AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0827240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827240 MtrunA17_Chr2g0329791 98.227 282 5 0 1 282 1 282 0.0 578
Msa0827240 MtrunA17_Chr2g0329781 81.890 254 43 2 32 282 10 263 3.84e-158 441
Msa0827240 MtrunA17_Chr3g0145981 35.759 316 149 8 9 282 22 325 4.11e-59 192
Msa0827240 MtrunA17_Chr8g0370851 36.957 322 151 9 4 282 1 313 5.04e-58 188
Msa0827240 MtrunA17_Chr8g0370821 37.584 298 137 9 30 282 31 324 4.87e-54 178
Msa0827240 MtrunA17_Chr7g0270841 39.241 237 108 6 81 282 36 271 1.13e-53 176
Msa0827240 MtrunA17_Chr8g0370831 37.282 287 140 9 31 282 21 302 3.39e-47 160
Msa0827240 MtrunA17_Chr2g0329771 80.000 80 16 0 1 80 1 80 1.03e-42 141
Msa0827240 MtrunA17_Chr2g0329761 83.019 53 9 0 229 281 3 55 1.48e-24 93.6
Msa0827240 MtrunA17_Chr2g0294721 31.120 241 121 8 86 282 62 301 3.52e-24 99.4
Msa0827240 MtrunA17_Chr1g0206111 27.273 253 131 7 78 282 60 307 1.20e-20 89.7
Msa0827240 MtrunA17_Chr7g0221751 25.714 315 171 12 14 282 7 304 3.81e-19 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0827240 AT3G54420.1 66.312 282 85 3 1 282 2 273 1.04e-136 387
Msa0827240 AT2G43590.1 56.863 255 100 3 28 282 20 264 3.33e-106 309
Msa0827240 AT2G43580.1 52.941 255 111 3 28 282 20 265 9.93e-97 285
Msa0827240 AT2G43570.1 50.704 284 119 8 6 282 8 277 6.80e-90 268
Msa0827240 AT2G43620.1 45.683 278 130 8 18 282 14 283 7.15e-83 251
Msa0827240 AT2G43610.1 44.718 284 138 7 10 282 6 281 1.53e-82 250
Msa0827240 AT3G47540.1 45.982 224 98 5 60 282 13 214 6.02e-62 195
Msa0827240 AT1G56680.1 39.068 279 150 11 15 282 11 280 1.52e-59 191
Msa0827240 AT1G56680.2 39.068 279 150 11 15 282 34 303 3.45e-59 191
Msa0827240 AT3G47540.2 44.796 221 99 5 60 279 13 211 1.97e-57 186
Msa0827240 AT3G12500.1 39.286 280 132 6 37 282 45 320 3.12e-55 182
Msa0827240 AT2G43600.1 35.563 284 160 11 11 282 1 273 7.09e-54 176
Msa0827240 AT2G43600.2 35.439 285 161 11 10 282 21 294 1.78e-53 176
Msa0827240 AT1G02360.1 39.916 238 107 5 80 282 29 265 2.14e-53 175
Msa0827240 AT1G02360.3 39.916 238 107 5 80 282 81 317 4.73e-53 176
Msa0827240 AT1G02360.2 39.916 238 107 5 80 282 75 311 5.57e-53 175
Msa0827240 AT4G01700.1 38.912 239 110 5 79 282 36 273 7.07e-50 166
Msa0827240 AT1G05850.1 22.500 320 175 10 11 282 10 304 1.55e-20 89.7
Msa0827240 AT1G05850.2 22.500 320 175 10 11 282 10 304 1.55e-20 89.7
Msa0827240 AT3G16920.2 27.530 247 133 9 81 282 92 337 2.09e-19 87.0
Msa0827240 AT3G16920.1 27.530 247 133 9 81 282 68 313 2.21e-19 86.7

Find 77 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCATTGGCCACTGTTTC+AGG 0.200039 5_3:+68396562 None:intergenic
GTTTGGTAGGTCTGGTTCTT+TGG 0.220014 5_3:-68397033 Msa0827240:CDS
CGACCGTAGTAACCTTTGTT+AGG 0.286313 5_3:+68396661 None:intergenic
TTAAAATTGTATTATTGAAT+TGG 0.295578 5_3:+68396202 None:intergenic
CTATCTTGGAACTACAACTA+TGG 0.300245 5_3:-68396625 Msa0827240:CDS
