AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0394280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0394280 MtrunA17_Chr2g0310031 86.111 36 5 0 81 116 5 40 5.10e-13 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGCTGGTTTGGGTGGTTT+TGG 0.288579 3_2:-8856556 Msa0394280:CDS
GATGGTGGATCAGGACTTAA+AGG 0.386606 3_2:-8858392 Msa0394280:CDS
AAAGAACGTGTGAGAAGAAA+AGG 0.426784 3_2:+8858821 None:intergenic
GCAGTTGTTTCTGTGTTGGC+AGG 0.431100 3_2:-8856492 Msa0394280:CDS
AAGGTTGCAACTGCAGCAAC+TGG 0.439191 3_2:-8858373 Msa0394280:CDS
AAGGAACGAGATGGTGGATC+AGG 0.449652 3_2:-8858401 Msa0394280:CDS
ATAAAGATTAAAATAACATA+GGG 0.471562 3_2:-8856396 Msa0394280:three_prime_UTR
GTCAGCAGTTGTTTCTGTGT+TGG 0.484412 3_2:-8856496 Msa0394280:CDS
GATAAAGATTAAAATAACAT+AGG 0.490640 3_2:-8856397 Msa0394280:three_prime_UTR
GGAATGGGTCGGGTGGGAGA+AGG 0.509024 3_2:-8858420 Msa0394280:CDS
TGTGCAGGTTGTCAGATTCA+AGG 0.512612 3_2:-8858441 Msa0394280:intron
GATTAAAATAACATAGTGGC+AGG 0.527318 3_2:-8856348 Msa0394280:three_prime_UTR
GGTTGTCAGATTCAAGGAAT+GGG 0.533310 3_2:-8858435 Msa0394280:CDS
TCAGATTCAAGGAATGGGTC+GGG 0.536876 3_2:-8858430 Msa0394280:CDS
AGGTTGTCAGATTCAAGGAA+TGG 0.541574 3_2:-8858436 Msa0394280:CDS
TCATGAAATGATTGTACATG+AGG 0.549175 3_2:-8856259 Msa0394280:three_prime_UTR
GTCAGATTCAAGGAATGGGT+CGG 0.562371 3_2:-8858431 Msa0394280:CDS
GATTCAAGGAATGGGTCGGG+TGG 0.567627 3_2:-8858427 Msa0394280:CDS
ATTCAAGGAATGGGTCGGGT+GGG 0.567684 3_2:-8858426 Msa0394280:CDS
TGGGAGAAGGAACGAGATGG+TGG 0.575216 3_2:-8858407 Msa0394280:CDS
TGCTGACAGCAGCATACTCA+CGG 0.581365 3_2:+8856512 None:intergenic
CAATTGTATAAGTGTATGAG+TGG 0.604418 3_2:-8856208 None:intergenic
TAAAGATTAAAATAACATAG+TGG 0.607047 3_2:-8856352 Msa0394280:three_prime_UTR
CTGAAGGATCTTCAAACCGA+AGG 0.628044 3_2:-8858215 Msa0394280:CDS
GGGTGGGAGAAGGAACGAGA+TGG 0.629943 3_2:-8858410 Msa0394280:CDS
AACGTGTGAGAAGAAAAGGT+AGG 0.665735 3_2:+8858825 None:intergenic
CTACAACTTCTCCACTACCA+AGG 0.672388 3_2:-8858334 Msa0394280:intron
TAAAGATTAAAATAACATAG+GGG 0.699413 3_2:-8856395 Msa0394280:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTTAAAAAATATAAAG+TGG + chr3_2:8858052-8858071 None:intergenic 10.0%
!! ATAAAGATTAAAATAACATA+GGG - chr3_2:8858663-8858682 Msa0394280:intron 10.0%
