AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0318350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAAGCATCATATTTAAT+TGG 0.234173 2_4:+16877867 None:intergenic
CAAAGGGCCACCAGCCAAAA+TGG 0.260033 2_4:+16877690 None:intergenic
GAAATTCACCTGTGTATTAT+AGG 0.288345 2_4:+16878416 None:intergenic
CATCCGTACCATGTATAATA+TGG 0.332781 2_4:+16878457 None:intergenic
CTTTCATCCTCCTCAATGTT+GGG 0.343582 2_4:+16877897 None:intergenic
TATATTCCTCGCATCATGAT+TGG 0.373746 2_4:-16877604 Msa0318350:three_prime_UTR
GTTAATGGAGCAAGAGTTGC+GGG 0.381401 2_4:-16878051 Msa0318350:CDS
ATAAATTAGGCAAAAGATCT+TGG 0.388795 2_4:-16878516 Msa0318350:intron
TGACCGGGTGTTGAAGAATA+TGG 0.396892 2_4:-16877939 Msa0318350:three_prime_UTR
TTAGGTGTGCCGCATATATA+CGG 0.414944 2_4:-16877565 Msa0318350:three_prime_UTR
ATTTCCAATAACCAAATAAA+CGG 0.428344 2_4:+16877475 None:intergenic
CTCGCGGAAGAAAGTACAAA+AGG 0.434639 2_4:+16877540 None:intergenic
AAGCGTTGATCGACGGTTTG+AGG 0.444795 2_4:-16877985 Msa0318350:CDS
ATATTCCTCGCATCATGATT+GGG 0.449764 2_4:-16877603 Msa0318350:three_prime_UTR
CGCAAGCACATCAAGGTGAC+CGG 0.462789 2_4:-16877955 Msa0318350:exon
ACTTTCATCCTCCTCAATGT+TGG 0.469316 2_4:+16877896 None:intergenic
ATGCAGGGATATGCTATTGT+CGG 0.496724 2_4:-16877655 Msa0318350:three_prime_UTR
TCGATGAAGCTCGTCTACAT+AGG 0.503727 2_4:+16878485 None:intergenic
GCATCAAAATTGAACACAAA+GGG 0.507153 2_4:+16877674 None:intergenic
TTCAAGAAAGCGTTGATCGA+CGG 0.516090 2_4:-16877992 Msa0318350:CDS
GGCTGTTGTTGATGCTGCAG+TGG 0.521659 2_4:-16877726 Msa0318350:intron
CGATGAAGCTCGTCTACATA+GGG 0.534160 2_4:+16878486 None:intergenic
GTTGCGGGACAAGATGGAAC+GGG 0.537406 2_4:-16878036 Msa0318350:CDS
GCAAGAGTTGCGGGACAAGA+TGG 0.542408 2_4:-16878042 Msa0318350:CDS
AAACCATATTATACATGGTA+CGG 0.547991 2_4:-16878460 Msa0318350:CDS
CGAGAAAACCATATTATACA+TGG 0.548653 2_4:-16878465 Msa0318350:CDS
AGAATATGGTTCAGATGAGT+CGG 0.556570 2_4:-16877925 Msa0318350:three_prime_UTR
TGCATCAAAATTGAACACAA+AGG 0.580420 2_4:+16877673 None:intergenic
AATTGATCGCAAGCACATCA+AGG 0.602229 2_4:-16877962 Msa0318350:CDS
AAATATATTCATCGACGAGA+GGG 0.603737 2_4:+16877499 None:intergenic
TCGCGGAAGAAAGTACAAAA+GGG 0.604568 2_4:+16877541 None:intergenic
AGTTAATGGAGCAAGAGTTG+CGG 0.613051 2_4:-16878052 Msa0318350:CDS
AGTTGCGGGACAAGATGGAA+CGG 0.623306 2_4:-16878037 Msa0318350:CDS
GTCGGACAATCCCAACATTG+AGG 0.630524 2_4:-16877907 Msa0318350:three_prime_UTR
GCAAGCACATCAAGGTGACC+GGG 0.633386 2_4:-16877954 Msa0318350:exon
AAAATATATTCATCGACGAG+AGG 0.634794 2_4:+16877498 None:intergenic
