AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0298850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0298850 MtrunA17_Chr7g0259221 74.691 486 25 7 1 399 1 475 0.0 667
Msa0298850 MtrunA17_Chr7g0259471 71.730 474 56 7 3 399 1 473 0.0 630
Msa0298850 MtrunA17_Chr4g0066581 46.061 495 166 13 1 401 1 488 1.45e-127 376
Msa0298850 MtrunA17_Chr2g0286721 45.082 488 178 11 1 401 1 485 4.34e-123 365
Msa0298850 MtrunA17_Chr7g0273281 39.583 480 194 9 15 401 21 497 2.50e-94 291
Msa0298850 MtrunA17_Chr8g0373181 38.095 441 182 9 42 396 47 482 4.82e-81 257
Msa0298850 MtrunA17_Chr1g0155221 38.568 433 182 10 47 396 54 485 2.51e-80 255
Msa0298850 MtrunA17_Chr8g0373191 34.583 480 199 14 11 400 4 458 1.02e-64 213
Msa0298850 MtrunA17_Chr7g0273261 38.739 222 91 5 219 399 20 237 4.06e-43 151
Msa0298850 MtrunA17_Chr5g0404191 35.521 259 126 6 5 224 4 260 4.73e-40 144
Msa0298850 MtrunA17_Chr7g0273271 43.716 183 71 4 41 193 26 206 2.30e-31 119
Msa0298850 MtrunA17_Chr7g0221631 58.025 81 32 1 286 366 39 117 1.13e-23 95.1
Msa0298850 MtrunA17_Chr7g0250881 23.908 435 245 11 48 399 71 502 7.51e-23 100
Msa0298850 MtrunA17_Chr6g0457781 24.714 437 243 11 48 401 111 544 9.46e-23 100
Msa0298850 MtrunA17_Chr5g0404181 47.727 88 44 1 309 396 43 128 1.67e-22 92.4
Msa0298850 MtrunA17_Chr3g0115721 25.183 409 218 9 73 395 77 483 7.55e-22 97.4
Msa0298850 MtrunA17_Chr4g0032021 25.104 482 249 17 26 400 79 555 9.92e-20 91.3
Msa0298850 MtrunA17_Chr1g0213631 27.163 416 212 16 68 396 18 429 1.64e-19 90.1
Msa0298850 MtrunA17_Chr3g0115751 26.034 411 212 11 73 395 82 488 2.17e-19 90.1
Msa0298850 MtrunA17_Chr3g0115741 25.432 405 212 9 78 395 85 486 3.58e-19 89.4
Msa0298850 MtrunA17_Chr7g0259451 73.077 52 12 1 313 364 1 50 8.48e-19 79.3
Msa0298850 MtrunA17_Chr3g0115731 24.679 389 224 10 73 395 77 462 1.44e-18 87.4
Msa0298850 MtrunA17_Chr3g0115781 23.267 404 224 7 78 396 84 486 1.54e-18 87.4
Msa0298850 MtrunA17_Chr4g0039651 24.453 503 250 18 6 397 15 498 1.73e-18 87.4
Msa0298850 MtrunA17_Chr6g0486021 24.161 447 232 10 47 400 55 487 2.12e-18 87.0
Msa0298850 MtrunA17_Chr5g0432581 23.140 484 252 18 23 396 6 479 4.47e-17 82.8
Msa0298850 MtrunA17_Chr1g0213611 23.769 467 234 14 37 396 74 525 6.04e-17 82.8
Msa0298850 MtrunA17_Chr4g0004821 25.309 486 258 16 9 401 1 474 5.28e-15 76.6
Msa0298850 MtrunA17_Chr7g0214451 24.745 392 195 15 96 396 94 476 3.32e-13 70.9
Msa0298850 MtrunA17_Chr8g0373531 21.351 459 247 13 46 396 27 479 1.52e-12 68.9
Msa0298850 MtrunA17_Chr5g0437021 26.023 342 196 11 73 367 79 410 3.45e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0298850 AT2G48020.2 55.914 465 118 9 14 399 5 461 5.89e-165 471
Msa0298850 AT2G48020.1 55.914 465 118 9 14 399 5 461 5.89e-165 471
Msa0298850 AT2G48020.4 55.108 372 100 8 14 326 5 368 2.43e-123 362
Msa0298850 AT5G18840.1 45.322 481 176 11 3 399 1 478 7.79e-122 362
Msa0298850 AT3G05150.2 42.706 473 186 9 6 400 5 470 6.12e-113 339
Msa0298850 AT3G05150.1 42.495 473 187 9 6 400 5 470 1.45e-112 338
Msa0298850 AT3G05150.3 42.495 473 187 9 6 400 5 470 1.45e-112 338
Msa0298850 AT2G48020.3 57.729 317 88 7 17 295 8 316 2.21e-110 328
Msa0298850 AT1G54730.5 43.521 409 174 7 47 399 37 444 2.06e-103 313
Msa0298850 AT1G54730.2 41.395 430 174 8 47 399 37 465 2.17e-98 301
Msa0298850 AT1G54730.4 43.850 374 153 7 47 364 37 409 3.26e-91 281
Msa0298850 AT1G54730.3 41.519 395 153 8 47 364 37 430 5.28e-86 268
Msa0298850 AT1G08920.1 37.671 438 191 9 41 399 31 465 1.13e-85 268
Msa0298850 AT1G08920.2 37.528 445 189 9 41 399 31 472 2.53e-85 268
Msa0298850 AT1G19450.1 36.735 490 210 12 3 397 1 485 2.67e-84 266
Msa0298850 AT3G05155.1 36.830 448 207 8 24 396 9 455 3.55e-84 264
Msa0298850 AT1G75220.1 36.531 490 210 13 3 397 1 484 1.13e-83 264
Msa0298850 AT3G20460.1 37.684 475 207 12 2 399 24 486 9.89e-82 259
Msa0298850 AT1G08930.1 37.182 433 194 9 42 397 58 489 4.39e-80 255
Msa0298850 AT1G08930.2 37.182 433 194 9 42 397 58 489 4.39e-80 255
Msa0298850 AT3G05165.3 33.836 464 224 8 13 399 6 463 2.48e-79 252
Msa0298850 AT3G05165.2 33.836 464 224 8 13 399 6 463 2.48e-79 252
Msa0298850 AT3G05165.1 33.836 464 224 8 13 399 6 463 2.48e-79 252
Msa0298850 AT3G05160.1 34.783 460 217 8 17 399 1 454 3.22e-79 251
Msa0298850 AT5G27350.1 36.301 438 197 9 42 400 31 465 5.93e-77 246
Msa0298850 AT1G54730.8 44.795 317 138 6 47 327 37 352 1.89e-76 242
Msa0298850 AT4G04750.1 34.038 473 217 10 7 398 12 470 2.02e-75 242
Msa0298850 AT4G04750.2 34.038 473 217 10 7 398 12 470 2.02e-75 242
Msa0298850 AT5G27360.1 34.690 467 220 10 13 400 9 469 4.28e-75 241
Msa0298850 AT3G05400.1 35.205 463 212 11 23 401 3 461 6.24e-75 241
Msa0298850 AT4G04750.3 34.038 473 217 10 7 398 56 514 8.01e-75 242
Msa0298850 AT5G27360.3 34.690 467 220 10 13 400 57 517 3.12e-74 241
Msa0298850 AT4G04760.1 36.199 442 195 10 38 397 29 465 2.43e-73 237
Msa0298850 AT1G08920.3 36.973 403 172 9 41 364 31 430 3.18e-73 236
Msa0298850 AT1G54730.9 42.012 338 138 7 47 327 37 373 3.40e-71 229
Msa0298850 AT1G54730.7 42.012 338 138 7 47 327 37 373 3.40e-71 229
Msa0298850 AT1G54730.10 47.670 279 109 6 47 289 37 314 1.71e-70 225
Msa0298850 AT1G54730.6 47.670 279 109 6 47 289 37 314 1.71e-70 225
Msa0298850 AT1G54730.11 47.122 278 110 6 47 288 37 313 1.63e-69 223
Msa0298850 AT3G05160.3 35.443 395 181 6 78 399 12 405 3.45e-69 224
