AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0735310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735310 MtrunA17_Chr5g0432431 94.074 135 8 0 1 135 1 135 3.29e-91 260
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735310 AT2G30330.1 59.649 114 46 0 22 135 37 150 1.18e-46 148
Msa0735310 AT2G30330.2 48.039 102 52 1 22 123 37 137 1.06e-28 102

Find 25 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGCCTTGTATCTTTATTC+TGG 0.213211 5_1:-50461705 Msa0735310:three_prime_UTR
TTTGATGATGAATGTGAATT+AGG 0.250869 5_1:+50463929 None:intergenic
TATTTATTAGTGATTAGTTT+TGG 0.348003 5_1:-50461554 Msa0735310:three_prime_UTR
TGTAGCACTGGTTGGTGATT+TGG 0.356599 5_1:-50461635 Msa0735310:three_prime_UTR
TACGTATATTTGACAAATCA+TGG 0.369629 5_1:-50461610 Msa0735310:three_prime_UTR
TTGAACTTAGATACTCTGTT+TGG 0.389138 5_1:-50464035 Msa0735310:five_prime_UTR
AAATTGGAGACTTTGAGAAT+TGG 0.397575 5_1:-50461947 Msa0735310:CDS
TTCACCAAGAGTGATCTTGC+AGG 0.449401 5_1:-50461872 Msa0735310:exon
ACGCGTCTCGGATCTGTTAG+TGG 0.461253 5_1:-50463845 Msa0735310:CDS
TGAGAATTGGATGAAGATCA+TGG 0.464193 5_1:-50461934 Msa0735310:CDS
GGCTTCTAATGATGTAGCAC+TGG 0.472814 5_1:-50461647 Msa0735310:three_prime_UTR
TTCGAGATTTGTGAAACAAA+CGG 0.473259 5_1:-50463740 Msa0735310:CDS
TTGTTTCTCAACAAAATAAG+TGG 0.494592 5_1:-50461740 Msa0735310:three_prime_UTR
TTCTCTTAACACTGCTCTCA+AGG 0.509830 5_1:-50463695 Msa0735310:intron
ACGCGCAGAATCAAAGGGAT+TGG 0.518596 5_1:-50463968 Msa0735310:CDS
AAGAAAAGCAGAACGCGTCT+CGG 0.526602 5_1:-50463857 Msa0735310:CDS
TAACACACGCGCAGAATCAA+AGG 0.527349 5_1:-50463974 Msa0735310:CDS
AATACAAAGAAACAAGTGAA+TGG 0.531661 5_1:-50464010 Msa0735310:exon
GCAACCTGCAAGATCACTCT+TGG 0.532927 5_1:+50461868 None:intergenic
CTGTTAGTGGAAGCAGTGAA+TGG 0.540308 5_1:-50463832 Msa0735310:CDS
TCTAATGATGTAGCACTGGT+TGG 0.573738 5_1:-50461643 Msa0735310:three_prime_UTR
AACACACGCGCAGAATCAAA+GGG 0.580106 5_1:-50463973 Msa0735310:CDS
TTGTGAAACAAACGGATCAG+TGG 0.597090 5_1:-50463732 Msa0735310:CDS
ACATCCAGAATAAAGATACA+AGG 0.617763 5_1:+50461701 None:intergenic
TTAGTGGAAGCAGTGAATGG+CGG 0.745571 5_1:-50463829 Msa0735310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTAGTTAATAATGATA+AGG - chr5_1:50462126-50462145 Msa0735310:intron 10.0%
!!! TGTAAAATTTTATTAGATTA+AGG - chr5_1:50463825-50463844 Msa0735310:CDS 10.0%
!! AATAGAAAAATTAATGGAAA+GGG + chr5_1:50463783-50463802 None:intergenic 15.0%
!!! ATAAAATTTTACATGATGTT+GGG + chr5_1:50463818-50463837 None:intergenic 15.0%
!!! AATAAAATTTTACATGATGT+TGG + chr5_1:50463819-50463838 None:intergenic 15.0%
!!! TTTTAAATGATGAATATGAT+TGG - chr5_1:50463921-50463940 Msa0735310:CDS 15.0%
!!! TATTTATTAGTGATTAGTTT+TGG - chr5_1:50464035-50464054 Msa0735310:five_prime_UTR 15.0%
!!! AAATGTCACAAAAACAATTT+TGG - chr5_1:50462604-50462623 Msa0735310:intron 20.0%
