AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0299670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299670 MtrunA17_Chr2g0324171 90.260 154 15 0 21 174 20 173 4.98e-90 278
Msa0299670 MtrunA17_Chr2g0324471 71.795 195 39 3 1 194 22 201 1.72e-87 271
Msa0299670 MtrunA17_Chr2g0324481 65.789 152 49 2 25 175 22 171 1.05e-56 190
Msa0299670 MtrunA17_Chr2g0282271 54.023 174 72 4 1 171 1 169 1.19e-44 157
Msa0299670 MtrunA17_Chr7g0239211 43.023 172 92 4 4 174 3 169 1.01e-33 126
Msa0299670 MtrunA17_Chr7g0239321 41.279 172 95 4 4 174 3 169 7.38e-33 124
Msa0299670 MtrunA17_Chr7g0239331 42.442 172 93 4 4 174 3 169 1.65e-28 111
Msa0299670 MtrunA17_Chr7g0239221 40.698 172 98 3 4 174 3 171 2.19e-28 111
Msa0299670 MtrunA17_Chr7g0239181 38.953 172 99 4 4 174 3 169 6.63e-28 110
Msa0299670 MtrunA17_Chr3g0112111 45.000 140 74 2 36 174 40 177 2.11e-26 105
Msa0299670 MtrunA17_Chr1g0172951 40.116 172 97 4 4 174 3 169 5.45e-26 104
Msa0299670 MtrunA17_Chr7g0239371 44.444 135 71 3 35 168 34 165 6.12e-26 104
Msa0299670 MtrunA17_Chr7g0239191 40.909 176 95 4 5 174 28 200 7.42e-26 104
Msa0299670 MtrunA17_Chr7g0239261 39.011 182 102 5 5 182 4 180 2.46e-25 102
Msa0299670 MtrunA17_Chr7g0239361 41.279 172 96 4 5 174 15 183 2.76e-25 102
Msa0299670 MtrunA17_Chr7g0239381 37.853 177 99 7 1 171 1 172 8.85e-24 98.2
Msa0299670 MtrunA17_Chr7g0239311 40.588 170 94 4 6 174 6 169 9.04e-24 98.2
Msa0299670 MtrunA17_Chr7g0239281 35.294 170 98 5 17 182 5 166 1.39e-23 97.4
Msa0299670 MtrunA17_Chr7g0239171 40.116 172 97 4 4 174 3 169 1.78e-22 94.4
Msa0299670 MtrunA17_Chr4g0058561 37.086 151 92 3 26 174 31 180 4.23e-20 87.8
Msa0299670 MtrunA17_Chr5g0414561 37.356 174 98 7 1 168 1 169 5.64e-20 87.4
Msa0299670 MtrunA17_Chr5g0433941 36.232 138 78 3 41 177 45 173 7.03e-15 72.4
Msa0299670 MtrunA17_Chr5g0407721 37.857 140 76 4 43 173 61 198 7.58e-15 72.4
Msa0299670 MtrunA17_Chr7g0239241 30.168 179 81 6 6 181 1 138 8.74e-14 68.9
Msa0299670 MtrunA17_Chr3g0096161 33.566 143 91 3 35 176 48 187 1.50e-12 64.7
Msa0299670 MtrunA17_Chr8g0366941 30.065 153 105 2 31 182 39 190 3.15e-12 64.3
Msa0299670 MtrunA17_Chr3g0100011 29.787 188 109 7 5 178 31 209 1.81e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299670 AT4G04960.1 53.947 152 67 2 21 171 18 167 4.44e-51 175
Msa0299670 AT4G28350.1 54.777 157 64 4 22 174 20 173 2.22e-48 167
Msa0299670 AT4G04960.2 53.947 152 67 2 21 171 18 167 2.65e-47 163
