AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0295690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295690 MtrunA17_Chr2g0317881 91.948 385 30 1 1 384 1 385 0.0 685
Msa0295690 MtrunA17_Chr2g0317871 81.748 389 66 2 1 384 1 389 0.0 617
Msa0295690 MtrunA17_Chr2g0281871 48.020 404 177 8 1 382 1 393 5.87e-117 345
Msa0295690 MtrunA17_Chr7g0249131 28.979 421 227 13 1 374 1 396 9.95e-34 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0295690 AT4G27460.1 52.538 394 169 9 1 382 1 388 3.05e-120 354
Msa0295690 AT5G53750.1 50.367 409 172 9 1 382 1 405 5.52e-117 346
Msa0295690 AT1G65320.1 27.635 427 228 13 1 374 1 399 5.04e-29 117

Find 101 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCTTGAATCTTCTTCT+AGG 0.311891 2_3:-48362700 Msa0295690:intron
TTATTTAGACCTATAGGATT+AGG 0.318448 2_3:-48361728 Msa0295690:exon
ACGAAACTTTCGTCAGTGTT+TGG 0.322278 2_3:-48362883 Msa0295690:CDS
TTACTATTCCCCTGCGTCTT+CGG 0.340419 2_3:-48362288 Msa0295690:CDS
TTCTAAAGCTCTTAAAGCTT+TGG 0.341556 2_3:-48362759 Msa0295690:CDS
TAATCAATGGCGAGCACATC+GGG 0.342837 2_3:+48362308 None:intergenic
AAGGTTGAAGGAGAAGAATT+TGG 0.348572 2_3:-48362054 Msa0295690:CDS
TAGAAGTTATTTAGACCTAT+AGG 0.349351 2_3:-48361734 Msa0295690:exon
AACTGTGACCATTCTGAATT+CGG 0.368428 2_3:-48362860 Msa0295690:CDS
TTTGTCCGCAGGGGACTTGA+TGG 0.380969 2_3:-48362129 Msa0295690:CDS
ACGTTGAAATAGACTTCAAC+GGG 0.384466 2_3:+48362948 None:intergenic
GACAGCAATCAAAGAGCTCT+TGG 0.386944 2_3:+48361869 None:intergenic
AGATAACTCATGTGTCAAAA+AGG 0.390942 2_3:+48362991 None:intergenic
TACATTTATCCTAATCCTAT+AGG 0.391553 2_3:+48361719 None:intergenic
AGGTTCTTGCTTGGTTCCAT+TGG 0.395496 2_3:-48362383 Msa0295690:CDS
AAAGCTTTAAGAGCTTTAGA+AGG 0.397669 2_3:+48362761 None:intergenic
TTCCGCGTTCATTCTTTGTA+TGG 0.417971 2_3:-48363020 Msa0295690:exon
GAGGAGAGTCCGACGAGGTC+AGG 0.420723 2_3:-48361960 Msa0295690:CDS
GAGGATGATTGCAGCTTAGT+TGG 0.425795 2_3:-48361801 Msa0295690:CDS
CGCAAGGTCATCGAGCTATT+CGG 0.427267 2_3:-48361931 Msa0295690:CDS
CTCATAGAGTGAACTACTTG+TGG 0.452341 2_3:-48361830 Msa0295690:CDS
TAGGGAAACATGAACCTTGT+TGG 0.455087 2_3:-48361671 Msa0295690:three_prime_UTR
GATGATGTCAAGAGCATCAA+TGG 0.458076 2_3:+48362334 None:intergenic
TCATAGAGTGAACTACTTGT+GGG 0.462004 2_3:-48361829 Msa0295690:CDS
AGCCATTGATCTTATACTCC+AGG 0.463049 2_3:-48362543 Msa0295690:CDS
TCGGTCGTTGTCAATGTGTT+GGG 0.467708 2_3:-48362841 Msa0295690:CDS
TTCTTTGACCGGTGATGTGT+TGG 0.469146 2_3:-48362000 Msa0295690:CDS
TTCAGCACCATTGTGGTTGT+TGG 0.483724 2_3:+48362436 None:intergenic
GATGTGATCAGGTTCTTGCT+TGG 0.488754 2_3:-48362392 Msa0295690:CDS
GAACTACTTGTGGGTTATTG+AGG 0.502090 2_3:-48361820 Msa0295690:CDS
ACAAGTCTCACAAGATCCTC+TGG 0.503032 2_3:+48362083 None:intergenic
GACAGTGATGGGACATTCAT+CGG 0.505323 2_3:-48362212 Msa0295690:CDS
AAGTTTCGTCGGAGCTTCTG+AGG 0.513062 2_3:+48362897 None:intergenic
TCCCTGGAGTATAAGATCAA+TGG 0.516231 2_3:+48362541 None:intergenic
ACTTGTAAAGGCAAGGTTGA+AGG 0.518293 2_3:-48362066 Msa0295690:CDS
TTCGGTCGTTGTCAATGTGT+TGG 0.520173 2_3:-48362842 Msa0295690:CDS
GCGATCACAACTTTGTCCGC+AGG 0.523015 2_3:-48362140 Msa0295690:CDS
ACAGCAATCAAAGAGCTCTT+GGG 0.523505 2_3:+48361870 None:intergenic
CTTCAACAGCCGAAGACGCA+GGG 0.535193 2_3:+48362279 None:intergenic
GACTTCAATTGCCTCGGCAA+CGG 0.536075 2_3:+48362919 None:intergenic
TCTGAGGACTTCAATTGCCT+CGG 0.537513 2_3:+48362913 None:intergenic
TTGCAATTGTCGACAGTGAT+GGG 0.541246 2_3:-48362223 Msa0295690:CDS
GTTGCAATTGTCGACAGTGA+TGG 0.542326 2_3:-48362224 Msa0295690:CDS
