT+TGG + tig0032786:4986-5005 Msa1450080:intron 25.0%
!!! GATTTTGATTTTGGTCTTTT+AGG + tig0032786:4995-5014 Msa1450080:intron 25.0%
!! TCTATAGAAGCTTTAATCAA+TGG - tig0032786:5183-5202 Msa1450080:intergenic 25.0%
! ATTCACAATTAAAGCATACA+TGG + tig0032786:5391-5410 Msa1450080:intron 25.0%
! CTGAAACTAAATTAAAGTTG+TGG - tig0032786:5570-5589 Msa1450080:intergenic 25.0%
!! CACAACTTTAATTTAGTTTC+AGG + tig0032786:5568-5587 Msa1450080:intron 25.0%
! TAATACACCAAACATAGTAA+GGG - tig0032786:5719-5738 Msa1450080:intergenic 25.0%
! ATAATACACCAAACATAGTA+AGG - tig0032786:5720-5739 Msa1450080:intergenic 25.0%
! TTGGAGTAAATGATTCAATT+CGG + tig0032786:5955-5974 Msa1450080:intron 25.0%
! ATTAGCACTATAAGATCAAT+TGG - tig0032786:6111-6130 Msa1450080:intergenic 25.0%
!!! CAATTTTATAGATTTAAGGC+TGG + tig0032786:6793-6812 Msa1450080:three_prime_UTR 25.0%
! TTCTTATGTAAGGAATAGTT+GGG - tig0032786:6905-6924 Msa1450080:intergenic 25.0%
! ATTCTTATGTAAGGAATAGT+TGG - tig0032786:6906-6925 Msa1450080:intergenic 25.0%
!!! GGTGCAGTTAATTATTTTTT+TGG - tig0032786:7008-7027 Msa1450080:intergenic 25.0%
!! ATAATTTTCAATTTTGCGGA+TGG + tig0032786:7208-7227 Msa1450080:three_prime_UTR 25.0%
!! TTTTCAATTGACTCTGATTG+TGG + tig0032786:4508-4527 Msa1450080:intron 30.0%
!!! GTCAAGTACTGATTGAATTT+TGG + tig0032786:4725-4744 Msa1450080:intron 30.0%
!!! CAAGTACTGATTGAATTTTG+GGG + tig0032786:4727-4746 Msa1450080:intron 30.0%
!! GATTTGTGGTTTTTGAAAGT+TGG + tig0032786:4769-4788 Msa1450080:intron 30.0%
TCACTTTGATCAATATTGTC+AGG + tig0032786:4891-4910 Msa1450080:CDS 30.0%
AGTACATTCTTCAAAATGGA+TGG - tig0032786:5086-5105 Msa1450080:intergenic 30.0%
CTCAAGTACATTCTTCAAAA+TGG - tig0032786:5090-5109 Msa1450080:intergenic 30.0%
!! CATTTTGAAGAATGTACTTG+AGG + tig0032786:5088-5107 Msa1450080:CDS 30.0%
GAGGAGGTTAAGAATAATAT+TGG + tig0032786:5107-5126 Msa1450080:CDS 30.0%
TCATGGCAATCTTGAAAAAT+GGG + tig0032786:5145-5164 Msa1450080:CDS 30.0%
GAAAGAGTGATTTCTTTGAA+TGG + tig0032786:5293-5312 Msa1450080:intron 30.0%
TATGAACACAGTATGAAGTT+AGG + tig0032786:5428-5447 Msa1450080:intron 30.0%
TGTTTATTCTAAATGCAGAG+TGG + tig0032786:5455-5474 Msa1450080:intron 30.0%
GTTTATTCTAAATGCAGAGT+GGG + tig0032786:5456-5475 Msa1450080:intron 30.0%
AAATGAGTGAACAACAGAAA+GGG - tig0032786:5485-5504 Msa1450080:intergenic 30.0%
