AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0303240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0303240 MtrunA17_Chr2g0329791 98.582 282 4 0 1 282 1 282 0.0 579
Msa0303240 MtrunA17_Chr2g0329781 81.890 254 43 2 32 282 10 263 2.95e-158 441
Msa0303240 MtrunA17_Chr3g0145981 35.759 316 149 8 9 282 22 325 3.01e-59 192
Msa0303240 MtrunA17_Chr8g0370851 36.957 322 151 9 4 282 1 313 4.15e-58 188
Msa0303240 MtrunA17_Chr8g0370821 37.584 298 137 9 30 282 31 324 4.72e-54 178
Msa0303240 MtrunA17_Chr7g0270841 39.241 237 108 6 81 282 36 271 6.25e-54 176
Msa0303240 MtrunA17_Chr8g0370831 37.282 287 140 9 31 282 21 302 2.05e-47 160
Msa0303240 MtrunA17_Chr2g0329771 80.000 80 16 0 1 80 1 80 1.01e-42 141
Msa0303240 MtrunA17_Chr2g0294721 31.120 241 121 8 86 282 62 301 2.73e-24 99.8
Msa0303240 MtrunA17_Chr2g0329761 81.132 53 10 0 229 281 3 55 3.33e-24 92.4
Msa0303240 MtrunA17_Chr1g0206111 27.273 253 131 7 78 282 60 307 9.69e-21 90.1
Msa0303240 MtrunA17_Chr7g0221751 25.714 315 171 12 14 282 7 304 2.48e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0303240 AT3G54420.1 66.312 282 85 3 1 282 2 273 7.71e-137 387
Msa0303240 AT2G43590.1 56.863 255 100 3 28 282 20 264 2.27e-106 310
Msa0303240 AT2G43580.1 52.941 255 111 3 28 282 20 265 5.75e-97 286
Msa0303240 AT2G43570.1 50.352 284 120 8 6 282 8 277 2.21e-89 267
Msa0303240 AT2G43620.1 45.683 278 130 8 18 282 14 283 4.33e-83 251
Msa0303240 AT2G43610.1 44.718 284 138 7 10 282 6 281 9.81e-83 250
Msa0303240 AT3G47540.1 45.982 224 98 5 60 282 13 214 3.74e-62 196
Msa0303240 AT1G56680.1 39.068 279 150 11 15 282 11 280 1.13e-59 191
Msa0303240 AT1G56680.2 39.068 279 150 11 15 282 34 303 2.58e-59 191
Msa0303240 AT3G47540.2 44.796 221 99 5 60 279 13 211 1.21e-57 186
Msa0303240 AT3G12500.1 39.286 280 132 6 37 282 45 320 1.89e-55 182
Msa0303240 AT2G43600.1 35.563 284 160 11 11 282 1 273 5.30e-54 176
Msa0303240 AT1G02360.1 39.916 238 107 5 80 282 29 265 1.21e-53 176
Msa0303240 AT2G43600.2 35.439 285 161 11 10 282 21 294 1.42e-53 176
Msa0303240 AT1G02360.3 39.916 238 107 5 80 282 81 317 2.98e-53 176
Msa0303240 AT1G02360.2 39.916 238 107 5 80 282 75 311 3.29e-53 176
Msa0303240 AT4G01700.1 38.912 239 110 5 79 282 36 273 4.27e-50 167
Msa0303240 AT1G05850.1 22.500 320 175 10 11 282 10 304 1.16e-20 90.1
Msa0303240 AT1G05850.2 22.500 320 175 10 11 282 10 304 1.16e-20 90.1
Msa0303240 AT3G16920.2 27.530 247 133 9 81 282 92 337 1.64e-19 87.4
Msa0303240 AT3G16920.1 27.530 247 133 9 81 282 68 313 1.66e-19 87.0

Find 85 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCATTGGCCACTGTTTC+AGG 0.200039 2_3:+60379186 None:intergenic
TTATTCAAATATATACTAAA+TGG 0.212718 2_3:+60378937 None:intergenic
GTTTGGTAGGTCTGGTTCTT+TGG 0.220014 2_3:-60379642 Msa0303240:CDS
GACAAATTGGTTACAAGATA+TGG 0.258298 2_3:-60378744 Msa0303240:three_prime_UTR
CGACCGTAGTAACCTTTGTT+AGG 0.286313 2_3:+60379285 None:intergenic
GAGGCACACGTTTGACAAAT+TGG 0.299206 2_3:-60378757 Msa0303240:three_prime_UTR
CTATCTTGGAACTACAACTA+TGG 0.300245 2_3:-60379249 Msa0303240:CDS
TCATCACAGTAGTCCTTTGA+TGG 0.329355 2_3:+60379333 None:intergenic
AAGGCTTTGGTGCTACCATT+AGG 0.339170 2_3:-60379101 Msa0303240:CDS
CTCAATTCTTATAATCAGTT+TGG 0.343244 2_3:-60379659 Msa0303240:CDS