CGCAGGGGAATAGTAATCAA+TGG 0.542783 2_3:+48362295 None:intergenic
GCTTCAACAGCCGAAGACGC+AGG 0.544746 2_3:+48362278 None:intergenic
TAACACAAGAGGATGTGATC+AGG 0.550433 2_3:-48362403 Msa0295690:CDS
AGAAGGGCCACAACATTCAC+AGG 0.552296 2_3:+48361648 None:intergenic
AACGTTGGCTACCGTTGCCG+AGG 0.562278 2_3:-48362930 Msa0295690:CDS
TTGTGAGACTTGTAAAGGCA+AGG 0.564406 2_3:-48362073 Msa0295690:CDS
ACAACAAGACCATCAACCTT+AGG 0.565226 2_3:+48362722 None:intergenic
TCACAACATGCAAGCGTGAA+AGG 0.565398 2_3:+48362179 None:intergenic
CGAGGTCAGGAAAGCACGCA+AGG 0.570009 2_3:-48361947 Msa0295690:CDS
GCGTGCTTTCCTGACCTCGT+CGG 0.575337 2_3:+48361951 None:intergenic
ATGTGTTGGGAAAGTTTGCA+TGG 0.582379 2_3:-48362828 Msa0295690:CDS
GGATCTTGTGAGACTTGTAA+AGG 0.582402 2_3:-48362078 Msa0295690:CDS
TTGTGCAAGCGACTTTGAGA+TGG 0.582874 2_3:+48362256 None:intergenic
GTTGAAGTCTATTTCAACGT+TGG 0.583473 2_3:-48362945 Msa0295690:CDS
GTCGCTTGCACAACAAACTT+CGG 0.584024 2_3:-48362246 Msa0295690:CDS
GCCATTGATCTTATACTCCA+GGG 0.586242 2_3:-48362542 Msa0295690:CDS
GAGTGGAGTGAAGAGGCCAA+TGG 0.588041 2_3:+48362367 None:intergenic
GAGTTATCTGAACTCTGTCT+CGG 0.589665 2_3:-48362977 Msa0295690:CDS
AAGCGTTGAGAAAGTTGAAA+CGG 0.591594 2_3:+48363041 None:intergenic
GTCTCACAAGATCCTCTGGT+GGG 0.595536 2_3:+48362087 None:intergenic
ATGCAGAGAGTGGAGTGAAG+AGG 0.601482 2_3:+48362360 None:intergenic
ATTCTGTTGGTTAACACAAG+AGG 0.602333 2_3:-48362414 Msa0295690:CDS
CGATCACAACTTTGTCCGCA+GGG 0.602716 2_3:-48362139 Msa0295690:CDS
AGACGAGGCCAACACATCAC+CGG 0.605949 2_3:+48361992 None:intergenic
TCAATGGAGTATGCAGAGAG+TGG 0.607007 2_3:+48362350 None:intergenic
GACAACGACCGAATTCAGAA+TGG 0.612424 2_3:+48362852 None:intergenic
TGGGTCACCACATTCAATGT+AGG 0.612788 2_3:+48362106 None:intergenic
AACACTGACGAAAGTTTCGT+CGG 0.613598 2_3:+48362886 None:intergenic
TAATTGCAGTGTGGCTAATG+AGG 0.614165 2_3:-48361618 Msa0295690:three_prime_UTR
CGTCGGACTCTCCTCGTCAG+AGG 0.614308 2_3:+48361968 None:intergenic
AACAGAATTCAGCACCATTG+TGG 0.615037 2_3:+48362429 None:intergenic
AACGTTGAAATAGACTTCAA+CGG 0.620008 2_3:+48362947 None:intergenic
ACTGCAATTATCATAACAGA+AGG 0.624749 2_3:+48361631 None:intergenic
ACAACATTCACAGGCCAACA+AGG 0.625360 2_3:+48361657 None:intergenic
GTAATCAATGGCGAGCACAT+CGG 0.628862 2_3:+48362307 None:intergenic
CTCCTCGTCAGAGGAAGACG+AGG 0.630235 2_3:+48361977 None:intergenic
TGCGGACAAAGTTGTGATCG+CGG 0.639568 2_3:+48362142 None:intergenic
ACAATGGTGCTGAATTCTGT+TGG 0.643018 2_3:-48362427 Msa0295690:CDS
CCAGGGAGCACAAAATCTTG+TGG 0.643896 2_3:-48362525 Msa0295690:CDS
TTGTTGGCCTGTGAATGTTG+TGG 0.647356 2_3:-48361655 Msa0295690:three_prime_UTR
TCAACAACCAACAACCACAA+TGG 0.661007 2_3:-48362443 Msa0295690:CDS
AGTCTCACAAGATCCTCTGG+TGG 0.661172 2_3:+48362086 None:intergenic
TTCAACAGCCGAAGACGCAG+GGG 0.666030 2_3:+48362280 None:intergenic
GCTATTCGGCAAGATTTGTG+AGG 0.668957 2_3:-48361917 Msa0295690:CDS
GGCCTCGTCTTCCTCTGACG+AGG 0.669844 2_3:-48361979 Msa0295690:CDS
AAGATTTGTGAGGAATGTAG+AGG 0.675720 2_3:-48361907 Msa0295690:CDS
CTGCAATTATCATAACAGAA+GGG 0.681295 2_3:+48361632 None:intergenic
CTGTTATGATAATTGCAGTG+TGG 0.686000 2_3:-48361627 Msa0295690:three_prime_UTR
GCTTGCATGTTGTGATGAGA+CGG 0.686166 2_3:-48362171 Msa0295690:CDS
GTTGAAATAGACTTCAACGG+GGG 0.695337 2_3:+48362950 None:intergenic
TTGATGGCCTACATTGAATG+TGG 0.705015 2_3:-48362113 Msa0295690:CDS
TGAATGTGGTGACCCACCAG+AGG 0.705333 2_3:-48362099 Msa0295690:CDS
TGCCATACAAAGAATGAACG+CGG 0.722067 2_3:+48363018 None:intergenic
