AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300460 MtrunA17_Chr2g0325521 98.381 247 4 0 1 247 1 247 7.22e-180 493
Msa0300460 MtrunA17_Chr4g0041051 71.875 256 63 3 1 247 1 256 6.45e-121 344
Msa0300460 MtrunA17_Chr4g0045901 44.672 244 124 6 2 235 13 255 4.52e-51 167
Msa0300460 MtrunA17_Chr7g0255961 47.253 182 86 4 54 234 70 242 3.74e-42 144
Msa0300460 MtrunA17_Chr1g0198801 40.594 202 109 3 45 242 144 338 2.16e-34 126
Msa0300460 MtrunA17_Chr3g0113721 33.333 201 115 5 46 234 26 219 7.00e-31 114
Msa0300460 MtrunA17_Chr5g0412371 38.462 143 74 3 54 188 40 176 4.12e-26 101
Msa0300460 MtrunA17_Chr5g0427361 40.606 165 72 2 49 188 27 190 4.73e-25 99.4
Msa0300460 MtrunA17_Chr2g0283714 51.250 80 36 1 91 170 16 92 3.47e-20 83.6
Msa0300460 MtrunA17_Chr5g0411341 40.244 82 49 0 28 109 459 540 3.21e-12 65.9
Msa0300460 MtrunA17_Chr8g0366751 39.506 81 49 0 29 109 429 509 8.35e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300460 AT1G68810.1 41.327 196 107 5 45 236 160 351 2.27e-33 124
Msa0300460 AT3G25710.1 46.622 148 70 2 45 188 118 260 1.01e-31 119
Msa0300460 AT3G56770.1 38.857 175 98 4 51 223 37 204 1.60e-30 113
Msa0300460 AT2G40200.1 39.634 164 89 3 61 219 65 223 1.99e-29 111
Msa0300460 AT2G41130.1 40.571 175 96 3 51 223 59 227 4.30e-27 105
Msa0300460 AT4G38070.1 36.154 130 76 2 59 188 92 214 1.29e-17 80.1
Msa0300460 AT4G17880.1 40.789 76 45 0 39 114 393 468 2.24e-11 63.5
Msa0300460 AT5G46760.1 44.156 77 42 1 39 114 391 467 2.29e-11 63.5
Msa0300460 AT1G32640.1 36.634 101 61 2 33 130 421 521 3.64e-11 63.2

Find 67 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCTTCTTCACTGGTTT+TGG 0.180837 2_3:-55910041 Msa0300460:CDS
CACTTCATCTTATGATAATA+AGG 0.194458 2_3:-55908778 Msa0300460:CDS
ATTGAATGATGAAAAGAAAA+AGG 0.226203 2_3:+55910288 None:intergenic
AATGATGCTCTAATTGAATA+AGG 0.233216 2_3:+55908854 None:intergenic
CTTATCTCATCACTGTCTTT+TGG 0.234136 2_3:+55908911 None:intergenic
ATATTCAGTTCTTCATCTTT+AGG 0.245592 2_3:-55908563 Msa0300460:CDS
TGTTGTGGTTGGAGTGTATT+TGG 0.260273 2_3:+55910329 None:intergenic
CCAAGAGGGTGTTCAGAATA+TGG 0.263756 2_3:+55910156 None:intergenic
TTTGCTGTTGCTGCTTCTTC+TGG 0.265344 2_3:+55910430 None:intergenic
GACTGTTATATTTGTGTGTT+TGG 0.277090 2_3:+55910240 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.279597 2_3:-55908881 Msa0300460:CDS
TTCACATGTAATGTGAATCT+TGG 0.305383 2_3:-55908508 Msa0300460:three_prime_UTR
ACAATGAATGAACTCATTCT+AGG 0.319427 2_3:-55908404 Msa0300460:three_prime_UTR
TTTCAAGGTGTAAATGGAAA+TGG 0.359014 2_3:-55910070 Msa0300460:CDS
TGTGGAGGCAGCTGTGAAAT+TGG 0.360925 2_3:-55909999 Msa0300460:CDS
TGTTATCCCAGGTGCTAATA+AGG 0.365921 2_3:-55909867 Msa0300460:intron
TGTAATAACTTCAGCTAGTA+AGG 0.369694 2_3:+55908988 None:intergenic
AAGGGCAGAGATTGCAACTT+TGG 0.372459 2_3:-55908759 Msa0300460:CDS
GTAAAGATTACTTCTTATAG+TGG 0.377802 2_3:+55910452 None:intergenic
ACTCTCTCTGCCTCTGCCTC+TGG 0.380422 2_3:-55910401 Msa0300460:five_prime_UTR
TATGATGATGATAGTGAGTT+TGG 0.389810 2_3:+55910372 None:intergenic
GAGTATCGACAGTTTCTTGC+TGG 0.401116 2_3:-55908674 Msa0300460:CDS
CAAAGCTTCAATACTTCTCT+CGG 0.423960 2_3:+55909958 None:intergenic
AGGGCAGAGATTGCAACTTT+GGG 0.424505 2_3:-55908758 Msa0300460:CDS
TCTTATATCAGACAATAAAC+CGG 0.424693 2_3:+55908814 None:intergenic
CCATATTCTGAACACCCTCT+TGG 0.428144 2_3:-55910156 Msa0300460:five_prime_UTR
GGTGTAAATGGAAATGGATA+TGG 0.430429 2_3:-55910064 Msa0300460:CDS
TTAATTTGTCATGTTGTGGT+TGG 0.444797 2_3:+55910318 None:intergenic
ATGATGCTCTAATTGAATAA+GGG 0.448376 2_3:+55908855 None:intergenic
ATCTTGGGTTGTACATTTGT+TGG 0.451003 2_3:-55908492 Msa0300460:three_prime_UTR