TAAGACACACGCTCAACTCG+CGG 0.650883 2_4:+16877524 None:intergenic
GAACCATATTCTTCAACACC+CGG 0.652425 2_4:+16877936 None:intergenic
CAAGACCCAATCATGATGCG+AGG 0.663868 2_4:+16877598 None:intergenic
TGATCGCTCCTATAATACAC+AGG 0.675215 2_4:-16878424 Msa0318350:intron
GGACAATCCCAACATTGAGG+AGG 0.682139 2_4:-16877904 Msa0318350:three_prime_UTR
CAAAAGGGACCGTATATATG+CGG 0.692290 2_4:+16877556 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTAACATAATTTTAG+GGG - chr2_4:16878604-16878623 Msa0318350:intron 10.0%
!!! TTAACTTTTTGTGATAAATT+AGG - chr2_4:16877503-16877522 Msa0318350:three_prime_UTR 15.0%
!!! TTAACTTTTTGTGATAAATT+AGG - chr2_4:16877503-16877522 Msa0318350:three_prime_UTR 15.0%
!! ACGTATATATTTAATTAGTC+AGG - chr2_4:16877468-16877487 Msa0318350:three_prime_UTR 20.0%
!!! GAATATATTTTCCGTTTATT+TGG - chr2_4:16878546-16878565 Msa0318350:intron 20.0%
!! ATTTCCAATAACCAAATAAA+CGG + chr2_4:16878560-16878579 None:intergenic 20.0%
!! ACGTATATATTTAATTAGTC+AGG - chr2_4:16877468-16877487 Msa0318350:three_prime_UTR 20.0%
!!! GAATATATTTTCCGTTTATT+TGG - chr2_4:16878546-16878565 Msa0318350:intron 20.0%
!! ATTTCCAATAACCAAATAAA+CGG + chr2_4:16878560-16878579 None:intergenic 20.0%
! ATAAATTAGGCAAAAGATCT+TGG - chr2_4:16877516-16877535 Msa0318350:three_prime_UTR 25.0%
! AAACCATATTATACATGGTA+CGG - chr2_4:16877572-16877591 Msa0318350:three_prime_UTR 25.0%
! AATAGTAACATGAATATTCC+TGG + chr2_4:16877698-16877717 None:intergenic 25.0%
!! TCTGAAGCATCATATTTAAT+TGG + chr2_4:16878168-16878187 None:intergenic 25.0%
!!! ATGATGCTTTAGATAGTTTT+AGG - chr2_4:16878220-16878239 Msa0318350:intron 25.0%
!! TTTTCCGTTTATTTGGTTAT+TGG - chr2_4:16878553-16878572 Msa0318350:intron 25.0%
! ATAAATTAGGCAAAAGATCT+TGG - chr2_4:16877516-16877535 Msa0318350:three_prime_UTR 25.0%
! AAACCATATTATACATGGTA+CGG - chr2_4:16877572-16877591 Msa0318350:three_prime_UTR 25.0%
! AATAGTAACATGAATATTCC+TGG + chr2_4:16877698-16877717 None:intergenic 25.0%
!! TCTGAAGCATCATATTTAAT+TGG + chr2_4:16878168-16878187 None:intergenic 25.0%
!!! ATGATGCTTTAGATAGTTTT+AGG - chr2_4:16878220-16878239 Msa0318350:intron 25.0%
!! TTTTCCGTTTATTTGGTTAT+TGG - chr2_4:16878553-16878572 Msa0318350:intron 25.0%
CGAGAAAACCATATTATACA+TGG - chr2_4:16877567-16877586 Msa0318350:three_prime_UTR 30.0%
GAAATTCACCTGTGTATTAT+AGG + chr2_4:16877619-16877638 None:intergenic 30.0%
CCAGGTTATTATTATGGTTT+CGG + chr2_4:16877751-16877770 None:intergenic 30.0%
TAAATTAGCCAAAGACGAAT+AGG - chr2_4:16877854-16877873 Msa0318350:three_prime_UTR 30.0%
CAAAATAACCTATTCGTCTT+TGG + chr2_4:16877865-16877884 None:intergenic 30.0%
