AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0299420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299420 MtrunA17_Chr2g0323811 95.238 273 12 1 1 273 1 272 0.0 498
Msa0299420 MtrunA17_Chr5g0407481 93.056 144 10 0 130 273 1 144 4.61e-96 278
Msa0299420 MtrunA17_Chr2g0323801 81.250 144 21 1 130 273 1 138 4.19e-78 233
Msa0299420 MtrunA17_Chr4g0004251 34.574 188 106 3 78 261 77 251 3.99e-37 132
Msa0299420 MtrunA17_Chr5g0407491 66.667 90 17 2 2 84 1 84 7.49e-30 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299420 AT4G03320.1 43.662 284 141 7 6 272 1 282 1.42e-74 229
Msa0299420 AT1G04940.1 42.424 165 88 2 101 261 109 270 2.72e-46 156
Msa0299420 AT1G04945.3 44.595 148 75 2 101 244 109 253 8.41e-39 144

Find 97 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTCAAGCTTCTAAATTT+TGG 0.115139 2_3:-54361202 None:intergenic
CCCTATATTATACTGCTATT+TGG 0.188855 2_3:+54360692 Msa0299420:CDS
GAACACTTGGGATGTATTAT+TGG 0.195680 2_3:+54360850 Msa0299420:CDS
TTCTATTATATAAAGTTATA+AGG 0.207087 2_3:+54361113 Msa0299420:three_prime_UTR
TTTGGTATGACAAAATTTAT+AGG 0.251802 2_3:-54361253 None:intergenic
TCTATCAGATGTGCTCTTCT+TGG 0.261813 2_3:+54360912 Msa0299420:CDS
CTCTTGACTACTTCCTAAAA+TGG 0.273468 2_3:-54361011 None:intergenic
CTAGATCCTTCCACAGAAAA+AGG 0.285781 2_3:+54358911 Msa0299420:five_prime_UTR
CTTCATTCCCTACTTAATAT+AGG 0.308831 2_3:+54360975 Msa0299420:CDS
AAAGCTGATTCGCTAATAAC+AGG 0.313142 2_3:-54360951 None:intergenic
GGCAATCGGTTGACAGCTCC+TGG 0.313670 2_3:-54360660 None:intergenic
GGGATATTGAAATTCTGATA+TGG 0.316615 2_3:+54361134 Msa0299420:three_prime_UTR
GCCAAATAGCAGTATAATAT+AGG 0.316868 2_3:-54360693 None:intergenic
GAGTACTAGAACTGCTTCTT+CGG 0.324423 2_3:-54360449 None:intergenic
AAAGTTCTCCACCACCATTC+TGG 0.325031 2_3:-54360531 None:intergenic
TACATGGAATCTGAAGATAA+TGG 0.338807 2_3:-54360748 None:intergenic
GGATTTGAGCAGTAAGAATA+TGG 0.350629 2_3:-54361301 None:intergenic
CATCTGCAATGCTATTAGAT+AGG 0.350835 2_3:-54360565 None:intergenic
ACATGGAATCTGAAGATAAT+GGG 0.352918 2_3:-54360747 None:intergenic
TCTGGCTTCTCAGAATTTGC+AGG 0.362075 2_3:-54360513 None:intergenic
AACACTTGGGATGTATTATT+GGG 0.376072 2_3:+54360851 Msa0299420:CDS
TCTATTATATAAAGTTATAA+GGG 0.379118 2_3:+54361114 Msa0299420:three_prime_UTR
AGCTCTAGCAGGCAGTTCTC+TGG 0.391828 2_3:+54360005 Msa0299420:CDS
CCAAATAGCAGTATAATATA+GGG 0.392964 2_3:-54360692 None:intergenic
CTTCAGATTCCATGTAATGA+TGG 0.402408 2_3:+54360755 Msa0299420:CDS
AAATTGCTCTGCAGATTGTA+TGG 0.403836 2_3:+54360790 Msa0299420:CDS
GGTGGTTGAGGCGGCGGCTG+CGG 0.411828 2_3:-54359108 None:intergenic
ATACTGCTATTTGGCTATCA+TGG 0.434050 2_3:+54360701 Msa0299420:CDS
CTGTCAACCGATTGCCAACC+TGG 0.447918 2_3:+54360667 Msa0299420:CDS