TATTCAAATATATACTAAAT+GGG 0.351066 2_3:+60378938 None:intergenic
TATAATCAGTTTGGTAGGTC+TGG 0.352546 2_3:-60379650 Msa0303240:CDS
TCAAGACAGCATTATGGTAT+TGG 0.355700 2_3:-60379152 Msa0303240:CDS
GCTCATTTCACACATGAAAC+TGG 0.365058 2_3:-60379587 Msa0303240:CDS
GCTACCATTAGGGCTATTAA+TGG 0.378451 2_3:-60379090 Msa0303240:CDS
AGGCTTTGGTGCTACCATTA+GGG 0.385021 2_3:-60379100 Msa0303240:CDS
TGTCCTTCAAGACAGCATTA+TGG 0.387076 2_3:-60379158 Msa0303240:CDS
TGCTATACCGAAGAGATTGA+TGG 0.389108 2_3:-60379357 Msa0303240:CDS
AGCAGCTGCAACCTCACGTT+TGG 0.392131 2_3:+60379613 None:intergenic
ATACCATAATGCTGTCTTGA+AGG 0.394568 2_3:+60379155 None:intergenic
TAGGCAAACTTGGTGGTGTT+TGG 0.406151 2_3:+60379801 None:intergenic
TATATACTAAATGGGGTGTA+TGG 0.414478 2_3:+60378946 None:intergenic
GCCACATTAGCATCATTGTT+AGG 0.421338 2_3:+60379782 None:intergenic
GACCGTAGTAACCTTTGTTA+GGG 0.424083 2_3:+60379286 None:intergenic
CTCTCATAATATGACTATGA+TGG 0.427890 2_3:-60380014 Msa0303240:exon
GAAGGACACAACAGCATCAT+TGG 0.428454 2_3:+60379173 None:intergenic
ACACGAGTCTGAACTGTGCT+TGG 0.436375 2_3:+60379042 None:intergenic
AATGTCAAAGGTATATCAAA+TGG 0.439590 2_3:+60378645 None:intergenic
TCGACCATTAATAGCCCTAA+TGG 0.441150 2_3:+60379086 None:intergenic
GCATCATTGTTAGGCAAACT+TGG 0.445718 2_3:+60379791 None:intergenic
GGTGTCATCAACCAAGGCTT+TGG 0.445762 2_3:-60379114 Msa0303240:CDS
GTGGACCGATTCAACTATCT+TGG 0.452579 2_3:-60379263 Msa0303240:CDS
AGTGAGTTGGGTGTTGCTCC+TGG 0.457642 2_3:-60378997 Msa0303240:CDS
CAAATCACTAAGCATATGTA+TGG 0.459351 2_3:-60379987 Msa0303240:CDS
CAAGCTGATTCTAGCTGTGC+AGG 0.462495 2_3:-60379719 Msa0303240:CDS
GCTAGCTAGCCTAGCAAGTA+AGG 0.463856 2_3:+60378968 None:intergenic
CACTCAATATTGTAGTGAGT+TGG 0.467919 2_3:-60379010 Msa0303240:CDS
AAACTGATTATAAGAATTGA+GGG 0.470862 2_3:+60379661 None:intergenic
GGAGTGTGTTGCAGCCAATA+CGG 0.476582 2_3:-60379887 Msa0303240:CDS
TATTGAAGAAATCTTGTGTA+AGG 0.478357 2_3:+60379753 None:intergenic
CAAACTGATTATAAGAATTG+AGG 0.480529 2_3:+60379660 None:intergenic
CATTACCACAATACCCGTAT+TGG 0.482956 2_3:+60379873 None:intergenic
TGATGGACCATCAATCTCTT+CGG 0.485027 2_3:+60379350 None:intergenic
ACTCAATATTGTAGTGAGTT+GGG 0.486051 2_3:-60379009 Msa0303240:CDS
CAATACGGGTATTGTGGTAA+TGG 0.497456 2_3:-60379872 Msa0303240:CDS
TAGTTCCAAGATAGTTGAAT+CGG 0.500260 2_3:+60379258 None:intergenic
AACTACAACTATGGACCAGC+TGG 0.503629 2_3:-60379240 Msa0303240:CDS
AAACAAGGTCCTTGTTATGC+AGG 0.505585 2_3:-60379824 Msa0303240:CDS
TCTCATAATATGACTATGAT+GGG 0.507140 2_3:-60380013 Msa0303240:exon
CACGAGTCTGAACTGTGCTT+GGG 0.508045 2_3:+60379043 None:intergenic
ATTCTTATAATCAGTTTGGT+AGG 0.512286 2_3:-60379655 Msa0303240:CDS
TGGTGATAACCTTACTTGCT+AGG 0.514508 2_3:-60378977 Msa0303240:exon
GAGTGTGTTGCAGCCAATAC+GGG 0.522073 2_3:-60379886 Msa0303240:CDS
TAATGGTAGCACCAAAGCCT+TGG 0.534298 2_3:+60379103 None:intergenic
AAACCATTGTCTCTTCCAGC+TGG 0.544197 2_3:+60379225 None:intergenic
ATGTTAGTGCTCAAAATTGT+GGG 0.550523 2_3:-60379919 Msa0303240:CDS
AACAAAGGTTACTACGGTCG+TGG 0.552838 2_3:-60379282 Msa0303240:CDS
TGGGATGCTCAAAATGTCAA+AGG 0.554400 2_3:+60378633 None:intergenic
ATTCAAATATATACTAAATG+GGG 0.560058 2_3:+60378939 None:intergenic
TAACCTTTGTTAGGGGCACA+TGG 0.560210 2_3:+60379294 None:intergenic