!! AAGACGAATAGGTTATTTTG+TGG - chr2_4:16877865-16877884 Msa0318350:three_prime_UTR 30.0%
!! AGACGAATAGGTTATTTTGT+GGG - chr2_4:16877866-16877885 Msa0318350:three_prime_UTR 30.0%
! TTTTGTGCATGTGAAGTTAA+TGG - chr2_4:16877966-16877985 Msa0318350:CDS 30.0%
!!! ATAGTTTTAGGCTAGTTTTG+TGG - chr2_4:16878232-16878251 Msa0318350:intron 30.0%
!!! GCATTTTTTAGGTTCCATTT+TGG - chr2_4:16878328-16878347 Msa0318350:intron 30.0%
GCATCAAAATTGAACACAAA+GGG + chr2_4:16878361-16878380 None:intergenic 30.0%
TGCATCAAAATTGAACACAA+AGG + chr2_4:16878362-16878381 None:intergenic 30.0%
!! TTTGTGTTCAATTTTGATGC+AGG - chr2_4:16878361-16878380 Msa0318350:intron 30.0%
!! TTGTGTTCAATTTTGATGCA+GGG - chr2_4:16878362-16878381 Msa0318350:intron 30.0%
AAATATATTCATCGACGAGA+GGG + chr2_4:16878536-16878555 None:intergenic 30.0%
AAAATATATTCATCGACGAG+AGG + chr2_4:16878537-16878556 None:intergenic 30.0%
CGAGAAAACCATATTATACA+TGG - chr2_4:16877567-16877586 Msa0318350:three_prime_UTR 30.0%
GAAATTCACCTGTGTATTAT+AGG + chr2_4:16877619-16877638 None:intergenic 30.0%
CCAGGTTATTATTATGGTTT+CGG + chr2_4:16877751-16877770 None:intergenic 30.0%
TAAATTAGCCAAAGACGAAT+AGG - chr2_4:16877854-16877873 Msa0318350:three_prime_UTR 30.0%
CAAAATAACCTATTCGTCTT+TGG + chr2_4:16877865-16877884 None:intergenic 30.0%
!! AAGACGAATAGGTTATTTTG+TGG - chr2_4:16877865-16877884 Msa0318350:three_prime_UTR 30.0%
!! AGACGAATAGGTTATTTTGT+GGG - chr2_4:16877866-16877885 Msa0318350:three_prime_UTR 30.0%
! TTTTGTGCATGTGAAGTTAA+TGG - chr2_4:16877966-16877985 Msa0318350:CDS 30.0%
!!! ATAGTTTTAGGCTAGTTTTG+TGG - chr2_4:16878232-16878251 Msa0318350:intron 30.0%
!!! GCATTTTTTAGGTTCCATTT+TGG - chr2_4:16878328-16878347 Msa0318350:intron 30.0%
GCATCAAAATTGAACACAAA+GGG + chr2_4:16878361-16878380 None:intergenic 30.0%
TGCATCAAAATTGAACACAA+AGG + chr2_4:16878362-16878381 None:intergenic 30.0%
!! TTTGTGTTCAATTTTGATGC+AGG - chr2_4:16878361-16878380 Msa0318350:intron 30.0%
!! TTGTGTTCAATTTTGATGCA+GGG - chr2_4:16878362-16878381 Msa0318350:intron 30.0%
AAATATATTCATCGACGAGA+GGG + chr2_4:16878536-16878555 None:intergenic 30.0%
AAAATATATTCATCGACGAG+AGG + chr2_4:16878537-16878556 None:intergenic 30.0%
CATCCGTACCATGTATAATA+TGG + chr2_4:16877578-16877597 None:intergenic 35.0%
AACTTTCATTACATTCTGCC+AGG - chr2_4:16877677-16877696 Msa0318350:three_prime_UTR 35.0%
CCGAAACCATAATAATAACC+TGG - chr2_4:16877748-16877767 Msa0318350:intron 35.0%
!! GACGAATAGGTTATTTTGTG+GGG - chr2_4:16877867-16877886 Msa0318350:three_prime_UTR 35.0%
!! ATAGGTTATTTTGTGGGGAT+AGG - chr2_4:16877872-16877891 Msa0318350:three_prime_UTR 35.0%