Msa0299670 AT3G16530.1 37.143 175 98 3 11 174 3 176 2.66e-31 115
Msa0299670 AT2G37710.1 45.695 151 78 3 26 174 27 175 2.08e-30 117
Msa0299670 AT4G29050.2 43.511 131 72 1 44 174 57 185 1.44e-28 112
Msa0299670 AT3G45440.2 37.097 186 102 7 11 188 58 236 1.68e-28 112
Msa0299670 AT4G29050.1 43.511 131 72 1 44 174 43 171 1.68e-28 112
Msa0299670 AT3G45440.3 37.569 181 98 7 11 183 5 178 2.39e-28 111
Msa0299670 AT3G45440.4 37.569 181 98 7 11 183 5 178 2.39e-28 111
Msa0299670 AT3G45440.1 37.569 181 98 7 11 183 5 178 2.39e-28 111
Msa0299670 AT2G43700.1 36.232 138 86 1 37 174 27 162 5.97e-28 110
Msa0299670 AT4G02420.1 47.143 140 69 3 36 174 39 174 1.96e-27 108
Msa0299670 AT2G43700.3 37.037 135 83 1 40 174 6 138 4.46e-27 108
Msa0299670 AT2G43700.5 37.037 135 83 1 40 174 6 138 4.46e-27 108
Msa0299670 AT2G43700.2 37.037 135 83 1 40 174 6 138 4.46e-27 108
Msa0299670 AT2G43700.4 37.037 135 83 1 40 174 6 138 4.46e-27 108
Msa0299670 AT4G02410.1 45.255 137 72 2 36 171 40 174 1.87e-26 106
Msa0299670 AT5G60310.1 36.207 174 103 4 6 178 9 175 3.92e-26 105
Msa0299670 AT1G53080.1 39.011 182 91 7 6 174 11 185 5.19e-26 101
Msa0299670 AT3G59700.1 39.844 128 75 1 47 174 38 163 5.77e-26 105
Msa0299670 AT5G03350.1 34.343 198 111 5 11 197 3 192 6.68e-26 101
Msa0299670 AT3G53810.1 39.744 156 88 4 16 168 17 169 2.40e-25 103
Msa0299670 AT2G29220.1 38.857 175 96 6 4 174 7 174 4.67e-25 102
Msa0299670 AT3G59730.2 38.125 160 87 4 16 174 11 159 6.37e-25 101
Msa0299670 AT3G59730.1 38.125 160 87 4 16 174 11 159 6.72e-25 101
Msa0299670 AT3G55550.1 42.857 140 76 3 36 174 37 173 7.59e-25 101
Msa0299670 AT3G45430.2 37.580 157 91 4 24 179 26 176 1.54e-24 100
Msa0299670 AT3G45430.3 37.580 157 91 4 24 179 26 176 1.54e-24 100
Msa0299670 AT3G45430.1 37.580 157 91 4 24 179 26 176 1.54e-24 100
Msa0299670 AT2G29250.1 40.881 159 88 4 20 175 20 175 4.62e-24 99.4
Msa0299670 AT3G59740.1 40.816 147 77 4 28 174 21 157 1.46e-23 98.2
Msa0299670 AT3G15356.1 37.748 151 85 2 32 174 27 176 4.75e-23 93.6
Msa0299670 AT5G60300.3 34.810 158 97 3 24 180 25 177 4.18e-22 94.0
Msa0299670 AT2G43690.1 35.616 146 92 1 29 174 20 163 4.35e-22 94.0
Msa0299670 AT2G43690.3 35.616 146 92 1 29 174 20 163 4.35e-22 94.0
Msa0299670 AT5G60300.1 34.810 158 97 3 24 180 25 177 5.03e-22 93.6
Msa0299670 AT5G60300.2 34.810 158 97 3 24 180 25 177 5.03e-22 93.6
Msa0299670 AT5G60280.1 36.095 169 101 4 6 174 10 171 5.90e-22 93.6
Msa0299670 AT5G60320.1 32.768 177 110 5 6 178 9 180 2.13e-21 91.7
