AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0302590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302590 MtrunA17_Chr2g0328821 100.000 180 0 0 34 213 15 194 5.25e-134 374
Msa0302590 MtrunA17_Chr5g0442931 100.000 178 0 0 36 213 1 178 7.64e-133 370
Msa0302590 MtrunA17_Chr5g0442901 100.000 178 0 0 36 213 1 178 7.64e-133 370
Msa0302590 MtrunA17_Chr3g0095911 94.382 178 10 0 36 213 1 178 6.12e-125 350
Msa0302590 MtrunA17_Chr2g0286131 62.353 85 32 0 127 211 1 85 6.44e-34 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0302590 AT2G34480.2 82.828 198 33 1 17 213 20 217 9.97e-121 341
Msa0302590 AT2G34480.1 90.230 174 17 0 40 213 5 178 1.05e-118 335
Msa0302590 AT1G29965.1 87.079 178 23 0 36 213 1 178 4.53e-117 331
Msa0302590 AT3G14600.1 87.079 178 23 0 36 213 1 178 1.33e-116 329
Msa0302590 AT1G29970.2 89.017 173 19 0 41 213 140 312 2.74e-114 328

Find 66 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCAATGGGAACTTGATTT+TGG 0.119520 2_3:+59407192 None:intergenic
GAAAAGTAATGGACAAGTTT+TGG 0.246012 2_3:-59408145 Msa0302590:CDS
AGATCAAGAACTATGGAATT+TGG 0.253330 2_3:-59407414 Msa0302590:CDS
GTTGGGCTTCTTAGCTTTGT+AGG 0.272211 2_3:+59407129 None:intergenic
ACGAAATGGCATCTCGTCAT+AGG 0.313310 2_3:-59407303 Msa0302590:CDS
ACTACAATCAGCTCGGAAAA+TGG 0.314844 2_3:-59408503 Msa0302590:CDS
AGATTTATCGCATGAAGCTT+TGG 0.333905 2_3:-59408320 Msa0302590:CDS
AAAAGGTCCACCATTGATTA+GGG 0.340282 2_3:-59408581 Msa0302590:CDS
GATTTATCGCATGAAGCTTT+GGG 0.349457 2_3:-59408319 Msa0302590:CDS
GTCAGTACTTTAAGAGCTTT+CGG 0.352974 2_3:-59407045 Msa0302590:three_prime_UTR
TGTTTGTGCACTCTTATTGC+TGG 0.354017 2_3:-59406821 Msa0302590:three_prime_UTR
AACTTACCAGAACTTGGATT+TGG 0.367421 2_3:+59408278 None:intergenic
CCTACCAAGATCAAGAACTA+TGG 0.389486 2_3:-59407421 Msa0302590:CDS
TCGAACTCAATGGGTCAAAA+AGG 0.390300 2_3:-59408598 None:intergenic
TCCCAGCTAATCTGTGCAAA+AGG 0.391912 2_3:-59407240 Msa0302590:CDS
AATTCCATAGTTCTTGATCT+TGG 0.400595 2_3:+59407417 None:intergenic
ATTCACTGAATCACATAAAC+AGG 0.404006 2_3:+59407107 None:intergenic
CATTGGTGTACAAGAAGATT+AGG 0.404454 2_3:-59407177 Msa0302590:CDS
AGCTTCATGCGATAAATCTT+AGG 0.407205 2_3:+59408324 None:intergenic
CTGTCTGAGTTTGCTGGTAA+TGG 0.414348 2_3:+59406998 None:intergenic
TGGTGGCGGTCTTAATGATC+TGG 0.424945 2_3:+59407263 None:intergenic
TACCGTGATACTACTCTAAA+CGG 0.448863 2_3:-59407346 Msa0302590:CDS
ATAAGGAACTTACCAGAACT+TGG 0.449276 2_3:+59408272 None:intergenic
ACAAAATACTTGATAAATAA+CGG 0.456124 2_3:+59406956 None:intergenic
TTCGTGCCAAATCCAAGTTC+TGG 0.468779 2_3:-59408284 Msa0302590:intron
CGAACACTGTCTGAGTTTGC+TGG 0.479837 2_3:+59406992 None:intergenic
TAAAATAAAACCCTAATCAA+TGG 0.482054 2_3:+59408571 None:intergenic
CTGAATCACATAAACAGGTT+GGG 0.486125 2_3:+59407112 None:intergenic
CTCTTCCGACAACCTGGTAC+TGG 0.487827 2_3:+59408373 None:intergenic
TTTCTTGAGGAAGTTGAAGA+AGG 0.490067 2_3:-59408172 Msa0302590:CDS
GGAGACAATGTATAACGAAA+TGG 0.490809 2_3:-59407317 Msa0302590:CDS
TAATCTTCTTGTACACCAAT+GGG 0.498182 2_3:+59407179 None:intergenic
TGGCATCTCGTCATAGGGTC+AGG 0.499078 2_3:-59407297 Msa0302590:CDS
TTGCGTTATCAGAGTCGTAC+TGG 0.505845 2_3:-59407391 Msa0302590:CDS
AGAAGATTAGGCCTCCTACT+AGG 0.511127 2_3:-59407165 Msa0302590:CDS
TCCACAGTTTCACCAGTACC+AGG 0.511898 2_3:-59408385 Msa0302590:intron
TTGATGTACCGGTACAAGCT+TGG 0.513924 2_3:-59406895 Msa0302590:three_prime_UTR
