AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0337980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337980 MtrunA17_Chr2g0325521 97.976 247 5 0 1 247 1 247 3.87e-179 491
Msa0337980 MtrunA17_Chr4g0041051 72.266 256 62 3 1 247 1 256 1.23e-121 346
Msa0337980 MtrunA17_Chr4g0045901 44.672 244 124 6 2 235 13 255 6.12e-51 167
Msa0337980 MtrunA17_Chr7g0255961 47.253 182 86 4 54 234 70 242 4.07e-42 144
Msa0337980 MtrunA17_Chr1g0198801 40.594 202 109 3 45 242 144 338 1.99e-34 126
Msa0337980 MtrunA17_Chr3g0113721 33.333 201 115 5 46 234 26 219 7.62e-31 114
Msa0337980 MtrunA17_Chr5g0412371 38.462 143 74 3 54 188 40 176 4.16e-26 101
Msa0337980 MtrunA17_Chr5g0427361 40.606 165 72 2 49 188 27 190 4.99e-25 99.4
Msa0337980 MtrunA17_Chr2g0283714 51.250 80 36 1 91 170 16 92 3.09e-20 83.6
Msa0337980 MtrunA17_Chr5g0411341 40.244 82 49 0 28 109 459 540 3.39e-12 65.9
Msa0337980 MtrunA17_Chr8g0366751 39.506 81 49 0 29 109 429 509 7.97e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0337980 AT1G68810.1 41.327 196 107 5 45 236 160 351 2.07e-33 124
Msa0337980 AT3G25710.1 46.622 148 70 2 45 188 118 260 9.35e-32 119
Msa0337980 AT3G56770.1 38.857 175 98 4 51 223 37 204 1.53e-30 113
Msa0337980 AT2G40200.1 39.634 164 89 3 61 219 65 223 1.65e-29 111
Msa0337980 AT2G41130.1 40.571 175 96 3 51 223 59 227 4.08e-27 105
Msa0337980 AT4G38070.1 36.154 130 76 2 59 188 92 214 1.17e-17 80.1
Msa0337980 AT5G46760.1 44.156 77 42 1 39 114 391 467 2.20e-11 63.9
Msa0337980 AT4G17880.1 40.789 76 45 0 39 114 393 468 2.30e-11 63.5
Msa0337980 AT1G32640.1 36.634 101 61 2 33 130 421 521 3.54e-11 63.2

Find 68 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCTTCTTCACTGGTTT+TGG 0.180837 2_4:+58121436 Msa0337980:CDS
CACTTCATCTTATGATAATA+AGG 0.194458 2_4:+58122699 Msa0337980:CDS
ATTGAATGATGAAAAGAAAA+AGG 0.226203 2_4:-58121189 None:intergenic
AATGATGCTCTAATTGAATA+AGG 0.233216 2_4:-58122623 None:intergenic
TGTTGGGGTTGGAGTGTATT+TGG 0.233217 2_4:-58121148 None:intergenic
CTTATCTCATCACTGTCTTT+TGG 0.234136 2_4:-58122566 None:intergenic
ATATTCAGTTCTTCATCTTT+AGG 0.245592 2_4:+58122914 Msa0337980:CDS
CCAAGAGGGTGTTCAGAATA+TGG 0.263756 2_4:-58121321 None:intergenic
TTTGCTGTTGCTGCTTCTTC+TGG 0.265344 2_4:-58121047 None:intergenic
GACTGTTATATTTGTGTGTT+TGG 0.277090 2_4:-58121237 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.279597 2_4:+58122596 Msa0337980:CDS
TTCACATGTAATGTGAATCT+TGG 0.305383 2_4:+58122969 Msa0337980:three_prime_UTR
TGTTGTTAATTTGTCATGTT+GGG 0.312268 2_4:-58121164 None:intergenic
ACAATGAATGAACTCATTCT+AGG 0.319427 2_4:+58123073 Msa0337980:three_prime_UTR
TGTGGAGGCAGCTGTGAAAT+TGG 0.360925 2_4:+58121478 Msa0337980:CDS
TGTTATCCCAGGTGCTAATA+AGG 0.365921 2_4:+58121610 Msa0337980:CDS
TGTAATAACTTCAGCTAGTA+AGG 0.369694 2_4:-58122489 None:intergenic
AAGGGCAGAGATTGCAACTT+TGG 0.372459 2_4:+58122718 Msa0337980:CDS
GTAAAGATTACTTCTTATAG+TGG 0.377802 2_4:-58121025 None:intergenic
ACTCTCTCTGCCTCTGCCTC+TGG 0.380422 2_4:+58121076 Msa0337980:five_prime_UTR
TATGATGATGATAGTGAGTT+TGG 0.389810 2_4:-58121105 None:intergenic
TTGTTGTTAATTTGTCATGT+TGG 0.399163 2_4:-58121165 None:intergenic
GAGTATCGACAGTTTCTTGC+TGG 0.401116 2_4:+58122803 Msa0337980:CDS
GGTGTAAATGGAAGTGGATA+TGG 0.413506 2_4:+58121413 Msa0337980:CDS