CTGACGAGGAGAGTCCGACG+AGG 0.740341 2_3:-48361965 Msa0295690:CDS
GATCACAACTTTGTCCGCAG+GGG 0.750662 2_3:-48362138 Msa0295690:CDS
AATCAATGGCGAGCACATCG+GGG 0.755270 2_3:+48362309 None:intergenic
GTAGGCCATCAAGTCCCCTG+CGG 0.766683 2_3:+48362124 None:intergenic
CGTTGAAATAGACTTCAACG+GGG 0.770457 2_3:+48362949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATCAAAATAACAATAATGA+GGG + chr2_3:48362024-48362043 None:intergenic 20.0%
!! ACATCAAAATAACAATAATG+AGG + chr2_3:48362025-48362044 None:intergenic 20.0%
!! CATCAAAATAACAATAATGA+GGG + chr2_3:48362024-48362043 None:intergenic 20.0%
!! ACATCAAAATAACAATAATG+AGG + chr2_3:48362025-48362044 None:intergenic 20.0%
!!! ATAATTTTGACTTCTTTGAC+CGG - chr2_3:48362591-48362610 Msa0295690:intron 25.0%
!! TACGTTTTCTAATATCTTGA+AGG - chr2_3:48362830-48362849 Msa0295690:CDS 25.0%
! TAGAAGTTATTTAGACCTAT+AGG - chr2_3:48362868-48362887 Msa0295690:CDS 25.0%
! TTATTTAGACCTATAGGATT+AGG - chr2_3:48362874-48362893 Msa0295690:CDS 25.0%
! TACATTTATCCTAATCCTAT+AGG + chr2_3:48362886-48362905 None:intergenic 25.0%
!!! ATGTAACAATGTTTTTGCTT+TGG - chr2_3:48362901-48362920 Msa0295690:CDS 25.0%
!!! TGTAACAATGTTTTTGCTTT+GGG - chr2_3:48362902-48362921 Msa0295690:CDS 25.0%
!!! ATAATTTTGACTTCTTTGAC+CGG - chr2_3:48362591-48362610 Msa0295690:intron 25.0%
!! TACGTTTTCTAATATCTTGA+AGG - chr2_3:48362830-48362849 Msa0295690:CDS 25.0%
! TAGAAGTTATTTAGACCTAT+AGG - chr2_3:48362868-48362887 Msa0295690:CDS 25.0%
! TTATTTAGACCTATAGGATT+AGG - chr2_3:48362874-48362893 Msa0295690:CDS 25.0%
! TACATTTATCCTAATCCTAT+AGG + chr2_3:48362886-48362905 None:intergenic 25.0%
!!! ATGTAACAATGTTTTTGCTT+TGG - chr2_3:48362901-48362920 Msa0295690:CDS 25.0%
!!! TGTAACAATGTTTTTGCTTT+GGG - chr2_3:48362902-48362921 Msa0295690:CDS 25.0%
AGATAACTCATGTGTCAAAA+AGG + chr2_3:48361614-48361633 None:intergenic 30.0%
AACGTTGAAATAGACTTCAA+CGG + chr2_3:48361658-48361677 None:intergenic 30.0%
!!! AAAGATTTTCTGGTTTGCAA+AGG + chr2_3:48361816-48361835 None:intergenic 30.0%
!! AAAGCTTTAAGAGCTTTAGA+AGG + chr2_3:48361844-48361863 None:intergenic 30.0%
!! TTCTAAAGCTCTTAAAGCTT+TGG - chr2_3:48361843-48361862 Msa0295690:CDS 30.0%
!! CCTCTTTTTGTTTGCAAAAT+TGG + chr2_3:48362105-48362124 None:intergenic 30.0%
! CCAATTTTGCAAACAAAAAG+AGG - chr2_3:48362102-48362121 Msa0295690:CDS 30.0%
! ATTTTGCAAACAAAAAGAGG+TGG - chr2_3:48362105-48362124 Msa0295690:CDS 30.0%
CTGCAATTATCATAACAGAA+GGG + chr2_3:48362973-48362992 None:intergenic 30.0%
ACTGCAATTATCATAACAGA+AGG + chr2_3:48362974-48362993 None:intergenic 30.0%
! CTTGACAAATTTTCTTGACA+AGG + chr2_3:48363064-48363083 None:intergenic 30.0%
AGATAACTCATGTGTCAAAA+AGG + chr2_3:48361614-48361633 None:intergenic 30.0%
AACGTTGAAATAGACTTCAA+CGG + chr2_3:48361658-48361677 None:intergenic 30.0%
!!! AAAGATTTTCTGGTTTGCAA+AGG + chr2_3:48361816-48361835 None:intergenic 30.0%
!! AAAGCTTTAAGAGCTTTAGA+AGG + chr2_3:48361844-48361863 None:intergenic 30.0%
!! TTCTAAAGCTCTTAAAGCTT+TGG - chr2_3:48361843-48361862 Msa0295690:CDS 30.0%
!! CCTCTTTTTGTTTGCAAAAT+TGG + chr2_3:48362105-48362124 None:intergenic 30.0%
! CCAATTTTGCAAACAAAAAG+AGG - chr2_3:48362102-48362121 Msa0295690:CDS 30.0%
! ATTTTGCAAACAAAAAGAGG+TGG - chr2_3:48362105-48362124 Msa0295690:CDS 30.0%
CTGCAATTATCATAACAGAA+GGG + chr2_3:48362973-48362992 None:intergenic 30.0%
ACTGCAATTATCATAACAGA+AGG + chr2_3:48362974-48362993 None:intergenic 30.0%
! CTTGACAAATTTTCTTGACA+AGG + chr2_3:48363064-48363083 None:intergenic 30.0%
!! AAGCGTTGAGAAAGTTGAAA+CGG + chr2_3:48361564-48361583 None:intergenic 35.0%