AAGAAGGTTAAGAAAAGTAA+TGG 0.518066 2_3:-59408156 Msa0302590:CDS
TTTGCACAGATTAGCTGGGA+TGG 0.520373 2_3:+59407243 None:intergenic
CCATAGTTCTTGATCTTGGT+AGG 0.522322 2_3:+59407421 None:intergenic
CAAGCAACTCTAACATGAGA+AGG 0.524095 2_3:+59407076 None:intergenic
CCCAGCTAATCTGTGCAAAA+GGG 0.524734 2_3:-59407239 Msa0302590:CDS
AAGAGCACTTCCCACCGAAA+AGG 0.530461 2_3:-59408355 Msa0302590:CDS
TTTCACCAGTACCAGGTTGT+CGG 0.538716 2_3:-59408378 Msa0302590:CDS
CTAATCTTCTTGTACACCAA+TGG 0.540589 2_3:+59407178 None:intergenic
CGAAATGGCATCTCGTCATA+GGG 0.544214 2_3:-59407302 Msa0302590:CDS
TCGGAAAATGGTTAACGCTA+AGG 0.552440 2_3:-59408491 Msa0302590:intron
GGATTTGGCACGAACCTCGT+TGG 0.555937 2_3:+59408293 None:intergenic
CAGATTAGCTGGGATGGTGG+CGG 0.559030 2_3:+59407249 None:intergenic
TTCCAAAATCAAGTTCCCAT+TGG 0.559786 2_3:-59407194 Msa0302590:CDS
ACTGAATCACATAAACAGGT+TGG 0.563649 2_3:+59407111 None:intergenic
ATGCTAATAGCTAATGAGCT+AGG 0.567864 2_3:-59406782 Msa0302590:three_prime_UTR
ATTCAAAACCAAGCTTGTAC+CGG 0.570756 2_3:+59406887 None:intergenic
GTCTTAATGATCTGGATGCA+AGG 0.582689 2_3:+59407271 None:intergenic
GAAGTGCTCTTCCGACAACC+TGG 0.588662 2_3:+59408367 None:intergenic
GCACAGATTAGCTGGGATGG+TGG 0.592241 2_3:+59407246 None:intergenic
AATAAAACCCTAATCAATGG+TGG 0.601555 2_3:+59408574 None:intergenic
AAGCAACTCTAACATGAGAA+GGG 0.603748 2_3:+59407077 None:intergenic
GCTGATGAACGAAGCTGTGT+TGG 0.604991 2_3:-59406751 Msa0302590:three_prime_UTR
TGGTTATCACAATATGTACA+AGG 0.613559 2_3:-59407371 Msa0302590:CDS
ACAGAAAACTACAATCAGCT+CGG 0.620129 2_3:-59408510 Msa0302590:CDS
TACTACTCTAAACGGTGCTG+TGG 0.650976 2_3:-59407338 Msa0302590:CDS
ATTGTTGTAGGTATTTCTTG+AGG 0.651477 2_3:-59408185 Msa0302590:intron
CACCGTTTAGAGTAGTATCA+CGG 0.660256 2_3:+59407344 None:intergenic
TACTTGATAAATAACGGACT+AGG 0.668215 2_3:+59406962 None:intergenic
GAAGCTTTGGGCAACCAACG+AGG 0.727612 2_3:-59408307 Msa0302590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAATGTTAATTGTTGT+AGG - chr2_3:59407138-59407157 Msa0302590:CDS 15.0%
!! ACAAAATACTTGATAAATAA+CGG + chr2_3:59408382-59408401 None:intergenic 15.0%
!! TTAATAATGTTAATTGTTGT+AGG - chr2_3:59407138-59407157 Msa0302590:CDS 15.0%
!! ACAAAATACTTGATAAATAA+CGG + chr2_3:59408382-59408401 None:intergenic 15.0%
!! TAAAATAAAACCCTAATCAA+TGG + chr2_3:59406767-59406786 None:intergenic 20.0%
!! TGTATCAAATTTGAATTAAG+AGG + chr2_3:59407249-59407268 None:intergenic 20.0%
!! ATTCAAATTTGATACAACTT+AGG - chr2_3:59407253-59407272 Msa0302590:CDS 20.0%
!! AAATCATTGAAATGTCATAT+AGG + chr2_3:59407370-59407389 None:intergenic 20.0%
!! TTGTTGTAACATATCTATTA+AGG - chr2_3:59407595-59407614 Msa0302590:intron 20.0%
!! TAAAATAAAACCCTAATCAA+TGG + chr2_3:59406767-59406786 None:intergenic 20.0%
!! TGTATCAAATTTGAATTAAG+AGG + chr2_3:59407249-59407268 None:intergenic 20.0%
!! ATTCAAATTTGATACAACTT+AGG - chr2_3:59407253-59407272 Msa0302590:CDS 20.0%
!! AAATCATTGAAATGTCATAT+AGG + chr2_3:59407370-59407389 None:intergenic 20.0%
!! TTGTTGTAACATATCTATTA+AGG - chr2_3:59407595-59407614 Msa0302590:intron 20.0%
! AAGAAGGTTAAGAAAAGTAA+TGG - chr2_3:59407179-59407198 Msa0302590:CDS 25.0%
! TTATGTCAAAATCCAAACAT+AGG - chr2_3:59407565-59407584 Msa0302590:intron 25.0%
! AATAGATATGTTACAACAAC+AGG + chr2_3:59407594-59407613 None:intergenic 25.0%
! CGTATTGTTTCAATTATTCT+AGG - chr2_3:59407747-59407766 Msa0302590:intron 25.0%
