AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0311940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTATAAATACATGATTTC+TGG 0.146830 2_4:+6921341 Msa0311940:five_prime_UTR
GACATTAATTATACATAATT+AGG 0.147476 2_4:-6922691 None:intergenic
TTGTAATTTATGTCTGCTTT+TGG 0.220362 2_4:+6922656 Msa0311940:three_prime_UTR
TTATACATAATTAGGGATTT+AGG 0.243430 2_4:-6922683 None:intergenic
TATTATACTCCAGCTGATTA+TGG 0.249358 2_4:+6922467 Msa0311940:three_prime_UTR
AAATCACTGATCTGCTAAAA+AGG 0.252867 2_4:-6921681 None:intergenic
CTCTAGTAGTTTGCGGCTTT+GGG 0.258315 2_4:+6922613 Msa0311940:three_prime_UTR
TCTCTAGTAGTTTGCGGCTT+TGG 0.263573 2_4:+6922612 Msa0311940:three_prime_UTR
ACATAAATTACAATACTAAT+AGG 0.269901 2_4:-6922646 None:intergenic
TCAAAGCTTTGACAACAGTA+TGG 0.296747 2_4:+6922111 Msa0311940:CDS
TCTTTCTCTCGCCAAACGTT+CGG 0.330046 2_4:-6921946 None:intergenic
ACATTAATTATACATAATTA+GGG 0.355027 2_4:-6922690 None:intergenic
CTTTCTCTCGCCAAACGTTC+GGG 0.360603 2_4:-6921945 None:intergenic
TTAGACTTCTCCCGAACGTT+TGG 0.363687 2_4:+6921935 Msa0311940:five_prime_UTR
TCCAGCTGATTATGGATTAG+AGG 0.379886 2_4:+6922475 Msa0311940:three_prime_UTR
GACTACCTTCCAACTTAGTA+TGG 0.391394 2_4:+6922332 Msa0311940:exon
GCGCCCAGGGTGCAGCCTCT+TGG 0.396019 2_4:+6922425 Msa0311940:three_prime_UTR
AACTTATCTCTAGTAGTTTG+CGG 0.403782 2_4:+6922606 Msa0311940:three_prime_UTR
GTACTCAAATCGGTCTTCAT+AGG 0.417050 2_4:-6922366 None:intergenic
TCAACAAAGTTGTCATCAAA+TGG 0.417779 2_4:+6921876 Msa0311940:five_prime_UTR
GGGCCAAGAGGCTGCACCCT+GGG 0.431419 2_4:-6922428 None:intergenic
ATGAAGACCGATTTGAGTAC+TGG 0.440283 2_4:+6922369 Msa0311940:three_prime_UTR
CATAAATTACAATACTAATA+GGG 0.445876 2_4:-6922645 None:intergenic
TCTATAAATACATGATTTCT+GGG 0.446078 2_4:+6921342 Msa0311940:five_prime_UTR
CGATAATATGTCCAATGAAA+AGG 0.453370 2_4:+6922007 Msa0311940:CDS
CCAATCGTTGTTGTGCGCCC+AGG 0.455468 2_4:+6922411 Msa0311940:three_prime_UTR
AATCCGTAGTAGTATTAGTA+AGG 0.481719 2_4:+6922569 Msa0311940:three_prime_UTR
TCCTCTAATCCATAATCAGC+TGG 0.484339 2_4:-6922476 None:intergenic
CAATCGTTGTTGTGCGCCCA+GGG 0.486723 2_4:+6922412 Msa0311940:three_prime_UTR
AGCGATCCCAGTACTCAAAT+CGG 0.492770 2_4:-6922376 None:intergenic
CAAAGCTTTGACAACAGTAT+GGG 0.494576 2_4:+6922112 Msa0311940:CDS
AAGCAAGCAACCTATTCTCT+TGG 0.499131 2_4:-6921400 None:intergenic
AGTAAATAACAGTTTAGAGC+AGG 0.502627 2_4:-6922715 None:intergenic
CCCTGGTGATATCATGTTCG+AGG 0.505379 2_4:+6922310 Msa0311940:CDS
CCTGGGCGCACAACAACGAT+TGG 0.508160 2_4:-6922411 None:intergenic
GATTAGAGGATGATGATTGA+TGG 0.509050 2_4:+6922489 Msa0311940:three_prime_UTR
TACAAATTATTTACCCGGGT+CGG 0.521216 2_4:-6921306 None:intergenic
TAATAACTAGCTGGACGTTG+TGG 0.523669 2_4:-6922449 None:intergenic
ACTCCTTACTAATACTACTA+CGG 0.527646 2_4:-6922572 None:intergenic
