AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0304940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0304940 MtrunA17_Chr2g0326101 49.388 245 81 8 1 214 232 464 1.51e-55 183
Msa0304940 MtrunA17_Chr2g0326101 46.122 245 78 7 1 208 1 228 1.46e-47 162
Msa0304940 MtrunA17_Chr2g0319901 61.074 149 44 2 85 219 80 228 1.65e-55 176
Msa0304940 MtrunA17_Chr2g0333201 49.798 247 84 8 1 219 3 237 1.93e-55 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 79 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCTGAGTAAAGATATTT+AGG 0.147252 2_3:-62314664 Msa0304940:exon
GCCATGACAGATCCCATATT+TGG 0.209504 2_3:+62314721 None:intergenic
ATGTTCCTTGTCAGCATTCA+TGG 0.213484 2_3:+62316985 None:intergenic
TTAAATGAATTCTATTTATT+AGG 0.246763 2_3:+62314423 None:intergenic
CCCAATTAAACGAGCCAAAA+AGG 0.289069 2_3:+62314454 None:intergenic
TTAAGCCCGAGAAATAATTA+AGG 0.291848 2_3:+62314481 None:intergenic
ACAGTTGAGGAAGCCAAATA+TGG 0.300778 2_3:-62314734 Msa0304940:CDS
TGAGTTGTATGTTAACTCTT+AGG 0.314820 2_3:-62314565 Msa0304940:three_prime_UTR
TCAGAAAGGTTAAGAGAATC+AGG 0.328389 2_3:-62316922 Msa0304940:intron
TATTGCTAAAGTGGCAGATT+TGG 0.335525 2_3:+62317111 None:intergenic
AGCCATTCCGGATTTCAAAA+AGG 0.338321 2_3:-62317158 Msa0304940:CDS
CACCGTCCTGTCCTGCGGTT+TGG 0.338544 2_3:-62318192 Msa0304940:five_prime_UTR
GTTACTCGGCAACTAGTTAA+TGG 0.344977 2_3:-62317034 Msa0304940:CDS
CGGAGAAGCTATAGCCATTC+CGG 0.350939 2_3:-62317170 Msa0304940:intron
GCTGCAGGAGGCGATGGATA+TGG 0.362176 2_3:+62317691 None:intergenic
ATCTGCCACTTTAGCAATAC+AGG 0.375351 2_3:-62317107 Msa0304940:CDS
TCATGGCTTGAAAGGTGATA+TGG 0.376146 2_3:+62317002 None:intergenic
TCTTTCACGTCCATGATAAC+TGG 0.380417 2_3:+62314793 None:intergenic
CTAAAGTGGCAGATTTGGTT+AGG 0.389297 2_3:+62317116 None:intergenic
TAGATCCCTTAATTATTTCT+CGG 0.401123 2_3:-62314487 Msa0304940:three_prime_UTR
TGCCTGAGTAAAGATATTTA+GGG 0.407035 2_3:-62314663 Msa0304940:exon
CCATCTCGATATGGACGAAT+TGG 0.412531 2_3:+62317056 None:intergenic
GGTGGTGACCTTCGAGCATA+TGG 0.427486 2_3:+62317973 None:intergenic
ATGGTTGACTCAGTTCAAAT+TGG 0.433101 2_3:-62314389 Msa0304940:three_prime_UTR
ATAATCCTGTATTGCTAAAG+TGG 0.435729 2_3:+62317102 None:intergenic
TGCAGCTAGATTAGTCAAGC+TGG 0.441470 2_3:-62317674 Msa0304940:CDS
TTTCTGCGAATTGGGGCTTA+GGG 0.442584 2_3:-62318127 Msa0304940:five_prime_UTR
GCCGAGTAACCATCTCGATA+TGG 0.446899 2_3:+62317047 None:intergenic
ACTGTCGAGCGGTATTCATA+AGG 0.452038 2_3:+62314752 None:intergenic
AGATCCCTTAATTATTTCTC+GGG 0.456806 2_3:-62314486 Msa0304940:three_prime_UTR
GATGAAGGCGCTGAAGCTGC+TGG 0.460674 2_3:-62314691 Msa0304940:CDS
TGAGAAATCGAGTGTCGAAA+TGG 0.464953 2_3:-62318100 Msa0304940:exon
GAGGAACCAAACCGCAGGAC+AGG 0.465131 2_3:+62318186 None:intergenic
TTTGGTTAGGAGAAGAAACT+TGG 0.474276 2_3:+62317129 None:intergenic
TTCTGCTACTCCTACCTGCT+CGG 0.483294 2_3:-62318019 Msa0304940:CDS
TGGTGGTGAAATCAGTTAAG+AGG 0.489867 2_3:-62314634 Msa0304940:three_prime_UTR
CTGTCGAGCGGTATTCATAA+GGG 0.496417 2_3:+62314753 None:intergenic
GGATGATGATCCAGTTATCA+TGG 0.510725 2_3:-62314803 Msa0304940:CDS
TAAGCCCGAGAAATAATTAA+GGG 0.512273 2_3:+62314482 None:intergenic
CTTGAAAGGTGATATGGTAA+AGG 0.513491 2_3:+62317008 None:intergenic
AATCAGTTAAGAGGTTGTCT+TGG 0.524040 2_3:-62314625 Msa0304940:three_prime_UTR
AATCTAGCTGCAGGAGGCGA+TGG 0.532648 2_3:+62317685 None:intergenic
GCTGGCTTTCACCTCCGAGC+AGG 0.533675 2_3:+62318005 None:intergenic
CAGTTGAGGAAGCCAAATAT+GGG 0.536691 2_3:-62314733 Msa0304940:CDS
AGCATATGGACGATATCTGC+TGG 0.543046 2_3:+62317987 None:intergenic
GCAGCTAGATTAGTCAAGCT+GGG 0.544326 2_3:-62317673 Msa0304940:intron
GTCAGCATTCATGGCTTGAA+AGG 0.544835 2_3:+62316994 None:intergenic
AGCTTGACTAATCTAGCTGC+AGG 0.550250 2_3:+62317676 None:intergenic
CAAGGGCACTCTCATGCAGC+TGG 0.551659 2_3:-62315587 Msa0304940:intron