! CAATTATTCTAGGCAAAATA+AGG - chr2_3:59407757-59407776 Msa0302590:intron 25.0%
!! TTTTTCTCTTTTTGATGTAC+CGG - chr2_3:59408429-59408448 Msa0302590:intron 25.0%
!!! TAGTGAACTTTTTGTTTTGA+AGG - chr2_3:59408472-59408491 Msa0302590:intron 25.0%
! AAGAAGGTTAAGAAAAGTAA+TGG - chr2_3:59407179-59407198 Msa0302590:CDS 25.0%
! TTATGTCAAAATCCAAACAT+AGG - chr2_3:59407565-59407584 Msa0302590:intron 25.0%
! AATAGATATGTTACAACAAC+AGG + chr2_3:59407594-59407613 None:intergenic 25.0%
! CGTATTGTTTCAATTATTCT+AGG - chr2_3:59407747-59407766 Msa0302590:intron 25.0%
! CAATTATTCTAGGCAAAATA+AGG - chr2_3:59407757-59407776 Msa0302590:intron 25.0%
!! TTTTTCTCTTTTTGATGTAC+CGG - chr2_3:59408429-59408448 Msa0302590:intron 25.0%
!!! TAGTGAACTTTTTGTTTTGA+AGG - chr2_3:59408472-59408491 Msa0302590:intron 25.0%
AATAAAACCCTAATCAATGG+TGG + chr2_3:59406764-59406783 None:intergenic 30.0%
TTTGTGTGTGAATCTTAACA+CGG - chr2_3:59406912-59406931 Msa0302590:three_prime_UTR 30.0%
ATTGTTGTAGGTATTTCTTG+AGG - chr2_3:59407150-59407169 Msa0302590:CDS 30.0%
!!! GAAAAGTAATGGACAAGTTT+TGG - chr2_3:59407190-59407209 Msa0302590:CDS 30.0%
TCATAAACTGTATCATGGAA+TGG + chr2_3:59407409-59407428 None:intergenic 30.0%
ACAAGTCATAAACTGTATCA+TGG + chr2_3:59407414-59407433 None:intergenic 30.0%
!! TGACTTGTTTGAAAGTACTT+TGG - chr2_3:59407426-59407445 Msa0302590:CDS 30.0%
!! TTGTTTGAAAGTACTTTGGT+TGG - chr2_3:59407430-59407449 Msa0302590:CDS 30.0%
!! TGTTTGAAAGTACTTTGGTT+GGG - chr2_3:59407431-59407450 Msa0302590:CDS 30.0%
AATTGTGGTGAAAGAATATG+TGG - chr2_3:59407782-59407801 Msa0302590:intron 30.0%
TAACAACTAAAGCAAACACA+GGG + chr2_3:59407818-59407837 None:intergenic 30.0%
ATAACAACTAAAGCAAACAC+AGG + chr2_3:59407819-59407838 None:intergenic 30.0%
AATTCCATAGTTCTTGATCT+TGG + chr2_3:59407921-59407940 None:intergenic 30.0%
AGATCAAGAACTATGGAATT+TGG - chr2_3:59407921-59407940 Msa0302590:intron 30.0%
TGGTTATCACAATATGTACA+AGG - chr2_3:59407964-59407983 Msa0302590:intron 30.0%
TAATCTTCTTGTACACCAAT+GGG + chr2_3:59408159-59408178 None:intergenic 30.0%
!!! TAGCTTTGTAGGTTGTTTTT+AGG + chr2_3:59408198-59408217 None:intergenic 30.0%
ATTCACTGAATCACATAAAC+AGG + chr2_3:59408231-59408250 None:intergenic 30.0%
!! TACTTGATAAATAACGGACT+AGG + chr2_3:59408376-59408395 None:intergenic 30.0%
!!! TGAACTTTTTGTTTTGAAGG+AGG - chr2_3:59408475-59408494 Msa0302590:intron 30.0%
!!! GAACTTTTTGTTTTGAAGGA+GGG - chr2_3:59408476-59408495 Msa0302590:intron 30.0%
AATAAAACCCTAATCAATGG+TGG + chr2_3:59406764-59406783 None:intergenic 30.0%
TTTGTGTGTGAATCTTAACA+CGG - chr2_3:59406912-59406931 Msa0302590:three_prime_UTR 30.0%
ATTGTTGTAGGTATTTCTTG+AGG - chr2_3:59407150-59407169 Msa0302590:CDS 30.0%
!!! GAAAAGTAATGGACAAGTTT+TGG - chr2_3:59407190-59407209 Msa0302590:CDS 30.0%
TCATAAACTGTATCATGGAA+TGG + chr2_3:59407409-59407428 None:intergenic 30.0%
ACAAGTCATAAACTGTATCA+TGG + chr2_3:59407414-59407433 None:intergenic 30.0%
!! TGACTTGTTTGAAAGTACTT+TGG - chr2_3:59407426-59407445 Msa0302590:CDS 30.0%
!! TTGTTTGAAAGTACTTTGGT+TGG - chr2_3:59407430-59407449 Msa0302590:CDS 30.0%
!! TGTTTGAAAGTACTTTGGTT+GGG - chr2_3:59407431-59407450 Msa0302590:CDS 30.0%
AATTGTGGTGAAAGAATATG+TGG - chr2_3:59407782-59407801 Msa0302590:intron 30.0%
TAACAACTAAAGCAAACACA+GGG + chr2_3:59407818-59407837 None:intergenic 30.0%
ATAACAACTAAAGCAAACAC+AGG + chr2_3:59407819-59407838 None:intergenic 30.0%
