AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0309660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0309660 MtrunA17_Chr2g0310031 77.500 40 9 0 35 74 1 40 8.08e-14 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTGGTTTGGGTGGTTT+TGG 0.230890 2_4:+4283326 Msa0309660:CDS
TGTGAAGGGGAAATTGAAAA+TGG 0.255315 2_4:-4281114 None:intergenic
CTTCTATTATAAAATTCTAT+TGG 0.326814 2_4:-4280774 None:intergenic
GGAAGTTGAACAACATGATT+TGG 0.342622 2_4:-4280996 None:intergenic
TATGAGAAAATGAAATCATC+TGG 0.370579 2_4:-4281056 None:intergenic
GATGGTGGATCAGGACTTAA+AGG 0.386606 2_4:+4281310 Msa0309660:exon
AAAAGAGAGAGAGGAAGCTT+AGG 0.416538 2_4:-4281139 None:intergenic
GATTTGGAGGAGGGATGGCC+AGG 0.418743 2_4:-4280980 None:intergenic
AAAGAACGTGTGAGAAGAAA+AGG 0.426784 2_4:-4280881 None:intergenic
GCAGTTGTTTCTGTGTTGGC+AGG 0.431100 2_4:+4283390 Msa0309660:CDS
AAGAGAGAAGGGTGAAGTGA+TGG 0.434258 2_4:-4281017 None:intergenic
AAGGTTGCAACTGCAGCAAC+TGG 0.439191 2_4:+4281329 Msa0309660:CDS
AAGGAACGAGATGGTGGATC+AGG 0.449652 2_4:+4281301 Msa0309660:exon
ATAAAGATTAAAATAACATA+GGG 0.471562 2_4:+4283486 Msa0309660:three_prime_UTR
CCTTCTACAAAATCATCTCC+TGG 0.475701 2_4:+4280962 Msa0309660:five_prime_UTR
GTCAGCAGTTGTTTCTGTGT+TGG 0.484412 2_4:+4283386 Msa0309660:CDS
GTGAAGGGGAAATTGAAAAT+GGG 0.489201 2_4:-4281113 None:intergenic
AGGAAGCTTAGGTTTGTGAA+GGG 0.489230 2_4:-4281128 None:intergenic
GATAAAGATTAAAATAACAT+AGG 0.490640 2_4:+4283485 Msa0309660:three_prime_UTR
GAGGAAGCTTAGGTTTGTGA+AGG 0.493696 2_4:-4281129 None:intergenic
GGAATGGGTCGGGTGGGAGA+AGG 0.509024 2_4:+4281282 Msa0309660:five_prime_UTR
TGTGCAGGTTGTCAGATTCA+AGG 0.512612 2_4:+4281261 Msa0309660:intron
GATTAAAATAACATAGTGGC+AGG 0.527318 2_4:+4283534 Msa0309660:three_prime_UTR
AATGAAATCATCTGGGCGGC+CGG 0.529988 2_4:-4281048 None:intergenic
GGTTGTCAGATTCAAGGAAT+GGG 0.533310 2_4:+4281267 Msa0309660:intron
TCAGATTCAAGGAATGGGTC+GGG 0.536876 2_4:+4281272 Msa0309660:five_prime_UTR
AGGTTGTCAGATTCAAGGAA+TGG 0.541574 2_4:+4281266 Msa0309660:intron
TGAACAACATGATTTGGAGG+AGG 0.549111 2_4:-4280990 None:intergenic
TCATGAAATGATTGTACATG+AGG 0.549175 2_4:+4283623 Msa0309660:three_prime_UTR
CCGGTGGTTTGTAAGAGAGA+AGG 0.557897 2_4:-4281029 None:intergenic
CGGTGGTTTGTAAGAGAGAA+GGG 0.559341 2_4:-4281028 None:intergenic
AGTTGAACAACATGATTTGG+AGG 0.559781 2_4:-4280993 None:intergenic
GTCAGATTCAAGGAATGGGT+CGG 0.562371 2_4:+4281271 Msa0309660:five_prime_UTR
AACATGATTTGGAGGAGGGA+TGG 0.566449 2_4:-4280985 None:intergenic
