Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220490 | XP_013465075.1 | 92.836 | 670 | 48 | 0 | 21 | 690 | 20 | 689 | 0.0 | 1240 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220490 | sp|O49445|LRK72_ARATH | 52.550 | 647 | 287 | 10 | 25 | 661 | 25 | 661 | 0.0 | 665 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220490 | A0A072VLS1 | 92.836 | 670 | 48 | 0 | 21 | 690 | 20 | 689 | 0.0 | 1240 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042550 | Msa0220490 | 0.812770 | 3.587050e-51 | -8.615850e-47 |
| Msa0178510 | Msa0220490 | 0.831540 | 1.575749e-55 | -8.615850e-47 |
| Msa0187830 | Msa0220490 | 0.811416 | 7.079511e-51 | -8.615850e-47 |
| Msa0215590 | Msa0220490 | 0.831465 | 1.643992e-55 | -8.615850e-47 |
| Msa0220450 | Msa0220490 | 0.817843 | 2.671966e-52 | -8.615850e-47 |
| Msa0220490 | Msa0236070 | 0.803038 | 4.218721e-49 | -8.615850e-47 |
| Msa0220490 | Msa0244140 | 0.809606 | 1.741456e-50 | -8.615850e-47 |
| Msa0220490 | Msa0244190 | 0.802085 | 6.631563e-49 | -8.615850e-47 |
| Msa0220490 | Msa0255850 | 0.811687 | 6.179885e-51 | -8.615850e-47 |
| Msa0220490 | Msa0261820 | 0.807718 | 4.408256e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220490 | MtrunA17_Chr2g0324171 | 92.836 | 670 | 48 | 0 | 21 | 690 | 20 | 689 | 0.0 | 1240 |
| Msa0220490 | MtrunA17_Chr2g0324471 | 80.548 | 694 | 115 | 6 | 1 | 690 | 22 | 699 | 0.0 | 1114 |
| Msa0220490 | MtrunA17_Chr2g0324481 | 57.544 | 676 | 272 | 11 | 25 | 690 | 22 | 692 | 0.0 | 729 |
| Msa0220490 | MtrunA17_Chr2g0282271 | 55.147 | 680 | 282 | 11 | 6 | 670 | 7 | 678 | 0.0 | 699 |
| Msa0220490 | MtrunA17_Chr7g0239371 | 41.616 | 656 | 346 | 20 | 35 | 678 | 34 | 664 | 9.27e-155 | 463 |
| Msa0220490 | MtrunA17_Chr7g0239321 | 42.197 | 628 | 335 | 17 | 6 | 619 | 1 | 614 | 1.67e-153 | 460 |
| Msa0220490 | MtrunA17_Chr7g0239381 | 42.072 | 637 | 333 | 21 | 1 | 620 | 1 | 618 | 2.48e-153 | 459 |
| Msa0220490 | MtrunA17_Chr7g0239331 | 43.084 | 629 | 329 | 17 | 4 | 619 | 3 | 615 | 5.24e-151 | 453 |
| Msa0220490 | MtrunA17_Chr7g0239211 | 41.498 | 641 | 324 | 19 | 4 | 619 | 3 | 617 | 6.61e-151 | 453 |
| Msa0220490 | MtrunA17_Chr3g0112111 | 42.003 | 669 | 345 | 21 | 25 | 674 | 28 | 672 | 5.59e-146 | 441 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0220490 | AT4G28350.1 | 52.550 | 647 | 287 | 10 | 25 | 661 | 25 | 661 | 0.0 | 665 |
| Msa0220490 | AT4G04960.1 | 52.489 | 663 | 281 | 12 | 21 | 662 | 18 | 667 | 0.0 | 643 |
| Msa0220490 | AT4G04960.2 | 55.456 | 559 | 221 | 9 | 21 | 562 | 18 | 565 | 0.0 | 583 |
| Msa0220490 | AT3G53810.1 | 41.842 | 619 | 333 | 17 | 16 | 619 | 17 | 623 | 7.40e-154 | 461 |
| Msa0220490 | AT4G02420.1 | 43.261 | 601 | 308 | 16 | 36 | 618 | 39 | 624 | 2.81e-151 | 454 |
