AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0295560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295560 MtrunA17_Chr3g0077371 97.970 197 4 0 1 197 1 197 2.62e-143 397
Msa0295560 MtrunA17_Chr3g0077391 97.970 197 4 0 1 197 1 197 2.62e-143 397
Msa0295560 MtrunA17_Chr4g0009481 97.970 197 4 0 1 197 1 197 2.62e-143 397
Msa0295560 MtrunA17_Chr7g0241891 96.954 197 6 0 1 197 1 197 1.19e-141 392
Msa0295560 MtrunA17_Chr1g0182531 96.447 197 7 0 1 197 28 224 2.25e-140 390
Msa0295560 MtrunA17_Chr4g0060551 94.783 115 6 0 1 115 1 115 1.75e-76 224
Msa0295560 MtrunA17_Chr4g0009501 100.000 60 0 0 138 197 13 72 7.59e-38 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295560 AT5G39850.1 89.305 187 20 0 1 187 1 187 2.69e-122 344
Msa0295560 AT5G15200.1 82.828 198 33 1 1 197 1 198 1.76e-115 327
Msa0295560 AT5G15200.2 86.861 137 18 0 1 137 1 137 3.75e-84 246

Find 76 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCATAGTTTCGGTAAAA+TGG 0.192500 2_3:-48171567 None:intergenic
CCCTTTGGTGGTGGTCTTCC+TGG 0.209962 2_3:+48172558 Msa0295560:CDS
CGCTGTTTGCCTTGCCTACT+AGG 0.228229 2_3:-48171512 None:intergenic
GAACATCAAAGCAAAATATT+TGG 0.247831 2_3:-48172936 None:intergenic
ATCACCACCAGAAGCCTTCT+TGG 0.255696 2_3:-48172613 None:intergenic
AGTGAACATCCCATCCTTTA+TGG 0.285235 2_3:+48172491 Msa0295560:CDS
CAGACTCTTGTCTTCAAATC+TGG 0.288805 2_3:+48171888 Msa0295560:CDS
TTGAAGCTGGTCGGAGAGTA+TGG 0.314070 2_3:+48171642 Msa0295560:CDS
CCTTCTTGGCTGCAGCCTTA+AGG 0.354248 2_3:-48172599 None:intergenic
TTTGCTTTGATGTTCTCTTC+AGG 0.360413 2_3:+48172944 Msa0295560:three_prime_UTR
ACCATGCTAGGGTTCTTATC+AGG 0.369101 2_3:+48171928 Msa0295560:CDS
TGGACGACGGGGCTTCTTGA+AGG 0.391435 2_3:-48171592 None:intergenic
TGAATCAACCCTCACCATAA+AGG 0.402973 2_3:-48172505 None:intergenic
CTCCTTCTCGTATGGACGAC+GGG 0.404289 2_3:-48171604 None:intergenic
GCTGCAGCCAAGAAGGCTTC+TGG 0.408125 2_3:+48172606 Msa0295560:CDS
TTGTCTAGACATAAATTAGA+AGG 0.418678 2_3:-48172810 None:intergenic
TCCATACGAGAAGGAGCGTT+TGG 0.432310 2_3:+48171611 Msa0295560:CDS
TCTTCTCATCCAATGTCAAA+AGG 0.436967 2_3:-48171734 None:intergenic
TTATCAGGCAGAGACACATT+AGG 0.445935 2_3:+48171943 Msa0295560:CDS
CCTAGCATGGTGAATGGACT+TGG 0.448468 2_3:-48171916 None:intergenic
AATTAACTCACATATTCTCT+TGG 0.449883 2_3:+48172662 Msa0295560:three_prime_UTR
TTCTTTCGCTTCACGCGACC+AGG 0.452567 2_3:-48172576 None:intergenic
TTCTAGCATTGTTACGGATA+CGG 0.467109 2_3:-48171710 None:intergenic
ACCAGGAAGACCACCACCAA+AGG 0.473047 2_3:-48172559 None:intergenic
AAAGGGTTCTAGCATTGTTA+CGG 0.475438 2_3:-48171716 None:intergenic
TCCCTCACTAGTCCCTTTGG+TGG 0.477112 2_3:+48172546 Msa0295560:CDS
CTCACTAGTCCCTTTGGTGG+TGG 0.477263 2_3:+48172549 Msa0295560:CDS
GAACCCTCGTCGGATCTTTG+AGG 0.484508 2_3:+48171755 Msa0295560:CDS
AAGAAGGCTTCTGGTGGTGA+TGG 0.495137 2_3:+48172615 Msa0295560:CDS
AACCCTCGTCGGATCTTTGA+GGG 0.508041 2_3:+48171756 Msa0295560:CDS
CTTCTCATCCAATGTCAAAA+GGG 0.512873 2_3:-48171733 None:intergenic
CCTTAAGGCTGCAGCCAAGA+AGG 0.517095 2_3:+48172599 Msa0295560:CDS
TCCAAACGCTCCTTCTCGTA+TGG 0.519540 2_3:-48171612 None:intergenic
CCAAGTCCATTCACCATGCT+AGG 0.525789 2_3:+48171916 Msa0295560:CDS
TACTGGTCGCTTGAAGTGAA+AGG 0.535254 2_3:+48172694 Msa0295560:three_prime_UTR
AAGAACCCTAGCATGGTGAA+TGG 0.539163 2_3:-48171922 None:intergenic
GCTGCTGCTTAGAAGGCATC+CGG 0.541095 2_3:+48172726 Msa0295560:intron
CGTGAAGCGAAAGAACCTTA+AGG 0.541128 2_3:+48172584 Msa0295560:CDS