!! AATATGATTCCACAATATAA+AGG + chr5_1:50462828-50462847 None:intergenic 20.0%
!!! CATCATAACCAAAATTATTT+TGG + chr5_1:50462994-50463013 None:intergenic 20.0%
!!! CAAAATAATTTTGGTTATGA+TGG - chr5_1:50462992-50463011 Msa0735310:intron 20.0%
!! TTTGTAAATGTTTATTCTTG+TGG - chr5_1:50463050-50463069 Msa0735310:intron 20.0%
!!! AAACTTTTTTTTTAACTCGT+AGG - chr5_1:50463619-50463638 Msa0735310:intron 20.0%
!! GAATAGAAAAATTAATGGAA+AGG + chr5_1:50463784-50463803 None:intergenic 20.0%
!!! TCCATTAATTTTTCTATTCT+AGG - chr5_1:50463785-50463804 Msa0735310:CDS 20.0%
! AATACAAAGAAACAAGTGAA+TGG - chr5_1:50461579-50461598 Msa0735310:three_prime_UTR 25.0%
!!! TTTGATGATGAATGTGAATT+AGG + chr5_1:50461663-50461682 None:intergenic 25.0%
!! ATTGATAAGTTAGCTTATAG+CGG - chr5_1:50462009-50462028 Msa0735310:intron 25.0%
! GAAGTTAGATACTAAATTGA+CGG - chr5_1:50462389-50462408 Msa0735310:intron 25.0%
!! TAGTTTATGGACTAAATTGA+TGG - chr5_1:50462451-50462470 Msa0735310:intron 25.0%
!!! TGGTAGTGGATTATTAAATT+GGG + chr5_1:50462677-50462696 None:intergenic 25.0%
!!! TTGGTAGTGGATTATTAAAT+TGG + chr5_1:50462678-50462697 None:intergenic 25.0%
! TTTAATAATCCACTACCAAT+TGG - chr5_1:50462679-50462698 Msa0735310:intron 25.0%
!! TTCCTTCTCCAAAATAATTT+TGG - chr5_1:50462983-50463002 Msa0735310:intron 25.0%
!!! AACCAAAATTATTTTGGAGA+AGG + chr5_1:50462988-50463007 None:intergenic 25.0%
! TCTTCTCTTCAAATATTCAT+TGG - chr5_1:50463156-50463175 Msa0735310:intron 25.0%
! CTTCTCTTCAAATATTCATT+GGG - chr5_1:50463157-50463176 Msa0735310:intron 25.0%
! TTAAGGTTGTTAAAGATTCA+AGG - chr5_1:50463279-50463298 Msa0735310:intron 25.0%
! TTTGAAAGAACACTGATAAA+TGG - chr5_1:50463384-50463403 Msa0735310:intron 25.0%
! ATATGAGCAAGAATAAAGAT+GGG - chr5_1:50463454-50463473 Msa0735310:intron 25.0%
!!! TTTTTTAACTCGTAGGAAAT+TGG - chr5_1:50463626-50463645 Msa0735310:intron 25.0%
! GCCTAGAATAGAAAAATTAA+TGG + chr5_1:50463789-50463808 None:intergenic 25.0%
!! TTGTTTCTCAACAAAATAAG+TGG - chr5_1:50463849-50463868 Msa0735310:CDS 25.0%
! TACGTATATTTGACAAATCA+TGG - chr5_1:50463979-50463998 Msa0735310:CDS 25.0%
TTGAACTTAGATACTCTGTT+TGG - chr5_1:50461554-50461573 Msa0735310:three_prime_UTR 30.0%
TTCGAGATTTGTGAAACAAA+CGG - chr5_1:50461849-50461868 Msa0735310:three_prime_UTR 30.0%
CTGATTGAATTTGTAGTGTT+TGG - chr5_1:50462071-50462090 Msa0735310:intron 30.0%
TGACGGAATTCATATACTTT+AGG - chr5_1:50462406-50462425 Msa0735310:intron 30.0%
GACGGAATTCATATACTTTA+GGG - chr5_1:50462407-50462426 Msa0735310:intron 30.0%
GAAGAGAGAGTAATAGTTTA+TGG - chr5_1:50462438-50462457 Msa0735310:intron 30.0%
TTCCAAAAATAAATTCCCCA+AGG - chr5_1:50462478-50462497 Msa0735310:intron 30.0%
TTCCACAATATAAAGGAAAG+TGG + chr5_1:50462821-50462840 None:intergenic 30.0%
ACATAACATACTTCCATTCA+GGG + chr5_1:50462913-50462932 None:intergenic 30.0%
ATTCACCAATAACCCAAAAA+TGG + chr5_1:50463026-50463045 None:intergenic 30.0%