GTTATCATGGACGTGAAAGA+TGG 0.553405 2_3:-62314790 Msa0304940:CDS
AATACCTTTGCTTTGACGAG+AGG 0.555040 2_3:+62318167 None:intergenic
GGATGAAAGAGAGCAGTACT+TGG 0.557944 2_3:-62316901 Msa0304940:intron
TGAATACCGCTCGACAGTTG+AGG 0.564621 2_3:-62314747 Msa0304940:CDS
GTAGCAGAAGACGAAGAAGA+AGG 0.565840 2_3:+62318033 None:intergenic
GCCAAATATGGGATCTGTCA+TGG 0.570040 2_3:-62314722 Msa0304940:CDS
TTCTTCACCGTCCTGTCCTG+CGG 0.571363 2_3:-62318197 Msa0304940:five_prime_UTR
GTCATGGCAAAACAAGATGA+AGG 0.571769 2_3:-62314706 Msa0304940:CDS
GTTAGGAGAAGAAACTTGGA+AGG 0.575655 2_3:+62317133 None:intergenic
GATAAACGCGGTAGATAATG+AGG 0.581551 2_3:+62318271 None:intergenic
GGTTCCTCTCGTCAAAGCAA+AGG 0.590634 2_3:-62318171 Msa0304940:five_prime_UTR
TCAAGCCATGAATGCTGACA+AGG 0.596210 2_3:-62316990 Msa0304940:CDS
TCCATATCGAGATGGTTACT+CGG 0.600828 2_3:-62317048 Msa0304940:CDS
CCAATTCGTCCATATCGAGA+TGG 0.612639 2_3:-62317056 Msa0304940:CDS
GCCTGAGTAAAGATATTTAG+GGG 0.617144 2_3:-62314662 Msa0304940:exon
AGGACGGTGAAGAACGAACG+AGG 0.618237 2_3:+62318206 None:intergenic
GACGAGAGGAACCAAACCGC+AGG 0.619819 2_3:+62318181 None:intergenic
GTTAACAATAAAGTCGCGGT+AGG 0.622455 2_3:+62317732 None:intergenic
TATGCTCGAAGGTCACCACC+TGG 0.625018 2_3:-62317970 Msa0304940:intron
AACCAAACCGCAGGACAGGA+CGG 0.629740 2_3:+62318190 None:intergenic
TGGCTTCCTCAACTGTCGAG+CGG 0.636593 2_3:+62314741 None:intergenic
CCTGAGTAAAGATATTTAGG+GGG 0.643099 2_3:-62314661 Msa0304940:exon
GCTTTCACCTCCGAGCAGGT+AGG 0.649215 2_3:+62318009 None:intergenic
CTGAGTAAAGATATTTAGGG+GGG 0.652095 2_3:-62314660 Msa0304940:exon
GATATCGTCCATATGCTCGA+AGG 0.658364 2_3:-62317981 Msa0304940:CDS
GGAGCGATAGAAAACGAACA+AGG 0.668006 2_3:+62318228 None:intergenic
TGCTACTCCTACCTGCTCGG+AGG 0.671023 2_3:-62318016 Msa0304940:CDS
TTGACTAATCTAGCTGCAGG+AGG 0.674170 2_3:+62317679 None:intergenic
TAACGTTAACAATAAAGTCG+CGG 0.689934 2_3:+62317728 None:intergenic
GGACGGTGAAGAACGAACGA+GGG 0.707025 2_3:+62318207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATGAATTCTATTTATT+AGG + chr2_3:62318238-62318257 None:intergenic 10.0%
!!! ATATTGTTTGTTATTGTTAA+TGG - chr2_3:62314760-62314779 Msa0304940:CDS 15.0%
!! TGAAAAGATCATAAAATAAT+GGG + chr2_3:62316003-62316022 None:intergenic 15.0%
!! ATGAAAAGATCATAAAATAA+TGG + chr2_3:62316004-62316023 None:intergenic 15.0%
!!! TGTTGATAATGTTTTTAAAT+AGG + chr2_3:62316048-62316067 None:intergenic 15.0%
!! TAAAAAAAAAACACATTACA+GGG + chr2_3:62316111-62316130 None:intergenic 15.0%
!! TTAAAAAAAAAACACATTAC+AGG + chr2_3:62316112-62316131 None:intergenic 15.0%
!! GTGTTTGTATATATTAAAAA+CGG - chr2_3:62316144-62316163 Msa0304940:intron 15.0%
!! TATTTCTTTATATATAGACA+AGG + chr2_3:62316238-62316257 None:intergenic 15.0%
!!! TTATTAATCTTTTCAACAAT+GGG - chr2_3:62316644-62316663 Msa0304940:intron 15.0%
!!! ATATAATTCTTTGTTTTAGA+AGG - chr2_3:62316752-62316771 Msa0304940:intron 15.0%
!!! AAGCAAATAATATTTTTGTT+AGG + chr2_3:62316892-62316911 None:intergenic 15.0%
!! ATCTAATAATTGTAAGAAAA+AGG + chr2_3:62317118-62317137 None:intergenic 15.0%
!! ATTAAAGTAATGATATTGTT+AGG - chr2_3:62317649-62317668 Msa0304940:intron 15.0%
!! TTAAAGTAATGATATTGTTA+GGG - chr2_3:62317650-62317669 Msa0304940:intron 15.0%
!!! GTTGATTTTTTTTTTAATTC+AGG - chr2_3:62317831-62317850 Msa0304940:intron 15.0%
!! TCATTTAATGTTAAAAAGAA+TGG - chr2_3:62318250-62318269 Msa0304940:five_prime_UTR 15.0%
!!! TAGGATTTTCTAATTTGATT+CGG + chr2_3:62314910-62314929 None:intergenic 20.0%
!! AACATGATAATAATTGCAAT+AGG + chr2_3:62315040-62315059 None:intergenic 20.0%
!! GGATTATCTATGTATTTATT+TGG + chr2_3:62315200-62315219 None:intergenic 20.0%
!! ATTTCTCATAAGAAAAATCT+AGG - chr2_3:62315272-62315291 Msa0304940:intron 20.0%
!! TGTATGCAAAGAAAAATATT+GGG + chr2_3:62315850-62315869 None:intergenic 20.0%
