AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0291360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0291360 MtrunA17_Chr3g0097771 75.610 41 10 0 45 85 475 515 6.82e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGGTCTTATCTTTGGTTCC+TGG 0.195860 2_3:+39857598 Msa0291360:three_prime_UTR
AACAACAACCTCAACGTATT+TGG 0.196067 2_3:-39856357 None:intergenic
TGATGCATTGCTTTGATTAT+TGG 0.229935 2_3:+39857790 Msa0291360:three_prime_UTR
CATCTGTCTGGTCTTATCTT+TGG 0.236205 2_3:+39857591 Msa0291360:three_prime_UTR
TGCCTTTACTGTAGTTTCTA+AGG 0.247816 2_3:+39857686 Msa0291360:three_prime_UTR
ATTTGATACAATTCTTTCTT+TGG 0.260065 2_3:-39857234 None:intergenic
CTTTCTTTGGTTGGTCCATA+AGG 0.322439 2_3:-39857221 None:intergenic
GCCTCTTAGTTAGCTTCCCT+TGG 0.330333 2_3:-39857351 None:intergenic
TTAGGTGTTGTGTGGAGTTA+TGG 0.330967 2_3:+39857661 Msa0291360:three_prime_UTR
TGGTCTTATCTTTGGTTCCT+GGG 0.349096 2_3:+39857599 Msa0291360:three_prime_UTR
TTTCTTTGGTTGGTCCATAA+GGG 0.358698 2_3:-39857220 None:intergenic
GGGGAATGGATGCACCCTTA+TGG 0.382705 2_3:+39857206 Msa0291360:CDS
CTTTAAAGCCTCATAAAATC+AGG 0.398749 2_3:-39857563 None:intergenic
AACGTCAGGAAGGTCGGTGG+TGG 0.404283 2_3:+39857401 Msa0291360:three_prime_UTR
GATACAATTCTTTCTTTGGT+TGG 0.409885 2_3:-39857230 None:intergenic
GCCTCAAAGAGGCGGCTTGA+TGG 0.417643 2_3:+39857299 Msa0291360:CDS
TTATGTTCCAGGGATATGTT+GGG 0.429619 2_3:+39857186 Msa0291360:intron
ACCATCAAGCCGCCTCTTTG+AGG 0.435602 2_3:-39857300 None:intergenic
TTGTCGTGTGCCAAGCAGTT+TGG 0.437649 2_3:+39857534 Msa0291360:three_prime_UTR
CCTCTTAGTTAGCTTCCCTT+GGG 0.449180 2_3:-39857350 None:intergenic
TAACATGAATCTTAAGTCTC+TGG 0.456122 2_3:+39856383 Msa0291360:CDS
TTTATGTTCCAGGGATATGT+TGG 0.462766 2_3:+39857185 Msa0291360:intron
TTTAAAGTGTCTCATCTGTC+TGG 0.466211 2_3:+39857579 Msa0291360:three_prime_UTR
AGTTAGCTTCCCTTGGGAGA+TGG 0.485070 2_3:-39857344 None:intergenic
AACGAAGGATCGTCAACGTC+AGG 0.492242 2_3:+39857387 Msa0291360:three_prime_UTR
CGTCAGGAAGGTCGGTGGTG+GGG 0.502493 2_3:+39857403 Msa0291360:three_prime_UTR
ACGTCAGGAAGGTCGGTGGT+GGG 0.503541 2_3:+39857402 Msa0291360:three_prime_UTR
GAAATGTGCAGGTGTGTTGG+TGG 0.511392 2_3:+39857446 Msa0291360:three_prime_UTR
AGGAAAGGCTTACAATGTAC+AGG 0.525754 2_3:-39857748 None:intergenic
AACAATATATTCGAAACTAC+AGG 0.526350 2_3:-39857768 None:intergenic
ATTGAAATGTGCAGGTGTGT+TGG 0.530564 2_3:+39857443 Msa0291360:three_prime_UTR
AGCCTTAGAAACTACAGTAA+AGG 0.542399 2_3:-39857688 None:intergenic
GTAAGTAGCACCGACAAGCT+TGG 0.553147 2_3:+39857257 Msa0291360:CDS