GAGCTTCAATGCCCTCTTTG+AGG 0.449755 2_3:-54358889 None:intergenic
TAACGGAAGAATCGAGAGTC+AGG 0.462405 2_3:+54359047 Msa0299420:five_prime_UTR
TGTAATGATGGGAATGCTAC+TGG 0.470169 2_3:+54360767 Msa0299420:CDS
CATTCCCTACTTAATATAGG+AGG 0.477614 2_3:+54360978 Msa0299420:CDS
CATGCCACTGATACACTTCA+AGG 0.480381 2_3:+54360827 Msa0299420:CDS
GTAGGTGGTGGTTGAGGCGG+CGG 0.480996 2_3:-54359114 None:intergenic
GCATATATATACCATTGGTC+TGG 0.482252 2_3:+54360480 Msa0299420:CDS
GCATATTTGTAAACTATATG+TGG 0.483654 2_3:+54361033 Msa0299420:three_prime_UTR
CTATATTAAGTAGGGAATGA+AGG 0.486900 2_3:-54360974 None:intergenic
GAACTGCTTCTTCGGGAGGT+TGG 0.488847 2_3:-54360441 None:intergenic
ATACACTTCAAGGGAACACT+TGG 0.502149 2_3:+54360837 Msa0299420:CDS
GGAACCCTCCTATATTAAGT+AGG 0.502372 2_3:-54360983 None:intergenic
AAAGGTAATTACGGGAGAGA+AGG 0.503412 2_3:-54359155 None:intergenic
AGTACTAGAACTGCTTCTTC+GGG 0.508002 2_3:-54360448 None:intergenic
CTGGCTTCTCAGAATTTGCA+GGG 0.511599 2_3:-54360512 None:intergenic
GGCGGCTGCGGTTAATTGAC+CGG 0.514928 2_3:-54359096 None:intergenic
ATCTGCAATGCTATTAGATA+GGG 0.515006 2_3:-54360564 None:intergenic
TTATTGGGCTGGTGCGGCGT+TGG 0.520298 2_3:+54360866 Msa0299420:CDS
CTTGCCATCACAGCTCTAGC+AGG 0.524591 2_3:+54359994 Msa0299420:CDS
AGAAGCGTAGGTGGTGGTTG+AGG 0.524937 2_3:-54359120 None:intergenic
GCATTGCAGATGTCTGCTGC+TGG 0.526281 2_3:+54360576 Msa0299420:CDS
CTTGGGATGTATTATTGGGC+TGG 0.527106 2_3:+54360855 Msa0299420:CDS
GCTCTAGCAGGCAGTTCTCT+GGG 0.531889 2_3:+54360006 Msa0299420:CDS
CAAATTCTGAGAAGCCAGAA+TGG 0.534347 2_3:+54360517 Msa0299420:CDS
CACTAGCATATATATACCAT+TGG 0.536170 2_3:+54360475 Msa0299420:CDS
CTACTTCCTAAAATGGCCGT+TGG 0.536876 2_3:-54361004 None:intergenic
AGAAAGAAGACGATGATGGC+CGG 0.546522 2_3:+54359077 Msa0299420:exon
ATTCCCTACTTAATATAGGA+GGG 0.550198 2_3:+54360979 Msa0299420:CDS
AGCGTAGGTGGTGGTTGAGG+CGG 0.550230 2_3:-54359117 None:intergenic
TTCAGATTCCATGTAATGAT+GGG 0.550501 2_3:+54360756 Msa0299420:CDS
AAAGAGGTGGGTAAGGGTAA+CGG 0.555277 2_3:+54359030 Msa0299420:five_prime_UTR
CTATTTGGCTATCATGGTAG+TGG 0.557828 2_3:+54360707 Msa0299420:CDS
TAGGGAACCAGGTTGGCAAT+CGG 0.558555 2_3:-54360674 None:intergenic
GAACCCTCCTATATTAAGTA+GGG 0.566246 2_3:-54360982 None:intergenic
CTGAGAAGCCAGAATGGTGG+TGG 0.567799 2_3:+54360523 Msa0299420:CDS
AGGAGGACTTGTATGTCAGA+AGG 0.572437 2_3:-54358945 None:intergenic
AATTTGAATTTATCAAGCAA+AGG 0.581284 2_3:-54360612 None:intergenic
CATTGCAGATGTCTGCTGCT+GGG 0.582116 2_3:+54360577 Msa0299420:CDS
AAGAAGAAAGAAGACGATGA+TGG 0.582985 2_3:+54359073 Msa0299420:exon
AAGGCGGAGAAGAGAAGCGT+AGG 0.583667 2_3:-54359132 None:intergenic
GTAGCATTCCCATCATTACA+TGG 0.585539 2_3:-54360764 None:intergenic
ACTGCCTGCTAGAGCTGTGA+TGG 0.588886 2_3:-54359998 None:intergenic