TGGTCGGAGAGTATGGTCTT+CGG 0.541482 2_3:+48171649 Msa0295560:CDS
CATCCCATCCTTTATGGTGA+GGG 0.542521 2_3:+48172497 Msa0295560:CDS
GTTTCGGTAAAATGGAATGT+GGG 0.544915 2_3:-48171559 None:intergenic
TCAACCCTCACCATAAAGGA+TGG 0.551266 2_3:-48172501 None:intergenic
CTAGACATAAATTAGAAGGC+AGG 0.556919 2_3:-48172806 None:intergenic
TTGAGGAGAATGTTTAAGCA+TGG 0.560766 2_3:+48171789 Msa0295560:CDS
GAACAAGCTCGATTATGTGT+TGG 0.578338 2_3:+48171830 Msa0295560:CDS
TTTGGATGCTGAGTTGAAGC+TGG 0.579570 2_3:+48171629 Msa0295560:CDS
CCAGGAAGACCACCACCAAA+GGG 0.581024 2_3:-48172558 None:intergenic
ATCCGGTGGTGACGAAAACA+AGG 0.583864 2_3:+48172743 Msa0295560:three_prime_UTR
GGTGTAAGAGAGAGCTGTGG+AGG 0.589621 2_3:+48171670 Msa0295560:CDS
TTCTGGTGGTGATGGAGATG+AGG 0.590267 2_3:+48172623 Msa0295560:CDS
AGTTTCGGTAAAATGGAATG+TGG 0.592077 2_3:-48171560 None:intergenic
TCTTGTCTTCAAATCTGGCA+TGG 0.593384 2_3:+48171893 Msa0295560:CDS
CAAGTCCATTCACCATGCTA+GGG 0.599707 2_3:+48171917 Msa0295560:CDS
GCAGCCAAGAAGGCTTCTGG+TGG 0.599775 2_3:+48172609 Msa0295560:CDS
ACATCCCATCCTTTATGGTG+AGG 0.599966 2_3:+48172496 Msa0295560:CDS
CAACCCTCACCATAAAGGAT+GGG 0.612413 2_3:-48172500 None:intergenic
ACCACCAAAGGGACTAGTGA+GGG 0.615882 2_3:-48172547 None:intergenic
GCCCCGTCGTCCATACGAGA+AGG 0.617765 2_3:+48171602 Msa0295560:CDS
GCTGCTTAGAAGGCATCCGG+TGG 0.622553 2_3:+48172729 Msa0295560:intron
GTAGGCAAGGCAAACAGCGG+CGG 0.627596 2_3:+48171516 Msa0295560:five_prime_UTR
TGAAGACAAGAGTCTGAAGA+CGG 0.630423 2_3:-48171881 None:intergenic
TTTCGGTAAAATGGAATGTG+GGG 0.638887 2_3:-48171558 None:intergenic
GATGCTGAGTTGAAGCTGGT+CGG 0.640820 2_3:+48171633 Msa0295560:CDS
GCCTGATAAGAACCCTAGCA+TGG 0.650504 2_3:-48171929 None:intergenic
GCTCCTTCTCGTATGGACGA+CGG 0.657592 2_3:-48171605 None:intergenic
TGGTGGTGATGGAGATGAGG+AGG 0.657794 2_3:+48172626 Msa0295560:CDS
GTGTAAGAGAGAGCTGTGGA+GGG 0.669438 2_3:+48171671 Msa0295560:CDS
CACCACCAAAGGGACTAGTG+AGG 0.678453 2_3:-48172548 None:intergenic
TTCGGTGTAAGAGAGAGCTG+TGG 0.694839 2_3:+48171667 Msa0295560:CDS
CTAGTAGGCAAGGCAAACAG+CGG 0.695275 2_3:+48171513 Msa0295560:five_prime_UTR
TGGATGAGAAGAACCCTCGT+CGG 0.698285 2_3:+48171745 Msa0295560:CDS
TCCTTCTCGTATGGACGACG+GGG 0.706036 2_3:-48171603 None:intergenic
CACCCTCAAAGATCCGACGA+GGG 0.706490 2_3:-48171758 None:intergenic
CTAACTCACCCACAAAACAA+CGG 0.711450 2_3:-48172875 None:intergenic
TCACCCTCAAAGATCCGACG+AGG 0.715310 2_3:-48171759 None:intergenic
CGGTGGTGACGAAAACAAGG+AGG 0.735551 2_3:+48172746 Msa0295560:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATTTAATTGTAAGTT+TGG + chr2_3:48172021-48172040 Msa0295560:intron 10.0%
!!! TTTTATATGTTAATTTTTTC+TGG + chr2_3:48172395-48172414 Msa0295560:intron 10.0%
!!! GTTTTAAAAACAAAATATAT+TGG - chr2_3:48172909-48172928 None:intergenic 10.0%
!! TATTTATTTAATTGTAAGTT+TGG + chr2_3:48172021-48172040 Msa0295560:intron 10.0%
!!! TTTTATATGTTAATTTTTTC+TGG + chr2_3:48172395-48172414 Msa0295560:intron 10.0%
!!! GTTTTAAAAACAAAATATAT+TGG - chr2_3:48172909-48172928 None:intergenic 10.0%
!!! ATATCATTTTTTGTTTTCAT+AGG + chr2_3:48172454-48172473 Msa0295560:intron 15.0%
!!! TATCATTTTTTGTTTTCATA+GGG + chr2_3:48172455-48172474 Msa0295560:intron 15.0%
!!! ATATCATTTTTTGTTTTCAT+AGG + chr2_3:48172454-48172473 Msa0295560:intron 15.0%
!!! TATCATTTTTTGTTTTCATA+GGG + chr2_3:48172455-48172474 Msa0295560:intron 15.0%
!! ATGTCAATAGAACTTACTTT+TGG + chr2_3:48172107-48172126 Msa0295560:intron 25.0%
!!! TGTCAATAGAACTTACTTTT+GGG + chr2_3:48172108-48172127 Msa0295560:intron 25.0%