AATAACTAGCTGGACGTTGT+GGG 0.528802 2_4:-6922448 None:intergenic
TTCAAAACATGAATCAGACT+TGG 0.536182 2_4:+6922145 Msa0311940:CDS
TGGGCCAAGAGGCTGCACCC+TGG 0.540694 2_4:-6922429 None:intergenic
TTGAGCCTACCATACTAAGT+TGG 0.541664 2_4:-6922341 None:intergenic
TCCTCGAACATGATATCACC+AGG 0.543019 2_4:-6922311 None:intergenic
GCTGGAGTATAATAACTAGC+TGG 0.548205 2_4:-6922458 None:intergenic
GTATTTATAGAGCGGCCGCT+TGG 0.554556 2_4:-6921330 None:intergenic
GTTAAGAGTGCGTATGAGAA+GGG 0.563631 2_4:+6922185 Msa0311940:CDS
TGTTAAGAGTGCGTATGAGA+AGG 0.565399 2_4:+6922184 Msa0311940:CDS
TTGGTTACAAATTATTTACC+CGG 0.577076 2_4:-6921311 None:intergenic
ACAACAACGATTGGCACGCG+TGG 0.595849 2_4:-6922402 None:intergenic
AACCTCGTAACTGTTGCCAC+TGG 0.597504 2_4:-6921540 None:intergenic
CTTACATCTCACTAAAATCA+CGG 0.598432 2_4:-6922211 None:intergenic
AACTTAGTATGGTAGGCTCA+AGG 0.607295 2_4:+6922343 Msa0311940:exon
TGAAGACCGATTTGAGTACT+GGG 0.608071 2_4:+6922370 Msa0311940:three_prime_UTR
ACCTTCCAACTTAGTATGGT+AGG 0.609611 2_4:+6922336 Msa0311940:exon
GCCTACCATACTAAGTTGGA+AGG 0.612784 2_4:-6922337 None:intergenic
TGTGGTGGACATAACCGACC+CGG 0.621203 2_4:+6921292 None:intergenic
CTTGATCTTGATAATGACGT+GGG 0.622967 2_4:+6922053 Msa0311940:CDS
TTCCAGTGGCAACAGTTACG+AGG 0.632420 2_4:+6921538 Msa0311940:five_prime_UTR
GAAATCATGTATTTATAGAG+CGG 0.640417 2_4:-6921338 None:intergenic
AAAGCTTTGACAACAGTATG+GGG 0.642690 2_4:+6922113 Msa0311940:CDS
TGGTTACAAATTATTTACCC+GGG 0.649137 2_4:-6921310 None:intergenic
TCTTGATCTTGATAATGACG+TGG 0.649200 2_4:+6922052 Msa0311940:CDS
GTGGTGGACATAACCGACCC+GGG 0.650786 2_4:+6921293 None:intergenic
GCTGGACGTTGTGGGCCAAG+AGG 0.661359 2_4:-6922440 None:intergenic
GTAAATAATTTGTAACCAAG+CGG 0.664321 2_4:+6921315 Msa0311940:five_prime_UTR
AAGAGTGCGTATGAGAAGGG+CGG 0.697112 2_4:+6922188 Msa0311940:CDS
CTTCACATAATGTCTTCCAG+TGG 0.702452 2_4:+6921524 Msa0311940:five_prime_UTR
CCTCGAACATGATATCACCA+GGG 0.756060 2_4:-6922310 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGATAATAAAACATTAA+CGG - chr2_4:6922267-6922286 None:intergenic 10.0%
!! ACATTAATTATACATAATTA+GGG - chr2_4:6922693-6922712 None:intergenic 10.0%
!! ATTAGATAATAAAACATTAA+CGG - chr2_4:6922267-6922286 None:intergenic 10.0%
!! ACATTAATTATACATAATTA+GGG - chr2_4:6922693-6922712 None:intergenic 10.0%
!! AGAACATATATTAGATAAAA+GGG - chr2_4:6921470-6921489 None:intergenic 15.0%
!! AAGAACATATATTAGATAAA+AGG - chr2_4:6921471-6921490 None:intergenic 15.0%
!! CATAAATTACAATACTAATA+GGG - chr2_4:6922648-6922667 None:intergenic 15.0%
!! ACATAAATTACAATACTAAT+AGG - chr2_4:6922649-6922668 None:intergenic 15.0%
!! GACATTAATTATACATAATT+AGG - chr2_4:6922694-6922713 None:intergenic 15.0%
!! AGAACATATATTAGATAAAA+GGG - chr2_4:6921470-6921489 None:intergenic 15.0%
!! AAGAACATATATTAGATAAA+AGG - chr2_4:6921471-6921490 None:intergenic 15.0%