ACAAGCTGAATAGACGCTAC+TGG 0.562565 2_3:+60378780 None:intergenic
TGCAGCCAATACGGGTATTG+TGG 0.572631 2_3:-60379878 Msa0303240:CDS
GGTGATGCATATTGTGGCAC+AGG 0.573444 2_3:-60379851 Msa0303240:CDS
CAAAATTGTGGGTGTGCTGA+AGG 0.577261 2_3:-60379908 Msa0303240:CDS
AATGTTAGTGCTCAAAATTG+TGG 0.577445 2_3:-60379920 Msa0303240:CDS
AGCACAGTTCAGACTCGTGT+TGG 0.581556 2_3:-60379039 Msa0303240:CDS
AGAGATTGATGGTCCATCAA+AGG 0.581585 2_3:-60379346 Msa0303240:CDS
ATGTGAGTTTGGAGTTGCAA+AGG 0.590733 2_3:+60378591 None:intergenic
TGTGGCACAGGATGCAAACA+AGG 0.594512 2_3:-60379839 Msa0303240:CDS
ATTGAACTCTCCTGAAACAG+TGG 0.597631 2_3:-60379196 Msa0303240:CDS
GCCTAACAATGATGCTAATG+TGG 0.604947 2_3:-60379783 Msa0303240:CDS
GCCCCTAACAAAGGTTACTA+CGG 0.616806 2_3:-60379288 Msa0303240:CDS
TGGCCATGTGCCCCTAACAA+AGG 0.618946 2_3:-60379297 Msa0303240:CDS
TCATTGTTAGGCAAACTTGG+TGG 0.622467 2_3:+60379794 None:intergenic
GGTGTTTGGCCTGCATAACA+AGG 0.624350 2_3:+60379815 None:intergenic
GGACCAGCTGGAAGAGACAA+TGG 0.624670 2_3:-60379228 Msa0303240:CDS
TTTGGATGACTCCAAACGTG+AGG 0.624768 2_3:-60379624 Msa0303240:CDS
TAGCAAGTAAGGTTATCACC+AGG 0.638426 2_3:+60378979 None:intergenic
GGTAATGGTGATGCATATTG+TGG 0.652622 2_3:-60379857 Msa0303240:CDS
TAGCGTCTATTCAGCTTGTG+AGG 0.653680 2_3:-60378776 Msa0303240:three_prime_UTR
AATGGTCGACTTGAATGTGA+TGG 0.664152 2_3:-60379072 Msa0303240:CDS
ACCGTAGTAACCTTTGTTAG+GGG 0.669486 2_3:+60379287 None:intergenic
GTGCACGGTGTCATCAACCA+AGG 0.695993 2_3:-60379120 Msa0303240:CDS
TATTGGATGAATAATGTGCA+CGG 0.742029 2_3:-60379135 Msa0303240:CDS
GTGATGAAAACAACACAGAG+TGG 0.752581 2_3:-60379317 Msa0303240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAATTTGTTATCAAATT+TGG - chr2_3:60379161-60379180 Msa0303240:CDS 10.0%
!! TATTCAAATATATACTAAAT+GGG + chr2_3:60379703-60379722 None:intergenic 10.0%
!! TTATTCAAATATATACTAAA+TGG + chr2_3:60379704-60379723 None:intergenic 10.0%
!!! TATTAATTTGTTATCAAATT+TGG - chr2_3:60379161-60379180 Msa0303240:CDS 10.0%
!! TATTCAAATATATACTAAAT+GGG + chr2_3:60379703-60379722 None:intergenic 10.0%
!! TTATTCAAATATATACTAAA+TGG + chr2_3:60379704-60379723 None:intergenic 10.0%
!! ATTCAAATATATACTAAATG+GGG + chr2_3:60379702-60379721 None:intergenic 15.0%
!! TAAAAATGATATCATAATGT+TGG - chr2_3:60379721-60379740 Msa0303240:CDS 15.0%
!! ATTCAAATATATACTAAATG+GGG + chr2_3:60379702-60379721 None:intergenic 15.0%
!! TAAAAATGATATCATAATGT+TGG - chr2_3:60379721-60379740 Msa0303240:CDS 15.0%
!! AACCATGATCATAATAAAAA+AGG + chr2_3:60378692-60378711 None:intergenic 20.0%
!! AAACTGATTATAAGAATTGA+GGG + chr2_3:60378980-60378999 None:intergenic 20.0%
!!! GAAATGAAATTTTTTTTGCA+AGG - chr2_3:60379916-60379935 Msa0303240:CDS 20.0%
!!! AAGACAAAAAATGGAAATTT+TGG + chr2_3:60379951-60379970 None:intergenic 20.0%
!!! TTCCATTTTTTGTCTTAATT+AGG - chr2_3:60379955-60379974 Msa0303240:CDS 20.0%
!!! TTGCAAAACTTTTTTCTTTT+GGG + chr2_3:60380027-60380046 None:intergenic 20.0%
!!! TGAAGTTAATTGTTTAAGAT+TGG + chr2_3:60378600-60378619 None:intergenic 20.0%
!! AACCATGATCATAATAAAAA+AGG + chr2_3:60378692-60378711 None:intergenic 20.0%
!! AAACTGATTATAAGAATTGA+GGG + chr2_3:60378980-60378999 None:intergenic 20.0%
!!! GAAATGAAATTTTTTTTGCA+AGG - chr2_3:60379916-60379935 Msa0303240:CDS 20.0%