!! ATGTATGCAAAGAAAAATAT+TGG + chr2_3:62315851-62315870 None:intergenic 20.0%
!! AAAAAAAAAACACATTACAG+GGG + chr2_3:62316110-62316129 None:intergenic 20.0%
!! AATATATACAAACACATTAC+AGG + chr2_3:62316139-62316158 None:intergenic 20.0%
!!! TAGTTTTTCAAGAGAAAAAA+GGG + chr2_3:62316212-62316231 None:intergenic 20.0%
!!! CTTATTAATCTTTTCAACAA+TGG - chr2_3:62316643-62316662 Msa0304940:intron 20.0%
!! ATGTATCTAGTCTATAATAT+TGG + chr2_3:62317270-62317289 None:intergenic 20.0%
!!! ATCAACTTTTTCATTTTTTG+AGG - chr2_3:62317397-62317416 Msa0304940:intron 20.0%
!!! TCAACTTTTTCATTTTTTGA+GGG - chr2_3:62317398-62317417 Msa0304940:intron 20.0%
!! TGAAAAAGAAACACTAAAAT+TGG + chr2_3:62317491-62317510 None:intergenic 20.0%
!! TAAAGTAATGATATTGTTAG+GGG - chr2_3:62317651-62317670 Msa0304940:intron 20.0%
!! ATAAAATGAGCATCAATATT+AGG - chr2_3:62317745-62317764 Msa0304940:CDS 20.0%
!!! GATTTTTTTTTTAATTCAGG+AGG - chr2_3:62317834-62317853 Msa0304940:intron 20.0%
!!! ATTTTTTGAAGTAAGTCTGA+GGG + chr2_3:62314595-62314614 None:intergenic 25.0%
!!! TGTTTGTTATTGTTAATGGA+TGG - chr2_3:62314764-62314783 Msa0304940:CDS 25.0%
! AAAAGTAGAGAAACAAACAA+TGG + chr2_3:62314878-62314897 None:intergenic 25.0%
! TATGCATAACATAAGCTATA+AGG + chr2_3:62315156-62315175 None:intergenic 25.0%
!! TAAGAGCAAAATTTTCAGAA+AGG - chr2_3:62315722-62315741 Msa0304940:intron 25.0%
! TACAATGTAAGCAAATTAGT+GGG + chr2_3:62315939-62315958 None:intergenic 25.0%
! TTACAATGTAAGCAAATTAG+TGG + chr2_3:62315940-62315959 None:intergenic 25.0%
!!! TTTAGCTTTTGCTTTTTTCT+TGG - chr2_3:62315961-62315980 Msa0304940:intron 25.0%
!! ATAAAATAATGGGAACGAAA+GGG + chr2_3:62315993-62316012 None:intergenic 25.0%
!!! CTAGTTTTTCAAGAGAAAAA+AGG + chr2_3:62316213-62316232 None:intergenic 25.0%
! ACTTACCTACAACAATTAAA+CGG + chr2_3:62316544-62316563 None:intergenic 25.0%
!! TAAGCAAAAAACAACGTTTT+AGG - chr2_3:62317219-62317238 Msa0304940:intron 25.0%
! ATCTAGTCTATAATATTGGT+TGG + chr2_3:62317266-62317285 None:intergenic 25.0%
!!! GTTGCTAGAATTCATTTTTT+AGG + chr2_3:62317308-62317327 None:intergenic 25.0%
!! ATTGGTAACAGAAAATTTAG+AGG + chr2_3:62317473-62317492 None:intergenic 25.0%
!! TGTGTTTTATGTTAAAAACG+TGG - chr2_3:62318059-62318078 Msa0304940:CDS 25.0%
!! GTGTTTTATGTTAAAAACGT+GGG - chr2_3:62318060-62318079 Msa0304940:CDS 25.0%
!! TAGATCCCTTAATTATTTCT+CGG - chr2_3:62318171-62318190 Msa0304940:five_prime_UTR 25.0%
AAAAATCAACTCGATAAACG+CGG + chr2_3:62314402-62314421 None:intergenic 30.0%
!! TACACTATTTTTCTGCGAAT+TGG - chr2_3:62314522-62314541 Msa0304940:three_prime_UTR 30.0%
!! ACACTATTTTTCTGCGAATT+GGG - chr2_3:62314523-62314542 Msa0304940:three_prime_UTR 30.0%
!!! CATTTTTTGAAGTAAGTCTG+AGG + chr2_3:62314596-62314615 None:intergenic 30.0%
GGATGGTTGAATGAATTTAA+TGG - chr2_3:62314781-62314800 Msa0304940:CDS 30.0%
AGCTAGCTAATTCTCTATAA+TGG + chr2_3:62314823-62314842 None:intergenic 30.0%
TAACGTTAACAATAAAGTCG+CGG + chr2_3:62314933-62314952 None:intergenic 30.0%
AAAGGTTTATGATTGTGAAC+AGG + chr2_3:62315416-62315435 None:intergenic 30.0%
!!! AAACCTTTTTTCTTCTTCAC+AGG - chr2_3:62315428-62315447 Msa0304940:intron 30.0%
AAACCTGTGAAGAAGAAAAA+AGG + chr2_3:62315434-62315453 None:intergenic 30.0%
ATAATCCTGTATTGCTAAAG+TGG + chr2_3:62315559-62315578 None:intergenic 30.0%
TTTCTTTGCATACATAGCAA+TGG - chr2_3:62315856-62315875 Msa0304940:intron 30.0%
! CATAAAATAATGGGAACGAA+AGG + chr2_3:62315994-62316013 None:intergenic 30.0%
AGTTAATATCCCCTTTATCA+CGG - chr2_3:62316170-62316189 Msa0304940:intron 30.0%
TCAAGAGAAAAAAGGGAATA+GGG + chr2_3:62316205-62316224 None:intergenic 30.0%
TTCAAGAGAAAAAAGGGAAT+AGG + chr2_3:62316206-62316225 None:intergenic 30.0%
CTTTGATCAAAACGACTAAT+AGG + chr2_3:62316274-62316293 None:intergenic 30.0%
AATTGTTGTAGGTAAGTTGT+TGG - chr2_3:62316547-62316566 Msa0304940:intron 30.0%