!! CATAAATTACAATACTAATA+GGG - chr2_4:6922648-6922667 None:intergenic 15.0%
!! ACATAAATTACAATACTAAT+AGG - chr2_4:6922649-6922668 None:intergenic 15.0%
!! GACATTAATTATACATAATT+AGG - chr2_4:6922694-6922713 None:intergenic 15.0%
!! TCTATAAATACATGATTTCT+GGG + chr2_4:6921342-6921361 Msa0311940:five_prime_UTR 20.0%
!! TTATACATAATTAGGGATTT+AGG - chr2_4:6922686-6922705 None:intergenic 20.0%
!! TCTATAAATACATGATTTCT+GGG + chr2_4:6921342-6921361 Msa0311940:five_prime_UTR 20.0%
!! TTATACATAATTAGGGATTT+AGG - chr2_4:6922686-6922705 None:intergenic 20.0%
! TTGGTTACAAATTATTTACC+CGG - chr2_4:6921314-6921333 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG + chr2_4:6921315-6921334 Msa0311940:five_prime_UTR 25.0%
! GAAATCATGTATTTATAGAG+CGG - chr2_4:6921341-6921360 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG + chr2_4:6921341-6921360 Msa0311940:five_prime_UTR 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG + chr2_4:6921390-6921409 Msa0311940:five_prime_UTR 25.0%
! CAAAAACAATAGAGAGAAAA+AGG - chr2_4:6921827-6921846 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr2_4:6921851-6921870 None:intergenic 25.0%
!! TTGTAATTTATGTCTGCTTT+TGG + chr2_4:6922656-6922675 Msa0311940:three_prime_UTR 25.0%
!!! TGTAATTTATGTCTGCTTTT+GGG + chr2_4:6922657-6922676 Msa0311940:three_prime_UTR 25.0%
! TTGGTTACAAATTATTTACC+CGG - chr2_4:6921314-6921333 None:intergenic 25.0%
!! GTAAATAATTTGTAACCAAG+CGG + chr2_4:6921315-6921334 Msa0311940:five_prime_UTR 25.0%
! GAAATCATGTATTTATAGAG+CGG - chr2_4:6921341-6921360 None:intergenic 25.0%
! CTCTATAAATACATGATTTC+TGG + chr2_4:6921341-6921360 Msa0311940:five_prime_UTR 25.0%
!!! TATTTTTTCACCAAGAGAAT+AGG + chr2_4:6921390-6921409 Msa0311940:five_prime_UTR 25.0%
! CAAAAACAATAGAGAGAAAA+AGG - chr2_4:6921827-6921846 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG - chr2_4:6921851-6921870 None:intergenic 25.0%
!! TTGTAATTTATGTCTGCTTT+TGG + chr2_4:6922656-6922675 Msa0311940:three_prime_UTR 25.0%
!!! TGTAATTTATGTCTGCTTTT+GGG + chr2_4:6922657-6922676 Msa0311940:three_prime_UTR 25.0%
TGGTTACAAATTATTTACCC+GGG - chr2_4:6921313-6921332 None:intergenic 30.0%
AAATCACTGATCTGCTAAAA+AGG - chr2_4:6921595-6921614 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr2_4:6921755-6921774 Msa0311940:five_prime_UTR 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr2_4:6921876-6921895 Msa0311940:five_prime_UTR 30.0%
CGATAATATGTCCAATGAAA+AGG + chr2_4:6922007-6922026 Msa0311940:CDS 30.0%
TTCAAAACATGAATCAGACT+TGG + chr2_4:6922145-6922164 Msa0311940:CDS 30.0%
CTTACATCTCACTAAAATCA+CGG - chr2_4:6922214-6922233 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG - chr2_4:6922294-6922313 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG + chr2_4:6922293-6922312 Msa0311940:intron 30.0%