!! GTAAAGGTTTAAGCAAATTT+TGG - chr2_3:48172302-48172321 None:intergenic 25.0%
! ATAAAAGGCAGAAATAAACT+AGG - chr2_3:48172346-48172365 None:intergenic 25.0%
!!! ATTTCTGCCTTTTATTTGAT+TGG + chr2_3:48172351-48172370 Msa0295560:intron 25.0%
!!! TTTCTGCCTTTTATTTGATT+GGG + chr2_3:48172352-48172371 Msa0295560:intron 25.0%
! AGAAAACCCAATCAAATAAA+AGG - chr2_3:48172361-48172380 None:intergenic 25.0%
!!! TAATTTTTTCTGGTAACCTA+TGG + chr2_3:48172405-48172424 Msa0295560:intron 25.0%
!!! AATTTTTTCTGGTAACCTAT+GGG + chr2_3:48172406-48172425 Msa0295560:intron 25.0%
! TATCAATCATATCCAAACAT+GGG - chr2_3:48172434-48172453 None:intergenic 25.0%
! TTATCAATCATATCCAAACA+TGG - chr2_3:48172435-48172454 None:intergenic 25.0%
!!! ATTTTTTGTTTTCATAGGGT+TGG + chr2_3:48172459-48172478 Msa0295560:intron 25.0%
!!! TTTTTTGTTTTCATAGGGTT+GGG + chr2_3:48172460-48172479 Msa0295560:intron 25.0%
! AATTAACTCACATATTCTCT+TGG + chr2_3:48172662-48172681 Msa0295560:three_prime_UTR 25.0%
! TTGTCTAGACATAAATTAGA+AGG - chr2_3:48172813-48172832 None:intergenic 25.0%
! GAACATCAAAGCAAAATATT+TGG - chr2_3:48172939-48172958 None:intergenic 25.0%
!! ATGTCAATAGAACTTACTTT+TGG + chr2_3:48172107-48172126 Msa0295560:intron 25.0%
!!! TGTCAATAGAACTTACTTTT+GGG + chr2_3:48172108-48172127 Msa0295560:intron 25.0%
!! GTAAAGGTTTAAGCAAATTT+TGG - chr2_3:48172302-48172321 None:intergenic 25.0%
! ATAAAAGGCAGAAATAAACT+AGG - chr2_3:48172346-48172365 None:intergenic 25.0%
!!! ATTTCTGCCTTTTATTTGAT+TGG + chr2_3:48172351-48172370 Msa0295560:intron 25.0%
!!! TTTCTGCCTTTTATTTGATT+GGG + chr2_3:48172352-48172371 Msa0295560:intron 25.0%
! AGAAAACCCAATCAAATAAA+AGG - chr2_3:48172361-48172380 None:intergenic 25.0%
!!! TAATTTTTTCTGGTAACCTA+TGG + chr2_3:48172405-48172424 Msa0295560:intron 25.0%
!!! AATTTTTTCTGGTAACCTAT+GGG + chr2_3:48172406-48172425 Msa0295560:intron 25.0%
! TATCAATCATATCCAAACAT+GGG - chr2_3:48172434-48172453 None:intergenic 25.0%
! TTATCAATCATATCCAAACA+TGG - chr2_3:48172435-48172454 None:intergenic 25.0%
!!! ATTTTTTGTTTTCATAGGGT+TGG + chr2_3:48172459-48172478 Msa0295560:intron 25.0%
!!! TTTTTTGTTTTCATAGGGTT+GGG + chr2_3:48172460-48172479 Msa0295560:intron 25.0%
! AATTAACTCACATATTCTCT+TGG + chr2_3:48172662-48172681 Msa0295560:three_prime_UTR 25.0%
! TTGTCTAGACATAAATTAGA+AGG - chr2_3:48172813-48172832 None:intergenic 25.0%
! GAACATCAAAGCAAAATATT+TGG - chr2_3:48172939-48172958 None:intergenic 25.0%
! ATTCCATTTTACCGAAACTA+TGG + chr2_3:48171564-48171583 Msa0295560:CDS 30.0%
! TTCCATTTTACCGAAACTAT+GGG + chr2_3:48171565-48171584 Msa0295560:CDS 30.0%
! ATCTGAGAGTTCCAATTTTT+GGG + chr2_3:48172065-48172084 Msa0295560:intron 30.0%
AACACAAAATTGTGCAAACA+GGG - chr2_3:48172151-48172170 None:intergenic 30.0%
AAACACAAAATTGTGCAAAC+AGG - chr2_3:48172152-48172171 None:intergenic 30.0%
ATCAATCATATCCAAACATG+GGG - chr2_3:48172433-48172452 None:intergenic 30.0%
TTGAAGTGAAAGGTTTCTTA+AGG + chr2_3:48172704-48172723 Msa0295560:intron 30.0%
! ATTCCATTTTACCGAAACTA+TGG + chr2_3:48171564-48171583 Msa0295560:CDS 30.0%
! TTCCATTTTACCGAAACTAT+GGG + chr2_3:48171565-48171584 Msa0295560:CDS 30.0%
! ATCTGAGAGTTCCAATTTTT+GGG + chr2_3:48172065-48172084 Msa0295560:intron 30.0%
AACACAAAATTGTGCAAACA+GGG - chr2_3:48172151-48172170 None:intergenic 30.0%
AAACACAAAATTGTGCAAAC+AGG - chr2_3:48172152-48172171 None:intergenic 30.0%
ATCAATCATATCCAAACATG+GGG - chr2_3:48172433-48172452 None:intergenic 30.0%