CAAAGCTTCAATACTTCTCT+CGG 0.423960 2_4:-58121519 None:intergenic
AGGGCAGAGATTGCAACTTT+GGG 0.424505 2_4:+58122719 Msa0337980:CDS
TCTTATATCAGACAATAAAC+CGG 0.424693 2_4:-58122663 None:intergenic
CCATATTCTGAACACCCTCT+TGG 0.428144 2_4:+58121321 Msa0337980:five_prime_UTR
ATATGGTTCATCTTCTTCAC+TGG 0.433898 2_4:+58121430 Msa0337980:CDS
TTAATTTGTCATGTTGGGGT+TGG 0.442961 2_4:-58121159 None:intergenic
ATGATGCTCTAATTGAATAA+GGG 0.448376 2_4:-58122622 None:intergenic
ATCTTGGGTTGTACATTTGT+TGG 0.451003 2_4:+58122985 Msa0337980:three_prime_UTR
TTTCAAGGTGTAAATGGAAG+TGG 0.455677 2_4:+58121407 Msa0337980:CDS
TCTTGGGTTGTACATTTGTT+GGG 0.456987 2_4:+58122986 Msa0337980:three_prime_UTR
CATATTCTGAACACCCTCTT+GGG 0.457908 2_4:+58121322 Msa0337980:five_prime_UTR
TAAAGATTACTTCTTATAGT+GGG 0.485015 2_4:-58121024 None:intergenic
ACTTCATCTTATGATAATAA+GGG 0.489015 2_4:+58122700 Msa0337980:CDS
TCGACAGTTTCTTGCTGGAT+CGG 0.489881 2_4:+58122808 Msa0337980:CDS
CTTATATCAGACAATAAACC+GGG 0.504302 2_4:-58122662 None:intergenic
TCACATGTAATGTGAATCTT+GGG 0.510126 2_4:+58122970 Msa0337980:three_prime_UTR
GAACTAACCTTATTAGCACC+TGG 0.521161 2_4:-58121617 None:intergenic
TTGGTGAGATCCAGAGGCAG+AGG 0.526086 2_4:-58121086 None:intergenic
TTATATTTGTGTGTTTGGAA+TGG 0.537796 2_4:-58121232 None:intergenic
AAAGCTTCAATACTTCTCTC+GGG 0.540932 2_4:-58121518 None:intergenic
AGCAAGAAACTGTCGATACT+CGG 0.542573 2_4:-58122801 None:intergenic
TTGGAAGAAGAAGAAGGATG+AGG 0.553860 2_4:-58121267 None:intergenic
GTTGTTAATTTGTCATGTTG+GGG 0.557500 2_4:-58121163 None:intergenic
TTATGTTGTGAATACAAACC+CGG 0.569012 2_4:+58122644 Msa0337980:CDS
GTGAGTTTGGTGAGATCCAG+AGG 0.570542 2_4:-58121092 None:intergenic
AACTAACCTTATTAGCACCT+GGG 0.573545 2_4:-58121616 None:intergenic
ACAACGTTGGAAGAAGAAGA+AGG 0.578318 2_4:-58121273 None:intergenic
AATGCAGCACAAGCAAGTGA+AGG 0.583141 2_4:+58122533 Msa0337980:CDS
AAAGATTACTTCTTATAGTG+GGG 0.583347 2_4:-58121023 None:intergenic
GCTTCAAGTAGAATGAATGA+TGG 0.589941 2_4:+58121380 Msa0337980:CDS
GCAGAGATTGCAACTTTGGG+AGG 0.592208 2_4:+58122722 Msa0337980:CDS
ATGCAGCACAAGCAAGTGAA+GGG 0.603381 2_4:+58122534 Msa0337980:CDS
ATCATAGTGAAGCAGAGAGG+AGG 0.603979 2_4:+58121546 Msa0337980:CDS
TCTTAGAAACTAGAAAGAGA+AGG 0.613686 2_4:+58122882 Msa0337980:CDS
TAGAAACTAGAAAGAGAAGG+AGG 0.614835 2_4:+58122885 Msa0337980:CDS
AAGATTACTTCTTATAGTGG+GGG 0.618050 2_4:-58121022 None:intergenic
ATAAGAGTTGAATCACAAGA+AGG 0.621854 2_4:+58122584 Msa0337980:CDS
ACACTTCGTAGTGTTATCCC+AGG 0.622939 2_4:+58121599 Msa0337980:CDS
AGAGTTGAATCACAAGAAGG+TGG 0.624818 2_4:+58122587 Msa0337980:CDS
GACACAAAGAAAGACAACGT+TGG 0.650206 2_4:-58121286 None:intergenic
AAACAACACAGTTCCCAAGA+GGG 0.661371 2_4:-58121335 None:intergenic
AAAACAACACAGTTCCCAAG+AGG 0.688172 2_4:-58121336 None:intergenic
GCTGTGAAATTGGAACGCAA+AGG 0.704659 2_4:+58121488 Msa0337980:CDS
AGAATCATAGTGAAGCAGAG+AGG 0.708784 2_4:+58121543 Msa0337980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGAATAGAATAATTTAATTT+TGG + chr2_4:58122279-58122298 Msa0337980:intron 15.0%
!!! GGAATAGAATAATTTAATTT+TGG + chr2_4:58122279-58122298 Msa0337980:intron 15.0%
!! TAAAGATTACTTCTTATAGT+GGG - chr2_4:58121027-58121046 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG - chr2_4:58121191-58121210 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG - chr2_4:58121192-58121211 None:intergenic 20.0%
