AlfalfaGEDB | Msa0314540

M. sativa cultivar zhongmu-4 / Msa0314540

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF12796.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0314540	XP_013462623.1	95.329	471	8	1	1	471	1	457	0.0	926

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0314540	sp\|F4IS56\|ILK1_ARATH	45.824	443	212	5	8	436	43	471	2.62e-127	381

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0236070	Msa0314540	0.818928	1.517232e-52	-8.615850e-47
Msa0274100	Msa0314540	0.820760	5.783943e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0314540	MtrunA17_Chr2g0284901	95.329	471	8	1	1	471	1	457	0.0	926
Msa0314540	MtrunA17_Chr5g0443471	62.805	492	133	6	1	467	1	467	0.0	598
Msa0314540	MtrunA17_Chr8g0380551	45.843	445	215	5	4	436	69	499	1.32e-127	380
Msa0314540	MtrunA17_Chr4g0042731	42.961	412	202	8	42	437	22	416	3.20e-104	317
Msa0314540	MtrunA17_Chr5g0406251	34.528	307	163	11	146	443	288	565	2.33e-45	166
Msa0314540	MtrunA17_Chr1g0211611	34.098	305	141	11	146	430	294	558	1.30e-44	164
Msa0314540	MtrunA17_Chr2g0294351	36.620	284	157	9	145	425	458	721	9.01e-42	158
Msa0314540	MtrunA17_Chr3g0143531	34.307	274	150	9	160	431	38	283	1.51e-41	149
Msa0314540	MtrunA17_Chr5g0427651	33.542	319	189	8	111	425	604	903	1.37e-40	155
Msa0314540	MtrunA17_Chr1g0171451	36.237	287	157	7	142	425	408	671	1.41e-40	154

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0314540	AT4G18950.1	66.966	445	127	3	8	447	9	438	0.0	621
Msa0314540	AT4G18950.2	66.440	441	124	4	8	443	9	430	0.0	604
Msa0314540	AT3G58760.1	61.491	483	155	5	1	469	3	468	0.0	583
Msa0314540	AT3G58760.3	61.491	483	155	5	1	469	66	531	0.0	582
Msa0314540	AT3G58760.5	61.491	483	155	5	1	469	66	531	0.0	582
Msa0314540	AT3G58760.6	66.758	364	107	3	1	350	3	366	5.49e-173	491
Msa0314540	AT3G58760.2	66.758	364	107	3	1	350	66	429	3.69e-172	491
Msa0314540	AT3G58760.4	66.758	364	107	3	1	350	66	429	3.69e-172	491
Msa0314540	AT2G43850.1	45.824	443	212	5	8	436	43	471	2.66e-128	381
Msa0314540	AT2G43850.3	45.824	443	212	5	8	436	43	471	2.81e-128	381

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Find 116 sgRNAs with CRISPR-Local

