AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302030 MtrunA17_Chr2g0327781 100.000 154 0 0 1 154 1 154 3.29e-107 302
Msa0302030 MtrunA17_Chr4g0030821 95.364 151 6 1 4 154 3 152 4.20e-81 236
Msa0302030 MtrunA17_Chr7g0222741 93.506 154 10 0 1 154 1 154 3.56e-71 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302030 AT3G55280.1 87.662 154 19 0 1 154 1 154 1.89e-71 212
Msa0302030 AT3G55280.2 87.662 154 19 0 1 154 1 154 1.89e-71 212
Msa0302030 AT2G39460.1 87.662 154 19 0 1 154 1 154 3.05e-68 204
Msa0302030 AT2G39460.2 87.662 154 19 0 1 154 1 154 3.05e-68 204
Msa0302030 AT3G55280.3 93.220 118 8 0 37 154 31 148 4.61e-67 201

Find 67 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGATTGGGATTATTTA+AGG 0.136899 2_3:+58585132 Msa0302030:CDS
CTTCTTTGCCTTCTTCTTGA+TGG 0.213233 2_3:-58584613 None:intergenic
GCACTAATCCGAGGGTATTT+TGG 0.229197 2_3:-58584690 None:intergenic
GATCAAGGTGTTAACTTTCT+TGG 0.259114 2_3:-58584880 None:intergenic
CAACCTTACATAGGCCTTCT+TGG 0.259824 2_3:-58585079 None:intergenic
TCACGCAGATTTCACATCTA+AGG 0.290602 2_3:+58582312 Msa0302030:five_prime_UTR
GAGAGAATAATCTAGAAATT+AGG 0.310140 2_3:-58585461 None:intergenic
GAAGAAAATGTACGACATTC+AGG 0.310259 2_3:+58584857 Msa0302030:CDS
GCTTTCAAGGCCTGTGCCTT+TGG 0.317092 2_3:-58584564 None:intergenic
AAGATCTGTGCTGTCACCTT+TGG 0.328762 2_3:+58585530 Msa0302030:three_prime_UTR
CTATCCCTAGATAAAGTCTT+TGG 0.341250 2_3:-58584663 None:intergenic
CACTGCTTTCGCAGCTTTCA+AGG 0.343655 2_3:-58584577 None:intergenic
CTTCATCTTCTCCGATTCAA+TGG 0.360156 2_3:+58582339 Msa0302030:exon
CCTACTTGCTTAGCATTATG+AGG 0.361512 2_3:-58585580 None:intergenic
CAAGATTTCCATCTCTTAAG+AGG 0.365926 2_3:+58585483 Msa0302030:three_prime_UTR
ACAGGCCAAAGACTTTATCT+AGG 0.384705 2_3:+58584658 Msa0302030:CDS
ATTGGGATTATTTAAGGTAA+TGG 0.385537 2_3:+58585138 Msa0302030:exon
AGATCTGTGCTGTCACCTTT+GGG 0.409866 2_3:+58585531 Msa0302030:three_prime_UTR
GACACCTGATTATGATGCTT+TGG 0.412386 2_3:+58585101 Msa0302030:CDS
TTGGATGTTGCAAACAAGAT+TGG 0.413392 2_3:+58585120 Msa0302030:CDS
CACAATAACAAAACACAATC+CGG 0.420029 2_3:-58585249 None:intergenic
TAATCTAGAAATTAGGTAGA+TGG 0.421336 2_3:-58585454 None:intergenic
TGGATGTTGCAAACAAGATT+GGG 0.427718 2_3:+58585121 Msa0302030:CDS
ATAGGCCTTCTTGGTGCCAT+CGG 0.428001 2_3:-58585070 None:intergenic
AGAAGAAGGCAAAGAAGATC+AGG 0.439428 2_3:+58584619 Msa0302030:CDS
TTTGACGCAAAGTTAAATTC+CGG 0.442084 2_3:+58585230 Msa0302030:three_prime_UTR
CAAGGCCTGTGCCTTTGGAT+CGG 0.452797 2_3:-58584559 None:intergenic
GGATTATTTAAGGTAATGGC+TGG 0.458048 2_3:+58585142 Msa0302030:exon
CACGCAGATTTCACATCTAA+GGG 0.458062 2_3:+58582313 Msa0302030:five_prime_UTR
ACATCCAAAGCATCATAATC+AGG 0.461568 2_3:-58585105 None:intergenic