GATTCAAGGAATGGGTCGGG+TGG 0.567627 2_4:+4281275 Msa0309660:five_prime_UTR
ATTCAAGGAATGGGTCGGGT+GGG 0.567684 2_4:+4281276 Msa0309660:five_prime_UTR
CAAAACCTTAAAAGAGAGAG+AGG 0.569364 2_4:-4281148 None:intergenic
AATTCTATTGGTTAGTTGTG+TGG 0.569496 2_4:-4280762 None:intergenic
GGAAGCTTAGGTTTGTGAAG+GGG 0.571555 2_4:-4281127 None:intergenic
TGGGAGAAGGAACGAGATGG+TGG 0.575216 2_4:+4281295 Msa0309660:exon
GAACAACATGATTTGGAGGA+GGG 0.576552 2_4:-4280989 None:intergenic
ATGAGAAAATGAAATCATCT+GGG 0.580135 2_4:-4281055 None:intergenic
TGCTGACAGCAGCATACTCA+CGG 0.581365 2_4:-4283370 None:intergenic
CAATTGTATAAGTGTATGAG+TGG 0.604418 2_4:+4283674 Msa0309660:three_prime_UTR
TAAAGATTAAAATAACATAG+TGG 0.607047 2_4:+4283530 Msa0309660:three_prime_UTR
GAAATCATCTGGGCGGCCGG+TGG 0.610698 2_4:-4281045 None:intergenic
GAAAATTGATAGCAGAAGCA+TGG 0.616936 2_4:+4280804 Msa0309660:five_prime_UTR
CTGAAGGATCTTCAAACCGA+AGG 0.628044 2_4:+4281487 Msa0309660:CDS
GGGTGGGAGAAGGAACGAGA+TGG 0.629943 2_4:+4281292 Msa0309660:exon
CCTTCTCTCTTACAAACCAC+CGG 0.633471 2_4:+4281029 Msa0309660:five_prime_UTR
AACGTGTGAGAAGAAAAGGT+AGG 0.665735 2_4:-4280877 None:intergenic
CTACAACTTCTCCACTACCA+AGG 0.672388 2_4:+4281368 Msa0309660:CDS
TAAAGATTAAAATAACATAG+GGG 0.699413 2_4:+4283487 Msa0309660:three_prime_UTR
AGAAAATGAAATCATCTGGG+CGG 0.715630 2_4:-4281052 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAATGTATAAATAAA+GGG - chr2_4:4282897-4282916 None:intergenic 10.0%
!! TAAGAAAAATGTATAAATAA+AGG - chr2_4:4282898-4282917 None:intergenic 10.0%
!! ATAAAGATTAAAATAACATA+GGG + chr2_4:4283486-4283505 Msa0309660:three_prime_UTR 10.0%
!! CTTCTATTATAAAATTCTAT+TGG - chr2_4:4280777-4280796 None:intergenic 15.0%
!!! GTAGTTTAATCAAAATTTTA+AGG - chr2_4:4281896-4281915 None:intergenic 15.0%
!!! CATGGTTTAATAATTTTTTT+TGG + chr2_4:4282089-4282108 Msa0309660:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr2_4:4282833-4282852 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG - chr2_4:4282896-4282915 None:intergenic 15.0%
!! GATAAAGATTAAAATAACAT+AGG + chr2_4:4283485-4283504 Msa0309660:three_prime_UTR 15.0%
!! TAAAGATTAAAATAACATAG+GGG + chr2_4:4283487-4283506 Msa0309660:three_prime_UTR 15.0%
!! TAAAGATTAAAATAACATAG+TGG + chr2_4:4283487-4283506 Msa0309660:three_prime_UTR 15.0%
!!! ATTTTATAATAGAAGCATGT+TGG + chr2_4:4280782-4280801 Msa0309660:five_prime_UTR 20.0%
!!! TTGTAATGAGTTTGTTTTAT+TGG + chr2_4:4281456-4281475 Msa0309660:intron 20.0%
!!! AATGAGTTTTCTGAATTTAA+TGG + chr2_4:4281580-4281599 Msa0309660:intron 20.0%