TCCAGGGATATGTTGGGGAA+TGG 0.553705 2_3:+39857192 Msa0291360:intron
CCCAAGGGAAGCTAACTAAG+AGG 0.562495 2_3:+39857350 Msa0291360:exon
AGATAACTGACCAAGCTTGT+CGG 0.571146 2_3:-39857267 None:intergenic
ATCGTCAACGTCAGGAAGGT+CGG 0.572972 2_3:+39857395 Msa0291360:three_prime_UTR
CGTATTTGGTGTATCGTCAC+CGG 0.574136 2_3:-39856343 None:intergenic
CGTCACCGGAAAATAAATAA+CGG 0.575795 2_3:-39856329 None:intergenic
TCGAAATCGTCCATCTCCCA+AGG 0.589418 2_3:+39857334 Msa0291360:CDS
TATATTCGAAACTACAGGAA+AGG 0.590569 2_3:-39857763 None:intergenic
TAACAGTCTAAAACAATCCC+AGG 0.609516 2_3:-39857616 None:intergenic
TATGTTCCAGGGATATGTTG+GGG 0.611382 2_3:+39857187 Msa0291360:intron
AAGGATCGTCAACGTCAGGA+AGG 0.612869 2_3:+39857391 Msa0291360:three_prime_UTR
TATCGAATGCCTCAAAGAGG+CGG 0.642616 2_3:+39857291 Msa0291360:CDS
CGAAATCGTCCATCTCCCAA+GGG 0.675696 2_3:+39857335 Msa0291360:CDS
CTGTATCGAATGCCTCAAAG+AGG 0.677618 2_3:+39857288 Msa0291360:CDS
GTCAACGTCAGGAAGGTCGG+TGG 0.679401 2_3:+39857398 Msa0291360:three_prime_UTR
ACGATACACCAAATACGTTG+AGG 0.680517 2_3:+39856349 Msa0291360:CDS
GCAGATTGTGTAGAAAACGA+AGG 0.705776 2_3:+39857372 Msa0291360:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTGTTATTAATACATAT+AGG + chr2_3:39856502-39856521 Msa0291360:intron 10.0%
!! ACTCATTTATTAATAAATTA+TGG + chr2_3:39856535-39856554 Msa0291360:intron 10.0%
!! TTTGTGAACAAAAAATATTA+TGG + chr2_3:39854400-39854419 Msa0291360:intron 15.0%
!! AGAAAATATAAAATCAAAAG+TGG - chr2_3:39854814-39854833 None:intergenic 15.0%
!!! TTAAGCTTATGTTTTTTTTA+AGG + chr2_3:39855255-39855274 Msa0291360:intron 15.0%
!!! TATGTTTTTTTTAAGGATTA+TGG + chr2_3:39855262-39855281 Msa0291360:intron 15.0%
!! AAATACATATGTAATTCAAA+CGG - chr2_3:39856047-39856066 None:intergenic 15.0%
!!! AGTTATTTATACTCATTTTA+AGG + chr2_3:39857155-39857174 Msa0291360:intron 15.0%
!!! ACTTACTTTAAAAGCTAATT+AGG + chr2_3:39854539-39854558 Msa0291360:intron 20.0%
!! TCAATTAATGCTAGTTAAAT+AGG - chr2_3:39854723-39854742 None:intergenic 20.0%
!! AACTAGCATTAATTGAAAAA+CGG + chr2_3:39854727-39854746 Msa0291360:intron 20.0%
!! TAATCAAATTACAACTTAGT+AGG - chr2_3:39855205-39855224 None:intergenic 20.0%
!!! TTGAGATTTTATTTTTCATC+AGG + chr2_3:39855332-39855351 Msa0291360:intron 20.0%
!!! TGAATTTTCGTTTTTTTCTA+TGG + chr2_3:39855491-39855510 Msa0291360:intron 20.0%
!!! ATTATTTTCACATAATGCAA+TGG + chr2_3:39855724-39855743 Msa0291360:intron 20.0%
!! GCAAATACATATAATTAGAT+TGG + chr2_3:39855850-39855869 Msa0291360:intron 20.0%