GCTGCGAAAGCAGTGAAGTC+AGG 0.467790 2_3:+58584585 Msa0302030:CDS
AGAAATTAGGTAGATGGCTT+AGG 0.472362 2_3:-58585448 None:intergenic
GATGGCTTAGGCTGAGTTGA+TGG 0.487168 2_3:-58585436 None:intergenic
ATGCTAAGCAAGTAGGCAAA+AGG 0.489058 2_3:+58585587 Msa0302030:three_prime_UTR
GATTCAATGGCTCCACCAAA+AGG 0.493121 2_3:+58582352 Msa0302030:exon
TAGGCCTTCTTGGTGCCATC+GGG 0.494781 2_3:-58585069 None:intergenic
TAATGATCAAGTTTGTTCCT+TGG 0.498760 2_3:-58584717 None:intergenic
AGAAAGTTAACACCTTGATC+AGG 0.514403 2_3:+58584883 Msa0302030:CDS
GATTGAAGACAACAACACTT+TGG 0.518542 2_3:+58584785 Msa0302030:CDS
CAAAGACTTTATCTAGGGAT+AGG 0.519733 2_3:+58584664 Msa0302030:CDS
CCTAAAGGCTTACCTGATCA+AGG 0.532987 2_3:-58584895 None:intergenic
AACAAAGTCCTCTTAAGAGA+TGG 0.534286 2_3:-58585491 None:intergenic
CAGGCCCGATGGCACCAAGA+AGG 0.551305 2_3:+58585065 Msa0302030:intron
GCACCAAGAAGGCCTATGTA+AGG 0.556146 2_3:+58585076 Msa0302030:CDS
AGGCACTGCCATCAAGAAGA+AGG 0.565573 2_3:+58584605 Msa0302030:CDS
CAGGCCAAAGACTTTATCTA+GGG 0.565675 2_3:+58584659 Msa0302030:CDS
GAAGACCGATCCAAAGGCAC+AGG 0.567558 2_3:+58584554 Msa0302030:CDS
ACTGTCTGCTTCCACCACAC+AGG 0.577415 2_3:+58582287 Msa0302030:five_prime_UTR
CCTCATAATGCTAAGCAAGT+AGG 0.580047 2_3:+58585580 Msa0302030:three_prime_UTR
TAGATAAAGTCTTTGGCCTG+TGG 0.590574 2_3:-58584656 None:intergenic
ATGTGCTATGAACATAATTG+AGG 0.592048 2_3:+58585387 Msa0302030:three_prime_UTR
GGACAACTGTTACATTCCAC+AGG 0.605601 2_3:+58584640 Msa0302030:CDS
TGCAAAGAAGACCGATCCAA+AGG 0.612710 2_3:+58584548 Msa0302030:CDS
CCTTGGTATATATCTCCCAA+AGG 0.625072 2_3:-58585546 None:intergenic
ATAGGAATCCAAAATACCCT+CGG 0.629797 2_3:+58584682 Msa0302030:CDS
ATCAGGTGTCAACCTTACAT+AGG 0.633043 2_3:-58585088 None:intergenic
ATTGCTGACTCAGTTGTGAG+CGG 0.651573 2_3:-58584756 None:intergenic
TTGGTGTAGCACTAATCCGA+GGG 0.658734 2_3:-58584698 None:intergenic
CTTGGTGTAGCACTAATCCG+AGG 0.662347 2_3:-58584699 None:intergenic
ACGCAGATTTCACATCTAAG+GGG 0.669567 2_3:+58582314 Msa0302030:five_prime_UTR
TCTGCGTGAAACCTGTGTGG+TGG 0.673550 2_3:-58582298 None:intergenic
GCGTCAAAATGTCTCCACAA+TGG 0.680459 2_3:-58585215 None:intergenic
CCTTTGGGAGATATATACCA+AGG 0.707867 2_3:+58585546 Msa0302030:three_prime_UTR
CTCGGATTAGTGCTACACCA+AGG 0.713270 2_3:+58584700 Msa0302030:CDS
GCAAGTAGGCAAAAGGACTG+CGG 0.713487 2_3:+58585594 Msa0302030:three_prime_UTR
AAATCTGCGTGAAACCTGTG+TGG 0.746608 2_3:-58582301 None:intergenic
TGTGTGGTGGAAGCAGACAG+TGG 0.760336 2_3:-58582285 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTAATATTTATTTTTTGA+AGG + chr2_3:58583395-58583414 Msa0302030:intron 10.0%
!!! TATTTTTCCTTTTTTTTAAA+AGG - chr2_3:58583514-58583533 None:intergenic 10.0%
!!! AGTAGAACTTAAAATTTTAT+AGG + chr2_3:58582786-58582805 Msa0302030:intron 15.0%