TCATCACAGTAGTCCTTTGA+TGG 0.329355 5_3:+68396709 None:intergenic
AAGGCTTTGGTGCAACCATT+AGG 0.341342 5_3:-68396477 Msa0827240:CDS
CTCAATTCTTATAATCAGTT+TGG 0.343244 5_3:-68397050 Msa0827240:CDS
TATAATCAGTTTGGTAGGTC+TGG 0.352546 5_3:-68397041 Msa0827240:CDS
TCAAGACAGCATTATGGTAT+TGG 0.355700 5_3:-68396528 Msa0827240:CDS
GCTCATTTCACACATGAAAC+TGG 0.365058 5_3:-68396978 Msa0827240:CDS
AAATGATATCATCATAATAC+TGG 0.374440 5_3:-68396287 Msa0827240:three_prime_UTR
GCAACCATTAGGGCTATTAA+TGG 0.385855 5_3:-68396466 Msa0827240:CDS
TGTCCTTCAAGACAGCATTA+TGG 0.387076 5_3:-68396534 Msa0827240:CDS
TGCTATACCGAAGAGATTGA+TGG 0.389108 5_3:-68396733 Msa0827240:CDS
AGCAGCTGCAACCTCACGTT+TGG 0.392131 5_3:+68397004 None:intergenic
ATACCATAATGCTGTCTTGA+AGG 0.394568 5_3:+68396531 None:intergenic
AGGCTTTGGTGCAACCATTA+GGG 0.395270 5_3:-68396476 Msa0827240:CDS
TAGGCAAACTTGGTGGTGTT+TGG 0.406151 5_3:+68397192 None:intergenic
GCCACATTAGCATCATTGTT+AGG 0.421338 5_3:+68397173 None:intergenic
GACCGTAGTAACCTTTGTTA+GGG 0.424083 5_3:+68396662 None:intergenic
CTCTCATAATATGACTATGA+TGG 0.427890 5_3:-68397405 Msa0827240:exon
GAAGGACACAACAGCATCAT+TGG 0.428454 5_3:+68396549 None:intergenic
CAATACGGGTATTGCGGTAA+TGG 0.430986 5_3:-68397263 Msa0827240:CDS
TCGACCATTAATAGCCCTAA+TGG 0.441150 5_3:+68396462 None:intergenic
TATATACTAAATGTGTTGTA+TGG 0.442160 5_3:+68396322 None:intergenic
GCATCATTGTTAGGCAAACT+TGG 0.445718 5_3:+68397182 None:intergenic
GGTGTCATCAACCAAGGCTT+TGG 0.445762 5_3:-68396490 Msa0827240:CDS
GCTAGCTAGCTTAGCAAGTA+AGG 0.447017 5_3:+68396344 None:intergenic
GTGGACCGATTCAACTATCT+TGG 0.452579 5_3:-68396639 Msa0827240:CDS
AGTGAGTTGGGTGTTGCTCC+TGG 0.457642 5_3:-68396373 Msa0827240:CDS
CAAATCACTAAGCATATGTA+TGG 0.459351 5_3:-68397378 Msa0827240:CDS
CAAGCTGATTCTAGCTGTGC+AGG 0.462495 5_3:-68397110 Msa0827240:CDS
CACTCAATATTGTAGTGAGT+TGG 0.467919 5_3:-68396386 Msa0827240:CDS
AAACTGATTATAAGAATTGA+GGG 0.470862 5_3:+68397052 None:intergenic
GGAGTGTGTTGCAGCCAATA+CGG 0.476582 5_3:-68397278 Msa0827240:CDS
TATTGAAGAAATCTTGTGTA+AGG 0.478357 5_3:+68397144 None:intergenic
CAAACTGATTATAAGAATTG+AGG 0.480529 5_3:+68397051 None:intergenic
CATTACCGCAATACCCGTAT+TGG 0.482956 5_3:+68397264 None:intergenic
TGATGGACCATCAATCTCTT+CGG 0.485027 5_3:+68396726 None:intergenic
ACTCAATATTGTAGTGAGTT+GGG 0.486051 5_3:-68396385 Msa0827240:CDS
TAGTTCCAAGATAGTTGAAT+CGG 0.500260 5_3:+68396634 None:intergenic
TAATGGTTGCACCAAAGCCT+TGG 0.502879 5_3:+68396479 None:intergenic
AACTACAACTATGGACCAGC+TGG 0.503629 5_3:-68396616 Msa0827240:CDS
AAACAAGGTCCTTGTTATGC+AGG 0.505585 5_3:-68397215 Msa0827240:CDS
TCTCATAATATGACTATGAT+GGG 0.507140 5_3:-68397404 Msa0827240:exon
ATTCTTATAATCAGTTTGGT+AGG 0.512286 5_3:-68397046 Msa0827240:CDS
ACACGAGTCTGAACTGTGGT+TGG 0.517796 5_3:+68396418 None:intergenic
GAGTGTGTTGCAGCCAATAC+GGG 0.522073 5_3:-68397277 Msa0827240:CDS