ATATGGTTCATCTTCTTCAC+TGG 0.451383 2_3:-55910047 Msa0300460:CDS
TCTTGGGTTGTACATTTGTT+GGG 0.456987 2_3:-55908491 Msa0300460:three_prime_UTR
CATATTCTGAACACCCTCTT+GGG 0.457908 2_3:-55910155 Msa0300460:five_prime_UTR
TAAAGATTACTTCTTATAGT+GGG 0.485015 2_3:+55910453 None:intergenic
ACTTCATCTTATGATAATAA+GGG 0.489015 2_3:-55908777 Msa0300460:CDS
TCGACAGTTTCTTGCTGGAT+CGG 0.489881 2_3:-55908669 Msa0300460:CDS
CTTATATCAGACAATAAACC+GGG 0.504302 2_3:+55908815 None:intergenic
TCACATGTAATGTGAATCTT+GGG 0.510126 2_3:-55908507 Msa0300460:three_prime_UTR
GGAATAGAAGTTATGTCAGC+AGG 0.512851 2_3:+55908354 None:intergenic
GAACTAACCTTATTAGCACC+TGG 0.521161 2_3:+55909860 None:intergenic
TTGGTGAGATCCAGAGGCAG+AGG 0.526086 2_3:+55910391 None:intergenic
GTTGTTAATTTGTCATGTTG+TGG 0.533979 2_3:+55910314 None:intergenic
TTATATTTGTGTGTTTGGAA+TGG 0.537796 2_3:+55910245 None:intergenic
AAAGCTTCAATACTTCTCTC+GGG 0.540932 2_3:+55909959 None:intergenic
AGCAAGAAACTGTCGATACT+CGG 0.542573 2_3:+55908676 None:intergenic
TTGGAAGAAGAAGAAGGATG+AGG 0.553860 2_3:+55910210 None:intergenic
TTATGTTGTGAATACAAACC+CGG 0.569012 2_3:-55908833 Msa0300460:CDS
GTGAGTTTGGTGAGATCCAG+AGG 0.570542 2_3:+55910385 None:intergenic
AACTAACCTTATTAGCACCT+GGG 0.573545 2_3:+55909861 None:intergenic
ACAACGTTGGAAGAAGAAGA+AGG 0.578318 2_3:+55910204 None:intergenic
AATGCAGCACAAGCAAGTGA+AGG 0.583141 2_3:-55908944 Msa0300460:CDS
AAAGATTACTTCTTATAGTG+GGG 0.583347 2_3:+55910454 None:intergenic
GCTTCAAGTAGAATGAATGA+TGG 0.589941 2_3:-55910097 Msa0300460:CDS
GCAGAGATTGCAACTTTGGG+AGG 0.592208 2_3:-55908755 Msa0300460:CDS
ATGCAGCACAAGCAAGTGAA+GGG 0.603381 2_3:-55908943 Msa0300460:CDS
ATCATAGTGAAGCAGAGAGG+AGG 0.603979 2_3:-55909931 Msa0300460:CDS
TCTTAGAAACTAGAAAGAGA+AGG 0.613686 2_3:-55908595 Msa0300460:CDS
TAGAAACTAGAAAGAGAAGG+AGG 0.614835 2_3:-55908592 Msa0300460:CDS
AAGATTACTTCTTATAGTGG+GGG 0.618050 2_3:+55910455 None:intergenic
ATAAGAGTTGAATCACAAGA+AGG 0.621854 2_3:-55908893 Msa0300460:CDS
ACACTTCGTAGTGTTATCCC+AGG 0.622939 2_3:-55909878 Msa0300460:CDS
AGAGTTGAATCACAAGAAGG+TGG 0.624818 2_3:-55908890 Msa0300460:CDS
GACACAAAGAAAGACAACGT+TGG 0.650206 2_3:+55910191 None:intergenic
AAACAACACAGTTCCCAAGA+GGG 0.661371 2_3:+55910142 None:intergenic
AAAACAACACAGTTCCCAAG+AGG 0.688172 2_3:+55910141 None:intergenic
GCTGTGAAATTGGAACGCAA+AGG 0.704659 2_3:-55909989 Msa0300460:CDS
AGAATCATAGTGAAGCAGAG+AGG 0.708784 2_3:-55909934 Msa0300460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGAATAGAATAATTTAATTT+TGG - chr2_3:55909608-55909627 Msa0300460:intron 15.0%
!!! GGAATAGAATAATTTAATTT+TGG - chr2_3:55909608-55909627 Msa0300460:intron 15.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_3:55908356-55908375 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_3:55908520-55908539 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_3:55908521-55908540 None:intergenic 20.0%
!! ATTAACTCCAAATATTTGAA+GGG - chr2_3:55909066-55909085 Msa0300460:intron 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_3:55909587-55909606 Msa0300460:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_3:55910029-55910048 Msa0300460:CDS 20.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_3:55908356-55908375 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_3:55908520-55908539 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_3:55908521-55908540 None:intergenic 20.0%