AATTCCATAGTTCTTGATCT+TGG + chr2_3:59407921-59407940 None:intergenic 30.0%
AGATCAAGAACTATGGAATT+TGG - chr2_3:59407921-59407940 Msa0302590:intron 30.0%
TGGTTATCACAATATGTACA+AGG - chr2_3:59407964-59407983 Msa0302590:intron 30.0%
TAATCTTCTTGTACACCAAT+GGG + chr2_3:59408159-59408178 None:intergenic 30.0%
!!! TAGCTTTGTAGGTTGTTTTT+AGG + chr2_3:59408198-59408217 None:intergenic 30.0%
ATTCACTGAATCACATAAAC+AGG + chr2_3:59408231-59408250 None:intergenic 30.0%
!! TACTTGATAAATAACGGACT+AGG + chr2_3:59408376-59408395 None:intergenic 30.0%
!!! TGAACTTTTTGTTTTGAAGG+AGG - chr2_3:59408475-59408494 Msa0302590:intron 30.0%
!!! GAACTTTTTGTTTTGAAGGA+GGG - chr2_3:59408476-59408495 Msa0302590:intron 30.0%
! AAAAAGGTCCACCATTGATT+AGG - chr2_3:59406753-59406772 Msa0302590:three_prime_UTR 35.0%
! AAAAGGTCCACCATTGATTA+GGG - chr2_3:59406754-59406773 Msa0302590:three_prime_UTR 35.0%
ACAGAAAACTACAATCAGCT+CGG - chr2_3:59406825-59406844 Msa0302590:three_prime_UTR 35.0%
! CTTAGGATGTTCATCCTTTT+CGG + chr2_3:59406997-59407016 None:intergenic 35.0%
AGCTTCATGCGATAAATCTT+AGG + chr2_3:59407014-59407033 None:intergenic 35.0%
AGATTTATCGCATGAAGCTT+TGG - chr2_3:59407015-59407034 Msa0302590:three_prime_UTR 35.0%
GATTTATCGCATGAAGCTTT+GGG - chr2_3:59407016-59407035 Msa0302590:three_prime_UTR 35.0%
AACTTACCAGAACTTGGATT+TGG + chr2_3:59407060-59407079 None:intergenic 35.0%
ATAAGGAACTTACCAGAACT+TGG + chr2_3:59407066-59407085 None:intergenic 35.0%
TTTCTTGAGGAAGTTGAAGA+AGG - chr2_3:59407163-59407182 Msa0302590:CDS 35.0%
!!! ACAAGTTTTGGCTATCAATG+AGG - chr2_3:59407202-59407221 Msa0302590:CDS 35.0%
! AAAGTACTTTGGTTGGGAAA+GGG - chr2_3:59407437-59407456 Msa0302590:CDS 35.0%
!!! TGTTCTGTTTTGATTAGTGC+TGG - chr2_3:59407462-59407481 Msa0302590:intron 35.0%
!!! GTTCTGTTTTGATTAGTGCT+GGG - chr2_3:59407463-59407482 Msa0302590:intron 35.0%
ACAGCTTACACTCAAATGTT+TGG + chr2_3:59407529-59407548 None:intergenic 35.0%
AACAACAGGTTACCTATGTT+TGG + chr2_3:59407580-59407599 None:intergenic 35.0%
TGTCTTGAGTATTTCCTAGA+GGG - chr2_3:59407658-59407677 Msa0302590:intron 35.0%
TCAGCTATGAAATTAGTCAC+TGG + chr2_3:59407697-59407716 None:intergenic 35.0%
AGGCAAAATAAGGCTAATTG+TGG - chr2_3:59407767-59407786 Msa0302590:intron 35.0%
! ACCTTCAATTGCAGTCTTTT+TGG - chr2_3:59407842-59407861 Msa0302590:intron 35.0%
TCCAAAAAGACTGCAATTGA+AGG + chr2_3:59407846-59407865 None:intergenic 35.0%
! ATTGCAGTCTTTTTGGACAT+AGG - chr2_3:59407849-59407868 Msa0302590:intron 35.0%
! TTGCAGTCTTTTTGGACATA+GGG - chr2_3:59407850-59407869 Msa0302590:intron 35.0%
! TTTTTGGACATAGGGGTTTA+TGG - chr2_3:59407858-59407877 Msa0302590:intron 35.0%
TACCGTGATACTACTCTAAA+CGG - chr2_3:59407989-59408008 Msa0302590:intron 35.0%
GGAGACAATGTATAACGAAA+TGG - chr2_3:59408018-59408037 Msa0302590:intron 35.0%
!!! GGAACTTGATTTTGGAATTG+TGG + chr2_3:59408138-59408157 None:intergenic 35.0%
TTCCAAAATCAAGTTCCCAT+TGG - chr2_3:59408141-59408160 Msa0302590:CDS 35.0%
CTAATCTTCTTGTACACCAA+TGG + chr2_3:59408160-59408179 None:intergenic 35.0%
CATTGGTGTACAAGAAGATT+AGG - chr2_3:59408158-59408177 Msa0302590:CDS 35.0%
CTGAATCACATAAACAGGTT+GGG + chr2_3:59408226-59408245 None:intergenic 35.0%
ACTGAATCACATAAACAGGT+TGG + chr2_3:59408227-59408246 None:intergenic 35.0%
AAGCAACTCTAACATGAGAA+GGG + chr2_3:59408261-59408280 None:intergenic 35.0%
! GTCAGTACTTTAAGAGCTTT+CGG - chr2_3:59408290-59408309 Msa0302590:CDS 35.0%