TTAATATAGGAGGGTTCCAA+CGG 0.593163 2_3:+54360988 Msa0299420:CDS
GAGAAGAGAAGCGTAGGTGG+TGG 0.594095 2_3:-54359126 None:intergenic
GAATGGCACCGCCTCAAAGA+GGG 0.595895 2_3:+54358878 None:intergenic
GCAGTATAATATAGGGAACC+AGG 0.596497 2_3:-54360685 None:intergenic
GCGGAGAAGAGAAGCGTAGG+TGG 0.597528 2_3:-54359129 None:intergenic
ATGCCACTGATACACTTCAA+GGG 0.597572 2_3:+54360828 Msa0299420:CDS
CTTCAATGCCCTCTTTGAGG+CGG 0.601670 2_3:-54358886 None:intergenic
ACTAGAACTGCTTCTTCGGG+AGG 0.602107 2_3:-54360445 None:intergenic
AATTGCTCTGCAGATTGTAT+GGG 0.609591 2_3:+54360791 Msa0299420:CDS
CAATGGTATATATATGCTAG+TGG 0.609835 2_3:-54360474 None:intergenic
AGGTTGGAGCACTGAGTGAG+AGG 0.611111 2_3:-54360425 None:intergenic
GCAATCGGTTGACAGCTCCT+GGG 0.611407 2_3:-54360659 None:intergenic
CATGGAATCTGAAGATAATG+GGG 0.614103 2_3:-54360746 None:intergenic
CACTGAGTGAGAGGTGACCA+TGG 0.617796 2_3:-54360416 None:intergenic
GATGTATTATTGGGCTGGTG+CGG 0.620109 2_3:+54360860 Msa0299420:CDS
CGAATGGCACCGCCTCAAAG+AGG 0.635511 2_3:+54358877 None:intergenic
TATAATATAGGGAACCAGGT+TGG 0.639459 2_3:-54360681 None:intergenic
GTAATTACGGGAGAGAAGGA+AGG 0.642919 2_3:-54359151 None:intergenic
AAGCTGATTCGCTAATAACA+GGG 0.643323 2_3:-54360950 None:intergenic
GGATTCGAAAACCAGACCAA+TGG 0.654200 2_3:-54360491 None:intergenic
GTTCCCTTGAAGTGTATCAG+TGG 0.656964 2_3:-54360831 None:intergenic
TACACTTCAAGGGAACACTT+GGG 0.657791 2_3:+54360838 Msa0299420:CDS
CCATGGTTATCAGTAAACAA+TGG 0.660073 2_3:-54360399 None:intergenic
CCATTGTTTACTGATAACCA+TGG 0.663411 2_3:+54360399 Msa0299420:CDS
ATTCTGAGAAGCCAGAATGG+TGG 0.680045 2_3:+54360520 Msa0299420:CDS
ATTACGGGAGAGAAGGAAGG+CGG 0.686885 2_3:-54359148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATAAAAATAAAAAT+GGG - chr2_3:54360339-54360358 None:intergenic 0.0%
!! AATAAAAATAAAAATAAAAA+TGG - chr2_3:54360340-54360359 None:intergenic 0.0%
!! AGAATAATAATAATAAAAAG+AGG + chr2_3:54359014-54359033 Msa0299420:five_prime_UTR 10.0%
!! TTCAAATGAAAATATAAAAA+AGG - chr2_3:54359293-54359312 None:intergenic 10.0%
!!! TTCATTTTAATTTGAAATTA+AGG + chr2_3:54359422-54359441 Msa0299420:intron 10.0%
!! TTCTATTATATAAAGTTATA+AGG + chr2_3:54361113-54361132 Msa0299420:three_prime_UTR 10.0%
!! TCTATTATATAAAGTTATAA+GGG + chr2_3:54361114-54361133 Msa0299420:three_prime_UTR 10.0%
!!! TCTTTTCTTTAGTTTTTTTT+AGG - chr2_3:54358968-54358987 None:intergenic 15.0%
!! ATAATAATAATAAAAAGAGG+TGG + chr2_3:54359017-54359036 Msa0299420:five_prime_UTR 15.0%
!! TAATAATAATAAAAAGAGGT+GGG + chr2_3:54359018-54359037 Msa0299420:five_prime_UTR 15.0%
!! AAATGAAAATATAAAAAAGG+AGG - chr2_3:54359290-54359309 None:intergenic 15.0%
!!! AATGTATTTGATTTTTGATT+AGG + chr2_3:54359917-54359936 Msa0299420:intron 15.0%
!! TGCAATAATAATAATGAAAT+AGG + chr2_3:54360136-54360155 Msa0299420:intron 15.0%