!!! GTAGTTTAATCAAAATTTTA+AGG + chr3_2:8857253-8857272 None:intergenic 15.0%
!!! CATGGTTTAATAATTTTTTT+TGG - chr3_2:8857446-8857465 Msa0394280:intron 15.0%
!! GATAAAGATTAAAATAACAT+AGG - chr3_2:8858662-8858681 Msa0394280:intron 15.0%
!! TAAAGATTAAAATAACATAG+GGG - chr3_2:8858664-8858683 Msa0394280:intron 15.0%
!! TAAAGATTAAAATAACATAG+TGG - chr3_2:8858664-8858683 Msa0394280:intron 15.0%
!!! TTGTAATGAGTTTGTTTTAT+TGG - chr3_2:8856813-8856832 Msa0394280:intron 20.0%
!!! AATGAGTTTTCTGAATTTAA+TGG - chr3_2:8856937-8856956 Msa0394280:intron 20.0%
!!! ATTGAGTTTATTTTGTTTGT+TGG - chr3_2:8856981-8857000 Msa0394280:intron 20.0%
!!! TTTCTTCAGATTTGATAATT+TGG - chr3_2:8857416-8857435 Msa0394280:intron 20.0%
!! GATGAATAAAAAATACCAAA+AGG + chr3_2:8857587-8857606 None:intergenic 20.0%
!!! GATTTTATATTCTTTGCTTT+TGG - chr3_2:8857672-8857691 Msa0394280:intron 20.0%
!!! TAAAATATTTGTTACCACAT+AGG + chr3_2:8857802-8857821 None:intergenic 20.0%
!!! TAAAATATTTGTTACCACAT+AGG + chr3_2:8857802-8857821 None:intergenic 20.0%
!!! CTTTTTAACTCATTTGAATT+TGG - chr3_2:8858074-8858093 Msa0394280:intron 20.0%
!! AGCTCATTGTAATTAAATAA+TGG - chr3_2:8858192-8858211 Msa0394280:intron 20.0%
!! GCTCATTGTAATTAAATAAT+GGG - chr3_2:8858193-8858212 Msa0394280:intron 20.0%
!! ATGATTTTGTAGAAGGAAAA+AGG + chr3_2:8856315-8856334 None:intergenic 25.0%
! ATGAGAAAATGAAATCATCT+GGG + chr3_2:8856415-8856434 None:intergenic 25.0%
! TATGAGAAAATGAAATCATC+TGG + chr3_2:8856416-8856435 None:intergenic 25.0%
!! ATTTTTGTGTCTGATTCTTT+TGG - chr3_2:8857026-8857045 Msa0394280:intron 25.0%
! AAAACTAACACAACATACAA+AGG + chr3_2:8857275-8857294 None:intergenic 25.0%
! TGATAATTTGGTCATTTACA+TGG - chr3_2:8857428-8857447 Msa0394280:intron 25.0%
! TAAATTTCAGTCAACATAAC+TGG + chr3_2:8857529-8857548 None:intergenic 25.0%
!! AGTTATGTTGACTGAAATTT+AGG - chr3_2:8857528-8857547 Msa0394280:intron 25.0%
!!! AACTTTAGAATGATACCTTT+TGG - chr3_2:8857569-8857588 Msa0394280:intron 25.0%
!! TTTTTATTCATCATGTGCAT+AGG - chr3_2:8857595-8857614 Msa0394280:intron 25.0%
! TAAAATCCTAAAAGCAAGAA+AGG + chr3_2:8857659-8857678 None:intergenic 25.0%
! ATCTATCATTATGCAACAAA+AGG + chr3_2:8857772-8857791 None:intergenic 25.0%
! TTGTTGCATAATGATAGATA+TGG - chr3_2:8857773-8857792 Msa0394280:intron 25.0%
! TGTTGCATAATGATAGATAT+GGG - chr3_2:8857774-8857793 Msa0394280:intron 25.0%