!! ATTAACTCCAAATATTTGAA+GGG - chr2_3:55909066-55909085 Msa0300460:intron 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_3:55909587-55909606 Msa0300460:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_3:55910029-55910048 Msa0300460:CDS 20.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_3:55908357-55908376 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG + chr2_3:55908564-55908583 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_3:55909011-55909030 Msa0300460:intron 25.0%
! GATTAACTCCAAATATTTGA+AGG - chr2_3:55909065-55909084 Msa0300460:intron 25.0%
! ATTCTTTCCCTTCAAATATT+TGG + chr2_3:55909076-55909095 None:intergenic 25.0%
! ACACTTTAATAGGGAATATA+AGG - chr2_3:55909281-55909300 Msa0300460:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_3:55909311-55909330 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG - chr2_3:55909417-55909436 Msa0300460:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_3:55909555-55909574 Msa0300460:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_3:55909582-55909601 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_3:55909586-55909605 Msa0300460:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_3:55909600-55909619 None:intergenic 25.0%
! AAGTATAAGCATAACTATGT+TGG + chr2_3:55909691-55909710 None:intergenic 25.0%
! AACATAGTTATGCTTATACT+TGG - chr2_3:55909690-55909709 Msa0300460:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_3:55909954-55909973 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_3:55909955-55909974 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_3:55909995-55910014 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_3:55910028-55910047 Msa0300460:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_3:55910243-55910262 Msa0300460:five_prime_UTR 25.0%
! AAAGATTACTTCTTATAGTG+GGG + chr2_3:55908355-55908374 None:intergenic 25.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_3:55908357-55908376 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG + chr2_3:55908564-55908583 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_3:55909011-55909030 Msa0300460:intron 25.0%
! GATTAACTCCAAATATTTGA+AGG - chr2_3:55909065-55909084 Msa0300460:intron 25.0%
! ATTCTTTCCCTTCAAATATT+TGG + chr2_3:55909076-55909095 None:intergenic 25.0%
! ACACTTTAATAGGGAATATA+AGG - chr2_3:55909281-55909300 Msa0300460:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_3:55909311-55909330 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG - chr2_3:55909417-55909436 Msa0300460:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_3:55909555-55909574 Msa0300460:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_3:55909582-55909601 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_3:55909586-55909605 Msa0300460:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_3:55909600-55909619 None:intergenic 25.0%
! AAGTATAAGCATAACTATGT+TGG + chr2_3:55909691-55909710 None:intergenic 25.0%
! AACATAGTTATGCTTATACT+TGG - chr2_3:55909690-55909709 Msa0300460:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_3:55909954-55909973 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_3:55909955-55909974 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_3:55909995-55910014 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_3:55910028-55910047 Msa0300460:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_3:55910243-55910262 Msa0300460:five_prime_UTR 25.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_3:55908437-55908456 None:intergenic 30.0%
!! TTAATTTGTCATGTTGTGGT+TGG + chr2_3:55908491-55908510 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+TGG + chr2_3:55908495-55908514 None:intergenic 30.0%