Msa0298850 AT3G05160.2 35.443 395 181 6 78 399 12 405 3.45e-69 224
Msa0298850 AT4G04760.2 35.731 431 191 10 48 397 37 462 4.65e-67 220
Msa0298850 AT3G05165.6 32.258 434 211 8 13 369 6 433 1.36e-64 215
Msa0298850 AT4G04760.3 36.029 408 176 9 72 397 2 406 2.24e-64 212
Msa0298850 AT3G05400.2 33.477 463 200 12 23 401 3 441 2.55e-64 212
Msa0298850 AT1G08890.1 31.897 464 226 11 17 399 6 460 2.20e-62 208
Msa0298850 AT1G08900.1 33.333 435 208 9 45 400 28 459 3.31e-62 207
Msa0298850 AT1G08900.2 33.333 435 208 9 45 400 28 459 3.31e-62 207
Msa0298850 AT1G08900.5 33.493 418 196 9 62 400 3 417 4.75e-59 198
Msa0298850 AT1G08900.3 32.483 431 209 9 45 400 28 451 9.18e-58 196
Msa0298850 AT3G05155.2 38.591 298 149 5 24 288 9 305 1.30e-57 191
Msa0298850 AT3G05165.4 31.719 413 199 8 13 348 6 412 7.17e-57 193
Msa0298850 AT3G05165.5 31.719 413 199 8 13 348 6 412 7.17e-57 193
Msa0298850 AT5G27360.2 33.696 368 166 8 108 400 1 365 9.80e-55 185
Msa0298850 AT5G27360.5 33.696 368 166 8 108 400 1 365 9.80e-55 185
Msa0298850 AT1G08900.4 33.083 399 185 9 45 364 28 423 2.65e-49 172
Msa0298850 AT5G27360.4 36.218 312 159 7 13 288 9 316 5.99e-49 172
Msa0298850 AT5G16150.2 24.208 442 239 10 48 401 111 544 1.62e-22 100
Msa0298850 AT5G16150.1 24.208 442 239 10 48 401 111 544 1.62e-22 100
Msa0298850 AT5G16150.3 24.208 442 239 10 48 401 111 544 1.62e-22 100
Msa0298850 AT5G59250.1 26.471 408 204 14 86 400 153 557 9.81e-21 94.7
Msa0298850 AT1G79820.2 25.885 452 248 12 34 401 44 492 3.25e-20 92.8
Msa0298850 AT1G79820.1 25.885 452 248 12 34 401 44 492 3.25e-20 92.8
Msa0298850 AT3G03090.1 26.069 491 253 19 8 397 19 500 6.84e-19 89.0
Msa0298850 AT5G17010.1 26.349 482 243 18 18 397 29 500 1.25e-18 88.2
Msa0298850 AT5G17010.3 26.349 482 243 18 18 397 29 500 1.25e-18 88.2
Msa0298850 AT5G17010.4 26.349 482 243 18 18 397 29 500 1.25e-18 88.2
Msa0298850 AT1G67300.1 25.166 453 224 15 47 401 56 491 1.36e-18 87.8
Msa0298850 AT1G67300.3 25.166 453 224 15 47 401 56 491 1.36e-18 87.8
Msa0298850 AT1G67300.2 25.110 454 224 15 47 401 56 492 1.49e-18 87.8
Msa0298850 AT1G79820.4 26.757 370 209 9 34 342 44 412 2.30e-17 84.0
Msa0298850 AT1G54730.1 60.656 61 24 0 89 149 1 61 2.52e-17 76.3
Msa0298850 AT1G67300.4 27.645 293 158 7 47 291 56 342 7.28e-17 82.4
Msa0298850 AT1G67300.5 27.645 293 158 7 47 291 56 342 7.28e-17 82.4
Msa0298850 AT1G67300.6 27.645 293 158 7 47 291 56 342 8.32e-17 82.4
Msa0298850 AT1G67300.7 27.645 293 158 7 47 291 56 342 8.32e-17 82.4
Msa0298850 AT2G43330.1 25.442 283 146 10 174 396 199 476 1.31e-15 79.0
Msa0298850 AT5G17010.2 25.670 448 229 16 18 397 26 437 4.09e-15 77.0
Msa0298850 AT1G05030.1 25.339 442 235 15 48 401 85 519 2.08e-14 75.1
Msa0298850 AT1G05030.2 24.944 449 237 16 40 401 32 467 3.81e-14 74.3

Find 130 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCACTGCTGTGTTGCTTC+TGG 0.130660 2_3:+53052335 Msa0298850:CDS
CTTGGTTTCAATTAGGTATC+TGG 0.196736 2_3:+53053515 Msa0298850:intron
CTTATGGTTTGCCAGCAATT+AGG 0.210158 2_3:+53053000 Msa0298850:CDS
GATATTTCCAGTCAATATTA+AGG 0.214954 2_3:+53054118 Msa0298850:intron
TTATGGTTTGCCAGCAATTA+GGG 0.246534 2_3:+53053001 Msa0298850:CDS
TTAATAGGGTCCAGTGTCTT+TGG 0.269057 2_3:+53051783 Msa0298850:intron
GTGTAGTGTTTGGTGGAAAT+TGG 0.274589 2_3:+53054509 Msa0298850:three_prime_UTR
TATGATTGTGACTGTGTTAT+AGG 0.275527 2_3:-53050677 None:intergenic
AGGGCACCTCAGAGTTCTTT+TGG 0.283972 2_3:-53051975 None:intergenic
CCCACTGCTGTGTTGCTTCT+GGG 0.289747 2_3:+53052336 Msa0298850:CDS
GGTCCAGTGTCTTTGGATAT+TGG 0.293427 2_3:+53051790 Msa0298850:CDS
CCATACCAATATGCCGTTTA+CGG 0.297005 2_3:-53053710 None:intergenic
GCAACACAGCAGTGGGAATA+AGG 0.301338 2_3:-53052329 None:intergenic
GTCCAGTGTCTTTGGATATT+GGG 0.312516 2_3:+53051791 Msa0298850:CDS
GGAAATGCATTATAATATTA+AGG 0.313911 2_3:-53054693 None:intergenic
TAAAAGCCTTGAACAATTAC+AGG 0.318980 2_3:+53054455 Msa0298850:CDS
CTCCAACTCAAGAAGCTATT+AGG 0.337233 2_3:+53051094 Msa0298850:CDS
ACCACATTCATTGCAGTTTG+TGG 0.345828 2_3:+53050878 Msa0298850:CDS
GCAGTACTCCACTCCTTGTT+TGG 0.360918 2_3:+53051434 Msa0298850:intron
ACATGTGGTTTCATAAAGAA+TGG 0.363136 2_3:+53054743 Msa0298850:three_prime_UTR
GCAAACTTCCTACGAGATAA+TGG 0.365088 2_3:-53054481 None:intergenic
GTAGATTGGAGTAGGGCTTA+TGG 0.368425 2_3:+53052984 Msa0298850:intron
GGGAGACTAGCAACAGGATA+TGG 0.368465 2_3:+53051811 Msa0298850:CDS
TTCAATTAGGTATCTGGATC+AGG 0.372298 2_3:+53053521 Msa0298850:intron
GGAATAAGAGAACCATTGAT+AGG 0.373744 2_3:+53050791 Msa0298850:CDS
CCCCTTCCTTCCAACGAAAT+CGG 0.378643 2_3:-53051510 None:intergenic
TGAAGAGGCAGAAGAAATTC+AGG 0.379612 2_3:+53052566 Msa0298850:CDS
GTCCCAGCACTTGCCGTAAA+CGG 0.389811 2_3:+53053697 Msa0298850:CDS
GTGGAAGGTGGCAAACAAAA+AGG 0.390224 2_3:+53050770 Msa0298850:CDS
GTTTGTGGTTCTTATGAGTT+TGG 0.394244 2_3:+53050893 Msa0298850:CDS
TATAATATACGCTATCCTTC+AGG 0.395137 2_3:+53053279 Msa0298850:CDS
TTTGGCTCCATCTTGACATT+CGG 0.395632 2_3:+53051452 Msa0298850:CDS
ATACCTGATTTAAGGTAGTC+AGG 0.404425 2_3:-53051995 None:intergenic
TTGTTATGTTCCCCTATCAA+TGG 0.404861 2_3:-53050803 None:intergenic
CTCATGAGTTGGAGCTCCTA+TGG 0.410624 2_3:+53054212 Msa0298850:CDS
ACTTGCCGTAAACGGCATAT+TGG 0.414060 2_3:+53053705 Msa0298850:CDS
TTATTACTTACCAGCCATCT+AGG 0.415487 2_3:-53052381 None:intergenic
ATAGGATTTCCTTCTGCAAT+GGG 0.419007 2_3:+53053253 Msa0298850:intron