!! AATAATAATAATGAAATAGG+AGG + chr2_3:54360139-54360158 Msa0299420:intron 15.0%
!!! TAAAAACTAGAAATAAGTTT+TGG - chr2_3:54360311-54360330 None:intergenic 15.0%
!!! TTTCTTTAGTTTTTTTTAGG+AGG - chr2_3:54358965-54358984 None:intergenic 20.0%
!! ATTGAATTGAATTGATGAAT+CGG - chr2_3:54359267-54359286 None:intergenic 20.0%
!!! ATGTTGTTATTTTTCAATTC+TGG + chr2_3:54359348-54359367 Msa0299420:intron 20.0%
!! AAAAATTAAAAGACAAACAG+AGG - chr2_3:54359882-54359901 None:intergenic 20.0%
!!! AAAACAATTTTGGATTTAGA+TGG - chr2_3:54360258-54360277 None:intergenic 20.0%
!!! ATGCAATTTGAAAACAATTT+TGG - chr2_3:54360268-54360287 None:intergenic 20.0%
!! AATTTGAATTTATCAAGCAA+AGG - chr2_3:54360615-54360634 None:intergenic 20.0%
!! TTTGGTATGACAAAATTTAT+AGG - chr2_3:54361256-54361275 None:intergenic 20.0%
! AGAGAAAGAAAAGGTAATTA+CGG - chr2_3:54359167-54359186 None:intergenic 25.0%
! AGTTGTAAAAGAGAAAGAAA+AGG - chr2_3:54359176-54359195 None:intergenic 25.0%
!! TGGTTATTTCATAGACTAAA+GGG - chr2_3:54359501-54359520 None:intergenic 25.0%
! TTTCAGTAAGAACATAAACA+AGG - chr2_3:54359712-54359731 None:intergenic 25.0%
!! TATTGTTGTTGTTGTTGTTA+TGG + chr2_3:54359825-54359844 Msa0299420:intron 25.0%
!!! TATCTGTCAATTTTAATCGT+AGG + chr2_3:54360069-54360088 Msa0299420:intron 25.0%
! GAAACTTGTATATTGATCTT+AGG + chr2_3:54360168-54360187 Msa0299420:intron 25.0%
!! AATTGTTTTCAAATTGCATG+TGG + chr2_3:54360269-54360288 Msa0299420:intron 25.0%
! CCAAATAGCAGTATAATATA+GGG - chr2_3:54360695-54360714 None:intergenic 25.0%
! GCATATTTGTAAACTATATG+TGG + chr2_3:54361033-54361052 Msa0299420:three_prime_UTR 25.0%
!! TCATACCAAAAATTCTAGAA+TGG + chr2_3:54361266-54361285 Msa0299420:three_prime_UTR 25.0%
ATAATAAAAAGAGGTGGGTA+AGG + chr2_3:54359023-54359042 Msa0299420:five_prime_UTR 30.0%
TAATAAAAAGAGGTGGGTAA+GGG + chr2_3:54359024-54359043 Msa0299420:five_prime_UTR 30.0%
GAGAAAGAAAAGGTAATTAC+GGG - chr2_3:54359166-54359185 None:intergenic 30.0%
! CTGGTTATTTCATAGACTAA+AGG - chr2_3:54359502-54359521 None:intergenic 30.0%
!!! GGTTTTAGAGTTTACTCTTT+AGG + chr2_3:54359656-54359675 Msa0299420:intron 30.0%
ACATTGCATAAATGTCTTCA+TGG - chr2_3:54360234-54360253 None:intergenic 30.0%
! TTCAAATTGCATGTGGTTTT+TGG + chr2_3:54360276-54360295 Msa0299420:intron 30.0%
CAATGGTATATATATGCTAG+TGG - chr2_3:54360477-54360496 None:intergenic 30.0%
CACTAGCATATATATACCAT+TGG + chr2_3:54360475-54360494 Msa0299420:CDS 30.0%
ATCTGCAATGCTATTAGATA+GGG - chr2_3:54360567-54360586 None:intergenic 30.0%
CCCTATATTATACTGCTATT+TGG + chr2_3:54360692-54360711 Msa0299420:CDS 30.0%
GCCAAATAGCAGTATAATAT+AGG - chr2_3:54360696-54360715 None:intergenic 30.0%
ACATGGAATCTGAAGATAAT+GGG - chr2_3:54360750-54360769 None:intergenic 30.0%
TACATGGAATCTGAAGATAA+TGG - chr2_3:54360751-54360770 None:intergenic 30.0%