GACTGTTATATTTGTGTGTT+TGG + chr2_3:55908569-55908588 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_3:55908678-55908697 Msa0300460:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_3:55908721-55908740 Msa0300460:CDS 30.0%
TTTCAAGGTGTAAATGGAAA+TGG - chr2_3:55908736-55908755 Msa0300460:CDS 30.0%
TGAAGACTCACACTTTAATA+GGG - chr2_3:55909272-55909291 Msa0300460:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_3:55909312-55909331 None:intergenic 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_3:55909456-55909475 Msa0300460:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_3:55909468-55909487 Msa0300460:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_3:55909496-55909515 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_3:55909514-55909533 None:intergenic 30.0%
!! GTGTACTTTTTGAACTTAGT+TGG - chr2_3:55909786-55909805 Msa0300460:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr2_3:55909821-55909840 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_3:55909913-55909932 Msa0300460:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_3:55909973-55909992 Msa0300460:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_3:55909994-55910013 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_3:55910211-55910230 Msa0300460:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_3:55910298-55910317 Msa0300460:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_3:55910299-55910318 Msa0300460:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_3:55910402-55910421 Msa0300460:five_prime_UTR 30.0%
AAGATTACTTCTTATAGTGG+GGG + chr2_3:55908354-55908373 None:intergenic 30.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_3:55908437-55908456 None:intergenic 30.0%
!! TTAATTTGTCATGTTGTGGT+TGG + chr2_3:55908491-55908510 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+TGG + chr2_3:55908495-55908514 None:intergenic 30.0%
GACTGTTATATTTGTGTGTT+TGG + chr2_3:55908569-55908588 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_3:55908678-55908697 Msa0300460:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_3:55908721-55908740 Msa0300460:CDS 30.0%
TTTCAAGGTGTAAATGGAAA+TGG - chr2_3:55908736-55908755 Msa0300460:CDS 30.0%
TGAAGACTCACACTTTAATA+GGG - chr2_3:55909272-55909291 Msa0300460:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_3:55909312-55909331 None:intergenic 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_3:55909456-55909475 Msa0300460:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_3:55909468-55909487 Msa0300460:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_3:55909496-55909515 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_3:55909514-55909533 None:intergenic 30.0%
!! GTGTACTTTTTGAACTTAGT+TGG - chr2_3:55909786-55909805 Msa0300460:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr2_3:55909821-55909840 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_3:55909913-55909932 Msa0300460:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_3:55909973-55909992 Msa0300460:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_3:55909994-55910013 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_3:55910211-55910230 Msa0300460:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_3:55910298-55910317 Msa0300460:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_3:55910299-55910318 Msa0300460:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_3:55910402-55910421 Msa0300460:five_prime_UTR 30.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_3:55908672-55908691 Msa0300460:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_3:55908709-55908728 Msa0300460:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_3:55908730-55908749 Msa0300460:CDS 35.0%