TTCCTAATAGCTTCTTGAGT+TGG 0.419270 2_3:-53051096 None:intergenic
CTCTCAACTTTCTCATGAGT+TGG 0.427344 2_3:+53054201 Msa0298850:CDS
TGCCCTGACTACCTTAAATC+AGG 0.427867 2_3:+53051992 Msa0298850:CDS
TGGCTGCCTGTAATTGTTCA+AGG 0.428537 2_3:-53054461 None:intergenic
ATGCCGTTTACGGCAAGTGC+TGG 0.431492 2_3:-53053700 None:intergenic
TGAAACAAATCCAGCACCTT+CGG 0.432421 2_3:-53052739 None:intergenic
TCATTCCAGAGTCTCCTAGA+TGG 0.435503 2_3:+53052367 Msa0298850:CDS
TGTGTACAATCTTAGTTCCT+TGG 0.436869 2_3:-53050713 None:intergenic
TCTGGATCAGGATTGGTTGC+AGG 0.437259 2_3:+53053533 Msa0298850:CDS
GCATAGCAACATTAGTGAAC+TGG 0.437404 2_3:+53054153 Msa0298850:CDS
CTTCCAACGAAATCGGCAAT+AGG 0.438485 2_3:-53051503 None:intergenic
CTTCATGAGTAGATTGGAGT+AGG 0.442194 2_3:+53052976 Msa0298850:intron
ATATTTCCAGTCAATATTAA+GGG 0.442648 2_3:+53054119 Msa0298850:CDS
GCCGATTTCGTTGGAAGGAA+GGG 0.450130 2_3:+53051509 Msa0298850:CDS
GTCAATATTAAGGGGCAGGC+AGG 0.454849 2_3:+53054128 Msa0298850:CDS
ACAAATTATAATCAAAATCA+TGG 0.458705 2_3:-53054623 None:intergenic
TGAAACCACATGTTAAATCA+AGG 0.459071 2_3:-53054733 None:intergenic
GCAACATTAGTGAACTGGTT+TGG 0.459624 2_3:+53054158 Msa0298850:CDS
CATGAGAAAGTTGAGAGTAT+AGG 0.461407 2_3:-53054195 None:intergenic
TTGACATTCGGTGCGATGAT+TGG 0.461994 2_3:+53051464 Msa0298850:CDS
ATCAAGGAGGGAATTCGTAG+GGG 0.462019 2_3:-53054717 None:intergenic
TTTGCGATTTGTGTAGTGTT+TGG 0.466010 2_3:+53054499 Msa0298850:three_prime_UTR
TCCAGTCAATATTAAGGGGC+AGG 0.473585 2_3:+53054124 Msa0298850:CDS
GCTCGCAAAACCGAAGGTGC+TGG 0.477284 2_3:+53052729 Msa0298850:intron
CATAGGATTTCCTTCTGCAA+TGG 0.490067 2_3:+53053252 Msa0298850:intron
GAATAAGAGAACCATTGATA+GGG 0.493005 2_3:+53050792 Msa0298850:CDS
GCCTGCCCCTTAATATTGAC+TGG 0.493013 2_3:-53054125 None:intergenic
GATGGATATCAAAGAGGATG+TGG 0.494729 2_3:+53050751 Msa0298850:CDS
GTACAATCTTAGTTCCTTGG+TGG 0.500453 2_3:-53050710 None:intergenic
CTTTGCTTGCATGGACAAGT+TGG 0.500961 2_3:-53050826 None:intergenic
TGCCGATTTCGTTGGAAGGA+AGG 0.501963 2_3:+53051508 Msa0298850:CDS
GCGATTTGTGTAGTGTTTGG+TGG 0.502161 2_3:+53054502 Msa0298850:three_prime_UTR
GATATTGGGAGACTAGCAAC+AGG 0.513165 2_3:+53051805 Msa0298850:CDS
TCAAGGAGGGAATTCGTAGG+GGG 0.513902 2_3:-53054716 None:intergenic
CCGATTTCGTTGGAAGGAAG+GGG 0.516147 2_3:+53051510 Msa0298850:CDS
TTAGGTATCTGGATCAGGAT+TGG 0.517795 2_3:+53053526 Msa0298850:intron
TCCCTCCTTGATTTAACATG+TGG 0.518704 2_3:+53054728 Msa0298850:three_prime_UTR
TCCAGAGTCTCCTAGATGGC+TGG 0.523060 2_3:+53052371 Msa0298850:CDS
TGCCGTTTACGGCAAGTGCT+GGG 0.527568 2_3:-53053699 None:intergenic
GGACCTATTGCCGATTTCGT+TGG 0.533309 2_3:+53051500 Msa0298850:CDS
TTCATGAGTAGATTGGAGTA+GGG 0.538540 2_3:+53052977 Msa0298850:intron
ACCAGCCATCTAGGAGACTC+TGG 0.543206 2_3:-53052372 None:intergenic
ACACAGGTTTAGAATGAAGA+TGG 0.545944 2_3:+53050733 Msa0298850:exon
GATGATGATCTTTGCTTGCA+TGG 0.548086 2_3:-53050835 None:intergenic
CTAGCAACAGGATATGGAAT+GGG 0.555237 2_3:+53051817 Msa0298850:CDS
TAGTGAACTGGTTTGGTGCA+TGG 0.558886 2_3:+53054165 Msa0298850:CDS
GCAAAGATCATCATCATCCA+TGG 0.563043 2_3:+53050843 Msa0298850:CDS
TACCTGATTTAAGGTAGTCA+GGG 0.563340 2_3:-53051994 None:intergenic
CAAGGAGGGAATTCGTAGGG+GGG 0.563710 2_3:-53054715 None:intergenic
ACAGGTTCATGATGTGGCTG+TGG 0.570506 2_3:+53053669 Msa0298850:intron
CATCGCACCGAATGTCAAGA+TGG 0.572597 2_3:-53051459 None:intergenic
AGCAGTTGCATTCTATCTCA+AGG 0.573615 2_3:+53053568 Msa0298850:CDS
AGATGCTGATATATCTGAAG+AGG 0.576120 2_3:+53052551 Msa0298850:CDS
ACCCAGAAGCAACACAGCAG+TGG 0.576369 2_3:-53052337 None:intergenic
CCCAGAAGCAACACAGCAGT+GGG 0.578353 2_3:-53052336 None:intergenic
AATCAAGGAGGGAATTCGTA+GGG 0.578579 2_3:-53054718 None:intergenic
ACTAGCAACAGGATATGGAA+TGG 0.580078 2_3:+53051816 Msa0298850:CDS
AAGGAGGGAATTCGTAGGGG+GGG 0.587854 2_3:-53054714 None:intergenic
TATTTCCAGTCAATATTAAG+GGG 0.587860 2_3:+53054120 Msa0298850:CDS
ATTATACTCCCCATTGCAGA+AGG 0.590375 2_3:-53053262 None:intergenic
AACTCAAGAAGCTATTAGGA+AGG 0.591182 2_3:+53051098 Msa0298850:CDS
AATGAAGATGGATATCAAAG+AGG 0.592160 2_3:+53050745 Msa0298850:exon
AAATCAAGGAGGGAATTCGT+AGG 0.594919 2_3:-53054719 None:intergenic
GTGGTGAAGTAAACCATCCA+TGG 0.596687 2_3:-53050860 None:intergenic
AGATGGAGCCAAACAAGGAG+TGG 0.598168 2_3:-53051442 None:intergenic
CTATTGCCGATTTCGTTGGA+AGG 0.599087 2_3:+53051504 Msa0298850:CDS
GGTGGAAATTGGAACAACAA+AGG 0.604202 2_3:+53054520 Msa0298850:three_prime_UTR
CGATTTCGTTGGAAGGAAGG+GGG 0.607394 2_3:+53051511 Msa0298850:CDS
ATGGTTTGCCAGCAATTAGG+GGG 0.608563 2_3:+53053003 Msa0298850:CDS
ACAGGCAGCCATTATCTCGT+AGG 0.611757 2_3:+53054473 Msa0298850:CDS
AGATCATCATCATCCATGGA+TGG 0.612235 2_3:+53050847 Msa0298850:CDS
CAGGTTCATGATGTGGCTGT+GGG 0.612693 2_3:+53053670 Msa0298850:intron
ACCACATGTTAAATCAAGGA+GGG 0.613521 2_3:-53054729 None:intergenic
AATAAGAGAACCATTGATAG+GGG 0.617887 2_3:+53050793 Msa0298850:CDS
TGTCAAGATGGAGCCAAACA+AGG 0.620309 2_3:-53051447 None:intergenic
TAATGAATGCAGGCAAAGCG+AGG 0.623838 2_3:+53052483 Msa0298850:intron
ATCAAAGAGGATGTGGAAGG+TGG 0.623969 2_3:+53050758 Msa0298850:CDS
TATGGTTTGCCAGCAATTAG+GGG 0.634878 2_3:+53053002 Msa0298850:CDS
CTCCCAATATCCAAAGACAC+TGG 0.634926 2_3:-53051793 None:intergenic
AGAACAGCTCGCAAAACCGA+AGG 0.635960 2_3:+53052723 Msa0298850:intron
GCAGCATTACAAATACTTCG+CGG 0.650248 2_3:+53052525 Msa0298850:CDS
GATATCAAAGAGGATGTGGA+AGG 0.653697 2_3:+53050755 Msa0298850:CDS