!! ATTAACTCCAAATATTTGAA+GGG + chr2_4:58121737-58121756 Msa0337980:intron 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG + chr2_4:58122258-58122277 Msa0337980:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG + chr2_4:58122700-58122719 Msa0337980:CDS 20.0%
!! TAAAGATTACTTCTTATAGT+GGG - chr2_4:58121027-58121046 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG - chr2_4:58121191-58121210 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG - chr2_4:58121192-58121211 None:intergenic 20.0%
!! ATTAACTCCAAATATTTGAA+GGG + chr2_4:58121737-58121756 Msa0337980:intron 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG + chr2_4:58122258-58122277 Msa0337980:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG + chr2_4:58122700-58122719 Msa0337980:CDS 20.0%
! GTAAAGATTACTTCTTATAG+TGG - chr2_4:58121028-58121047 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG - chr2_4:58121167-58121186 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG - chr2_4:58121168-58121187 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG - chr2_4:58121235-58121254 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG + chr2_4:58121682-58121701 Msa0337980:intron 25.0%
! GATTAACTCCAAATATTTGA+AGG + chr2_4:58121736-58121755 Msa0337980:intron 25.0%
! ATTCTTTCCCTTCAAATATT+TGG - chr2_4:58121747-58121766 None:intergenic 25.0%
! ACACTTTAATAGGGAATATA+AGG + chr2_4:58121952-58121971 Msa0337980:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG - chr2_4:58121982-58122001 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG + chr2_4:58122088-58122107 Msa0337980:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG + chr2_4:58122226-58122245 Msa0337980:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG - chr2_4:58122253-58122272 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG + chr2_4:58122257-58122276 Msa0337980:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG - chr2_4:58122271-58122290 None:intergenic 25.0%
! AAGTATAAGCATAACTATGT+TGG - chr2_4:58122362-58122381 None:intergenic 25.0%
! AACATAGTTATGCTTATACT+TGG + chr2_4:58122361-58122380 Msa0337980:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG - chr2_4:58122625-58122644 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG - chr2_4:58122626-58122645 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG - chr2_4:58122666-58122685 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG + chr2_4:58122699-58122718 Msa0337980:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG + chr2_4:58122914-58122933 Msa0337980:CDS 25.0%
! AAAGATTACTTCTTATAGTG+GGG - chr2_4:58121026-58121045 None:intergenic 25.0%
! GTAAAGATTACTTCTTATAG+TGG - chr2_4:58121028-58121047 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG - chr2_4:58121167-58121186 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG - chr2_4:58121168-58121187 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG - chr2_4:58121235-58121254 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG + chr2_4:58121682-58121701 Msa0337980:intron 25.0%
! GATTAACTCCAAATATTTGA+AGG + chr2_4:58121736-58121755 Msa0337980:intron 25.0%