! AAATGATTGTTTGTGATATC+TGG - chr3_2:8858261-8858280 Msa0394280:intron 25.0%
! AGAACAGTTATAATGACATA+TGG - chr3_2:8858312-8858331 Msa0394280:intron 25.0%
! TTAAGATACAATTTCCTGAT+TGG + chr3_2:8858363-8858382 None:intergenic 25.0%
! ATTTTGTAGAAGGAAAAAGG+AGG + chr3_2:8856312-8856331 None:intergenic 30.0%
CACAATCATATTCAACAAAG+AGG + chr3_2:8856602-8856621 None:intergenic 30.0%
TTTGTTGAATATGATTGTGC+AGG - chr3_2:8856603-8856622 Msa0394280:intron 30.0%
TGAAAGGAAAAATCTTACCT+TGG + chr3_2:8856745-8856764 None:intergenic 30.0%
! TTTCCTTTCAATTCTTTTGC+TGG - chr3_2:8856755-8856774 Msa0394280:intron 30.0%
! ATGAGTATAGAGTAGTGTTT+TGG - chr3_2:8857476-8857495 Msa0394280:intron 30.0%
!! ATCATTCTAAAGTTCCAGAA+TGG + chr3_2:8857563-8857582 None:intergenic 30.0%
ATCTATCATTATGCAGCAAA+AGG + chr3_2:8857837-8857856 None:intergenic 30.0%
AATGATAGATATGACCTATG+TGG - chr3_2:8857847-8857866 Msa0394280:intron 30.0%
ACATGTTTAGTACTTTGTGT+TGG - chr3_2:8858223-8858242 Msa0394280:intron 30.0%
! GTTTTGTGAATGTTTCTTTG+AGG - chr3_2:8858390-8858409 Msa0394280:CDS 30.0%
!!! TCTGTTTTTTTAGCTGGTTT+GGG - chr3_2:8858494-8858513 Msa0394280:intron 30.0%
GATTAAAATAACATAGTGGC+AGG - chr3_2:8858711-8858730 Msa0394280:intron 30.0%
TCATGAAATGATTGTACATG+AGG - chr3_2:8858800-8858819 Msa0394280:CDS 30.0%
AAAGAACGTGTGAGAAGAAA+AGG + chr3_2:8856241-8856260 None:intergenic 35.0%
AGAAAATGAAATCATCTGGG+CGG + chr3_2:8856412-8856431 None:intergenic 35.0%
GTGAAGGGGAAATTGAAAAT+GGG + chr3_2:8856473-8856492 None:intergenic 35.0%
TGTGAAGGGGAAATTGAAAA+TGG + chr3_2:8856474-8856493 None:intergenic 35.0%
CAAAACCTTAAAAGAGAGAG+AGG + chr3_2:8856508-8856527 None:intergenic 35.0%
GAAAAATCTTACCTTGGTAG+TGG + chr3_2:8856739-8856758 None:intergenic 35.0%
!!! TTTATTGGTTTTGCAGCTGA+AGG - chr3_2:8856828-8856847 Msa0394280:intron 35.0%
!! CTCACTCTGATTTTAACCTT+CGG + chr3_2:8856863-8856882 None:intergenic 35.0%
TCATTCATGTGATCTTTGTG+TGG - chr3_2:8857321-8857340 Msa0394280:intron 35.0%
CATATGAGAGTTGTTGTCAT+AGG - chr3_2:8857377-8857396 Msa0394280:intron 35.0%
!!! AGAGTAGTGTTTTGGTTGAT+TGG - chr3_2:8857484-8857503 Msa0394280:intron 35.0%
TAAAAGCAAGAAAGGAGGTA+TGG + chr3_2:8857651-8857670 None:intergenic 35.0%
!! ATACCTCCTTTCTTGCTTTT+AGG - chr3_2:8857650-8857669 Msa0394280:intron 35.0%