TTGAAGTGAAAGGTTTCTTA+AGG + chr2_3:48172704-48172723 Msa0295560:intron 30.0%
TTTCGGTAAAATGGAATGTG+GGG - chr2_3:48171561-48171580 None:intergenic 35.0%
GTTTCGGTAAAATGGAATGT+GGG - chr2_3:48171562-48171581 None:intergenic 35.0%
AGTTTCGGTAAAATGGAATG+TGG - chr2_3:48171563-48171582 None:intergenic 35.0%
TTCCCATAGTTTCGGTAAAA+TGG - chr2_3:48171570-48171589 None:intergenic 35.0%
! TGAAGGTTTTCCCATAGTTT+CGG - chr2_3:48171578-48171597 None:intergenic 35.0%
TTCTAGCATTGTTACGGATA+CGG - chr2_3:48171713-48171732 None:intergenic 35.0%
! AAAGGGTTCTAGCATTGTTA+CGG - chr2_3:48171719-48171738 None:intergenic 35.0%
CTTCTCATCCAATGTCAAAA+GGG - chr2_3:48171736-48171755 None:intergenic 35.0%
TCTTCTCATCCAATGTCAAA+AGG - chr2_3:48171737-48171756 None:intergenic 35.0%
TTGAGGAGAATGTTTAAGCA+TGG + chr2_3:48171789-48171808 Msa0295560:CDS 35.0%
! CATCTGAGAGTTCCAATTTT+TGG + chr2_3:48172064-48172083 Msa0295560:intron 35.0%
CACAAAGCAAACCCAAAAAT+TGG - chr2_3:48172079-48172098 None:intergenic 35.0%
AGTATAGACACGTAAAGACT+TGG - chr2_3:48172242-48172261 None:intergenic 35.0%
AGTCTTTACGTGTCTATACT+TGG + chr2_3:48172242-48172261 Msa0295560:intron 35.0%
!! GTTTAAGCAAATTTTGGCAG+TGG - chr2_3:48172296-48172315 None:intergenic 35.0%
GATCAGATAATCAACGGTAA+AGG - chr2_3:48172318-48172337 None:intergenic 35.0%
! GTCTAGGATCAGATAATCAA+CGG - chr2_3:48172324-48172343 None:intergenic 35.0%
!!! TCTCTTGGTTTTGTTCATAC+TGG + chr2_3:48172677-48172696 Msa0295560:three_prime_UTR 35.0%
CTAGACATAAATTAGAAGGC+AGG - chr2_3:48172809-48172828 None:intergenic 35.0%
!! ATTTTGCTACCGTTGTTTTG+TGG + chr2_3:48172866-48172885 Msa0295560:three_prime_UTR 35.0%
!! TTTTGCTACCGTTGTTTTGT+GGG + chr2_3:48172867-48172886 Msa0295560:three_prime_UTR 35.0%
TTTGCTTTGATGTTCTCTTC+AGG + chr2_3:48172944-48172963 Msa0295560:three_prime_UTR 35.0%
TTTCGGTAAAATGGAATGTG+GGG - chr2_3:48171561-48171580 None:intergenic 35.0%
GTTTCGGTAAAATGGAATGT+GGG - chr2_3:48171562-48171581 None:intergenic 35.0%
AGTTTCGGTAAAATGGAATG+TGG - chr2_3:48171563-48171582 None:intergenic 35.0%
TTCCCATAGTTTCGGTAAAA+TGG - chr2_3:48171570-48171589 None:intergenic 35.0%
! TGAAGGTTTTCCCATAGTTT+CGG - chr2_3:48171578-48171597 None:intergenic 35.0%
TTCTAGCATTGTTACGGATA+CGG - chr2_3:48171713-48171732 None:intergenic 35.0%
! AAAGGGTTCTAGCATTGTTA+CGG - chr2_3:48171719-48171738 None:intergenic 35.0%
CTTCTCATCCAATGTCAAAA+GGG - chr2_3:48171736-48171755 None:intergenic 35.0%
TCTTCTCATCCAATGTCAAA+AGG - chr2_3:48171737-48171756 None:intergenic 35.0%
TTGAGGAGAATGTTTAAGCA+TGG + chr2_3:48171789-48171808 Msa0295560:CDS 35.0%
! CATCTGAGAGTTCCAATTTT+TGG + chr2_3:48172064-48172083 Msa0295560:intron 35.0%
CACAAAGCAAACCCAAAAAT+TGG - chr2_3:48172079-48172098 None:intergenic 35.0%
AGTATAGACACGTAAAGACT+TGG - chr2_3:48172242-48172261 None:intergenic 35.0%
AGTCTTTACGTGTCTATACT+TGG + chr2_3:48172242-48172261 Msa0295560:intron 35.0%
!! GTTTAAGCAAATTTTGGCAG+TGG - chr2_3:48172296-48172315 None:intergenic 35.0%
GATCAGATAATCAACGGTAA+AGG - chr2_3:48172318-48172337 None:intergenic 35.0%
! GTCTAGGATCAGATAATCAA+CGG - chr2_3:48172324-48172343 None:intergenic 35.0%
!!! TCTCTTGGTTTTGTTCATAC+TGG + chr2_3:48172677-48172696 Msa0295560:three_prime_UTR 35.0%
CTAGACATAAATTAGAAGGC+AGG - chr2_3:48172809-48172828 None:intergenic 35.0%
!! ATTTTGCTACCGTTGTTTTG+TGG + chr2_3:48172866-48172885 Msa0295560:three_prime_UTR 35.0%
!! TTTTGCTACCGTTGTTTTGT+GGG + chr2_3:48172867-48172886 Msa0295560:three_prime_UTR 35.0%
TTTGCTTTGATGTTCTCTTC+AGG + chr2_3:48172944-48172963 Msa0295560:three_prime_UTR 35.0%