! ATTCTTTCCCTTCAAATATT+TGG - chr2_4:58121747-58121766 None:intergenic 25.0%
! ACACTTTAATAGGGAATATA+AGG + chr2_4:58121952-58121971 Msa0337980:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG - chr2_4:58121982-58122001 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG + chr2_4:58122088-58122107 Msa0337980:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG + chr2_4:58122226-58122245 Msa0337980:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG - chr2_4:58122253-58122272 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG + chr2_4:58122257-58122276 Msa0337980:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG - chr2_4:58122271-58122290 None:intergenic 25.0%
! AAGTATAAGCATAACTATGT+TGG - chr2_4:58122362-58122381 None:intergenic 25.0%
! AACATAGTTATGCTTATACT+TGG + chr2_4:58122361-58122380 Msa0337980:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG - chr2_4:58122625-58122644 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG - chr2_4:58122626-58122645 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG - chr2_4:58122666-58122685 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG + chr2_4:58122699-58122718 Msa0337980:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG + chr2_4:58122914-58122933 Msa0337980:CDS 25.0%
! TATGATGATGATAGTGAGTT+TGG - chr2_4:58121108-58121127 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG - chr2_4:58121166-58121185 None:intergenic 30.0%
GACTGTTATATTTGTGTGTT+TGG - chr2_4:58121240-58121259 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG + chr2_4:58121349-58121368 Msa0337980:exon 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG + chr2_4:58121392-58121411 Msa0337980:CDS 30.0%
TGAAGACTCACACTTTAATA+GGG + chr2_4:58121943-58121962 Msa0337980:intron 30.0%
CATTCAAATGACCATAAAAG+AGG - chr2_4:58121983-58122002 None:intergenic 30.0%
AGTTAAATTGAGACAGTGAA+TGG + chr2_4:58122127-58122146 Msa0337980:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG + chr2_4:58122139-58122158 Msa0337980:intron 30.0%
! AATGGCTTTACTCACATATA+TGG - chr2_4:58122167-58122186 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG - chr2_4:58122185-58122204 None:intergenic 30.0%
!! GTGTACTTTTTGAACTTAGT+TGG + chr2_4:58122457-58122476 Msa0337980:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG - chr2_4:58122492-58122511 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG + chr2_4:58122584-58122603 Msa0337980:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG + chr2_4:58122644-58122663 Msa0337980:CDS 30.0%
CTTATATCAGACAATAAACC+GGG - chr2_4:58122665-58122684 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG + chr2_4:58122882-58122901 Msa0337980:CDS 30.0%
TTCACATGTAATGTGAATCT+TGG + chr2_4:58122969-58122988 Msa0337980:three_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG + chr2_4:58122970-58122989 Msa0337980:three_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG + chr2_4:58123073-58123092 Msa0337980:three_prime_UTR 30.0%
AAGATTACTTCTTATAGTGG+GGG - chr2_4:58121025-58121044 None:intergenic 30.0%
! TATGATGATGATAGTGAGTT+TGG - chr2_4:58121108-58121127 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG - chr2_4:58121166-58121185 None:intergenic 30.0%
