Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293660 | XP_003593026.2 | 91.045 | 67 | 6 | 0 | 1 | 67 | 749 | 815 | 7.22e-33 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293660 | sp|Q0WL80|UGGG_ARATH | 50.000 | 62 | 31 | 0 | 5 | 66 | 728 | 789 | 4.04e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293660 | G7IPG7 | 91.045 | 67 | 6 | 0 | 1 | 67 | 749 | 815 | 3.45e-33 | 132 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0293660 | Msa1184910 | 0.801208 | 1.003323e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293660 | MtrunA17_Chr2g0277801 | 91.045 | 67 | 6 | 0 | 1 | 67 | 749 | 815 | 6.65e-37 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0293660 | AT1G71220.1 | 50.000 | 62 | 31 | 0 | 5 | 66 | 728 | 789 | 4.11e-15 | 70.1 |
| Msa0293660 | AT1G71220.2 | 50.000 | 62 | 31 | 0 | 5 | 66 | 728 | 789 | 4.11e-15 | 70.1 |
| Msa0293660 | AT1G71220.3 | 50.000 | 62 | 31 | 0 | 5 | 66 | 766 | 827 | 4.11e-15 | 70.1 |
Find 20 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGTTGGGCCTACTTATTC+AGG | 0.264652 | 2_3:+45100849 | Msa0293660:CDS |
| TCTCTTTCGATGTTTACTTT+TGG | 0.293916 | 2_3:+45100520 | Msa0293660:CDS |
| CCGCCAAGGCATAAATTATC+TGG | 0.296609 | 2_3:+45100758 | Msa0293660:CDS |
| ATGACAGGCACCAGCCTAAA+AGG | 0.303296 | 2_3:-45100819 | None:intergenic |
| CTTGCTGTTGACATCACTTC+AGG | 0.333188 | 2_3:+45100717 | Msa0293660:CDS |
| TAACCAGATAATTTATGCCT+TGG | 0.412765 | 2_3:-45100761 | None:intergenic |
| ATCGAAAGAGATATGAACTT+TGG | 0.420496 | 2_3:-45100508 | None:intergenic |
| CCAGATAATTTATGCCTTGG+CGG | 0.427716 | 2_3:-45100758 | None:intergenic |
| CTGTCATGAGTTGGAGTGTT+GGG | 0.430259 | 2_3:+45100835 | Msa0293660:CDS |
| ATTAACTACTTACACTCGTC+TGG | 0.498278 | 2_3:+45100565 | Msa0293660:CDS |
| GGCTGGTGCCTGTCATGAGT+TGG | 0.499572 | 2_3:+45100826 | Msa0293660:CDS |
| GTTGACATCACTTCAGGAAG+TGG | 0.544599 | 2_3:+45100723 | Msa0293660:CDS |
| AATAGCAGTCCTGAATAAGT+AGG | 0.551214 | 2_3:-45100858 | None:intergenic |
| CCTGTCATGAGTTGGAGTGT+TGG | 0.558616 | 2_3:+45100834 | Msa0293660:CDS |
| TTGACATCACTTCAGGAAGT+GGG | 0.571258 | 2_3:+45100724 | Msa0293660:CDS |
| CACAGGCTTCAAATCGTCCA+TGG | 0.575917 | 2_3:-45100685 | None:intergenic |
| CCAACACTCCAACTCATGAC+AGG | 0.581744 | 2_3:-45100834 | None:intergenic |
| ACAGCAAGAAGATGTGTCAC+AGG | 0.581776 | 2_3:-45100702 | None:intergenic |
| GGGATTAAGTTATTCCGCCA+AGG | 0.601342 | 2_3:+45100744 | Msa0293660:CDS |
| ACGAGTGTAAGTAGTTAATG+CGG | 0.691845 | 2_3:-45100561 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGATATGTTAGAATAAAT+AGG | + | chr2_3:45100597-45100616 | Msa0293660:intron | 20.0% |
| !!! | TGAATATTATTCTTCCTTTT+AGG | + | chr2_3:45100805-45100824 | Msa0293660:CDS | 20.0% |
| ATCGAAAGAGATATGAACTT+TGG | - | chr2_3:45100511-45100530 | None:intergenic | 30.0% | |
| ! | TCTCTTTCGATGTTTACTTT+TGG | + | chr2_3:45100520-45100539 | Msa0293660:CDS | 30.0% |
| ! | TGGTCTTTTGTCTTATCATT+TGG | + | chr2_3:45100642-45100661 | Msa0293660:intron | 30.0% |
| TAACCAGATAATTTATGCCT+TGG | - | chr2_3:45100764-45100783 | None:intergenic | 30.0% | |