ATTGTTGTAGGTAAGTTGTT+GGG - chr2_3:62316548-62316567 Msa0304940:intron 30.0%
ATGAAATGTAAAGTAGCTGA+GGG + chr2_3:62316802-62316821 None:intergenic 30.0%
!!! AGTTTTTTGTCCAACGAAAA+GGG - chr2_3:62316836-62316855 Msa0304940:intron 30.0%
CTCTTGAAGAATAAAGGTAA+AGG + chr2_3:62316955-62316974 None:intergenic 30.0%
AGAAGACTCTTGAAGAATAA+AGG + chr2_3:62316961-62316980 None:intergenic 30.0%
ATTTAGCGTATCTACTCATT+TGG - chr2_3:62316995-62317014 Msa0304940:CDS 30.0%
GGTTCTTGTTCACATAATTA+CGG - chr2_3:62317016-62317035 Msa0304940:CDS 30.0%
GTTCTTGTTCACATAATTAC+GGG - chr2_3:62317017-62317036 Msa0304940:CDS 30.0%
!!! TTTGTTTTGTTCAGGTACAA+GGG - chr2_3:62317054-62317073 Msa0304940:CDS 30.0%
TGCTTATAATAGTGACTGAA+GGG + chr2_3:62317205-62317224 None:intergenic 30.0%
TTGCTTATAATAGTGACTGA+AGG + chr2_3:62317206-62317225 None:intergenic 30.0%
TGGTTGGATACATCATTTAT+TGG + chr2_3:62317250-62317269 None:intergenic 30.0%
!! ATTGTTAGGGGAATTTTTAG+AGG - chr2_3:62317663-62317682 Msa0304940:intron 30.0%
!! TTGTTAGGGGAATTTTTAGA+GGG - chr2_3:62317664-62317683 Msa0304940:intron 30.0%
TTGCCTGAGTAAAGATATTT+AGG - chr2_3:62317994-62318013 Msa0304940:CDS 30.0%
TGCCTGAGTAAAGATATTTA+GGG - chr2_3:62317995-62318014 Msa0304940:CDS 30.0%
TGAGTTGTATGTTAACTCTT+AGG - chr2_3:62318093-62318112 Msa0304940:exon 30.0%
! AGATCCCTTAATTATTTCTC+GGG - chr2_3:62318172-62318191 Msa0304940:five_prime_UTR 30.0%
TAAGCCCGAGAAATAATTAA+GGG + chr2_3:62318179-62318198 None:intergenic 30.0%
TTAAGCCCGAGAAATAATTA+AGG + chr2_3:62318180-62318199 None:intergenic 30.0%
!! CACTATTTTTCTGCGAATTG+GGG - chr2_3:62314524-62314543 Msa0304940:three_prime_UTR 35.0%
TTAAACAAAACTCACTCACC+AGG + chr2_3:62314709-62314728 None:intergenic 35.0%
AATTTCGTGCTTGAATCTGT+CGG + chr2_3:62315089-62315108 None:intergenic 35.0%
TGTCATCGAATATGCACAAA+TGG + chr2_3:62315367-62315386 None:intergenic 35.0%
!!! ATCCTTTTTGAAATCCGGAA+TGG + chr2_3:62315505-62315524 None:intergenic 35.0%
!!! GAAGGATCCTTTTTGAAATC+CGG + chr2_3:62315510-62315529 None:intergenic 35.0%
TTTGGTTAGGAGAAGAAACT+TGG + chr2_3:62315532-62315551 None:intergenic 35.0%
TATTGCTAAAGTGGCAGATT+TGG + chr2_3:62315550-62315569 None:intergenic 35.0%
CTTGAAAGGTGATATGGTAA+AGG + chr2_3:62315653-62315672 None:intergenic 35.0%
! TTTGCTCTTATTAAGCACTG+TGG + chr2_3:62315712-62315731 None:intergenic 35.0%
TCAGAAAGGTTAAGAGAATC+AGG - chr2_3:62315736-62315755 Msa0304940:intron 35.0%
! GGTTTCTTTGCATGACATTT+TGG - chr2_3:62315877-62315896 Msa0304940:intron 35.0%
ATGTAAGCAAATTAGTGGGA+GGG + chr2_3:62315935-62315954 None:intergenic 35.0%
AATGTAAGCAAATTAGTGGG+AGG + chr2_3:62315936-62315955 None:intergenic 35.0%
TCATCTGTTCGTATGCAATT+TGG - chr2_3:62316020-62316039 Msa0304940:intron 35.0%
CATCTGTTCGTATGCAATTT+GGG - chr2_3:62316021-62316040 Msa0304940:intron 35.0%
GTTAATATCCCCTTTATCAC+GGG - chr2_3:62316171-62316190 Msa0304940:intron 35.0%
TTAATATCCCCTTTATCACG+GGG - chr2_3:62316172-62316191 Msa0304940:intron 35.0%
GGTTAAATGCATAAATTGCG+TGG + chr2_3:62316311-62316330 None:intergenic 35.0%
ATGGACCGTTTAATTGTTGT+AGG - chr2_3:62316536-62316555 Msa0304940:intron 35.0%
AGTTGTTGGGAAATGAATGA+TGG - chr2_3:62316561-62316580 Msa0304940:intron 35.0%
GTTGTTGGGAAATGAATGAT+GGG - chr2_3:62316562-62316581 Msa0304940:intron 35.0%
TTGTTGGGAAATGAATGATG+GGG - chr2_3:62316563-62316582 Msa0304940:intron 35.0%
! ATCTTTTCAACAATGGGTGA+TGG - chr2_3:62316650-62316669 Msa0304940:intron 35.0%
! TCTTTTCAACAATGGGTGAT+GGG - chr2_3:62316651-62316670 Msa0304940:intron 35.0%
! TGTTCGTCTTGTTCTTCAAT+GGG + chr2_3:62316704-62316723 None:intergenic 35.0%
CATGAAATGTAAAGTAGCTG+AGG + chr2_3:62316803-62316822 None:intergenic 35.0%
!!! GAGTTTTTTGTCCAACGAAA+AGG - chr2_3:62316835-62316854 Msa0304940:intron 35.0%
!!! GTTTTTTGTCCAACGAAAAG+GGG - chr2_3:62316837-62316856 Msa0304940:intron 35.0%