!!! TTTTATTTGTGTTCATGATT+TGG + chr2_3:39856098-39856117 Msa0291360:intron 20.0%
!! AACATAATCAACACTTTAAT+CGG - chr2_3:39856202-39856221 None:intergenic 20.0%
!! TATTAATAAATGAGTCACAT+TGG - chr2_3:39856530-39856549 None:intergenic 20.0%
!! TAATTATATAAGAGAGAATG+AGG + chr2_3:39856706-39856725 Msa0291360:intron 20.0%
!!! TAAACTTAATTTAAGATAGC+AGG - chr2_3:39857064-39857083 None:intergenic 20.0%
!! ATTTGATACAATTCTTTCTT+TGG - chr2_3:39857237-39857256 None:intergenic 20.0%
!!! AATGTTTTTTCTGTGTTTTT+AGG + chr2_3:39857643-39857662 Msa0291360:three_prime_UTR 20.0%
!!! TTTACTTTCGTAGATATTTT+TGG + chr2_3:39857710-39857729 Msa0291360:three_prime_UTR 20.0%
! ACAAAAAATATTATGGTTGC+CGG + chr2_3:39854407-39854426 Msa0291360:intron 25.0%
! TCAAACATTGAAGATATCAT+TGG + chr2_3:39854448-39854467 Msa0291360:intron 25.0%
! CTAACATGCATCATTTAATT+CGG + chr2_3:39854634-39854653 Msa0291360:intron 25.0%
! TTATAGTTCCAGTGTAAAAT+AGG + chr2_3:39854690-39854709 Msa0291360:intron 25.0%
! TATAGTTCCAGTGTAAAATA+GGG + chr2_3:39854691-39854710 Msa0291360:intron 25.0%
! GTTCTTTATAAGAGAAAGAA+TGG - chr2_3:39854904-39854923 None:intergenic 25.0%
!! TGGATGAAAAGTTTAATTTC+AGG - chr2_3:39854994-39855013 None:intergenic 25.0%
! CTTTGTTGAATATTTGTATC+AGG - chr2_3:39855057-39855076 None:intergenic 25.0%
! ATCTCAATCAAACTTAAACA+CGG - chr2_3:39855319-39855338 None:intergenic 25.0%
!! ATTTTTCATCAGGAAGAATA+AGG + chr2_3:39855342-39855361 Msa0291360:intron 25.0%
! AACATCAGAATATGCATTAT+TGG + chr2_3:39855762-39855781 Msa0291360:intron 25.0%
! ACATCAGAATATGCATTATT+GGG + chr2_3:39855763-39855782 Msa0291360:intron 25.0%
! ACTATGATTTCTAAATGACT+TGG + chr2_3:39855828-39855847 Msa0291360:intron 25.0%
!!! TTGTTTTTGTGTTCTGTTTT+TGG + chr2_3:39855895-39855914 Msa0291360:intron 25.0%
!!! TGTTTTTGTGTTCTGTTTTT+GGG + chr2_3:39855896-39855915 Msa0291360:intron 25.0%
!!! ATGTGTCGTTCATTTTTATT+TGG + chr2_3:39856129-39856148 Msa0291360:intron 25.0%
!!! GATTAAAGTGTTGATTATGT+TGG + chr2_3:39856201-39856220 Msa0291360:intron 25.0%
!!! TTTTTTCTCTACTTTCCATT+AGG + chr2_3:39856278-39856297 Msa0291360:intron 25.0%
!!! ACTTTCCGTTATTTATTTTC+CGG + chr2_3:39856324-39856343 Msa0291360:CDS 25.0%
!! ACAACAATGATTTTGAATAG+AGG - chr2_3:39856429-39856448 None:intergenic 25.0%
!!! ATTTTGAGTTTGTTGAGTTT+AGG + chr2_3:39856579-39856598 Msa0291360:intron 25.0%
! AACACAAAATGAGCATTATA+AGG - chr2_3:39856780-39856799 None:intergenic 25.0%
! AAACATCACTTATACTTCTT+TGG - chr2_3:39856807-39856826 None:intergenic 25.0%
!! TGTGTAGTTTTTAATGTCAA+AGG + chr2_3:39856844-39856863 Msa0291360:intron 25.0%