TTCAGATTCCATGTAATGAT+GGG + chr2_3:54360756-54360775 Msa0299420:CDS 30.0%
AACACTTGGGATGTATTATT+GGG + chr2_3:54360851-54360870 Msa0299420:CDS 30.0%
CTATATTAAGTAGGGAATGA+AGG - chr2_3:54360977-54360996 None:intergenic 30.0%
CTTCATTCCCTACTTAATAT+AGG + chr2_3:54360975-54360994 Msa0299420:CDS 30.0%
ATTCCCTACTTAATATAGGA+GGG + chr2_3:54360979-54360998 Msa0299420:CDS 30.0%
GGGATATTGAAATTCTGATA+TGG + chr2_3:54361134-54361153 Msa0299420:three_prime_UTR 30.0%
! ATGCTCAAGCTTCTAAATTT+TGG - chr2_3:54361205-54361224 None:intergenic 30.0%
!!! CTAAGCCATTCTAGAATTTT+TGG - chr2_3:54361274-54361293 None:intergenic 30.0%
AAGAAGAAAGAAGACGATGA+TGG + chr2_3:54359073-54359092 Msa0299420:exon 35.0%
ATATGTTGTCTCTGTTCGTT+AGG + chr2_3:54359448-54359467 Msa0299420:intron 35.0%
! TCTCTGTTCGTTAGGTTTTA+TGG + chr2_3:54359456-54359475 Msa0299420:intron 35.0%
!! CTCTGTTCGTTAGGTTTTAT+GGG + chr2_3:54359457-54359476 Msa0299420:intron 35.0%
! GTTTTATGGGAGGGTTTAAT+AGG + chr2_3:54359470-54359489 Msa0299420:intron 35.0%
ATCAGAATTGTATCTGCTAC+AGG + chr2_3:54359634-54359653 Msa0299420:intron 35.0%
TCAGAATTGTATCTGCTACA+GGG + chr2_3:54359635-54359654 Msa0299420:intron 35.0%
TCTTTAGGTACTTTACCAGT+TGG + chr2_3:54359671-54359690 Msa0299420:intron 35.0%
AGATGTTTGTCTCATGATTC+AGG + chr2_3:54359785-54359804 Msa0299420:intron 35.0%
! GAGTTGTGTATGTTTTTCCT+TGG + chr2_3:54359852-54359871 Msa0299420:intron 35.0%
CATGGCTTAGCATCATATAA+AGG - chr2_3:54360216-54360235 None:intergenic 35.0%
CCATGGTTATCAGTAAACAA+TGG - chr2_3:54360402-54360421 None:intergenic 35.0%
CCATTGTTTACTGATAACCA+TGG + chr2_3:54360399-54360418 Msa0299420:CDS 35.0%
GCATATATATACCATTGGTC+TGG + chr2_3:54360480-54360499 Msa0299420:CDS 35.0%
CATCTGCAATGCTATTAGAT+AGG - chr2_3:54360568-54360587 None:intergenic 35.0%
! AATGTGATCTTTTACATCCC+AGG + chr2_3:54360642-54360661 Msa0299420:CDS 35.0%
TATAATATAGGGAACCAGGT+TGG - chr2_3:54360684-54360703 None:intergenic 35.0%
! ATACTGCTATTTGGCTATCA+TGG + chr2_3:54360701-54360720 Msa0299420:CDS 35.0%
!! TCATGGTAGTGGTGAAAAAT+AGG + chr2_3:54360718-54360737 Msa0299420:CDS 35.0%
!! CATGGTAGTGGTGAAAAATA+GGG + chr2_3:54360719-54360738 Msa0299420:CDS 35.0%
CATGGAATCTGAAGATAATG+GGG - chr2_3:54360749-54360768 None:intergenic 35.0%
CTTCAGATTCCATGTAATGA+TGG + chr2_3:54360755-54360774 Msa0299420:CDS 35.0%
AAATTGCTCTGCAGATTGTA+TGG + chr2_3:54360790-54360809 Msa0299420:CDS 35.0%
AATTGCTCTGCAGATTGTAT+GGG + chr2_3:54360791-54360810 Msa0299420:CDS 35.0%
GAACACTTGGGATGTATTAT+TGG + chr2_3:54360850-54360869 Msa0299420:CDS 35.0%
!! AAGCTGATTCGCTAATAACA+GGG - chr2_3:54360953-54360972 None:intergenic 35.0%
!! AAAGCTGATTCGCTAATAAC+AGG - chr2_3:54360954-54360973 None:intergenic 35.0%