!!! TTTTTTGTCCAACGAAAAGG+GGG - chr2_3:62316838-62316857 Msa0304940:intron 35.0%
!!! CTTTGTTTTGTTCAGGTACA+AGG - chr2_3:62317053-62317072 Msa0304940:CDS 35.0%
TTATTAGATGACCAAATCCG+TGG - chr2_3:62317129-62317148 Msa0304940:CDS 35.0%
AGAGAGTAATAGTATTCTCG+TGG - chr2_3:62317690-62317709 Msa0304940:CDS 35.0%
TGCAATGATGAATGCAACTA+AGG - chr2_3:62317713-62317732 Msa0304940:CDS 35.0%
GCAATGATGAATGCAACTAA+GGG - chr2_3:62317714-62317733 Msa0304940:CDS 35.0%
CAATGATGAATGCAACTAAG+GGG - chr2_3:62317715-62317734 Msa0304940:CDS 35.0%
GCCTGAGTAAAGATATTTAG+GGG - chr2_3:62317996-62318015 Msa0304940:CDS 35.0%
CCTGAGTAAAGATATTTAGG+GGG - chr2_3:62317997-62318016 Msa0304940:CDS 35.0%
CTGAGTAAAGATATTTAGGG+GGG - chr2_3:62317998-62318017 Msa0304940:CDS 35.0%
AATCAGTTAAGAGGTTGTCT+TGG - chr2_3:62318033-62318052 Msa0304940:CDS 35.0%
! TCACTGTTTTACGACTTAGA+GGG - chr2_3:62318144-62318163 Msa0304940:five_prime_UTR 35.0%
!!! ACCTTTTTGGCTCGTTTAAT+TGG - chr2_3:62318203-62318222 Msa0304940:five_prime_UTR 35.0%
!!! CCTTTTTGGCTCGTTTAATT+GGG - chr2_3:62318204-62318223 Msa0304940:five_prime_UTR 35.0%
!!! CTTTTTGGCTCGTTTAATTG+GGG - chr2_3:62318205-62318224 Msa0304940:five_prime_UTR 35.0%
! ATGGTTGACTCAGTTCAAAT+TGG - chr2_3:62318269-62318288 Msa0304940:five_prime_UTR 35.0%
GATAAACGCGGTAGATAATG+AGG + chr2_3:62314390-62314409 None:intergenic 40.0%
AATACCTTTGCTTTGACGAG+AGG + chr2_3:62314494-62314513 None:intergenic 40.0%
!! TGAGAAATCGAGTGTCGAAA+TGG - chr2_3:62314558-62314577 Msa0304940:three_prime_UTR 40.0%
GTTAACAATAAAGTCGCGGT+AGG + chr2_3:62314929-62314948 None:intergenic 40.0%
ATTTCGTGCTTGAATCTGTC+GGG + chr2_3:62315088-62315107 None:intergenic 40.0%
TGCACGACATGTTCAATCAA+AGG - chr2_3:62315108-62315127 Msa0304940:intron 40.0%
ATCCACATGCAGAACTGATT+TGG - chr2_3:62315216-62315235 Msa0304940:intron 40.0%
ATCCAAATCAGTTCTGCATG+TGG + chr2_3:62315221-62315240 None:intergenic 40.0%
CACAACAAGACACTGTTATG+CGG + chr2_3:62315252-62315271 None:intergenic 40.0%
AGCCATTCCGGATTTCAAAA+AGG - chr2_3:62315500-62315519 Msa0304940:intron 40.0%
GTTAGGAGAAGAAACTTGGA+AGG + chr2_3:62315528-62315547 None:intergenic 40.0%
! CTAAAGTGGCAGATTTGGTT+AGG + chr2_3:62315545-62315564 None:intergenic 40.0%
!! ATCTGCCACTTTAGCAATAC+AGG - chr2_3:62315551-62315570 Msa0304940:intron 40.0%
TCCATATCGAGATGGTTACT+CGG - chr2_3:62315610-62315629 Msa0304940:intron 40.0%
GTTACTCGGCAACTAGTTAA+TGG - chr2_3:62315624-62315643 Msa0304940:intron 40.0%
TCATGGCTTGAAAGGTGATA+TGG + chr2_3:62315659-62315678 None:intergenic 40.0%
ATGTTCCTTGTCAGCATTCA+TGG + chr2_3:62315676-62315695 None:intergenic 40.0%
TGTAAGCAAATTAGTGGGAG+GGG + chr2_3:62315934-62315953 None:intergenic 40.0%
TGAAGAAAATGTGGGTGGAA+TGG + chr2_3:62316332-62316351 None:intergenic 40.0%
! CAAGTTGAAGAAAATGTGGG+TGG + chr2_3:62316337-62316356 None:intergenic 40.0%
! GCGCAAGTTGAAGAAAATGT+GGG + chr2_3:62316340-62316359 None:intergenic 40.0%
! AGCGCAAGTTGAAGAAAATG+TGG + chr2_3:62316341-62316360 None:intergenic 40.0%
AGTTGAAACGGAGAAAGACA+GGG + chr2_3:62316439-62316458 None:intergenic 40.0%
! GAGGAAGCTATGAGTTGAAA+CGG + chr2_3:62316451-62316470 None:intergenic 40.0%
AGTAGAATTGCCTGTTTGTG+TGG - chr2_3:62316492-62316511 Msa0304940:intron 40.0%
GAGTAACCTTTGTCCAGATA+GGG - chr2_3:62316608-62316627 Msa0304940:intron 40.0%
TCTTCAATGGGAAAACTCCA+TGG + chr2_3:62316692-62316711 None:intergenic 40.0%
! CTGTTCGTCTTGTTCTTCAA+TGG + chr2_3:62316705-62316724 None:intergenic 40.0%
AAGAACAAGACGAACAGTAC+AGG - chr2_3:62316708-62316727 Msa0304940:intron 40.0%
TAAAGTAGCTGAGGGCAAAA+TGG + chr2_3:62316794-62316813 None:intergenic 40.0%
!!! GATTGCGCTTTGTTTTGTTC+AGG - chr2_3:62317046-62317065 Msa0304940:CDS 40.0%
!! TTAGGGGAATTTTTAGAGGG+AGG - chr2_3:62317667-62317686 Msa0304940:intron 40.0%