AGAATATGGTTCAGATGAGT+CGG - chr2_4:16878107-16878126 Msa0318350:intron 35.0%
! GCATTTATGCTCTTCTGTTT+TGG - chr2_4:16878285-16878304 Msa0318350:intron 35.0%
!!! TTTTTAGGTTCCATTTTGGC+TGG - chr2_4:16878332-16878351 Msa0318350:intron 35.0%
TATATTCCTCGCATCATGAT+TGG - chr2_4:16878428-16878447 Msa0318350:CDS 35.0%
ATATTCCTCGCATCATGATT+GGG - chr2_4:16878429-16878448 Msa0318350:CDS 35.0%
!!! TCATGATTGGGTCTTGTTTT+TGG - chr2_4:16878441-16878460 Msa0318350:CDS 35.0%
!!! CATGATTGGGTCTTGTTTTT+GGG - chr2_4:16878442-16878461 Msa0318350:CDS 35.0%
!!! ATGATTGGGTCTTGTTTTTG+GGG - chr2_4:16878443-16878462 Msa0318350:CDS 35.0%
CATCCGTACCATGTATAATA+TGG + chr2_4:16877578-16877597 None:intergenic 35.0%
AACTTTCATTACATTCTGCC+AGG - chr2_4:16877677-16877696 Msa0318350:three_prime_UTR 35.0%
CCGAAACCATAATAATAACC+TGG - chr2_4:16877748-16877767 Msa0318350:intron 35.0%
!! GACGAATAGGTTATTTTGTG+GGG - chr2_4:16877867-16877886 Msa0318350:three_prime_UTR 35.0%
!! ATAGGTTATTTTGTGGGGAT+AGG - chr2_4:16877872-16877891 Msa0318350:three_prime_UTR 35.0%
AGAATATGGTTCAGATGAGT+CGG - chr2_4:16878107-16878126 Msa0318350:intron 35.0%
! GCATTTATGCTCTTCTGTTT+TGG - chr2_4:16878285-16878304 Msa0318350:intron 35.0%
!!! TTTTTAGGTTCCATTTTGGC+TGG - chr2_4:16878332-16878351 Msa0318350:intron 35.0%
TATATTCCTCGCATCATGAT+TGG - chr2_4:16878428-16878447 Msa0318350:CDS 35.0%
ATATTCCTCGCATCATGATT+GGG - chr2_4:16878429-16878448 Msa0318350:CDS 35.0%
!!! TCATGATTGGGTCTTGTTTT+TGG - chr2_4:16878441-16878460 Msa0318350:CDS 35.0%
!!! CATGATTGGGTCTTGTTTTT+GGG - chr2_4:16878442-16878461 Msa0318350:CDS 35.0%
!!! ATGATTGGGTCTTGTTTTTG+GGG - chr2_4:16878443-16878462 Msa0318350:CDS 35.0%
TGATCGCTCCTATAATACAC+AGG - chr2_4:16877608-16877627 Msa0318350:three_prime_UTR 40.0%
TTTCTACTCTGACAGTATGC+TGG - chr2_4:16877635-16877654 Msa0318350:three_prime_UTR 40.0%
TTCTACTCTGACAGTATGCT+GGG - chr2_4:16877636-16877655 Msa0318350:three_prime_UTR 40.0%
ACAGGGCCAGGTTATTATTA+TGG + chr2_4:16877757-16877776 None:intergenic 40.0%
TTGTGGGGATAGGAATATCA+TGG - chr2_4:16877882-16877901 Msa0318350:three_prime_UTR 40.0%
CACAAAATGCTGCAAGACAA+AGG + chr2_4:16877953-16877972 None:intergenic 40.0%
AGTTAATGGAGCAAGAGTTG+CGG - chr2_4:16877980-16877999 Msa0318350:CDS 40.0%
!! TTCAAGAAAGCGTTGATCGA+CGG - chr2_4:16878040-16878059 Msa0318350:CDS 40.0%
AATTGATCGCAAGCACATCA+AGG - chr2_4:16878070-16878089 Msa0318350:CDS 40.0%
GAACCATATTCTTCAACACC+CGG + chr2_4:16878099-16878118 None:intergenic 40.0%
CTTTCATCCTCCTCAATGTT+GGG + chr2_4:16878138-16878157 None:intergenic 40.0%