ACGTTGAAATAGACTTCAAC+GGG + chr2_3:48361657-48361676 None:intergenic 35.0%
GTTGAAGTCTATTTCAACGT+TGG - chr2_3:48361657-48361676 Msa0295690:three_prime_UTR 35.0%
AACTGTGACCATTCTGAATT+CGG - chr2_3:48361742-48361761 Msa0295690:exon 35.0%
! GAAGGACACAAAAGATTTTC+TGG + chr2_3:48361826-48361845 None:intergenic 35.0%
TTCAGCTTGAATCTTCTTCT+AGG - chr2_3:48361902-48361921 Msa0295690:CDS 35.0%
CAAAAAGAGGTGGTATTTCA+AGG - chr2_3:48362115-48362134 Msa0295690:CDS 35.0%
! ATTCTGTTGGTTAACACAAG+AGG - chr2_3:48362188-48362207 Msa0295690:CDS 35.0%
AAGGTTGAAGGAGAAGAATT+TGG - chr2_3:48362548-48362567 Msa0295690:CDS 35.0%
AAGATTTGTGAGGAATGTAG+AGG - chr2_3:48362695-48362714 Msa0295690:intron 35.0%
TCATAGAGTGAACTACTTGT+GGG - chr2_3:48362773-48362792 Msa0295690:CDS 35.0%
!! TTTTTGCTTTGGGTGTTCAT+AGG - chr2_3:48362912-48362931 Msa0295690:CDS 35.0%
!! TTTTGCTTTGGGTGTTCATA+GGG - chr2_3:48362913-48362932 Msa0295690:CDS 35.0%
CTGTTATGATAATTGCAGTG+TGG - chr2_3:48362975-48362994 Msa0295690:CDS 35.0%
!! AAGCGTTGAGAAAGTTGAAA+CGG + chr2_3:48361564-48361583 None:intergenic 35.0%
ACGTTGAAATAGACTTCAAC+GGG + chr2_3:48361657-48361676 None:intergenic 35.0%
GTTGAAGTCTATTTCAACGT+TGG - chr2_3:48361657-48361676 Msa0295690:three_prime_UTR 35.0%
AACTGTGACCATTCTGAATT+CGG - chr2_3:48361742-48361761 Msa0295690:exon 35.0%
! GAAGGACACAAAAGATTTTC+TGG + chr2_3:48361826-48361845 None:intergenic 35.0%
TTCAGCTTGAATCTTCTTCT+AGG - chr2_3:48361902-48361921 Msa0295690:CDS 35.0%
CAAAAAGAGGTGGTATTTCA+AGG - chr2_3:48362115-48362134 Msa0295690:CDS 35.0%
! ATTCTGTTGGTTAACACAAG+AGG - chr2_3:48362188-48362207 Msa0295690:CDS 35.0%
AAGGTTGAAGGAGAAGAATT+TGG - chr2_3:48362548-48362567 Msa0295690:CDS 35.0%
AAGATTTGTGAGGAATGTAG+AGG - chr2_3:48362695-48362714 Msa0295690:intron 35.0%
TCATAGAGTGAACTACTTGT+GGG - chr2_3:48362773-48362792 Msa0295690:CDS 35.0%
!! TTTTTGCTTTGGGTGTTCAT+AGG - chr2_3:48362912-48362931 Msa0295690:CDS 35.0%
!! TTTTGCTTTGGGTGTTCATA+GGG - chr2_3:48362913-48362932 Msa0295690:CDS 35.0%
CTGTTATGATAATTGCAGTG+TGG - chr2_3:48362975-48362994 Msa0295690:CDS 35.0%
TTCCGCGTTCATTCTTTGTA+TGG - chr2_3:48361582-48361601 Msa0295690:three_prime_UTR 40.0%
TGCCATACAAAGAATGAACG+CGG + chr2_3:48361587-48361606 None:intergenic 40.0%
GAGTTATCTGAACTCTGTCT+CGG - chr2_3:48361625-48361644 Msa0295690:three_prime_UTR 40.0%
GTTGAAATAGACTTCAACGG+GGG + chr2_3:48361655-48361674 None:intergenic 40.0%
CGTTGAAATAGACTTCAACG+GGG + chr2_3:48361656-48361675 None:intergenic 40.0%
AACACTGACGAAAGTTTCGT+CGG + chr2_3:48361719-48361738 None:intergenic 40.0%
ACGAAACTTTCGTCAGTGTT+TGG - chr2_3:48361719-48361738 Msa0295690:three_prime_UTR 40.0%
ATGTGTTGGGAAAGTTTGCA+TGG - chr2_3:48361774-48361793 Msa0295690:CDS 40.0%
!! GGTTTCTGTAGTTTTGCCTA+AGG - chr2_3:48361864-48361883 Msa0295690:CDS 40.0%
!! GTAGTTTTGCCTAAGGTTGA+TGG - chr2_3:48361871-48361890 Msa0295690:CDS 40.0%
ACAACAAGACCATCAACCTT+AGG + chr2_3:48361883-48361902 None:intergenic 40.0%
AGCCATTGATCTTATACTCC+AGG - chr2_3:48362059-48362078 Msa0295690:CDS 40.0%
GCCATTGATCTTATACTCCA+GGG - chr2_3:48362060-48362079 Msa0295690:CDS 40.0%
TCCCTGGAGTATAAGATCAA+TGG + chr2_3:48362064-48362083 None:intergenic 40.0%
TCAACAACCAACAACCACAA+TGG - chr2_3:48362159-48362178 Msa0295690:CDS 40.0%
AACAGAATTCAGCACCATTG+TGG + chr2_3:48362176-48362195 None:intergenic 40.0%
! ACAATGGTGCTGAATTCTGT+TGG - chr2_3:48362175-48362194 Msa0295690:CDS 40.0%
TAACACAAGAGGATGTGATC+AGG - chr2_3:48362199-48362218 Msa0295690:CDS 40.0%