!!! ATTGAGTTTATTTTGTTTGT+TGG + chr2_4:4281624-4281643 Msa0309660:intron 20.0%
!!! ATTTTTGTTTCTGATTCTTT+TGG + chr2_4:4281669-4281688 Msa0309660:intron 20.0%
!!! TTTCTTCAGATTTGATAATT+TGG + chr2_4:4282059-4282078 Msa0309660:intron 20.0%
!! GATGAATAAAAAATACCAAA+AGG - chr2_4:4282230-4282249 None:intergenic 20.0%
!!! GATTTTATATTCTTTGCTTT+TGG + chr2_4:4282315-4282334 Msa0309660:intron 20.0%
!!! TAAAATATTTGTTACCACAT+AGG - chr2_4:4282445-4282464 None:intergenic 20.0%
!!! TAAAATATTTGTTACCACAT+AGG - chr2_4:4282445-4282464 None:intergenic 20.0%
!!! CTTTTTAACTCATTTGAATT+TGG + chr2_4:4282665-4282684 Msa0309660:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr2_4:4282763-4282782 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr2_4:4282873-4282892 None:intergenic 20.0%
!! AGCTCATTGTAATTAAATAA+TGG + chr2_4:4283015-4283034 Msa0309660:intron 20.0%
!! GCTCATTGTAATTAAATAAT+GGG + chr2_4:4283016-4283035 Msa0309660:intron 20.0%
!!! CATTTTTTTTATCAGCTGAA+TGG - chr2_4:4280835-4280854 None:intergenic 25.0%
! CATTCAGCTGATAAAAAAAA+TGG + chr2_4:4280833-4280852 Msa0309660:five_prime_UTR 25.0%
!! ATGATTTTGTAGAAGGAAAA+AGG - chr2_4:4280958-4280977 None:intergenic 25.0%
! ATGAGAAAATGAAATCATCT+GGG - chr2_4:4281058-4281077 None:intergenic 25.0%
! TATGAGAAAATGAAATCATC+TGG - chr2_4:4281059-4281078 None:intergenic 25.0%
! AAAACTAACACAACATACAA+AGG - chr2_4:4281918-4281937 None:intergenic 25.0%
! TGATAATTTGGTCATTTACA+TGG + chr2_4:4282071-4282090 Msa0309660:intron 25.0%
! TAAATTTCAGTCAACATAAC+TGG - chr2_4:4282172-4282191 None:intergenic 25.0%
!! AGTTATGTTGACTGAAATTT+AGG + chr2_4:4282171-4282190 Msa0309660:intron 25.0%
!!! AACTTTAGAATGATACCTTT+TGG + chr2_4:4282212-4282231 Msa0309660:intron 25.0%
!! TTTTTATTCATCATGTGCAT+AGG + chr2_4:4282238-4282257 Msa0309660:intron 25.0%
! TAAAATCCTAAAAGCAAGAA+AGG - chr2_4:4282302-4282321 None:intergenic 25.0%
! ATCTATCATTATGCAACAAA+AGG - chr2_4:4282415-4282434 None:intergenic 25.0%
! TTGTTGCATAATGATAGATA+TGG + chr2_4:4282416-4282435 Msa0309660:intron 25.0%
! TGTTGCATAATGATAGATAT+GGG + chr2_4:4282417-4282436 Msa0309660:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr2_4:4282764-4282783 None:intergenic 25.0%
!!! TGAAATGAGTCAGTTATTTT+GGG - chr2_4:4282785-4282804 None:intergenic 25.0%
!! TTGAAATGAGTCAGTTATTT+TGG - chr2_4:4282786-4282805 None:intergenic 25.0%
! GAAAGTGGAATATATTGTAT+TGG - chr2_4:4282874-4282893 None:intergenic 25.0%
!!! ACATGTTTAGTACTTTTTGT+TGG + chr2_4:4283046-4283065 Msa0309660:intron 25.0%