!!! TTATTTTGGTTTTGTTAAAA+TGG + chr2_3:58583158-58583177 Msa0302030:intron 15.0%
!!! CATATTTCCTTTTAAAAAAA+AGG + chr2_3:58583504-58583523 Msa0302030:intron 15.0%
!!! TTTAGATGTTAGTTTTTTTA+TGG + chr2_3:58585186-58585205 Msa0302030:three_prime_UTR 15.0%
!!! GATTATATAACAATTTTAGC+TGG - chr2_3:58582582-58582601 None:intergenic 20.0%
!! ATATAGCAAAAGATCTAAAT+TGG - chr2_3:58582756-58582775 None:intergenic 20.0%
!! ATAATAAAGAAATGAGAATG+CGG - chr2_3:58582855-58582874 None:intergenic 20.0%
!! TCTTCTAACCTAAAAAAATA+GGG + chr2_3:58583588-58583607 Msa0302030:intron 20.0%
!!! TATATTCTATCTAATTTGTG+TGG + chr2_3:58583828-58583847 Msa0302030:intron 20.0%
!!! TTTTGTTGAATAGAAGATTT+AGG + chr2_3:58584155-58584174 Msa0302030:intron 20.0%
!!! AGCTATTTTGTATACATAAA+AGG - chr2_3:58584457-58584476 None:intergenic 20.0%
!!! TTTTTCCTTTTTCTTTTTGT+AGG + chr2_3:58584519-58584538 Msa0302030:intron 20.0%
!! CTCATGTTATTATTTACAAT+GGG - chr2_3:58584994-58585013 None:intergenic 20.0%
!! TCTCATGTTATTATTTACAA+TGG - chr2_3:58584995-58585014 None:intergenic 20.0%
!!! AGTTCTTTTGCAATTAAATT+TGG + chr2_3:58585278-58585297 Msa0302030:three_prime_UTR 20.0%
!! TAGATAGATGAAATACCTTT+TGG - chr2_3:58582370-58582389 None:intergenic 25.0%
! TAATAAAGAAATGAGAATGC+GGG - chr2_3:58582854-58582873 None:intergenic 25.0%
!!! GTTTTGATTAAAAAAGTTGC+TGG + chr2_3:58583117-58583136 Msa0302030:intron 25.0%
!! AATATGTAAGACAGAAGTAT+AGG - chr2_3:58583490-58583509 None:intergenic 25.0%
! AAGAAATAAAACTACAAGTC+TGG + chr2_3:58583541-58583560 Msa0302030:intron 25.0%
! CTCTTCTAACCTAAAAAAAT+AGG + chr2_3:58583587-58583606 Msa0302030:intron 25.0%
!! AGTACTTATGATGTTCTTTT+TGG + chr2_3:58583784-58583803 Msa0302030:intron 25.0%
! AATAAGTTGTTTATCCAAAC+AGG + chr2_3:58584430-58584449 Msa0302030:intron 25.0%
! ATAAAAAGATCACAACCTAA+AGG - chr2_3:58584913-58584932 None:intergenic 25.0%
!!! TTTAGGTTGTGATCTTTTTA+TGG + chr2_3:58584912-58584931 Msa0302030:intron 25.0%
! AAACTGCTTTACAATAATGT+GGG - chr2_3:58585024-58585043 None:intergenic 25.0%
! TAAACTGCTTTACAATAATG+TGG - chr2_3:58585025-58585044 None:intergenic 25.0%
! AACAAGATTGGGATTATTTA+AGG + chr2_3:58585132-58585151 Msa0302030:CDS 25.0%
!! ATTGGGATTATTTAAGGTAA+TGG + chr2_3:58585138-58585157 Msa0302030:exon 25.0%
! ATCTAAACAAAAACTGTCTT+TGG - chr2_3:58585173-58585192 None:intergenic 25.0%
! AAATCTAAAAACAAACTTCG+AGG - chr2_3:58585343-58585362 None:intergenic 25.0%
!!! TCGAAGTTTGTTTTTAGATT+TGG + chr2_3:58585342-58585361 Msa0302030:three_prime_UTR 25.0%
!!! AAGTTTGTTTTTAGATTTGG+TGG + chr2_3:58585345-58585364 Msa0302030:three_prime_UTR 25.0%
! TAATCTAGAAATTAGGTAGA+TGG - chr2_3:58585457-58585476 None:intergenic 25.0%
! GAGAGAATAATCTAGAAATT+AGG - chr2_3:58585464-58585483 None:intergenic 25.0%
