AlfalfaGEDB | Msa0288570

M. sativa cultivar zhongmu-4 / Msa0288570

KEGG: K21751
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0288570	XP_024632301.1	82.888	187	1	1	1	187	1	156	5.25e-104	305

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0288570	sp\|P49592\|NC2B_ARATH	66.486	185	29	2	1	183	1	154	1.40e-80	239

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0288570	I3S9B6	82.888	187	1	1	1	187	1	156	2.51e-104	305

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TFs/TRs:

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Gene ID	Type	Classification
Msa0288570	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0288560	Msa0288570	0.959995	4.645971e-118	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0288570	MtrunA17_Chr2g0307551	82.888	187	1	1	1	187	1	156	4.83e-108	305
Msa0288570	MtrunA17_Chr4g0062271	70.652	184	23	1	1	184	1	153	3.05e-90	260
Msa0288570	MtrunA17_Chr1g0185911	40.449	89	51	1	8	94	26	114	4.80e-18	77.4
Msa0288570	MtrunA17_Chr4g0076381	39.241	79	48	0	11	89	58	136	6.58e-17	75.9
Msa0288570	MtrunA17_Chr1g0195851	37.179	78	49	0	12	89	37	114	7.66e-17	74.7
Msa0288570	MtrunA17_Chr7g0263601	37.500	80	50	0	12	91	27	106	3.44e-16	72.4
Msa0288570	MtrunA17_Chr1g0191981	35.443	79	51	0	13	91	6	84	5.23e-16	70.9
Msa0288570	MtrunA17_Chr1g0165041	33.333	81	54	0	11	91	6	86	8.52e-16	72.0
Msa0288570	MtrunA17_Chr3g0102351	37.179	78	49	0	12	89	28	105	1.16e-15	71.6
Msa0288570	MtrunA17_Chr5g0446491	34.118	85	56	0	7	91	19	103	2.58e-15	70.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0288570	AT5G23090.5	66.486	185	29	2	1	183	1	154	1.42e-81	239
Msa0288570	AT5G23090.2	66.486	185	29	2	1	183	1	154	1.42e-81	239
Msa0288570	AT5G23090.1	66.486	185	29	2	1	183	1	154	1.42e-81	239
Msa0288570	AT5G23090.4	65.946	185	29	2	1	183	1	153	1.24e-80	236
Msa0288570	AT5G08190.1	68.786	173	23	1	1	173	1	142	1.75e-72	216
Msa0288570	AT5G08190.2	68.208	173	23	1	1	173	1	141	1.84e-71	214
Msa0288570	AT5G23090.3	59.459	185	29	3	1	183	1	141	1.54e-67	203
Msa0288570	AT2G38880.11	34.653	101	62	2	12	108	22	122	6.86e-18	77.0
Msa0288570	AT2G38880.8	34.653	101	62	2	12	108	22	122	6.86e-18	77.0
Msa0288570	AT2G38880.4	36.471	85	52	1	12	94	22	106	1.79e-17	74.7