GGATGATGATCCAGTTATCA+TGG - chr2_3:62317855-62317874 Msa0304940:intron 40.0%
TCTTTCACGTCCATGATAAC+TGG + chr2_3:62317868-62317887 None:intergenic 40.0%
GTTATCATGGACGTGAAAGA+TGG - chr2_3:62317868-62317887 Msa0304940:intron 40.0%
ACAGTTGAGGAAGCCAAATA+TGG - chr2_3:62317924-62317943 Msa0304940:intron 40.0%
CAGTTGAGGAAGCCAAATAT+GGG - chr2_3:62317925-62317944 Msa0304940:intron 40.0%
GTCATGGCAAAACAAGATGA+AGG - chr2_3:62317952-62317971 Msa0304940:intron 40.0%
CCCCCTAAATATCTTTACTC+AGG + chr2_3:62318000-62318019 None:intergenic 40.0%
AAAGATATTTAGGGGGGAAG+TGG - chr2_3:62318004-62318023 Msa0304940:CDS 40.0%
! TGGTGGTGAAATCAGTTAAG+AGG - chr2_3:62318024-62318043 Msa0304940:CDS 40.0%
! GTCACTGTTTTACGACTTAG+AGG - chr2_3:62318143-62318162 Msa0304940:five_prime_UTR 40.0%
! TCGGGCTTAATCAACCTTTT+TGG - chr2_3:62318190-62318209 Msa0304940:five_prime_UTR 40.0%
CCCAATTAAACGAGCCAAAA+AGG + chr2_3:62318207-62318226 None:intergenic 40.0%
GGAGCGATAGAAAACGAACA+AGG + chr2_3:62314433-62314452 None:intergenic 45.0%
! TTTTCTGCGAATTGGGGCTT+AGG - chr2_3:62314530-62314549 Msa0304940:three_prime_UTR 45.0%
TTTCTGCGAATTGGGGCTTA+GGG - chr2_3:62314531-62314550 Msa0304940:three_prime_UTR 45.0%
GTAGCAGAAGACGAAGAAGA+AGG + chr2_3:62314628-62314647 None:intergenic 45.0%
AGCATATGGACGATATCTGC+TGG + chr2_3:62314674-62314693 None:intergenic 45.0%
GATATCGTCCATATGCTCGA+AGG - chr2_3:62314677-62314696 Msa0304940:CDS 45.0%
AACAAAACTCACTCACCAGG+TGG + chr2_3:62314706-62314725 None:intergenic 45.0%
TTGACTAATCTAGCTGCAGG+AGG + chr2_3:62314982-62315001 None:intergenic 45.0%
AGCTTGACTAATCTAGCTGC+AGG + chr2_3:62314985-62315004 None:intergenic 45.0%
! TGCAGCTAGATTAGTCAAGC+TGG - chr2_3:62314984-62315003 Msa0304940:intron 45.0%
! GCAGCTAGATTAGTCAAGCT+GGG - chr2_3:62314985-62315004 Msa0304940:intron 45.0%
AACAAGACACTGTTATGCGG+TGG + chr2_3:62315249-62315268 None:intergenic 45.0%
TTGATGCCAAACATTTGCCC+AGG - chr2_3:62315454-62315473 Msa0304940:intron 45.0%
ATAGCTTCTCCGCCAAATCT+AGG + chr2_3:62315480-62315499 None:intergenic 45.0%
CCATCTCGATATGGACGAAT+TGG + chr2_3:62315605-62315624 None:intergenic 45.0%
CCAATTCGTCCATATCGAGA+TGG - chr2_3:62315602-62315621 Msa0304940:intron 45.0%
GTCAGCATTCATGGCTTGAA+AGG + chr2_3:62315667-62315686 None:intergenic 45.0%
TCAAGCCATGAATGCTGACA+AGG - chr2_3:62315668-62315687 Msa0304940:intron 45.0%
GGATGAAAGAGAGCAGTACT+TGG - chr2_3:62315757-62315776 Msa0304940:intron 45.0%
CTAAAAGCCCCGTGATAAAG+GGG + chr2_3:62316182-62316201 None:intergenic 45.0%
GCTAAAAGCCCCGTGATAAA+GGG + chr2_3:62316183-62316202 None:intergenic 45.0%
GAGTTGAAACGGAGAAAGAC+AGG + chr2_3:62316440-62316459 None:intergenic 45.0%
ATTCTACTACCACCAACGAG+TGG + chr2_3:62316480-62316499 None:intergenic 45.0%
ACGGTCCATTGTTTGCAGAT+GGG + chr2_3:62316525-62316544 None:intergenic 45.0%
AACGGTCCATTGTTTGCAGA+TGG + chr2_3:62316526-62316545 None:intergenic 45.0%
GGAGTAACCTTTGTCCAGAT+AGG - chr2_3:62316607-62316626 Msa0304940:intron 45.0%
AAGTCACCCTATCTGGACAA+AGG + chr2_3:62316617-62316636 None:intergenic 45.0%
GAGACAAAAGTCACCCTATC+TGG + chr2_3:62316624-62316643 None:intergenic 45.0%
AACAGTACAGGCTTGATGCT+TGG - chr2_3:62316720-62316739 Msa0304940:intron 45.0%
ACAGTACAGGCTTGATGCTT+GGG - chr2_3:62316721-62316740 Msa0304940:intron 45.0%
CTGTCGAGCGGTATTCATAA+GGG + chr2_3:62317908-62317927 None:intergenic 45.0%
ACTGTCGAGCGGTATTCATA+AGG + chr2_3:62317909-62317928 None:intergenic 45.0%
GCCAAATATGGGATCTGTCA+TGG - chr2_3:62317936-62317955 Msa0304940:intron 45.0%
GCCATGACAGATCCCATATT+TGG + chr2_3:62317940-62317959 None:intergenic 45.0%
GGTTCCTCTCGTCAAAGCAA+AGG - chr2_3:62314487-62314506 Msa0304940:three_prime_UTR 50.0%
TTCTGCTACTCCTACCTGCT+CGG - chr2_3:62314639-62314658 Msa0304940:three_prime_UTR 50.0%
CAAGCTGGGTTTCGATGATC+AGG - chr2_3:62314999-62315018 Msa0304940:intron 50.0%
TCTAGGCCTGGGCAAATGTT+TGG + chr2_3:62315463-62315482 None:intergenic 50.0%
CATTTGCCCAGGCCTAGATT+TGG - chr2_3:62315465-62315484 Msa0304940:intron 50.0%
CGGAGAAGCTATAGCCATTC+CGG - chr2_3:62315488-62315507 Msa0304940:intron 50.0%
GCCGAGTAACCATCTCGATA+TGG + chr2_3:62315614-62315633 None:intergenic 50.0%
GGCTAAAAGCCCCGTGATAA+AGG + chr2_3:62316184-62316203 None:intergenic 50.0%
GGCGGCAATATTAGAGTTGC+TGG - chr2_3:62316391-62316410 Msa0304940:intron 50.0%
TTCCTCTCCATTCCACTCGT+TGG - chr2_3:62316465-62316484 Msa0304940:intron 50.0%
ACTACCACCAACGAGTGGAA+TGG + chr2_3:62316475-62316494 None:intergenic 50.0%
ATTGTTTGCAGATGGGGGGA+AGG + chr2_3:62316518-62316537 None:intergenic 50.0%
TTCCCCCCATCTGCAAACAA+TGG - chr2_3:62316517-62316536 Msa0304940:intron 50.0%
GTCCATTGTTTGCAGATGGG+GGG + chr2_3:62316522-62316541 None:intergenic 50.0%
GGTCCATTGTTTGCAGATGG+GGG + chr2_3:62316523-62316542 None:intergenic 50.0%
CGGTCCATTGTTTGCAGATG+GGG + chr2_3:62316524-62316543 None:intergenic 50.0%
! TGGGAAATGAATGATGGGGC+AGG - chr2_3:62316567-62316586 Msa0304940:intron 50.0%
AAACTCCATGGCTGAGTAGG+AGG + chr2_3:62316680-62316699 None:intergenic 50.0%
GGAAAACTCCATGGCTGAGT+AGG + chr2_3:62316683-62316702 None:intergenic 50.0%
AAAAGGGGGACAAGGCTAGT+GGG - chr2_3:62316852-62316871 Msa0304940:intron 50.0%
TCTCATGCAGCTGGTAAGTG+AGG - chr2_3:62317080-62317099 Msa0304940:CDS 50.0%
GTGTGTGTGAACCACGGATT+TGG + chr2_3:62317143-62317162 None:intergenic 50.0%
GTGTGTGTGTGTGTGAACCA+CGG + chr2_3:62317149-62317168 None:intergenic 50.0%
TGAATACCGCTCGACAGTTG+AGG - chr2_3:62317911-62317930 Msa0304940:intron 50.0%
GATATTTAGGGGGGAAGTGG+TGG - chr2_3:62318007-62318026 Msa0304940:CDS 50.0%
GGACGGTGAAGAACGAACGA+GGG + chr2_3:62314454-62314473 None:intergenic 55.0%
AGGACGGTGAAGAACGAACG+AGG + chr2_3:62314455-62314474 None:intergenic 55.0%
TTCTTCACCGTCCTGTCCTG+CGG - chr2_3:62314461-62314480 Msa0304940:three_prime_UTR 55.0%
AACCAAACCGCAGGACAGGA+CGG + chr2_3:62314471-62314490 None:intergenic 55.0%
GGTGGTGACCTTCGAGCATA+TGG + chr2_3:62314688-62314707 None:intergenic 55.0%
TATGCTCGAAGGTCACCACC+TGG - chr2_3:62314688-62314707 Msa0304940:CDS 55.0%
AATCTAGCTGCAGGAGGCGA+TGG + chr2_3:62314976-62314995 None:intergenic 55.0%
TCGTGCTTGAATCTGTCGGG+CGG + chr2_3:62315085-62315104 None:intergenic 55.0%
TTGCCCAGGCCTAGATTTGG+CGG - chr2_3:62315468-62315487 Msa0304940:intron 55.0%
TCTCCGCCAAATCTAGGCCT+GGG + chr2_3:62315474-62315493 None:intergenic 55.0%
TTCTCCGCCAAATCTAGGCC+TGG + chr2_3:62315475-62315494 None:intergenic 55.0%
ATTGCCGCCAAACTGCTCAG+TGG + chr2_3:62316380-62316399 None:intergenic 55.0%
CACCAACGAGTGGAATGGAG+AGG + chr2_3:62316470-62316489 None:intergenic 55.0%
CTCTCCATTCCACTCGTTGG+TGG - chr2_3:62316468-62316487 Msa0304940:intron 55.0%
GTCCAACGAAAAGGGGGACA+AGG - chr2_3:62316844-62316863 Msa0304940:intron 55.0%
!! AGCCTTGTCCCCCTTTTCGT+TGG + chr2_3:62316849-62316868 None:intergenic 55.0%
GAAAAGGGGGACAAGGCTAG+TGG - chr2_3:62316851-62316870 Msa0304940:intron 55.0%
AAAGGGGGACAAGGCTAGTG+GGG - chr2_3:62316853-62316872 Msa0304940:intron 55.0%
TGGCTTCCTCAACTGTCGAG+CGG + chr2_3:62317920-62317939 None:intergenic 55.0%
GAGGAACCAAACCGCAGGAC+AGG + chr2_3:62314475-62314494 None:intergenic 60.0%
GACGAGAGGAACCAAACCGC+AGG + chr2_3:62314480-62314499 None:intergenic 60.0%
TGCTACTCCTACCTGCTCGG+AGG - chr2_3:62314642-62314661 Msa0304940:three_prime_UTR 60.0%
GCTTTCACCTCCGAGCAGGT+AGG + chr2_3:62314652-62314671 None:intergenic 60.0%
GCTGCAGGAGGCGATGGATA+TGG + chr2_3:62314970-62314989 None:intergenic 60.0%
CGTGCTTGAATCTGTCGGGC+GGG + chr2_3:62315084-62315103 None:intergenic 60.0%
AAACTGCTCAGTGGCCGGTG+GGG + chr2_3:62316371-62316390 None:intergenic 60.0%
CAAACTGCTCAGTGGCCGGT+GGG + chr2_3:62316372-62316391 None:intergenic 60.0%
!! CAAGGGCACTCTCATGCAGC+TGG - chr2_3:62317071-62317090 Msa0304940:CDS 60.0%
!! GATGAAGGCGCTGAAGCTGC+TGG - chr2_3:62317967-62317986 Msa0304940:intron 60.0%
CACCGTCCTGTCCTGCGGTT+TGG - chr2_3:62314466-62314485 Msa0304940:three_prime_UTR 65.0%
!! GCTGGCTTTCACCTCCGAGC+AGG + chr2_3:62314656-62314675 None:intergenic 65.0%
TTGCGCTCCACCTTCCCCAC+CGG - chr2_3:62316354-62316373 Msa0304940:intron 65.0%
CCAAACTGCTCAGTGGCCGG+TGG + chr2_3:62316373-62316392 None:intergenic 65.0%
CCACCGGCCACTGAGCAGTT+TGG - chr2_3:62316370-62316389 Msa0304940:intron 65.0%
CCGCCAAACTGCTCAGTGGC+CGG + chr2_3:62316376-62316395 None:intergenic 65.0%
CCGGCCACTGAGCAGTTTGG+CGG - chr2_3:62316373-62316392 Msa0304940:intron 65.0%
GGGGGGAAGGCCACACAAAC+AGG + chr2_3:62316505-62316524 None:intergenic 65.0%
TGGGGCAGGTCTGCGTGAAC+AGG - chr2_3:62316581-62316600 Msa0304940:intron 65.0%
GGGGCAGGTCTGCGTGAACA+GGG - chr2_3:62316582-62316601 Msa0304940:intron 65.0%
CAGGTCTGCGTGAACAGGGC+TGG - chr2_3:62316586-62316605 Msa0304940:intron 65.0%
GGCTGCCTCCTACTCAGCCA+TGG - chr2_3:62316672-62316691 Msa0304940:intron 65.0%
TCAGTGGCCGGTGGGGAAGG+TGG + chr2_3:62316364-62316383 None:intergenic 70.0%
TGCTCAGTGGCCGGTGGGGA+AGG + chr2_3:62316367-62316386 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 62314371 62318309 62314371 ID=Msa0304940;Name=Msa0304940
chr2_3 mRNA 62314371 62318309 62314371 ID=Msa0304940-mRNA-1;Parent=Msa0304940;Name=Msa0304940-mRNA-1;_AED=0.34;_eAED=0.34;_QI=206|0.6|0.5|1|0.6|0.5|6|304|219
chr2_3 exon 62314371 62314827 62314371 ID=Msa0304940-mRNA-1:exon:20800;Parent=Msa0304940-mRNA-1
chr2_3 exon 62315588 62315612 62315588 ID=Msa0304940-mRNA-1:exon:20799;Parent=Msa0304940-mRNA-1
chr2_3 exon 62316902 62316936 62316902 ID=Msa0304940-mRNA-1:exon:20798;Parent=Msa0304940-mRNA-1
chr2_3 exon 62316967 62317178 62316967 ID=Msa0304940-mRNA-1:exon:20797;Parent=Msa0304940-mRNA-1
chr2_3 exon 62317674 62317775 62317674 ID=Msa0304940-mRNA-1:exon:20796;Parent=Msa0304940-mRNA-1
chr2_3 exon 62317971 62318309 62317971 ID=Msa0304940-mRNA-1:exon:20795;Parent=Msa0304940-mRNA-1
chr2_3 five_prime_UTR 62318104 62318309 62318104 ID=Msa0304940-mRNA-1:five_prime_utr;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62317971 62318103 62317971 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62317674 62317775 62317674 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62316967 62317178 62316967 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62316902 62316936 62316902 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62315588 62315612 62315588 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 CDS 62314675 62314827 62314675 ID=Msa0304940-mRNA-1:cds;Parent=Msa0304940-mRNA-1
chr2_3 three_prime_UTR 62314371 62314674 62314371 ID=Msa0304940-mRNA-1:three_prime_utr;Parent=Msa0304940-mRNA-1
Gene Sequence

>Msa0304940

ATGGAATTGAACAAACCCTCAGACTTACTTCAAAAAATGCAACATCATCCTTCTTCTTCGTCTTCTGCTACTCCTACCTGCTCGGAGGTGAAAGCCAGCAGATATCGTCCATATGCTCGAAGGTCACCACCTGATCCGAATCAAATTAGAAAATCCTACCGCGACTTTATTGTTAACGTTAATCTTGACGATATACCATATCCATCGCCTCCTGCAGCTAGATTAGTCAAGCTGGCCATTCCGGATTTCAAAAAGGATCCTTCCAAGTTTCTTCTCCTAACCAAATCTGCCACTTTAGCAATACAGGATTATAATTCACAAACTCTCACAGACTACCAATTCGTCCATATCGAGATGGTTACTCGGCAACTAGTTAATGGTATCCTTTACCATATCACCTTTCAAGCCATGAATGCTGACAAGGAACATGCAACTTTCGAAGCCACAGTTAAGAGAATCAGGATGAAAGAGAGCAGTACTTGGTACAAGGGCACTCTCATGCAGCTGGAGGATGATGATCCAGTTATCATGGACGTGAAAGATGGTAATGACGTTTATGCCCTTATGAATACCGCTCGACAGTTGAGGAAGCCAAATATGGGATCTGTCATGGCAAAACAAGATGAAGGCGCTGAAGCTGCTGGAGCATTGCCTGAGTAA
Protein sequence

>Msa0304940

MELNKPSDLLQKMQHHPSSSSSATPTCSEVKASRYRPYARRSPPDPNQIRKSYRDFIVNVNLDDIPYPSPPAARLVKLAIPDFKKDPSKFLLLTKSATLAIQDYNSQTLTDYQFVHIEMVTRQLVNGILYHITFQAMNADKEHATFEATVKRIRMKESSTWYKGTLMQLEDDDPVIMDVKDGNDVYALMNTARQLRKPNMGSVMAKQDEGAEAAGALPE*