! ATAGATGAAATACCTTTTGG+TGG - chr2_3:58582367-58582386 None:intergenic 30.0%
ATGAAAACAGATGCAGAAAT+TGG - chr2_3:58582445-58582464 None:intergenic 30.0%
!! GTTGTTTTCAGAAGCAATTT+TGG - chr2_3:58582716-58582735 None:intergenic 30.0%
GACCTTGAAAGAAATAATCA+TGG + chr2_3:58582924-58582943 Msa0302030:intron 30.0%
! CTCCATGATTATTTCTTTCA+AGG - chr2_3:58582929-58582948 None:intergenic 30.0%
!!! TGATTTGCTTGGACTTATTT+TGG + chr2_3:58583144-58583163 Msa0302030:intron 30.0%
ATCTCCATGATTATTGCTTT+CGG + chr2_3:58583196-58583215 Msa0302030:intron 30.0%
! ATACGATACTTCACCATTTT+TGG - chr2_3:58583240-58583259 None:intergenic 30.0%
!!! ACGAGAAACCCTATTTTTTT+AGG - chr2_3:58583599-58583618 None:intergenic 30.0%
AGAGAATAACTATTGCTATG+TGG - chr2_3:58583624-58583643 None:intergenic 30.0%
TAGAATTCAATCAGTGGTAA+TGG - chr2_3:58583654-58583673 None:intergenic 30.0%
! CTTATGATGTTCTTTTTGGA+TGG + chr2_3:58583788-58583807 Msa0302030:intron 30.0%
!! TAGAAGATTTAGGACCTTTT+TGG + chr2_3:58584165-58584184 Msa0302030:intron 30.0%
!!! ATTTGTTTTGTGAGACTGTT+TGG + chr2_3:58584216-58584235 Msa0302030:intron 30.0%
!!! TTTGTTTTGTGAGACTGTTT+GGG + chr2_3:58584217-58584236 Msa0302030:intron 30.0%
TAAGCTATTCTTGTGCAAAT+GGG - chr2_3:58584368-58584387 None:intergenic 30.0%
ATAAGCTATTCTTGTGCAAA+TGG - chr2_3:58584369-58584388 None:intergenic 30.0%
TATACATAAAAGGTCCTGTT+TGG - chr2_3:58584447-58584466 None:intergenic 30.0%
CATCACCTACAAAAAGAAAA+AGG - chr2_3:58584527-58584546 None:intergenic 30.0%
! CTTTTTCTTTTTGTAGGTGA+TGG + chr2_3:58584525-58584544 Msa0302030:intron 30.0%
! TAATGATCAAGTTTGTTCCT+TGG - chr2_3:58584720-58584739 None:intergenic 30.0%
AGCTGACAAAAAGAAAATCA+AGG + chr2_3:58584827-58584846 Msa0302030:CDS 30.0%
! AATGTCGTACATTTTCTTCA+CGG - chr2_3:58584856-58584875 None:intergenic 30.0%
TCATATATCCCTTCACAATA+GGG - chr2_3:58584958-58584977 None:intergenic 30.0%
ATCATATATCCCTTCACAAT+AGG - chr2_3:58584959-58584978 None:intergenic 30.0%
TTTGACGCAAAGTTAAATTC+CGG + chr2_3:58585230-58585249 Msa0302030:three_prime_UTR 30.0%
CACAATAACAAAACACAATC+CGG - chr2_3:58585252-58585271 None:intergenic 30.0%
ATGTGCTATGAACATAATTG+AGG + chr2_3:58585387-58585406 Msa0302030:three_prime_UTR 30.0%
! CGAGTTTCTGGATTAACTAT+TGG + chr2_3:58582501-58582520 Msa0302030:intron 35.0%
!!! TTCACCATTTTTGGAGTATC+GGG - chr2_3:58583231-58583250 None:intergenic 35.0%
!!! CTTCACCATTTTTGGAGTAT+CGG - chr2_3:58583232-58583251 None:intergenic 35.0%
! TACTTTTCCGATACGAGTAT+CGG - chr2_3:58583289-58583308 None:intergenic 35.0%
! TACTCGTATCGGAAAAGTAT+CGG + chr2_3:58583290-58583309 Msa0302030:intron 35.0%
AAACAATGACCAATACGTGT+CGG + chr2_3:58583336-58583355 Msa0302030:intron 35.0%
AAAAAATGACCGATACGTGT+CGG + chr2_3:58583419-58583438 Msa0302030:intron 35.0%
ACAGAGTAGAATTCAATCAG+TGG - chr2_3:58583660-58583679 None:intergenic 35.0%