! GAACTTCTAATCAAGAGTAT+TGG + tig0032786:5643-5662 Msa1450080:intron 30.0%
AAAATGACCCTTACTATGTT+TGG + tig0032786:5709-5728 Msa1450080:intron 30.0%
!! TTGTAACTAGCTTTCAATTC+AGG + tig0032786:5764-5783 Msa1450080:intron 30.0%
ATGAATGATTGAAGTCCTTA+AGG + tig0032786:6268-6287 Msa1450080:intron 30.0%
!! TATGCGAACTTTAAAACAGA+CGG - tig0032786:6331-6350 Msa1450080:intergenic 30.0%
!!! TATGTTTTTTGGACAGATCA+TGG + tig0032786:6388-6407 Msa1450080:intron 30.0%
!! TAAGATTATTTTCAAGGGCA+AGG + tig0032786:6749-6768 Msa1450080:exon 30.0%
!! AAGATTATTTTCAAGGGCAA+GGG + tig0032786:6750-6769 Msa1450080:exon 30.0%
! TAATTAACTGCACCTCTTTT+TGG + tig0032786:7014-7033 Msa1450080:three_prime_UTR 30.0%
GACAATGCATAAAATATGCA+AGG - tig0032786:7065-7084 Msa1450080:intergenic 30.0%
AATACTCAACATTGTTGTAG+AGG - tig0032786:7128-7147 Msa1450080:intergenic 30.0%
! GTTGAGTATTGTCAACTATT+TGG + tig0032786:7138-7157 Msa1450080:three_prime_UTR 30.0%
TGTTGCAATTACTATCTTAG+TGG + tig0032786:7182-7201 Msa1450080:three_prime_UTR 30.0%
TTATTCAACATTCCTGTGAA+AGG - tig0032786:7279-7298 Msa1450080:intergenic 30.0%
GTTGAATAAATTGTGCATGT+TGG + tig0032786:7290-7309 Msa1450080:three_prime_UTR 30.0%
GATCGAAGAAAAGAATGAGA+TGG - tig0032786:4274-4293 Msa1450080:intergenic 35.0%
CTATTCGATTTCAGGATTCT+CGG + tig0032786:4329-4348 Msa1450080:CDS 35.0%
TATTCGATTTCAGGATTCTC+GGG + tig0032786:4330-4349 Msa1450080:CDS 35.0%
!! GAATTGACTTGTGTTTGACT+TGG + tig0032786:4703-4722 Msa1450080:intron 35.0%
!! ATTGAATTTTGGGGATCTGA+AGG + tig0032786:4736-4755 Msa1450080:intron 35.0%
!! TTGAATTTTGGGGATCTGAA+GGG + tig0032786:4737-4756 Msa1450080:intron 35.0%
!! GTCTTTTAGGAACCTTATTG+TGG + tig0032786:5008-5027 Msa1450080:intron 35.0%
TTTGAAGAATGTACTTGAGG+AGG + tig0032786:5091-5110 Msa1450080:CDS 35.0%
!!! CATTTTTCAAGATTGCCATG+AGG - tig0032786:5146-5165 Msa1450080:intergenic 35.0%
CTCATGGCAATCTTGAAAAA+TGG + tig0032786:5144-5163 Msa1450080:CDS 35.0%
CAAATGAGTGAACAACAGAA+AGG - tig0032786:5486-5505 Msa1450080:intergenic 35.0%
! TTGCTGAATTTTGTTCTCAC+AGG + tig0032786:5599-5618 Msa1450080:intron 35.0%
AGTCATAAACTGTTTGTGCA+TGG + tig0032786:5674-5693 Msa1450080:intron 35.0%
!! AACTAGCTTTCAATTCAGGT+AGG + tig0032786:5768-5787 Msa1450080:intron 35.0%
GCAAATGCACATGCAAATAT+AGG + tig0032786:5806-5825 Msa1450080:intron 35.0%