CATTCCCTACTTAATATAGG+AGG + chr2_3:54360978-54360997 Msa0299420:CDS 35.0%
GAACCCTCCTATATTAAGTA+GGG - chr2_3:54360985-54361004 None:intergenic 35.0%
TTAATATAGGAGGGTTCCAA+CGG + chr2_3:54360988-54361007 Msa0299420:CDS 35.0%
CTCTTGACTACTTCCTAAAA+TGG - chr2_3:54361014-54361033 None:intergenic 35.0%
!! ATATGTGGTTTTGCAGTGAA+AGG + chr2_3:54361048-54361067 Msa0299420:three_prime_UTR 35.0%
!! TTCTAAATTTTGGCATGGCA+GGG - chr2_3:54361195-54361214 None:intergenic 35.0%
!! CAAGCTTCTAAATTTTGGCA+TGG - chr2_3:54361200-54361219 None:intergenic 35.0%
CTAGATCCTTCCACAGAAAA+AGG + chr2_3:54358911-54358930 Msa0299420:five_prime_UTR 40.0%
AAAGGTAATTACGGGAGAGA+AGG - chr2_3:54359158-54359177 None:intergenic 40.0%
!! TGTTCGTTAGGTTTTATGGG+AGG + chr2_3:54359460-54359479 Msa0299420:intron 40.0%
!! GTTCGTTAGGTTTTATGGGA+GGG + chr2_3:54359461-54359480 Msa0299420:intron 40.0%
CACGTATTCTCAAATCCAAC+TGG - chr2_3:54359689-54359708 None:intergenic 40.0%
AGACAAACAGAGGATAACCA+AGG - chr2_3:54359872-54359891 None:intergenic 40.0%
AGTACTAGAACTGCTTCTTC+GGG - chr2_3:54360451-54360470 None:intergenic 40.0%
GAGTACTAGAACTGCTTCTT+CGG - chr2_3:54360452-54360471 None:intergenic 40.0%
! CAAATTCTGAGAAGCCAGAA+TGG + chr2_3:54360517-54360536 Msa0299420:CDS 40.0%
GCAGTATAATATAGGGAACC+AGG - chr2_3:54360688-54360707 None:intergenic 40.0%
CTATTTGGCTATCATGGTAG+TGG + chr2_3:54360707-54360726 Msa0299420:CDS 40.0%
GTAGCATTCCCATCATTACA+TGG - chr2_3:54360767-54360786 None:intergenic 40.0%
! TGTAATGATGGGAATGCTAC+TGG + chr2_3:54360767-54360786 Msa0299420:CDS 40.0%
ATGCCACTGATACACTTCAA+GGG + chr2_3:54360828-54360847 Msa0299420:CDS 40.0%
ATACACTTCAAGGGAACACT+TGG + chr2_3:54360837-54360856 Msa0299420:CDS 40.0%
TACACTTCAAGGGAACACTT+GGG + chr2_3:54360838-54360857 Msa0299420:CDS 40.0%
TCTATCAGATGTGCTCTTCT+TGG + chr2_3:54360912-54360931 Msa0299420:CDS 40.0%
GGAACCCTCCTATATTAAGT+AGG - chr2_3:54360986-54361005 None:intergenic 40.0%
!! CTTCTAAATTTTGGCATGGC+AGG - chr2_3:54361196-54361215 None:intergenic 40.0%
!!! AGAAGCGTTGCCTTTTTCTG+TGG - chr2_3:54358924-54358943 None:intergenic 45.0%
AGGAGGACTTGTATGTCAGA+AGG - chr2_3:54358948-54358967 None:intergenic 45.0%
AAAGAGGTGGGTAAGGGTAA+CGG + chr2_3:54359030-54359049 Msa0299420:five_prime_UTR 45.0%
TAACGGAAGAATCGAGAGTC+AGG + chr2_3:54359047-54359066 Msa0299420:five_prime_UTR 45.0%
! AGAAAGAAGACGATGATGGC+CGG + chr2_3:54359077-54359096 Msa0299420:exon 45.0%
GTAATTACGGGAGAGAAGGA+AGG - chr2_3:54359154-54359173 None:intergenic 45.0%
AACGATGTCAGTGTCATGTC+TGG - chr2_3:54359521-54359540 None:intergenic 45.0%
!!! CATGTGGTTTTTGGCTACAG+TGG + chr2_3:54360285-54360304 Msa0299420:intron 45.0%
GGATTCGAAAACCAGACCAA+TGG - chr2_3:54360494-54360513 None:intergenic 45.0%
CTGGCTTCTCAGAATTTGCA+GGG - chr2_3:54360515-54360534 None:intergenic 45.0%
