AlfalfaGEDB | Msa0302030

M. sativa cultivar zhongmu-4 / Msa0302030

KEGG: K02893
GO: -
PFAM: PF03939.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0302030	XP_003597322.1	100.000	154	0	0	1	154	1	154	3.58e-103	302

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0302030	sp\|Q9AT35\|RL23A_DAUCA	87.013	154	20	0	1	154	1	154	1.71e-83	245

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0302030	B7FGR0	100.000	154	0	0	1	154	1	154	1.71e-103	302

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0302030	Msa0781310	0.807112	5.927121e-50	-8.615850e-47
Msa0302030	Msa1240270	0.805493	1.299743e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0302030	MtrunA17_Chr2g0327781	100.000	154	0	0	1	154	1	154	3.29e-107	302
Msa0302030	MtrunA17_Chr4g0030821	95.364	151	6	1	4	154	3	152	4.20e-81	236
Msa0302030	MtrunA17_Chr7g0222741	93.506	154	10	0	1	154	1	154	3.56e-71	211

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0302030	AT3G55280.1	87.662	154	19	1	154	1	154	1.89e-71	212
Msa0302030	AT3G55280.2	87.662	154	19	1	154	1	154	1.89e-71	212
Msa0302030	AT2G39460.1	87.662	154	19	1	154	1	154	3.05e-68	204
Msa0302030	AT2G39460.2	87.662	154	19	1	154	1	154	3.05e-68	204
Msa0302030	AT3G55280.3	93.220	118	8	37	154	31	148	4.61e-67	201

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Find 67 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTGCGTGAAACCTGTG+TGG	0.746608	2_3:-58582301	None:intergenic
AACAAAGTCCTCTTAAGAGA+TGG	0.534286	2_3:-58585491	None:intergenic
AACAAGATTGGGATTATTTA+AGG	0.136899	2_3:+58585132	Msa0302030:CDS
AAGATCTGTGCTGTCACCTT+TGG	0.328762	2_3:+58585530	Msa0302030:three_prime_UTR
ACAGGCCAAAGACTTTATCT+AGG	0.384705	2_3:+58584658	Msa0302030:CDS
ACATCCAAAGCATCATAATC+AGG	0.461568	2_3:-58585105	None:intergenic
ACGCAGATTTCACATCTAAG+GGG	0.669567	2_3:+58582314	Msa0302030:five_prime_UTR
ACTGTCTGCTTCCACCACAC+AGG	0.577415	2_3:+58582287	Msa0302030:five_prime_UTR
AGAAAGTTAACACCTTGATC+AGG	0.514403	2_3:+58584883	Msa0302030:CDS
AGAAATTAGGTAGATGGCTT+AGG	0.472362	2_3:-58585448	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAAAACAGATGCAGAAAT+TGG	-	chr2_3:58582445-58582464	None:intergenic	30.0%
GACCTTGAAAGAAATAATCA+TGG	+	chr2_3:58582924-58582943	Msa0302030:intron	30.0%
ATCTCCATGATTATTGCTTT+CGG	+	chr2_3:58583196-58583215	Msa0302030:intron	30.0%
AGAGAATAACTATTGCTATG+TGG	-	chr2_3:58583624-58583643	None:intergenic	30.0%
TAGAATTCAATCAGTGGTAA+TGG	-	chr2_3:58583654-58583673	None:intergenic	30.0%
TAAGCTATTCTTGTGCAAAT+GGG	-	chr2_3:58584368-58584387	None:intergenic	30.0%
ATAAGCTATTCTTGTGCAAA+TGG	-	chr2_3:58584369-58584388	None:intergenic	30.0%
TATACATAAAAGGTCCTGTT+TGG	-	chr2_3:58584447-58584466	None:intergenic	30.0%
CATCACCTACAAAAAGAAAA+AGG	-	chr2_3:58584527-58584546	None:intergenic	30.0%
AGCTGACAAAAAGAAAATCA+AGG	+	chr2_3:58584827-58584846	Msa0302030:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	58582281	58585638	58582281	ID=Msa0302030;Name=Msa0302030
chr2_3	mRNA	58582281	58585638	58582281	ID=Msa0302030-mRNA-1;Parent=Msa0302030;Name=Msa0302030-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77\|1\|1\|1\|1\|1\|3\|486\|154
chr2_3	exon	58582281	58582373	58582281	ID=Msa0302030-mRNA-1:exon:18897;Parent=Msa0302030-mRNA-1
chr2_3	exon	58584541	58584904	58584541	ID=Msa0302030-mRNA-1:exon:18898;Parent=Msa0302030-mRNA-1
chr2_3	exon	58585068	58585638	58585068	ID=Msa0302030-mRNA-1:exon:18899;Parent=Msa0302030-mRNA-1
chr2_3	five_prime_UTR	58582281	58582357	58582281	ID=Msa0302030-mRNA-1:five_prime_utr;Parent=Msa0302030-mRNA-1
chr2_3	CDS	58582358	58582373	58582358	ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3	CDS	58584541	58584904	58584541	ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3	CDS	58585068	58585152	58585068	ID=Msa0302030-mRNA-1:cds;Parent=Msa0302030-mRNA-1
chr2_3	three_prime_UTR	58585153	58585638	58585153	ID=Msa0302030-mRNA-1:three_prime_utr;Parent=Msa0302030-mRNA-1

Gene Sequence

>Msa0302030

ATGGCTCCACCAAAAGGTGATGGTGCAAAGAAGACCGATCCAAAGGCACAGGCCTTGAAAGCTGCGAAAGCAGTGAAGTCAGGCACTGCCATCAAGAAGAAGGCAAAGAAGATCAGGACAACTGTTACATTCCACAGGCCAAAGACTTTATCTAGGGATAGGAATCCAAAATACCCTCGGATTAGTGCTACACCAAGGAACAAACTTGATCATTATCAGATTTTGAAGTATCCGCTCACAACTGAGTCAGCAATGAAGAAGATTGAAGACAACAACACTTTGGTTTTCATTGTTGACTTGCGAGCTGACAAAAAGAAAATCAAGGATGCCGTGAAGAAAATGTACGACATTCAGGCCAAGAAAGTTAACACCTTGATCAGGCCCGATGGCACCAAGAAGGCCTATGTAAGGTTGACACCTGATTATGATGCTTTGGATGTTGCAAACAAGATTGGGATTATTTAA

Protein sequence

>Msa0302030

MAPPKGDGAKKTDPKAQALKAAKAVKSGTAIKKKAKKIRTTVTFHRPKTLSRDRNPKYPRISATPRNKLDHYQILKYPLTTESAMKKIEDNNTLVFIVDLRADKKKIKDAVKKMYDIQAKKVNTLIRPDGTKKAYVRLTPDYDALDVANKIGII*