GTCATCACTATGTGATTTCA+TGG - tig0032786:5988-6007 Msa1450080:intergenic 35.0%
TATGGTGTACATGTTCCTTA+AGG - tig0032786:6286-6305 Msa1450080:intergenic 35.0%
!!! ATCATCCACTTTTTCATCTG+TGG - tig0032786:6446-6465 Msa1450080:intergenic 35.0%
AGAGGAAGGATTCTTATGTA+AGG - tig0032786:6915-6934 Msa1450080:intergenic 35.0%
ATTGGAAGGATTCTTGTCTA+GGG - tig0032786:6961-6980 Msa1450080:intergenic 35.0%
TATTGGAAGGATTCTTGTCT+AGG - tig0032786:6962-6981 Msa1450080:intergenic 35.0%
ACAAGAATCCTTCCAATAGT+AGG + tig0032786:6964-6983 Msa1450080:three_prime_UTR 35.0%
!!! TTTTTTGGGTGTATACCCAT+AGG - tig0032786:6993-7012 Msa1450080:intergenic 35.0%
TGCATAAAATATGCAAGGTC+CGG - tig0032786:7060-7079 Msa1450080:intergenic 35.0%
CTCTCTCTCTATTCGATTTC+AGG + tig0032786:4321-4340 Msa1450080:CDS 40.0%
! AAAGGGTTCTGGTCAGAAAA+GGG + tig0032786:4355-4374 Msa1450080:CDS 40.0%
!!! AAGGGTGTTTTGTGGATTTG+TGG + tig0032786:4755-4774 Msa1450080:intron 40.0%
TAGGAACCTTATTGTGGACT+TGG + tig0032786:5014-5033 Msa1450080:intron 40.0%
TCTTGAAAAATGGGATGTGC+AGG + tig0032786:5154-5173 Msa1450080:CDS 40.0%
!! AGTGACGGATTTCAGATTTG+TGG + tig0032786:5361-5380 Msa1450080:intron 40.0%
TGTTCACTCATTTGTGCAGA+TGG + tig0032786:5492-5511 Msa1450080:intron 40.0%
CAAGACGATCATAGAGAAGA+AGG + tig0032786:5856-5875 Msa1450080:intron 40.0%
ACGATCATAGAGAAGAAGGA+AGG + tig0032786:5860-5879 Msa1450080:intron 40.0%
CGATCATAGAGAAGAAGGAA+GGG + tig0032786:5861-5880 Msa1450080:intron 40.0%
GATCATAGAGAAGAAGGAAG+GGG + tig0032786:5862-5881 Msa1450080:intron 40.0%
AGTATGCGCAGGAGAAAATT+CGG + tig0032786:5906-5925 Msa1450080:intron 40.0%
CTGAAGCATAAGAGCAAGTA+TGG - tig0032786:6141-6160 Msa1450080:intergenic 40.0%
AGATGAGATGAAATCGACGT+AGG + tig0032786:6217-6236 Msa1450080:intron 40.0%
TCCTAAAGAAAACCGTGAGA+AGG + tig0032786:6412-6431 Msa1450080:CDS 40.0%
! ACCTTCTCACGGTTTTCTTT+AGG - tig0032786:6416-6435 Msa1450080:intergenic 40.0%
AAGGTCCACAGATGAAAAAG+TGG + tig0032786:6438-6457 Msa1450080:CDS 40.0%
GTCGCTTGTTTCAACTTATG+CGG - tig0032786:6473-6492 Msa1450080:intergenic 40.0%
CAAGCGACTAAGAATCTACA+TGG + tig0032786:6485-6504 Msa1450080:CDS 40.0%
! TCTGTCCTTTTTCTTGTACG+AGG + tig0032786:6864-6883 Msa1450080:three_prime_UTR 40.0%
ATAGGTCTCCTACTATTGGA+AGG - tig0032786:6975-6994 Msa1450080:intergenic 40.0%