CACGAGTCTGAACTGTGGTT+GGG 0.536022 5_3:+68396419 None:intergenic
AAACCATTGTCTCTTCCAGC+TGG 0.544197 5_3:+68396601 None:intergenic
ATGTTAGTGCTCAAAATTGT+GGG 0.550523 5_3:-68397310 Msa0827240:CDS
AACAAAGGTTACTACGGTCG+TGG 0.552838 5_3:-68396658 Msa0827240:CDS
TAACCTTTGTTAGGGGCACA+TGG 0.560210 5_3:+68396670 None:intergenic
GGTGATGCATATTGTGGCAC+AGG 0.573444 5_3:-68397242 Msa0827240:CDS
CAAAATTGTGGGTGTGCTGA+AGG 0.577261 5_3:-68397299 Msa0827240:CDS
AATGTTAGTGCTCAAAATTG+TGG 0.577445 5_3:-68397311 Msa0827240:CDS
AGAGATTGATGGTCCATCAA+AGG 0.581585 5_3:-68396722 Msa0827240:CDS
ACCACAGTTCAGACTCGTGT+TGG 0.593974 5_3:-68396415 Msa0827240:CDS
TGTGGCACAGGATGCAAACA+AGG 0.594512 5_3:-68397230 Msa0827240:CDS
ATTGAACTCTCCTGAAACAG+TGG 0.597631 5_3:-68396572 Msa0827240:CDS
TGCAGCCAATACGGGTATTG+CGG 0.599533 5_3:-68397269 Msa0827240:CDS
GCCTAACAATGATGCTAATG+TGG 0.604947 5_3:-68397174 Msa0827240:CDS
GCCCCTAACAAAGGTTACTA+CGG 0.616806 5_3:-68396664 Msa0827240:CDS
TGGCCATGTGCCCCTAACAA+AGG 0.618946 5_3:-68396673 Msa0827240:CDS
TCATTGTTAGGCAAACTTGG+TGG 0.622467 5_3:+68397185 None:intergenic
GGTGTTTGGCCTGCATAACA+AGG 0.624350 5_3:+68397206 None:intergenic
GGACCAGCTGGAAGAGACAA+TGG 0.624670 5_3:-68396604 Msa0827240:CDS
TTTGGATGACTCCAAACGTG+AGG 0.624768 5_3:-68397015 Msa0827240:CDS
TAGCAAGTAAGGTTATCACC+AGG 0.638426 5_3:+68396355 None:intergenic
AATGGTCGACTTGAATGTGA+TGG 0.664152 5_3:-68396448 Msa0827240:CDS
GGTAATGGTGATGCATATTG+TGG 0.666054 5_3:-68397248 Msa0827240:CDS
ACCGTAGTAACCTTTGTTAG+GGG 0.669486 5_3:+68396663 None:intergenic
GTGCACGGTGTCATCAACCA+AGG 0.695993 5_3:-68396496 Msa0827240:CDS
TATTGGATGAATAATGTGCA+CGG 0.742029 5_3:-68396511 Msa0827240:CDS
GTGATGAAAACAACACAGAG+TGG 0.752581 5_3:-68396693 Msa0827240:CDS
ACCAACACGAGTCTGAACTG+TGG 0.761414 5_3:+68396414 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAATTTGTTATCAAATT+TGG - chr5_3:68396772-68396791 Msa0827240:intron 10.0%
!!! TATTAATTTGTTATCAAATT+TGG - chr5_3:68396772-68396791 Msa0827240:intron 10.0%
!! AACCATGATCATAATAAAAA+AGG + chr5_3:68396303-68396322 None:intergenic 20.0%
!! AAACTGATTATAAGAATTGA+GGG + chr5_3:68396591-68396610 None:intergenic 20.0%
!! TATATACTAAATGTGTTGTA+TGG + chr5_3:68397321-68397340 None:intergenic 20.0%
!! AAATGATATCATCATAATAC+TGG - chr5_3:68397353-68397372 Msa0827240:CDS 20.0%
!! AACCATGATCATAATAAAAA+AGG + chr5_3:68396303-68396322 None:intergenic 20.0%
!! AAACTGATTATAAGAATTGA+GGG + chr5_3:68396591-68396610 None:intergenic 20.0%
!! TATATACTAAATGTGTTGTA+TGG + chr5_3:68397321-68397340 None:intergenic 20.0%
!! AAATGATATCATCATAATAC+TGG - chr5_3:68397353-68397372 Msa0827240:CDS 20.0%
! TCTCATAATATGACTATGAT+GGG - chr5_3:68396236-68396255 Msa0827240:three_prime_UTR 25.0%
! GATCATAATAAAAAAGGCTA+TGG + chr5_3:68396297-68396316 None:intergenic 25.0%
!!! AGCCTTTTTTATTATGATCA+TGG - chr5_3:68396298-68396317 Msa0827240:three_prime_UTR 25.0%