!!! TAATTTTGATTGAAATGTGC+AGG + chr2_3:39857435-39857454 Msa0291360:three_prime_UTR 25.0%
! AACAATATATTCGAAACTAC+AGG - chr2_3:39857771-39857790 None:intergenic 25.0%
AGAAGAAATAAACACATCGA+TGG + chr2_3:39854471-39854490 Msa0291360:intron 30.0%
GGAAATTAGCATAGCAATTA+GGG - chr2_3:39854581-39854600 None:intergenic 30.0%
ATAAAATCAAAAGTGGAGAC+TGG - chr2_3:39854807-39854826 None:intergenic 30.0%
TATGAAATGGAGAGAACAAA+AGG - chr2_3:39854840-39854859 None:intergenic 30.0%
ATGATATCTTGGTTGAAAGT+AGG - chr2_3:39854878-39854897 None:intergenic 30.0%
AAGAATGGAAGATGATATCT+TGG - chr2_3:39854889-39854908 None:intergenic 30.0%
GTTGAATATTTGTATCAGGA+TGG - chr2_3:39855053-39855072 None:intergenic 30.0%
!!! AAAAACTGGGTGAGTTTTTT+TGG + chr2_3:39855089-39855108 Msa0291360:intron 30.0%
! GTCATCATCAATTGTTTTCA+AGG + chr2_3:39855394-39855413 Msa0291360:intron 30.0%
TCAATGCGAAAAAAGTAACA+TGG - chr2_3:39855474-39855493 None:intergenic 30.0%
CAGACAGAACAAAAAAACAA+TGG - chr2_3:39855538-39855557 None:intergenic 30.0%
CCAAAGATATATATGTTGAG+AGG - chr2_3:39855599-39855618 None:intergenic 30.0%
CCTCTCAACATATATATCTT+TGG + chr2_3:39855596-39855615 Msa0291360:intron 30.0%
AAATCATAGTTACCTGAGTA+AGG - chr2_3:39855818-39855837 None:intergenic 30.0%
ATGACATAAAAACCCCAATT+CGG - chr2_3:39855925-39855944 None:intergenic 30.0%
TAACATGAATCTTAAGTCTC+TGG + chr2_3:39856383-39856402 Msa0291360:CDS 30.0%
AGAATGAGGATGTAAAGATA+CGG + chr2_3:39856720-39856739 Msa0291360:intron 30.0%
TGTGATGAAGTTAATCAAAG+AGG - chr2_3:39856969-39856988 None:intergenic 30.0%
GAAACTTAATTCAAGATAGC+AGG - chr2_3:39857112-39857131 None:intergenic 30.0%
!! AGGCTATATTTTTATGTTCC+AGG + chr2_3:39857175-39857194 Msa0291360:intron 30.0%
!! GGCTATATTTTTATGTTCCA+GGG + chr2_3:39857176-39857195 Msa0291360:intron 30.0%
GATACAATTCTTTCTTTGGT+TGG - chr2_3:39857233-39857252 None:intergenic 30.0%
!! CTTTAAAGCCTCATAAAATC+AGG - chr2_3:39857566-39857585 None:intergenic 30.0%
TATATTCGAAACTACAGGAA+AGG - chr2_3:39857766-39857785 None:intergenic 30.0%
!! ATTTGCTATCCTTCAATCAC+CGG - chr2_3:39854429-39854448 None:intergenic 35.0%
GGGAAATTAGCATAGCAATT+AGG - chr2_3:39854582-39854601 None:intergenic 35.0%
TAATTTCCCTCCTAATCACA+TGG + chr2_3:39854593-39854612 Msa0291360:intron 35.0%
!! GTTAGCTTGTTGTGTAAGAT+TGG - chr2_3:39854619-39854638 None:intergenic 35.0%
TTACACTGGAACTATAACCT+AGG - chr2_3:39854687-39854706 None:intergenic 35.0%
TATCTTGGTTGAAAGTAGGA+GGG - chr2_3:39854874-39854893 None:intergenic 35.0%