ATTCAAAACCAAGCTTGTAC+CGG + chr2_3:59408451-59408470 None:intergenic 35.0%
ATGCTAATAGCTAATGAGCT+AGG - chr2_3:59408553-59408572 Msa0302590:CDS 35.0%
! AAAAAGGTCCACCATTGATT+AGG - chr2_3:59406753-59406772 Msa0302590:three_prime_UTR 35.0%
! AAAAGGTCCACCATTGATTA+GGG - chr2_3:59406754-59406773 Msa0302590:three_prime_UTR 35.0%
ACAGAAAACTACAATCAGCT+CGG - chr2_3:59406825-59406844 Msa0302590:three_prime_UTR 35.0%
! CTTAGGATGTTCATCCTTTT+CGG + chr2_3:59406997-59407016 None:intergenic 35.0%
AGCTTCATGCGATAAATCTT+AGG + chr2_3:59407014-59407033 None:intergenic 35.0%
AGATTTATCGCATGAAGCTT+TGG - chr2_3:59407015-59407034 Msa0302590:three_prime_UTR 35.0%
GATTTATCGCATGAAGCTTT+GGG - chr2_3:59407016-59407035 Msa0302590:three_prime_UTR 35.0%
AACTTACCAGAACTTGGATT+TGG + chr2_3:59407060-59407079 None:intergenic 35.0%
ATAAGGAACTTACCAGAACT+TGG + chr2_3:59407066-59407085 None:intergenic 35.0%
TTTCTTGAGGAAGTTGAAGA+AGG - chr2_3:59407163-59407182 Msa0302590:CDS 35.0%
!!! ACAAGTTTTGGCTATCAATG+AGG - chr2_3:59407202-59407221 Msa0302590:CDS 35.0%
! AAAGTACTTTGGTTGGGAAA+GGG - chr2_3:59407437-59407456 Msa0302590:CDS 35.0%
!!! TGTTCTGTTTTGATTAGTGC+TGG - chr2_3:59407462-59407481 Msa0302590:intron 35.0%
!!! GTTCTGTTTTGATTAGTGCT+GGG - chr2_3:59407463-59407482 Msa0302590:intron 35.0%
ACAGCTTACACTCAAATGTT+TGG + chr2_3:59407529-59407548 None:intergenic 35.0%
AACAACAGGTTACCTATGTT+TGG + chr2_3:59407580-59407599 None:intergenic 35.0%
TGTCTTGAGTATTTCCTAGA+GGG - chr2_3:59407658-59407677 Msa0302590:intron 35.0%
TCAGCTATGAAATTAGTCAC+TGG + chr2_3:59407697-59407716 None:intergenic 35.0%
AGGCAAAATAAGGCTAATTG+TGG - chr2_3:59407767-59407786 Msa0302590:intron 35.0%
! ACCTTCAATTGCAGTCTTTT+TGG - chr2_3:59407842-59407861 Msa0302590:intron 35.0%
TCCAAAAAGACTGCAATTGA+AGG + chr2_3:59407846-59407865 None:intergenic 35.0%
! ATTGCAGTCTTTTTGGACAT+AGG - chr2_3:59407849-59407868 Msa0302590:intron 35.0%
! TTGCAGTCTTTTTGGACATA+GGG - chr2_3:59407850-59407869 Msa0302590:intron 35.0%
! TTTTTGGACATAGGGGTTTA+TGG - chr2_3:59407858-59407877 Msa0302590:intron 35.0%
TACCGTGATACTACTCTAAA+CGG - chr2_3:59407989-59408008 Msa0302590:intron 35.0%
GGAGACAATGTATAACGAAA+TGG - chr2_3:59408018-59408037 Msa0302590:intron 35.0%
!!! GGAACTTGATTTTGGAATTG+TGG + chr2_3:59408138-59408157 None:intergenic 35.0%
TTCCAAAATCAAGTTCCCAT+TGG - chr2_3:59408141-59408160 Msa0302590:CDS 35.0%
CTAATCTTCTTGTACACCAA+TGG + chr2_3:59408160-59408179 None:intergenic 35.0%
CATTGGTGTACAAGAAGATT+AGG - chr2_3:59408158-59408177 Msa0302590:CDS 35.0%
CTGAATCACATAAACAGGTT+GGG + chr2_3:59408226-59408245 None:intergenic 35.0%
ACTGAATCACATAAACAGGT+TGG + chr2_3:59408227-59408246 None:intergenic 35.0%
AAGCAACTCTAACATGAGAA+GGG + chr2_3:59408261-59408280 None:intergenic 35.0%
! GTCAGTACTTTAAGAGCTTT+CGG - chr2_3:59408290-59408309 Msa0302590:CDS 35.0%
ATTCAAAACCAAGCTTGTAC+CGG + chr2_3:59408451-59408470 None:intergenic 35.0%
ATGCTAATAGCTAATGAGCT+AGG - chr2_3:59408553-59408572 Msa0302590:CDS 35.0%
ACTACAATCAGCTCGGAAAA+TGG - chr2_3:59406832-59406851 Msa0302590:three_prime_UTR 40.0%
TCGGAAAATGGTTAACGCTA+AGG - chr2_3:59406844-59406863 Msa0302590:three_prime_UTR 40.0%
GATACAACTTAGGCTGTGTT+TGG - chr2_3:59407263-59407282 Msa0302590:CDS 40.0%
ATACCGAACAAGTGCTTCTA+GGG + chr2_3:59407325-59407344 None:intergenic 40.0%
AATACCGAACAAGTGCTTCT+AGG + chr2_3:59407326-59407345 None:intergenic 40.0%