!! TTTTGAGCACTAACATTTTT+TGG + chr5_3:68396326-68396345 None:intergenic 25.0%
! TATTGAAGAAATCTTGTGTA+AGG + chr5_3:68396499-68396518 None:intergenic 25.0%
! CAAACTGATTATAAGAATTG+AGG + chr5_3:68396592-68396611 None:intergenic 25.0%
! CTCAATTCTTATAATCAGTT+TGG - chr5_3:68396590-68396609 Msa0827240:CDS 25.0%
! ATTCTTATAATCAGTTTGGT+AGG - chr5_3:68396594-68396613 Msa0827240:CDS 25.0%
!!! CGTTAATTTTGTTTAGTTGT+TGG + chr5_3:68396731-68396750 None:intergenic 25.0%
!! TCTCATATTCTCTTTTTACT+AGG - chr5_3:68396830-68396849 Msa0827240:intron 25.0%
!! CTCATATTCTCTTTTTACTA+GGG - chr5_3:68396831-68396850 Msa0827240:intron 25.0%
! TCTCATAATATGACTATGAT+GGG - chr5_3:68396236-68396255 Msa0827240:three_prime_UTR 25.0%
! GATCATAATAAAAAAGGCTA+TGG + chr5_3:68396297-68396316 None:intergenic 25.0%
!!! AGCCTTTTTTATTATGATCA+TGG - chr5_3:68396298-68396317 Msa0827240:three_prime_UTR 25.0%
!! TTTTGAGCACTAACATTTTT+TGG + chr5_3:68396326-68396345 None:intergenic 25.0%
! TATTGAAGAAATCTTGTGTA+AGG + chr5_3:68396499-68396518 None:intergenic 25.0%
! CAAACTGATTATAAGAATTG+AGG + chr5_3:68396592-68396611 None:intergenic 25.0%
! CTCAATTCTTATAATCAGTT+TGG - chr5_3:68396590-68396609 Msa0827240:CDS 25.0%
! ATTCTTATAATCAGTTTGGT+AGG - chr5_3:68396594-68396613 Msa0827240:CDS 25.0%
!!! CGTTAATTTTGTTTAGTTGT+TGG + chr5_3:68396731-68396750 None:intergenic 25.0%
!! TCTCATATTCTCTTTTTACT+AGG - chr5_3:68396830-68396849 Msa0827240:intron 25.0%
!! CTCATATTCTCTTTTTACTA+GGG - chr5_3:68396831-68396850 Msa0827240:intron 25.0%
CTCTCATAATATGACTATGA+TGG - chr5_3:68396235-68396254 Msa0827240:three_prime_UTR 30.0%
CAAATCACTAAGCATATGTA+TGG - chr5_3:68396262-68396281 Msa0827240:three_prime_UTR 30.0%
AATGTTAGTGCTCAAAATTG+TGG - chr5_3:68396329-68396348 Msa0827240:three_prime_UTR 30.0%
ATGTTAGTGCTCAAAATTGT+GGG - chr5_3:68396330-68396349 Msa0827240:three_prime_UTR 30.0%
AAATTTGGTTACACAACTGT+TGG - chr5_3:68396787-68396806 Msa0827240:intron 30.0%
AATTTGGTTACACAACTGTT+GGG - chr5_3:68396788-68396807 Msa0827240:intron 30.0%
TAGTTCCAAGATAGTTGAAT+CGG + chr5_3:68397009-68397028 None:intergenic 30.0%
TATTGGATGAATAATGTGCA+CGG - chr5_3:68397129-68397148 Msa0827240:CDS 30.0%
ACTCAATATTGTAGTGAGTT+GGG - chr5_3:68397255-68397274 Msa0827240:CDS 30.0%
CTCTCATAATATGACTATGA+TGG - chr5_3:68396235-68396254 Msa0827240:three_prime_UTR 30.0%
CAAATCACTAAGCATATGTA+TGG - chr5_3:68396262-68396281 Msa0827240:three_prime_UTR 30.0%
AATGTTAGTGCTCAAAATTG+TGG - chr5_3:68396329-68396348 Msa0827240:three_prime_UTR 30.0%
ATGTTAGTGCTCAAAATTGT+GGG - chr5_3:68396330-68396349 Msa0827240:three_prime_UTR 30.0%
AAATTTGGTTACACAACTGT+TGG - chr5_3:68396787-68396806 Msa0827240:intron 30.0%
AATTTGGTTACACAACTGTT+GGG - chr5_3:68396788-68396807 Msa0827240:intron 30.0%
TAGTTCCAAGATAGTTGAAT+CGG + chr5_3:68397009-68397028 None:intergenic 30.0%
TATTGGATGAATAATGTGCA+CGG - chr5_3:68397129-68397148 Msa0827240:CDS 30.0%