TTCCAATAGTAGGAGACCTA+TGG + tig0032786:6974-6993 Msa1450080:three_prime_UTR 40.0%
TCCAATAGTAGGAGACCTAT+GGG + tig0032786:6975-6994 Msa1450080:three_prime_UTR 40.0%
CATAAAATATGCAAGGTCCG+GGG - tig0032786:7058-7077 Msa1450080:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG - tig0032786:7059-7078 Msa1450080:intergenic 40.0%
! TGTTAGTTTCCACCTTTCAC+AGG + tig0032786:7264-7283 Msa1450080:three_prime_UTR 40.0%
TTCAACATTCCTGTGAAAGG+TGG - tig0032786:7276-7295 Msa1450080:intergenic 40.0%
TTCACGAAAGAGAGAGAGAG+AGG - tig0032786:4179-4198 Msa0922400:intergenic 45.0%
GAATGAGATGGTTGCGCAAT+CGG - tig0032786:4262-4281 Msa1450080:intergenic 45.0%
TTTCAGGATTCTCGGGAGAA+AGG + tig0032786:4337-4356 Msa1450080:CDS 45.0%
TTCAGGATTCTCGGGAGAAA+GGG + tig0032786:4338-4357 Msa1450080:CDS 45.0%
! GAAAGGGTTCTGGTCAGAAA+AGG + tig0032786:4354-4373 Msa1450080:CDS 45.0%
! AAGGGTTCTGGTCAGAAAAG+GGG + tig0032786:4356-4375 Msa1450080:CDS 45.0%
!! ACTCTGATTGTGGCTTGTTG+TGG + tig0032786:4518-4537 Msa1450080:intron 45.0%
! TGATCTGGGTGTTGCAGATT+AGG + tig0032786:4800-4819 Msa1450080:intron 45.0%
GCTTCACCAAGTCCACAATA+AGG - tig0032786:5023-5042 Msa1450080:intergenic 45.0%
!! TTGTGGACTTGGTGAAGCAA+TGG + tig0032786:5025-5044 Msa1450080:CDS 45.0%
!! TGTGGACTTGGTGAAGCAAT+GGG + tig0032786:5026-5045 Msa1450080:CDS 45.0%
AGCAATGGGGCTATCATTCT+CGG + tig0032786:5040-5059 Msa1450080:CDS 45.0%
CTATCATTCTCGGTTCCTCA+GGG + tig0032786:5050-5069 Msa1450080:CDS 45.0%
GATGGAAATTTGACTCCCTG+AGG - tig0032786:5068-5087 Msa1450080:intergenic 45.0%
GGTTGTAAGGTTCTACCTCA+TGG + tig0032786:5128-5147 Msa1450080:CDS 45.0%
GAAAACCGTGAGAAGGTCAA+AGG + tig0032786:6419-6438 Msa1450080:CDS 45.0%
AATGAAGGGGAGTCAAATGC+AGG + tig0032786:6575-6594 Msa1450080:CDS 45.0%
ATGAAGGGGAGTCAAATGCA+GGG + tig0032786:6576-6595 Msa1450080:CDS 45.0%
! ATACAGGGCATCTGGATGTA+AGG + tig0032786:6621-6640 Msa1450080:CDS 45.0%
TTGGATCCAGGTGTTTCACT+AGG + tig0032786:6692-6711 Msa1450080:CDS 45.0%
GCTTTACCTAGTGAAACACC+TGG - tig0032786:6701-6720 Msa1450080:intergenic 45.0%
ACCCATAGGTCTCCTACTAT+TGG - tig0032786:6979-6998 Msa1450080:intergenic 45.0%
!! ACTGCACCTCTTTTTGGTAG+TGG + tig0032786:7020-7039 Msa1450080:three_prime_UTR 45.0%
GCGGTAACTGTTCTGTCGAT+AGG - tig0032786:4155-4174 Msa0922400:intergenic 50.0%