!! GATGATGTCAAGAGCATCAA+TGG + chr2_3:48362271-48362290 None:intergenic 40.0%
TTGCAATTGTCGACAGTGAT+GGG - chr2_3:48362379-48362398 Msa0295690:CDS 40.0%
! TTGATGGCCTACATTGAATG+TGG - chr2_3:48362489-48362508 Msa0295690:CDS 40.0%
GGATCTTGTGAGACTTGTAA+AGG - chr2_3:48362524-48362543 Msa0295690:CDS 40.0%
TTGTGAGACTTGTAAAGGCA+AGG - chr2_3:48362529-48362548 Msa0295690:CDS 40.0%
ACTTGTAAAGGCAAGGTTGA+AGG - chr2_3:48362536-48362555 Msa0295690:CDS 40.0%
ACAGCAATCAAAGAGCTCTT+GGG + chr2_3:48362735-48362754 None:intergenic 40.0%
CTCATAGAGTGAACTACTTG+TGG - chr2_3:48362772-48362791 Msa0295690:CDS 40.0%
! GAACTACTTGTGGGTTATTG+AGG - chr2_3:48362782-48362801 Msa0295690:CDS 40.0%
TAGGGAAACATGAACCTTGT+TGG - chr2_3:48362931-48362950 Msa0295690:CDS 40.0%
TAATTGCAGTGTGGCTAATG+AGG - chr2_3:48362984-48363003 Msa0295690:CDS 40.0%
TTCCGCGTTCATTCTTTGTA+TGG - chr2_3:48361582-48361601 Msa0295690:three_prime_UTR 40.0%
TGCCATACAAAGAATGAACG+CGG + chr2_3:48361587-48361606 None:intergenic 40.0%
GAGTTATCTGAACTCTGTCT+CGG - chr2_3:48361625-48361644 Msa0295690:three_prime_UTR 40.0%
GTTGAAATAGACTTCAACGG+GGG + chr2_3:48361655-48361674 None:intergenic 40.0%
CGTTGAAATAGACTTCAACG+GGG + chr2_3:48361656-48361675 None:intergenic 40.0%
AACACTGACGAAAGTTTCGT+CGG + chr2_3:48361719-48361738 None:intergenic 40.0%
ACGAAACTTTCGTCAGTGTT+TGG - chr2_3:48361719-48361738 Msa0295690:three_prime_UTR 40.0%
ATGTGTTGGGAAAGTTTGCA+TGG - chr2_3:48361774-48361793 Msa0295690:CDS 40.0%
!! GGTTTCTGTAGTTTTGCCTA+AGG - chr2_3:48361864-48361883 Msa0295690:CDS 40.0%
!! GTAGTTTTGCCTAAGGTTGA+TGG - chr2_3:48361871-48361890 Msa0295690:CDS 40.0%
ACAACAAGACCATCAACCTT+AGG + chr2_3:48361883-48361902 None:intergenic 40.0%
AGCCATTGATCTTATACTCC+AGG - chr2_3:48362059-48362078 Msa0295690:CDS 40.0%
GCCATTGATCTTATACTCCA+GGG - chr2_3:48362060-48362079 Msa0295690:CDS 40.0%
TCCCTGGAGTATAAGATCAA+TGG + chr2_3:48362064-48362083 None:intergenic 40.0%
TCAACAACCAACAACCACAA+TGG - chr2_3:48362159-48362178 Msa0295690:CDS 40.0%
AACAGAATTCAGCACCATTG+TGG + chr2_3:48362176-48362195 None:intergenic 40.0%
! ACAATGGTGCTGAATTCTGT+TGG - chr2_3:48362175-48362194 Msa0295690:CDS 40.0%
TAACACAAGAGGATGTGATC+AGG - chr2_3:48362199-48362218 Msa0295690:CDS 40.0%
!! GATGATGTCAAGAGCATCAA+TGG + chr2_3:48362271-48362290 None:intergenic 40.0%
TTGCAATTGTCGACAGTGAT+GGG - chr2_3:48362379-48362398 Msa0295690:CDS 40.0%
! TTGATGGCCTACATTGAATG+TGG - chr2_3:48362489-48362508 Msa0295690:CDS 40.0%
GGATCTTGTGAGACTTGTAA+AGG - chr2_3:48362524-48362543 Msa0295690:CDS 40.0%
TTGTGAGACTTGTAAAGGCA+AGG - chr2_3:48362529-48362548 Msa0295690:CDS 40.0%
ACTTGTAAAGGCAAGGTTGA+AGG - chr2_3:48362536-48362555 Msa0295690:CDS 40.0%
ACAGCAATCAAAGAGCTCTT+GGG + chr2_3:48362735-48362754 None:intergenic 40.0%
CTCATAGAGTGAACTACTTG+TGG - chr2_3:48362772-48362791 Msa0295690:CDS 40.0%
! GAACTACTTGTGGGTTATTG+AGG - chr2_3:48362782-48362801 Msa0295690:CDS 40.0%
TAGGGAAACATGAACCTTGT+TGG - chr2_3:48362931-48362950 Msa0295690:CDS 40.0%
TAATTGCAGTGTGGCTAATG+AGG - chr2_3:48362984-48363003 Msa0295690:CDS 40.0%
TCTGAGGACTTCAATTGCCT+CGG + chr2_3:48361692-48361711 None:intergenic 45.0%
GACAACGACCGAATTCAGAA+TGG + chr2_3:48361753-48361772 None:intergenic 45.0%
TTCGGTCGTTGTCAATGTGT+TGG - chr2_3:48361760-48361779 Msa0295690:CDS 45.0%
TCGGTCGTTGTCAATGTGTT+GGG - chr2_3:48361761-48361780 Msa0295690:CDS 45.0%
TTCAGCACCATTGTGGTTGT+TGG + chr2_3:48362169-48362188 None:intergenic 45.0%
GATGTGATCAGGTTCTTGCT+TGG - chr2_3:48362210-48362229 Msa0295690:CDS 45.0%