TTCACCACCAAAAACCTAGT+AGG + chr2_3:48171498-48171517 Msa0295560:five_prime_UTR 40.0%
! TGCTAGAACCCTTTTGACAT+TGG + chr2_3:48171725-48171744 Msa0295560:CDS 40.0%
GAACAAGCTCGATTATGTGT+TGG + chr2_3:48171830-48171849 Msa0295560:CDS 40.0%
TGAAGACAAGAGTCTGAAGA+CGG - chr2_3:48171884-48171903 None:intergenic 40.0%
CAGACTCTTGTCTTCAAATC+TGG + chr2_3:48171888-48171907 Msa0295560:CDS 40.0%
TCTTGTCTTCAAATCTGGCA+TGG + chr2_3:48171893-48171912 Msa0295560:CDS 40.0%
TTATCAGGCAGAGACACATT+AGG + chr2_3:48171943-48171962 Msa0295560:CDS 40.0%
CAGTGGCTACAAAACATGTA+TGG - chr2_3:48172279-48172298 None:intergenic 40.0%
GGCAGAAATAAACTAGGTCT+AGG - chr2_3:48172340-48172359 None:intergenic 40.0%
!! TTTGTTTTCATAGGGTTGGG+AGG + chr2_3:48172463-48172482 Msa0295560:intron 40.0%
AGTGAACATCCCATCCTTTA+TGG + chr2_3:48172491-48172510 Msa0295560:CDS 40.0%
TGAATCAACCCTCACCATAA+AGG - chr2_3:48172508-48172527 None:intergenic 40.0%
CTAACTCACCCACAAAACAA+CGG - chr2_3:48172878-48172897 None:intergenic 40.0%
TTCACCACCAAAAACCTAGT+AGG + chr2_3:48171498-48171517 Msa0295560:five_prime_UTR 40.0%
! TGCTAGAACCCTTTTGACAT+TGG + chr2_3:48171725-48171744 Msa0295560:CDS 40.0%
GAACAAGCTCGATTATGTGT+TGG + chr2_3:48171830-48171849 Msa0295560:CDS 40.0%
TGAAGACAAGAGTCTGAAGA+CGG - chr2_3:48171884-48171903 None:intergenic 40.0%
CAGACTCTTGTCTTCAAATC+TGG + chr2_3:48171888-48171907 Msa0295560:CDS 40.0%
TCTTGTCTTCAAATCTGGCA+TGG + chr2_3:48171893-48171912 Msa0295560:CDS 40.0%
TTATCAGGCAGAGACACATT+AGG + chr2_3:48171943-48171962 Msa0295560:CDS 40.0%
CAGTGGCTACAAAACATGTA+TGG - chr2_3:48172279-48172298 None:intergenic 40.0%
GGCAGAAATAAACTAGGTCT+AGG - chr2_3:48172340-48172359 None:intergenic 40.0%
!! TTTGTTTTCATAGGGTTGGG+AGG + chr2_3:48172463-48172482 Msa0295560:intron 40.0%
AGTGAACATCCCATCCTTTA+TGG + chr2_3:48172491-48172510 Msa0295560:CDS 40.0%
TGAATCAACCCTCACCATAA+AGG - chr2_3:48172508-48172527 None:intergenic 40.0%
CTAACTCACCCACAAAACAA+CGG - chr2_3:48172878-48172897 None:intergenic 40.0%
!! CTTGCCTACTAGGTTTTTGG+TGG - chr2_3:48171505-48171524 None:intergenic 45.0%
CACCAAAAACCTAGTAGGCA+AGG + chr2_3:48171503-48171522 Msa0295560:five_prime_UTR 45.0%
! TGCCTTGCCTACTAGGTTTT+TGG - chr2_3:48171508-48171527 None:intergenic 45.0%
! TTTGGATGCTGAGTTGAAGC+TGG + chr2_3:48171629-48171648 Msa0295560:CDS 45.0%
CAAGTCCATTCACCATGCTA+GGG + chr2_3:48171917-48171936 Msa0295560:CDS 45.0%
AAGAACCCTAGCATGGTGAA+TGG - chr2_3:48171925-48171944 None:intergenic 45.0%
ACCATGCTAGGGTTCTTATC+AGG + chr2_3:48171928-48171947 Msa0295560:CDS 45.0%
ACATCCCATCCTTTATGGTG+AGG + chr2_3:48172496-48172515 Msa0295560:CDS 45.0%
CATCCCATCCTTTATGGTGA+GGG + chr2_3:48172497-48172516 Msa0295560:CDS 45.0%
CAACCCTCACCATAAAGGAT+GGG - chr2_3:48172503-48172522 None:intergenic 45.0%
TCAACCCTCACCATAAAGGA+TGG - chr2_3:48172504-48172523 None:intergenic 45.0%
! TTTTCCCTCACTAGTCCCTT+TGG + chr2_3:48172543-48172562 Msa0295560:CDS 45.0%
CGTGAAGCGAAAGAACCTTA+AGG + chr2_3:48172584-48172603 Msa0295560:CDS 45.0%
TACTGGTCGCTTGAAGTGAA+AGG + chr2_3:48172694-48172713 Msa0295560:three_prime_UTR 45.0%
! TCTTAAGGCTGCTGCTTAGA+AGG + chr2_3:48172719-48172738 Msa0295560:intron 45.0%
!! CTTGCCTACTAGGTTTTTGG+TGG - chr2_3:48171505-48171524 None:intergenic 45.0%
CACCAAAAACCTAGTAGGCA+AGG + chr2_3:48171503-48171522 Msa0295560:five_prime_UTR 45.0%
! TGCCTTGCCTACTAGGTTTT+TGG - chr2_3:48171508-48171527 None:intergenic 45.0%