| Msa0220490 | AT4G02410.1 | 42.479 | 605 | 311 | 16 | 36 | 618 | 40 | 629 | 1.75e-149 | 450 |
| Msa0220490 | AT2G37710.1 | 42.810 | 612 | 324 | 15 | 26 | 623 | 27 | 626 | 4.91e-148 | 446 |
| Msa0220490 | AT3G55550.1 | 42.314 | 605 | 316 | 17 | 36 | 623 | 37 | 625 | 1.99e-147 | 445 |
| Msa0220490 | AT4G29050.1 | 40.440 | 591 | 320 | 16 | 44 | 617 | 43 | 618 | 9.74e-144 | 435 |
| Msa0220490 | AT4G29050.2 | 40.440 | 591 | 320 | 16 | 44 | 617 | 57 | 632 | 1.15e-143 | 435 |
Find 144 sgRNAs with CRISPR-Local
Find 352 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCACGAGCTATTGAGA+AGG | 0.639292 | 2_1:-68913814 | None:intergenic |
| AAAATGAAGGAAGTAGCAAA+TGG | 0.405656 | 2_1:-68913885 | None:intergenic |
| AAAGTAGGGTGATCTCTATA+TGG | 0.429200 | 2_1:-68915589 | None:intergenic |
| AAATGAAGGAAGTAGCAAAT+GGG | 0.498637 | 2_1:-68913884 | None:intergenic |
| AACGTTACAATGGCTCGGGC+AGG | 0.535528 | 2_1:+68914260 | Msa0220490:CDS |
| AACTAGAGTATTGGCCACAT+AGG | 0.602477 | 2_1:+68914618 | Msa0220490:CDS |
| AAGAAGGAAAGATGCTAACA+TGG | 0.553216 | 2_1:+68914942 | Msa0220490:CDS |
| AAGATTGGGAACTAGAGTAT+TGG | 0.513958 | 2_1:+68914609 | Msa0220490:CDS |
| AAGCAATGTTCTACTTGACA+AGG | 0.630884 | 2_1:+68915060 | Msa0220490:CDS |
| AAGGATATGAATGCTAGATT+GGG | 0.432577 | 2_1:+68915079 | Msa0220490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATGAAGGAAGTAGCAAAT+GGG | - | chr2_1:68913887-68913906 | None:intergenic | 30.0% | |
| AAAATGAAGGAAGTAGCAAA+TGG | - | chr2_1:68913888-68913907 | None:intergenic | 30.0% | |
| CCCATCAAAAACTTTATCAT+AGG | + | chr2_1:68913918-68913937 | Msa0220490:CDS | 30.0% | |
| ACAACATCAACTGAGTATAT+CGG | + | chr2_1:68913987-68914006 | Msa0220490:CDS | 30.0% | |
| AATGTGTTGAACATGAAAAG+TGG | + | chr2_1:68914194-68914213 | Msa0220490:CDS | 30.0% | |
| GTGGAGAAAATTATCAAGTA+TGG | + | chr2_1:68914213-68914232 | Msa0220490:CDS | 30.0% | |
| TTAAGATTCATGCTGATAAG+AGG | - | chr2_1:68914305-68914324 | None:intergenic | 30.0% | |
| ATAGTGTTAACATTCTTGCT+TGG | + | chr2_1:68914390-68914409 | Msa0220490:CDS | 30.0% | |
| GAGAAGAAGAAATTGAAGAT+TGG | + | chr2_1:68914594-68914613 | Msa0220490:CDS | 30.0% | |
| AAGGATATGAATGCTAGATT+GGG | + | chr2_1:68915079-68915098 | Msa0220490:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 68913639 | 68915711 | 68913639 | ID=Msa0220490;Name=Msa0220490 |
| chr2_1 | mRNA | 68913639 | 68915711 | 68913639 | ID=Msa0220490-mRNA-1;Parent=Msa0220490;Name=Msa0220490-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|690 |
| chr2_1 | exon | 68913639 | 68915711 | 68913639 | ID=Msa0220490-mRNA-1:exon:19486;Parent=Msa0220490-mRNA-1 |
| chr2_1 | CDS | 68913639 | 68915711 | 68913639 | ID=Msa0220490-mRNA-1:cds;Parent=Msa0220490-mRNA-1 |
| Gene Sequence |