AGGTTCTTGCTTGGTTCCAT+TGG - chr2_3:48362219-48362238 Msa0295690:CDS 45.0%
TCAATGGAGTATGCAGAGAG+TGG + chr2_3:48362255-48362274 None:intergenic 45.0%
TAATCAATGGCGAGCACATC+GGG + chr2_3:48362297-48362316 None:intergenic 45.0%
GTAATCAATGGCGAGCACAT+CGG + chr2_3:48362298-48362317 None:intergenic 45.0%
CGCAGGGGAATAGTAATCAA+TGG + chr2_3:48362310-48362329 None:intergenic 45.0%
TTACTATTCCCCTGCGTCTT+CGG - chr2_3:48362314-48362333 Msa0295690:CDS 45.0%
! TTGTGCAAGCGACTTTGAGA+TGG + chr2_3:48362349-48362368 None:intergenic 45.0%
GTCGCTTGCACAACAAACTT+CGG - chr2_3:48362356-48362375 Msa0295690:CDS 45.0%
GTTGCAATTGTCGACAGTGA+TGG - chr2_3:48362378-48362397 Msa0295690:CDS 45.0%
! GACAGTGATGGGACATTCAT+CGG - chr2_3:48362390-48362409 Msa0295690:CDS 45.0%
TCACAACATGCAAGCGTGAA+AGG + chr2_3:48362426-48362445 None:intergenic 45.0%
GCTTGCATGTTGTGATGAGA+CGG - chr2_3:48362431-48362450 Msa0295690:CDS 45.0%
TGGGTCACCACATTCAATGT+AGG + chr2_3:48362499-48362518 None:intergenic 45.0%
ACAAGTCTCACAAGATCCTC+TGG + chr2_3:48362522-48362541 None:intergenic 45.0%
TTCTTTGACCGGTGATGTGT+TGG - chr2_3:48362602-48362621 Msa0295690:intron 45.0%
GCTATTCGGCAAGATTTGTG+AGG - chr2_3:48362685-48362704 Msa0295690:intron 45.0%
GACAGCAATCAAAGAGCTCT+TGG + chr2_3:48362736-48362755 None:intergenic 45.0%
GAGGATGATTGCAGCTTAGT+TGG - chr2_3:48362801-48362820 Msa0295690:CDS 45.0%
ACAACATTCACAGGCCAACA+AGG + chr2_3:48362948-48362967 None:intergenic 45.0%
! TTGTTGGCCTGTGAATGTTG+TGG - chr2_3:48362947-48362966 Msa0295690:CDS 45.0%
TCTGAGGACTTCAATTGCCT+CGG + chr2_3:48361692-48361711 None:intergenic 45.0%
GACAACGACCGAATTCAGAA+TGG + chr2_3:48361753-48361772 None:intergenic 45.0%
TTCGGTCGTTGTCAATGTGT+TGG - chr2_3:48361760-48361779 Msa0295690:CDS 45.0%
TCGGTCGTTGTCAATGTGTT+GGG - chr2_3:48361761-48361780 Msa0295690:CDS 45.0%
TTCAGCACCATTGTGGTTGT+TGG + chr2_3:48362169-48362188 None:intergenic 45.0%
GATGTGATCAGGTTCTTGCT+TGG - chr2_3:48362210-48362229 Msa0295690:CDS 45.0%
AGGTTCTTGCTTGGTTCCAT+TGG - chr2_3:48362219-48362238 Msa0295690:CDS 45.0%
TCAATGGAGTATGCAGAGAG+TGG + chr2_3:48362255-48362274 None:intergenic 45.0%
TAATCAATGGCGAGCACATC+GGG + chr2_3:48362297-48362316 None:intergenic 45.0%
GTAATCAATGGCGAGCACAT+CGG + chr2_3:48362298-48362317 None:intergenic 45.0%
CGCAGGGGAATAGTAATCAA+TGG + chr2_3:48362310-48362329 None:intergenic 45.0%
TTACTATTCCCCTGCGTCTT+CGG - chr2_3:48362314-48362333 Msa0295690:CDS 45.0%
! TTGTGCAAGCGACTTTGAGA+TGG + chr2_3:48362349-48362368 None:intergenic 45.0%
GTCGCTTGCACAACAAACTT+CGG - chr2_3:48362356-48362375 Msa0295690:CDS 45.0%
GTTGCAATTGTCGACAGTGA+TGG - chr2_3:48362378-48362397 Msa0295690:CDS 45.0%
! GACAGTGATGGGACATTCAT+CGG - chr2_3:48362390-48362409 Msa0295690:CDS 45.0%
TCACAACATGCAAGCGTGAA+AGG + chr2_3:48362426-48362445 None:intergenic 45.0%
GCTTGCATGTTGTGATGAGA+CGG - chr2_3:48362431-48362450 Msa0295690:CDS 45.0%
TGGGTCACCACATTCAATGT+AGG + chr2_3:48362499-48362518 None:intergenic 45.0%
ACAAGTCTCACAAGATCCTC+TGG + chr2_3:48362522-48362541 None:intergenic 45.0%
TTCTTTGACCGGTGATGTGT+TGG - chr2_3:48362602-48362621 Msa0295690:intron 45.0%
GCTATTCGGCAAGATTTGTG+AGG - chr2_3:48362685-48362704 Msa0295690:intron 45.0%
GACAGCAATCAAAGAGCTCT+TGG + chr2_3:48362736-48362755 None:intergenic 45.0%
GAGGATGATTGCAGCTTAGT+TGG - chr2_3:48362801-48362820 Msa0295690:CDS 45.0%
ACAACATTCACAGGCCAACA+AGG + chr2_3:48362948-48362967 None:intergenic 45.0%
! TTGTTGGCCTGTGAATGTTG+TGG - chr2_3:48362947-48362966 Msa0295690:CDS 45.0%
GACTTCAATTGCCTCGGCAA+CGG + chr2_3:48361686-48361705 None:intergenic 50.0%