! TTTGGATGCTGAGTTGAAGC+TGG + chr2_3:48171629-48171648 Msa0295560:CDS 45.0%
CAAGTCCATTCACCATGCTA+GGG + chr2_3:48171917-48171936 Msa0295560:CDS 45.0%
AAGAACCCTAGCATGGTGAA+TGG - chr2_3:48171925-48171944 None:intergenic 45.0%
ACCATGCTAGGGTTCTTATC+AGG + chr2_3:48171928-48171947 Msa0295560:CDS 45.0%
ACATCCCATCCTTTATGGTG+AGG + chr2_3:48172496-48172515 Msa0295560:CDS 45.0%
CATCCCATCCTTTATGGTGA+GGG + chr2_3:48172497-48172516 Msa0295560:CDS 45.0%
CAACCCTCACCATAAAGGAT+GGG - chr2_3:48172503-48172522 None:intergenic 45.0%
TCAACCCTCACCATAAAGGA+TGG - chr2_3:48172504-48172523 None:intergenic 45.0%
! TTTTCCCTCACTAGTCCCTT+TGG + chr2_3:48172543-48172562 Msa0295560:CDS 45.0%
CGTGAAGCGAAAGAACCTTA+AGG + chr2_3:48172584-48172603 Msa0295560:CDS 45.0%
TACTGGTCGCTTGAAGTGAA+AGG + chr2_3:48172694-48172713 Msa0295560:three_prime_UTR 45.0%
! TCTTAAGGCTGCTGCTTAGA+AGG + chr2_3:48172719-48172738 Msa0295560:intron 45.0%
CTAGTAGGCAAGGCAAACAG+CGG + chr2_3:48171513-48171532 Msa0295560:five_prime_UTR 50.0%
!! TCCATACGAGAAGGAGCGTT+TGG + chr2_3:48171611-48171630 Msa0295560:CDS 50.0%
TCCAAACGCTCCTTCTCGTA+TGG - chr2_3:48171615-48171634 None:intergenic 50.0%
! GATGCTGAGTTGAAGCTGGT+CGG + chr2_3:48171633-48171652 Msa0295560:CDS 50.0%
TTGAAGCTGGTCGGAGAGTA+TGG + chr2_3:48171642-48171661 Msa0295560:CDS 50.0%
! TGGTCGGAGAGTATGGTCTT+CGG + chr2_3:48171649-48171668 Msa0295560:CDS 50.0%
! TTCGGTGTAAGAGAGAGCTG+TGG + chr2_3:48171667-48171686 Msa0295560:CDS 50.0%
GTGTAAGAGAGAGCTGTGGA+GGG + chr2_3:48171671-48171690 Msa0295560:CDS 50.0%
TGGATGAGAAGAACCCTCGT+CGG + chr2_3:48171745-48171764 Msa0295560:CDS 50.0%
AACCCTCGTCGGATCTTTGA+GGG + chr2_3:48171756-48171775 Msa0295560:CDS 50.0%
! TTGAGGGTGACGCACTTTTG+AGG + chr2_3:48171772-48171791 Msa0295560:CDS 50.0%
! CCTAGCATGGTGAATGGACT+TGG - chr2_3:48171919-48171938 None:intergenic 50.0%
CCAAGTCCATTCACCATGCT+AGG + chr2_3:48171916-48171935 Msa0295560:CDS 50.0%
GCCTGATAAGAACCCTAGCA+TGG - chr2_3:48171932-48171951 None:intergenic 50.0%
AACCTATGGGACCCCATGTT+TGG + chr2_3:48172419-48172438 Msa0295560:intron 50.0%
ATCCAAACATGGGGTCCCAT+AGG - chr2_3:48172424-48172443 None:intergenic 50.0%
! TTTTCATAGGGTTGGGAGGC+AGG + chr2_3:48172467-48172486 Msa0295560:intron 50.0%
! ACCACCAAAGGGACTAGTGA+GGG - chr2_3:48172550-48172569 None:intergenic 50.0%
ATCACCACCAGAAGCCTTCT+TGG - chr2_3:48172616-48172635 None:intergenic 50.0%
! AAGAAGGCTTCTGGTGGTGA+TGG + chr2_3:48172615-48172634 Msa0295560:CDS 50.0%
!! TTCTGGTGGTGATGGAGATG+AGG + chr2_3:48172623-48172642 Msa0295560:CDS 50.0%
! ATCCGGTGGTGACGAAAACA+AGG + chr2_3:48172743-48172762 Msa0295560:three_prime_UTR 50.0%
!!! CTCCTTGTTTTCGTCACCAC+CGG - chr2_3:48172748-48172767 None:intergenic 50.0%
CTAGTAGGCAAGGCAAACAG+CGG + chr2_3:48171513-48171532 Msa0295560:five_prime_UTR 50.0%
!! TCCATACGAGAAGGAGCGTT+TGG + chr2_3:48171611-48171630 Msa0295560:CDS 50.0%
TCCAAACGCTCCTTCTCGTA+TGG - chr2_3:48171615-48171634 None:intergenic 50.0%
! GATGCTGAGTTGAAGCTGGT+CGG + chr2_3:48171633-48171652 Msa0295560:CDS 50.0%
TTGAAGCTGGTCGGAGAGTA+TGG + chr2_3:48171642-48171661 Msa0295560:CDS 50.0%
! TGGTCGGAGAGTATGGTCTT+CGG + chr2_3:48171649-48171668 Msa0295560:CDS 50.0%
! TTCGGTGTAAGAGAGAGCTG+TGG + chr2_3:48171667-48171686 Msa0295560:CDS 50.0%
GTGTAAGAGAGAGCTGTGGA+GGG + chr2_3:48171671-48171690 Msa0295560:CDS 50.0%
TGGATGAGAAGAACCCTCGT+CGG + chr2_3:48171745-48171764 Msa0295560:CDS 50.0%
AACCCTCGTCGGATCTTTGA+GGG + chr2_3:48171756-48171775 Msa0295560:CDS 50.0%