!!! AAGACAAAAAATGGAAATTT+TGG + chr2_3:60379951-60379970 None:intergenic 20.0%
!!! TTCCATTTTTTGTCTTAATT+AGG - chr2_3:60379955-60379974 Msa0303240:CDS 20.0%
!!! TTGCAAAACTTTTTTCTTTT+GGG + chr2_3:60380027-60380046 None:intergenic 20.0%
! TCTCATAATATGACTATGAT+GGG - chr2_3:60378625-60378644 Msa0303240:three_prime_UTR 25.0%
!!! AGCCTTTTTTATTATGATCA+TGG - chr2_3:60378687-60378706 Msa0303240:three_prime_UTR 25.0%
!! TTTTGAGCACTAACATTTTT+TGG + chr2_3:60378715-60378734 None:intergenic 25.0%
! TATTGAAGAAATCTTGTGTA+AGG + chr2_3:60378888-60378907 None:intergenic 25.0%
! CAAACTGATTATAAGAATTG+AGG + chr2_3:60378981-60379000 None:intergenic 25.0%
! CTCAATTCTTATAATCAGTT+TGG - chr2_3:60378979-60378998 Msa0303240:CDS 25.0%
! ATTCTTATAATCAGTTTGGT+AGG - chr2_3:60378983-60379002 Msa0303240:CDS 25.0%
!!! CGTTAATTTTGTTTAGTTGT+TGG + chr2_3:60379120-60379139 None:intergenic 25.0%
! TGCCTAATTAAGACAAAAAA+TGG + chr2_3:60379960-60379979 None:intergenic 25.0%
!! AATGTCAAAGGTATATCAAA+TGG + chr2_3:60379996-60380015 None:intergenic 25.0%
!!! GTTGCAAAACTTTTTTCTTT+TGG + chr2_3:60380028-60380047 None:intergenic 25.0%
! TCTCATAATATGACTATGAT+GGG - chr2_3:60378625-60378644 Msa0303240:three_prime_UTR 25.0%
!!! AGCCTTTTTTATTATGATCA+TGG - chr2_3:60378687-60378706 Msa0303240:three_prime_UTR 25.0%
!! TTTTGAGCACTAACATTTTT+TGG + chr2_3:60378715-60378734 None:intergenic 25.0%
! TATTGAAGAAATCTTGTGTA+AGG + chr2_3:60378888-60378907 None:intergenic 25.0%
! CAAACTGATTATAAGAATTG+AGG + chr2_3:60378981-60379000 None:intergenic 25.0%
! CTCAATTCTTATAATCAGTT+TGG - chr2_3:60378979-60378998 Msa0303240:CDS 25.0%
! ATTCTTATAATCAGTTTGGT+AGG - chr2_3:60378983-60379002 Msa0303240:CDS 25.0%
!!! CGTTAATTTTGTTTAGTTGT+TGG + chr2_3:60379120-60379139 None:intergenic 25.0%
! TGCCTAATTAAGACAAAAAA+TGG + chr2_3:60379960-60379979 None:intergenic 25.0%
!! AATGTCAAAGGTATATCAAA+TGG + chr2_3:60379996-60380015 None:intergenic 25.0%
!!! GTTGCAAAACTTTTTTCTTT+TGG + chr2_3:60380028-60380047 None:intergenic 25.0%
CTCTCATAATATGACTATGA+TGG - chr2_3:60378624-60378643 Msa0303240:three_prime_UTR 30.0%
CAAATCACTAAGCATATGTA+TGG - chr2_3:60378651-60378670 Msa0303240:three_prime_UTR 30.0%
AATGTTAGTGCTCAAAATTG+TGG - chr2_3:60378718-60378737 Msa0303240:three_prime_UTR 30.0%
ATGTTAGTGCTCAAAATTGT+GGG - chr2_3:60378719-60378738 Msa0303240:three_prime_UTR 30.0%
AAATTTGGTTACACAACTGT+TGG - chr2_3:60379176-60379195 Msa0303240:CDS 30.0%
AATTTGGTTACACAACTGTT+GGG - chr2_3:60379177-60379196 Msa0303240:CDS 30.0%
TAGTTCCAAGATAGTTGAAT+CGG + chr2_3:60379383-60379402 None:intergenic 30.0%
TATTGGATGAATAATGTGCA+CGG - chr2_3:60379503-60379522 Msa0303240:intron 30.0%
ACTCAATATTGTAGTGAGTT+GGG - chr2_3:60379629-60379648 Msa0303240:CDS 30.0%
TATATACTAAATGGGGTGTA+TGG + chr2_3:60379695-60379714 None:intergenic 30.0%
GACAAATTGGTTACAAGATA+TGG - chr2_3:60379894-60379913 Msa0303240:CDS 30.0%
CTCTCATAATATGACTATGA+TGG - chr2_3:60378624-60378643 Msa0303240:three_prime_UTR 30.0%
CAAATCACTAAGCATATGTA+TGG - chr2_3:60378651-60378670 Msa0303240:three_prime_UTR 30.0%
AATGTTAGTGCTCAAAATTG+TGG - chr2_3:60378718-60378737 Msa0303240:three_prime_UTR 30.0%
ATGTTAGTGCTCAAAATTGT+GGG - chr2_3:60378719-60378738 Msa0303240:three_prime_UTR 30.0%