TATTATACTCCAGCTGATTA+TGG + chr2_4:6922467-6922486 Msa0311940:three_prime_UTR 30.0%
AATCCGTAGTAGTATTAGTA+AGG + chr2_4:6922569-6922588 Msa0311940:three_prime_UTR 30.0%
ACTCCTTACTAATACTACTA+CGG - chr2_4:6922575-6922594 None:intergenic 30.0%
AACTTATCTCTAGTAGTTTG+CGG + chr2_4:6922606-6922625 Msa0311940:three_prime_UTR 30.0%
!! GTAATTTATGTCTGCTTTTG+GGG + chr2_4:6922658-6922677 Msa0311940:three_prime_UTR 30.0%
! AGTAAATAACAGTTTAGAGC+AGG - chr2_4:6922718-6922737 None:intergenic 30.0%
TGGTTACAAATTATTTACCC+GGG - chr2_4:6921313-6921332 None:intergenic 30.0%
AAATCACTGATCTGCTAAAA+AGG - chr2_4:6921595-6921614 None:intergenic 30.0%
!!! AACCACTTTTTGACAACTTT+TGG + chr2_4:6921755-6921774 Msa0311940:five_prime_UTR 30.0%
TCAACAAAGTTGTCATCAAA+TGG + chr2_4:6921876-6921895 Msa0311940:five_prime_UTR 30.0%
CGATAATATGTCCAATGAAA+AGG + chr2_4:6922007-6922026 Msa0311940:CDS 30.0%
TTCAAAACATGAATCAGACT+TGG + chr2_4:6922145-6922164 Msa0311940:CDS 30.0%
CTTACATCTCACTAAAATCA+CGG - chr2_4:6922214-6922233 None:intergenic 30.0%
AGGGTCACTGAAAATATAAT+AGG - chr2_4:6922294-6922313 None:intergenic 30.0%
! TATTATATTTTCAGTGACCC+TGG + chr2_4:6922293-6922312 Msa0311940:intron 30.0%
TATTATACTCCAGCTGATTA+TGG + chr2_4:6922467-6922486 Msa0311940:three_prime_UTR 30.0%
AATCCGTAGTAGTATTAGTA+AGG + chr2_4:6922569-6922588 Msa0311940:three_prime_UTR 30.0%
ACTCCTTACTAATACTACTA+CGG - chr2_4:6922575-6922594 None:intergenic 30.0%
AACTTATCTCTAGTAGTTTG+CGG + chr2_4:6922606-6922625 Msa0311940:three_prime_UTR 30.0%
!! GTAATTTATGTCTGCTTTTG+GGG + chr2_4:6922658-6922677 Msa0311940:three_prime_UTR 30.0%
! AGTAAATAACAGTTTAGAGC+AGG - chr2_4:6922718-6922737 None:intergenic 30.0%
TACAAATTATTTACCCGGGT+CGG - chr2_4:6921309-6921328 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr2_4:6921760-6921779 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG - chr2_4:6922021-6922040 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG + chr2_4:6922052-6922071 Msa0311940:CDS 35.0%
!! CTTGATCTTGATAATGACGT+GGG + chr2_4:6922053-6922072 Msa0311940:CDS 35.0%
! TCAAAGCTTTGACAACAGTA+TGG + chr2_4:6922111-6922130 Msa0311940:CDS 35.0%
! CAAAGCTTTGACAACAGTAT+GGG + chr2_4:6922112-6922131 Msa0311940:CDS 35.0%
! AAAGCTTTGACAACAGTATG+GGG + chr2_4:6922113-6922132 Msa0311940:CDS 35.0%
! ACGCACTCTTAACATTTTCA+AGG - chr2_4:6922178-6922197 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG + chr2_4:6922489-6922508 Msa0311940:three_prime_UTR 35.0%
!! TTGATGGAACTACTTTTCTG+AGG + chr2_4:6922505-6922524 Msa0311940:three_prime_UTR 35.0%
!! AACTACTTTTCTGAGGATGA+TGG + chr2_4:6922512-6922531 Msa0311940:three_prime_UTR 35.0%
!! TTTTTCTCTAGTTTGCGCTT+TGG + chr2_4:6922540-6922559 Msa0311940:three_prime_UTR 35.0%
TACAAATTATTTACCCGGGT+CGG - chr2_4:6921309-6921328 None:intergenic 35.0%