! AAATGATTGTTTGTGATATC+TGG + chr2_4:4283084-4283103 Msa0309660:intron 25.0%
! AGAACAGTTATAATGACATA+TGG + chr2_4:4283135-4283154 Msa0309660:intron 25.0%
! TTAAGATACAATTTCCTGAT+TGG - chr2_4:4283186-4283205 None:intergenic 25.0%
! ATTTTGTAGAAGGAAAAAGG+AGG - chr2_4:4280955-4280974 None:intergenic 30.0%
CACAATCATATTCAACAAAG+AGG - chr2_4:4281245-4281264 None:intergenic 30.0%
TTTGTTGAATATGATTGTGC+AGG + chr2_4:4281246-4281265 Msa0309660:intron 30.0%
TGAAAGGAAAAATCTTACCT+TGG - chr2_4:4281388-4281407 None:intergenic 30.0%
! TTTCCTTTCAATTCTTTTGC+TGG + chr2_4:4281398-4281417 Msa0309660:intron 30.0%
! ATGAGTATAGAGTAGTGTTT+TGG + chr2_4:4282119-4282138 Msa0309660:intron 30.0%
!! ATCATTCTAAAGTTCCAGAA+TGG - chr2_4:4282206-4282225 None:intergenic 30.0%
ATCTATCATTATGCAGCAAA+AGG - chr2_4:4282480-4282499 None:intergenic 30.0%
TGTATAAATAAAGGGGAAAG+TGG - chr2_4:4282889-4282908 None:intergenic 30.0%
! GTTTTGTGAATGTTTCTTTG+AGG + chr2_4:4283213-4283232 Msa0309660:intron 30.0%
GATTAAAATAACATAGTGGC+AGG + chr2_4:4283534-4283553 Msa0309660:three_prime_UTR 30.0%
TCATGAAATGATTGTACATG+AGG + chr2_4:4283623-4283642 Msa0309660:three_prime_UTR 30.0%
! GAAAATTGATAGCAGAAGCA+TGG + chr2_4:4280804-4280823 Msa0309660:five_prime_UTR 35.0%
AAAGAACGTGTGAGAAGAAA+AGG - chr2_4:4280884-4280903 None:intergenic 35.0%
! AGTTGAACAACATGATTTGG+AGG - chr2_4:4280996-4281015 None:intergenic 35.0%
GGAAGTTGAACAACATGATT+TGG - chr2_4:4280999-4281018 None:intergenic 35.0%
AGAAAATGAAATCATCTGGG+CGG - chr2_4:4281055-4281074 None:intergenic 35.0%
GTGAAGGGGAAATTGAAAAT+GGG - chr2_4:4281116-4281135 None:intergenic 35.0%
TGTGAAGGGGAAATTGAAAA+TGG - chr2_4:4281117-4281136 None:intergenic 35.0%
CAAAACCTTAAAAGAGAGAG+AGG - chr2_4:4281151-4281170 None:intergenic 35.0%
GAAAAATCTTACCTTGGTAG+TGG - chr2_4:4281382-4281401 None:intergenic 35.0%
!!! TTTATTGGTTTTGCAGCTGA+AGG + chr2_4:4281471-4281490 Msa0309660:intron 35.0%
!!! CTCACACTGATTTTAACCTT+CGG - chr2_4:4281506-4281525 None:intergenic 35.0%
TCATTCATGTGATCTTTGTG+TGG + chr2_4:4281964-4281983 Msa0309660:intron 35.0%
CATATGAGAGTTGTTGTCAT+AGG + chr2_4:4282020-4282039 Msa0309660:intron 35.0%
!!! AGAGTAGTGTTTTGGTTGAT+TGG + chr2_4:4282127-4282146 Msa0309660:intron 35.0%
TAAAAGCAAGAAAGGAGGTA+TGG - chr2_4:4282294-4282313 None:intergenic 35.0%
!! ATACCTCCTTTCTTGCTTTT+AGG + chr2_4:4282293-4282312 Msa0309660:intron 35.0%
AATCCTAAAAGCAAGAAAGG+AGG - chr2_4:4282299-4282318 None:intergenic 35.0%
ATGCAACAAAAGGAGAAGAA+AGG - chr2_4:4282405-4282424 None:intergenic 35.0%