GACTGTTATATTTGTGTGTT+TGG - chr2_4:58121240-58121259 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG + chr2_4:58121349-58121368 Msa0337980:exon 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG + chr2_4:58121392-58121411 Msa0337980:CDS 30.0%
TGAAGACTCACACTTTAATA+GGG + chr2_4:58121943-58121962 Msa0337980:intron 30.0%
CATTCAAATGACCATAAAAG+AGG - chr2_4:58121983-58122002 None:intergenic 30.0%
AGTTAAATTGAGACAGTGAA+TGG + chr2_4:58122127-58122146 Msa0337980:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG + chr2_4:58122139-58122158 Msa0337980:intron 30.0%
! AATGGCTTTACTCACATATA+TGG - chr2_4:58122167-58122186 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG - chr2_4:58122185-58122204 None:intergenic 30.0%
!! GTGTACTTTTTGAACTTAGT+TGG + chr2_4:58122457-58122476 Msa0337980:intron 30.0%
TGTAATAACTTCAGCTAGTA+AGG - chr2_4:58122492-58122511 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG + chr2_4:58122584-58122603 Msa0337980:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG + chr2_4:58122644-58122663 Msa0337980:CDS 30.0%
CTTATATCAGACAATAAACC+GGG - chr2_4:58122665-58122684 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG + chr2_4:58122882-58122901 Msa0337980:CDS 30.0%
TTCACATGTAATGTGAATCT+TGG + chr2_4:58122969-58122988 Msa0337980:three_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG + chr2_4:58122970-58122989 Msa0337980:three_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG + chr2_4:58123073-58123092 Msa0337980:three_prime_UTR 30.0%
!! TTAATTTGTCATGTTGGGGT+TGG - chr2_4:58121162-58121181 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG + chr2_4:58121343-58121362 Msa0337980:exon 35.0%
GCTTCAAGTAGAATGAATGA+TGG + chr2_4:58121380-58121399 Msa0337980:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG + chr2_4:58121401-58121420 Msa0337980:CDS 35.0%
TTTCAAGGTGTAAATGGAAG+TGG + chr2_4:58121407-58121426 Msa0337980:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG + chr2_4:58121430-58121449 Msa0337980:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG + chr2_4:58121436-58121455 Msa0337980:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG + chr2_4:58121449-58121468 Msa0337980:CDS 35.0%
AAAGCTTCAATACTTCTCTC+GGG - chr2_4:58121521-58121540 None:intergenic 35.0%
CAAAGCTTCAATACTTCTCT+CGG - chr2_4:58121522-58121541 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG - chr2_4:58121619-58121638 None:intergenic 35.0%
!!! GTCTAGTTTTTGTACTTCGT+AGG - chr2_4:58121842-58121861 None:intergenic 35.0%
ATTAAAGTGTGAGTCTTCAC+AGG - chr2_4:58121942-58121961 None:intergenic 35.0%
GTGAAGACTCACACTTTAAT+AGG + chr2_4:58121942-58121961 Msa0337980:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG + chr2_4:58122138-58122157 Msa0337980:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG - chr2_4:58122569-58122588 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG + chr2_4:58122596-58122615 Msa0337980:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG + chr2_4:58122885-58122904 Msa0337980:CDS 35.0%
! ATCTTGGGTTGTACATTTGT+TGG + chr2_4:58122985-58123004 Msa0337980:three_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG + chr2_4:58122986-58123005 Msa0337980:three_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG + chr2_4:58123006-58123025 Msa0337980:three_prime_UTR 35.0%