TCAAAATTCCCGATACGATA+CGG - chr2_3:58583992-58584011 None:intergenic 35.0%
GATACGATACGATACCAATA+CGG - chr2_3:58584031-58584050 None:intergenic 35.0%
! GTATTGGTATCGTATCGTAT+CGG + chr2_3:58584030-58584049 Msa0302030:intron 35.0%
! AATGAAGCATCCAGAGAAAT+GGG - chr2_3:58584105-58584124 None:intergenic 35.0%
! AAATGAAGCATCCAGAGAAA+TGG - chr2_3:58584106-58584125 None:intergenic 35.0%
!! TCTGGATGCTTCATTTTTCT+AGG + chr2_3:58584110-58584129 Msa0302030:intron 35.0%
!! GATTTAGGACCTTTTTGGAT+TGG + chr2_3:58584170-58584189 Msa0302030:intron 35.0%
TGAAAGAAGCCAATCCAAAA+AGG - chr2_3:58584182-58584201 None:intergenic 35.0%
! AGACTGTTTGGGAAAAGTTT+TGG + chr2_3:58584228-58584247 Msa0302030:intron 35.0%
! GACTGTTTGGGAAAAGTTTT+GGG + chr2_3:58584229-58584248 Msa0302030:intron 35.0%
CTATCCCTAGATAAAGTCTT+TGG - chr2_3:58584666-58584685 None:intergenic 35.0%
CAAAGACTTTATCTAGGGAT+AGG + chr2_3:58584664-58584683 Msa0302030:CDS 35.0%
ATAGGAATCCAAAATACCCT+CGG + chr2_3:58584682-58584701 Msa0302030:CDS 35.0%
GATTGAAGACAACAACACTT+TGG + chr2_3:58584785-58584804 Msa0302030:CDS 35.0%
GAAGAAAATGTACGACATTC+AGG + chr2_3:58584857-58584876 Msa0302030:CDS 35.0%
! GATCAAGGTGTTAACTTTCT+TGG - chr2_3:58584883-58584902 None:intergenic 35.0%
AGAAAGTTAACACCTTGATC+AGG + chr2_3:58584883-58584902 Msa0302030:CDS 35.0%
! AACTTTGCATGTTTTCTTGC+AGG + chr2_3:58585046-58585065 Msa0302030:intron 35.0%
! ACATCCAAAGCATCATAATC+AGG - chr2_3:58585108-58585127 None:intergenic 35.0%
TTGGATGTTGCAAACAAGAT+TGG + chr2_3:58585120-58585139 Msa0302030:CDS 35.0%
TGGATGTTGCAAACAAGATT+GGG + chr2_3:58585121-58585140 Msa0302030:CDS 35.0%
! GGATTATTTAAGGTAATGGC+TGG + chr2_3:58585142-58585161 Msa0302030:exon 35.0%
! TTTTATGGTGATCACCATTG+TGG + chr2_3:58585201-58585220 Msa0302030:three_prime_UTR 35.0%
AGAAATTAGGTAGATGGCTT+AGG - chr2_3:58585451-58585470 None:intergenic 35.0%
CAAGATTTCCATCTCTTAAG+AGG + chr2_3:58585483-58585502 Msa0302030:three_prime_UTR 35.0%
AACAAAGTCCTCTTAAGAGA+TGG - chr2_3:58585494-58585513 None:intergenic 35.0%
TCACGCAGATTTCACATCTA+AGG + chr2_3:58582312-58582331 Msa0302030:five_prime_UTR 40.0%
CACGCAGATTTCACATCTAA+GGG + chr2_3:58582313-58582332 Msa0302030:five_prime_UTR 40.0%
ACGCAGATTTCACATCTAAG+GGG + chr2_3:58582314-58582333 Msa0302030:five_prime_UTR 40.0%
CTTCATCTTCTCCGATTCAA+TGG + chr2_3:58582339-58582358 Msa0302030:exon 40.0%
! TTTTGGTGGAGCCATTGAAT+CGG - chr2_3:58582353-58582372 None:intergenic 40.0%
GCGGGTTTAGCAAACAAATA+AGG - chr2_3:58582836-58582855 None:intergenic 40.0%
!! TTGCTGGAACATGATTTGCT+TGG + chr2_3:58583133-58583152 Msa0302030:intron 40.0%
AGAGCCGAAAGCAATAATCA+TGG - chr2_3:58583203-58583222 None:intergenic 40.0%
!!! TCACCATTTTTGGAGTATCG+GGG - chr2_3:58583230-58583249 None:intergenic 40.0%