ATATCTTGGTTGAAAGTAGG+AGG - chr2_3:39854875-39854894 None:intergenic 35.0%
TTGGAGTTGAACTAAGGATT+TGG - chr2_3:39854926-39854945 None:intergenic 35.0%
! AATCTCGCGTCTTGTTAAAT+CGG - chr2_3:39854955-39854974 None:intergenic 35.0%
GGATGAACTGATGAACAAAT+TGG - chr2_3:39855014-39855033 None:intergenic 35.0%
TATTTGTATCAGGATGGTGT+TGG - chr2_3:39855047-39855066 None:intergenic 35.0%
AGTGTCCAACATTCAAAAAC+TGG + chr2_3:39855075-39855094 Msa0291360:intron 35.0%
GTGTCCAACATTCAAAAACT+GGG + chr2_3:39855076-39855095 Msa0291360:intron 35.0%
AGGAGAATCTCAAAAACGAA+AGG - chr2_3:39855139-39855158 None:intergenic 35.0%
GCAAACAGGTTATTAAGATC+AGG - chr2_3:39855159-39855178 None:intergenic 35.0%
!! TTGATGATGACTCATAATCC+TGG - chr2_3:39855385-39855404 None:intergenic 35.0%
!! TGTTTTCAAGGTTTGAGACA+AGG + chr2_3:39855406-39855425 Msa0291360:intron 35.0%
!! GTTTTCAAGGTTTGAGACAA+GGG + chr2_3:39855407-39855426 Msa0291360:intron 35.0%
!!! TGTTGGAGTGTTTTGATTGT+TGG + chr2_3:39856218-39856237 Msa0291360:intron 35.0%
!!! GTTGGAGTGTTTTGATTGTT+GGG + chr2_3:39856219-39856238 Msa0291360:intron 35.0%
TTGTTGGGATTTAGATTGAG+AGG + chr2_3:39856234-39856253 Msa0291360:intron 35.0%
CGTTGTCGAAAAATACCTAA+TGG - chr2_3:39856296-39856315 None:intergenic 35.0%
CGTCACCGGAAAATAAATAA+CGG - chr2_3:39856332-39856351 None:intergenic 35.0%
AACAACAACCTCAACGTATT+TGG - chr2_3:39856360-39856379 None:intergenic 35.0%
CAAGCTATACTAAGAGTAAG+AGG - chr2_3:39856932-39856951 None:intergenic 35.0%
TGACCTTCAAGCAAAGTAAT+GGG + chr2_3:39857023-39857042 Msa0291360:intron 35.0%
TTTATGTTCCAGGGATATGT+TGG + chr2_3:39857185-39857204 Msa0291360:intron 35.0%
TTATGTTCCAGGGATATGTT+GGG + chr2_3:39857186-39857205 Msa0291360:intron 35.0%
! TTTCTTTGGTTGGTCCATAA+GGG - chr2_3:39857223-39857242 None:intergenic 35.0%
! GGAAAAAGCCTGATTTTATG+AGG + chr2_3:39857555-39857574 Msa0291360:three_prime_UTR 35.0%
! TTTAAAGTGTCTCATCTGTC+TGG + chr2_3:39857579-39857598 Msa0291360:three_prime_UTR 35.0%
! TAACAGTCTAAAACAATCCC+AGG - chr2_3:39857619-39857638 None:intergenic 35.0%
TGCCTTTACTGTAGTTTCTA+AGG + chr2_3:39857686-39857705 Msa0291360:three_prime_UTR 35.0%
AGCCTTAGAAACTACAGTAA+AGG - chr2_3:39857691-39857710 None:intergenic 35.0%
! GTAAGATTGGCCATGTGATT+AGG - chr2_3:39854606-39854625 None:intergenic 40.0%
GGAGAAAGATGCGTATGAAA+TGG - chr2_3:39854853-39854872 None:intergenic 40.0%
TGGTTGTTGGAGTTGAACTA+AGG - chr2_3:39854932-39854951 None:intergenic 40.0%
CTTGTTAAATCGGTGGTTGT+TGG - chr2_3:39854945-39854964 None:intergenic 40.0%