GTCCAAAAGTTGTCAAAAAG+TGG - chr2_4:6921760-6921779 None:intergenic 35.0%
! AATATGACGAGCCTTTTCAT+TGG - chr2_4:6922021-6922040 None:intergenic 35.0%
!! TCTTGATCTTGATAATGACG+TGG + chr2_4:6922052-6922071 Msa0311940:CDS 35.0%
!! CTTGATCTTGATAATGACGT+GGG + chr2_4:6922053-6922072 Msa0311940:CDS 35.0%
! TCAAAGCTTTGACAACAGTA+TGG + chr2_4:6922111-6922130 Msa0311940:CDS 35.0%
! CAAAGCTTTGACAACAGTAT+GGG + chr2_4:6922112-6922131 Msa0311940:CDS 35.0%
! AAAGCTTTGACAACAGTATG+GGG + chr2_4:6922113-6922132 Msa0311940:CDS 35.0%
! ACGCACTCTTAACATTTTCA+AGG - chr2_4:6922178-6922197 None:intergenic 35.0%
GATTAGAGGATGATGATTGA+TGG + chr2_4:6922489-6922508 Msa0311940:three_prime_UTR 35.0%
!! TTGATGGAACTACTTTTCTG+AGG + chr2_4:6922505-6922524 Msa0311940:three_prime_UTR 35.0%
!! AACTACTTTTCTGAGGATGA+TGG + chr2_4:6922512-6922531 Msa0311940:three_prime_UTR 35.0%
!! TTTTTCTCTAGTTTGCGCTT+TGG + chr2_4:6922540-6922559 Msa0311940:three_prime_UTR 35.0%
AAGCAAGCAACCTATTCTCT+TGG - chr2_4:6921403-6921422 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG + chr2_4:6921524-6921543 Msa0311940:five_prime_UTR 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG + chr2_4:6922184-6922203 Msa0311940:CDS 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG + chr2_4:6922185-6922204 Msa0311940:CDS 40.0%
GACTACCTTCCAACTTAGTA+TGG + chr2_4:6922332-6922351 Msa0311940:exon 40.0%
ACCTTCCAACTTAGTATGGT+AGG + chr2_4:6922336-6922355 Msa0311940:exon 40.0%
TTGAGCCTACCATACTAAGT+TGG - chr2_4:6922344-6922363 None:intergenic 40.0%
AACTTAGTATGGTAGGCTCA+AGG + chr2_4:6922343-6922362 Msa0311940:exon 40.0%
GTACTCAAATCGGTCTTCAT+AGG - chr2_4:6922369-6922388 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG + chr2_4:6922369-6922388 Msa0311940:three_prime_UTR 40.0%
!! TGAAGACCGATTTGAGTACT+GGG + chr2_4:6922370-6922389 Msa0311940:three_prime_UTR 40.0%
AATAACTAGCTGGACGTTGT+GGG - chr2_4:6922451-6922470 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG - chr2_4:6922452-6922471 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG - chr2_4:6922461-6922480 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG + chr2_4:6922475-6922494 Msa0311940:three_prime_UTR 40.0%
TCCTCTAATCCATAATCAGC+TGG - chr2_4:6922479-6922498 None:intergenic 40.0%
AAGCAAGCAACCTATTCTCT+TGG - chr2_4:6921403-6921422 None:intergenic 40.0%
CTTCACATAATGTCTTCCAG+TGG + chr2_4:6921524-6921543 Msa0311940:five_prime_UTR 40.0%
! TGTTAAGAGTGCGTATGAGA+AGG + chr2_4:6922184-6922203 Msa0311940:CDS 40.0%
! GTTAAGAGTGCGTATGAGAA+GGG + chr2_4:6922185-6922204 Msa0311940:CDS 40.0%
GACTACCTTCCAACTTAGTA+TGG + chr2_4:6922332-6922351 Msa0311940:exon 40.0%
ACCTTCCAACTTAGTATGGT+AGG + chr2_4:6922336-6922355 Msa0311940:exon 40.0%
TTGAGCCTACCATACTAAGT+TGG - chr2_4:6922344-6922363 None:intergenic 40.0%