AATCCTAAAAGCAAGAAAGG+AGG + chr3_2:8857656-8857675 None:intergenic 35.0%
ATGCAACAAAAGGAGAAGAA+AGG + chr3_2:8857762-8857781 None:intergenic 35.0%
GCAGTAGTGATAATAATGAC+AGG + chr3_2:8858030-8858049 None:intergenic 35.0%
TAGAGAGTTGTAATGACACA+TGG - chr3_2:8858286-8858305 Msa0394280:intron 35.0%
!!! GTGTTTTAATGACTCCAATC+AGG - chr3_2:8858346-8858365 Msa0394280:CDS 35.0%
ATATGCTGTCAGTATCTGTT+TGG - chr3_2:8858464-8858483 Msa0394280:intron 35.0%
!!! GTCTGTTTTTTTAGCTGGTT+TGG - chr3_2:8858493-8858512 Msa0394280:intron 35.0%
AACGTGTGAGAAGAAAAGGT+AGG + chr3_2:8856237-8856256 None:intergenic 40.0%
GTAGAAGGAAAAAGGAGGAA+AGG + chr3_2:8856307-8856326 None:intergenic 40.0%
! CCAGGAGATGATTTTGTAGA+AGG + chr3_2:8856322-8856341 None:intergenic 40.0%
CCTTCTACAAAATCATCTCC+TGG - chr3_2:8856319-8856338 Msa0394280:three_prime_UTR 40.0%
AGTTGAACAACAGGATTTGG+AGG + chr3_2:8856353-8856372 None:intergenic 40.0%
GGAAGTTGAACAACAGGATT+TGG + chr3_2:8856356-8856375 None:intergenic 40.0%
! AGTGATGGAAGTTGAACAAC+AGG + chr3_2:8856362-8856381 None:intergenic 40.0%
AGGAAGCTTAGGTTTGTGAA+GGG + chr3_2:8856488-8856507 None:intergenic 40.0%
AAAAGAGAGAGAGGAAGCTT+AGG + chr3_2:8856499-8856518 None:intergenic 40.0%
! AGCTTCCTCTCTCTCTTTTA+AGG - chr3_2:8856500-8856519 Msa0394280:CDS 40.0%
AGGTTGTCAGATTCAAGGAA+TGG - chr3_2:8856623-8856642 Msa0394280:intron 40.0%
GGTTGTCAGATTCAAGGAAT+GGG - chr3_2:8856624-8856643 Msa0394280:intron 40.0%
TCGCCAGCAAAAGAATTGAA+AGG + chr3_2:8856761-8856780 None:intergenic 40.0%
TCAGAAAACTCATTCGATCG+CGG + chr3_2:8856931-8856950 None:intergenic 40.0%
! TTAAGGTTGAAAGCACACAC+TGG + chr3_2:8857236-8857255 None:intergenic 40.0%
! TTTGTGTGGTTGACTTCTAC+AGG - chr3_2:8857335-8857354 Msa0394280:intron 40.0%
TTAGGCTCTTGAATCCATTC+TGG - chr3_2:8857546-8857565 Msa0394280:intron 40.0%
AAAGGAGAAGAAAGGAGGTA+TGG + chr3_2:8857754-8857773 None:intergenic 40.0%
CAACAAAAGGAGAAGAAAGG+AGG + chr3_2:8857759-8857778 None:intergenic 40.0%
TAGTTTGGATGGCAACTTCA+AGG + chr3_2:8857926-8857945 None:intergenic 40.0%
TTGCCATCCAAACTATGTGA+AGG - chr3_2:8857931-8857950 Msa0394280:intron 40.0%
ACACCTTCACATAGTTTGGA+TGG + chr3_2:8857937-8857956 None:intergenic 40.0%
ATGCACACCTTCACATAGTT+TGG + chr3_2:8857941-8857960 None:intergenic 40.0%
TAGTTTGGATGGCATCTTCA+AGG + chr3_2:8857978-8857997 None:intergenic 40.0%