GTAGTAGTCCCAGAAACCAA+AGG 0.657750 2_3:+53054432 Msa0298850:CDS
TGAGTAACAGGTTCATGATG+TGG 0.664870 2_3:+53053663 Msa0298850:intron
AACCACATGTTAAATCAAGG+AGG 0.666340 2_3:-53054730 None:intergenic
ATGATTGGTGCAATAACAAG+TGG 0.674569 2_3:+53051479 Msa0298850:CDS
ACCACAAACTGCAATGAATG+TGG 0.678039 2_3:-53050879 None:intergenic
TAGGATTTCCTTCTGCAATG+GGG 0.687197 2_3:+53053254 Msa0298850:intron
GGAACTAAGATTGTACACAC+AGG 0.703275 2_3:+53050717 Msa0298850:five_prime_UTR
GATTGCAGCGTAAAGAACGA+AGG 0.710685 2_3:-53054382 None:intergenic
CATAGTCATCAATACCACCA+AGG 0.718268 2_3:+53050696 Msa0298850:five_prime_UTR
ATTGCGCCAAAAGAACTCTG+AGG 0.734253 2_3:+53051969 Msa0298850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCAATTTTACATAAATATT+AGG - chr2_3:53053634-53053653 None:intergenic 10.0%
!!! ATTTTGATTATAATTTGTAT+TGG + chr2_3:53054628-53054647 Msa0298850:three_prime_UTR 10.0%
!!! GATAAATTTTTCAACAAAAT+TGG - chr2_3:53051370-53051389 None:intergenic 15.0%
!!! ATTTTGTTGAAAAATTTATC+TGG + chr2_3:53051370-53051389 Msa0298850:intron 15.0%
!! AATAAAGATAATCAACAATT+TGG - chr2_3:53051579-53051598 None:intergenic 15.0%
!! AAAAAAGTTCAGATATTATT+TGG - chr2_3:53051755-53051774 None:intergenic 15.0%
!!! TTTATTTTGTTTTGGTTTAT+TGG + chr2_3:53053218-53053237 Msa0298850:intron 15.0%
!! ATTAACCAATAATTAATTCT+TGG + chr2_3:53053315-53053334 Msa0298850:intron 15.0%
!!! ATTTTCCAAGAATTAATTAT+TGG - chr2_3:53053323-53053342 None:intergenic 15.0%
!! AAATAAAAATAAGTTGTTTG+TGG - chr2_3:53053449-53053468 None:intergenic 15.0%
!!! TACTTTTATTTATGATTTTC+AGG + chr2_3:53054357-53054376 Msa0298850:intron 15.0%
!! ACAAATTATAATCAAAATCA+TGG - chr2_3:53054626-53054645 None:intergenic 15.0%
!!! TGTTGTTATTTTGTTTTTTC+AGG + chr2_3:53051059-53051078 Msa0298850:intron 20.0%
!!! ATCAATTTTTCAATCATTAC+TGG - chr2_3:53051152-53051171 None:intergenic 20.0%
!!! AAAATGATGCTAGTTATTTT+TGG + chr2_3:53051544-53051563 Msa0298850:intron 20.0%
!!! TGTTGATTATCTTTATTATG+TGG + chr2_3:53051582-53051601 Msa0298850:intron 20.0%
!! GTTGATTATCTTTATTATGT+GGG + chr2_3:53051583-53051602 Msa0298850:intron 20.0%
!! TTGATTATCTTTATTATGTG+GGG + chr2_3:53051584-53051603 Msa0298850:intron 20.0%
!!! TATTCATAAAAAGTTTGTTC+TGG + chr2_3:53051607-53051626 Msa0298850:intron 20.0%
!!! TTTTTTTCTTCCCTTTAATA+GGG + chr2_3:53051769-53051788 Msa0298850:intron 20.0%
!! TTTGATGTGTTACATATATA+AGG + chr2_3:53051926-53051945 Msa0298850:intron 20.0%
!! AGAAAAATATACCTGATTTA+AGG - chr2_3:53052006-53052025 None:intergenic 20.0%
!! CATCTTAATTGCATTATATT+TGG - chr2_3:53052663-53052682 None:intergenic 20.0%
!! CAAATATAATGCAATTAAGA+TGG + chr2_3:53052661-53052680 Msa0298850:intron 20.0%
!!! CTCAGATTTTTATTTTGTTT+TGG + chr2_3:53053210-53053229 Msa0298850:intron 20.0%
!! ATTTAAAAATAAGAACCTGA+AGG - chr2_3:53053297-53053316 None:intergenic 20.0%
!! AATAAAAATCATGATTGAGT+TGG - chr2_3:53053368-53053387 None:intergenic 20.0%
!!! ATACTCTTATTGTTATTTCT+TGG + chr2_3:53053497-53053516 Msa0298850:intron 20.0%
!! TTATTGATATTAAAAACCCA+TGG - chr2_3:53053872-53053891 None:intergenic 20.0%
!! ATATTTCCAGTCAATATTAA+GGG + chr2_3:53054119-53054138 Msa0298850:CDS 20.0%
!! AATGTATTAGTATTTACCAT+AGG - chr2_3:53054231-53054250 None:intergenic 20.0%
!! GGAAATGCATTATAATATTA+AGG - chr2_3:53054696-53054715 None:intergenic 20.0%
!!! TTTTGGTTAATCTAATCCTA+AGG - chr2_3:53051188-53051207 None:intergenic 25.0%
! GATTAGATTAACCAAAACAT+AGG + chr2_3:53051191-53051210 Msa0298850:intron 25.0%
!! GAAATACAAACAATGTTTTG+AGG + chr2_3:53051279-53051298 Msa0298850:intron 25.0%
!! TTTGTATAACTTGTACTTGA+TGG + chr2_3:53051336-53051355 Msa0298850:intron 25.0%
!!! GTAATTTTCTTTGCTTTTTG+CGG + chr2_3:53051400-53051419 Msa0298850:intron 25.0%
!!! TTGTTCTGGAATATATATGT+AGG + chr2_3:53051621-53051640 Msa0298850:intron 25.0%
!! CGAAAAAATCACATAAGTTT+TGG - chr2_3:53051719-53051738 None:intergenic 25.0%
!!! TATTATTTGGAGTATCGTTA+TGG - chr2_3:53051742-53051761 None:intergenic 25.0%
!!! CTTTTTTTCTTCCCTTTAAT+AGG + chr2_3:53051768-53051787 Msa0298850:intron 25.0%
! AATAGAACAAATATGAGAAG+TGG - chr2_3:53052907-53052926 None:intergenic 25.0%
!! AGGAAGATGAAAATTTTGAA+TGG - chr2_3:53052950-53052969 None:intergenic 25.0%
! ATCTACTCATGAAGAAAAAT+AGG - chr2_3:53052970-53052989 None:intergenic 25.0%
!! ATTTTTCTTCATGAGTAGAT+TGG + chr2_3:53052970-53052989 Msa0298850:intron 25.0%
!!! TATTGGTGTATTTTTGTCAT+AGG + chr2_3:53053235-53053254 Msa0298850:intron 25.0%
!!! GTTATTTCTTGGTTTCAATT+AGG + chr2_3:53053508-53053527 Msa0298850:intron 25.0%
! GATATTTCCAGTCAATATTA+AGG + chr2_3:53054118-53054137 Msa0298850:intron 25.0%
! TATTTCCAGTCAATATTAAG+GGG + chr2_3:53054120-53054139 Msa0298850:CDS 25.0%
! CGATTATCGTATAATAATTC+TGG - chr2_3:53054296-53054315 None:intergenic 25.0%
TATGATTGTGACTGTGTTAT+AGG - chr2_3:53050680-53050699 None:intergenic 30.0%
AATGAAGATGGATATCAAAG+AGG + chr2_3:53050745-53050764 Msa0298850:exon 30.0%
GAATAAGAGAACCATTGATA+GGG + chr2_3:53050792-53050811 Msa0298850:CDS 30.0%
AATAAGAGAACCATTGATAG+GGG + chr2_3:53050793-53050812 Msa0298850:CDS 30.0%
! GTATGTGTTTAATTTCTCTG+TGG + chr2_3:53051033-53051052 Msa0298850:intron 30.0%
!!! GTTATTTTGTTTTTTCAGGC+TGG + chr2_3:53051063-53051082 Msa0298850:intron 30.0%
GATTTAATTGCATGTACCTT+AGG + chr2_3:53051169-53051188 Msa0298850:intron 30.0%
! TCAGTCTATTTCCTATGTTT+TGG - chr2_3:53051205-53051224 None:intergenic 30.0%