! TCTGGCTTCTCAGAATTTGC+AGG - chr2_3:54360516-54360535 None:intergenic 45.0%
! ATTCTGAGAAGCCAGAATGG+TGG + chr2_3:54360520-54360539 Msa0299420:CDS 45.0%
AAAGTTCTCCACCACCATTC+TGG - chr2_3:54360534-54360553 None:intergenic 45.0%
CATGCCACTGATACACTTCA+AGG + chr2_3:54360827-54360846 Msa0299420:CDS 45.0%
GTTCCCTTGAAGTGTATCAG+TGG - chr2_3:54360834-54360853 None:intergenic 45.0%
CTTGGGATGTATTATTGGGC+TGG + chr2_3:54360855-54360874 Msa0299420:CDS 45.0%
!! GATGTATTATTGGGCTGGTG+CGG + chr2_3:54360860-54360879 Msa0299420:CDS 45.0%
CTACTTCCTAAAATGGCCGT+TGG - chr2_3:54361007-54361026 None:intergenic 45.0%
!! GTGGTTTTGCAGTGAAAGGA+AGG + chr2_3:54361052-54361071 Msa0299420:three_prime_UTR 45.0%
!! TAAAAATAAAAATAAAAATG+GGG - chr2_3:54360338-54360357 None:intergenic 5.0%
!!! TTTATTTTTATTTTTATTTC+AGG + chr2_3:54360342-54360361 Msa0299420:intron 5.0%
! CTTCAATGCCCTCTTTGAGG+CGG - chr2_3:54358889-54358908 None:intergenic 50.0%
GAGCTTCAATGCCCTCTTTG+AGG - chr2_3:54358892-54358911 None:intergenic 50.0%
! GCGTTGCCTTTTTCTGTGGA+AGG - chr2_3:54358920-54358939 None:intergenic 50.0%
ATTACGGGAGAGAAGGAAGG+CGG - chr2_3:54359151-54359170 None:intergenic 50.0%
ACTAGAACTGCTTCTTCGGG+AGG - chr2_3:54360448-54360467 None:intergenic 50.0%
CATTGCAGATGTCTGCTGCT+GGG + chr2_3:54360577-54360596 Msa0299420:CDS 50.0%
! TAGGGAACCAGGTTGGCAAT+CGG - chr2_3:54360677-54360696 None:intergenic 50.0%
!! AGGGTTCCAACGGCCATTTT+AGG + chr2_3:54360998-54361017 Msa0299420:CDS 50.0%
! AGAAGCGTAGGTGGTGGTTG+AGG - chr2_3:54359123-54359142 None:intergenic 55.0%
! GAGAAGAGAAGCGTAGGTGG+TGG - chr2_3:54359129-54359148 None:intergenic 55.0%
! AAGGCGGAGAAGAGAAGCGT+AGG - chr2_3:54359135-54359154 None:intergenic 55.0%
CGTGTCTGACACGTCAGGTT+GGG - chr2_3:54359602-54359621 None:intergenic 55.0%
TCGTGTCTGACACGTCAGGT+TGG - chr2_3:54359603-54359622 None:intergenic 55.0%
GTGTTCGTGTCTGACACGTC+AGG - chr2_3:54359607-54359626 None:intergenic 55.0%
! CTTGCCATCACAGCTCTAGC+AGG + chr2_3:54359994-54360013 Msa0299420:CDS 55.0%
ACTGCCTGCTAGAGCTGTGA+TGG - chr2_3:54360001-54360020 None:intergenic 55.0%
! AGCTCTAGCAGGCAGTTCTC+TGG + chr2_3:54360005-54360024 Msa0299420:CDS 55.0%
GCTCTAGCAGGCAGTTCTCT+GGG + chr2_3:54360006-54360025 Msa0299420:CDS 55.0%
CACTGAGTGAGAGGTGACCA+TGG - chr2_3:54360419-54360438 None:intergenic 55.0%
!! AGGTTGGAGCACTGAGTGAG+AGG - chr2_3:54360428-54360447 None:intergenic 55.0%
GAACTGCTTCTTCGGGAGGT+TGG - chr2_3:54360444-54360463 None:intergenic 55.0%
CTGAGAAGCCAGAATGGTGG+TGG + chr2_3:54360523-54360542 Msa0299420:CDS 55.0%
GCATTGCAGATGTCTGCTGC+TGG + chr2_3:54360576-54360595 Msa0299420:CDS 55.0%
GCAATCGGTTGACAGCTCCT+GGG - chr2_3:54360662-54360681 None:intergenic 55.0%
CTGTCAACCGATTGCCAACC+TGG + chr2_3:54360667-54360686 Msa0299420:CDS 55.0%