! ACTTTTCCGATACGAGTATC+GGG - chr2_3:58583288-58583307 None:intergenic 40.0%
ATACTTCCCGATACGCATAT+CGG + chr2_3:58583359-58583378 Msa0302030:intron 40.0%
TACTTCTCCGATATGCGTAT+CGG - chr2_3:58583369-58583388 None:intergenic 40.0%
! TACGCATATCGGAGAAGTAT+CGG + chr2_3:58583370-58583389 Msa0302030:intron 40.0%
TTAGAGTCATCCCATTTCTC+TGG + chr2_3:58584092-58584111 Msa0302030:intron 40.0%
GCTTCTTTCAGCTTATCTAC+CGG + chr2_3:58584192-58584211 Msa0302030:intron 40.0%
CAGTCTCACAAAACAAATGC+CGG - chr2_3:58584214-58584233 None:intergenic 40.0%
ATGTTTCCACGAGTTACGTT+GGG - chr2_3:58584308-58584327 None:intergenic 40.0%
TTGCTGCATGAAATGTGAGT+AGG + chr2_3:58584491-58584510 Msa0302030:intron 40.0%
CTTCTTTGCCTTCTTCTTGA+TGG - chr2_3:58584616-58584635 None:intergenic 40.0%
AGAAGAAGGCAAAGAAGATC+AGG + chr2_3:58584619-58584638 Msa0302030:CDS 40.0%
TAGATAAAGTCTTTGGCCTG+TGG - chr2_3:58584659-58584678 None:intergenic 40.0%
ACAGGCCAAAGACTTTATCT+AGG + chr2_3:58584658-58584677 Msa0302030:CDS 40.0%
CAGGCCAAAGACTTTATCTA+GGG + chr2_3:58584659-58584678 Msa0302030:CDS 40.0%
ATGGCACACTGTTATGATCA+CGG + chr2_3:58584931-58584950 Msa0302030:intron 40.0%
ATCAGGTGTCAACCTTACAT+AGG - chr2_3:58585091-58585110 None:intergenic 40.0%
GACACCTGATTATGATGCTT+TGG + chr2_3:58585101-58585120 Msa0302030:CDS 40.0%
CCTTGGTATATATCTCCCAA+AGG - chr2_3:58585549-58585568 None:intergenic 40.0%
! CCTTTGGGAGATATATACCA+AGG + chr2_3:58585546-58585565 Msa0302030:three_prime_UTR 40.0%
! ATGAGGTGATCGTTTTACCT+TGG - chr2_3:58585566-58585585 None:intergenic 40.0%
CCTACTTGCTTAGCATTATG+AGG - chr2_3:58585583-58585602 None:intergenic 40.0%
CCTCATAATGCTAAGCAAGT+AGG + chr2_3:58585580-58585599 Msa0302030:three_prime_UTR 40.0%
ATGCTAAGCAAGTAGGCAAA+AGG + chr2_3:58585587-58585606 Msa0302030:three_prime_UTR 40.0%
AAATCTGCGTGAAACCTGTG+TGG - chr2_3:58582304-58582323 None:intergenic 45.0%
GATTCAATGGCTCCACCAAA+AGG + chr2_3:58582352-58582371 Msa0302030:exon 45.0%
AAGCCCCGATACTCCAAAAA+TGG + chr2_3:58583224-58583243 Msa0302030:intron 45.0%
!! ATACGAGTATCGGGACGTAT+CGG - chr2_3:58583279-58583298 None:intergenic 45.0%
ATACGTCCCGATACTCGTAT+CGG + chr2_3:58583279-58583298 Msa0302030:intron 45.0%
ACTTCTCCGATATGCGTATC+GGG - chr2_3:58583368-58583387 None:intergenic 45.0%
!! ACGCATATCGGAGAAGTATC+GGG + chr2_3:58583371-58583390 Msa0302030:intron 45.0%
TAGTCGTATCCGACACGTAT+CGG - chr2_3:58583431-58583450 None:intergenic 45.0%
GTATCGAAGCCGTATCGTAT+CGG + chr2_3:58583980-58583999 Msa0302030:intron 45.0%
TATCGAAGCCGTATCGTATC+GGG + chr2_3:58583981-58584000 Msa0302030:intron 45.0%
! ATTTTCCCGTATCGCCGTAT+TGG + chr2_3:58584014-58584033 Msa0302030:intron 45.0%
ACGATACCAATACGGCGATA+CGG - chr2_3:58584023-58584042 None:intergenic 45.0%