ACTCAATATTGTAGTGAGTT+GGG - chr5_3:68397255-68397274 Msa0827240:CDS 30.0%
TATAATCAGTTTGGTAGGTC+TGG - chr5_3:68396599-68396618 Msa0827240:CDS 35.0%
CTATCTTGGAACTACAACTA+TGG - chr5_3:68397015-68397034 Msa0827240:CDS 35.0%
ATACCATAATGCTGTCTTGA+AGG + chr5_3:68397112-68397131 None:intergenic 35.0%
TCAAGACAGCATTATGGTAT+TGG - chr5_3:68397112-68397131 Msa0827240:CDS 35.0%
CACTCAATATTGTAGTGAGT+TGG - chr5_3:68397254-68397273 Msa0827240:CDS 35.0%
TATAATCAGTTTGGTAGGTC+TGG - chr5_3:68396599-68396618 Msa0827240:CDS 35.0%
CTATCTTGGAACTACAACTA+TGG - chr5_3:68397015-68397034 Msa0827240:CDS 35.0%
ATACCATAATGCTGTCTTGA+AGG + chr5_3:68397112-68397131 None:intergenic 35.0%
TCAAGACAGCATTATGGTAT+TGG - chr5_3:68397112-68397131 Msa0827240:CDS 35.0%
CACTCAATATTGTAGTGAGT+TGG - chr5_3:68397254-68397273 Msa0827240:CDS 35.0%
! GGTAATGGTGATGCATATTG+TGG - chr5_3:68396392-68396411 Msa0827240:CDS 40.0%
! AAACAAGGTCCTTGTTATGC+AGG - chr5_3:68396425-68396444 Msa0827240:CDS 40.0%
TCATTGTTAGGCAAACTTGG+TGG + chr5_3:68396458-68396477 None:intergenic 40.0%
GCATCATTGTTAGGCAAACT+TGG + chr5_3:68396461-68396480 None:intergenic 40.0%
GCCTAACAATGATGCTAATG+TGG - chr5_3:68396466-68396485 Msa0827240:CDS 40.0%
GCCACATTAGCATCATTGTT+AGG + chr5_3:68396470-68396489 None:intergenic 40.0%
GCTCATTTCACACATGAAAC+TGG - chr5_3:68396662-68396681 Msa0827240:CDS 40.0%
TGCTATACCGAAGAGATTGA+TGG - chr5_3:68396907-68396926 Msa0827240:intron 40.0%
! TGATGGACCATCAATCTCTT+CGG + chr5_3:68396917-68396936 None:intergenic 40.0%
! AGAGATTGATGGTCCATCAA+AGG - chr5_3:68396918-68396937 Msa0827240:intron 40.0%
TCATCACAGTAGTCCTTTGA+TGG + chr5_3:68396934-68396953 None:intergenic 40.0%
GTGATGAAAACAACACAGAG+TGG - chr5_3:68396947-68396966 Msa0827240:intron 40.0%
ACCGTAGTAACCTTTGTTAG+GGG + chr5_3:68396980-68396999 None:intergenic 40.0%
GACCGTAGTAACCTTTGTTA+GGG + chr5_3:68396981-68397000 None:intergenic 40.0%
!!! GGAAGAGACAATGGTTTTGA+TGG - chr5_3:68397045-68397064 Msa0827240:CDS 40.0%
ATTGAACTCTCCTGAAACAG+TGG - chr5_3:68397068-68397087 Msa0827240:CDS 40.0%
TGTCCTTCAAGACAGCATTA+TGG - chr5_3:68397106-68397125 Msa0827240:CDS 40.0%
GCAACCATTAGGGCTATTAA+TGG - chr5_3:68397174-68397193 Msa0827240:CDS 40.0%
TCGACCATTAATAGCCCTAA+TGG + chr5_3:68397181-68397200 None:intergenic 40.0%
!! AATGGTCGACTTGAATGTGA+TGG - chr5_3:68397192-68397211 Msa0827240:CDS 40.0%
TAGCAAGTAAGGTTATCACC+AGG + chr5_3:68397288-68397307 None:intergenic 40.0%
! GGTAATGGTGATGCATATTG+TGG - chr5_3:68396392-68396411 Msa0827240:CDS 40.0%
! AAACAAGGTCCTTGTTATGC+AGG - chr5_3:68396425-68396444 Msa0827240:CDS 40.0%
TCATTGTTAGGCAAACTTGG+TGG + chr5_3:68396458-68396477 None:intergenic 40.0%
GCATCATTGTTAGGCAAACT+TGG + chr5_3:68396461-68396480 None:intergenic 40.0%
GCCTAACAATGATGCTAATG+TGG - chr5_3:68396466-68396485 Msa0827240:CDS 40.0%
GCCACATTAGCATCATTGTT+AGG + chr5_3:68396470-68396489 None:intergenic 40.0%