ATGCCATCCAAACTATGTGA+AGG - chr3_2:8857983-8858002 Msa0394280:intron 40.0%
ACACCTTCACATAGTTTGGA+TGG + chr3_2:8857937-8857956 None:intergenic 40.0%
ATGCACACCTTCACATAGTT+TGG + chr3_2:8857941-8857960 None:intergenic 40.0%
!! ACTAAGCTTTAAGCAGAGCT+TGG + chr3_2:8858156-8858175 None:intergenic 40.0%
!!! TGCAGGTCTGTTTTTTTAGC+TGG - chr3_2:8858488-8858507 Msa0394280:intron 40.0%
!!! GTTTTTTTAGCTGGTTTGGG+TGG - chr3_2:8858497-8858516 Msa0394280:intron 40.0%
GAACAACAGGATTTGGAGGA+GGG + chr3_2:8856349-8856368 None:intergenic 45.0%
TGAACAACAGGATTTGGAGG+AGG + chr3_2:8856350-8856369 None:intergenic 45.0%
AAGAGAGAAGGGTGAAGTGA+TGG + chr3_2:8856377-8856396 None:intergenic 45.0%
! CGGTGGTTTGTAAGAGAGAA+GGG + chr3_2:8856388-8856407 None:intergenic 45.0%
CCTTCTCTCTTACAAACCAC+CGG - chr3_2:8856386-8856405 Msa0394280:three_prime_UTR 45.0%
GGAAGCTTAGGTTTGTGAAG+GGG + chr3_2:8856487-8856506 None:intergenic 45.0%
GAGGAAGCTTAGGTTTGTGA+AGG + chr3_2:8856489-8856508 None:intergenic 45.0%
TGTGCAGGTTGTCAGATTCA+AGG - chr3_2:8856618-8856637 Msa0394280:intron 45.0%
GTCAGATTCAAGGAATGGGT+CGG - chr3_2:8856628-8856647 Msa0394280:intron 45.0%
TCAGATTCAAGGAATGGGTC+GGG - chr3_2:8856629-8856648 Msa0394280:intron 45.0%
!! GATGGTGGATCAGGACTTAA+AGG - chr3_2:8856667-8856686 Msa0394280:intron 45.0%
CTACAACTTCTCCACTACCA+AGG - chr3_2:8856725-8856744 Msa0394280:intron 45.0%
! TTTTGCTGGCGAGTAACTTG+CGG - chr3_2:8856769-8856788 Msa0394280:intron 45.0%
CTGAAGGATCTTCAAACCGA+AGG - chr3_2:8856844-8856863 Msa0394280:intron 45.0%
GATAGATATGGGCACCTATG+TGG - chr3_2:8857785-8857804 Msa0394280:intron 45.0%
GTCAGTATCTGTTTGGTTGC+AGG - chr3_2:8858471-8858490 Msa0394280:intron 45.0%
!!! TTAGCTGGTTTGGGTGGTTT+TGG - chr3_2:8858503-8858522 Msa0394280:intron 45.0%
!!! TTGGGTGGTTTTGGCTGTTT+TGG - chr3_2:8858512-8858531 Msa0394280:intron 45.0%
!! GTCAGCAGTTGTTTCTGTGT+TGG - chr3_2:8858563-8858582 Msa0394280:intron 45.0%
AACAGGATTTGGAGGAGGGA+TGG + chr3_2:8856345-8856364 None:intergenic 50.0%
! CCGGTGGTTTGTAAGAGAGA+AGG + chr3_2:8856389-8856408 None:intergenic 50.0%
AATGAAATCATCTGGGCGGC+CGG + chr3_2:8856408-8856427 None:intergenic 50.0%
ATTCAAGGAATGGGTCGGGT+GGG - chr3_2:8856633-8856652 Msa0394280:intron 50.0%
!! AAGGAACGAGATGGTGGATC+AGG - chr3_2:8856658-8856677 Msa0394280:intron 50.0%