GAAAGAGAGAGAGAGAGGAG+CGG - tig0032786:4174-4193 Msa0922400:intergenic 50.0%
GCGAGAAGAGAGATACGACT+GGG - tig0032786:4206-4225 Msa0922400:intergenic 50.0%
TGCGAGAAGAGAGATACGAC+TGG - tig0032786:4207-4226 Msa0922400:intergenic 50.0%
GAGAGAGAGAGAGAGGAAGT+CGG - tig0032786:4302-4321 Msa1450080:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - tig0032786:4307-4326 Msa1450080:intergenic 50.0%
ATTCTCGGGAGAAAGGGTTC+TGG + tig0032786:4344-4363 Msa1450080:CDS 50.0%
ATGGAGAATCCCCTTCCTCA+CGG - tig0032786:4385-4404 Msa1450080:intergenic 50.0%
!!! GGGATCTGAAGGGTGTTTTG+TGG + tig0032786:4747-4766 Msa1450080:intron 50.0%
!! GTGGACTTGGTGAAGCAATG+GGG + tig0032786:5027-5046 Msa1450080:CDS 50.0%
GCTATCATTCTCGGTTCCTC+AGG + tig0032786:5049-5068 Msa1450080:CDS 50.0%
! AAGGGGTCGTGTTTCTGATG+TGG + tig0032786:5879-5898 Msa1450080:intron 50.0%
! AGGGGTCGTGTTTCTGATGT+GGG + tig0032786:5880-5899 Msa1450080:intron 50.0%
GATGTGGGAATAGTATGCGC+AGG + tig0032786:5895-5914 Msa1450080:intron 50.0%
GTGGACCTTTGACCTTCTCA+CGG - tig0032786:6427-6446 Msa1450080:intergenic 50.0%
GTTGTAGCGTCATCACTGAG+AGG - tig0032786:6527-6546 Msa1450080:intergenic 50.0%
! GTGATGACGCTACAACACCA+TGG + tig0032786:6531-6550 Msa1450080:CDS 50.0%
ACGCAGACCAGCTGAATGAA+GGG + tig0032786:6561-6580 Msa1450080:CDS 50.0%
TTGACTCCCCTTCATTCAGC+TGG - tig0032786:6571-6590 Msa1450080:intergenic 50.0%
TGAAGGGGAGTCAAATGCAG+GGG + tig0032786:6577-6596 Msa1450080:CDS 50.0%
GCATCTAGATGCAGTGCAGT+TGG + tig0032786:6673-6692 Msa1450080:CDS 50.0%
GAGCGCCTCGTACAAGAAAA+AGG - tig0032786:6872-6891 Msa1450080:intergenic 50.0%
!!! CACCTCTTTTTGGTAGTGGC+CGG + tig0032786:7024-7043 Msa1450080:three_prime_UTR 50.0%
!!! ACCTCTTTTTGGTAGTGGCC+GGG + tig0032786:7025-7044 Msa1450080:three_prime_UTR 50.0%
TGAGCTTAGCTCAGCTGGTT+GGG - tig0032786:7087-7106 Msa1450080:intergenic 50.0%
CGCACACGATCTCTTCCGAT+TGG + tig0032786:4223-4242 Msa1450080:CDS 55.0%
GTTGCGCAATCGGAGTAGGA+CGG - tig0032786:4252-4271 Msa1450080:intergenic 55.0%
GATGGTTGCGCAATCGGAGT+AGG - tig0032786:4256-4275 Msa1450080:intergenic 55.0%
GAGAGAGAGAGGAAGTCGGT+GGG - tig0032786:4298-4317 Msa1450080:intergenic 55.0%
AGAGAGAGAGAGGAAGTCGG+TGG - tig0032786:4299-4318 Msa1450080:intergenic 55.0%
TCAGAAAAGGGGTTCCCGTG+AGG + tig0032786:4367-4386 Msa1450080:CDS 55.0%