CATAGGAAATAGACTGAAAA+TGG + chr2_3:53051208-53051227 Msa0298850:intron 30.0%
GTAAATATACAGTTGAACCT+TGG + chr2_3:53051230-53051249 Msa0298850:intron 30.0%
!!! GCTTGTTATTTACTTTTCTG+AGG + chr2_3:53051681-53051700 Msa0298850:CDS 30.0%
!!! AATGGGAGTTTTTTCATTTG+TGG + chr2_3:53051834-53051853 Msa0298850:CDS 30.0%
! GTATCAGTGTCATTCATAAT+TGG + chr2_3:53052143-53052162 Msa0298850:intron 30.0%
TAATTGGAACAGTACTTTCA+TGG + chr2_3:53052159-53052178 Msa0298850:intron 30.0%
TCATATGATCTTCTTCTTAG+TGG - chr2_3:53052235-53052254 None:intergenic 30.0%
AAATAAGTGTTTGCAATTGC+AGG + chr2_3:53052306-53052325 Msa0298850:intron 30.0%
ATTTAGATGAAAACATGCAG+TGG + chr2_3:53052420-53052439 Msa0298850:intron 30.0%
! TTCATTATTTGGTTGGTACT+TGG - chr2_3:53052470-53052489 None:intergenic 30.0%
ACTAGTTATATAATCCTGTG+TGG - chr2_3:53052701-53052720 None:intergenic 30.0%
AAATGAACTGATATTCAGAG+TGG - chr2_3:53052806-53052825 None:intergenic 30.0%
! ATTTTCTTCAAGAATGTGTG+TGG + chr2_3:53052822-53052841 Msa0298850:intron 30.0%
AGAGGTGGAAAATAAAGTAT+TGG + chr2_3:53052860-53052879 Msa0298850:intron 30.0%
!! AGTAGTATTTTTGATCTAGC+AGG + chr2_3:53053054-53053073 Msa0298850:CDS 30.0%
! AAGTATGATGGTGTTAAACT+TGG - chr2_3:53053110-53053129 None:intergenic 30.0%
! TGATATGCAAGAAAGTATGA+TGG - chr2_3:53053122-53053141 None:intergenic 30.0%
TATAATATACGCTATCCTTC+AGG + chr2_3:53053279-53053298 Msa0298850:CDS 30.0%
!! AATCATGATTGAGTTGGATT+CGG - chr2_3:53053362-53053381 None:intergenic 30.0%
!! TAAGAGTATCATACATGAAG+CGG - chr2_3:53053486-53053505 None:intergenic 30.0%
AAGCTGTAAAGTTCTTAGTT+GGG - chr2_3:53053599-53053618 None:intergenic 30.0%
! CTCTTTCATTTGCTGATATT+AGG + chr2_3:53053808-53053827 Msa0298850:intron 30.0%
! TACATAGGATCGTTTTCAAT+AGG + chr2_3:53053833-53053852 Msa0298850:intron 30.0%
AAAAGAAAAACAGTTCCAGT+CGG - chr2_3:53053968-53053987 None:intergenic 30.0%
! TTTTCTTTTTCTTTGACCTG+AGG + chr2_3:53053979-53053998 Msa0298850:intron 30.0%
! ATTGGTTTCTTGAACCAAAT+GGG - chr2_3:53054323-53054342 None:intergenic 30.0%
!! TATTGGTTTCTTGAACCAAA+TGG - chr2_3:53054324-53054343 None:intergenic 30.0%
AGTAAACTTGCATGTTCTAT+TGG - chr2_3:53054341-53054360 None:intergenic 30.0%
TAAAAGCCTTGAACAATTAC+AGG + chr2_3:53054455-53054474 Msa0298850:CDS 30.0%
CCAACAGACTATAAAAAACA+AGG - chr2_3:53054574-53054593 None:intergenic 30.0%
!!! CCTTGTTTTTTATAGTCTGT+TGG + chr2_3:53054571-53054590 Msa0298850:three_prime_UTR 30.0%
TGAAACCACATGTTAAATCA+AGG - chr2_3:53054736-53054755 None:intergenic 30.0%
ACATGTGGTTTCATAAAGAA+TGG + chr2_3:53054743-53054762 Msa0298850:three_prime_UTR 30.0%
TGTGTACAATCTTAGTTCCT+TGG - chr2_3:53050716-53050735 None:intergenic 35.0%
! ACACAGGTTTAGAATGAAGA+TGG + chr2_3:53050733-53050752 Msa0298850:exon 35.0%
GGAATAAGAGAACCATTGAT+AGG + chr2_3:53050791-53050810 Msa0298850:CDS 35.0%
TTGTTATGTTCCCCTATCAA+TGG - chr2_3:53050806-53050825 None:intergenic 35.0%
GTTTGTGGTTCTTATGAGTT+TGG + chr2_3:53050893-53050912 Msa0298850:CDS 35.0%
TTCCTAATAGCTTCTTGAGT+TGG - chr2_3:53051099-53051118 None:intergenic 35.0%
AACTCAAGAAGCTATTAGGA+AGG + chr2_3:53051098-53051117 Msa0298850:CDS 35.0%
ATGATTGGTGCAATAACAAG+TGG + chr2_3:53051479-53051498 Msa0298850:CDS 35.0%
!! TCAAGTGCTTTTTGTATTGC+AGG + chr2_3:53051655-53051674 Msa0298850:CDS 35.0%
!! CAAGTGCTTTTTGTATTGCA+GGG + chr2_3:53051656-53051675 Msa0298850:CDS 35.0%
!!! GGAGTTTTTTCATTTGTGGT+TGG + chr2_3:53051838-53051857 Msa0298850:CDS 35.0%
! TACCTGATTTAAGGTAGTCA+GGG - chr2_3:53051997-53052016 None:intergenic 35.0%
! ATACCTGATTTAAGGTAGTC+AGG - chr2_3:53051998-53052017 None:intergenic 35.0%
TTATTACTTACCAGCCATCT+AGG - chr2_3:53052384-53052403 None:intergenic 35.0%
ACCAACCAAATAATGAATGC+AGG + chr2_3:53052473-53052492 Msa0298850:intron 35.0%
CTTTGCCTGCATTCATTATT+TGG - chr2_3:53052481-53052500 None:intergenic 35.0%
AGATGCTGATATATCTGAAG+AGG + chr2_3:53052551-53052570 Msa0298850:CDS 35.0%
GTCTTTATTATTGTGCCTGA+AGG - chr2_3:53052634-53052653 None:intergenic 35.0%
TTCATGAGTAGATTGGAGTA+GGG + chr2_3:53052977-53052996 Msa0298850:intron 35.0%
! TTATGGTTTGCCAGCAATTA+GGG + chr2_3:53053001-53053020 Msa0298850:CDS 35.0%
ATAGGATTTCCTTCTGCAAT+GGG + chr2_3:53053253-53053272 Msa0298850:intron 35.0%
!! ATCATGATTGAGTTGGATTC+GGG - chr2_3:53053361-53053380 None:intergenic 35.0%
! TTTTCACAGATTGTGATCAC+AGG + chr2_3:53053386-53053405 Msa0298850:intron 35.0%
AATAAGTTGTTTGTGGCTTC+CGG - chr2_3:53053442-53053461 None:intergenic 35.0%
CTTGGTTTCAATTAGGTATC+TGG + chr2_3:53053515-53053534 Msa0298850:intron 35.0%
TTCAATTAGGTATCTGGATC+AGG + chr2_3:53053521-53053540 Msa0298850:intron 35.0%
GAAGCTGTAAAGTTCTTAGT+TGG - chr2_3:53053600-53053619 None:intergenic 35.0%
AATGAAAGAGCAGTGTTACA+TGG - chr2_3:53053798-53053817 None:intergenic 35.0%
TGCTGATATTAGGTCTACAT+AGG + chr2_3:53053818-53053837 Msa0298850:intron 35.0%
! AGGATCGTTTTCAATAGGAA+TGG + chr2_3:53053838-53053857 Msa0298850:intron 35.0%
! GGATCGTTTTCAATAGGAAT+GGG + chr2_3:53053839-53053858 Msa0298850:intron 35.0%
!!! GGGATCATTGATCTTGTTTT+TGG - chr2_3:53053946-53053965 None:intergenic 35.0%
AAAGAAAAACAGTTCCAGTC+GGG - chr2_3:53053967-53053986 None:intergenic 35.0%
TAAATCAACTTCGAATTCGC+CGG + chr2_3:53054029-53054048 Msa0298850:intron 35.0%
! AAGAGGACACATTTTGTAAC+CGG - chr2_3:53054051-53054070 None:intergenic 35.0%
GTGTCCTCTTAGTAAAAAGA+CGG + chr2_3:53054061-53054080 Msa0298850:intron 35.0%