AAATTTGGTTACACAACTGT+TGG - chr2_3:60379176-60379195 Msa0303240:CDS 30.0%
AATTTGGTTACACAACTGTT+GGG - chr2_3:60379177-60379196 Msa0303240:CDS 30.0%
TAGTTCCAAGATAGTTGAAT+CGG + chr2_3:60379383-60379402 None:intergenic 30.0%
TATTGGATGAATAATGTGCA+CGG - chr2_3:60379503-60379522 Msa0303240:intron 30.0%
ACTCAATATTGTAGTGAGTT+GGG - chr2_3:60379629-60379648 Msa0303240:CDS 30.0%
TATATACTAAATGGGGTGTA+TGG + chr2_3:60379695-60379714 None:intergenic 30.0%
GACAAATTGGTTACAAGATA+TGG - chr2_3:60379894-60379913 Msa0303240:CDS 30.0%
TATAATCAGTTTGGTAGGTC+TGG - chr2_3:60378988-60379007 Msa0303240:CDS 35.0%
CTATCTTGGAACTACAACTA+TGG - chr2_3:60379389-60379408 Msa0303240:intron 35.0%
ATACCATAATGCTGTCTTGA+AGG + chr2_3:60379486-60379505 None:intergenic 35.0%
TCAAGACAGCATTATGGTAT+TGG - chr2_3:60379486-60379505 Msa0303240:intron 35.0%
CACTCAATATTGTAGTGAGT+TGG - chr2_3:60379628-60379647 Msa0303240:CDS 35.0%
TATAATCAGTTTGGTAGGTC+TGG - chr2_3:60378988-60379007 Msa0303240:CDS 35.0%
CTATCTTGGAACTACAACTA+TGG - chr2_3:60379389-60379408 Msa0303240:intron 35.0%
ATACCATAATGCTGTCTTGA+AGG + chr2_3:60379486-60379505 None:intergenic 35.0%
TCAAGACAGCATTATGGTAT+TGG - chr2_3:60379486-60379505 Msa0303240:intron 35.0%
CACTCAATATTGTAGTGAGT+TGG - chr2_3:60379628-60379647 Msa0303240:CDS 35.0%
CATTACCACAATACCCGTAT+TGG + chr2_3:60378768-60378787 None:intergenic 40.0%
! CAATACGGGTATTGTGGTAA+TGG - chr2_3:60378766-60378785 Msa0303240:three_prime_UTR 40.0%
! GGTAATGGTGATGCATATTG+TGG - chr2_3:60378781-60378800 Msa0303240:three_prime_UTR 40.0%
! AAACAAGGTCCTTGTTATGC+AGG - chr2_3:60378814-60378833 Msa0303240:three_prime_UTR 40.0%
TCATTGTTAGGCAAACTTGG+TGG + chr2_3:60378847-60378866 None:intergenic 40.0%
GCATCATTGTTAGGCAAACT+TGG + chr2_3:60378850-60378869 None:intergenic 40.0%
GCCTAACAATGATGCTAATG+TGG - chr2_3:60378855-60378874 Msa0303240:three_prime_UTR 40.0%
GCCACATTAGCATCATTGTT+AGG + chr2_3:60378859-60378878 None:intergenic 40.0%
GCTCATTTCACACATGAAAC+TGG - chr2_3:60379051-60379070 Msa0303240:CDS 40.0%
TGCTATACCGAAGAGATTGA+TGG - chr2_3:60379281-60379300 Msa0303240:CDS 40.0%
! TGATGGACCATCAATCTCTT+CGG + chr2_3:60379291-60379310 None:intergenic 40.0%
! AGAGATTGATGGTCCATCAA+AGG - chr2_3:60379292-60379311 Msa0303240:CDS 40.0%
TCATCACAGTAGTCCTTTGA+TGG + chr2_3:60379308-60379327 None:intergenic 40.0%
GTGATGAAAACAACACAGAG+TGG - chr2_3:60379321-60379340 Msa0303240:CDS 40.0%
ACCGTAGTAACCTTTGTTAG+GGG + chr2_3:60379354-60379373 None:intergenic 40.0%
GACCGTAGTAACCTTTGTTA+GGG + chr2_3:60379355-60379374 None:intergenic 40.0%
!!! GGAAGAGACAATGGTTTTGA+TGG - chr2_3:60379419-60379438 Msa0303240:intron 40.0%
ATTGAACTCTCCTGAAACAG+TGG - chr2_3:60379442-60379461 Msa0303240:intron 40.0%
TGTCCTTCAAGACAGCATTA+TGG - chr2_3:60379480-60379499 Msa0303240:intron 40.0%
GCTACCATTAGGGCTATTAA+TGG - chr2_3:60379548-60379567 Msa0303240:intron 40.0%
TCGACCATTAATAGCCCTAA+TGG + chr2_3:60379555-60379574 None:intergenic 40.0%
!! AATGGTCGACTTGAATGTGA+TGG - chr2_3:60379566-60379585 Msa0303240:intron 40.0%
TAGCAAGTAAGGTTATCACC+AGG + chr2_3:60379662-60379681 None:intergenic 40.0%
TGGTGATAACCTTACTTGCT+AGG - chr2_3:60379661-60379680 Msa0303240:CDS 40.0%
TGGGATGCTCAAAATGTCAA+AGG + chr2_3:60380008-60380027 None:intergenic 40.0%