AAGTTTCGTCGGAGCTTCTG+AGG + chr2_3:48361708-48361727 None:intergenic 50.0%
! CCACAAGATTTTGTGCTCCC+TGG + chr2_3:48362080-48362099 None:intergenic 50.0%
! CCAGGGAGCACAAAATCTTG+TGG - chr2_3:48362077-48362096 Msa0295690:CDS 50.0%
ATGCAGAGAGTGGAGTGAAG+AGG + chr2_3:48362245-48362264 None:intergenic 50.0%
AATCAATGGCGAGCACATCG+GGG + chr2_3:48362296-48362315 None:intergenic 50.0%
TGCGGACAAAGTTGTGATCG+CGG + chr2_3:48362463-48362482 None:intergenic 50.0%
CGATCACAACTTTGTCCGCA+GGG - chr2_3:48362463-48362482 Msa0295690:CDS 50.0%
GATCACAACTTTGTCCGCAG+GGG - chr2_3:48362464-48362483 Msa0295690:CDS 50.0%
GTCTCACAAGATCCTCTGGT+GGG + chr2_3:48362518-48362537 None:intergenic 50.0%
AGTCTCACAAGATCCTCTGG+TGG + chr2_3:48362519-48362538 None:intergenic 50.0%
CGCAAGGTCATCGAGCTATT+CGG - chr2_3:48362671-48362690 Msa0295690:intron 50.0%
AGAAGGGCCACAACATTCAC+AGG + chr2_3:48362957-48362976 None:intergenic 50.0%
GACTTCAATTGCCTCGGCAA+CGG + chr2_3:48361686-48361705 None:intergenic 50.0%
AAGTTTCGTCGGAGCTTCTG+AGG + chr2_3:48361708-48361727 None:intergenic 50.0%
! CCACAAGATTTTGTGCTCCC+TGG + chr2_3:48362080-48362099 None:intergenic 50.0%
! CCAGGGAGCACAAAATCTTG+TGG - chr2_3:48362077-48362096 Msa0295690:CDS 50.0%
ATGCAGAGAGTGGAGTGAAG+AGG + chr2_3:48362245-48362264 None:intergenic 50.0%
AATCAATGGCGAGCACATCG+GGG + chr2_3:48362296-48362315 None:intergenic 50.0%
TGCGGACAAAGTTGTGATCG+CGG + chr2_3:48362463-48362482 None:intergenic 50.0%
CGATCACAACTTTGTCCGCA+GGG - chr2_3:48362463-48362482 Msa0295690:CDS 50.0%
GATCACAACTTTGTCCGCAG+GGG - chr2_3:48362464-48362483 Msa0295690:CDS 50.0%
GTCTCACAAGATCCTCTGGT+GGG + chr2_3:48362518-48362537 None:intergenic 50.0%
AGTCTCACAAGATCCTCTGG+TGG + chr2_3:48362519-48362538 None:intergenic 50.0%
CGCAAGGTCATCGAGCTATT+CGG - chr2_3:48362671-48362690 Msa0295690:intron 50.0%
AGAAGGGCCACAACATTCAC+AGG + chr2_3:48362957-48362976 None:intergenic 50.0%
GAGTGGAGTGAAGAGGCCAA+TGG + chr2_3:48362238-48362257 None:intergenic 55.0%
TTCAACAGCCGAAGACGCAG+GGG + chr2_3:48362325-48362344 None:intergenic 55.0%
CTTCAACAGCCGAAGACGCA+GGG + chr2_3:48362326-48362345 None:intergenic 55.0%
GCGATCACAACTTTGTCCGC+AGG - chr2_3:48362462-48362481 Msa0295690:CDS 55.0%
TTTGTCCGCAGGGGACTTGA+TGG - chr2_3:48362473-48362492 Msa0295690:CDS 55.0%
TGAATGTGGTGACCCACCAG+AGG - chr2_3:48362503-48362522 Msa0295690:CDS 55.0%
AGACGAGGCCAACACATCAC+CGG + chr2_3:48362613-48362632 None:intergenic 55.0%
GAGTGGAGTGAAGAGGCCAA+TGG + chr2_3:48362238-48362257 None:intergenic 55.0%
TTCAACAGCCGAAGACGCAG+GGG + chr2_3:48362325-48362344 None:intergenic 55.0%
CTTCAACAGCCGAAGACGCA+GGG + chr2_3:48362326-48362345 None:intergenic 55.0%
GCGATCACAACTTTGTCCGC+AGG - chr2_3:48362462-48362481 Msa0295690:CDS 55.0%
TTTGTCCGCAGGGGACTTGA+TGG - chr2_3:48362473-48362492 Msa0295690:CDS 55.0%
TGAATGTGGTGACCCACCAG+AGG - chr2_3:48362503-48362522 Msa0295690:CDS 55.0%
AGACGAGGCCAACACATCAC+CGG + chr2_3:48362613-48362632 None:intergenic 55.0%
! AACGTTGGCTACCGTTGCCG+AGG - chr2_3:48361672-48361691 Msa0295690:three_prime_UTR 60.0%
GCTTCAACAGCCGAAGACGC+AGG + chr2_3:48362327-48362346 None:intergenic 60.0%
GTAGGCCATCAAGTCCCCTG+CGG + chr2_3:48362481-48362500 None:intergenic 60.0%
CTCCTCGTCAGAGGAAGACG+AGG + chr2_3:48362628-48362647 None:intergenic 60.0%
GCGTGCTTTCCTGACCTCGT+CGG + chr2_3:48362654-48362673 None:intergenic 60.0%
! CGAGGTCAGGAAAGCACGCA+AGG - chr2_3:48362655-48362674 Msa0295690:intron 60.0%
! AACGTTGGCTACCGTTGCCG+AGG - chr2_3:48361672-48361691 Msa0295690:three_prime_UTR 60.0%