! AGATTACTTCTTATAGTGGG+GGG - chr2_4:58121024-58121043 None:intergenic 35.0%
!! TTAATTTGTCATGTTGGGGT+TGG - chr2_4:58121162-58121181 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG + chr2_4:58121343-58121362 Msa0337980:exon 35.0%
GCTTCAAGTAGAATGAATGA+TGG + chr2_4:58121380-58121399 Msa0337980:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG + chr2_4:58121401-58121420 Msa0337980:CDS 35.0%
TTTCAAGGTGTAAATGGAAG+TGG + chr2_4:58121407-58121426 Msa0337980:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG + chr2_4:58121430-58121449 Msa0337980:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG + chr2_4:58121436-58121455 Msa0337980:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG + chr2_4:58121449-58121468 Msa0337980:CDS 35.0%
AAAGCTTCAATACTTCTCTC+GGG - chr2_4:58121521-58121540 None:intergenic 35.0%
CAAAGCTTCAATACTTCTCT+CGG - chr2_4:58121522-58121541 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG - chr2_4:58121619-58121638 None:intergenic 35.0%
!!! GTCTAGTTTTTGTACTTCGT+AGG - chr2_4:58121842-58121861 None:intergenic 35.0%
ATTAAAGTGTGAGTCTTCAC+AGG - chr2_4:58121942-58121961 None:intergenic 35.0%
GTGAAGACTCACACTTTAAT+AGG + chr2_4:58121942-58121961 Msa0337980:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG + chr2_4:58122138-58122157 Msa0337980:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG - chr2_4:58122569-58122588 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG + chr2_4:58122596-58122615 Msa0337980:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG + chr2_4:58122885-58122904 Msa0337980:CDS 35.0%
! ATCTTGGGTTGTACATTTGT+TGG + chr2_4:58122985-58123004 Msa0337980:three_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG + chr2_4:58122986-58123005 Msa0337980:three_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG + chr2_4:58123006-58123025 Msa0337980:three_prime_UTR 35.0%
TTGGAAGAAGAAGAAGGATG+AGG - chr2_4:58121270-58121289 None:intergenic 40.0%
ACAACGTTGGAAGAAGAAGA+AGG - chr2_4:58121276-58121295 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG - chr2_4:58121289-58121308 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG + chr2_4:58121322-58121341 Msa0337980:five_prime_UTR 40.0%
AAACAACACAGTTCCCAAGA+GGG - chr2_4:58121338-58121357 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG - chr2_4:58121339-58121358 None:intergenic 40.0%
GGTGTAAATGGAAGTGGATA+TGG + chr2_4:58121413-58121432 Msa0337980:CDS 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG + chr2_4:58121460-58121479 Msa0337980:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG + chr2_4:58121543-58121562 Msa0337980:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG + chr2_4:58121610-58121629 Msa0337980:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG - chr2_4:58121620-58121639 None:intergenic 40.0%
! ATAAGGACGACCCTCTTTTA+TGG + chr2_4:58121969-58121988 Msa0337980:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG + chr2_4:58122307-58122326 Msa0337980:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG + chr2_4:58122587-58122606 Msa0337980:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG - chr2_4:58122804-58122823 None:intergenic 40.0%