AACTTAGTATGGTAGGCTCA+AGG + chr2_4:6922343-6922362 Msa0311940:exon 40.0%
GTACTCAAATCGGTCTTCAT+AGG - chr2_4:6922369-6922388 None:intergenic 40.0%
!! ATGAAGACCGATTTGAGTAC+TGG + chr2_4:6922369-6922388 Msa0311940:three_prime_UTR 40.0%
!! TGAAGACCGATTTGAGTACT+GGG + chr2_4:6922370-6922389 Msa0311940:three_prime_UTR 40.0%
AATAACTAGCTGGACGTTGT+GGG - chr2_4:6922451-6922470 None:intergenic 40.0%
TAATAACTAGCTGGACGTTG+TGG - chr2_4:6922452-6922471 None:intergenic 40.0%
GCTGGAGTATAATAACTAGC+TGG - chr2_4:6922461-6922480 None:intergenic 40.0%
!! TCCAGCTGATTATGGATTAG+AGG + chr2_4:6922475-6922494 Msa0311940:three_prime_UTR 40.0%
TCCTCTAATCCATAATCAGC+TGG - chr2_4:6922479-6922498 None:intergenic 40.0%
TTAGACTTCTCCCGAACGTT+TGG + chr2_4:6921935-6921954 Msa0311940:five_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG - chr2_4:6921949-6921968 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG - chr2_4:6922313-6922332 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG - chr2_4:6922314-6922333 None:intergenic 45.0%
! GCCTACCATACTAAGTTGGA+AGG - chr2_4:6922340-6922359 None:intergenic 45.0%
AGCGATCCCAGTACTCAAAT+CGG - chr2_4:6922379-6922398 None:intergenic 45.0%
! TCTCTAGTAGTTTGCGGCTT+TGG + chr2_4:6922612-6922631 Msa0311940:three_prime_UTR 45.0%
! CTCTAGTAGTTTGCGGCTTT+GGG + chr2_4:6922613-6922632 Msa0311940:three_prime_UTR 45.0%
TTAGACTTCTCCCGAACGTT+TGG + chr2_4:6921935-6921954 Msa0311940:five_prime_UTR 45.0%
TCTTTCTCTCGCCAAACGTT+CGG - chr2_4:6921949-6921968 None:intergenic 45.0%
CCTCGAACATGATATCACCA+GGG - chr2_4:6922313-6922332 None:intergenic 45.0%
TCCTCGAACATGATATCACC+AGG - chr2_4:6922314-6922333 None:intergenic 45.0%
! GCCTACCATACTAAGTTGGA+AGG - chr2_4:6922340-6922359 None:intergenic 45.0%
AGCGATCCCAGTACTCAAAT+CGG - chr2_4:6922379-6922398 None:intergenic 45.0%
! TCTCTAGTAGTTTGCGGCTT+TGG + chr2_4:6922612-6922631 Msa0311940:three_prime_UTR 45.0%
! CTCTAGTAGTTTGCGGCTTT+GGG + chr2_4:6922613-6922632 Msa0311940:three_prime_UTR 45.0%
GTATTTATAGAGCGGCCGCT+TGG - chr2_4:6921333-6921352 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG + chr2_4:6921538-6921557 Msa0311940:five_prime_UTR 50.0%
AACCTCGTAACTGTTGCCAC+TGG - chr2_4:6921543-6921562 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG - chr2_4:6921948-6921967 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG + chr2_4:6922188-6922207 Msa0311940:CDS 50.0%
CCCTGGTGATATCATGTTCG+AGG + chr2_4:6922310-6922329 Msa0311940:CDS 50.0%
GTATTTATAGAGCGGCCGCT+TGG - chr2_4:6921333-6921352 None:intergenic 50.0%
TTCCAGTGGCAACAGTTACG+AGG + chr2_4:6921538-6921557 Msa0311940:five_prime_UTR 50.0%
AACCTCGTAACTGTTGCCAC+TGG - chr2_4:6921543-6921562 None:intergenic 50.0%
CTTTCTCTCGCCAAACGTTC+GGG - chr2_4:6921948-6921967 None:intergenic 50.0%
! AAGAGTGCGTATGAGAAGGG+CGG + chr2_4:6922188-6922207 Msa0311940:CDS 50.0%