! TATGCCGTCTTTTTACTAAG+AGG - chr2_3:53054068-53054087 None:intergenic 35.0%
CTGGAAATATCTGCAACATA+AGG - chr2_3:53054109-53054128 None:intergenic 35.0%
! CATGAGAAAGTTGAGAGTAT+AGG - chr2_3:53054198-53054217 None:intergenic 35.0%
! ACGATAATCGTTTTCCCATT+TGG + chr2_3:53054306-53054325 Msa0298850:intron 35.0%
! TTGTTCAAGGCTTTTACCTT+TGG - chr2_3:53054451-53054470 None:intergenic 35.0%
TTTGCGATTTGTGTAGTGTT+TGG + chr2_3:53054499-53054518 Msa0298850:three_prime_UTR 35.0%
ACCACATGTTAAATCAAGGA+GGG - chr2_3:53054732-53054751 None:intergenic 35.0%
AACCACATGTTAAATCAAGG+AGG - chr2_3:53054733-53054752 None:intergenic 35.0%
CATAGTCATCAATACCACCA+AGG + chr2_3:53050696-53050715 Msa0298850:five_prime_UTR 40.0%
GTACAATCTTAGTTCCTTGG+TGG - chr2_3:53050713-53050732 None:intergenic 40.0%
GGAACTAAGATTGTACACAC+AGG + chr2_3:53050717-53050736 Msa0298850:five_prime_UTR 40.0%
GATGGATATCAAAGAGGATG+TGG + chr2_3:53050751-53050770 Msa0298850:CDS 40.0%
GATATCAAAGAGGATGTGGA+AGG + chr2_3:53050755-53050774 Msa0298850:CDS 40.0%
GATGATGATCTTTGCTTGCA+TGG - chr2_3:53050838-53050857 None:intergenic 40.0%
GCAAAGATCATCATCATCCA+TGG + chr2_3:53050843-53050862 Msa0298850:CDS 40.0%
AGATCATCATCATCCATGGA+TGG + chr2_3:53050847-53050866 Msa0298850:CDS 40.0%
ACCACATTCATTGCAGTTTG+TGG + chr2_3:53050878-53050897 Msa0298850:CDS 40.0%
ACCACAAACTGCAATGAATG+TGG - chr2_3:53050882-53050901 None:intergenic 40.0%
CTCCAACTCAAGAAGCTATT+AGG + chr2_3:53051094-53051113 Msa0298850:CDS 40.0%
GAAGGATCTTAGTCTGTCTT+TGG + chr2_3:53051116-53051135 Msa0298850:intron 40.0%
ATTGCTTATACTAGCAGCCA+AGG - chr2_3:53051250-53051269 None:intergenic 40.0%
! TTTGGCTCCATCTTGACATT+CGG + chr2_3:53051452-53051471 Msa0298850:CDS 40.0%
AGACACTGGACCCTATTAAA+GGG - chr2_3:53051782-53051801 None:intergenic 40.0%
AAGACACTGGACCCTATTAA+AGG - chr2_3:53051783-53051802 None:intergenic 40.0%
TTAATAGGGTCCAGTGTCTT+TGG + chr2_3:53051783-53051802 Msa0298850:intron 40.0%
GTCCAGTGTCTTTGGATATT+GGG + chr2_3:53051791-53051810 Msa0298850:CDS 40.0%
ACTAGCAACAGGATATGGAA+TGG + chr2_3:53051816-53051835 Msa0298850:CDS 40.0%
CTAGCAACAGGATATGGAAT+GGG + chr2_3:53051817-53051836 Msa0298850:CDS 40.0%
TTGGAACAGTACTTTCATGG+AGG + chr2_3:53052162-53052181 Msa0298850:intron 40.0%
TGGAACAGTACTTTCATGGA+GGG + chr2_3:53052163-53052182 Msa0298850:intron 40.0%
! TGGAGGGCTTTAGCTATAAT+CGG + chr2_3:53052179-53052198 Msa0298850:intron 40.0%
GCCTGCATTCATTATTTGGT+TGG - chr2_3:53052477-53052496 None:intergenic 40.0%
! AGGACGCACAAAAGATTTTG+TGG + chr2_3:53052503-53052522 Msa0298850:CDS 40.0%
GCAGCATTACAAATACTTCG+CGG + chr2_3:53052525-53052544 Msa0298850:CDS 40.0%
TGAAGAGGCAGAAGAAATTC+AGG + chr2_3:53052566-53052585 Msa0298850:CDS 40.0%
AGGTAACTGTGTGTTAGTGT+TGG + chr2_3:53052586-53052605 Msa0298850:intron 40.0%
TATTAAGTGACCTTGCCTTC+AGG + chr2_3:53052616-53052635 Msa0298850:intron 40.0%
TATTATTGTGCCTGAAGGCA+AGG - chr2_3:53052629-53052648 None:intergenic 40.0%
TGAAACAAATCCAGCACCTT+CGG - chr2_3:53052742-53052761 None:intergenic 40.0%
TTCTTCAAGAATGTGTGTGG+TGG + chr2_3:53052825-53052844 Msa0298850:intron 40.0%
CTTCATGAGTAGATTGGAGT+AGG + chr2_3:53052976-53052995 Msa0298850:intron 40.0%
! CTTATGGTTTGCCAGCAATT+AGG + chr2_3:53053000-53053019 Msa0298850:CDS 40.0%
! TATGGTTTGCCAGCAATTAG+GGG + chr2_3:53053002-53053021 Msa0298850:CDS 40.0%
CATAGGATTTCCTTCTGCAA+TGG + chr2_3:53053252-53053271 Msa0298850:intron 40.0%
TAGGATTTCCTTCTGCAATG+GGG + chr2_3:53053254-53053273 Msa0298850:intron 40.0%
ATTATACTCCCCATTGCAGA+AGG - chr2_3:53053265-53053284 None:intergenic 40.0%
!! TCATGATTGAGTTGGATTCG+GGG - chr2_3:53053360-53053379 None:intergenic 40.0%
TTAGGTATCTGGATCAGGAT+TGG + chr2_3:53053526-53053545 Msa0298850:intron 40.0%
AGCAGTTGCATTCTATCTCA+AGG + chr2_3:53053568-53053587 Msa0298850:CDS 40.0%
GATATCCTTGTGTGAGTAAC+AGG + chr2_3:53053651-53053670 Msa0298850:intron 40.0%
ATGAACCTGTTACTCACACA+AGG - chr2_3:53053659-53053678 None:intergenic 40.0%
TGAGTAACAGGTTCATGATG+TGG + chr2_3:53053663-53053682 Msa0298850:intron 40.0%
! CCATACCAATATGCCGTTTA+CGG - chr2_3:53053713-53053732 None:intergenic 40.0%
AAGAAAAACAGTTCCAGTCG+GGG - chr2_3:53053966-53053985 None:intergenic 40.0%
! TCTTTTTCTTTGACCTGAGG+AGG + chr2_3:53053982-53054001 Msa0298850:intron 40.0%
TGCATAAATAGATCCTCCTC+AGG - chr2_3:53053998-53054017 None:intergenic 40.0%
GCATAGCAACATTAGTGAAC+TGG + chr2_3:53054153-53054172 Msa0298850:CDS 40.0%
GCAACATTAGTGAACTGGTT+TGG + chr2_3:53054158-53054177 Msa0298850:CDS 40.0%
CTCTCAACTTTCTCATGAGT+TGG + chr2_3:53054201-53054220 Msa0298850:CDS 40.0%
!! GGCTTTTACCTTTGGTTTCT+GGG - chr2_3:53054443-53054462 None:intergenic 40.0%
!! AGGCTTTTACCTTTGGTTTC+TGG - chr2_3:53054444-53054463 None:intergenic 40.0%
GCAAACTTCCTACGAGATAA+TGG - chr2_3:53054484-53054503 None:intergenic 40.0%
! GTGTAGTGTTTGGTGGAAAT+TGG + chr2_3:53054509-53054528 Msa0298850:three_prime_UTR 40.0%
GGTGGAAATTGGAACAACAA+AGG + chr2_3:53054520-53054539 Msa0298850:three_prime_UTR 40.0%
AATCAAGGAGGGAATTCGTA+GGG - chr2_3:53054721-53054740 None:intergenic 40.0%
AAATCAAGGAGGGAATTCGT+AGG - chr2_3:53054722-53054741 None:intergenic 40.0%
TCCCTCCTTGATTTAACATG+TGG + chr2_3:53054728-53054747 Msa0298850:three_prime_UTR 40.0%
ATCAAAGAGGATGTGGAAGG+TGG + chr2_3:53050758-53050777 Msa0298850:CDS 45.0%
GTGGAAGGTGGCAAACAAAA+AGG + chr2_3:53050770-53050789 Msa0298850:CDS 45.0%