TTGGAAGAAGAAGAAGGATG+AGG - chr2_4:58121270-58121289 None:intergenic 40.0%
ACAACGTTGGAAGAAGAAGA+AGG - chr2_4:58121276-58121295 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG - chr2_4:58121289-58121308 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG + chr2_4:58121322-58121341 Msa0337980:five_prime_UTR 40.0%
AAACAACACAGTTCCCAAGA+GGG - chr2_4:58121338-58121357 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG - chr2_4:58121339-58121358 None:intergenic 40.0%
GGTGTAAATGGAAGTGGATA+TGG + chr2_4:58121413-58121432 Msa0337980:CDS 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG + chr2_4:58121460-58121479 Msa0337980:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG + chr2_4:58121543-58121562 Msa0337980:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG + chr2_4:58121610-58121629 Msa0337980:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG - chr2_4:58121620-58121639 None:intergenic 40.0%
! ATAAGGACGACCCTCTTTTA+TGG + chr2_4:58121969-58121988 Msa0337980:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG + chr2_4:58122307-58122326 Msa0337980:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG + chr2_4:58122587-58122606 Msa0337980:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG - chr2_4:58122804-58122823 None:intergenic 40.0%
!! TTTGCTGTTGCTGCTTCTTC+TGG - chr2_4:58121050-58121069 None:intergenic 45.0%
TGTTGGGGTTGGAGTGTATT+TGG - chr2_4:58121151-58121170 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG - chr2_4:58121324-58121343 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG + chr2_4:58121321-58121340 Msa0337980:five_prime_UTR 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG + chr2_4:58121463-58121482 Msa0337980:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG + chr2_4:58121488-58121507 Msa0337980:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG + chr2_4:58121546-58121565 Msa0337980:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG + chr2_4:58121599-58121618 Msa0337980:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG + chr2_4:58122533-58122552 Msa0337980:CDS 45.0%
ATGCAGCACAAGCAAGTGAA+GGG + chr2_4:58122534-58122553 Msa0337980:CDS 45.0%
AAGGGCAGAGATTGCAACTT+TGG + chr2_4:58122718-58122737 Msa0337980:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG + chr2_4:58122719-58122738 Msa0337980:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG + chr2_4:58122803-58122822 Msa0337980:CDS 45.0%
TCGACAGTTTCTTGCTGGAT+CGG + chr2_4:58122808-58122827 Msa0337980:CDS 45.0%
!! TTTGCTGTTGCTGCTTCTTC+TGG - chr2_4:58121050-58121069 None:intergenic 45.0%
TGTTGGGGTTGGAGTGTATT+TGG - chr2_4:58121151-58121170 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG - chr2_4:58121324-58121343 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG + chr2_4:58121321-58121340 Msa0337980:five_prime_UTR 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG + chr2_4:58121463-58121482 Msa0337980:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG + chr2_4:58121488-58121507 Msa0337980:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG + chr2_4:58121546-58121565 Msa0337980:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG + chr2_4:58121599-58121618 Msa0337980:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG + chr2_4:58122533-58122552 Msa0337980:CDS 45.0%