GGTGTAAATGGAAATGGATA+TGG - chr2_3:55908742-55908761 Msa0300460:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_3:55908759-55908778 Msa0300460:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_3:55908765-55908784 Msa0300460:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_3:55908778-55908797 Msa0300460:CDS 35.0%
AAAGCTTCAATACTTCTCTC+GGG + chr2_3:55908850-55908869 None:intergenic 35.0%
CAAAGCTTCAATACTTCTCT+CGG + chr2_3:55908851-55908870 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG + chr2_3:55908948-55908967 None:intergenic 35.0%
!!! GTCTAGTTTTTGTACTTCGT+AGG + chr2_3:55909171-55909190 None:intergenic 35.0%
ATTAAAGTGTGAGTCTTCAC+AGG + chr2_3:55909271-55909290 None:intergenic 35.0%
GTGAAGACTCACACTTTAAT+AGG - chr2_3:55909271-55909290 Msa0300460:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_3:55909467-55909486 Msa0300460:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_3:55909898-55909917 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_3:55909925-55909944 Msa0300460:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_3:55910214-55910233 Msa0300460:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_3:55910314-55910333 Msa0300460:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_3:55910315-55910334 Msa0300460:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_3:55910335-55910354 Msa0300460:five_prime_UTR 35.0%
! AGATTACTTCTTATAGTGGG+GGG + chr2_3:55908353-55908372 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_3:55908672-55908691 Msa0300460:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_3:55908709-55908728 Msa0300460:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_3:55908730-55908749 Msa0300460:CDS 35.0%
GGTGTAAATGGAAATGGATA+TGG - chr2_3:55908742-55908761 Msa0300460:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_3:55908759-55908778 Msa0300460:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_3:55908765-55908784 Msa0300460:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_3:55908778-55908797 Msa0300460:CDS 35.0%
AAAGCTTCAATACTTCTCTC+GGG + chr2_3:55908850-55908869 None:intergenic 35.0%
CAAAGCTTCAATACTTCTCT+CGG + chr2_3:55908851-55908870 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG + chr2_3:55908948-55908967 None:intergenic 35.0%
!!! GTCTAGTTTTTGTACTTCGT+AGG + chr2_3:55909171-55909190 None:intergenic 35.0%
ATTAAAGTGTGAGTCTTCAC+AGG + chr2_3:55909271-55909290 None:intergenic 35.0%
GTGAAGACTCACACTTTAAT+AGG - chr2_3:55909271-55909290 Msa0300460:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_3:55909467-55909486 Msa0300460:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_3:55909898-55909917 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_3:55909925-55909944 Msa0300460:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_3:55910214-55910233 Msa0300460:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_3:55910314-55910333 Msa0300460:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_3:55910315-55910334 Msa0300460:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_3:55910335-55910354 Msa0300460:five_prime_UTR 35.0%
TGTTGTGGTTGGAGTGTATT+TGG + chr2_3:55908480-55908499 None:intergenic 40.0%
TTGGAAGAAGAAGAAGGATG+AGG + chr2_3:55908599-55908618 None:intergenic 40.0%