GCTCATTTCACACATGAAAC+TGG - chr5_3:68396662-68396681 Msa0827240:CDS 40.0%
TGCTATACCGAAGAGATTGA+TGG - chr5_3:68396907-68396926 Msa0827240:intron 40.0%
! TGATGGACCATCAATCTCTT+CGG + chr5_3:68396917-68396936 None:intergenic 40.0%
! AGAGATTGATGGTCCATCAA+AGG - chr5_3:68396918-68396937 Msa0827240:intron 40.0%
TCATCACAGTAGTCCTTTGA+TGG + chr5_3:68396934-68396953 None:intergenic 40.0%
GTGATGAAAACAACACAGAG+TGG - chr5_3:68396947-68396966 Msa0827240:intron 40.0%
ACCGTAGTAACCTTTGTTAG+GGG + chr5_3:68396980-68396999 None:intergenic 40.0%
GACCGTAGTAACCTTTGTTA+GGG + chr5_3:68396981-68397000 None:intergenic 40.0%
!!! GGAAGAGACAATGGTTTTGA+TGG - chr5_3:68397045-68397064 Msa0827240:CDS 40.0%
ATTGAACTCTCCTGAAACAG+TGG - chr5_3:68397068-68397087 Msa0827240:CDS 40.0%
TGTCCTTCAAGACAGCATTA+TGG - chr5_3:68397106-68397125 Msa0827240:CDS 40.0%
GCAACCATTAGGGCTATTAA+TGG - chr5_3:68397174-68397193 Msa0827240:CDS 40.0%
TCGACCATTAATAGCCCTAA+TGG + chr5_3:68397181-68397200 None:intergenic 40.0%
!! AATGGTCGACTTGAATGTGA+TGG - chr5_3:68397192-68397211 Msa0827240:CDS 40.0%
TAGCAAGTAAGGTTATCACC+AGG + chr5_3:68397288-68397307 None:intergenic 40.0%
CAAAATTGTGGGTGTGCTGA+AGG - chr5_3:68396341-68396360 Msa0827240:exon 45.0%
CATTACCGCAATACCCGTAT+TGG + chr5_3:68396379-68396398 None:intergenic 45.0%
! CAATACGGGTATTGCGGTAA+TGG - chr5_3:68396377-68396396 Msa0827240:CDS 45.0%
!! TAGGCAAACTTGGTGGTGTT+TGG + chr5_3:68396451-68396470 None:intergenic 45.0%
!! GTTTGGTAGGTCTGGTTCTT+TGG - chr5_3:68396607-68396626 Msa0827240:CDS 45.0%
TTTGGATGACTCCAAACGTG+AGG - chr5_3:68396625-68396644 Msa0827240:CDS 45.0%
! TAACCTTTGTTAGGGGCACA+TGG + chr5_3:68396973-68396992 None:intergenic 45.0%
GCCCCTAACAAAGGTTACTA+CGG - chr5_3:68396976-68396995 Msa0827240:CDS 45.0%
CGACCGTAGTAACCTTTGTT+AGG + chr5_3:68396982-68397001 None:intergenic 45.0%
AACAAAGGTTACTACGGTCG+TGG - chr5_3:68396982-68397001 Msa0827240:CDS 45.0%
! GTGGACCGATTCAACTATCT+TGG - chr5_3:68397001-68397020 Msa0827240:CDS 45.0%
AACTACAACTATGGACCAGC+TGG - chr5_3:68397024-68397043 Msa0827240:CDS 45.0%
AAACCATTGTCTCTTCCAGC+TGG + chr5_3:68397042-68397061 None:intergenic 45.0%
GAAGGACACAACAGCATCAT+TGG + chr5_3:68397094-68397113 None:intergenic 45.0%
TAATGGTTGCACCAAAGCCT+TGG + chr5_3:68397164-68397183 None:intergenic 45.0%
! AAGGCTTTGGTGCAACCATT+AGG - chr5_3:68397163-68397182 Msa0827240:CDS 45.0%
! AGGCTTTGGTGCAACCATTA+GGG - chr5_3:68397164-68397183 Msa0827240:CDS 45.0%
! GCTAGCTAGCTTAGCAAGTA+AGG + chr5_3:68397299-68397318 None:intergenic 45.0%
CAAAATTGTGGGTGTGCTGA+AGG - chr5_3:68396341-68396360 Msa0827240:exon 45.0%
CATTACCGCAATACCCGTAT+TGG + chr5_3:68396379-68396398 None:intergenic 45.0%
! CAATACGGGTATTGCGGTAA+TGG - chr5_3:68396377-68396396 Msa0827240:CDS 45.0%
!! TAGGCAAACTTGGTGGTGTT+TGG + chr5_3:68396451-68396470 None:intergenic 45.0%
!! GTTTGGTAGGTCTGGTTCTT+TGG - chr5_3:68396607-68396626 Msa0827240:CDS 45.0%