AAAAGGGGTTCCCGTGAGGA+AGG + tig0032786:4371-4390 Msa1450080:CDS 55.0%
AAAGGGGTTCCCGTGAGGAA+GGG + tig0032786:4372-4391 Msa1450080:CDS 55.0%
TGGAGAATCCCCTTCCTCAC+GGG - tig0032786:4384-4403 Msa1450080:intergenic 55.0%
ACGCTACAACACCATGGCAG+AGG + tig0032786:6537-6556 Msa1450080:CDS 55.0%
CGCTACAACACCATGGCAGA+GGG + tig0032786:6538-6557 Msa1450080:CDS 55.0%
GACGCAGACCAGCTGAATGA+AGG + tig0032786:6560-6579 Msa1450080:CDS 55.0%
CGCAGACCAGCTGAATGAAG+GGG + tig0032786:6562-6581 Msa1450080:CDS 55.0%
AATGCAGGGGAGCAAGATGC+AGG + tig0032786:6590-6609 Msa1450080:CDS 55.0%
ATGCAGGGGAGCAAGATGCA+GGG + tig0032786:6591-6610 Msa1450080:CDS 55.0%
ATGCAGGGCAGCTGGATACA+GGG + tig0032786:6606-6625 Msa1450080:CDS 55.0%
GCATCTGGATGTAAGGCAGC+CGG + tig0032786:6628-6647 Msa1450080:CDS 55.0%
ATGTAAGGCAGCCGGATGCA+CGG + tig0032786:6636-6655 Msa1450080:CDS 55.0%
ATGCACGGCAGTCGGATACA+GGG + tig0032786:6651-6670 Msa1450080:CDS 55.0%
GATGCAGTGCAGTTGGATCC+AGG + tig0032786:6680-6699 Msa1450080:CDS 55.0%
!!! CCTCTTTTTGGTAGTGGCCG+GGG + tig0032786:7026-7045 Msa1450080:three_prime_UTR 55.0%
GTGAGCTTAGCTCAGCTGGT+TGG - tig0032786:7088-7107 Msa1450080:intergenic 55.0%
CCTTGTGAGCTTAGCTCAGC+TGG - tig0032786:7092-7111 Msa1450080:intergenic 55.0%
CCAGCTGAGCTAAGCTCACA+AGG + tig0032786:7089-7108 Msa1450080:three_prime_UTR 55.0%
GGAGTAGGACGGTGGCCAAT+CGG - tig0032786:4241-4260 Msa1450080:intergenic 60.0%
AAGGGGTTCCCGTGAGGAAG+GGG + tig0032786:4373-4392 Msa1450080:CDS 60.0%
GATGCAGGGCAGCTGGATAC+AGG + tig0032786:6605-6624 Msa1450080:CDS 60.0%
! GCAGCTGGATACAGGGCATC+TGG + tig0032786:6613-6632 Msa1450080:CDS 60.0%
GTATCCGACTGCCGTGCATC+CGG - tig0032786:6650-6669 Msa1450080:intergenic 60.0%
GATGCACGGCAGTCGGATAC+AGG + tig0032786:6650-6669 Msa1450080:CDS 60.0%
CCCCGGCCACTACCAAAAAG+AGG - tig0032786:7029-7048 Msa1450080:intergenic 60.0%
AAGGTCCGGGGTTCAAACCC+CGG - tig0032786:7046-7065 Msa1450080:intergenic 60.0%
GCGCAATCGGAGTAGGACGG+TGG - tig0032786:4249-4268 Msa1450080:intergenic 65.0%
TGGTCTGCGTCCCTCTGCCA+TGG - tig0032786:6551-6570 Msa1450080:intergenic 65.0%
GGAGCAAGATGCAGGGCAGC+TGG + tig0032786:6598-6617 Msa1450080:CDS 65.0%
AGTGGCCGGGGTTTGAACCC+CGG + tig0032786:7038-7057 Msa1450080:three_prime_UTR 65.0%
GCAGCCGGATGCACGGCAGT+CGG + tig0032786:6643-6662 Msa1450080:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0032786 gene 4149 7313 4149 ID=Msa1450080;Name=Msa1450080