ACAACGTTGGAAGAAGAAGA+AGG + chr2_3:55908605-55908624 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_3:55908618-55908637 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_3:55908651-55908670 Msa0300460:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_3:55908667-55908686 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_3:55908668-55908687 None:intergenic 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_3:55908789-55908808 Msa0300460:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_3:55908872-55908891 Msa0300460:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG - chr2_3:55908939-55908958 Msa0300460:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG + chr2_3:55908949-55908968 None:intergenic 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_3:55909298-55909317 Msa0300460:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_3:55909636-55909655 Msa0300460:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_3:55909916-55909935 Msa0300460:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_3:55910133-55910152 None:intergenic 40.0%
GGAATAGAAGTTATGTCAGC+AGG + chr2_3:55910455-55910474 None:intergenic 40.0%
TGTTGTGGTTGGAGTGTATT+TGG + chr2_3:55908480-55908499 None:intergenic 40.0%
TTGGAAGAAGAAGAAGGATG+AGG + chr2_3:55908599-55908618 None:intergenic 40.0%
ACAACGTTGGAAGAAGAAGA+AGG + chr2_3:55908605-55908624 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_3:55908618-55908637 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_3:55908651-55908670 Msa0300460:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_3:55908667-55908686 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_3:55908668-55908687 None:intergenic 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_3:55908789-55908808 Msa0300460:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_3:55908872-55908891 Msa0300460:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG - chr2_3:55908939-55908958 Msa0300460:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG + chr2_3:55908949-55908968 None:intergenic 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_3:55909298-55909317 Msa0300460:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_3:55909636-55909655 Msa0300460:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_3:55909916-55909935 Msa0300460:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_3:55910133-55910152 None:intergenic 40.0%
GGAATAGAAGTTATGTCAGC+AGG + chr2_3:55910455-55910474 None:intergenic 40.0%
!! TTTGCTGTTGCTGCTTCTTC+TGG + chr2_3:55908379-55908398 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_3:55908653-55908672 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_3:55908650-55908669 Msa0300460:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_3:55908792-55908811 Msa0300460:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_3:55908817-55908836 Msa0300460:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_3:55908875-55908894 Msa0300460:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG - chr2_3:55908928-55908947 Msa0300460:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_3:55909862-55909881 Msa0300460:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr2_3:55909863-55909882 Msa0300460:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_3:55910047-55910066 Msa0300460:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_3:55910048-55910067 Msa0300460:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_3:55910132-55910151 Msa0300460:exon 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_3:55910137-55910156 Msa0300460:exon 45.0%