Find 381 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACAATCATGAACTT+GGG	0.564897	2_4:-10303314	None:intergenic
AAACCTCAGGAGCCACATAG+CGG	0.724011	2_4:-10300803	None:intergenic
AAGCTAACAGTAATATATTG+CGG	0.591871	2_4:+10300517	Msa0314540:intron
AATAACGCTTGATTGGAGCT+CGG	0.640636	2_4:-10301796	None:intergenic
AATATATTGCGGGATGATTC+CGG	0.271441	2_4:+10300528	Msa0314540:CDS
AATCCAACAAGGTTCAAGCT+AGG	0.572761	2_4:+10297048	Msa0314540:CDS
ACAGGTTGATTGAAGAGTGC+TGG	0.486534	2_4:+10302701	Msa0314540:intron
ACCTAAAAGTTGCAGACTTT+GGG	0.219191	2_4:+10300553	Msa0314540:CDS
ACCTGCAGGTTAGACCATTG+AGG	0.664425	2_4:+10303081	Msa0314540:intron
ACGCTTGATTGGAGCTCGGA+AGG	0.520231	2_4:-10301792	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTGATCCTAAAGATCAA+TGG	+	chr2_4:10297322-10297341	Msa0314540:CDS	30.0%
TGTTGATCCTAAAGATCAAT+GGG	+	chr2_4:10297323-10297342	Msa0314540:CDS	30.0%
GACTAATTGGTTAAGATTTG+TGG	+	chr2_4:10297409-10297428	Msa0314540:intron	30.0%
GTCATTAGTGTAAGATAGAT+TGG	+	chr2_4:10297463-10297482	Msa0314540:intron	30.0%
AAGACTCTGAATTATGAGTA+TGG	+	chr2_4:10297818-10297837	Msa0314540:intron	30.0%
GGATATAGAATAAAGGGAAT+TGG	+	chr2_4:10297993-10298012	Msa0314540:intron	30.0%
TCATGAATACAATGATAGAG+AGG	+	chr2_4:10298093-10298112	Msa0314540:intron	30.0%
CATGAATACAATGATAGAGA+GGG	+	chr2_4:10298094-10298113	Msa0314540:intron	30.0%
ATGAATACAATGATAGAGAG+GGG	+	chr2_4:10298095-10298114	Msa0314540:intron	30.0%
TAAAATATAGCATCTGCAAG+GGG	-	chr2_4:10298352-10298371	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	10296839	10303527	10296839	ID=Msa0314540;Name=Msa0314540
chr2_4	mRNA	10296839	10303527	10296839	ID=Msa0314540-mRNA-1;Parent=Msa0314540;Name=Msa0314540-mRNA-1;_AED=0.11;_eAED=0.11;_QI=197\|0.83\|0.92\|0.92\|1\|1\|13\|331\|471
chr2_4	exon	10296839	10297353	10296839	ID=Msa0314540-mRNA-1:exon:3974;Parent=Msa0314540-mRNA-1
chr2_4	exon	10298349	10298423	10298349	ID=Msa0314540-mRNA-1:exon:3975;Parent=Msa0314540-mRNA-1
chr2_4	exon	10298527	10298622	10298527	ID=Msa0314540-mRNA-1:exon:3976;Parent=Msa0314540-mRNA-1
chr2_4	exon	10299110	10299195	10299110	ID=Msa0314540-mRNA-1:exon:3977;Parent=Msa0314540-mRNA-1
chr2_4	exon	10299281	10299401	10299281	ID=Msa0314540-mRNA-1:exon:3978;Parent=Msa0314540-mRNA-1
chr2_4	exon	10299511	10299590	10299511	ID=Msa0314540-mRNA-1:exon:3979;Parent=Msa0314540-mRNA-1
chr2_4	exon	10300344	10300406	10300344	ID=Msa0314540-mRNA-1:exon:3980;Parent=Msa0314540-mRNA-1
chr2_4	exon	10300527	10300633	10300527	ID=Msa0314540-mRNA-1:exon:3981;Parent=Msa0314540-mRNA-1
chr2_4	exon	10300802	10300887	10300802	ID=Msa0314540-mRNA-1:exon:3982;Parent=Msa0314540-mRNA-1
chr2_4	exon	10301350	10301391	10301350	ID=Msa0314540-mRNA-1:exon:3983;Parent=Msa0314540-mRNA-1
chr2_4	exon	10301713	10301834	10301713	ID=Msa0314540-mRNA-1:exon:3984;Parent=Msa0314540-mRNA-1
chr2_4	exon	10302705	10302816	10302705	ID=Msa0314540-mRNA-1:exon:3985;Parent=Msa0314540-mRNA-1
chr2_4	exon	10303089	10303527	10303089	ID=Msa0314540-mRNA-1:exon:3986;Parent=Msa0314540-mRNA-1
chr2_4	five_prime_UTR	10296839	10297035	10296839	ID=Msa0314540-mRNA-1:five_prime_utr;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10297036	10297353	10297036	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10298349	10298423	10298349	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10298527	10298622	10298527	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10299110	10299195	10299110	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10299281	10299401	10299281	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10299511	10299590	10299511	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10300344	10300406	10300344	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10300527	10300633	10300527	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10300802	10300887	10300802	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10301350	10301391	10301350	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10301713	10301834	10301713	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10302705	10302816	10302705	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	CDS	10303089	10303196	10303089	ID=Msa0314540-mRNA-1:cds;Parent=Msa0314540-mRNA-1
chr2_4	three_prime_UTR	10303197	10303527	10303197	ID=Msa0314540-mRNA-1:three_prime_utr;Parent=Msa0314540-mRNA-1