CCCTGGTGATATCATGTTCG+AGG + chr2_4:6922310-6922329 Msa0311940:CDS 50.0%
ACAACAACGATTGGCACGCG+TGG - chr2_4:6922405-6922424 None:intergenic 55.0%
CAATCGTTGTTGTGCGCCCA+GGG + chr2_4:6922412-6922431 Msa0311940:three_prime_UTR 55.0%
ACAACAACGATTGGCACGCG+TGG - chr2_4:6922405-6922424 None:intergenic 55.0%
CAATCGTTGTTGTGCGCCCA+GGG + chr2_4:6922412-6922431 Msa0311940:three_prime_UTR 55.0%
CCTGGGCGCACAACAACGAT+TGG - chr2_4:6922414-6922433 None:intergenic 60.0%
CCAATCGTTGTTGTGCGCCC+AGG + chr2_4:6922411-6922430 Msa0311940:three_prime_UTR 60.0%
CCTGGGCGCACAACAACGAT+TGG - chr2_4:6922414-6922433 None:intergenic 60.0%
CCAATCGTTGTTGTGCGCCC+AGG + chr2_4:6922411-6922430 Msa0311940:three_prime_UTR 60.0%
GCTGGACGTTGTGGGCCAAG+AGG - chr2_4:6922443-6922462 None:intergenic 65.0%
GCTGGACGTTGTGGGCCAAG+AGG - chr2_4:6922443-6922462 None:intergenic 65.0%
GGGCCAAGAGGCTGCACCCT+GGG - chr2_4:6922431-6922450 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG - chr2_4:6922432-6922451 None:intergenic 70.0%
GGGCCAAGAGGCTGCACCCT+GGG - chr2_4:6922431-6922450 None:intergenic 70.0%
TGGGCCAAGAGGCTGCACCC+TGG - chr2_4:6922432-6922451 None:intergenic 70.0%
GCGCCCAGGGTGCAGCCTCT+TGG + chr2_4:6922425-6922444 Msa0311940:three_prime_UTR 75.0%
GCGCCCAGGGTGCAGCCTCT+TGG + chr2_4:6922425-6922444 Msa0311940:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 6921304 6922764 6921304 ID=Msa0311940;Name=Msa0311940
chr2_4 mRNA 6921304 6922764 6921304 ID=Msa0311940-mRNA-1;Parent=Msa0311940;Name=Msa0311940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=592|1|1|1|0.5|0.33|3|415|89
chr2_4 exon 6921304 6921411 6921304 ID=Msa0311940-mRNA-1:exon:2646;Parent=Msa0311940-mRNA-1
chr2_4 exon 6921518 6922228 6921518 ID=Msa0311940-mRNA-1:exon:2647;Parent=Msa0311940-mRNA-1
chr2_4 exon 6922307 6922764 6922307 ID=Msa0311940-mRNA-1:exon:2648;Parent=Msa0311940-mRNA-1
chr2_4 five_prime_UTR 6921304 6921411 6921304 ID=Msa0311940-mRNA-1:five_prime_utr;Parent=Msa0311940-mRNA-1
chr2_4 five_prime_UTR 6921518 6922001 6921518 ID=Msa0311940-mRNA-1:five_prime_utr;Parent=Msa0311940-mRNA-1
chr2_4 CDS 6922002 6922228 6922002 ID=Msa0311940-mRNA-1:cds;Parent=Msa0311940-mRNA-1
chr2_4 CDS 6922307 6922349 6922307 ID=Msa0311940-mRNA-1:cds;Parent=Msa0311940-mRNA-1
chr2_4 three_prime_UTR 6922350 6922764 6922350 ID=Msa0311940-mRNA-1:three_prime_utr;Parent=Msa0311940-mRNA-1
Gene Sequence

>Msa0311940

ATGATCGATAATATGTCCAATGAAAAGGCTCGTCATATTTTTAATCTTGATCTTGATCTTGATAATGACGTGGGTTTTCATTTTGTAAAAAAATGTTACAAGAAGATTATCAAAGCTTTGACAACAGTATGGGGTGCTCGTATTTCAAAACATGAATCAGACTTGGATACACACCTTGAAAATGTTAAGAGTGCGTATGAGAAGGGCGGCCGTGATTTTAGTGAGATTGACCCTGGTGATATCATGTTCGAGGACTACCTTCCAACTTAG
Protein sequence

>Msa0311940

MIDNMSNEKARHIFNLDLDLDNDVGFHFVKKCYKKIIKALTTVWGARISKHESDLDTHLENVKSAYEKGGRDFSEIDPGDIMFEDYLPT*