ACTTTCATCCTCCTCAATGT+TGG + chr2_4:16878139-16878158 None:intergenic 40.0%
!!! ATGCTGCAGTGGCATTTTTT+AGG - chr2_4:16878317-16878336 Msa0318350:intron 40.0%
ATGCAGGGATATGCTATTGT+CGG - chr2_4:16878377-16878396 Msa0318350:intron 40.0%
!!! ATTGTCGGCATCTTTTTTGC+TGG - chr2_4:16878392-16878411 Msa0318350:intron 40.0%
TTAGGTGTGCCGCATATATA+CGG - chr2_4:16878467-16878486 Msa0318350:CDS 40.0%
CAAAAGGGACCGTATATATG+CGG + chr2_4:16878479-16878498 None:intergenic 40.0%
! TCGCGGAAGAAAGTACAAAA+GGG + chr2_4:16878494-16878513 None:intergenic 40.0%
TGATCGCTCCTATAATACAC+AGG - chr2_4:16877608-16877627 Msa0318350:three_prime_UTR 40.0%
TTTCTACTCTGACAGTATGC+TGG - chr2_4:16877635-16877654 Msa0318350:three_prime_UTR 40.0%
TTCTACTCTGACAGTATGCT+GGG - chr2_4:16877636-16877655 Msa0318350:three_prime_UTR 40.0%
ACAGGGCCAGGTTATTATTA+TGG + chr2_4:16877757-16877776 None:intergenic 40.0%
TTGTGGGGATAGGAATATCA+TGG - chr2_4:16877882-16877901 Msa0318350:three_prime_UTR 40.0%
CACAAAATGCTGCAAGACAA+AGG + chr2_4:16877953-16877972 None:intergenic 40.0%
AGTTAATGGAGCAAGAGTTG+CGG - chr2_4:16877980-16877999 Msa0318350:CDS 40.0%
!! TTCAAGAAAGCGTTGATCGA+CGG - chr2_4:16878040-16878059 Msa0318350:CDS 40.0%
AATTGATCGCAAGCACATCA+AGG - chr2_4:16878070-16878089 Msa0318350:CDS 40.0%
GAACCATATTCTTCAACACC+CGG + chr2_4:16878099-16878118 None:intergenic 40.0%
CTTTCATCCTCCTCAATGTT+GGG + chr2_4:16878138-16878157 None:intergenic 40.0%
ACTTTCATCCTCCTCAATGT+TGG + chr2_4:16878139-16878158 None:intergenic 40.0%
!!! ATGCTGCAGTGGCATTTTTT+AGG - chr2_4:16878317-16878336 Msa0318350:intron 40.0%
ATGCAGGGATATGCTATTGT+CGG - chr2_4:16878377-16878396 Msa0318350:intron 40.0%
!!! ATTGTCGGCATCTTTTTTGC+TGG - chr2_4:16878392-16878411 Msa0318350:intron 40.0%
TTAGGTGTGCCGCATATATA+CGG - chr2_4:16878467-16878486 Msa0318350:CDS 40.0%
CAAAAGGGACCGTATATATG+CGG + chr2_4:16878479-16878498 None:intergenic 40.0%
! TCGCGGAAGAAAGTACAAAA+GGG + chr2_4:16878494-16878513 None:intergenic 40.0%
CGATGAAGCTCGTCTACATA+GGG + chr2_4:16877549-16877568 None:intergenic 45.0%
TCGATGAAGCTCGTCTACAT+AGG + chr2_4:16877550-16877569 None:intergenic 45.0%
TAAGCCACCAAGCTTGAACA+GGG + chr2_4:16877774-16877793 None:intergenic 45.0%
GTTAATGGAGCAAGAGTTGC+GGG - chr2_4:16877981-16878000 Msa0318350:CDS 45.0%
!! TGACCGGGTGTTGAAGAATA+TGG - chr2_4:16878093-16878112 Msa0318350:intron 45.0%
!!! TTAGGTTCCATTTTGGCTGG+TGG - chr2_4:16878335-16878354 Msa0318350:intron 45.0%
!!! GGCATCTTTTTTGCTGGTTG+TGG - chr2_4:16878398-16878417 Msa0318350:intron 45.0%
!!! GGGTCTTGTTTTTGGGGTTT+AGG - chr2_4:16878449-16878468 Msa0318350:CDS 45.0%