Gene Sequence

>Msa0314540

ATGGAATCAAAGAATCCAACAAGGTTCAAGCTAGGAAAACAATCATCATTAGCACCAGAAAGACACCGTGAAGAAGATGAAGTTCATCATGATGGTGCTGCTACTATTGATCCTGGTGTTAGGTTGATGTATTCAGCGAATGAAGGTGATGTTGATGGAATTCGTGAGGTCCTTGAATCAGGTGTTAGTGTTAATTTTAGAGATGTTGATGGAAGGACTGCTCTTCATATTGCTGCTTGTCAAGGGTTGACTCATGTTGTTCAGTTGTTGCTTGAGAAAGGAGCTGATGTTGATCCTAAAGATCAATGGGGGAGCACGCCCCTTGCAGATGCTATATTTTATAAAAACAACGATGTGATCAAACTATTGGAGAATCATGGAGCAAAGCCTCTGATGGCTTCTATGCATGTTAATCATGCCCGTGAAGTCCCCGAATATGAAATCAATCCTAAAGAACTTGATTTCACCAACAGTGTGGAGATAACAAAGGGGACTTTCTGTCTTGCGTTGTGGCGTGGGACTGAGGTTGCAGTAAAAAAGCTCGGGGAGGATGTGAGCAGTGATGAGGAGAAAGTGAAGGCTTTCAGAGACGAGCTGGCTCTGTTTCAGAAGATTCGGCATCCAAATGTAGTGCAGTTTCTTGGTGCTGTGACACAGAGTACTCCAATGATGATTGTGACAGAATATCTTCCCAAGGGAGATCTTCGTGATTTCATGAAAAGAAAAGGAGCTTTGAAACCAAGTACTGCTGTGAGATTTGCACTTGATATTGCTAGGGGTGTGGGTTATTTGCATGAAAATAAACCATCACCTATTATTCATCGTGATCTTGAGCCTTCTAATATATTGCGGGATGATTCCGGGCACCTAAAAGTTGCAGACTTTGGGGTCAGCAAGTTGCTAGCAAATAAAGAAGACAAGCCTCTAACTTGCCAAGAAACTTCTTGCCGCTATGTGGCTCCTGAGGTTTTTAAACAAGAAGAATATGACACTAAAGTGGATGTGTTCTCATTTGCACTAATTCTACAAGAGACTCTTCTTTCTTGGGTCCTACTGGTCCAGGAGGAAGAGAAGATGATTGAAGGCTGCCCTCCTTTTTCTGCAAAGCGCGATGATGAGGTTCCTAAGGTATATGCTTCAAAAGAGCGGCCACCCTTCCGAGCTCCAATCAAGCGTTATTCTCATGGAATTCGAGAGTTGATTGAAGAGTGCTGGAATGAGAATCCGGCAAAGAGGCCAACATTCAGGCAAATAATAACAAGACTGGAGTCCATCTACAATACAATTGGTCAAAAAAGGCGTTGGAAGGTTAGACCATTGAGGTGCTTTCAGAATCTTGAAGCTTTGTTGAAGAGAGATCGTTCAAATCTAAGCAGTCGGGGCAGTTCATCTCGCTCAACTTCCAGCCGTACATGA

Protein sequence

>Msa0314540

MESKNPTRFKLGKQSSLAPERHREEDEVHHDGAATIDPGVRLMYSANEGDVDGIREVLESGVSVNFRDVDGRTALHIAACQGLTHVVQLLLEKGADVDPKDQWGSTPLADAIFYKNNDVIKLLENHGAKPLMASMHVNHAREVPEYEINPKELDFTNSVEITKGTFCLALWRGTEVAVKKLGEDVSSDEEKVKAFRDELALFQKIRHPNVVQFLGAVTQSTPMMIVTEYLPKGDLRDFMKRKGALKPSTAVRFALDIARGVGYLHENKPSPIIHRDLEPSNILRDDSGHLKVADFGVSKLLANKEDKPLTCQETSCRYVAPEVFKQEEYDTKVDVFSFALILQETLLSWVLLVQEEEKMIEGCPPFSAKRDDEVPKVYASKERPPFRAPIKRYSHGIRELIEECWNENPAKRPTFRQIITRLESIYNTIGQKRRWKVRPLRCFQNLEALLKRDRSNLSSRGSSSRSTSSRT*