TTTGGATGACTCCAAACGTG+AGG - chr5_3:68396625-68396644 Msa0827240:CDS 45.0%
! TAACCTTTGTTAGGGGCACA+TGG + chr5_3:68396973-68396992 None:intergenic 45.0%
GCCCCTAACAAAGGTTACTA+CGG - chr5_3:68396976-68396995 Msa0827240:CDS 45.0%
CGACCGTAGTAACCTTTGTT+AGG + chr5_3:68396982-68397001 None:intergenic 45.0%
AACAAAGGTTACTACGGTCG+TGG - chr5_3:68396982-68397001 Msa0827240:CDS 45.0%
! GTGGACCGATTCAACTATCT+TGG - chr5_3:68397001-68397020 Msa0827240:CDS 45.0%
AACTACAACTATGGACCAGC+TGG - chr5_3:68397024-68397043 Msa0827240:CDS 45.0%
AAACCATTGTCTCTTCCAGC+TGG + chr5_3:68397042-68397061 None:intergenic 45.0%
GAAGGACACAACAGCATCAT+TGG + chr5_3:68397094-68397113 None:intergenic 45.0%
TAATGGTTGCACCAAAGCCT+TGG + chr5_3:68397164-68397183 None:intergenic 45.0%
! AAGGCTTTGGTGCAACCATT+AGG - chr5_3:68397163-68397182 Msa0827240:CDS 45.0%
! AGGCTTTGGTGCAACCATTA+GGG - chr5_3:68397164-68397183 Msa0827240:CDS 45.0%
! GCTAGCTAGCTTAGCAAGTA+AGG + chr5_3:68397299-68397318 None:intergenic 45.0%
GGAGTGTGTTGCAGCCAATA+CGG - chr5_3:68396362-68396381 Msa0827240:CDS 50.0%
GAGTGTGTTGCAGCCAATAC+GGG - chr5_3:68396363-68396382 Msa0827240:CDS 50.0%
! TGCAGCCAATACGGGTATTG+CGG - chr5_3:68396371-68396390 Msa0827240:CDS 50.0%
GGTGATGCATATTGTGGCAC+AGG - chr5_3:68396398-68396417 Msa0827240:CDS 50.0%
TGTGGCACAGGATGCAAACA+AGG - chr5_3:68396410-68396429 Msa0827240:CDS 50.0%
GGTGTTTGGCCTGCATAACA+AGG + chr5_3:68396437-68396456 None:intergenic 50.0%
! CAAGCTGATTCTAGCTGTGC+AGG - chr5_3:68396530-68396549 Msa0827240:CDS 50.0%
GCATCATTGGCCACTGTTTC+AGG + chr5_3:68397081-68397100 None:intergenic 50.0%
! GGTGTCATCAACCAAGGCTT+TGG - chr5_3:68397150-68397169 Msa0827240:CDS 50.0%
CACGAGTCTGAACTGTGGTT+GGG + chr5_3:68397224-68397243 None:intergenic 50.0%
ACACGAGTCTGAACTGTGGT+TGG + chr5_3:68397225-68397244 None:intergenic 50.0%
ACCACAGTTCAGACTCGTGT+TGG - chr5_3:68397225-68397244 Msa0827240:CDS 50.0%
ACCAACACGAGTCTGAACTG+TGG + chr5_3:68397229-68397248 None:intergenic 50.0%
GGAGTGTGTTGCAGCCAATA+CGG - chr5_3:68396362-68396381 Msa0827240:CDS 50.0%
GAGTGTGTTGCAGCCAATAC+GGG - chr5_3:68396363-68396382 Msa0827240:CDS 50.0%
! TGCAGCCAATACGGGTATTG+CGG - chr5_3:68396371-68396390 Msa0827240:CDS 50.0%
GGTGATGCATATTGTGGCAC+AGG - chr5_3:68396398-68396417 Msa0827240:CDS 50.0%
TGTGGCACAGGATGCAAACA+AGG - chr5_3:68396410-68396429 Msa0827240:CDS 50.0%
GGTGTTTGGCCTGCATAACA+AGG + chr5_3:68396437-68396456 None:intergenic 50.0%
! CAAGCTGATTCTAGCTGTGC+AGG - chr5_3:68396530-68396549 Msa0827240:CDS 50.0%
GCATCATTGGCCACTGTTTC+AGG + chr5_3:68397081-68397100 None:intergenic 50.0%
! GGTGTCATCAACCAAGGCTT+TGG - chr5_3:68397150-68397169 Msa0827240:CDS 50.0%
CACGAGTCTGAACTGTGGTT+GGG + chr5_3:68397224-68397243 None:intergenic 50.0%
ACACGAGTCTGAACTGTGGT+TGG + chr5_3:68397225-68397244 None:intergenic 50.0%
ACCACAGTTCAGACTCGTGT+TGG - chr5_3:68397225-68397244 Msa0827240:CDS 50.0%