AATGATAGATGTGACCTATG+TGG + chr2_4:4282490-4282509 Msa0309660:intron 35.0%
GCAGTAGTGATAATAATGAC+AGG - chr2_4:4282621-4282640 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG - chr2_4:4282750-4282769 None:intergenic 35.0%
AGCCAAGAGGTCAAATAAAT+TGG + chr2_4:4282932-4282951 Msa0309660:intron 35.0%
GCCAAGAGGTCAAATAAATT+GGG + chr2_4:4282933-4282952 Msa0309660:intron 35.0%
TCCCAATTTATTTGACCTCT+TGG - chr2_4:4282937-4282956 None:intergenic 35.0%
AGAGGTCAAATAAATTGGGA+CGG + chr2_4:4282937-4282956 Msa0309660:intron 35.0%
TAGAGAGTTGTAATGACACA+TGG + chr2_4:4283109-4283128 Msa0309660:intron 35.0%
!!! GTGTTTTAATGACTCCAATC+AGG + chr2_4:4283169-4283188 Msa0309660:intron 35.0%
ATATGCTGTCAGTATCTGTT+TGG + chr2_4:4283287-4283306 Msa0309660:intron 35.0%
!!! TCTGTTTTTTTGGCTGGTTT+GGG + chr2_4:4283317-4283336 Msa0309660:CDS 35.0%
AACGTGTGAGAAGAAAAGGT+AGG - chr2_4:4280880-4280899 None:intergenic 40.0%
GTAGAAGGAAAAAGGAGGAA+AGG - chr2_4:4280950-4280969 None:intergenic 40.0%
! CCAGGAGATGATTTTGTAGA+AGG - chr2_4:4280965-4280984 None:intergenic 40.0%
CCTTCTACAAAATCATCTCC+TGG + chr2_4:4280962-4280981 Msa0309660:five_prime_UTR 40.0%
! GAACAACATGATTTGGAGGA+GGG - chr2_4:4280992-4281011 None:intergenic 40.0%
! TGAACAACATGATTTGGAGG+AGG - chr2_4:4280993-4281012 None:intergenic 40.0%
AGGAAGCTTAGGTTTGTGAA+GGG - chr2_4:4281131-4281150 None:intergenic 40.0%
AAAAGAGAGAGAGGAAGCTT+AGG - chr2_4:4281142-4281161 None:intergenic 40.0%
! AGCTTCCTCTCTCTCTTTTA+AGG + chr2_4:4281143-4281162 Msa0309660:five_prime_UTR 40.0%
AGGTTGTCAGATTCAAGGAA+TGG + chr2_4:4281266-4281285 Msa0309660:intron 40.0%
GGTTGTCAGATTCAAGGAAT+GGG + chr2_4:4281267-4281286 Msa0309660:intron 40.0%
TCGCCAGCAAAAGAATTGAA+AGG - chr2_4:4281404-4281423 None:intergenic 40.0%
TCAGAAAACTCATTCGATCG+CGG - chr2_4:4281574-4281593 None:intergenic 40.0%
! TTTGTGTGGTTGACTTCTAC+AGG + chr2_4:4281978-4281997 Msa0309660:intron 40.0%
TTAGGCTCTTGAATCCATTC+TGG + chr2_4:4282189-4282208 Msa0309660:intron 40.0%
AAAGGAGAAGAAAGGAGGTA+TGG - chr2_4:4282397-4282416 None:intergenic 40.0%
CAACAAAAGGAGAAGAAAGG+AGG - chr2_4:4282402-4282421 None:intergenic 40.0%
TAGTTTGGATGGCAACTTCA+AGG - chr2_4:4282569-4282588 None:intergenic 40.0%
TTGCCATCCAAACTATGTGA+AGG + chr2_4:4282574-4282593 Msa0309660:intron 40.0%
ACACCTTCACATAGTTTGGA+TGG - chr2_4:4282580-4282599 None:intergenic 40.0%
ATGCACACCTTCACATAGTT+TGG - chr2_4:4282584-4282603 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG - chr2_4:4282749-4282768 None:intergenic 40.0%
GTCAAATAAATTGGGACGGA+GGG + chr2_4:4282941-4282960 Msa0309660:intron 40.0%