!! GAAAGTACTTTGGTTGGGAA+AGG - chr2_3:59407436-59407455 Msa0302590:CDS 40.0%
! CTCTAGGAAATACTCAAGAC+AGG + chr2_3:59407659-59407678 None:intergenic 40.0%
CTGTCTTGAGTATTTCCTAG+AGG - chr2_3:59407657-59407676 Msa0302590:intron 40.0%
GTTTCATGCTAAAACCCTCT+AGG + chr2_3:59407675-59407694 None:intergenic 40.0%
GGTGAAAGAATATGTGGAGT+AGG - chr2_3:59407788-59407807 Msa0302590:intron 40.0%
! TGCAGTCTTTTTGGACATAG+GGG - chr2_3:59407851-59407870 Msa0302590:intron 40.0%
CCATAGTTCTTGATCTTGGT+AGG + chr2_3:59407917-59407936 None:intergenic 40.0%
CCTACCAAGATCAAGAACTA+TGG - chr2_3:59407914-59407933 Msa0302590:intron 40.0%
! CACCGTTTAGAGTAGTATCA+CGG + chr2_3:59407994-59408013 None:intergenic 40.0%
GTCTTAATGATCTGGATGCA+AGG + chr2_3:59408067-59408086 None:intergenic 40.0%
! CACCAATGGGAACTTGATTT+TGG + chr2_3:59408146-59408165 None:intergenic 40.0%
!!! GTTGTTTTTAGGCTCCTAGT+AGG + chr2_3:59408187-59408206 None:intergenic 40.0%
CAAGCAACTCTAACATGAGA+AGG + chr2_3:59408262-59408281 None:intergenic 40.0%
TGTTTGTGCACTCTTATTGC+TGG - chr2_3:59408514-59408533 Msa0302590:CDS 40.0%
ACTACAATCAGCTCGGAAAA+TGG - chr2_3:59406832-59406851 Msa0302590:three_prime_UTR 40.0%
TCGGAAAATGGTTAACGCTA+AGG - chr2_3:59406844-59406863 Msa0302590:three_prime_UTR 40.0%
GATACAACTTAGGCTGTGTT+TGG - chr2_3:59407263-59407282 Msa0302590:CDS 40.0%
ATACCGAACAAGTGCTTCTA+GGG + chr2_3:59407325-59407344 None:intergenic 40.0%
AATACCGAACAAGTGCTTCT+AGG + chr2_3:59407326-59407345 None:intergenic 40.0%
!! GAAAGTACTTTGGTTGGGAA+AGG - chr2_3:59407436-59407455 Msa0302590:CDS 40.0%
! CTCTAGGAAATACTCAAGAC+AGG + chr2_3:59407659-59407678 None:intergenic 40.0%
CTGTCTTGAGTATTTCCTAG+AGG - chr2_3:59407657-59407676 Msa0302590:intron 40.0%
GTTTCATGCTAAAACCCTCT+AGG + chr2_3:59407675-59407694 None:intergenic 40.0%
GGTGAAAGAATATGTGGAGT+AGG - chr2_3:59407788-59407807 Msa0302590:intron 40.0%
! TGCAGTCTTTTTGGACATAG+GGG - chr2_3:59407851-59407870 Msa0302590:intron 40.0%
CCATAGTTCTTGATCTTGGT+AGG + chr2_3:59407917-59407936 None:intergenic 40.0%
CCTACCAAGATCAAGAACTA+TGG - chr2_3:59407914-59407933 Msa0302590:intron 40.0%
! CACCGTTTAGAGTAGTATCA+CGG + chr2_3:59407994-59408013 None:intergenic 40.0%
GTCTTAATGATCTGGATGCA+AGG + chr2_3:59408067-59408086 None:intergenic 40.0%
! CACCAATGGGAACTTGATTT+TGG + chr2_3:59408146-59408165 None:intergenic 40.0%
!!! GTTGTTTTTAGGCTCCTAGT+AGG + chr2_3:59408187-59408206 None:intergenic 40.0%
CAAGCAACTCTAACATGAGA+AGG + chr2_3:59408262-59408281 None:intergenic 40.0%
TGTTTGTGCACTCTTATTGC+TGG - chr2_3:59408514-59408533 Msa0302590:CDS 40.0%
TTTCACCAGTACCAGGTTGT+CGG - chr2_3:59406957-59406976 Msa0302590:three_prime_UTR 45.0%
! GGATGTTCATCCTTTTCGGT+GGG + chr2_3:59406993-59407012 None:intergenic 45.0%
! AGGATGTTCATCCTTTTCGG+TGG + chr2_3:59406994-59407013 None:intergenic 45.0%
TTCGTGCCAAATCCAAGTTC+TGG - chr2_3:59407051-59407070 Msa0302590:three_prime_UTR 45.0%
TACCGAACAAGTGCTTCTAG+GGG + chr2_3:59407324-59407343 None:intergenic 45.0%
GGCAAACAGTGACAGTTTGA+AGG + chr2_3:59407638-59407657 None:intergenic 45.0%
TTGCGTTATCAGAGTCGTAC+TGG - chr2_3:59407944-59407963 Msa0302590:intron 45.0%
! TACTACTCTAAACGGTGCTG+TGG - chr2_3:59407997-59408016 Msa0302590:intron 45.0%
ACGAAATGGCATCTCGTCAT+AGG - chr2_3:59408032-59408051 Msa0302590:intron 45.0%
CGAAATGGCATCTCGTCATA+GGG - chr2_3:59408033-59408052 Msa0302590:intron 45.0%
! TTTGCACAGATTAGCTGGGA+TGG + chr2_3:59408095-59408114 None:intergenic 45.0%