! CTCACCCAGTTTTTGAATGT+TGG - chr2_3:39855083-39855102 None:intergenic 40.0%
CTACAAAGGAGAAAGCAAAC+AGG - chr2_3:39855173-39855192 None:intergenic 40.0%
GTAGGATGACAGTACTACAA+AGG - chr2_3:39855187-39855206 None:intergenic 40.0%
GCTTTCACATTCATGATTCC+AGG + chr2_3:39855364-39855383 Msa0291360:intron 40.0%
! AAGGTTTGAGACAAGGGAAT+TGG + chr2_3:39855413-39855432 Msa0291360:intron 40.0%
!! TGTTCTGTCTGTAGCTTTAG+TGG + chr2_3:39855547-39855566 Msa0291360:intron 40.0%
!! CATCTAGAAGCATTGTGTCA+AGG - chr2_3:39855624-39855643 None:intergenic 40.0%
!!! TGTTTTTGGGTAGCCGAATT+GGG + chr2_3:39855909-39855928 Msa0291360:intron 40.0%
AAGTTGACGTTCGCATAGAT+GGG - chr2_3:39856260-39856279 None:intergenic 40.0%
AAAGTTGACGTTCGCATAGA+TGG - chr2_3:39856261-39856280 None:intergenic 40.0%
ACGATACACCAAATACGTTG+AGG + chr2_3:39856349-39856368 Msa0291360:CDS 40.0%
CTGACCTTCAAGCAAAGTAA+TGG + chr2_3:39857022-39857041 Msa0291360:intron 40.0%
AGTCCCATTACTTTGCTTGA+AGG - chr2_3:39857029-39857048 None:intergenic 40.0%
TAAGATAGCAGGACAATGTC+TGG - chr2_3:39857053-39857072 None:intergenic 40.0%
TATGTTCCAGGGATATGTTG+GGG + chr2_3:39857187-39857206 Msa0291360:intron 40.0%
! CTTTCTTTGGTTGGTCCATA+AGG - chr2_3:39857224-39857243 None:intergenic 40.0%
AGATAACTGACCAAGCTTGT+CGG - chr2_3:39857270-39857289 None:intergenic 40.0%
GCAGATTGTGTAGAAAACGA+AGG + chr2_3:39857372-39857391 Msa0291360:three_prime_UTR 40.0%
ATTGAAATGTGCAGGTGTGT+TGG + chr2_3:39857443-39857462 Msa0291360:three_prime_UTR 40.0%
!! CATCTGTCTGGTCTTATCTT+TGG + chr2_3:39857591-39857610 Msa0291360:three_prime_UTR 40.0%
!! TGGTCTTATCTTTGGTTCCT+GGG + chr2_3:39857599-39857618 Msa0291360:three_prime_UTR 40.0%
!!! CTGTGTTTTTAGGTGTTGTG+TGG + chr2_3:39857653-39857672 Msa0291360:three_prime_UTR 40.0%
! TTAGGTGTTGTGTGGAGTTA+TGG + chr2_3:39857661-39857680 Msa0291360:three_prime_UTR 40.0%
AGGAAAGGCTTACAATGTAC+AGG - chr2_3:39857751-39857770 None:intergenic 40.0%
TTATGGTTGCCGGTGATTGA+AGG + chr2_3:39854417-39854436 Msa0291360:intron 45.0%
! GATTGGCCATGTGATTAGGA+GGG - chr2_3:39854602-39854621 None:intergenic 45.0%
! AGATTGGCCATGTGATTAGG+AGG - chr2_3:39854603-39854622 None:intergenic 45.0%
AACCTAGGTCGCTGTATTCT+AGG - chr2_3:39854672-39854691 None:intergenic 45.0%
TCCAGTGTAAAATAGGGCAG+CGG + chr2_3:39854697-39854716 Msa0291360:intron 45.0%
! ATGAAGTGTGATGGTGCACA+GGG + chr2_3:39854757-39854776 Msa0291360:intron 45.0%
TTAACAAGACGCGAGATTGC+TGG + chr2_3:39854957-39854976 Msa0291360:intron 45.0%
TAACAAGACGCGAGATTGCT+GGG + chr2_3:39854958-39854977 Msa0291360:intron 45.0%