ACCAACACGAGTCTGAACTG+TGG + chr5_3:68397229-68397248 None:intergenic 50.0%
AGCAGCTGCAACCTCACGTT+TGG + chr5_3:68396639-68396658 None:intergenic 55.0%
TGGCCATGTGCCCCTAACAA+AGG - chr5_3:68396967-68396986 Msa0827240:intron 55.0%
GGACCAGCTGGAAGAGACAA+TGG - chr5_3:68397036-68397055 Msa0827240:CDS 55.0%
!! GTGCACGGTGTCATCAACCA+AGG - chr5_3:68397144-68397163 Msa0827240:CDS 55.0%
!! AGTGAGTTGGGTGTTGCTCC+TGG - chr5_3:68397267-68397286 Msa0827240:CDS 55.0%
AGCAGCTGCAACCTCACGTT+TGG + chr5_3:68396639-68396658 None:intergenic 55.0%
TGGCCATGTGCCCCTAACAA+AGG - chr5_3:68396967-68396986 Msa0827240:intron 55.0%
GGACCAGCTGGAAGAGACAA+TGG - chr5_3:68397036-68397055 Msa0827240:CDS 55.0%
!! GTGCACGGTGTCATCAACCA+AGG - chr5_3:68397144-68397163 Msa0827240:CDS 55.0%
!! AGTGAGTTGGGTGTTGCTCC+TGG - chr5_3:68397267-68397286 Msa0827240:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 68396211 68397451 68396211 ID=Msa0827240;Name=Msa0827240
chr5_3 mRNA 68396211 68397451 68396211 ID=Msa0827240-mRNA-1;Parent=Msa0827240;Name=Msa0827240-mRNA-1;_AED=0.00;_eAED=0.00;_QI=34|1|1|1|1|1|2|143|282
chr5_3 exon 68396211 68396760 68396211 ID=Msa0827240-mRNA-1:exon:21496;Parent=Msa0827240-mRNA-1
chr5_3 exon 68396976 68397451 68396976 ID=Msa0827240-mRNA-1:exon:21495;Parent=Msa0827240-mRNA-1
chr5_3 five_prime_UTR 68397418 68397451 68397418 ID=Msa0827240-mRNA-1:five_prime_utr;Parent=Msa0827240-mRNA-1
chr5_3 CDS 68396976 68397417 68396976 ID=Msa0827240-mRNA-1:cds;Parent=Msa0827240-mRNA-1
chr5_3 CDS 68396354 68396760 68396354 ID=Msa0827240-mRNA-1:cds;Parent=Msa0827240-mRNA-1
chr5_3 three_prime_UTR 68396211 68396353 68396211 ID=Msa0827240-mRNA-1:three_prime_utr;Parent=Msa0827240-mRNA-1
Gene Sequence

>Msa0827240

ATGACTATGATGGGAAACAAATCACTAAGCATATGTATGGCAACACTTGCCATAGCCTTTTTTATTATGATCATGGTTCCAAAAAATGTTAGTGCTCAAAATTGTGGGTGTGCTGAAGGAGTGTGTTGCAGCCAATACGGGTATTGCGGTAATGGTGATGCATATTGTGGCACAGGATGCAAACAAGGTCCTTGTTATGCAGGCCAAACACCACCAAGTTTGCCTAACAATGATGCTAATGTGGCTGACATCCTTACACAAGATTTCTTCAATAGTATAATTGATCAAGCTGATTCTAGCTGTGCAGGAAAGAACTTCTACACAAGAGCTGCTTTTCTTGATGCCCTCAATTCTTATAATCAGTTTGGTAGGTCTGGTTCTTTGGATGACTCCAAACGTGAGGTTGCAGCTGCTTTTGCTCATTTCACACATGAAACTGGACATTTTTGCTATACCGAAGAGATTGATGGTCCATCAAAGGACTACTGTGATGAAAACAACACAGAGTGGCCATGTGCCCCTAACAAAGGTTACTACGGTCGTGGACCGATTCAACTATCTTGGAACTACAACTATGGACCAGCTGGAAGAGACAATGGTTTTGATGGATTGAACTCTCCTGAAACAGTGGCCAATGATGCTGTTGTGTCCTTCAAGACAGCATTATGGTATTGGATGAATAATGTGCACGGTGTCATCAACCAAGGCTTTGGTGCAACCATTAGGGCTATTAATGGTCGACTTGAATGTGATGGTGCTAACCCAACCACAGTTCAGACTCGTGTTGGTTACTACACTCAATATTGTAGTGAGTTGGGTGTTGCTCCTGGTGATAACCTTACTTGCTAA
Protein sequence

>Msa0827240

MTMMGNKSLSICMATLAIAFFIMIMVPKNVSAQNCGCAEGVCCSQYGYCGNGDAYCGTGCKQGPCYAGQTPPSLPNNDANVADILTQDFFNSIIDQADSSCAGKNFYTRAAFLDALNSYNQFGRSGSLDDSKREVAAAFAHFTHETGHFCYTEEIDGPSKDYCDENNTEWPCAPNKGYYGRGPIQLSWNYNYGPAGRDNGFDGLNSPETVANDAVVSFKTALWYWMNNVHGVINQGFGATIRAINGRLECDGANPTTVQTRVGYYTQYCSELGVAPGDNLTC*