GGCGGCTGCGGTTAATTGAC+CGG - chr2_3:54359099-54359118 None:intergenic 60.0%
! AGCGTAGGTGGTGGTTGAGG+CGG - chr2_3:54359120-54359139 None:intergenic 60.0%
! GCGGAGAAGAGAAGCGTAGG+TGG - chr2_3:54359132-54359151 None:intergenic 60.0%
GGCAATCGGTTGACAGCTCC+TGG - chr2_3:54360663-54360682 None:intergenic 60.0%
!! TTATTGGGCTGGTGCGGCGT+TGG + chr2_3:54360866-54360885 Msa0299420:CDS 60.0%
! GTAGGTGGTGGTTGAGGCGG+CGG - chr2_3:54359117-54359136 None:intergenic 65.0%
GGTGGTTGAGGCGGCGGCTG+CGG - chr2_3:54359111-54359130 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 54358879 54361316 54358879 ID=Msa0299420;Name=Msa0299420
chr2_3 mRNA 54358879 54361316 54358879 ID=Msa0299420-mRNA-1;Parent=Msa0299420;Name=Msa0299420-mRNA-1;_AED=0.17;_eAED=0.17;_QI=210|1|1|1|1|1|3|297|273
chr2_3 exon 54358879 54359165 54358879 ID=Msa0299420-mRNA-1:exon:17331;Parent=Msa0299420-mRNA-1
chr2_3 exon 54359939 54360027 54359939 ID=Msa0299420-mRNA-1:exon:17332;Parent=Msa0299420-mRNA-1
chr2_3 exon 54360364 54361316 54360364 ID=Msa0299420-mRNA-1:exon:17333;Parent=Msa0299420-mRNA-1
chr2_3 five_prime_UTR 54358879 54359088 54358879 ID=Msa0299420-mRNA-1:five_prime_utr;Parent=Msa0299420-mRNA-1
chr2_3 CDS 54359089 54359165 54359089 ID=Msa0299420-mRNA-1:cds;Parent=Msa0299420-mRNA-1
chr2_3 CDS 54359939 54360027 54359939 ID=Msa0299420-mRNA-1:cds;Parent=Msa0299420-mRNA-1
chr2_3 CDS 54360364 54361019 54360364 ID=Msa0299420-mRNA-1:cds;Parent=Msa0299420-mRNA-1
chr2_3 three_prime_UTR 54361020 54361316 54361020 ID=Msa0299420-mRNA-1:three_prime_utr;Parent=Msa0299420-mRNA-1
Gene Sequence

>Msa0299420

ATGATGGCCGGTCAATTAACCGCAGCCGCCGCCTCAACCACCACCTACGCTTCTCTTCTCCGCCTTCCTTCTCTCCCGAATAAAGATTTGATTCAACAAAGACGCGTTTCATTCAACGTGAAGAAAAAGAATCTTGCCATCACAGCTCTAGCAGGCAGTTCTCTGGGAAAATCAGCTACACGACTCACAGCTGTTTCATCTCCATTGTTTACTGATAACCATGGTCACCTCTCACTCAGTGCTCCAACCTCCCGAAGAAGCAGTTCTAGTACTCGTCCACTAGCATATATATACCATTGGTCTGGTTTTCGAATCCCTGCAAATTCTGAGAAGCCAGAATGGTGGTGGAGAACTTTATCATGTATACCCTATCTAATAGCATTGCAGATGTCTGCTGCTGGGTATTATCTAGAACCTTTGCTTGATAAATTCAAATTATTTGAAAATGTGATCTTTTACATCCCAGGAGCTGTCAACCGATTGCCAACCTGGTTCCCTATATTATACTGCTATTTGGCTATCATGGTAGTGGTGAAAAATAGGGACTTCCCCATTATCTTCAGATTCCATGTAATGATGGGAATGCTACTGGAAATTGCTCTGCAGATTGTATGGGTTGCAAGCAATTTCATGCCACTGATACACTTCAAGGGAACACTTGGGATGTATTATTGGGCTGGTGCGGCGTTGGCATATATTGTCATAATCATGCATTCTATCAGATGTGCTCTTCTTGGTACATTTTCGAACATCCCTGTTATTAGCGAATCAGCTTTCCTTCATTCCCTACTTAATATAGGAGGGTTCCAACGGCCATTTTAG
Protein sequence

>Msa0299420

MMAGQLTAAAASTTTYASLLRLPSLPNKDLIQQRRVSFNVKKKNLAITALAGSSLGKSATRLTAVSSPLFTDNHGHLSLSAPTSRRSSSSTRPLAYIYHWSGFRIPANSEKPEWWWRTLSCIPYLIALQMSAAGYYLEPLLDKFKLFENVIFYIPGAVNRLPTWFPILYCYLAIMVVVKNRDFPIIFRFHVMMGMLLEIALQIVWVASNFMPLIHFKGTLGMYYWAGAALAYIVIIMHSIRCALLGTFSNIPVISESAFLHSLLNIGGFQRPF*