! ATCAGGATGGTGTTGGAAGT+TGG - chr2_3:39855040-39855059 None:intergenic 45.0%
TGAGACAAGGGAATTGGTGT+TGG + chr2_3:39855419-39855438 Msa0291360:intron 45.0%
TATTGGGAAAGAGCTTGCAG+TGG + chr2_3:39855779-39855798 Msa0291360:intron 45.0%
! ATGGTGATGTCTCCTTACTC+AGG + chr2_3:39855803-39855822 Msa0291360:intron 45.0%
!!! CTGTTTTTGGGTAGCCGAAT+TGG + chr2_3:39855908-39855927 Msa0291360:intron 45.0%
!!! GTTTTTGGGTAGCCGAATTG+GGG + chr2_3:39855910-39855929 Msa0291360:intron 45.0%
!! CGTATTTGGTGTATCGTCAC+CGG - chr2_3:39856346-39856365 None:intergenic 45.0%
CTGTATCGAATGCCTCAAAG+AGG + chr2_3:39857288-39857307 Msa0291360:CDS 45.0%
TATCGAATGCCTCAAAGAGG+CGG + chr2_3:39857291-39857310 Msa0291360:CDS 45.0%
! CCTCTTAGTTAGCTTCCCTT+GGG - chr2_3:39857353-39857372 None:intergenic 45.0%
!! CAGGCTTTTTCCAAACTGCT+TGG - chr2_3:39857547-39857566 None:intergenic 45.0%
!! CTGGTCTTATCTTTGGTTCC+TGG + chr2_3:39857598-39857617 Msa0291360:three_prime_UTR 45.0%
GTGCACCATCACACTTCATG+CGG - chr2_3:39854756-39854775 None:intergenic 50.0%
! CATGAAGTGTGATGGTGCAC+AGG + chr2_3:39854756-39854775 Msa0291360:intron 50.0%
! CTCGCGTCTTGTTAAATCGG+TGG - chr2_3:39854952-39854971 None:intergenic 50.0%
! GATGGTGTTGGAAGTTGGAC+TGG - chr2_3:39855035-39855054 None:intergenic 50.0%
GGAAAGAGCTTGCAGTGGTA+TGG + chr2_3:39855784-39855803 Msa0291360:intron 50.0%
TCCAGGGATATGTTGGGGAA+TGG + chr2_3:39857192-39857211 Msa0291360:intron 50.0%
TCCATTCCCCAACATATCCC+TGG - chr2_3:39857196-39857215 None:intergenic 50.0%
!! GTAAGTAGCACCGACAAGCT+TGG + chr2_3:39857257-39857276 Msa0291360:CDS 50.0%
TCGAAATCGTCCATCTCCCA+AGG + chr2_3:39857334-39857353 Msa0291360:CDS 50.0%
CGAAATCGTCCATCTCCCAA+GGG + chr2_3:39857335-39857354 Msa0291360:CDS 50.0%
! AGTTAGCTTCCCTTGGGAGA+TGG - chr2_3:39857347-39857366 None:intergenic 50.0%
CCCAAGGGAAGCTAACTAAG+AGG + chr2_3:39857350-39857369 Msa0291360:exon 50.0%
! GCCTCTTAGTTAGCTTCCCT+TGG - chr2_3:39857354-39857373 None:intergenic 50.0%
AACGAAGGATCGTCAACGTC+AGG + chr2_3:39857387-39857406 Msa0291360:three_prime_UTR 50.0%
AAGGATCGTCAACGTCAGGA+AGG + chr2_3:39857391-39857410 Msa0291360:three_prime_UTR 50.0%
ATCGTCAACGTCAGGAAGGT+CGG + chr2_3:39857395-39857414 Msa0291360:three_prime_UTR 50.0%
GAAATGTGCAGGTGTGTTGG+TGG + chr2_3:39857446-39857465 Msa0291360:three_prime_UTR 50.0%
TTGTCGTGTGCCAAGCAGTT+TGG + chr2_3:39857534-39857553 Msa0291360:three_prime_UTR 50.0%
CGCCTAGAATACAGCGACCT+AGG + chr2_3:39854667-39854686 Msa0291360:intron 55.0%
! GCCGCTGCCCTATTTTACAC+TGG - chr2_3:39854701-39854720 None:intergenic 55.0%