CTTTGCTTGCATGGACAAGT+TGG - chr2_3:53050829-53050848 None:intergenic 45.0%
GTGGTGAAGTAAACCATCCA+TGG - chr2_3:53050863-53050882 None:intergenic 45.0%
TCTTAGTCTGTCTTTGGCAG+AGG + chr2_3:53051122-53051141 Msa0298850:intron 45.0%
TGTCAAGATGGAGCCAAACA+AGG - chr2_3:53051450-53051469 None:intergenic 45.0%
!! TTGACATTCGGTGCGATGAT+TGG + chr2_3:53051464-53051483 Msa0298850:CDS 45.0%
CTTCCAACGAAATCGGCAAT+AGG - chr2_3:53051506-53051525 None:intergenic 45.0%
!! CTATTGCCGATTTCGTTGGA+AGG + chr2_3:53051504-53051523 Msa0298850:CDS 45.0%
!! GTGCTTTTTGTATTGCAGGG+TGG + chr2_3:53051659-53051678 Msa0298850:CDS 45.0%
GGTCCAGTGTCTTTGGATAT+TGG + chr2_3:53051790-53051809 Msa0298850:CDS 45.0%
CTCCCAATATCCAAAGACAC+TGG - chr2_3:53051796-53051815 None:intergenic 45.0%
GATATTGGGAGACTAGCAAC+AGG + chr2_3:53051805-53051824 Msa0298850:CDS 45.0%
ATTGCGCCAAAAGAACTCTG+AGG + chr2_3:53051969-53051988 Msa0298850:CDS 45.0%
TGCCCTGACTACCTTAAATC+AGG + chr2_3:53051992-53052011 Msa0298850:CDS 45.0%
TCATTCCAGAGTCTCCTAGA+TGG + chr2_3:53052367-53052386 Msa0298850:CDS 45.0%
TAATGAATGCAGGCAAAGCG+AGG + chr2_3:53052483-53052502 Msa0298850:intron 45.0%
!! AATGTGTGTGGTGGACTACT+AGG + chr2_3:53052834-53052853 Msa0298850:intron 45.0%
GTAGATTGGAGTAGGGCTTA+TGG + chr2_3:53052984-53053003 Msa0298850:intron 45.0%
! ATGGTTTGCCAGCAATTAGG+GGG + chr2_3:53053003-53053022 Msa0298850:CDS 45.0%
!! CATTGATTCCCCCTAATTGC+TGG - chr2_3:53053014-53053033 None:intergenic 45.0%
CAGCAATTAGGGGGAATCAA+TGG + chr2_3:53053012-53053031 Msa0298850:CDS 45.0%
!! CATGATTGAGTTGGATTCGG+GGG - chr2_3:53053359-53053378 None:intergenic 45.0%
CAGATTGTGATCACAGGTGT+TGG + chr2_3:53053392-53053411 Msa0298850:intron 45.0%
ACTTGCCGTAAACGGCATAT+TGG + chr2_3:53053705-53053724 Msa0298850:CDS 45.0%
CCGTAAACGGCATATTGGTA+TGG + chr2_3:53053710-53053729 Msa0298850:intron 45.0%
TAGGAATGGGAGCAATTCCA+TGG + chr2_3:53053852-53053871 Msa0298850:intron 45.0%
AGGAATGGGAGCAATTCCAT+GGG + chr2_3:53053853-53053872 Msa0298850:intron 45.0%
TCCAGTCAATATTAAGGGGC+AGG + chr2_3:53054124-53054143 Msa0298850:CDS 45.0%
!! TAGTGAACTGGTTTGGTGCA+TGG + chr2_3:53054165-53054184 Msa0298850:CDS 45.0%
GATTGCAGCGTAAAGAACGA+AGG - chr2_3:53054385-53054404 None:intergenic 45.0%
GTAGTAGTCCCAGAAACCAA+AGG + chr2_3:53054432-53054451 Msa0298850:CDS 45.0%
TGGCTGCCTGTAATTGTTCA+AGG - chr2_3:53054464-53054483 None:intergenic 45.0%
GCGATTTGTGTAGTGTTTGG+TGG + chr2_3:53054502-53054521 Msa0298850:three_prime_UTR 45.0%
ATCAAGGAGGGAATTCGTAG+GGG - chr2_3:53054720-53054739 None:intergenic 45.0%
GCAGTACTCCACTCCTTGTT+TGG + chr2_3:53051434-53051453 Msa0298850:intron 50.0%
AGATGGAGCCAAACAAGGAG+TGG - chr2_3:53051445-53051464 None:intergenic 50.0%
CATCGCACCGAATGTCAAGA+TGG - chr2_3:53051462-53051481 None:intergenic 50.0%
!! GGACCTATTGCCGATTTCGT+TGG + chr2_3:53051500-53051519 Msa0298850:CDS 50.0%
!! TGCCGATTTCGTTGGAAGGA+AGG + chr2_3:53051508-53051527 Msa0298850:CDS 50.0%
! GCCGATTTCGTTGGAAGGAA+GGG + chr2_3:53051509-53051528 Msa0298850:CDS 50.0%
CCCCTTCCTTCCAACGAAAT+CGG - chr2_3:53051513-53051532 None:intergenic 50.0%
CCGATTTCGTTGGAAGGAAG+GGG + chr2_3:53051510-53051529 Msa0298850:CDS 50.0%
CGATTTCGTTGGAAGGAAGG+GGG + chr2_3:53051511-53051530 Msa0298850:CDS 50.0%
GGAAGGAAGGGGGTAATTTC+AGG + chr2_3:53051521-53051540 Msa0298850:intron 50.0%
GGGAGACTAGCAACAGGATA+TGG + chr2_3:53051811-53051830 Msa0298850:CDS 50.0%
!! AGGGCACCTCAGAGTTCTTT+TGG - chr2_3:53051978-53051997 None:intergenic 50.0%
GCAACACAGCAGTGGGAATA+AGG - chr2_3:53052332-53052351 None:intergenic 50.0%
TGTATCTGTCACTGCCACAC+AGG + chr2_3:53052684-53052703 Msa0298850:intron 50.0%
AGAACAGCTCGCAAAACCGA+AGG + chr2_3:53052723-53052742 Msa0298850:intron 50.0%
!! TGGTGGACTACTAGGTTGAG+AGG + chr2_3:53052842-53052861 Msa0298850:intron 50.0%
! TGGACTACTAGGTTGAGAGG+TGG + chr2_3:53052845-53052864 Msa0298850:intron 50.0%
!! TCTGGATCAGGATTGGTTGC+AGG + chr2_3:53053533-53053552 Msa0298850:CDS 50.0%
ACAGGTTCATGATGTGGCTG+TGG + chr2_3:53053669-53053688 Msa0298850:intron 50.0%
CAGGTTCATGATGTGGCTGT+GGG + chr2_3:53053670-53053689 Msa0298850:intron 50.0%
CAAGATCAATGATCCCCGAC+TGG + chr2_3:53053950-53053969 Msa0298850:intron 50.0%
GCCTGCCCCTTAATATTGAC+TGG - chr2_3:53054128-53054147 None:intergenic 50.0%
GTCAATATTAAGGGGCAGGC+AGG + chr2_3:53054128-53054147 Msa0298850:CDS 50.0%
! CTCATGAGTTGGAGCTCCTA+TGG + chr2_3:53054212-53054231 Msa0298850:CDS 50.0%
ACAGGCAGCCATTATCTCGT+AGG + chr2_3:53054473-53054492 Msa0298850:CDS 50.0%
TCAAGGAGGGAATTCGTAGG+GGG - chr2_3:53054719-53054738 None:intergenic 50.0%
!! TCCCACTGCTGTGTTGCTTC+TGG + chr2_3:53052335-53052354 Msa0298850:CDS 55.0%
CCCAGAAGCAACACAGCAGT+GGG - chr2_3:53052339-53052358 None:intergenic 55.0%
!! CCCACTGCTGTGTTGCTTCT+GGG + chr2_3:53052336-53052355 Msa0298850:CDS 55.0%
ACCCAGAAGCAACACAGCAG+TGG - chr2_3:53052340-53052359 None:intergenic 55.0%
TCCAGAGTCTCCTAGATGGC+TGG + chr2_3:53052371-53052390 Msa0298850:CDS 55.0%
ACCAGCCATCTAGGAGACTC+TGG - chr2_3:53052375-53052394 None:intergenic 55.0%
GCAGCCCTCATAGATAGAGC+AGG + chr2_3:53053416-53053435 Msa0298850:intron 55.0%
CCGGCCTGCTCTATCTATGA+GGG - chr2_3:53053423-53053442 None:intergenic 55.0%
CCCTCATAGATAGAGCAGGC+CGG + chr2_3:53053420-53053439 Msa0298850:intron 55.0%
TCCGGCCTGCTCTATCTATG+AGG - chr2_3:53053424-53053443 None:intergenic 55.0%
GTCCCAGCACTTGCCGTAAA+CGG + chr2_3:53053697-53053716 Msa0298850:CDS 55.0%