! TATCGTATCGGTATCGTGTC+AGG + chr2_3:58584042-58584061 Msa0302030:intron 45.0%
ATCGGTATCGTGTCAGGTAT+CGG + chr2_3:58584048-58584067 Msa0302030:intron 45.0%
TGTTTCCACGAGTTACGTTG+GGG - chr2_3:58584307-58584326 None:intergenic 45.0%
GATGTTTCCACGAGTTACGT+TGG - chr2_3:58584309-58584328 None:intergenic 45.0%
TGCAAAGAAGACCGATCCAA+AGG + chr2_3:58584548-58584567 Msa0302030:CDS 45.0%
GGACAACTGTTACATTCCAC+AGG + chr2_3:58584640-58584659 Msa0302030:CDS 45.0%
!!! GCACTAATCCGAGGGTATTT+TGG - chr2_3:58584693-58584712 None:intergenic 45.0%
TTGGTGTAGCACTAATCCGA+GGG - chr2_3:58584701-58584720 None:intergenic 45.0%
!! ATTGCTGACTCAGTTGTGAG+CGG - chr2_3:58584759-58584778 None:intergenic 45.0%
CCTAAAGGCTTACCTGATCA+AGG - chr2_3:58584898-58584917 None:intergenic 45.0%
CCTTGATCAGGTAAGCCTTT+AGG + chr2_3:58584895-58584914 Msa0302030:intron 45.0%
TGGCACACTGTTATGATCAC+GGG + chr2_3:58584932-58584951 Msa0302030:intron 45.0%
CAACCTTACATAGGCCTTCT+TGG - chr2_3:58585082-58585101 None:intergenic 45.0%
GCGTCAAAATGTCTCCACAA+TGG - chr2_3:58585218-58585237 None:intergenic 45.0%
AAGATCTGTGCTGTCACCTT+TGG + chr2_3:58585530-58585549 Msa0302030:three_prime_UTR 45.0%
AGATCTGTGCTGTCACCTTT+GGG + chr2_3:58585531-58585550 Msa0302030:three_prime_UTR 45.0%
TCAGTGCTTGCACGAGTTTC+TGG + chr2_3:58582489-58582508 Msa0302030:intron 50.0%
!! TACGAGTATCGGGACGTATC+GGG - chr2_3:58583278-58583297 None:intergenic 50.0%
!! GGGAAGTATCCGACACGTAT+TGG - chr2_3:58583348-58583367 None:intergenic 50.0%
CGATACCAATACGGCGATAC+GGG - chr2_3:58584022-58584041 None:intergenic 50.0%
TCGGTATCGTGTCAGGTATC+GGG + chr2_3:58584049-58584068 Msa0302030:intron 50.0%
ATCGGGGCTTCATAGGCTTT+CGG + chr2_3:58584066-58584085 Msa0302030:intron 50.0%
GTTTCCACGAGTTACGTTGG+GGG - chr2_3:58584306-58584325 None:intergenic 50.0%
CACTGCTTTCGCAGCTTTCA+AGG - chr2_3:58584580-58584599 None:intergenic 50.0%
! AGGCACTGCCATCAAGAAGA+AGG + chr2_3:58584605-58584624 Msa0302030:CDS 50.0%
! CTTGGTGTAGCACTAATCCG+AGG - chr2_3:58584702-58584721 None:intergenic 50.0%
!! CTCGGATTAGTGCTACACCA+AGG + chr2_3:58584700-58584719 Msa0302030:CDS 50.0%
ATCACGGGCCCTATTGTGAA+GGG + chr2_3:58584947-58584966 Msa0302030:intron 50.0%
! ATGTTTTCTTGCAGGCCCGA+TGG + chr2_3:58585054-58585073 Msa0302030:intron 50.0%
!! ATAGGCCTTCTTGGTGCCAT+CGG - chr2_3:58585073-58585092 None:intergenic 50.0%
GCACCAAGAAGGCCTATGTA+AGG + chr2_3:58585076-58585095 Msa0302030:CDS 50.0%
! GATGGCTTAGGCTGAGTTGA+TGG - chr2_3:58585439-58585458 None:intergenic 50.0%
GCAAGTAGGCAAAAGGACTG+CGG + chr2_3:58585594-58585613 Msa0302030:three_prime_UTR 50.0%
! TGTGTGGTGGAAGCAGACAG+TGG - chr2_3:58582288-58582307 None:intergenic 55.0%
ACTGTCTGCTTCCACCACAC+AGG + chr2_3:58582287-58582306 Msa0302030:five_prime_UTR 55.0%
TCTGCGTGAAACCTGTGTGG+TGG - chr2_3:58582301-58582320 None:intergenic 55.0%