GGTGACCGCATGAAGTGTGA+TGG + chr2_3:39854748-39854767 Msa0291360:intron 55.0%
GGTGCACAGGGCTCAACAAT+TGG + chr2_3:39854769-39854788 Msa0291360:intron 55.0%
GGGGAATGGATGCACCCTTA+TGG + chr2_3:39857206-39857225 Msa0291360:CDS 55.0%
ACCATCAAGCCGCCTCTTTG+AGG - chr2_3:39857303-39857322 None:intergenic 55.0%
GCCTCAAAGAGGCGGCTTGA+TGG + chr2_3:39857299-39857318 Msa0291360:CDS 60.0%
GTCAACGTCAGGAAGGTCGG+TGG + chr2_3:39857398-39857417 Msa0291360:three_prime_UTR 60.0%
AACGTCAGGAAGGTCGGTGG+TGG + chr2_3:39857401-39857420 Msa0291360:three_prime_UTR 60.0%
ACGTCAGGAAGGTCGGTGGT+GGG + chr2_3:39857402-39857421 Msa0291360:three_prime_UTR 60.0%
CGTCAGGAAGGTCGGTGGTG+GGG + chr2_3:39857403-39857422 Msa0291360:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 39854371 39857811 39854371 ID=Msa0291360;Name=Msa0291360
chr2_3 mRNA 39854371 39857811 39854371 ID=Msa0291360-mRNA-1;Parent=Msa0291360;Name=Msa0291360-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0|0.2|0.4|1|1|5|447|98
chr2_3 exon 39854371 39854378 39854371 ID=Msa0291360-mRNA-1:exon:12820;Parent=Msa0291360-mRNA-1
chr2_3 exon 39855234 39855240 39855234 ID=Msa0291360-mRNA-1:exon:12821;Parent=Msa0291360-mRNA-1
chr2_3 exon 39855882 39855890 39855882 ID=Msa0291360-mRNA-1:exon:12822;Parent=Msa0291360-mRNA-1
chr2_3 exon 39856300 39856404 39856300 ID=Msa0291360-mRNA-1:exon:12823;Parent=Msa0291360-mRNA-1
chr2_3 exon 39857197 39857811 39857197 ID=Msa0291360-mRNA-1:exon:12824;Parent=Msa0291360-mRNA-1
chr2_3 CDS 39854371 39854378 39854371 ID=Msa0291360-mRNA-1:cds;Parent=Msa0291360-mRNA-1
chr2_3 CDS 39855234 39855240 39855234 ID=Msa0291360-mRNA-1:cds;Parent=Msa0291360-mRNA-1
chr2_3 CDS 39855882 39855890 39855882 ID=Msa0291360-mRNA-1:cds;Parent=Msa0291360-mRNA-1
chr2_3 CDS 39856300 39856404 39856300 ID=Msa0291360-mRNA-1:cds;Parent=Msa0291360-mRNA-1
chr2_3 CDS 39857197 39857364 39857197 ID=Msa0291360-mRNA-1:cds;Parent=Msa0291360-mRNA-1
chr2_3 three_prime_UTR 39857365 39857811 39857365 ID=Msa0291360-mRNA-1:three_prime_utr;Parent=Msa0291360-mRNA-1
Gene Sequence

>Msa0291360

ATGGAGATATTCTGCAAGAGCGATGTATTTTTCGACAACGATACAACTACTTTCCGTTATTTATTTTCCGGTGACGATACACCAAATACGTTGAGGTTGTTGTTAAGTAACATGAATCTTAAGTCTCTGGGATATGTTGGGGAATGGATGCACCCTTATGGACCAACCAAAGAAAGAATTGTATCAAATGTAAGTAGCACCGACAAGCTTGGTCAGTTATCTGTATCGAATGCCTCAAAGAGGCGGCTTGATGGTTATGAAAGATGTCGAAATCGTCCATCTCCCAAGGGAAGCTAA
Protein sequence

>Msa0291360

MEIFCKSDVFFDNDTTTFRYLFSGDDTPNTLRLLLSNMNLKSLGYVGEWMHPYGPTKERIVSNVSSTDKLGQLSVSNASKRRLDGYERCRNRPSPKGS*