GCTTCAACAGCCGAAGACGC+AGG + chr2_3:48362327-48362346 None:intergenic 60.0%
GTAGGCCATCAAGTCCCCTG+CGG + chr2_3:48362481-48362500 None:intergenic 60.0%
CTCCTCGTCAGAGGAAGACG+AGG + chr2_3:48362628-48362647 None:intergenic 60.0%
GCGTGCTTTCCTGACCTCGT+CGG + chr2_3:48362654-48362673 None:intergenic 60.0%
! CGAGGTCAGGAAAGCACGCA+AGG - chr2_3:48362655-48362674 Msa0295690:intron 60.0%
GGCCTCGTCTTCCTCTGACG+AGG - chr2_3:48362623-48362642 Msa0295690:intron 65.0%
CGTCGGACTCTCCTCGTCAG+AGG + chr2_3:48362637-48362656 None:intergenic 65.0%
CTGACGAGGAGAGTCCGACG+AGG - chr2_3:48362637-48362656 Msa0295690:intron 65.0%
GAGGAGAGTCCGACGAGGTC+AGG - chr2_3:48362642-48362661 Msa0295690:intron 65.0%
GGCCTCGTCTTCCTCTGACG+AGG - chr2_3:48362623-48362642 Msa0295690:intron 65.0%
CGTCGGACTCTCCTCGTCAG+AGG + chr2_3:48362637-48362656 None:intergenic 65.0%
CTGACGAGGAGAGTCCGACG+AGG - chr2_3:48362637-48362656 Msa0295690:intron 65.0%
GAGGAGAGTCCGACGAGGTC+AGG - chr2_3:48362642-48362661 Msa0295690:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 48361529 48363095 48361529 ID=Msa0295690;Name=Msa0295690
chr2_3 mRNA 48361529 48363095 48361529 ID=Msa0295690-mRNA-1;Parent=Msa0295690;Name=Msa0295690-mRNA-1;_AED=0.04;_eAED=0.04;_QI=72|1|1|1|1|1|2|214|384
chr2_3 exon 48361529 48362574 48361529 ID=Msa0295690-mRNA-1:exon:15186;Parent=Msa0295690-mRNA-1
chr2_3 exon 48362701 48363095 48362701 ID=Msa0295690-mRNA-1:exon:15185;Parent=Msa0295690-mRNA-1
chr2_3 five_prime_UTR 48363024 48363095 48363024 ID=Msa0295690-mRNA-1:five_prime_utr;Parent=Msa0295690-mRNA-1
chr2_3 CDS 48362701 48363023 48362701 ID=Msa0295690-mRNA-1:cds;Parent=Msa0295690-mRNA-1
chr2_3 CDS 48361743 48362574 48361743 ID=Msa0295690-mRNA-1:cds;Parent=Msa0295690-mRNA-1
chr2_3 three_prime_UTR 48361529 48361742 48361529 ID=Msa0295690-mRNA-1:three_prime_utr;Parent=Msa0295690-mRNA-1
Gene Sequence

>Msa0295690

ATGGCAGTATCCTTTTTGACACATGAGTTATCTGAACTCTGTCTCGGTAAGCCCCCGTTGAAGTCTATTTCAACGTTGGCTACCGTTGCCGAGGCAATTGAAGTCCTCAGAAGCTCCGACGAAACTTTCGTCAGTGTTTGGAACTGTGACCATTCTGAATTCGGTCGTTGTCAATGTGTTGGGAAAGTTTGCATGGTTGATGTTATTTGTTACCTTTGCAAACCAGAAAATCTTTTGTGTCCTTCTAAAGCTCTTAAAGCTTTGGTTTCTGTAGTTTTGCCTAAGGTTGATGGTCTTGTTGTTCAGCTTGAATCTTCTTCTAGCTTATTAGAAGCCATTGATCTTATACTCCAGGGAGCACAAAATCTTGTGGTGCCAATTTTGCAAACAAAAAGAGGTGGTATTTCAAGGAGAAAACATCAACAAAAGTCATCAACAACCAACAACCACAATGGTGCTGAATTCTGTTGGTTAACACAAGAGGATGTGATCAGGTTCTTGCTTGGTTCCATTGGCCTCTTCACTCCACTCTCTGCATACTCCATTGATGCTCTTGACATCATCATCCCCGATGTGCTCGCCATTGATTACTATTCCCCTGCGTCTTCGGCTGTTGAAGCCATCTCAAAGTCGCTTGCACAACAAACTTCGGTTGCAATTGTCGACAGTGATGGGACATTCATCGGTGAGATATCGCCTTTCACGCTTGCATGTTGTGATGAGACGGTAGCTGCCGCGATCACAACTTTGTCCGCAGGGGACTTGATGGCCTACATTGAATGTGGTGACCCACCAGAGGATCTTGTGAGACTTGTAAAGGCAAGGTTGAAGGAGAAGAATTTGGAGAAATTGCTACAAGAGTTTATAATTTTGACTTCTTTGACCGGTGATGTGTTGGCCTCGTCTTCCTCTGACGAGGAGAGTCCGACGAGGTCAGGAAAGCACGCAAGGTCATCGAGCTATTCGGCAAGATTTGTGAGGAATGTAGAGGCTATAGTTTGTCATCCCAAGAGCTCTTTGATTGCTGTCATGATTCAAGCAGTTTCTCATAGAGTGAACTACTTGTGGGTTATTGAGGATGATTGCAGCTTAGTTGGTATTGTTACGTTTTCTAATATCTTGAAGGTGTTCAGAGAATGTTTAGAAGTTATTTAG
Protein sequence

>Msa0295690

MAVSFLTHELSELCLGKPPLKSISTLATVAEAIEVLRSSDETFVSVWNCDHSEFGRCQCVGKVCMVDVICYLCKPENLLCPSKALKALVSVVLPKVDGLVVQLESSSSLLEAIDLILQGAQNLVVPILQTKRGGISRRKHQQKSSTTNNHNGAEFCWLTQEDVIRFLLGSIGLFTPLSAYSIDALDIIIPDVLAIDYYSPASSAVEAISKSLAQQTSVAIVDSDGTFIGEISPFTLACCDETVAAAITTLSAGDLMAYIECGDPPEDLVRLVKARLKEKNLEKLLQEFIILTSLTGDVLASSSSDEESPTRSGKHARSSSYSARFVRNVEAIVCHPKSSLIAVMIQAVSHRVNYLWVIEDDCSLVGIVTFSNILKVFRECLEVI*