CATTACCACAATACCCGTAT+TGG + chr2_3:60378768-60378787 None:intergenic 40.0%
! CAATACGGGTATTGTGGTAA+TGG - chr2_3:60378766-60378785 Msa0303240:three_prime_UTR 40.0%
! GGTAATGGTGATGCATATTG+TGG - chr2_3:60378781-60378800 Msa0303240:three_prime_UTR 40.0%
! AAACAAGGTCCTTGTTATGC+AGG - chr2_3:60378814-60378833 Msa0303240:three_prime_UTR 40.0%
TCATTGTTAGGCAAACTTGG+TGG + chr2_3:60378847-60378866 None:intergenic 40.0%
GCATCATTGTTAGGCAAACT+TGG + chr2_3:60378850-60378869 None:intergenic 40.0%
GCCTAACAATGATGCTAATG+TGG - chr2_3:60378855-60378874 Msa0303240:three_prime_UTR 40.0%
GCCACATTAGCATCATTGTT+AGG + chr2_3:60378859-60378878 None:intergenic 40.0%
GCTCATTTCACACATGAAAC+TGG - chr2_3:60379051-60379070 Msa0303240:CDS 40.0%
TGCTATACCGAAGAGATTGA+TGG - chr2_3:60379281-60379300 Msa0303240:CDS 40.0%
! TGATGGACCATCAATCTCTT+CGG + chr2_3:60379291-60379310 None:intergenic 40.0%
! AGAGATTGATGGTCCATCAA+AGG - chr2_3:60379292-60379311 Msa0303240:CDS 40.0%
TCATCACAGTAGTCCTTTGA+TGG + chr2_3:60379308-60379327 None:intergenic 40.0%
GTGATGAAAACAACACAGAG+TGG - chr2_3:60379321-60379340 Msa0303240:CDS 40.0%
ACCGTAGTAACCTTTGTTAG+GGG + chr2_3:60379354-60379373 None:intergenic 40.0%
GACCGTAGTAACCTTTGTTA+GGG + chr2_3:60379355-60379374 None:intergenic 40.0%
!!! GGAAGAGACAATGGTTTTGA+TGG - chr2_3:60379419-60379438 Msa0303240:intron 40.0%
ATTGAACTCTCCTGAAACAG+TGG - chr2_3:60379442-60379461 Msa0303240:intron 40.0%
TGTCCTTCAAGACAGCATTA+TGG - chr2_3:60379480-60379499 Msa0303240:intron 40.0%
GCTACCATTAGGGCTATTAA+TGG - chr2_3:60379548-60379567 Msa0303240:intron 40.0%
TCGACCATTAATAGCCCTAA+TGG + chr2_3:60379555-60379574 None:intergenic 40.0%
!! AATGGTCGACTTGAATGTGA+TGG - chr2_3:60379566-60379585 Msa0303240:intron 40.0%
TAGCAAGTAAGGTTATCACC+AGG + chr2_3:60379662-60379681 None:intergenic 40.0%
TGGTGATAACCTTACTTGCT+AGG - chr2_3:60379661-60379680 Msa0303240:CDS 40.0%
TGGGATGCTCAAAATGTCAA+AGG + chr2_3:60380008-60380027 None:intergenic 40.0%
CAAAATTGTGGGTGTGCTGA+AGG - chr2_3:60378730-60378749 Msa0303240:three_prime_UTR 45.0%
!! TAGGCAAACTTGGTGGTGTT+TGG + chr2_3:60378840-60378859 None:intergenic 45.0%
!! GTTTGGTAGGTCTGGTTCTT+TGG - chr2_3:60378996-60379015 Msa0303240:CDS 45.0%
TTTGGATGACTCCAAACGTG+AGG - chr2_3:60379014-60379033 Msa0303240:CDS 45.0%
! TAACCTTTGTTAGGGGCACA+TGG + chr2_3:60379347-60379366 None:intergenic 45.0%
GCCCCTAACAAAGGTTACTA+CGG - chr2_3:60379350-60379369 Msa0303240:CDS 45.0%
CGACCGTAGTAACCTTTGTT+AGG + chr2_3:60379356-60379375 None:intergenic 45.0%
AACAAAGGTTACTACGGTCG+TGG - chr2_3:60379356-60379375 Msa0303240:CDS 45.0%
! GTGGACCGATTCAACTATCT+TGG - chr2_3:60379375-60379394 Msa0303240:intron 45.0%
AACTACAACTATGGACCAGC+TGG - chr2_3:60379398-60379417 Msa0303240:intron 45.0%
AAACCATTGTCTCTTCCAGC+TGG + chr2_3:60379416-60379435 None:intergenic 45.0%
GAAGGACACAACAGCATCAT+TGG + chr2_3:60379468-60379487 None:intergenic 45.0%
TAATGGTAGCACCAAAGCCT+TGG + chr2_3:60379538-60379557 None:intergenic 45.0%
!! AAGGCTTTGGTGCTACCATT+AGG - chr2_3:60379537-60379556 Msa0303240:intron 45.0%
!! AGGCTTTGGTGCTACCATTA+GGG - chr2_3:60379538-60379557 Msa0303240:intron 45.0%
ACAAGCTGAATAGACGCTAC+TGG + chr2_3:60379861-60379880 None:intergenic 45.0%