Msa0299670 AT1G53070.1 46.324 136 63 3 37 164 38 171 4.03e-21 88.6
Msa0299670 AT1G70110.1 36.090 133 84 1 36 168 33 164 1.51e-20 89.4
Msa0299670 AT1G53060.1 38.514 148 80 4 37 174 1 147 1.96e-20 86.3
Msa0299670 AT3G59750.1 35.260 173 93 7 8 177 5 161 2.97e-20 88.6
Msa0299670 AT2G43690.2 38.281 128 77 1 47 174 2 127 7.10e-20 87.4
Msa0299670 AT5G01550.1 37.143 175 101 5 1 168 19 191 9.58e-20 87.0
Msa0299670 AT3G45410.1 34.184 196 110 10 5 188 4 192 1.27e-19 86.7
Msa0299670 AT3G45330.1 34.839 155 96 4 26 178 30 181 1.27e-18 84.0
Msa0299670 AT5G60270.1 35.429 175 99 6 10 178 6 172 1.56e-18 83.6
Msa0299670 AT3G08870.2 39.437 142 78 4 33 168 65 204 2.33e-18 83.2
Msa0299670 AT3G08870.1 39.437 142 78 4 33 168 44 183 2.42e-18 82.8
Msa0299670 AT3G45390.1 32.738 168 100 6 20 178 20 183 2.77e-18 82.8
Msa0299670 AT1G15530.1 36.943 157 89 5 22 171 28 181 8.60e-17 78.6
Msa0299670 AT5G01560.1 38.776 147 80 5 33 173 38 180 1.09e-16 78.2
Msa0299670 AT3G45420.1 32.597 181 108 8 7 181 7 179 2.40e-16 77.0
Msa0299670 AT5G01540.1 33.775 151 96 3 22 168 29 179 2.64e-15 74.3
Msa0299670 AT1G07460.2 36.257 171 97 5 7 174 12 173 3.19e-15 73.2
Msa0299670 AT5G03140.1 32.061 131 84 2 41 170 51 177 4.55e-15 73.6
Msa0299670 AT5G59270.2 32.778 180 112 6 1 178 8 180 5.15e-15 73.2
Msa0299670 AT5G59270.1 36.538 156 90 6 25 178 29 177 8.89e-15 72.8
Msa0299670 AT5G59270.3 34.615 156 93 6 25 178 32 180 9.25e-15 72.4
Msa0299670 AT1G07460.1 51.351 74 36 0 101 174 36 109 1.80e-14 70.5
Msa0299670 AT5G59260.1 31.313 198 121 9 7 198 12 200 3.60e-13 67.8
Msa0299670 AT3G53380.1 32.370 173 106 5 4 172 1 166 6.51e-13 67.0
Msa0299670 AT5G55830.1 30.337 178 107 6 5 171 6 177 9.24e-12 63.9

Find 35 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACCTTCAAGTGGTCTAAA+TGG 0.277261 2_3:+54822549 Msa0299670:CDS
GGCCCATAGCCTGTCGAGGC+TGG 0.285038 2_3:+54822743 Msa0299670:CDS
TCGAGGCTGGGCGACGGCAC+CGG 0.338144 2_3:+54822756 Msa0299670:CDS
TCTCTTACATCCTTATCTTC+AGG 0.347098 2_3:+54822717 Msa0299670:CDS
CGCCCAGCCTCGACAGGCTA+TGG 0.370575 2_3:-54822746 None:intergenic
GGAAACACAGCAGATAATTA+TGG 0.375097 2_3:-54822268 None:intergenic
GGGAGAAGAGTGGAAGAGTT+TGG 0.380117 2_3:-54822450 None:intergenic
GTCATTAGTGAGAGTAAGAA+TGG 0.385296 2_3:-54822367 None:intergenic
CGAGGCTGGGCGACGGCACC+GGG 0.394608 2_3:+54822757 Msa0299670:CDS
AAAATGAAGGAAGTAGCAAA+TGG 0.405656 2_3:-54822471 None:intergenic