!! ACTAAGCTTTAAGCAGAGCT+TGG - chr2_4:4282979-4282998 None:intergenic 40.0%
GCTAACAAACTGAGAAGCTT+TGG - chr2_4:4283259-4283278 None:intergenic 40.0%
!!! TGGTTGCAGGTCTGTTTTTT+TGG + chr2_4:4283307-4283326 Msa0309660:intron 40.0%
!!! GTCTGTTTTTTTGGCTGGTT+TGG + chr2_4:4283316-4283335 Msa0309660:CDS 40.0%
! AACATGATTTGGAGGAGGGA+TGG - chr2_4:4280988-4281007 None:intergenic 45.0%
AAGAGAGAAGGGTGAAGTGA+TGG - chr2_4:4281020-4281039 None:intergenic 45.0%
! CGGTGGTTTGTAAGAGAGAA+GGG - chr2_4:4281031-4281050 None:intergenic 45.0%
CCTTCTCTCTTACAAACCAC+CGG + chr2_4:4281029-4281048 Msa0309660:five_prime_UTR 45.0%
GGAAGCTTAGGTTTGTGAAG+GGG - chr2_4:4281130-4281149 None:intergenic 45.0%
GAGGAAGCTTAGGTTTGTGA+AGG - chr2_4:4281132-4281151 None:intergenic 45.0%
TGTGCAGGTTGTCAGATTCA+AGG + chr2_4:4281261-4281280 Msa0309660:intron 45.0%
GTCAGATTCAAGGAATGGGT+CGG + chr2_4:4281271-4281290 Msa0309660:five_prime_UTR 45.0%
TCAGATTCAAGGAATGGGTC+GGG + chr2_4:4281272-4281291 Msa0309660:five_prime_UTR 45.0%
!! GATGGTGGATCAGGACTTAA+AGG + chr2_4:4281310-4281329 Msa0309660:exon 45.0%
CTACAACTTCTCCACTACCA+AGG + chr2_4:4281368-4281387 Msa0309660:CDS 45.0%
! TTTTGCTGGCGAGTAACTTG+CGG + chr2_4:4281412-4281431 Msa0309660:intron 45.0%
CTGAAGGATCTTCAAACCGA+AGG + chr2_4:4281487-4281506 Msa0309660:CDS 45.0%
! TTAAGGCTGAAAGCACACAC+TGG - chr2_4:4281879-4281898 None:intergenic 45.0%
GATAGATATGGGCACCTATG+TGG + chr2_4:4282428-4282447 Msa0309660:intron 45.0%
TCTGTGTGAAATGAGCCAAG+AGG + chr2_4:4282919-4282938 Msa0309660:intron 45.0%
GGTCAAATAAATTGGGACGG+AGG + chr2_4:4282940-4282959 Msa0309660:intron 45.0%
GTCAGTATCTGTTTGGTTGC+AGG + chr2_4:4283294-4283313 Msa0309660:intron 45.0%
!!! TGCAGGTCTGTTTTTTTGGC+TGG + chr2_4:4283311-4283330 Msa0309660:intron 45.0%
!!! GTTTTTTTGGCTGGTTTGGG+TGG + chr2_4:4283320-4283339 Msa0309660:CDS 45.0%
!!! TTGGGTGGTTTTGGCTGTTT+TGG + chr2_4:4283335-4283354 Msa0309660:CDS 45.0%
!! GTCAGCAGTTGTTTCTGTGT+TGG + chr2_4:4283386-4283405 Msa0309660:CDS 45.0%
!! AATATTTAAAAAATATAAAG+TGG - chr2_4:4282643-4282662 None:intergenic 5.0%
! CCGGTGGTTTGTAAGAGAGA+AGG - chr2_4:4281032-4281051 None:intergenic 50.0%
AATGAAATCATCTGGGCGGC+CGG - chr2_4:4281051-4281070 None:intergenic 50.0%
ATTCAAGGAATGGGTCGGGT+GGG + chr2_4:4281276-4281295 Msa0309660:five_prime_UTR 50.0%
!! AAGGAACGAGATGGTGGATC+AGG + chr2_4:4281301-4281320 Msa0309660:exon 50.0%
AAGGTTGCAACTGCAGCAAC+TGG + chr2_4:4281329-4281348 Msa0309660:CDS 50.0%
ATGGGTCAGTTATTCCGGGA+CGG - chr2_4:4282745-4282764 None:intergenic 50.0%