! TTGAGGGTGACGCACTTTTG+AGG + chr2_3:48171772-48171791 Msa0295560:CDS 50.0%
! CCTAGCATGGTGAATGGACT+TGG - chr2_3:48171919-48171938 None:intergenic 50.0%
CCAAGTCCATTCACCATGCT+AGG + chr2_3:48171916-48171935 Msa0295560:CDS 50.0%
GCCTGATAAGAACCCTAGCA+TGG - chr2_3:48171932-48171951 None:intergenic 50.0%
AACCTATGGGACCCCATGTT+TGG + chr2_3:48172419-48172438 Msa0295560:intron 50.0%
ATCCAAACATGGGGTCCCAT+AGG - chr2_3:48172424-48172443 None:intergenic 50.0%
! TTTTCATAGGGTTGGGAGGC+AGG + chr2_3:48172467-48172486 Msa0295560:intron 50.0%
! ACCACCAAAGGGACTAGTGA+GGG - chr2_3:48172550-48172569 None:intergenic 50.0%
ATCACCACCAGAAGCCTTCT+TGG - chr2_3:48172616-48172635 None:intergenic 50.0%
! AAGAAGGCTTCTGGTGGTGA+TGG + chr2_3:48172615-48172634 Msa0295560:CDS 50.0%
!! TTCTGGTGGTGATGGAGATG+AGG + chr2_3:48172623-48172642 Msa0295560:CDS 50.0%
! ATCCGGTGGTGACGAAAACA+AGG + chr2_3:48172743-48172762 Msa0295560:three_prime_UTR 50.0%
!!! CTCCTTGTTTTCGTCACCAC+CGG - chr2_3:48172748-48172767 None:intergenic 50.0%
CGCTGTTTGCCTTGCCTACT+AGG - chr2_3:48171515-48171534 None:intergenic 55.0%
TCCTTCTCGTATGGACGACG+GGG - chr2_3:48171606-48171625 None:intergenic 55.0%
CTCCTTCTCGTATGGACGAC+GGG - chr2_3:48171607-48171626 None:intergenic 55.0%
GCTCCTTCTCGTATGGACGA+CGG - chr2_3:48171608-48171627 None:intergenic 55.0%
GGTGTAAGAGAGAGCTGTGG+AGG + chr2_3:48171670-48171689 Msa0295560:CDS 55.0%
GAACCCTCGTCGGATCTTTG+AGG + chr2_3:48171755-48171774 Msa0295560:CDS 55.0%
CACCCTCAAAGATCCGACGA+GGG - chr2_3:48171761-48171780 None:intergenic 55.0%
TCACCCTCAAAGATCCGACG+AGG - chr2_3:48171762-48171781 None:intergenic 55.0%
TCCCTCACTAGTCCCTTTGG+TGG + chr2_3:48172546-48172565 Msa0295560:CDS 55.0%
! CACCACCAAAGGGACTAGTG+AGG - chr2_3:48172551-48172570 None:intergenic 55.0%
CTCACTAGTCCCTTTGGTGG+TGG + chr2_3:48172549-48172568 Msa0295560:CDS 55.0%
CCAGGAAGACCACCACCAAA+GGG - chr2_3:48172561-48172580 None:intergenic 55.0%
ACCAGGAAGACCACCACCAA+AGG - chr2_3:48172562-48172581 None:intergenic 55.0%
TTCTTTCGCTTCACGCGACC+AGG - chr2_3:48172579-48172598 None:intergenic 55.0%
CCTTCTTGGCTGCAGCCTTA+AGG - chr2_3:48172602-48172621 None:intergenic 55.0%
CCTTAAGGCTGCAGCCAAGA+AGG + chr2_3:48172599-48172618 Msa0295560:CDS 55.0%
!! TGGTGGTGATGGAGATGAGG+AGG + chr2_3:48172626-48172645 Msa0295560:CDS 55.0%
!! GCTGCTGCTTAGAAGGCATC+CGG + chr2_3:48172726-48172745 Msa0295560:intron 55.0%
! CGGTGGTGACGAAAACAAGG+AGG + chr2_3:48172746-48172765 Msa0295560:three_prime_UTR 55.0%
CGCTGTTTGCCTTGCCTACT+AGG - chr2_3:48171515-48171534 None:intergenic 55.0%
TCCTTCTCGTATGGACGACG+GGG - chr2_3:48171606-48171625 None:intergenic 55.0%
CTCCTTCTCGTATGGACGAC+GGG - chr2_3:48171607-48171626 None:intergenic 55.0%
GCTCCTTCTCGTATGGACGA+CGG - chr2_3:48171608-48171627 None:intergenic 55.0%
GGTGTAAGAGAGAGCTGTGG+AGG + chr2_3:48171670-48171689 Msa0295560:CDS 55.0%
GAACCCTCGTCGGATCTTTG+AGG + chr2_3:48171755-48171774 Msa0295560:CDS 55.0%
CACCCTCAAAGATCCGACGA+GGG - chr2_3:48171761-48171780 None:intergenic 55.0%
TCACCCTCAAAGATCCGACG+AGG - chr2_3:48171762-48171781 None:intergenic 55.0%
TCCCTCACTAGTCCCTTTGG+TGG + chr2_3:48172546-48172565 Msa0295560:CDS 55.0%
! CACCACCAAAGGGACTAGTG+AGG - chr2_3:48172551-48172570 None:intergenic 55.0%
CTCACTAGTCCCTTTGGTGG+TGG + chr2_3:48172549-48172568 Msa0295560:CDS 55.0%
CCAGGAAGACCACCACCAAA+GGG - chr2_3:48172561-48172580 None:intergenic 55.0%
ACCAGGAAGACCACCACCAA+AGG - chr2_3:48172562-48172581 None:intergenic 55.0%
TTCTTTCGCTTCACGCGACC+AGG - chr2_3:48172579-48172598 None:intergenic 55.0%