AAGGTTGCAACTGCAGCAAC+TGG - chr3_2:8856686-8856705 Msa0394280:intron 50.0%
TGCTGACAGCAGCATACTCA+CGG + chr3_2:8858550-8858569 None:intergenic 50.0%
!! GCAGTTGTTTCTGTGTTGGC+AGG - chr3_2:8858567-8858586 Msa0394280:intron 50.0%
GATTCAAGGAATGGGTCGGG+TGG - chr3_2:8856632-8856651 Msa0394280:intron 55.0%
TGGGAGAAGGAACGAGATGG+TGG - chr3_2:8856652-8856671 Msa0394280:intron 55.0%
GATTTGGAGGAGGGATGGCC+AGG + chr3_2:8856340-8856359 None:intergenic 60.0%
GGGTGGGAGAAGGAACGAGA+TGG - chr3_2:8856649-8856668 Msa0394280:intron 60.0%
GAAATCATCTGGGCGGCCGG+TGG + chr3_2:8856405-8856424 None:intergenic 65.0%
GGAATGGGTCGGGTGGGAGA+AGG - chr3_2:8856639-8856658 Msa0394280:intron 65.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 8856209 8858872 8856209 ID=Msa0394280;Name=Msa0394280
chr3_2 mRNA 8856209 8858872 8856209 ID=Msa0394280-mRNA-1;Parent=Msa0394280;Name=Msa0394280-mRNA-1;_AED=0.26;_eAED=0.36;_QI=0|0.33|0.75|1|1|1|4|268|116
chr3_2 exon 8856209 8856588 8856209 ID=Msa0394280-mRNA-1:exon:2323;Parent=Msa0394280-mRNA-1
chr3_2 exon 8858204 8858237 8858204 ID=Msa0394280-mRNA-1:exon:2322;Parent=Msa0394280-mRNA-1
chr3_2 exon 8858335 8858456 8858335 ID=Msa0394280-mRNA-1:exon:2321;Parent=Msa0394280-mRNA-1
chr3_2 exon 8858790 8858872 8858790 ID=Msa0394280-mRNA-1:exon:2320;Parent=Msa0394280-mRNA-1
chr3_2 CDS 8858790 8858872 8858790 ID=Msa0394280-mRNA-1:cds;Parent=Msa0394280-mRNA-1
chr3_2 CDS 8858335 8858456 8858335 ID=Msa0394280-mRNA-1:cds;Parent=Msa0394280-mRNA-1
chr3_2 CDS 8858204 8858237 8858204 ID=Msa0394280-mRNA-1:cds;Parent=Msa0394280-mRNA-1
chr3_2 CDS 8856477 8856588 8856477 ID=Msa0394280-mRNA-1:cds;Parent=Msa0394280-mRNA-1
chr3_2 three_prime_UTR 8856209 8856476 8856209 ID=Msa0394280-mRNA-1:three_prime_utr;Parent=Msa0394280-mRNA-1
Gene Sequence

>Msa0394280

ATGGAATGTGCACAAATCACAAAATCCTACCTTTTCTTCTCACACGTTCTTTCTGATTCAGAATTCAGAAAAAACAGCTTGCTGTTGTCAGATTCAAGGAATGGGTCGGGTGGGAGAAGGAACGAGATGGTGGATCAGGACTTAAAGGTTGCAACTGCAGCAACTGGTTGTTTTTCATATGTTCTACAACTTCTCCACTACCAAGCTGAAGGATCTTCAAACCGAAGGTTAAAATCAGAGTCTGTTTTTTTAGCTGGTTTGGGTGGTTTTGGCTGTTTTGGTGCAGCTGTTAGCCGTGAGTATGCTGCTGTCAGCAGTTGTTTCTGTGTTGGCAGGCTGTTGTCATCATAA

Protein sequence

>Msa0394280

MECAQITKSYLFFSHVLSDSEFRKNSLLLSDSRNGSGGRRNEMVDQDLKVATAATGCFSYVLQLLHYQAEGSSNRRLKSESVFLAGLGGFGCFGAAVSREYAAVSSCFCVGRLLSS*