!!! TTGGCTGGTTTGGGTGGTTT+TGG + chr2_4:4283326-4283345 Msa0309660:CDS 50.0%
TGCTGACAGCAGCATACTCA+CGG - chr2_4:4283373-4283392 None:intergenic 50.0%
!! GCAGTTGTTTCTGTGTTGGC+AGG + chr2_4:4283390-4283409 Msa0309660:CDS 50.0%
GATTCAAGGAATGGGTCGGG+TGG + chr2_4:4281275-4281294 Msa0309660:five_prime_UTR 55.0%
TGGGAGAAGGAACGAGATGG+TGG + chr2_4:4281295-4281314 Msa0309660:exon 55.0%
GATTTCTACTCCCTCCGTCC+CGG + chr2_4:4282728-4282747 Msa0309660:intron 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr2_4:4282741-4282760 None:intergenic 55.0%
GATTTGGAGGAGGGATGGCC+AGG - chr2_4:4280983-4281002 None:intergenic 60.0%
GGGTGGGAGAAGGAACGAGA+TGG + chr2_4:4281292-4281311 Msa0309660:exon 60.0%
GGTCAGTTATTCCGGGACGG+AGG - chr2_4:4282742-4282761 None:intergenic 60.0%
GAAATCATCTGGGCGGCCGG+TGG - chr2_4:4281048-4281067 None:intergenic 65.0%
GGAATGGGTCGGGTGGGAGA+AGG + chr2_4:4281282-4281301 Msa0309660:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 4280765 4283695 4280765 ID=Msa0309660;Name=Msa0309660
chr2_4 mRNA 4280765 4283695 4280765 ID=Msa0309660-mRNA-1;Parent=Msa0309660;Name=Msa0309660-mRNA-1;_AED=0.13;_eAED=0.13;_QI=443|0.66|1|1|0.66|0.75|4|268|74
chr2_4 exon 4280765 4281164 4280765 ID=Msa0309660-mRNA-1:exon:1442;Parent=Msa0309660-mRNA-1
chr2_4 exon 4281268 4281389 4281268 ID=Msa0309660-mRNA-1:exon:1443;Parent=Msa0309660-mRNA-1
chr2_4 exon 4281487 4281520 4281487 ID=Msa0309660-mRNA-1:exon:1444;Parent=Msa0309660-mRNA-1
chr2_4 exon 4283316 4283695 4283316 ID=Msa0309660-mRNA-1:exon:1445;Parent=Msa0309660-mRNA-1
chr2_4 five_prime_UTR 4280765 4281164 4280765 ID=Msa0309660-mRNA-1:five_prime_utr;Parent=Msa0309660-mRNA-1
chr2_4 five_prime_UTR 4281268 4281310 4281268 ID=Msa0309660-mRNA-1:five_prime_utr;Parent=Msa0309660-mRNA-1
chr2_4 CDS 4281311 4281389 4281311 ID=Msa0309660-mRNA-1:cds;Parent=Msa0309660-mRNA-1
chr2_4 CDS 4281487 4281520 4281487 ID=Msa0309660-mRNA-1:cds;Parent=Msa0309660-mRNA-1
chr2_4 CDS 4283316 4283427 4283316 ID=Msa0309660-mRNA-1:cds;Parent=Msa0309660-mRNA-1
chr2_4 three_prime_UTR 4283428 4283695 4283428 ID=Msa0309660-mRNA-1:three_prime_utr;Parent=Msa0309660-mRNA-1
Gene Sequence

>Msa0309660

ATGGTGGATCAGGACTTAAAGGTTGCAACTGCAGCAACTGGTTGTTTTTCATATGTTCTACAACTTCTCCACTACCAAGCTGAAGGATCTTCAAACCGAAGGTTAAAATCAGTGTCTGTTTTTTTGGCTGGTTTGGGTGGTTTTGGCTGTTTTGGTGCAGCTGTTAGCCGTGAGTATGCTGCTGTCAGCAGTTGTTTCTGTGTTGGCAGGCTGTTGTCATCATAA
Protein sequence

>Msa0309660

MVDQDLKVATAATGCFSYVLQLLHYQAEGSSNRRLKSVSVFLAGLGGFGCFGAAVSREYAAVSSCFCVGRLLSS*