CCTTCTTGGCTGCAGCCTTA+AGG - chr2_3:48172602-48172621 None:intergenic 55.0%
CCTTAAGGCTGCAGCCAAGA+AGG + chr2_3:48172599-48172618 Msa0295560:CDS 55.0%
!! TGGTGGTGATGGAGATGAGG+AGG + chr2_3:48172626-48172645 Msa0295560:CDS 55.0%
!! GCTGCTGCTTAGAAGGCATC+CGG + chr2_3:48172726-48172745 Msa0295560:intron 55.0%
! CGGTGGTGACGAAAACAAGG+AGG + chr2_3:48172746-48172765 Msa0295560:three_prime_UTR 55.0%
GTAGGCAAGGCAAACAGCGG+CGG + chr2_3:48171516-48171535 Msa0295560:five_prime_UTR 60.0%
TGGACGACGGGGCTTCTTGA+AGG - chr2_3:48171595-48171614 None:intergenic 60.0%
!! CCCTTTGGTGGTGGTCTTCC+TGG + chr2_3:48172558-48172577 Msa0295560:CDS 60.0%
GCTGCAGCCAAGAAGGCTTC+TGG + chr2_3:48172606-48172625 Msa0295560:CDS 60.0%
GCAGCCAAGAAGGCTTCTGG+TGG + chr2_3:48172609-48172628 Msa0295560:CDS 60.0%
!! GCTGCTTAGAAGGCATCCGG+TGG + chr2_3:48172729-48172748 Msa0295560:intron 60.0%
GTAGGCAAGGCAAACAGCGG+CGG + chr2_3:48171516-48171535 Msa0295560:five_prime_UTR 60.0%
TGGACGACGGGGCTTCTTGA+AGG - chr2_3:48171595-48171614 None:intergenic 60.0%
!! CCCTTTGGTGGTGGTCTTCC+TGG + chr2_3:48172558-48172577 Msa0295560:CDS 60.0%
GCTGCAGCCAAGAAGGCTTC+TGG + chr2_3:48172606-48172625 Msa0295560:CDS 60.0%
GCAGCCAAGAAGGCTTCTGG+TGG + chr2_3:48172609-48172628 Msa0295560:CDS 60.0%
!! GCTGCTTAGAAGGCATCCGG+TGG + chr2_3:48172729-48172748 Msa0295560:intron 60.0%
GCCCCGTCGTCCATACGAGA+AGG + chr2_3:48171602-48171621 Msa0295560:CDS 65.0%
GCCCCGTCGTCCATACGAGA+AGG + chr2_3:48171602-48171621 Msa0295560:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 48171493 48172991 48171493 ID=Msa0295560;Name=Msa0295560
chr2_3 mRNA 48171493 48172991 48171493 ID=Msa0295560-mRNA-1;Parent=Msa0295560;Name=Msa0295560-mRNA-1;_AED=0.13;_eAED=0.13;_QI=62|1|1|1|0.5|0.33|3|307|197
chr2_3 exon 48171493 48171964 48171493 ID=Msa0295560-mRNA-1:exon:15032;Parent=Msa0295560-mRNA-1
chr2_3 exon 48172476 48172715 48172476 ID=Msa0295560-mRNA-1:exon:15033;Parent=Msa0295560-mRNA-1
chr2_3 exon 48172741 48172991 48172741 ID=Msa0295560-mRNA-1:exon:15034;Parent=Msa0295560-mRNA-1
chr2_3 five_prime_UTR 48171493 48171554 48171493 ID=Msa0295560-mRNA-1:five_prime_utr;Parent=Msa0295560-mRNA-1
chr2_3 CDS 48171555 48171964 48171555 ID=Msa0295560-mRNA-1:cds;Parent=Msa0295560-mRNA-1
chr2_3 CDS 48172476 48172659 48172476 ID=Msa0295560-mRNA-1:cds;Parent=Msa0295560-mRNA-1
chr2_3 three_prime_UTR 48172660 48172715 48172660 ID=Msa0295560-mRNA-1:three_prime_utr;Parent=Msa0295560-mRNA-1
chr2_3 three_prime_UTR 48172741 48172991 48172741 ID=Msa0295560-mRNA-1:three_prime_utr;Parent=Msa0295560-mRNA-1
Gene Sequence

>Msa0295560

ATGCCCCACATTCCATTTTACCGAAACTATGGGAAAACCTTCAAGAAGCCCCGTCGTCCATACGAGAAGGAGCGTTTGGATGCTGAGTTGAAGCTGGTCGGAGAGTATGGTCTTCGGTGTAAGAGAGAGCTGTGGAGGGTTCAATACGCTCTGAGCCGTATCCGTAACAATGCTAGAACCCTTTTGACATTGGATGAGAAGAACCCTCGTCGGATCTTTGAGGGTGACGCACTTTTGAGGAGAATGTTTAAGCATGGTCTTCTTGACGAGACTCAGAACAAGCTCGATTATGTGTTGGCTCTCACTGTTGAGAATTTTCTCGAGCGCCGTCTTCAGACTCTTGTCTTCAAATCTGGCATGGCCAAGTCCATTCACCATGCTAGGGTTCTTATCAGGCAGAGACACATTAGGGTTGGGAGGCAGGTAGTGAACATCCCATCCTTTATGGTGAGGGTTGATTCACAGAAGCACATTGACTTTTCCCTCACTAGTCCCTTTGGTGGTGGTCTTCCTGGTCGCGTGAAGCGAAAGAACCTTAAGGCTGCAGCCAAGAAGGCTTCTGGTGGTGATGGAGATGAGGAGGATGAAGATTAA
Protein sequence

>Msa0295560

MPHIPFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYALSRIRNNARTLLTLDEKNPRRIFEGDALLRRMFKHGLLDETQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQVVNIPSFMVRVDSQKHIDFSLTSPFGGGLPGRVKRKNLKAAAKKASGGDGDEEDED*