TCCCAGCTAATCTGTGCAAA+AGG - chr2_3:59408095-59408114 Msa0302590:intron 45.0%
!! CCCTTTTGCACAGATTAGCT+GGG + chr2_3:59408099-59408118 None:intergenic 45.0%
CCCAGCTAATCTGTGCAAAA+GGG - chr2_3:59408096-59408115 Msa0302590:intron 45.0%
! TCCCTTTTGCACAGATTAGC+TGG + chr2_3:59408100-59408119 None:intergenic 45.0%
AGAAGATTAGGCCTCCTACT+AGG - chr2_3:59408170-59408189 Msa0302590:CDS 45.0%
!! GTTTTTAGGCTCCTAGTAGG+AGG + chr2_3:59408184-59408203 None:intergenic 45.0%
!! GTTGGGCTTCTTAGCTTTGT+AGG + chr2_3:59408209-59408228 None:intergenic 45.0%
!! CTGTCTGAGTTTGCTGGTAA+TGG + chr2_3:59408340-59408359 None:intergenic 45.0%
! TTGATGTACCGGTACAAGCT+TGG - chr2_3:59408440-59408459 Msa0302590:intron 45.0%
TTTCACCAGTACCAGGTTGT+CGG - chr2_3:59406957-59406976 Msa0302590:three_prime_UTR 45.0%
! GGATGTTCATCCTTTTCGGT+GGG + chr2_3:59406993-59407012 None:intergenic 45.0%
! AGGATGTTCATCCTTTTCGG+TGG + chr2_3:59406994-59407013 None:intergenic 45.0%
TTCGTGCCAAATCCAAGTTC+TGG - chr2_3:59407051-59407070 Msa0302590:three_prime_UTR 45.0%
TACCGAACAAGTGCTTCTAG+GGG + chr2_3:59407324-59407343 None:intergenic 45.0%
GGCAAACAGTGACAGTTTGA+AGG + chr2_3:59407638-59407657 None:intergenic 45.0%
TTGCGTTATCAGAGTCGTAC+TGG - chr2_3:59407944-59407963 Msa0302590:intron 45.0%
! TACTACTCTAAACGGTGCTG+TGG - chr2_3:59407997-59408016 Msa0302590:intron 45.0%
ACGAAATGGCATCTCGTCAT+AGG - chr2_3:59408032-59408051 Msa0302590:intron 45.0%
CGAAATGGCATCTCGTCATA+GGG - chr2_3:59408033-59408052 Msa0302590:intron 45.0%
! TTTGCACAGATTAGCTGGGA+TGG + chr2_3:59408095-59408114 None:intergenic 45.0%
TCCCAGCTAATCTGTGCAAA+AGG - chr2_3:59408095-59408114 Msa0302590:intron 45.0%
!! CCCTTTTGCACAGATTAGCT+GGG + chr2_3:59408099-59408118 None:intergenic 45.0%
CCCAGCTAATCTGTGCAAAA+GGG - chr2_3:59408096-59408115 Msa0302590:intron 45.0%
! TCCCTTTTGCACAGATTAGC+TGG + chr2_3:59408100-59408119 None:intergenic 45.0%
AGAAGATTAGGCCTCCTACT+AGG - chr2_3:59408170-59408189 Msa0302590:CDS 45.0%
!! GTTTTTAGGCTCCTAGTAGG+AGG + chr2_3:59408184-59408203 None:intergenic 45.0%
!! GTTGGGCTTCTTAGCTTTGT+AGG + chr2_3:59408209-59408228 None:intergenic 45.0%
!! CTGTCTGAGTTTGCTGGTAA+TGG + chr2_3:59408340-59408359 None:intergenic 45.0%
! TTGATGTACCGGTACAAGCT+TGG - chr2_3:59408440-59408459 Msa0302590:intron 45.0%
!! TGTTAATTAATAAATAAATA+AGG + chr2_3:59407083-59407102 None:intergenic 5.0%
!! TGTTAATTAATAAATAAATA+AGG + chr2_3:59407083-59407102 None:intergenic 5.0%
TCCACAGTTTCACCAGTACC+AGG - chr2_3:59406950-59406969 Msa0302590:three_prime_UTR 50.0%
!! ACCTGGTACTGGTGAAACTG+TGG + chr2_3:59406954-59406973 None:intergenic 50.0%
!! AAGAGCACTTCCCACCGAAA+AGG - chr2_3:59406980-59406999 Msa0302590:three_prime_UTR 50.0%
!! GACCCCTAGAAGCACTTGTT+CGG - chr2_3:59407319-59407338 Msa0302590:CDS 50.0%
!! TGGTGGCGGTCTTAATGATC+TGG + chr2_3:59408075-59408094 None:intergenic 50.0%
CGAACACTGTCTGAGTTTGC+TGG + chr2_3:59408346-59408365 None:intergenic 50.0%
GCTGATGAACGAAGCTGTGT+TGG - chr2_3:59408584-59408603 Msa0302590:CDS 50.0%
TCCACAGTTTCACCAGTACC+AGG - chr2_3:59406950-59406969 Msa0302590:three_prime_UTR 50.0%
!! ACCTGGTACTGGTGAAACTG+TGG + chr2_3:59406954-59406973 None:intergenic 50.0%
!! AAGAGCACTTCCCACCGAAA+AGG - chr2_3:59406980-59406999 Msa0302590:three_prime_UTR 50.0%
!! GACCCCTAGAAGCACTTGTT+CGG - chr2_3:59407319-59407338 Msa0302590:CDS 50.0%
!! TGGTGGCGGTCTTAATGATC+TGG + chr2_3:59408075-59408094 None:intergenic 50.0%