>Msa0220490 ATGCCTCAACTTAAGCTTTTTATCACTTTTCTTCACACTGTAACCATAATTATCTGCTGTGTTTCCACTACTGAATTTGTCTATAATACAAACTTCAACTTCAAAAATATTATCCTACATGGAAATGCAACCATTAGCAACTCCATTCTTACTCTCACTAATGACACTTCCCCTTCCTTCTCAATAGCTCGTGCTTTTTACCCTAAAAAAATACCTACAAAACCACCAAACTCTTCCACTCTTCTCCCATTTGCTACTTCCTTCATTTTCTCTATCTCCCCCATCAAAAACTTTATCATAGGACATGGCTTTGCCTTCATATTCACACCTTCAAGTGGTCTAAATGGAACAACATCAACTGAGTATATCGGTCTTTTAAATCTCACAAATCAAGGTAATCCTAACAACCATGTTTTTGGAGTCGAATTCGATATAGTTAAAAACGAAGAATTCAATGATACTAATGATAATCATGTTGGTATCGACGTAAACTCTCTTACATCCTTATCTTCACATGAGGCTGGCTATTGGGGTGGAAAACATGACAAGAAATTTAATGTGTTGAACATGAAAAGTGGAGAAAATTATCAAGTATGGATTGAATTTATGCACTCTCAGATAAACGTTACAATGGCTCGGGCAGGACGAAAGAGGCCACATGTGCCTCTTATCAGCATGAATCTTAACCTTTCTGGAGTTATTATGGATGAAACTTATGTTGGATTCTGTGCAGCCACGGGGAAGAAAAGAGATAGTGTTAACATTCTTGCTTGGAGCTTTAGTAATACAAATTTTTCAATTGGTGATGCTTTAGTAACACACAACATGCCTTCATTTGTCCCTCATAAAGGATGGTTTTTCGGAGCAAAGGCTATAGATGTAGGAATAACTGGTGTTGTCTCTGTCTTAATCATTAGTTGTGCAGTGTTATTTATTCTCTATAAGAGAAACAAAGGAGAAGAAGAAATTGAAGATTGGGAACTAGAGTATTGGCCACATAGGATTAGTTTCCAAGAGATTCATGCTGCAACAGGAGGATTCTCTGAAGAAAATGTGGTTGCTGTAGGAGAGAACAAAAGTGTATATAAAGGGATTTTGCAAGGTGTTGAAGTCGCGGTCAAGAGAATCCCTCAAGAAAGAGAAGGGATGAGAGAGTTTTTGGCTGAGGTTTCAAGCCTAGGAAGACTGAAACATAGAAATTTAGTGGGATTCAGAGGTTGGTGCAAGGAAGAAAAGGGAAACTTGATTCTAGTTTATCACTTCATGAATAATGGAAGTTTAGACAAATGGATCTTTGAGTGTGAAGAAGGAAAGATGCTAACATGGGAAGAGAGGATTCAAGTTTTGAAAAATGTAGCCACGGGAATTCTATACTTGCATGAGGGTTGGGAAGTTAAGGTTTTGCATAGGGATATCAAAGCAAGCAATGTTCTACTTGACAAGGATATGAATGCTAGATTGGGGGATTTTGGATTGGCTCTCATGCATGAGCATCACGGACAAGTGGCCAACACAACAAAAGTACTCGGAACATTAGGATACATTGCTCCAGAAGTGATTCGAACAGGAAGATCATCAACTATGTCTGATGTGTTTGGGTTTGGAATATTGGTGTTGGAAGTGATTTGCGGCCGAAGACCTATTGAAGAACATAAGCCAGGACTAATTGAATGGGTGGAGTCCCTAAAGGCGCTTGATCAACTTCACAGTGCTATTGATGAAAGGTTGAAGGCTAAGGGAGGATACACTATTGAGGAAGCTGAGAGATTGCTTCATTTGGGTTTGCTGTGTTCAAATTCAGACCCTGGTGTTAGACCAACAATGAGACAGGTTGTGAAAATGTTAGAGGGAGAAATGGAAAGCATTGAATCTGATGGTGAAAATATGGAGAAAAGTTTACTTGGAAGAATAAAGTCAGCTGCAACGTGGTCTAGAACTGAAAGTACACTTCCATATAGAGATCACCCTACTTTTGAAGAAATTAGAATGTTCAGTTATAATTCTAAGACGTCTACGAGTGGCTCAAGTTCCATTCCGCCATCAGATTCAGATATCATCAAGGAAGGCCAGTAA |
| Protein sequence |
>Msa0220490 MPQLKLFITFLHTVTIIICCVSTTEFVYNTNFNFKNIILHGNATISNSILTLTNDTSPSFSIARAFYPKKIPTKPPNSSTLLPFATSFIFSISPIKNFIIGHGFAFIFTPSSGLNGTTSTEYIGLLNLTNQGNPNNHVFGVEFDIVKNEEFNDTNDNHVGIDVNSLTSLSSHEAGYWGGKHDKKFNVLNMKSGENYQVWIEFMHSQINVTMARAGRKRPHVPLISMNLNLSGVIMDETYVGFCAATGKKRDSVNILAWSFSNTNFSIGDALVTHNMPSFVPHKGWFFGAKAIDVGITGVVSVLIISCAVLFILYKRNKGEEEIEDWELEYWPHRISFQEIHAATGGFSEENVVAVGENKSVYKGILQGVEVAVKRIPQEREGMREFLAEVSSLGRLKHRNLVGFRGWCKEEKGNLILVYHFMNNGSLDKWIFECEEGKMLTWEERIQVLKNVATGILYLHEGWEVKVLHRDIKASNVLLDKDMNARLGDFGLALMHEHHGQVANTTKVLGTLGYIAPEVIRTGRSSTMSDVFGFGILVLEVICGRRPIEEHKPGLIEWVESLKALDQLHSAIDERLKAKGGYTIEEAERLLHLGLLCSNSDPGVRPTMRQVVKMLEGEMESIESDGENMEKSLLGRIKSAATWSRTESTLPYRDHPTFEEIRMFSYNSKTSTSGSSSIPPSDSDIIKEGQ* |