tig0032786 mRNA 4149 7313 4149 ID=Msa1450080-mRNA-1;Parent=Msa1450080;Name=Msa1450080-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0.66|0.75|1|1|1|4|562|289
tig0032786 exon 4149 4415 4149 ID=Msa1450080-mRNA-1:exon:17270;Parent=Msa1450080-mRNA-1
tig0032786 exon 4817 4912 4817 ID=Msa1450080-mRNA-1:exon:17271;Parent=Msa1450080-mRNA-1
tig0032786 exon 5017 5175 5017 ID=Msa1450080-mRNA-1:exon:17272;Parent=Msa1450080-mRNA-1
tig0032786 exon 6404 7313 6404 ID=Msa1450080-mRNA-1:exon:17273;Parent=Msa1450080-mRNA-1
tig0032786 CDS 4149 4415 4149 ID=Msa1450080-mRNA-1:cds;Parent=Msa1450080-mRNA-1
tig0032786 CDS 4817 4912 4817 ID=Msa1450080-mRNA-1:cds;Parent=Msa1450080-mRNA-1
tig0032786 CDS 5017 5175 5017 ID=Msa1450080-mRNA-1:cds;Parent=Msa1450080-mRNA-1
tig0032786 CDS 6404 6751 6404 ID=Msa1450080-mRNA-1:cds;Parent=Msa1450080-mRNA-1
tig0032786 three_prime_UTR 6752 7313 6752 ID=Msa1450080-mRNA-1:three_prime_utr;Parent=Msa1450080-mRNA-1
Gene Sequence

>Msa1450080

ACTCCTATCGACAGAACAGTTACCGCTCCTCTCTCTCTCTCTTTCGTGAACTCTCCCAGTCGTATCTCTCTTCTCGCACACGATCTCTTCCGATTGGCCACCGTCCTACTCCGATTGCGCAACCATCTCATTCTTTTCTTCGATCACCCACCGACTTCCTCTCTCTCTCTCTCTCTCTCTCTATTCGATTTCAGGATTCTCGGGAGAAAGGGTTCTGGTCAGAAAAGGGGTTCCCGTGAGGAAGGGGATTCTCCATTTTCAATTTTTATTAGGACACATTATATTAGGAAAGCTCATGAGAATATTGATAAGACTTTGAAGGCTTCTGAGATTATATTGTCTCACTTTGATCAATATTGTCAGGAACCTTATTGTGGACTTGGTGAAGCAATGGGGCTATCATTCTCGGTTCCTCAGGGAGTCAAATTTCCATCCATTTTGAAGAATGTACTTGAGGAGGTTAAGAATAATATTGGTTGTAAGGTTCTACCTCATGGCAATCTTGAAAAATGGGATGTGCAGATCATGGCTCCTAAAGAAAACCGTGAGAAGGTCAAAGGTCCACAGATGAAAAAGTGGATGATTATGCCGCATAAGTTGAAACAAGCGACTAAGAATCTACATGGATTAGCTTTGAAGTCGCCTCTCAGTGATGACGCTACAACACCATGGCAGAGGGACGCAGACCAGCTGAATGAAGGGGAGTCAAATGCAGGGGAGCAAGATGCAGGGCAGCTGGATACAGGGCATCTGGATGTAAGGCAGCCGGATGCACGGCAGTCGGATACAGGGCATCTAGATGCAGTGCAGTTGGATCCAGGTGTTTCACTAGGTAAAGCACCTAATCATGATCATAATCTTAACTCTTAA
Protein sequence

>Msa1450080

TPIDRTVTAPLSLSFVNSPSRISLLAHDLFRLATVLLRLRNHLILFFDHPPTSSLSLSLSLFDFRILGRKGSGQKRGSREEGDSPFSIFIRTHYIRKAHENIDKTLKASEIILSHFDQYCQEPYCGLGEAMGLSFSVPQGVKFPSILKNVLEEVKNNIGCKVLPHGNLEKWDVQIMAPKENREKVKGPQMKKWMIMPHKLKQATKNLHGLALKSPLSDDATTPWQRDADQLNEGESNAGEQDAGQLDTGHLDVRQPDARQSDTGHLDAVQLDPGVSLGKAPNHDHNLNS*