! GTATCGGGGCGTATCGGTAT+CGG - chr2_3:58583263-58583282 None:intergenic 55.0%
!! ACGAGTATCGGGACGTATCG+GGG - chr2_3:58583277-58583296 None:intergenic 55.0%
! CGGTATCGTGTCAGGTATCG+GGG + chr2_3:58584050-58584069 Msa0302030:intron 55.0%
!! GTCAGGTATCGGGGCTTCAT+AGG + chr2_3:58584059-58584078 Msa0302030:intron 55.0%
AGTTCCCCCAACGTAACTCG+TGG + chr2_3:58584299-58584318 Msa0302030:intron 55.0%
! GAAGACCGATCCAAAGGCAC+AGG + chr2_3:58584554-58584573 Msa0302030:CDS 55.0%
CAAGGCCTGTGCCTTTGGAT+CGG - chr2_3:58584562-58584581 None:intergenic 55.0%
GCTTTCAAGGCCTGTGCCTT+TGG - chr2_3:58584567-58584586 None:intergenic 55.0%
GCTGCGAAAGCAGTGAAGTC+AGG + chr2_3:58584585-58584604 Msa0302030:CDS 55.0%
GATCACGGGCCCTATTGTGA+AGG + chr2_3:58584946-58584965 Msa0302030:intron 55.0%
!! TAGGCCTTCTTGGTGCCATC+GGG - chr2_3:58585072-58585091 None:intergenic 55.0%
CGGGACGTATCGGGGCGTAT+CGG - chr2_3:58583269-58583288 None:intergenic 65.0%
CAGGCCCGATGGCACCAAGA+AGG + chr2_3:58585065-58585084 Msa0302030:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 58582281 58585638 58582281 ID=Msa0302030;Name=Msa0302030
chr2_3 mRNA 58582281 58585638 58582281 ID=Msa0302030-mRNA-1;Parent=Msa0302030;Name=Msa0302030-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77|1|1|1|1|1|3|486|154
chr2_3 exon 58582281 58582373 58582281 ID=Msa0302030-mRNA-1:exon:18897;Parent=Msa0302030-mRNA-1
chr2_3 exon 58584541 58584904 58584541 ID=Msa0302030-mRNA-1:exon:18898;Parent=Msa0302030-mRNA-1
chr2_3 exon 58585068 58585638 58585068 ID=Msa0302030-mRNA-1:exon:18899;Parent=Msa0302030-mRNA-1
chr2_3 five_prime_UTR 58582281 58582357 58582281 ID=Msa0302030-mRNA-1:five_prime_utr;Parent=Msa0302030-mRNA-1
chr2_3 CDS 58582358 58582373 58582358 ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3 CDS 58584541 58584904 58584541 ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3 CDS 58585068 58585152 58585068 ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3 three_prime_UTR 58585153 58585638 58585153 ID=Msa0302030-mRNA-1:three_prime_utr;Parent=Msa0302030-mRNA-1
Gene Sequence

>Msa0302030

ATGGCTCCACCAAAAGGTGATGGTGCAAAGAAGACCGATCCAAAGGCACAGGCCTTGAAAGCTGCGAAAGCAGTGAAGTCAGGCACTGCCATCAAGAAGAAGGCAAAGAAGATCAGGACAACTGTTACATTCCACAGGCCAAAGACTTTATCTAGGGATAGGAATCCAAAATACCCTCGGATTAGTGCTACACCAAGGAACAAACTTGATCATTATCAGATTTTGAAGTATCCGCTCACAACTGAGTCAGCAATGAAGAAGATTGAAGACAACAACACTTTGGTTTTCATTGTTGACTTGCGAGCTGACAAAAAGAAAATCAAGGATGCCGTGAAGAAAATGTACGACATTCAGGCCAAGAAAGTTAACACCTTGATCAGGCCCGATGGCACCAAGAAGGCCTATGTAAGGTTGACACCTGATTATGATGCTTTGGATGTTGCAAACAAGATTGGGATTATTTAA
Protein sequence

>Msa0302030

MAPPKGDGAKKTDPKAQALKAAKAVKSGTAIKKKAKKIRTTVTFHRPKTLSRDRNPKYPRISATPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII*