AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0305070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0305070 MtrunA17_Chr2g0326101 49.388 245 81 8 1 214 232 464 1.51e-55 183
Msa0305070 MtrunA17_Chr2g0326101 46.122 245 78 7 1 208 1 228 1.46e-47 162
Msa0305070 MtrunA17_Chr2g0319901 61.074 149 44 2 85 219 80 228 1.65e-55 176
Msa0305070 MtrunA17_Chr2g0333201 49.798 247 84 8 1 219 3 237 1.93e-55 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCTGAGTAAAGATATTT+AGG 0.147252 2_3:-62494515 Msa0305070:exon
GCCATGACAGATCCCATATT+TGG 0.209504 2_3:+62494572 None:intergenic
ATGTTCCTTGTCAGCATTCA+TGG 0.213484 2_3:+62496847 None:intergenic
TTAAATGAATTCTATTTATT+AGG 0.246763 2_3:+62494275 None:intergenic
CCCAATTAAACGAGCCAAAA+AGG 0.289069 2_3:+62494306 None:intergenic
TTAAGCCCGAGAAATAATTA+AGG 0.291848 2_3:+62494333 None:intergenic
ACAGTTGAGGAAGCCAAATA+TGG 0.300778 2_3:-62494585 Msa0305070:CDS
TGAGTTGTATGTTAACTCTT+AGG 0.314820 2_3:-62494417 Msa0305070:three_prime_UTR
TCAGAAAGGTTAAGAGAATC+AGG 0.328389 2_3:-62496784 Msa0305070:intron
TATTGCTAAAGTGGCAGATT+TGG 0.335525 2_3:+62496973 None:intergenic
AGCCATTCCGGATTTCAAAA+AGG 0.338321 2_3:-62497020 Msa0305070:CDS
CACCGTCCTGTCCTGCGGTT+TGG 0.338544 2_3:-62498054 Msa0305070:five_prime_UTR
GTTACTCGGCAACTAGTTAA+TGG 0.344977 2_3:-62496896 Msa0305070:CDS
CGGAGAAGCTATAGCCATTC+CGG 0.350939 2_3:-62497032 Msa0305070:intron
GCTGCAGGAGGCGATGGATA+TGG 0.362176 2_3:+62497553 None:intergenic
ATCTGCCACTTTAGCAATAC+AGG 0.375351 2_3:-62496969 Msa0305070:CDS
TCATGGCTTGAAAGGTGATA+TGG 0.376146 2_3:+62496864 None:intergenic
TCTTTCACGTCCATGATAAC+TGG 0.380417 2_3:+62494644 None:intergenic
CTAAAGTGGCAGATTTGGTT+AGG 0.389297 2_3:+62496978 None:intergenic
TAGATCCCTTAATTATTTCT+CGG 0.401123 2_3:-62494339 Msa0305070:three_prime_UTR
TGCCTGAGTAAAGATATTTA+GGG 0.406335 2_3:-62494514 Msa0305070:exon
CCATCTCGATATGGACGAAT+TGG 0.412531 2_3:+62496918 None:intergenic
GGTGGTGACCTTCGAGCATA+TGG 0.427486 2_3:+62497835 None:intergenic
ATGGTTGACTCAGTTCAAAT+TGG 0.433101 2_3:-62494241 Msa0305070:three_prime_UTR
ATAATCCTGTATTGCTAAAG+TGG 0.435729 2_3:+62496964 None:intergenic
TGCAGCTAGATTAGTCAAGC+TGG 0.441470 2_3:-62497536 Msa0305070:CDS
TTTCTGCGAATTGGGGCTTA+GGG 0.442584 2_3:-62497989 Msa0305070:five_prime_UTR
GCCGAGTAACCATCTCGATA+TGG 0.446899 2_3:+62496909 None:intergenic
ACTGTCGAGCGGTATTCATA+AGG 0.452038 2_3:+62494603 None:intergenic
AGATCCCTTAATTATTTCTC+GGG 0.456806 2_3:-62494338 Msa0305070:three_prime_UTR
GATGAAGGCGCTGAAGCTGC+TGG 0.460674 2_3:-62494542 Msa0305070:CDS
TGAGAAATCGAGTGTCGAAA+TGG 0.464953 2_3:-62497962 Msa0305070:exon
GAGGAACCAAACCGCAGGAC+AGG 0.465131 2_3:+62498048 None:intergenic
TTTGGTTAGGAGAAGAAACT+TGG 0.474276 2_3:+62496991 None:intergenic
TTCTGCTACTCCTACCTGCT+CGG 0.483294 2_3:-62497881 Msa0305070:CDS
TGGTGGTGAAATCAGTTAAG+AGG 0.489867 2_3:-62494486 Msa0305070:three_prime_UTR
CTGTCGAGCGGTATTCATAA+GGG 0.496417 2_3:+62494604 None:intergenic
GGATGATGATCCAGTTATCA+TGG 0.510725 2_3:-62494654 Msa0305070:CDS
TAAGCCCGAGAAATAATTAA+GGG 0.512273 2_3:+62494334 None:intergenic
CTTGAAAGGTGATATGGTAA+AGG 0.513491 2_3:+62496870 None:intergenic
AATCAGTTAAGAGGTTGTCT+TGG 0.524040 2_3:-62494477 Msa0305070:three_prime_UTR
AATCTAGCTGCAGGAGGCGA+TGG 0.532648 2_3:+62497547 None:intergenic
GCTGGCTTTCACCTCCGAGC+AGG 0.533675 2_3:+62497867 None:intergenic
CAGTTGAGGAAGCCAAATAT+GGG 0.536691 2_3:-62494584 Msa0305070:CDS
AGCATATGGACGATATCTGC+TGG 0.543046 2_3:+62497849 None:intergenic
GCAGCTAGATTAGTCAAGCT+GGG 0.544326 2_3:-62497535 Msa0305070:intron
GTCAGCATTCATGGCTTGAA+AGG 0.544835 2_3:+62496856 None:intergenic
AGCTTGACTAATCTAGCTGC+AGG 0.550250 2_3:+62497538 None:intergenic
CAAGGGCACTCTCATGCAGC+TGG 0.551659 2_3:-62495448 Msa0305070:intron
GTTATCATGGACGTGAAAGA+TGG 0.553405 2_3:-62494641 Msa0305070:CDS
AATACCTTTGCTTTGACGAG+AGG 0.555040 2_3:+62498029 None:intergenic
GGATGAAAGAGAGCAGTACT+TGG 0.557944 2_3:-62496763 Msa0305070:intron
TGAATACCGCTCGACAGTTG+AGG 0.564621 2_3:-62494598 Msa0305070:CDS
GTAGCAGAAGACGAAGAAGA+AGG 0.565840 2_3:+62497895 None:intergenic
GCCAAATATGGGATCTGTCA+TGG 0.570040 2_3:-62494573 Msa0305070:CDS
TTCTTCACCGTCCTGTCCTG+CGG 0.571363 2_3:-62498059 Msa0305070:five_prime_UTR
GTCATGGCAAAACAAGATGA+AGG 0.571769 2_3:-62494557 Msa0305070:CDS
GTTAGGAGAAGAAACTTGGA+AGG 0.575655 2_3:+62496995 None:intergenic
GATAAACGCGGTAGATAATG+AGG 0.581551 2_3:+62498133 None:intergenic
GGTTCCTCTCGTCAAAGCAA+AGG 0.590634 2_3:-62498033 Msa0305070:five_prime_UTR
TCAAGCCATGAATGCTGACA+AGG 0.596210 2_3:-62496852 Msa0305070:CDS
TCCATATCGAGATGGTTACT+CGG 0.600828 2_3:-62496910 Msa0305070:CDS
CCAATTCGTCCATATCGAGA+TGG 0.612639 2_3:-62496918 Msa0305070:CDS
GCCTGAGTAAAGATATTTAG+GGG 0.617144 2_3:-62494513 Msa0305070:exon
AGGACGGTGAAGAACGAACG+AGG 0.618237 2_3:+62498068 None:intergenic
GACGAGAGGAACCAAACCGC+AGG 0.619819 2_3:+62498043 None:intergenic
GTTAACAATAAAGTCGCGGT+AGG 0.622455 2_3:+62497594 None:intergenic
TATGCTCGAAGGTCACCACC+TGG 0.625018 2_3:-62497832 Msa0305070:intron
AACCAAACCGCAGGACAGGA+CGG 0.629740 2_3:+62498052 None:intergenic
TGGCTTCCTCAACTGTCGAG+CGG 0.636593 2_3:+62494592 None:intergenic
GCTTTCACCTCCGAGCAGGT+AGG 0.649215 2_3:+62497871 None:intergenic
GATATCGTCCATATGCTCGA+AGG 0.658364 2_3:-62497843 Msa0305070:CDS
GGAGCGATAGAAAACGAACA+AGG 0.668006 2_3:+62498090 None:intergenic
CCTGAGTAAAGATATTTAGG+GGG 0.669853 2_3:-62494512 Msa0305070:exon
TGCTACTCCTACCTGCTCGG+AGG 0.671023 2_3:-62497878 Msa0305070:CDS
TTGACTAATCTAGCTGCAGG+AGG 0.674170 2_3:+62497541 None:intergenic
TAACGTTAACAATAAAGTCG+CGG 0.689934 2_3:+62497590 None:intergenic
GGACGGTGAAGAACGAACGA+GGG 0.707025 2_3:+62498069 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATGAATTCTATTTATT+AGG + chr2_3:62498100-62498119 None:intergenic 10.0%
!!! ATATTGTTTGTTATTGTTAA+TGG - chr2_3:62494612-62494631 Msa0305070:CDS 15.0%
!! TGAAAAGATCATAAAATAAT+GGG + chr2_3:62495856-62495875 None:intergenic 15.0%
!! ATGAAAAGATCATAAAATAA+TGG + chr2_3:62495857-62495876 None:intergenic 15.0%
!!! TGTTGATAATGTTTTTAAAT+AGG + chr2_3:62495901-62495920 None:intergenic 15.0%
!! AAAAAAAAAAACACATTACA+GGG + chr2_3:62495964-62495983 None:intergenic 15.0%
!! TAAAAAAAAAAACACATTAC+AGG + chr2_3:62495965-62495984 None:intergenic 15.0%
!! GTGTTTGTATATATTAAAAA+CGG - chr2_3:62495998-62496017 Msa0305070:intron 15.0%
!! TATTTCTTTATATATAGACA+AGG + chr2_3:62496092-62496111 None:intergenic 15.0%
!!! TTATTAATCTTTTCAACAAT+GGG - chr2_3:62496497-62496516 Msa0305070:intron 15.0%
!!! ATATAATTCTTTGTTTTAGA+AGG - chr2_3:62496605-62496624 Msa0305070:intron 15.0%
!!! AAGCAAATAATATTTTTGTT+AGG + chr2_3:62496745-62496764 None:intergenic 15.0%
!! ATCTAATAATTGTAAGAAAA+AGG + chr2_3:62496971-62496990 None:intergenic 15.0%
!! ATTAAAGTAATGATATTGTT+AGG - chr2_3:62497512-62497531 Msa0305070:intron 15.0%
!! TTAAAGTAATGATATTGTTA+GGG - chr2_3:62497513-62497532 Msa0305070:intron 15.0%
!!! GTTGATTTTTTTTTTAATTC+AGG - chr2_3:62497694-62497713 Msa0305070:intron 15.0%
!! TCATTTAATGTTAAAAAGAA+TGG - chr2_3:62498112-62498131 Msa0305070:five_prime_UTR 15.0%
!!! TAGGATTTTCTAATTTGATT+CGG + chr2_3:62494762-62494781 None:intergenic 20.0%
!! AACATGATAATAATTGCAAT+AGG + chr2_3:62494892-62494911 None:intergenic 20.0%
!! GGATTATCTATGTATTTATT+TGG + chr2_3:62495052-62495071 None:intergenic 20.0%
!! ATTTCTCATAAGAAAAATCT+AGG - chr2_3:62495124-62495143 Msa0305070:intron 20.0%
!! TGTATGCAAAGAAAAATATT+GGG + chr2_3:62495702-62495721 None:intergenic 20.0%
!! ATGTATGCAAAGAAAAATAT+TGG + chr2_3:62495703-62495722 None:intergenic 20.0%
!! AAAAAAAAAACACATTACAG+GGG + chr2_3:62495963-62495982 None:intergenic 20.0%
!! AATATATACAAACACATTAC+AGG + chr2_3:62495993-62496012 None:intergenic 20.0%
!!! TAGTTTTTCAAGAGAAAAAA+GGG + chr2_3:62496066-62496085 None:intergenic 20.0%
!!! CTTATTAATCTTTTCAACAA+TGG - chr2_3:62496496-62496515 Msa0305070:intron 20.0%
!! ATGTATCTAGTCTATAATAT+TGG + chr2_3:62497135-62497154 None:intergenic 20.0%
!!! ATCAACTTTTTCATTTTTTG+AGG - chr2_3:62497259-62497278 Msa0305070:intron 20.0%
!!! TCAACTTTTTCATTTTTTGA+GGG - chr2_3:62497260-62497279 Msa0305070:intron 20.0%
!! TGAAAAAGAAACACTAAAAT+TGG + chr2_3:62497354-62497373 None:intergenic 20.0%
!! TAAAGTAATGATATTGTTAG+GGG - chr2_3:62497514-62497533 Msa0305070:intron 20.0%
!! ATAAAATGAGCATCAATATT+AGG - chr2_3:62497608-62497627 Msa0305070:CDS 20.0%
!!! GATTTTTTTTTTAATTCAGG+AGG - chr2_3:62497697-62497716 Msa0305070:intron 20.0%
!!! ATTTTTTGAAGTAAGTCTGA+GGG + chr2_3:62494447-62494466 None:intergenic 25.0%
!!! TGTTTGTTATTGTTAATGGA+TGG - chr2_3:62494616-62494635 Msa0305070:CDS 25.0%
! AAAAGTAGAGAAACAAACAA+TGG + chr2_3:62494730-62494749 None:intergenic 25.0%
! TATGCATAACATAAGCTATA+AGG + chr2_3:62495008-62495027 None:intergenic 25.0%
!! TAAGAGCAAAATTTTCAGAA+AGG - chr2_3:62495574-62495593 Msa0305070:intron 25.0%
!! TTTTCTTTGCATGACATTTT+TGG - chr2_3:62495730-62495749 Msa0305070:intron 25.0%
! TACAATGTAAGCAAATTAGT+GGG + chr2_3:62495792-62495811 None:intergenic 25.0%
! TTACAATGTAAGCAAATTAG+TGG + chr2_3:62495793-62495812 None:intergenic 25.0%
!!! TTTAGCTTTTGCTTTTTTCT+TGG - chr2_3:62495814-62495833 Msa0305070:intron 25.0%
!! ATAAAATAATGGGAACGAAA+GGG + chr2_3:62495846-62495865 None:intergenic 25.0%
!!! CTAGTTTTTCAAGAGAAAAA+AGG + chr2_3:62496067-62496086 None:intergenic 25.0%
! ACTTACCTACAACAATTAAA+CGG + chr2_3:62496397-62496416 None:intergenic 25.0%
!! TAAGCAAAAAACAACGTTTT+AGG - chr2_3:62497084-62497103 Msa0305070:intron 25.0%
! ATCTAGTCTATAATATTGGT+TGG + chr2_3:62497131-62497150 None:intergenic 25.0%
!!! GTTGCTAGAATTCATTTTTT+AGG + chr2_3:62497173-62497192 None:intergenic 25.0%
!! ATTGGTAACAGAAAATTTAG+AGG + chr2_3:62497336-62497355 None:intergenic 25.0%
!! TGTGTTTTATGTTAAAAACG+TGG - chr2_3:62497921-62497940 Msa0305070:CDS 25.0%
!! GTGTTTTATGTTAAAAACGT+GGG - chr2_3:62497922-62497941 Msa0305070:CDS 25.0%
!! TAGATCCCTTAATTATTTCT+CGG - chr2_3:62498033-62498052 Msa0305070:five_prime_UTR 25.0%
AAAAATCAACTCGATAAACG+CGG + chr2_3:62494254-62494273 None:intergenic 30.0%
!! TACACTATTTTTCTGCGAAT+TGG - chr2_3:62494374-62494393 Msa0305070:three_prime_UTR 30.0%
!! ACACTATTTTTCTGCGAATT+GGG - chr2_3:62494375-62494394 Msa0305070:three_prime_UTR 30.0%
!!! CATTTTTTGAAGTAAGTCTG+AGG + chr2_3:62494448-62494467 None:intergenic 30.0%
GGATGGTTGAATGAATTTAA+TGG - chr2_3:62494633-62494652 Msa0305070:CDS 30.0%
AGCTAGCTAATTCTCTATAA+TGG + chr2_3:62494675-62494694 None:intergenic 30.0%
TAACGTTAACAATAAAGTCG+CGG + chr2_3:62494785-62494804 None:intergenic 30.0%
AAAGGTTTATGATTGTGAAC+AGG + chr2_3:62495268-62495287 None:intergenic 30.0%
!!! AAACCTTTTTTCTTCTTCAC+AGG - chr2_3:62495280-62495299 Msa0305070:intron 30.0%
AAACCTGTGAAGAAGAAAAA+AGG + chr2_3:62495286-62495305 None:intergenic 30.0%
ATAATCCTGTATTGCTAAAG+TGG + chr2_3:62495411-62495430 None:intergenic 30.0%
! CATAAAATAATGGGAACGAA+AGG + chr2_3:62495847-62495866 None:intergenic 30.0%
AGTTAATATCCCCTTTATCA+CGG - chr2_3:62496024-62496043 Msa0305070:intron 30.0%
TCAAGAGAAAAAAGGGAATA+GGG + chr2_3:62496059-62496078 None:intergenic 30.0%
TTCAAGAGAAAAAAGGGAAT+AGG + chr2_3:62496060-62496079 None:intergenic 30.0%
CTTTGATCAAAACGACTAAT+AGG + chr2_3:62496128-62496147 None:intergenic 30.0%
AATTGTTGTAGGTAAGTTGT+TGG - chr2_3:62496400-62496419 Msa0305070:intron 30.0%
ATTGTTGTAGGTAAGTTGTT+GGG - chr2_3:62496401-62496420 Msa0305070:intron 30.0%
ATGAAATGTAAAGTAGCTGA+GGG + chr2_3:62496655-62496674 None:intergenic 30.0%
!!! AGTTTTTTGTCCAACGAAAA+GGG - chr2_3:62496689-62496708 Msa0305070:intron 30.0%
CTCTTGAAGAATAAAGGTAA+AGG + chr2_3:62496808-62496827 None:intergenic 30.0%
AGAAGACTCTTGAAGAATAA+AGG + chr2_3:62496814-62496833 None:intergenic 30.0%
ATTTAGCGTATCTACTCATT+TGG - chr2_3:62496848-62496867 Msa0305070:CDS 30.0%
GGTTCTTGTTCACATAATTA+CGG - chr2_3:62496869-62496888 Msa0305070:CDS 30.0%
GTTCTTGTTCACATAATTAC+GGG - chr2_3:62496870-62496889 Msa0305070:CDS 30.0%
!!! TTTGTTTTGTTCAGGTACAA+GGG - chr2_3:62496907-62496926 Msa0305070:CDS 30.0%
TGCTTATAATAGTGACTGAA+GGG + chr2_3:62497070-62497089 None:intergenic 30.0%
TTGCTTATAATAGTGACTGA+AGG + chr2_3:62497071-62497090 None:intergenic 30.0%
TGGTTGGATACATCATTTAT+TGG + chr2_3:62497115-62497134 None:intergenic 30.0%
!! ATTGTTAGGGGAATTTTTAG+AGG - chr2_3:62497526-62497545 Msa0305070:intron 30.0%
!! TTGTTAGGGGAATTTTTAGA+GGG - chr2_3:62497527-62497546 Msa0305070:intron 30.0%
TTGCCTGAGTAAAGATATTT+AGG - chr2_3:62497857-62497876 Msa0305070:CDS 30.0%
TGCCTGAGTAAAGATATTTA+GGG - chr2_3:62497858-62497877 Msa0305070:CDS 30.0%
TGAGTTGTATGTTAACTCTT+AGG - chr2_3:62497955-62497974 Msa0305070:exon 30.0%
! AGATCCCTTAATTATTTCTC+GGG - chr2_3:62498034-62498053 Msa0305070:five_prime_UTR 30.0%
TAAGCCCGAGAAATAATTAA+GGG + chr2_3:62498041-62498060 None:intergenic 30.0%
TTAAGCCCGAGAAATAATTA+AGG + chr2_3:62498042-62498061 None:intergenic 30.0%
!! CACTATTTTTCTGCGAATTG+GGG - chr2_3:62494376-62494395 Msa0305070:three_prime_UTR 35.0%
TTAAACAAAACTCACTCACC+AGG + chr2_3:62494561-62494580 None:intergenic 35.0%
AATTTCGTGCTTGAATCTGT+CGG + chr2_3:62494941-62494960 None:intergenic 35.0%
TGTCATCGAATATGCACAAA+TGG + chr2_3:62495219-62495238 None:intergenic 35.0%
!!! ATCCTTTTTGAAATCCGGAA+TGG + chr2_3:62495357-62495376 None:intergenic 35.0%
!!! GAAGGATCCTTTTTGAAATC+CGG + chr2_3:62495362-62495381 None:intergenic 35.0%
TTTGGTTAGGAGAAGAAACT+TGG + chr2_3:62495384-62495403 None:intergenic 35.0%
TATTGCTAAAGTGGCAGATT+TGG + chr2_3:62495402-62495421 None:intergenic 35.0%
CTTGAAAGGTGATATGGTAA+AGG + chr2_3:62495505-62495524 None:intergenic 35.0%
! TTTGCTCTTATTAAGCACTG+TGG + chr2_3:62495564-62495583 None:intergenic 35.0%
TCAGAAAGGTTAAGAGAATC+AGG - chr2_3:62495588-62495607 Msa0305070:intron 35.0%
ATGTAAGCAAATTAGTGGGA+GGG + chr2_3:62495788-62495807 None:intergenic 35.0%
AATGTAAGCAAATTAGTGGG+AGG + chr2_3:62495789-62495808 None:intergenic 35.0%
TCATCTGTTCGTATGCAATT+TGG - chr2_3:62495873-62495892 Msa0305070:intron 35.0%
CATCTGTTCGTATGCAATTT+GGG - chr2_3:62495874-62495893 Msa0305070:intron 35.0%
GTTAATATCCCCTTTATCAC+GGG - chr2_3:62496025-62496044 Msa0305070:intron 35.0%
TTAATATCCCCTTTATCACG+GGG - chr2_3:62496026-62496045 Msa0305070:intron 35.0%
GGTTAAATGCATAAATTGCG+TGG + chr2_3:62496165-62496184 None:intergenic 35.0%
ATGGACCGTTTAATTGTTGT+AGG - chr2_3:62496389-62496408 Msa0305070:intron 35.0%
AGTTGTTGGGAAATGAATGA+TGG - chr2_3:62496414-62496433 Msa0305070:intron 35.0%
GTTGTTGGGAAATGAATGAT+GGG - chr2_3:62496415-62496434 Msa0305070:intron 35.0%
TTGTTGGGAAATGAATGATG+GGG - chr2_3:62496416-62496435 Msa0305070:intron 35.0%
! ATCTTTTCAACAATGGGTGA+TGG - chr2_3:62496503-62496522 Msa0305070:intron 35.0%
! TCTTTTCAACAATGGGTGAT+GGG - chr2_3:62496504-62496523 Msa0305070:intron 35.0%
! TGTTCGTCTTGTTCTTCAAT+GGG + chr2_3:62496557-62496576 None:intergenic 35.0%
CATGAAATGTAAAGTAGCTG+AGG + chr2_3:62496656-62496675 None:intergenic 35.0%
!!! GAGTTTTTTGTCCAACGAAA+AGG - chr2_3:62496688-62496707 Msa0305070:intron 35.0%
!!! GTTTTTTGTCCAACGAAAAG+GGG - chr2_3:62496690-62496709 Msa0305070:intron 35.0%
!!! TTTTTTGTCCAACGAAAAGG+GGG - chr2_3:62496691-62496710 Msa0305070:intron 35.0%
!!! CTTTGTTTTGTTCAGGTACA+AGG - chr2_3:62496906-62496925 Msa0305070:CDS 35.0%
TTATTAGATGACCAAATCCG+TGG - chr2_3:62496982-62497001 Msa0305070:CDS 35.0%
AGAGAGTAATAGTATTCTCG+TGG - chr2_3:62497553-62497572 Msa0305070:CDS 35.0%
TGCAATGATGAATGCAACTA+AGG - chr2_3:62497576-62497595 Msa0305070:CDS 35.0%
GCAATGATGAATGCAACTAA+GGG - chr2_3:62497577-62497596 Msa0305070:CDS 35.0%
CAATGATGAATGCAACTAAG+GGG - chr2_3:62497578-62497597 Msa0305070:CDS 35.0%
GCCTGAGTAAAGATATTTAG+GGG - chr2_3:62497859-62497878 Msa0305070:CDS 35.0%
CCTGAGTAAAGATATTTAGG+GGG - chr2_3:62497860-62497879 Msa0305070:CDS 35.0%
TAAAGATATTTAGGGGGAAG+TGG - chr2_3:62497866-62497885 Msa0305070:CDS 35.0%
AATCAGTTAAGAGGTTGTCT+TGG - chr2_3:62497895-62497914 Msa0305070:CDS 35.0%
! TCACTGTTTTACGACTTAGA+GGG - chr2_3:62498006-62498025 Msa0305070:five_prime_UTR 35.0%
!!! ACCTTTTTGGCTCGTTTAAT+TGG - chr2_3:62498065-62498084 Msa0305070:five_prime_UTR 35.0%
!!! CCTTTTTGGCTCGTTTAATT+GGG - chr2_3:62498066-62498085 Msa0305070:five_prime_UTR 35.0%
!!! CTTTTTGGCTCGTTTAATTG+GGG - chr2_3:62498067-62498086 Msa0305070:five_prime_UTR 35.0%
! ATGGTTGACTCAGTTCAAAT+TGG - chr2_3:62498131-62498150 Msa0305070:five_prime_UTR 35.0%
GATAAACGCGGTAGATAATG+AGG + chr2_3:62494242-62494261 None:intergenic 40.0%
AATACCTTTGCTTTGACGAG+AGG + chr2_3:62494346-62494365 None:intergenic 40.0%
!! TGAGAAATCGAGTGTCGAAA+TGG - chr2_3:62494410-62494429 Msa0305070:three_prime_UTR 40.0%
GTTAACAATAAAGTCGCGGT+AGG + chr2_3:62494781-62494800 None:intergenic 40.0%
ATTTCGTGCTTGAATCTGTC+GGG + chr2_3:62494940-62494959 None:intergenic 40.0%
TGCACGACATGTTCAATCAA+AGG - chr2_3:62494960-62494979 Msa0305070:intron 40.0%
ATCCACATGCAGAACTGATT+TGG - chr2_3:62495068-62495087 Msa0305070:intron 40.0%
ATCCAAATCAGTTCTGCATG+TGG + chr2_3:62495073-62495092 None:intergenic 40.0%
CACAACAAGACACTGTTATG+CGG + chr2_3:62495104-62495123 None:intergenic 40.0%
AGCCATTCCGGATTTCAAAA+AGG - chr2_3:62495352-62495371 Msa0305070:intron 40.0%
GTTAGGAGAAGAAACTTGGA+AGG + chr2_3:62495380-62495399 None:intergenic 40.0%
! CTAAAGTGGCAGATTTGGTT+AGG + chr2_3:62495397-62495416 None:intergenic 40.0%
!! ATCTGCCACTTTAGCAATAC+AGG - chr2_3:62495403-62495422 Msa0305070:intron 40.0%
TCCATATCGAGATGGTTACT+CGG - chr2_3:62495462-62495481 Msa0305070:intron 40.0%
GTTACTCGGCAACTAGTTAA+TGG - chr2_3:62495476-62495495 Msa0305070:intron 40.0%
TCATGGCTTGAAAGGTGATA+TGG + chr2_3:62495511-62495530 None:intergenic 40.0%
ATGTTCCTTGTCAGCATTCA+TGG + chr2_3:62495528-62495547 None:intergenic 40.0%
TGTAAGCAAATTAGTGGGAG+GGG + chr2_3:62495787-62495806 None:intergenic 40.0%
TGAAGAAAATGTGGGTGGAA+TGG + chr2_3:62496186-62496205 None:intergenic 40.0%
! CAAGTTGAAGAAAATGTGGG+TGG + chr2_3:62496191-62496210 None:intergenic 40.0%
! GCGCAAGTTGAAGAAAATGT+GGG + chr2_3:62496194-62496213 None:intergenic 40.0%
! AGCGCAAGTTGAAGAAAATG+TGG + chr2_3:62496195-62496214 None:intergenic 40.0%
AGTTGAAACGGAGAAAGACA+GGG + chr2_3:62496292-62496311 None:intergenic 40.0%
! GAGGAAGCTATGAGTTGAAA+CGG + chr2_3:62496304-62496323 None:intergenic 40.0%
AGTAGAATTGCCTGTTTGTG+TGG - chr2_3:62496345-62496364 Msa0305070:intron 40.0%
GAGTAACCTTTGTCCAGATA+TGG - chr2_3:62496461-62496480 Msa0305070:intron 40.0%
AAGTCACCATATCTGGACAA+AGG + chr2_3:62496470-62496489 None:intergenic 40.0%
GAGACAAAAGTCACCATATC+TGG + chr2_3:62496477-62496496 None:intergenic 40.0%
TCTTCAATGGGAAAACTCCA+TGG + chr2_3:62496545-62496564 None:intergenic 40.0%
! CTGTTCGTCTTGTTCTTCAA+TGG + chr2_3:62496558-62496577 None:intergenic 40.0%
AAGAACAAGACGAACAGTAC+AGG - chr2_3:62496561-62496580 Msa0305070:intron 40.0%
TAAAGTAGCTGAGGGCAAAA+TGG + chr2_3:62496647-62496666 None:intergenic 40.0%
!!! GATTGCGCTTTGTTTTGTTC+AGG - chr2_3:62496899-62496918 Msa0305070:CDS 40.0%
!! TTAGGGGAATTTTTAGAGGG+AGG - chr2_3:62497530-62497549 Msa0305070:intron 40.0%
GGATGATGATCCAGTTATCA+TGG - chr2_3:62497718-62497737 Msa0305070:intron 40.0%
TCTTTCACGTCCATGATAAC+TGG + chr2_3:62497731-62497750 None:intergenic 40.0%
GTTATCATGGACGTGAAAGA+TGG - chr2_3:62497731-62497750 Msa0305070:intron 40.0%
ACAGTTGAGGAAGCCAAATA+TGG - chr2_3:62497787-62497806 Msa0305070:intron 40.0%
CAGTTGAGGAAGCCAAATAT+GGG - chr2_3:62497788-62497807 Msa0305070:intron 40.0%
GTCATGGCAAAACAAGATGA+AGG - chr2_3:62497815-62497834 Msa0305070:intron 40.0%
CCCCCTAAATATCTTTACTC+AGG + chr2_3:62497863-62497882 None:intergenic 40.0%
! TGGTGGTGAAATCAGTTAAG+AGG - chr2_3:62497886-62497905 Msa0305070:CDS 40.0%
! GTCACTGTTTTACGACTTAG+AGG - chr2_3:62498005-62498024 Msa0305070:five_prime_UTR 40.0%
! TCGGGCTTAATCAACCTTTT+TGG - chr2_3:62498052-62498071 Msa0305070:five_prime_UTR 40.0%
CCCAATTAAACGAGCCAAAA+AGG + chr2_3:62498069-62498088 None:intergenic 40.0%
GGAGCGATAGAAAACGAACA+AGG + chr2_3:62494285-62494304 None:intergenic 45.0%
! TTTTCTGCGAATTGGGGCTT+AGG - chr2_3:62494382-62494401 Msa0305070:three_prime_UTR 45.0%
TTTCTGCGAATTGGGGCTTA+GGG - chr2_3:62494383-62494402 Msa0305070:three_prime_UTR 45.0%
GTAGCAGAAGACGAAGAAGA+AGG + chr2_3:62494480-62494499 None:intergenic 45.0%
AGCATATGGACGATATCTGC+TGG + chr2_3:62494526-62494545 None:intergenic 45.0%
GATATCGTCCATATGCTCGA+AGG - chr2_3:62494529-62494548 Msa0305070:CDS 45.0%
AACAAAACTCACTCACCAGG+TGG + chr2_3:62494558-62494577 None:intergenic 45.0%
TTGACTAATCTAGCTGCAGG+AGG + chr2_3:62494834-62494853 None:intergenic 45.0%
AGCTTGACTAATCTAGCTGC+AGG + chr2_3:62494837-62494856 None:intergenic 45.0%
! TGCAGCTAGATTAGTCAAGC+TGG - chr2_3:62494836-62494855 Msa0305070:intron 45.0%
! GCAGCTAGATTAGTCAAGCT+GGG - chr2_3:62494837-62494856 Msa0305070:intron 45.0%
AACAAGACACTGTTATGCGG+TGG + chr2_3:62495101-62495120 None:intergenic 45.0%
TTGATGCCAAACATTTGCCC+AGG - chr2_3:62495306-62495325 Msa0305070:intron 45.0%
ATAGCTTCTCCGCCAAATCT+AGG + chr2_3:62495332-62495351 None:intergenic 45.0%
CCATCTCGATATGGACGAAT+TGG + chr2_3:62495457-62495476 None:intergenic 45.0%
CCAATTCGTCCATATCGAGA+TGG - chr2_3:62495454-62495473 Msa0305070:CDS 45.0%
GTCAGCATTCATGGCTTGAA+AGG + chr2_3:62495519-62495538 None:intergenic 45.0%
TCAAGCCATGAATGCTGACA+AGG - chr2_3:62495520-62495539 Msa0305070:intron 45.0%
GGATGAAAGAGAGCAGTACT+TGG - chr2_3:62495609-62495628 Msa0305070:intron 45.0%
CTAAAAGCCCCGTGATAAAG+GGG + chr2_3:62496036-62496055 None:intergenic 45.0%
GCTAAAAGCCCCGTGATAAA+GGG + chr2_3:62496037-62496056 None:intergenic 45.0%
GAGTTGAAACGGAGAAAGAC+AGG + chr2_3:62496293-62496312 None:intergenic 45.0%
ATTCTACTACCACCAACGAG+TGG + chr2_3:62496333-62496352 None:intergenic 45.0%
ACGGTCCATTGTTTGCAGAT+GGG + chr2_3:62496378-62496397 None:intergenic 45.0%
AACGGTCCATTGTTTGCAGA+TGG + chr2_3:62496379-62496398 None:intergenic 45.0%
AACAGTACAGGCTTGATGCT+TGG - chr2_3:62496573-62496592 Msa0305070:intron 45.0%
ACAGTACAGGCTTGATGCTT+GGG - chr2_3:62496574-62496593 Msa0305070:intron 45.0%
GTGTGTTTGAACCACGGATT+TGG + chr2_3:62496996-62497015 None:intergenic 45.0%
GTGTGTGTGTGTTTGAACCA+CGG + chr2_3:62497002-62497021 None:intergenic 45.0%
CTGTCGAGCGGTATTCATAA+GGG + chr2_3:62497771-62497790 None:intergenic 45.0%
ACTGTCGAGCGGTATTCATA+AGG + chr2_3:62497772-62497791 None:intergenic 45.0%
GCCAAATATGGGATCTGTCA+TGG - chr2_3:62497799-62497818 Msa0305070:intron 45.0%
GCCATGACAGATCCCATATT+TGG + chr2_3:62497803-62497822 None:intergenic 45.0%
AGATATTTAGGGGGAAGTGG+TGG - chr2_3:62497869-62497888 Msa0305070:CDS 45.0%
GGTTCCTCTCGTCAAAGCAA+AGG - chr2_3:62494339-62494358 Msa0305070:three_prime_UTR 50.0%
TTCTGCTACTCCTACCTGCT+CGG - chr2_3:62494491-62494510 Msa0305070:three_prime_UTR 50.0%
CAAGCTGGGTTTCGATGATC+AGG - chr2_3:62494851-62494870 Msa0305070:intron 50.0%
TCTAGGCCTGGGCAAATGTT+TGG + chr2_3:62495315-62495334 None:intergenic 50.0%
CATTTGCCCAGGCCTAGATT+TGG - chr2_3:62495317-62495336 Msa0305070:intron 50.0%
CGGAGAAGCTATAGCCATTC+CGG - chr2_3:62495340-62495359 Msa0305070:intron 50.0%
GCCGAGTAACCATCTCGATA+TGG + chr2_3:62495466-62495485 None:intergenic 50.0%
GGCTAAAAGCCCCGTGATAA+AGG + chr2_3:62496038-62496057 None:intergenic 50.0%
TATTGCCGCCAACTGTTCAG+TGG + chr2_3:62496234-62496253 None:intergenic 50.0%
GGCGGCAATATTAGAGTTGC+TGG - chr2_3:62496244-62496263 Msa0305070:intron 50.0%
TTCCTCTCCATTCCACTCGT+TGG - chr2_3:62496318-62496337 Msa0305070:intron 50.0%
ACTACCACCAACGAGTGGAA+TGG + chr2_3:62496328-62496347 None:intergenic 50.0%
ATTGTTTGCAGATGGGGGGA+AGG + chr2_3:62496371-62496390 None:intergenic 50.0%
TTCCCCCCATCTGCAAACAA+TGG - chr2_3:62496370-62496389 Msa0305070:intron 50.0%
GTCCATTGTTTGCAGATGGG+GGG + chr2_3:62496375-62496394 None:intergenic 50.0%
GGTCCATTGTTTGCAGATGG+GGG + chr2_3:62496376-62496395 None:intergenic 50.0%
CGGTCCATTGTTTGCAGATG+GGG + chr2_3:62496377-62496396 None:intergenic 50.0%
! TGGGAAATGAATGATGGGGC+AGG - chr2_3:62496420-62496439 Msa0305070:intron 50.0%
AAACTCCATGGCTGAGTAGG+AGG + chr2_3:62496533-62496552 None:intergenic 50.0%
GGAAAACTCCATGGCTGAGT+AGG + chr2_3:62496536-62496555 None:intergenic 50.0%
AAAAGGGGGACAAGGCTAGT+GGG - chr2_3:62496705-62496724 Msa0305070:intron 50.0%
TCTCATGCAGCTGGTAAGTG+AGG - chr2_3:62496933-62496952 Msa0305070:CDS 50.0%
TGAATACCGCTCGACAGTTG+AGG - chr2_3:62497774-62497793 Msa0305070:intron 50.0%
GGACGGTGAAGAACGAACGA+GGG + chr2_3:62494306-62494325 None:intergenic 55.0%
AGGACGGTGAAGAACGAACG+AGG + chr2_3:62494307-62494326 None:intergenic 55.0%
TTCTTCACCGTCCTGTCCTG+CGG - chr2_3:62494313-62494332 Msa0305070:three_prime_UTR 55.0%
AACCAAACCGCAGGACAGGA+CGG + chr2_3:62494323-62494342 None:intergenic 55.0%
GGTGGTGACCTTCGAGCATA+TGG + chr2_3:62494540-62494559 None:intergenic 55.0%
TATGCTCGAAGGTCACCACC+TGG - chr2_3:62494540-62494559 Msa0305070:CDS 55.0%
AATCTAGCTGCAGGAGGCGA+TGG + chr2_3:62494828-62494847 None:intergenic 55.0%
TCGTGCTTGAATCTGTCGGG+CGG + chr2_3:62494937-62494956 None:intergenic 55.0%
TTGCCCAGGCCTAGATTTGG+CGG - chr2_3:62495320-62495339 Msa0305070:intron 55.0%
TCTCCGCCAAATCTAGGCCT+GGG + chr2_3:62495326-62495345 None:intergenic 55.0%
TTCTCCGCCAAATCTAGGCC+TGG + chr2_3:62495327-62495346 None:intergenic 55.0%
CACCAACGAGTGGAATGGAG+AGG + chr2_3:62496323-62496342 None:intergenic 55.0%
CTCTCCATTCCACTCGTTGG+TGG - chr2_3:62496321-62496340 Msa0305070:intron 55.0%
GTCCAACGAAAAGGGGGACA+AGG - chr2_3:62496697-62496716 Msa0305070:intron 55.0%
!! AGCCTTGTCCCCCTTTTCGT+TGG + chr2_3:62496702-62496721 None:intergenic 55.0%
GAAAAGGGGGACAAGGCTAG+TGG - chr2_3:62496704-62496723 Msa0305070:intron 55.0%
AAAGGGGGACAAGGCTAGTG+GGG - chr2_3:62496706-62496725 Msa0305070:intron 55.0%
TGGCTTCCTCAACTGTCGAG+CGG + chr2_3:62497783-62497802 None:intergenic 55.0%
GAGGAACCAAACCGCAGGAC+AGG + chr2_3:62494327-62494346 None:intergenic 60.0%
GACGAGAGGAACCAAACCGC+AGG + chr2_3:62494332-62494351 None:intergenic 60.0%
TGCTACTCCTACCTGCTCGG+AGG - chr2_3:62494494-62494513 Msa0305070:three_prime_UTR 60.0%
GCTTTCACCTCCGAGCAGGT+AGG + chr2_3:62494504-62494523 None:intergenic 60.0%
GCTGCAGGAGGCGATGGATA+TGG + chr2_3:62494822-62494841 None:intergenic 60.0%
CGTGCTTGAATCTGTCGGGC+GGG + chr2_3:62494936-62494955 None:intergenic 60.0%
CAACTGTTCAGTGGCCGGTG+GGG + chr2_3:62496225-62496244 None:intergenic 60.0%
CCAACTGTTCAGTGGCCGGT+GGG + chr2_3:62496226-62496245 None:intergenic 60.0%
ACCGGCCACTGAACAGTTGG+CGG - chr2_3:62496226-62496245 Msa0305070:intron 60.0%
!! CAAGGGCACTCTCATGCAGC+TGG - chr2_3:62496924-62496943 Msa0305070:CDS 60.0%
!! GATGAAGGCGCTGAAGCTGC+TGG - chr2_3:62497830-62497849 Msa0305070:intron 60.0%
CACCGTCCTGTCCTGCGGTT+TGG - chr2_3:62494318-62494337 Msa0305070:three_prime_UTR 65.0%
!! GCTGGCTTTCACCTCCGAGC+AGG + chr2_3:62494508-62494527 None:intergenic 65.0%
TTGCGCTCCACCTTCCCCAC+CGG - chr2_3:62496208-62496227 Msa0305070:intron 65.0%
TGTTCAGTGGCCGGTGGGGA+AGG + chr2_3:62496221-62496240 None:intergenic 65.0%
CCCACCGGCCACTGAACAGT+TGG - chr2_3:62496223-62496242 Msa0305070:intron 65.0%
GCCAACTGTTCAGTGGCCGG+TGG + chr2_3:62496227-62496246 None:intergenic 65.0%
GCCGCCAACTGTTCAGTGGC+CGG + chr2_3:62496230-62496249 None:intergenic 65.0%
GGGGGGAAGGCCACACAAAC+AGG + chr2_3:62496358-62496377 None:intergenic 65.0%
TGGGGCAGGTCTGCGTGAAC+AGG - chr2_3:62496434-62496453 Msa0305070:intron 65.0%
GGGGCAGGTCTGCGTGAACA+GGG - chr2_3:62496435-62496454 Msa0305070:intron 65.0%
CAGGTCTGCGTGAACAGGGC+TGG - chr2_3:62496439-62496458 Msa0305070:intron 65.0%
GGCTGCCTCCTACTCAGCCA+TGG - chr2_3:62496525-62496544 Msa0305070:intron 65.0%
TCAGTGGCCGGTGGGGAAGG+TGG + chr2_3:62496218-62496237 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 62494223 62498171 62494223 ID=Msa0305070;Name=Msa0305070
chr2_3 mRNA 62494223 62498171 62494223 ID=Msa0305070-mRNA-1;Parent=Msa0305070;Name=Msa0305070-mRNA-1;_AED=0.06;_eAED=0.06;_QI=206|0.6|0.5|1|0.6|0.5|6|303|219
chr2_3 exon 62494223 62494678 62494223 ID=Msa0305070-mRNA-1:exon:20875;Parent=Msa0305070-mRNA-1
chr2_3 exon 62495449 62495473 62495449 ID=Msa0305070-mRNA-1:exon:20874;Parent=Msa0305070-mRNA-1
chr2_3 exon 62496764 62496798 62496764 ID=Msa0305070-mRNA-1:exon:20873;Parent=Msa0305070-mRNA-1
chr2_3 exon 62496829 62497040 62496829 ID=Msa0305070-mRNA-1:exon:20872;Parent=Msa0305070-mRNA-1
chr2_3 exon 62497536 62497637 62497536 ID=Msa0305070-mRNA-1:exon:20871;Parent=Msa0305070-mRNA-1
chr2_3 exon 62497833 62498171 62497833 ID=Msa0305070-mRNA-1:exon:20870;Parent=Msa0305070-mRNA-1
chr2_3 five_prime_UTR 62497966 62498171 62497966 ID=Msa0305070-mRNA-1:five_prime_utr;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62497833 62497965 62497833 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62497536 62497637 62497536 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62496829 62497040 62496829 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62496764 62496798 62496764 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62495449 62495473 62495449 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 CDS 62494526 62494678 62494526 ID=Msa0305070-mRNA-1:cds;Parent=Msa0305070-mRNA-1
chr2_3 three_prime_UTR 62494223 62494525 62494223 ID=Msa0305070-mRNA-1:three_prime_utr;Parent=Msa0305070-mRNA-1
Gene Sequence

>Msa0305070

ATGGAATTGAACAAACCCTCAGACTTACTTCAAAAAATGCAACATCATCCTTCTTCTTCGTCTTCTGCTACTCCTACCTGCTCGGAGGTGAAAGCCAGCAGATATCGTCCATATGCTCGAAGGTCACCACCTGATCCGAATCAAATTAGAAAATCCTACCGCGACTTTATTGTTAACGTTAATCTTGACGATATACCATATCCATCGCCTCCTGCAGCTAGATTAGTCAAGCTGGCCATTCCGGATTTCAAAAAGGATCCTTCCAAGTTTCTTCTCCTAACCAAATCTGCCACTTTAGCAATACAGGATTATAATTCACAAACTCTCACAGACTACCAATTCGTCCATATCGAGATGGTTACTCGGCAACTAGTTAATGGTATCCTTTACCATATCACCTTTCAAGCCATGAATGCTGACAAGGAACATGCAACTTTCGAAGCCACAGTTAAGAGAATCAGGATGAAAGAGAGCAGTACTTGGTACAAGGGCACTCTCATGCAGCTGGAGGATGATGATCCAGTTATCATGGACGTGAAAGATGGTAACGACGTTTATGCCCTTATGAATACCGCTCGACAGTTGAGGAAGCCAAATATGGGATCTGTCATGGCAAAACAAGATGAAGGCGCTGAAGCTGCTGGAGCATTGCCTGAGTAA
Protein sequence

>Msa0305070

MELNKPSDLLQKMQHHPSSSSSATPTCSEVKASRYRPYARRSPPDPNQIRKSYRDFIVNVNLDDIPYPSPPAARLVKLAIPDFKKDPSKFLLLTKSATLAIQDYNSQTLTDYQFVHIEMVTRQLVNGILYHITFQAMNADKEHATFEATVKRIRMKESSTWYKGTLMQLEDDDPVIMDVKDGNDVYALMNTARQLRKPNMGSVMAKQDEGAEAAGALPE*