!! TTTGCTGTTGCTGCTTCTTC+TGG + chr2_3:55908379-55908398 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_3:55908653-55908672 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_3:55908650-55908669 Msa0300460:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_3:55908792-55908811 Msa0300460:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_3:55908817-55908836 Msa0300460:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_3:55908875-55908894 Msa0300460:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG - chr2_3:55908928-55908947 Msa0300460:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_3:55909862-55909881 Msa0300460:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr2_3:55909863-55909882 Msa0300460:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_3:55910047-55910066 Msa0300460:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_3:55910048-55910067 Msa0300460:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_3:55910132-55910151 Msa0300460:exon 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_3:55910137-55910156 Msa0300460:exon 45.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_3:55908424-55908443 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_3:55908807-55908826 Msa0300460:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_3:55910051-55910070 Msa0300460:CDS 50.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_3:55908424-55908443 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_3:55908807-55908826 Msa0300460:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_3:55910051-55910070 Msa0300460:CDS 50.0%
TTGGTGAGATCCAGAGGCAG+AGG + chr2_3:55908418-55908437 None:intergenic 55.0%
TTGGTGAGATCCAGAGGCAG+AGG + chr2_3:55908418-55908437 None:intergenic 55.0%
ACTCTCTCTGCCTCTGCCTC+TGG - chr2_3:55908405-55908424 Msa0300460:three_prime_UTR 60.0%
ACTCTCTCTGCCTCTGCCTC+TGG - chr2_3:55908405-55908424 Msa0300460:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55908353 55910475 55908353 ID=Msa0300460;Name=Msa0300460
chr2_3 mRNA 55908353 55910475 55908353 ID=Msa0300460-mRNA-1;Parent=Msa0300460;Name=Msa0300460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=338|1|1|1|1|1|2|197|247
chr2_3 exon 55908353 55909023 55908353 ID=Msa0300460-mRNA-1:exon:17871;Parent=Msa0300460-mRNA-1
chr2_3 exon 55909868 55910475 55909868 ID=Msa0300460-mRNA-1:exon:17870;Parent=Msa0300460-mRNA-1
chr2_3 five_prime_UTR 55910138 55910475 55910138 ID=Msa0300460-mRNA-1:five_prime_utr;Parent=Msa0300460-mRNA-1
chr2_3 CDS 55909868 55910137 55909868 ID=Msa0300460-mRNA-1:cds;Parent=Msa0300460-mRNA-1
chr2_3 CDS 55908550 55909023 55908550 ID=Msa0300460-mRNA-1:cds;Parent=Msa0300460-mRNA-1
chr2_3 three_prime_UTR 55908353 55908549 55908353 ID=Msa0300460-mRNA-1:three_prime_utr;Parent=Msa0300460-mRNA-1
Gene Sequence

>Msa0300460

ATGGAAATGGATGATTTTGCTTCAAGTAGAATGAATGATGGTTGTTTTCAAGGTGTAAATGGAAATGGATATGGTTCATCTTCTTCACTGGTTTTGGATAGAGAAAAAGGAGAGCTTGTGGAGGCAGCTGTGAAATTGGAACGCAAAGGTGTGTCGCCCGAGAGAAGTATTGAAGCTTTGAAGAATCATAGTGAAGCAGAGAGGAGGAGAAGAGCAAGAATTAATTCACATCTTGATACACTTCGTAGTGTTATCCCAGGTGCTAATAAGTTGGACAAAGCATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTCATTATTCCAAAAGACAGTGATGAGATAAGAGTTGAATCACAAGAAGGTGGATTAAATGGTTACCCTTATTCAATTAGAGCATCATTATGTTGTGAATACAAACCCGGTTTATTGTCTGATATAAGAAAAGCACTTGATGCACTTCATCTTATGATAATAAGGGCAGAGATTGCAACTTTGGGAGGTAGAATGAAGAATGTCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTCTTGCTGGATCGGTTCATCAAGCTCTTAGATCTGTACTTGATAGATTTTCTGTTTCACAAGATATCTTAGAAACTAGAAAGAGAAGGAGGATTTCTATATTCAGTTCTTCATCTTTAGGTGATTTCTTATAA
Protein sequence

>Msa0300460

MEMDDFASSRMNDGCFQGVNGNGYGSSSSLVLDREKGELVEAAVKLERKGVSPERSIEALKNHSEAERRRRARINSHLDTLRSVIPGANKLDKASLLAEVITHLKELKTNAAQASEGFIIPKDSDEIRVESQEGGLNGYPYSIRASLCCEYKPGLLSDIRKALDALHLMIIRAEIATLGGRMKNVFVIISCKEQNFEDAEYRQFLAGSVHQALRSVLDRFSVSQDILETRKRRRISIFSSSSLGDFL*