TAGCGTCTATTCAGCTTGTG+AGG - chr2_3:60379862-60379881 Msa0303240:CDS 45.0%
GAGGCACACGTTTGACAAAT+TGG - chr2_3:60379881-60379900 Msa0303240:CDS 45.0%
CAAAATTGTGGGTGTGCTGA+AGG - chr2_3:60378730-60378749 Msa0303240:three_prime_UTR 45.0%
!! TAGGCAAACTTGGTGGTGTT+TGG + chr2_3:60378840-60378859 None:intergenic 45.0%
!! GTTTGGTAGGTCTGGTTCTT+TGG - chr2_3:60378996-60379015 Msa0303240:CDS 45.0%
TTTGGATGACTCCAAACGTG+AGG - chr2_3:60379014-60379033 Msa0303240:CDS 45.0%
! TAACCTTTGTTAGGGGCACA+TGG + chr2_3:60379347-60379366 None:intergenic 45.0%
GCCCCTAACAAAGGTTACTA+CGG - chr2_3:60379350-60379369 Msa0303240:CDS 45.0%
CGACCGTAGTAACCTTTGTT+AGG + chr2_3:60379356-60379375 None:intergenic 45.0%
AACAAAGGTTACTACGGTCG+TGG - chr2_3:60379356-60379375 Msa0303240:CDS 45.0%
! GTGGACCGATTCAACTATCT+TGG - chr2_3:60379375-60379394 Msa0303240:intron 45.0%
AACTACAACTATGGACCAGC+TGG - chr2_3:60379398-60379417 Msa0303240:intron 45.0%
AAACCATTGTCTCTTCCAGC+TGG + chr2_3:60379416-60379435 None:intergenic 45.0%
GAAGGACACAACAGCATCAT+TGG + chr2_3:60379468-60379487 None:intergenic 45.0%
TAATGGTAGCACCAAAGCCT+TGG + chr2_3:60379538-60379557 None:intergenic 45.0%
!! AAGGCTTTGGTGCTACCATT+AGG - chr2_3:60379537-60379556 Msa0303240:intron 45.0%
!! AGGCTTTGGTGCTACCATTA+GGG - chr2_3:60379538-60379557 Msa0303240:intron 45.0%
ACAAGCTGAATAGACGCTAC+TGG + chr2_3:60379861-60379880 None:intergenic 45.0%
TAGCGTCTATTCAGCTTGTG+AGG - chr2_3:60379862-60379881 Msa0303240:CDS 45.0%
GAGGCACACGTTTGACAAAT+TGG - chr2_3:60379881-60379900 Msa0303240:CDS 45.0%
GGAGTGTGTTGCAGCCAATA+CGG - chr2_3:60378751-60378770 Msa0303240:three_prime_UTR 50.0%
GAGTGTGTTGCAGCCAATAC+GGG - chr2_3:60378752-60378771 Msa0303240:three_prime_UTR 50.0%
! TGCAGCCAATACGGGTATTG+TGG - chr2_3:60378760-60378779 Msa0303240:three_prime_UTR 50.0%
GGTGATGCATATTGTGGCAC+AGG - chr2_3:60378787-60378806 Msa0303240:three_prime_UTR 50.0%
TGTGGCACAGGATGCAAACA+AGG - chr2_3:60378799-60378818 Msa0303240:three_prime_UTR 50.0%
GGTGTTTGGCCTGCATAACA+AGG + chr2_3:60378826-60378845 None:intergenic 50.0%
! CAAGCTGATTCTAGCTGTGC+AGG - chr2_3:60378919-60378938 Msa0303240:three_prime_UTR 50.0%
GCATCATTGGCCACTGTTTC+AGG + chr2_3:60379455-60379474 None:intergenic 50.0%
! GGTGTCATCAACCAAGGCTT+TGG - chr2_3:60379524-60379543 Msa0303240:intron 50.0%
CACGAGTCTGAACTGTGCTT+GGG + chr2_3:60379598-60379617 None:intergenic 50.0%
ACACGAGTCTGAACTGTGCT+TGG + chr2_3:60379599-60379618 None:intergenic 50.0%
AGCACAGTTCAGACTCGTGT+TGG - chr2_3:60379599-60379618 Msa0303240:CDS 50.0%
! GCTAGCTAGCCTAGCAAGTA+AGG + chr2_3:60379673-60379692 None:intergenic 50.0%
GGAGTGTGTTGCAGCCAATA+CGG - chr2_3:60378751-60378770 Msa0303240:three_prime_UTR 50.0%
GAGTGTGTTGCAGCCAATAC+GGG - chr2_3:60378752-60378771 Msa0303240:three_prime_UTR 50.0%
! TGCAGCCAATACGGGTATTG+TGG - chr2_3:60378760-60378779 Msa0303240:three_prime_UTR 50.0%
GGTGATGCATATTGTGGCAC+AGG - chr2_3:60378787-60378806 Msa0303240:three_prime_UTR 50.0%
TGTGGCACAGGATGCAAACA+AGG - chr2_3:60378799-60378818 Msa0303240:three_prime_UTR 50.0%
GGTGTTTGGCCTGCATAACA+AGG + chr2_3:60378826-60378845 None:intergenic 50.0%
! CAAGCTGATTCTAGCTGTGC+AGG - chr2_3:60378919-60378938 Msa0303240:three_prime_UTR 50.0%
GCATCATTGGCCACTGTTTC+AGG + chr2_3:60379455-60379474 None:intergenic 50.0%