CTCTTACATCCTTATCTTCA+GGG 0.446361 2_3:+54822718 Msa0299670:CDS
TGCCGTCGCCCAGCCTCGAC+AGG 0.459842 2_3:-54822752 None:intergenic
ACAACATCAACTGAGTATAT+CGG 0.459897 2_3:+54822573 Msa0299670:CDS
TACATCCTTATCTTCAGGGC+CGG 0.490899 2_3:+54822722 Msa0299670:CDS
GCCCATAGCCTGTCGAGGCT+GGG 0.493102 2_3:+54822744 Msa0299670:CDS
AAATGAAGGAAGTAGCAAAT+GGG 0.498637 2_3:-54822470 None:intergenic
GTTCCATTTAGACCACTTGA+AGG 0.502767 2_3:-54822552 None:intergenic
TAATGGTTGCATTTCCATGT+AGG 0.509118 2_3:-54822338 None:intergenic
AGTAGCAAATGGGAGAAGAG+TGG 0.510021 2_3:-54822460 None:intergenic
AGAAGAGTGGAAGAGTTTGG+TGG 0.510956 2_3:-54822447 None:intergenic
ATGGGCCGGCCCTGAAGATA+AGG 0.515536 2_3:-54822727 None:intergenic
AGCCTCGACAGGCTATGGGC+CGG 0.518702 2_3:-54822741 None:intergenic
TTCATATTCACACCTTCAAG+TGG 0.542279 2_3:+54822540 Msa0299670:CDS
AGTAAGAATGGAGTTGCTAA+TGG 0.545061 2_3:-54822355 None:intergenic
CACGAGCTATTGAGAAGGAA+GGG 0.552373 2_3:-54822395 None:intergenic
GATACTAATGATAATCATGT+TGG 0.552500 2_3:+54822681 Msa0299670:CDS
GCCCAGCCTCGACAGGCTAT+GGG 0.571043 2_3:-54822745 None:intergenic
GCACGAGCTATTGAGAAGGA+AGG 0.578111 2_3:-54822396 None:intergenic
ATTATAGACAAATTCAGTAG+TGG 0.581794 2_3:-54822289 None:intergenic
ACGAGCTATTGAGAAGGAAG+GGG 0.589492 2_3:-54822394 None:intergenic
AGCCTGTCGAGGCTGGGCGA+CGG 0.595903 2_3:+54822750 Msa0299670:CDS
ACTTGAAGGTGTGAATATGA+AGG 0.598797 2_3:-54822538 None:intergenic
GGCCGGCCCATAGCCTGTCG+AGG 0.603118 2_3:+54822739 Msa0299670:CDS
GTGATAAGAAGCTTAAGTTG+AGG 0.620742 2_3:-54822228 None:intergenic
AAAAGCACGAGCTATTGAGA+AGG 0.639292 2_3:-54822400 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACAAAAGAAAAATATTTT+TGG - chr2_3:54822800-54822819 None:intergenic 10.0%
!! TTCAAAAATATTATCCTACA+TGG + chr2_3:54822324-54822343 Msa0299670:CDS 20.0%
!!! GTTTTGTAGGTATTTTTTTA+GGG - chr2_3:54822428-54822447 None:intergenic 20.0%
! ATTATAGACAAATTCAGTAG+TGG - chr2_3:54822292-54822311 None:intergenic 25.0%
!!! GGTTTTGTAGGTATTTTTTT+AGG - chr2_3:54822429-54822448 None:intergenic 25.0%
!! TATGATAAAGTTTTTGATGG+GGG - chr2_3:54822505-54822524 None:intergenic 25.0%
!! CTATGATAAAGTTTTTGATG+GGG - chr2_3:54822506-54822525 None:intergenic 25.0%
!! CCTATGATAAAGTTTTTGAT+GGG - chr2_3:54822507-54822526 None:intergenic 25.0%
!! TCCTATGATAAAGTTTTTGA+TGG - chr2_3:54822508-54822527 None:intergenic 25.0%
! AAAAACTTTATCATAGGACA+TGG + chr2_3:54822510-54822529 Msa0299670:CDS 25.0%