! CTCGCGGAAGAAAGTACAAA+AGG + chr2_4:16878495-16878514 None:intergenic 45.0%
CGATGAAGCTCGTCTACATA+GGG + chr2_4:16877549-16877568 None:intergenic 45.0%
TCGATGAAGCTCGTCTACAT+AGG + chr2_4:16877550-16877569 None:intergenic 45.0%
TAAGCCACCAAGCTTGAACA+GGG + chr2_4:16877774-16877793 None:intergenic 45.0%
GTTAATGGAGCAAGAGTTGC+GGG - chr2_4:16877981-16878000 Msa0318350:CDS 45.0%
!! TGACCGGGTGTTGAAGAATA+TGG - chr2_4:16878093-16878112 Msa0318350:intron 45.0%
!!! TTAGGTTCCATTTTGGCTGG+TGG - chr2_4:16878335-16878354 Msa0318350:intron 45.0%
!!! GGCATCTTTTTTGCTGGTTG+TGG - chr2_4:16878398-16878417 Msa0318350:intron 45.0%
!!! GGGTCTTGTTTTTGGGGTTT+AGG - chr2_4:16878449-16878468 Msa0318350:CDS 45.0%
! CTCGCGGAAGAAAGTACAAA+AGG + chr2_4:16878495-16878514 None:intergenic 45.0%
!!! TATTTAATTAACATAATTTT+AGG - chr2_4:16878602-16878621 Msa0318350:intron 5.0%
!!! ATTTAATTAACATAATTTTA+GGG - chr2_4:16878603-16878622 Msa0318350:intron 5.0%
!!! TATTTAATTAACATAATTTT+AGG - chr2_4:16878602-16878621 Msa0318350:intron 5.0%
!!! ATTTAATTAACATAATTTTA+GGG - chr2_4:16878603-16878622 Msa0318350:intron 5.0%
CTAAGCCACCAAGCTTGAAC+AGG + chr2_4:16877775-16877794 None:intergenic 50.0%
AGTTGCGGGACAAGATGGAA+CGG - chr2_4:16877995-16878014 Msa0318350:CDS 50.0%
!! AAGCGTTGATCGACGGTTTG+AGG - chr2_4:16878047-16878066 Msa0318350:CDS 50.0%
GTCGGACAATCCCAACATTG+AGG - chr2_4:16878125-16878144 Msa0318350:intron 50.0%
GGACAATCCCAACATTGAGG+AGG - chr2_4:16878128-16878147 Msa0318350:intron 50.0%
CAAGACCCAATCATGATGCG+AGG + chr2_4:16878437-16878456 None:intergenic 50.0%
TAAGACACACGCTCAACTCG+CGG + chr2_4:16878511-16878530 None:intergenic 50.0%
CTAAGCCACCAAGCTTGAAC+AGG + chr2_4:16877775-16877794 None:intergenic 50.0%
AGTTGCGGGACAAGATGGAA+CGG - chr2_4:16877995-16878014 Msa0318350:CDS 50.0%
!! AAGCGTTGATCGACGGTTTG+AGG - chr2_4:16878047-16878066 Msa0318350:CDS 50.0%
GTCGGACAATCCCAACATTG+AGG - chr2_4:16878125-16878144 Msa0318350:intron 50.0%
GGACAATCCCAACATTGAGG+AGG - chr2_4:16878128-16878147 Msa0318350:intron 50.0%
CAAGACCCAATCATGATGCG+AGG + chr2_4:16878437-16878456 None:intergenic 50.0%
TAAGACACACGCTCAACTCG+CGG + chr2_4:16878511-16878530 None:intergenic 50.0%
AACCTGGCCCTGTTCAAGCT+TGG - chr2_4:16877764-16877783 Msa0318350:intron 55.0%
GCAAGAGTTGCGGGACAAGA+TGG - chr2_4:16877990-16878009 Msa0318350:CDS 55.0%
GTTGCGGGACAAGATGGAAC+GGG - chr2_4:16877996-16878015 Msa0318350:CDS 55.0%
CGCAAGCACATCAAGGTGAC+CGG - chr2_4:16878077-16878096 Msa0318350:intron 55.0%
GCAAGCACATCAAGGTGACC+GGG - chr2_4:16878078-16878097 Msa0318350:intron 55.0%