! TGCCGTTTACGGCAAGTGCT+GGG - chr2_3:53053702-53053721 None:intergenic 55.0%
! ATGCCGTTTACGGCAAGTGC+TGG - chr2_3:53053703-53053722 None:intergenic 55.0%
AAGGAGGGAATTCGTAGGGG+GGG - chr2_3:53054717-53054736 None:intergenic 55.0%
CAAGGAGGGAATTCGTAGGG+GGG - chr2_3:53054718-53054737 None:intergenic 55.0%
! GCTCGCAAAACCGAAGGTGC+TGG + chr2_3:53052729-53052748 Msa0298850:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 53050637 53054775 53050637 ID=Msa0298850;Name=Msa0298850
chr2_3 mRNA 53050637 53054775 53050637 ID=Msa0298850-mRNA-1;Parent=Msa0298850;Name=Msa0298850-mRNA-1;_AED=0.24;_eAED=0.24;_QI=109|0.64|0.66|1|0.64|0.66|15|281|401
chr2_3 exon 53050637 53050922 53050637 ID=Msa0298850-mRNA-1:exon:16982;Parent=Msa0298850-mRNA-1
chr2_3 exon 53051081 53051126 53051081 ID=Msa0298850-mRNA-1:exon:16983;Parent=Msa0298850-mRNA-1
chr2_3 exon 53051438 53051532 53051438 ID=Msa0298850-mRNA-1:exon:16984;Parent=Msa0298850-mRNA-1
chr2_3 exon 53051643 53051702 53051643 ID=Msa0298850-mRNA-1:exon:16985;Parent=Msa0298850-mRNA-1
chr2_3 exon 53051790 53051859 53051790 ID=Msa0298850-mRNA-1:exon:16986;Parent=Msa0298850-mRNA-1
chr2_3 exon 53051948 53052013 53051948 ID=Msa0298850-mRNA-1:exon:16987;Parent=Msa0298850-mRNA-1
chr2_3 exon 53052328 53052392 53052328 ID=Msa0298850-mRNA-1:exon:16988;Parent=Msa0298850-mRNA-1
chr2_3 exon 53052495 53052587 53052495 ID=Msa0298850-mRNA-1:exon:16989;Parent=Msa0298850-mRNA-1
chr2_3 exon 53052730 53052786 53052730 ID=Msa0298850-mRNA-1:exon:16990;Parent=Msa0298850-mRNA-1
chr2_3 exon 53052988 53053075 53052988 ID=Msa0298850-mRNA-1:exon:16991;Parent=Msa0298850-mRNA-1
chr2_3 exon 53053257 53053300 53053257 ID=Msa0298850-mRNA-1:exon:16992;Parent=Msa0298850-mRNA-1
chr2_3 exon 53053530 53053589 53053530 ID=Msa0298850-mRNA-1:exon:16993;Parent=Msa0298850-mRNA-1
chr2_3 exon 53053673 53053726 53053673 ID=Msa0298850-mRNA-1:exon:16994;Parent=Msa0298850-mRNA-1
chr2_3 exon 53054119 53054233 53054119 ID=Msa0298850-mRNA-1:exon:16995;Parent=Msa0298850-mRNA-1
chr2_3 exon 53054379 53054775 53054379 ID=Msa0298850-mRNA-1:exon:16996;Parent=Msa0298850-mRNA-1
chr2_3 five_prime_UTR 53050637 53050745 53050637 ID=Msa0298850-mRNA-1:five_prime_utr;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53050746 53050922 53050746 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53051081 53051126 53051081 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53051438 53051532 53051438 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53051643 53051702 53051643 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53051790 53051859 53051790 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53051948 53052013 53051948 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53052328 53052392 53052328 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53052495 53052587 53052495 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53052730 53052786 53052730 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53052988 53053075 53052988 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53053257 53053300 53053257 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53053530 53053589 53053530 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53053673 53053726 53053673 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53054119 53054233 53054119 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 CDS 53054379 53054494 53054379 ID=Msa0298850-mRNA-1:cds;Parent=Msa0298850-mRNA-1
chr2_3 three_prime_UTR 53054495 53054775 53054495 ID=Msa0298850-mRNA-1:three_prime_utr;Parent=Msa0298850-mRNA-1
Gene Sequence

>Msa0298850

ATGAAGATGGATATCAAAGAGGATGTGGAAGGTGGCAAACAAAAAGGAATAAGAGAACCATTGATAGGGGAACATAACAACCAACTTGTCCATGCAAGCAAAGATCATCATCATCCATGGATGGTTTACTTCACCACATTCATTGCAGTTTGTGGTTCTTATGAGTTTGGTGCTTGTGCTGGATATTCATCTCCAACTCAAGAAGCTATTAGGAAGGATCTTATACTCCACTCCTTGTTTGGCTCCATCTTGACATTCGGTGCGATGATTGGTGCAATAACAAGTGGACCTATTGCCGATTTCGTTGGAAGGAAGGGGGCAATGAGAGTGTCAAGTGCTTTTTGTATTGCAGGGTGGCTTGTTATTTACTTTTCTGAGGGTCCAGTGTCTTTGGATATTGGGAGACTAGCAACAGGATATGGAATGGGAGTTTTTTCATTTGTGGTTGGTTACTGTGTTCGTAGCAGAAATTGCGCCAAAAGAACTCTGAGGTGCCCTGACTACCTTAAATCAGGCCTTATTCCCACTGCTGTGTTGCTTCTGGGTCTGTTTTTCATTCCAGAGTCTCCTAGATGGCTGGCAAAGCGAGGACGCACAAAAGATTTTGTGGCAGCATTACAAATACTTCGCGGTAAAGATGCTGATATATCTGAAGAGGCAGAAGAAATTCAGCTCGCAAAACCGAAGGTGCTGGATTTGTTTCAAAGAAGATATTTACGCTCACTCACAATTGGAGTAGGGCTTATGGTTTGCCAGCAATTAGGGGGAATCAATGGTGTTGTTTGCTTTTATACTAGTAGTATTTTTGATCTAGCAGGATTTCCTTCTGCAATGGGGAGTATAATATACGCTATCCTTCAGGTATCTGGATCAGGATTGGTTGCAGGATGCATATTTACAGCAGTTGCATTCTATCTCAAGGTTCATGATGTGGCTGTGGGAGCAGTCCCAGCACTTGCCGTAAACGGCATATTGATATTTCCAGTCAATATTAAGGGGCAGGCAGGAAGCATAGCAACATTAGTGAACTGGTTTGGTGCATGGTTATGCTCCTATACTCTCAACTTTCTCATGAGTTGGAGCTCCTATGGTACCTTCGTTCTTTACGCTGCAATCAATGCACTGTCTATATTATTTATAGTTGTAGTAGTCCCAGAAACCAAAGGTAAAAGCCTTGAACAATTACAGGCAGCCATTATCTCGTAG
Protein sequence

>Msa0298850

MKMDIKEDVEGGKQKGIREPLIGEHNNQLVHASKDHHHPWMVYFTTFIAVCGSYEFGACAGYSSPTQEAIRKDLILHSLFGSILTFGAMIGAITSGPIADFVGRKGAMRVSSAFCIAGWLVIYFSEGPVSLDIGRLATGYGMGVFSFVVGYCVRSRNCAKRTLRCPDYLKSGLIPTAVLLLGLFFIPESPRWLAKRGRTKDFVAALQILRGKDADISEEAEEIQLAKPKVLDLFQRRYLRSLTIGVGLMVCQQLGGINGVVCFYTSSIFDLAGFPSAMGSIIYAILQVSGSGLVAGCIFTAVAFYLKVHDVAVGAVPALAVNGILIFPVNIKGQAGSIATLVNWFGAWLCSYTLNFLMSWSSYGTFVLYAAINALSILFIVVVVPETKGKSLEQLQAAIIS*