! GGTGTCATCAACCAAGGCTT+TGG - chr2_3:60379524-60379543 Msa0303240:intron 50.0%
CACGAGTCTGAACTGTGCTT+GGG + chr2_3:60379598-60379617 None:intergenic 50.0%
ACACGAGTCTGAACTGTGCT+TGG + chr2_3:60379599-60379618 None:intergenic 50.0%
AGCACAGTTCAGACTCGTGT+TGG - chr2_3:60379599-60379618 Msa0303240:CDS 50.0%
! GCTAGCTAGCCTAGCAAGTA+AGG + chr2_3:60379673-60379692 None:intergenic 50.0%
AGCAGCTGCAACCTCACGTT+TGG + chr2_3:60379028-60379047 None:intergenic 55.0%
TGGCCATGTGCCCCTAACAA+AGG - chr2_3:60379341-60379360 Msa0303240:CDS 55.0%
GGACCAGCTGGAAGAGACAA+TGG - chr2_3:60379410-60379429 Msa0303240:intron 55.0%
!! GTGCACGGTGTCATCAACCA+AGG - chr2_3:60379518-60379537 Msa0303240:intron 55.0%
!! AGTGAGTTGGGTGTTGCTCC+TGG - chr2_3:60379641-60379660 Msa0303240:CDS 55.0%
AGCAGCTGCAACCTCACGTT+TGG + chr2_3:60379028-60379047 None:intergenic 55.0%
TGGCCATGTGCCCCTAACAA+AGG - chr2_3:60379341-60379360 Msa0303240:CDS 55.0%
GGACCAGCTGGAAGAGACAA+TGG - chr2_3:60379410-60379429 Msa0303240:intron 55.0%
!! GTGCACGGTGTCATCAACCA+AGG - chr2_3:60379518-60379537 Msa0303240:intron 55.0%
!! AGTGAGTTGGGTGTTGCTCC+TGG - chr2_3:60379641-60379660 Msa0303240:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 60378600 60380060 60378600 ID=Msa0303240;Name=Msa0303240
chr2_3 mRNA 60378600 60380060 60378600 ID=Msa0303240-mRNA-1;Parent=Msa0303240;Name=Msa0303240-mRNA-1;_AED=0.28;_eAED=0.28;_QI=34|1|1|1|1|1|2|378|282
chr2_3 exon 60378600 60379384 60378600 ID=Msa0303240-mRNA-1:exon:19675;Parent=Msa0303240-mRNA-1
chr2_3 exon 60379585 60380060 60379585 ID=Msa0303240-mRNA-1:exon:19674;Parent=Msa0303240-mRNA-1
chr2_3 five_prime_UTR 60380027 60380060 60380027 ID=Msa0303240-mRNA-1:five_prime_utr;Parent=Msa0303240-mRNA-1
chr2_3 CDS 60379585 60380026 60379585 ID=Msa0303240-mRNA-1:cds;Parent=Msa0303240-mRNA-1
chr2_3 CDS 60378978 60379384 60378978 ID=Msa0303240-mRNA-1:cds;Parent=Msa0303240-mRNA-1
chr2_3 three_prime_UTR 60378600 60378977 60378600 ID=Msa0303240-mRNA-1:three_prime_utr;Parent=Msa0303240-mRNA-1
Gene Sequence

>Msa0303240

ATGACTATGATGGGAAACAAATCACTAAGCATATGTATGGCAACACTTGCTATAGCCTTTTTTATTATGATCATGGTTCCAAAAAATGTTAGTGCTCAAAATTGTGGGTGTGCTGAAGGAGTGTGTTGCAGCCAATACGGGTATTGTGGTAATGGTGATGCATATTGTGGCACAGGATGCAAACAAGGTCCTTGTTATGCAGGCCAAACACCACCAAGTTTGCCTAACAATGATGCTAATGTGGCTGACATCCTTACACAAGATTTCTTCAATAGTATAATTGATCAAGCTGATTCTAGCTGTGCAGGAAAGAACTTCTACACAAGAGCTGCTTTTCTTGATGCCCTCAATTCTTATAATCAGTTTGGTAGGTCTGGTTCTTTGGATGACTCCAAACGTGAGGTTGCAGCTGCTTTTGCTCATTTCACACATGAAACTGGACATTTTTGCTATACCGAAGAGATTGATGGTCCATCAAAGGACTACTGTGATGAAAACAACACAGAGTGGCCATGTGCCCCTAACAAAGGTTACTACGGTCGTGGACCGATTCAACTATCTTGGAACTACAACTATGGACCAGCTGGAAGAGACAATGGTTTTGATGGATTGAACTCTCCTGAAACAGTGGCCAATGATGCTGTTGTGTCCTTCAAGACAGCATTATGGTATTGGATGAATAATGTGCACGGTGTCATCAACCAAGGCTTTGGTGCTACCATTAGGGCTATTAATGGTCGACTTGAATGTGATGGTGCTAACCCAAGCACAGTTCAGACTCGTGTTGGTTACTACACTCAATATTGTAGTGAGTTGGGTGTTGCTCCTGGTGATAACCTTACTTGCTAG
Protein sequence

>Msa0303240

MTMMGNKSLSICMATLAIAFFIMIMVPKNVSAQNCGCAEGVCCSQYGYCGNGDAYCGTGCKQGPCYAGQTPPSLPNNDANVADILTQDFFNSIIDQADSSCAGKNFYTRAAFLDALNSYNQFGRSGSLDDSKREVAAAFAHFTHETGHFCYTEEIDGPSKDYCDENNTEWPCAPNKGYYGRGPIQLSWNYNYGPAGRDNGFDGLNSPETVANDAVVSFKTALWYWMNNVHGVINQGFGATIRAINGRLECDGANPSTVQTRVGYYTQYCSELGVAPGDNLTC*