| ! | TATTATTCTTCCTTTTAGGC+TGG | + | chr2_3:45100809-45100828 | Msa0293660:CDS | 30.0% |
| !! | TTCGATGTTTACTTTTGGAG+AGG | + | chr2_3:45100525-45100544 | Msa0293660:CDS | 35.0% |
| ACGAGTGTAAGTAGTTAATG+CGG | - | chr2_3:45100564-45100583 | None:intergenic | 35.0% | |
| ATTAACTACTTACACTCGTC+TGG | + | chr2_3:45100565-45100584 | Msa0293660:CDS | 35.0% | |
| ! | GTTTTTATCCAATACAGCCA+TGG | + | chr2_3:45100668-45100687 | Msa0293660:intron | 35.0% |
| AATAGCAGTCCTGAATAAGT+AGG | - | chr2_3:45100861-45100880 | None:intergenic | 35.0% | |
| CTTTCCTAATTCCTACACAC+TGG | + | chr2_3:45100622-45100641 | Msa0293660:intron | 40.0% | |
| AAGACCAGTGTGTAGGAATT+AGG | - | chr2_3:45100629-45100648 | None:intergenic | 40.0% | |
| AAGACAAAAGACCAGTGTGT+AGG | - | chr2_3:45100636-45100655 | None:intergenic | 40.0% | |
| TTGACATCACTTCAGGAAGT+GGG | + | chr2_3:45100724-45100743 | Msa0293660:CDS | 40.0% | |
| CCAGATAATTTATGCCTTGG+CGG | - | chr2_3:45100761-45100780 | None:intergenic | 40.0% | |
| CAAATCGTCCATGGCTGTAT+TGG | - | chr2_3:45100679-45100698 | None:intergenic | 45.0% | |
| ACAGCAAGAAGATGTGTCAC+AGG | - | chr2_3:45100705-45100724 | None:intergenic | 45.0% | |
| !! | CTTGCTGTTGACATCACTTC+AGG | + | chr2_3:45100717-45100736 | Msa0293660:CDS | 45.0% |
| GTTGACATCACTTCAGGAAG+TGG | + | chr2_3:45100723-45100742 | Msa0293660:CDS | 45.0% | |
| GGGATTAAGTTATTCCGCCA+AGG | + | chr2_3:45100744-45100763 | Msa0293660:CDS | 45.0% | |
| CCGCCAAGGCATAAATTATC+TGG | + | chr2_3:45100758-45100777 | Msa0293660:CDS | 45.0% | |
| ! | CTGTCATGAGTTGGAGTGTT+GGG | + | chr2_3:45100835-45100854 | Msa0293660:CDS | 45.0% |
| ! | AGTGTTGGGCCTACTTATTC+AGG | + | chr2_3:45100849-45100868 | Msa0293660:CDS | 45.0% |
| CACAGGCTTCAAATCGTCCA+TGG | - | chr2_3:45100688-45100707 | None:intergenic | 50.0% | |
| ATGACAGGCACCAGCCTAAA+AGG | - | chr2_3:45100822-45100841 | None:intergenic | 50.0% | |
| CCAACACTCCAACTCATGAC+AGG | - | chr2_3:45100837-45100856 | None:intergenic | 50.0% | |
| ! | CCTGTCATGAGTTGGAGTGT+TGG | + | chr2_3:45100834-45100853 | Msa0293660:CDS | 50.0% |
| GGCTGGTGCCTGTCATGAGT+TGG | + | chr2_3:45100826-45100845 | Msa0293660:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 45100490 | 45100961 | 45100490 | ID=Msa0293660;Name=Msa0293660 |
| chr2_3 | mRNA | 45100490 | 45100961 | 45100490 | ID=Msa0293660-mRNA-1;Parent=Msa0293660;Name=Msa0293660-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.5|1|1|1|2|41|111 |
| chr2_3 | exon | 45100490 | 45100589 | 45100490 | ID=Msa0293660-mRNA-1:exon:14047;Parent=Msa0293660-mRNA-1 |
| chr2_3 | exon | 45100685 | 45100961 | 45100685 | ID=Msa0293660-mRNA-1:exon:14048;Parent=Msa0293660-mRNA-1 |
| chr2_3 | CDS | 45100490 | 45100589 | 45100490 | ID=Msa0293660-mRNA-1:cds;Parent=Msa0293660-mRNA-1 |
| chr2_3 | CDS | 45100685 | 45100920 | 45100685 | ID=Msa0293660-mRNA-1:cds;Parent=Msa0293660-mRNA-1 |
| chr2_3 | three_prime_UTR | 45100921 | 45100961 | 45100921 | ID=Msa0293660-mRNA-1:three_prime_utr;Parent=Msa0293660-mRNA-1 |
| Gene Sequence |
| Protein sequence |