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Find 74 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCATTCACTACTGTGC+TGG	0.521490	2_3:+33928219	Msa0288570:three_prime_UTR
AAAGAGAAGATGAAGAGATG+AGG	0.525840	2_3:-33924784	None:intergenic
AAAGCTTTCAATACATGCTC+AGG	0.483166	2_3:-33926200	None:intergenic
AAAGTTAGTTCTCTAAACTT+TGG	0.415113	2_3:-33928120	None:intergenic
AAATGAAGTATGTAACAGAG+AGG	0.704349	2_3:+33926160	Msa0288570:CDS
AACATTCAATTAACAAATCC+TGG	0.352577	2_3:-33925250	None:intergenic
AAGAGAAGATGAAGAGATGA+GGG	0.481364	2_3:-33924783	None:intergenic
AAGCATTAGCAGAGCAGCAA+AGG	0.547129	2_3:+33928032	Msa0288570:CDS
AAGTTAATGTAATTAATTAA+TGG	0.241152	2_3:+33928292	Msa0288570:three_prime_UTR
AAGTTTAGAGAACTAACTTT+AGG	0.270141	2_3:+33928123	Msa0288570:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATTTGAGGATGAGAAAAA+AGG	-	chr2_3:33924897-33924916	None:intergenic	30.0%
TTCTCATCCTCAAATTCAAT+CGG	+	chr2_3:33924900-33924919	Msa0288570:five_prime_UTR	30.0%
GATTTAGCAACAATATCCAT+AGG	-	chr2_3:33925043-33925062	None:intergenic	30.0%
GGATATTGTTGCTAAATCAA+AGG	+	chr2_3:33925045-33925064	Msa0288570:CDS	30.0%
TAGTGATTAATAATTGCCCT+TGG	+	chr2_3:33925383-33925402	Msa0288570:intron	30.0%
TTATGAACACCACCAAAAAT+CGG	-	chr2_3:33925522-33925541	None:intergenic	30.0%
ATTGATCTCAAAACATGTGA+CGG	-	chr2_3:33925667-33925686	None:intergenic	30.0%
AAATGAAGTATGTAACAGAG+AGG	+	chr2_3:33926160-33926179	Msa0288570:CDS	30.0%
AGCATGTATTGAAAGCTTTA+GGG	+	chr2_3:33926204-33926223	Msa0288570:CDS	30.0%
AATTTGGAGATAAAAGGGTT+TGG	-	chr2_3:33926245-33926264	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	33924667	33928473	33924667	ID=Msa0288570;Name=Msa0288570
chr2_3	mRNA	33924667	33928473	33924667	ID=Msa0288570-mRNA-1;Parent=Msa0288570;Name=Msa0288570-mRNA-1;_AED=0.06;_eAED=0.06;_QI=279\|0.66\|0.85\|0.85\|0.83\|0.85\|7\|335\|187
chr2_3	exon	33924667	33924921	33924667	ID=Msa0288570-mRNA-1:exon:11122;Parent=Msa0288570-mRNA-1
chr2_3	exon	33925010	33925088	33925010	ID=Msa0288570-mRNA-1:exon:11123;Parent=Msa0288570-mRNA-1
chr2_3	exon	33925189	33925278	33925189	ID=Msa0288570-mRNA-1:exon:11124;Parent=Msa0288570-mRNA-1
chr2_3	exon	33926132	33926226	33926132	ID=Msa0288570-mRNA-1:exon:11125;Parent=Msa0288570-mRNA-1
chr2_3	exon	33927012	33927080	33927012	ID=Msa0288570-mRNA-1:exon:11126;Parent=Msa0288570-mRNA-1
chr2_3	exon	33927467	33927559	33927467	ID=Msa0288570-mRNA-1:exon:11127;Parent=Msa0288570-mRNA-1
chr2_3	exon	33927977	33928473	33927977	ID=Msa0288570-mRNA-1:exon:11128;Parent=Msa0288570-mRNA-1
chr2_3	five_prime_UTR	33924667	33924921	33924667	ID=Msa0288570-mRNA-1:five_prime_utr;Parent=Msa0288570-mRNA-1
chr2_3	five_prime_UTR	33925010	33925033	33925010	ID=Msa0288570-mRNA-1:five_prime_utr;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33925034	33925088	33925034	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33925189	33925278	33925189	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33926132	33926226	33926132	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33927012	33927080	33927012	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33927467	33927559	33927467	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	CDS	33927977	33928138	33927977	ID=Msa0288570-mRNA-1:cds;Parent=Msa0288570-mRNA-1
chr2_3	three_prime_UTR	33928139	33928473	33928139	ID=Msa0288570-mRNA-1:three_prime_utr;Parent=Msa0288570-mRNA-1

Gene Sequence

>Msa0288570

ATGGAGCCTATGGATATTGTTGCTAAATCAAAGGAAGATGCTTCGCTACCTAAAGCGACGATGACGAAAATTATTAAAGAGATGTTGCCACCTGATGTCCGTGTTGCAAGAGATACCCAGGATTTGTTAATTGAATGTTGTGTAGAGTTCATAAACCTCGTCTCATCAGAGTCAAATGAAGTATGTAACAGAGAGGAAAGAAGGACGATTGCTCCTGAGCATGTATTGAAAGCTTTAGGGGTTCTTGGATTTGGCGAGTACATTGAGGAAGTTTATGCAGCATATGAACAGCACAAGATGGAGACAGTGTTTAGGTTCCCATATTGTAAGCAACTTGGCATAAATTATGTCCTGTGGGCAAGGCAACCTAAAGATACAGAGACCTCTCAGTGTGAGGTCAAGCAAGACTCTATAAAAGGTGCCAAGTGGAGCGGTGCAGCTGAGATGACCGAGGAACAAGCATTAGCAGAGCAGCAAAGGATGTTTGCAGAGGCACGTGCTAGAATGAATGGAGGGACCGTTACTTCCAATCAACCAGATGCTGACCAAAGTTTAGAGAACTAA

Protein sequence

>Msa0288570

MEPMDIVAKSKEDASLPKATMTKIIKEMLPPDVRVARDTQDLLIECCVEFINLVSSESNEVCNREERRTIAPEHVLKALGVLGFGEYIEEVYAAYEQHKMETVFRFPYCKQLGINYVLWARQPKDTETSQCEVKQDSIKGAKWSGAAEMTEEQALAEQQRMFAEARARMNGGTVTSNQPDADQSLEN*