!! CTTTTAAATCTCACAAATCA+AGG + chr2_3:54822597-54822616 Msa0299670:CDS 25.0%
! GATACTAATGATAATCATGT+TGG + chr2_3:54822681-54822700 Msa0299670:CDS 25.0%
AAATGAAGGAAGTAGCAAAT+GGG - chr2_3:54822473-54822492 None:intergenic 30.0%
AAAATGAAGGAAGTAGCAAA+TGG - chr2_3:54822474-54822493 None:intergenic 30.0%
CCCATCAAAAACTTTATCAT+AGG + chr2_3:54822504-54822523 Msa0299670:CDS 30.0%
ACAACATCAACTGAGTATAT+CGG + chr2_3:54822573-54822592 Msa0299670:CDS 30.0%
! AATCCTAACAACCATGTTTT+TGG + chr2_3:54822621-54822640 Msa0299670:CDS 30.0%
!!! TATTTTTGGCCCCAAAAAAT+TGG - chr2_3:54822786-54822805 None:intergenic 30.0%
! GTGATAAGAAGCTTAAGTTG+AGG - chr2_3:54822231-54822250 None:intergenic 35.0%
GGAAACACAGCAGATAATTA+TGG - chr2_3:54822271-54822290 None:intergenic 35.0%
TAATGGTTGCATTTCCATGT+AGG - chr2_3:54822341-54822360 None:intergenic 35.0%
AGTAAGAATGGAGTTGCTAA+TGG - chr2_3:54822358-54822377 None:intergenic 35.0%
GTCATTAGTGAGAGTAAGAA+TGG - chr2_3:54822370-54822389 None:intergenic 35.0%
! ACTTGAAGGTGTGAATATGA+AGG - chr2_3:54822541-54822560 None:intergenic 35.0%
TTCATATTCACACCTTCAAG+TGG + chr2_3:54822540-54822559 Msa0299670:CDS 35.0%
ACTCCAAAAACATGGTTGTT+AGG - chr2_3:54822627-54822646 None:intergenic 35.0%
TCTCTTACATCCTTATCTTC+AGG + chr2_3:54822717-54822736 Msa0299670:CDS 35.0%
CTCTTACATCCTTATCTTCA+GGG + chr2_3:54822718-54822737 Msa0299670:CDS 35.0%
! AAAAGCACGAGCTATTGAGA+AGG - chr2_3:54822403-54822422 None:intergenic 40.0%
!!! GAAGAGTTTGGTGGTTTTGT+AGG - chr2_3:54822441-54822460 None:intergenic 40.0%
ACACCTTCAAGTGGTCTAAA+TGG + chr2_3:54822549-54822568 Msa0299670:CDS 40.0%
GTTCCATTTAGACCACTTGA+AGG - chr2_3:54822555-54822574 None:intergenic 40.0%
CGAATTCGACTCCAAAAACA+TGG - chr2_3:54822635-54822654 None:intergenic 40.0%
! TTTTGGCCCCAAAAAATTGG+AGG - chr2_3:54822783-54822802 None:intergenic 40.0%
ACGAGCTATTGAGAAGGAAG+GGG - chr2_3:54822397-54822416 None:intergenic 45.0%
CACGAGCTATTGAGAAGGAA+GGG - chr2_3:54822398-54822417 None:intergenic 45.0%
AGAAGAGTGGAAGAGTTTGG+TGG - chr2_3:54822450-54822469 None:intergenic 45.0%
AGTAGCAAATGGGAGAAGAG+TGG - chr2_3:54822463-54822482 None:intergenic 45.0%
GGGGGAGATAGAGAAAATGA+AGG - chr2_3:54822487-54822506 None:intergenic 45.0%
TACATCCTTATCTTCAGGGC+CGG + chr2_3:54822722-54822741 Msa0299670:CDS 45.0%
GCACGAGCTATTGAGAAGGA+AGG - chr2_3:54822399-54822418 None:intergenic 50.0%
GGGAGAAGAGTGGAAGAGTT+TGG - chr2_3:54822453-54822472 None:intergenic 50.0%
!!! CACCGGGCCTCCAATTTTTT+GGG + chr2_3:54822773-54822792 Msa0299670:CDS 50.0%