CGAACACTGTCTGAGTTTGC+TGG + chr2_3:59408346-59408365 None:intergenic 50.0%
GCTGATGAACGAAGCTGTGT+TGG - chr2_3:59408584-59408603 Msa0302590:CDS 50.0%
CTCTTCCGACAACCTGGTAC+TGG + chr2_3:59406965-59406984 None:intergenic 55.0%
! GAAGTGCTCTTCCGACAACC+TGG + chr2_3:59406971-59406990 None:intergenic 55.0%
!! GAAGCTTTGGGCAACCAACG+AGG - chr2_3:59407028-59407047 Msa0302590:three_prime_UTR 55.0%
GGATTTGGCACGAACCTCGT+TGG + chr2_3:59407045-59407064 None:intergenic 55.0%
TGGCATCTCGTCATAGGGTC+AGG - chr2_3:59408038-59408057 Msa0302590:intron 55.0%
! CAGATTAGCTGGGATGGTGG+CGG + chr2_3:59408089-59408108 None:intergenic 55.0%
! GCACAGATTAGCTGGGATGG+TGG + chr2_3:59408092-59408111 None:intergenic 55.0%
CTCTTCCGACAACCTGGTAC+TGG + chr2_3:59406965-59406984 None:intergenic 55.0%
! GAAGTGCTCTTCCGACAACC+TGG + chr2_3:59406971-59406990 None:intergenic 55.0%
!! GAAGCTTTGGGCAACCAACG+AGG - chr2_3:59407028-59407047 Msa0302590:three_prime_UTR 55.0%
GGATTTGGCACGAACCTCGT+TGG + chr2_3:59407045-59407064 None:intergenic 55.0%
TGGCATCTCGTCATAGGGTC+AGG - chr2_3:59408038-59408057 Msa0302590:intron 55.0%
! CAGATTAGCTGGGATGGTGG+CGG + chr2_3:59408089-59408108 None:intergenic 55.0%
! GCACAGATTAGCTGGGATGG+TGG + chr2_3:59408092-59408111 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 59406746 59408611 59406746 ID=Msa0302590;Name=Msa0302590
chr2_3 mRNA 59406746 59408611 59406746 ID=Msa0302590-mRNA-1;Parent=Msa0302590;Name=Msa0302590-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.75|1|1|1|4|371|213
chr2_3 exon 59406746 59407458 59406746 ID=Msa0302590-mRNA-1:exon:19322;Parent=Msa0302590-mRNA-1
chr2_3 exon 59408134 59408197 59408134 ID=Msa0302590-mRNA-1:exon:19321;Parent=Msa0302590-mRNA-1
chr2_3 exon 59408285 59408400 59408285 ID=Msa0302590-mRNA-1:exon:19320;Parent=Msa0302590-mRNA-1
chr2_3 exon 59408492 59408611 59408492 ID=Msa0302590-mRNA-1:exon:19319;Parent=Msa0302590-mRNA-1
chr2_3 CDS 59408492 59408611 59408492 ID=Msa0302590-mRNA-1:cds;Parent=Msa0302590-mRNA-1
chr2_3 CDS 59408285 59408400 59408285 ID=Msa0302590-mRNA-1:cds;Parent=Msa0302590-mRNA-1
chr2_3 CDS 59408134 59408197 59408134 ID=Msa0302590-mRNA-1:cds;Parent=Msa0302590-mRNA-1
chr2_3 CDS 59407117 59407458 59407117 ID=Msa0302590-mRNA-1:cds;Parent=Msa0302590-mRNA-1
chr2_3 three_prime_UTR 59406746 59407116 59406746 ID=Msa0302590-mRNA-1:three_prime_utr;Parent=Msa0302590-mRNA-1
Gene Sequence

>Msa0302590

ATGGGTCAAAAAGGTCCACCATTGATTAGGGTTTTATTTTACATTGTAAATGCTGCTGCATTGATCTTCATTCACATTTACAGAAAACTACAATCAGCTCGGAAAATGGTTAACGCTAAGTTTCACCAGTACCAGGTTGTCGGAAGAGCACTTCCCACCGAAAAGGATGAACATCCTAAGATTTATCGCATGAAGCTTTGGGCAACCAACGAGGTTCGTGCCAAATCCAAGTTCTGGTATTTCTTGAGGAAGTTGAAGAAGGTTAAGAAAAGTAATGGACAAGTTTTGGCTATCAATGAGATCTTTGAGAAAAATCCTACCAAGATCAAGAACTATGGAATTTGGTTGCGTTATCAGAGTCGTACTGGTTATCACAATATGTACAAGGAATACCGTGATACTACTCTAAACGGTGCTGTGGAGACAATGTATAACGAAATGGCATCTCGTCATAGGGTCAGGTTTCCTTGCATCCAGATCATTAAGACCGCCACCATCCCAGCTAATCTGTGCAAAAGGGAGAGCACTAAGCAGTTCCACAATTCCAAAATCAAGTTCCCATTGGTGTACAAGAAGATTAGGCCTCCTACTAGGAGCCTAAAAACAACCTACAAAGCTAAGAAGCCCAACCTGTTTATGTGA
Protein sequence

>Msa0302590

MGQKGPPLIRVLFYIVNAAALIFIHIYRKLQSARKMVNAKFHQYQVVGRALPTEKDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTKIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVETMYNEMASRHRVRFPCIQIIKTATIPANLCKRESTKQFHNSKIKFPLVYKKIRPPTRSLKTTYKAKKPNLFM*