GGCTGTTGTTGATGCTGCAG+TGG - chr2_4:16878306-16878325 Msa0318350:intron 55.0%
CAAAGGGCCACCAGCCAAAA+TGG + chr2_4:16878345-16878364 None:intergenic 55.0%
AACCTGGCCCTGTTCAAGCT+TGG - chr2_4:16877764-16877783 Msa0318350:intron 55.0%
GCAAGAGTTGCGGGACAAGA+TGG - chr2_4:16877990-16878009 Msa0318350:CDS 55.0%
GTTGCGGGACAAGATGGAAC+GGG - chr2_4:16877996-16878015 Msa0318350:CDS 55.0%
CGCAAGCACATCAAGGTGAC+CGG - chr2_4:16878077-16878096 Msa0318350:intron 55.0%
GCAAGCACATCAAGGTGACC+GGG - chr2_4:16878078-16878097 Msa0318350:intron 55.0%
GGCTGTTGTTGATGCTGCAG+TGG - chr2_4:16878306-16878325 Msa0318350:intron 55.0%
CAAAGGGCCACCAGCCAAAA+TGG + chr2_4:16878345-16878364 None:intergenic 55.0%
CACCAAGCTTGAACAGGGCC+AGG + chr2_4:16877769-16877788 None:intergenic 60.0%
CTGGCCCTGTTCAAGCTTGG+TGG - chr2_4:16877767-16877786 Msa0318350:intron 60.0%
CACCAAGCTTGAACAGGGCC+AGG + chr2_4:16877769-16877788 None:intergenic 60.0%
CTGGCCCTGTTCAAGCTTGG+TGG - chr2_4:16877767-16877786 Msa0318350:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 16877428 16878626 16877428 ID=Msa0318350;Name=Msa0318350
chr2_4 mRNA 16877428 16878626 16877428 ID=Msa0318350-mRNA-1;Parent=Msa0318350;Name=Msa0318350-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0.66|0.75|0.75|0.66|0.5|4|480|79
chr2_4 exon 16877428 16877743 16877428 ID=Msa0318350-mRNA-1:exon:6387;Parent=Msa0318350-mRNA-1
chr2_4 exon 16877795 16878090 16877795 ID=Msa0318350-mRNA-1:exon:6386;Parent=Msa0318350-mRNA-1
chr2_4 exon 16878425 16878529 16878425 ID=Msa0318350-mRNA-1:exon:6385;Parent=Msa0318350-mRNA-1
chr2_4 exon 16878624 16878626 16878624 ID=Msa0318350-mRNA-1:exon:6384;Parent=Msa0318350-mRNA-1
chr2_4 CDS 16878624 16878626 16878624 ID=Msa0318350-mRNA-1:cds;Parent=Msa0318350-mRNA-1
chr2_4 CDS 16878425 16878529 16878425 ID=Msa0318350-mRNA-1:cds;Parent=Msa0318350-mRNA-1
chr2_4 CDS 16877959 16878090 16877959 ID=Msa0318350-mRNA-1:cds;Parent=Msa0318350-mRNA-1
chr2_4 three_prime_UTR 16877795 16877958 16877795 ID=Msa0318350-mRNA-1:three_prime_utr;Parent=Msa0318350-mRNA-1
chr2_4 three_prime_UTR 16877428 16877743 16877428 ID=Msa0318350-mRNA-1:three_prime_utr;Parent=Msa0318350-mRNA-1
Gene Sequence

>Msa0318350

ATGGCAAAAGATCTTGGAAGAGATCCCTATGTAGACGAGCTTCATCGAGAAAACCATATTATACATGGTACGGATGTCTACGTTGATGATCGCTCCTATAATACACAGCATTTTGTGCATGTGAAGTTAATGGAGCAAGAGTTGCGGGACAAGATGGAACGGGACGCTGAGCAATATAGAGCATTTCAAGAAAGCGTTGATCGACGGTTTGAGGAATTGATCGCAAGCACATCAAGGTGA
Protein sequence

>Msa0318350

MAKDLGRDPYVDELHRENHIIHGTDVYVDDRSYNTQHFVHVKLMEQELRDKMERDAEQYRAFQESVDRRFEELIASTSR*