!!! ACCGGGCCTCCAATTTTTTG+GGG + chr2_3:54822774-54822793 Msa0299670:CDS 50.0%
!!! GCACCGGGCCTCCAATTTTT+TGG + chr2_3:54822772-54822791 Msa0299670:CDS 55.0%
GCCCCAAAAAATTGGAGGCC+CGG - chr2_3:54822778-54822797 None:intergenic 55.0%
ATGGGCCGGCCCTGAAGATA+AGG - chr2_3:54822730-54822749 None:intergenic 60.0%
AGCCTCGACAGGCTATGGGC+CGG - chr2_3:54822744-54822763 None:intergenic 65.0%
GCCCATAGCCTGTCGAGGCT+GGG + chr2_3:54822744-54822763 Msa0299670:CDS 65.0%
GCCCAGCCTCGACAGGCTAT+GGG - chr2_3:54822748-54822767 None:intergenic 65.0%
GGCCCATAGCCTGTCGAGGC+TGG + chr2_3:54822743-54822762 Msa0299670:CDS 70.0%
CGCCCAGCCTCGACAGGCTA+TGG - chr2_3:54822749-54822768 None:intergenic 70.0%
AGCCTGTCGAGGCTGGGCGA+CGG + chr2_3:54822750-54822769 Msa0299670:CDS 70.0%
GGCCGGCCCATAGCCTGTCG+AGG + chr2_3:54822739-54822758 Msa0299670:CDS 75.0%
TGCCGTCGCCCAGCCTCGAC+AGG - chr2_3:54822755-54822774 None:intergenic 75.0%
TCGAGGCTGGGCGACGGCAC+CGG + chr2_3:54822756-54822775 Msa0299670:CDS 75.0%
! CGAGGCTGGGCGACGGCACC+GGG + chr2_3:54822757-54822776 Msa0299670:CDS 80.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 54822225 54822836 54822225 ID=Msa0299670;Name=Msa0299670
chr2_3 mRNA 54822225 54822836 54822225 ID=Msa0299670-mRNA-1;Parent=Msa0299670;Name=Msa0299670-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|-1|0|1|-1|1|1|0|203
chr2_3 exon 54822225 54822836 54822225 ID=Msa0299670-mRNA-1:exon:17507;Parent=Msa0299670-mRNA-1
chr2_3 CDS 54822225 54822836 54822225 ID=Msa0299670-mRNA-1:cds;Parent=Msa0299670-mRNA-1
Gene Sequence

>Msa0299670

ATGCCTCAACTTAAGCTTCTTATCACTTTTCTTCACACTGTAACCATAATTATCTGCTGTGTTTCCACTACTGAATTTGTCTATAATACAAACTTCAACTTCAAAAATATTATCCTACATGGAAATGCAACCATTAGCAACTCCATTCTTACTCTCACTAATGACACTTCCCCTTCCTTCTCAATAGCTCGTGCTTTTTACCCTAAAAAAATACCTACAAAACCACCAAACTCTTCCACTCTTCTCCCATTTGCTACTTCCTTCATTTTCTCTATCTCCCCCATCAAAAACTTTATCATAGGACATGGCTTTGCCTTCATATTCACACCTTCAAGTGGTCTAAATGGAACAACATCAACTGAGTATATCGGTCTTTTAAATCTCACAAATCAAGGTAATCCTAACAACCATGTTTTTGGAGTCGAATTCGATATAGTTAAAAACGAAGAATTCAATGATACTAATGATAATCATGTTGGTATCGACGTAAACTCTCTTACATCCTTATCTTCAGGGCCGGCCCATAGCCTGTCGAGGCTGGGCGACGGCACCGGGCCTCCAATTTTTTGGGGCCAAAAATATTTTTCTTTTGTATGTAAGACACGAAAATAA

Protein sequence

>Msa0299670

MPQLKLLITFLHTVTIIICCVSTTEFVYNTNFNFKNIILHGNATISNSILTLTNDTSPSFSIARAFYPKKIPTKPPNSSTLLPFATSFIFSISPIKNFIIGHGFAFIFTPSSGLNGTTSTEYIGLLNLTNQGNPNNHVFGVEFDIVKNEEFNDTNDNHVGIDVNSLTSLSSGPAHSLSRLGDGTGPPIFWGQKYFSFVCKTRK*