ATGCAGCACAAGCAAGTGAA+GGG + chr2_4:58122534-58122553 Msa0337980:CDS 45.0%
AAGGGCAGAGATTGCAACTT+TGG + chr2_4:58122718-58122737 Msa0337980:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG + chr2_4:58122719-58122738 Msa0337980:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG + chr2_4:58122803-58122822 Msa0337980:CDS 45.0%
TCGACAGTTTCTTGCTGGAT+CGG + chr2_4:58122808-58122827 Msa0337980:CDS 45.0%
! GTGAGTTTGGTGAGATCCAG+AGG - chr2_4:58121095-58121114 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG + chr2_4:58121478-58121497 Msa0337980:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG + chr2_4:58122722-58122741 Msa0337980:CDS 50.0%
! GTGAGTTTGGTGAGATCCAG+AGG - chr2_4:58121095-58121114 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG + chr2_4:58121478-58121497 Msa0337980:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG + chr2_4:58122722-58122741 Msa0337980:CDS 50.0%
TTGGTGAGATCCAGAGGCAG+AGG - chr2_4:58121089-58121108 None:intergenic 55.0%
TTGGTGAGATCCAGAGGCAG+AGG - chr2_4:58121089-58121108 None:intergenic 55.0%
ACTCTCTCTGCCTCTGCCTC+TGG + chr2_4:58121076-58121095 Msa0337980:five_prime_UTR 60.0%
ACTCTCTCTGCCTCTGCCTC+TGG + chr2_4:58121076-58121095 Msa0337980:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 58121024 58123146 58121024 ID=Msa0337980;Name=Msa0337980
chr2_4 mRNA 58121024 58123146 58121024 ID=Msa0337980-mRNA-1;Parent=Msa0337980;Name=Msa0337980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=338|1|1|1|1|1|2|197|247
chr2_4 exon 58121024 58121631 58121024 ID=Msa0337980-mRNA-1:exon:17779;Parent=Msa0337980-mRNA-1
chr2_4 exon 58122476 58123146 58122476 ID=Msa0337980-mRNA-1:exon:17780;Parent=Msa0337980-mRNA-1
chr2_4 five_prime_UTR 58121024 58121361 58121024 ID=Msa0337980-mRNA-1:five_prime_utr;Parent=Msa0337980-mRNA-1
chr2_4 CDS 58121362 58121631 58121362 ID=Msa0337980-mRNA-1:cds;Parent=Msa0337980-mRNA-1
chr2_4 CDS 58122476 58122949 58122476 ID=Msa0337980-mRNA-1:cds;Parent=Msa0337980-mRNA-1
chr2_4 three_prime_UTR 58122950 58123146 58122950 ID=Msa0337980-mRNA-1:three_prime_utr;Parent=Msa0337980-mRNA-1
Gene Sequence

>Msa0337980

ATGGAAATGGATGATTTTGCTTCAAGTAGAATGAATGATGGTTGTTTTCAAGGTGTAAATGGAAGTGGATATGGTTCATCTTCTTCACTGGTTTTGGATAGAGAAAAAGGAGAGCTTGTGGAGGCAGCTGTGAAATTGGAACGCAAAGGTGTGTCGCCCGAGAGAAGTATTGAAGCTTTGAAGAATCATAGTGAAGCAGAGAGGAGGAGAAGAGCAAGAATTAATTCACATCTTGATACACTTCGTAGTGTTATCCCAGGTGCTAATAAGTTGGACAAAGCATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTCATTATTCCAAAAGACAGTGATGAGATAAGAGTTGAATCACAAGAAGGTGGATTAAATGGTTACCCTTATTCAATTAGAGCATCATTATGTTGTGAATACAAACCCGGTTTATTGTCTGATATAAGAAAAGCACTTGATGCACTTCATCTTATGATAATAAGGGCAGAGATTGCAACTTTGGGAGGTAGAATGAAGAATGTCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTCTTGCTGGATCGGTTCATCAAGCTCTTAGATCTGTACTTGATAGATTTTCTGTTTCACAAGATATCTTAGAAACTAGAAAGAGAAGGAGGATTTCTATATTCAGTTCTTCATCTTTAGGTGATTTCTTATAA
Protein sequence

>Msa0337980

MEMDDFASSRMNDGCFQGVNGSGYGSSSSLVLDREKGELVEAAVKLERKGVSPERSIEALKNHSEAERRRRARINSHLDTLRSVIPGANKLDKASLLAEVITHLKELKTNAAQASEGFIIPKDSDEIRVESQEGGLNGYPYSIRASLCCEYKPGLLSDIRKALDALHLMIIRAEIATLGGRMKNVFVIISCKEQNFEDAEYRQFLAGSVHQALRSVLDRFSVSQDILETRKRRRISIFSSSSLGDFL*