!! TTGTGGTTTGTTCCAAATTA+TGG - chr5_1:50463067-50463086 Msa0735310:intron 30.0%
CTTTATTGTGCACCATAATT+TGG + chr5_1:50463082-50463101 None:intergenic 30.0%
CATTAACATGAACATGTACA+AGG + chr5_1:50463256-50463275 None:intergenic 30.0%
TGTTCATGTTAATGTGCTTA+AGG - chr5_1:50463262-50463281 Msa0735310:intron 30.0%
TGAAATATGCATCAATCTTG+TGG - chr5_1:50463323-50463342 Msa0735310:intron 30.0%
CATATGAGCAAGAATAAAGA+TGG - chr5_1:50463453-50463472 Msa0735310:intron 30.0%
TATGAGCAAGAATAAAGATG+GGG - chr5_1:50463455-50463474 Msa0735310:intron 30.0%
! AAATTGGAGACTTTGAGAAT+TGG - chr5_1:50463642-50463661 Msa0735310:intron 30.0%
ACATCCAGAATAAAGATACA+AGG + chr5_1:50463891-50463910 None:intergenic 30.0%
!!! TGCATCTTTCTTCGCTTTTT+CGG + chr5_1:50461710-50461729 None:intergenic 35.0%
!! TATAGCGGATGAACTTATAG+AGG - chr5_1:50462024-50462043 Msa0735310:intron 35.0%
AGCTCATTTGAAAGTCTGTT+TGG + chr5_1:50462281-50462300 None:intergenic 35.0%
!! CTCCTTGGGGAATTTATTTT+TGG + chr5_1:50462483-50462502 None:intergenic 35.0%
CAAAAATAAATTCCCCAAGG+AGG - chr5_1:50462481-50462500 Msa0735310:intron 35.0%
ACCAAAGGCAATCATATTTG+AGG + chr5_1:50462532-50462551 None:intergenic 35.0%
CAAATATGATTGCCTTTGGT+AGG - chr5_1:50462532-50462551 Msa0735310:intron 35.0%
CACATATATACTCCTACCAA+AGG + chr5_1:50462547-50462566 None:intergenic 35.0%
GTGAATTTCATCTAGAGAGA+TGG - chr5_1:50462564-50462583 Msa0735310:intron 35.0%
TTTCAGAATGCTCAGATTGT+TGG - chr5_1:50462634-50462653 Msa0735310:intron 35.0%
TATAGTGTTCCAATTGGTAG+TGG + chr5_1:50462691-50462710 None:intergenic 35.0%
CATCAGTATAGTGTTCCAAT+TGG + chr5_1:50462697-50462716 None:intergenic 35.0%
TGATGTTGATCTGACATAAG+AGG - chr5_1:50462712-50462731 Msa0735310:intron 35.0%
!!! ATAAGAGGCGTTTTCTTATG+TGG - chr5_1:50462727-50462746 Msa0735310:intron 35.0%
!!! TAAGAGGCGTTTTCTTATGT+GGG - chr5_1:50462728-50462747 Msa0735310:intron 35.0%
!! TCCATCTTTTTGGCATCTAT+GGG - chr5_1:50462766-50462785 Msa0735310:intron 35.0%
AAGGTTTGTGATCATGATCT+TGG + chr5_1:50462881-50462900 None:intergenic 35.0%
GACATAACATACTTCCATTC+AGG + chr5_1:50462914-50462933 None:intergenic 35.0%
TTGGAGAAGGAATCTAACTA+TGG + chr5_1:50462975-50462994 None:intergenic 35.0%
!!! ATGGTTGTCATGCCATTTTT+GGG - chr5_1:50463011-50463030 Msa0735310:intron 35.0%
!!! TCATGCCATTTTTGGGTTAT+TGG - chr5_1:50463018-50463037 Msa0735310:intron 35.0%
!! CCTTCTTTCTTGAGTTGATT+GGG - chr5_1:50463116-50463135 Msa0735310:intron 35.0%
TCAAGGATAATGCAAGAGTT+TGG - chr5_1:50463296-50463315 Msa0735310:intron 35.0%
TAATCTGTATTAGTCACGTC+AGG - chr5_1:50463531-50463550 Msa0735310:intron 35.0%
TGAGAATTGGATGAAGATCA+TGG - chr5_1:50463655-50463674 Msa0735310:intron 35.0%
!! GTAGCCTTGTATCTTTATTC+TGG - chr5_1:50463884-50463903 Msa0735310:CDS 35.0%
!!! CTTCGCTTTTTCGGTTTGAT+TGG + chr5_1:50461701-50461720 None:intergenic 40.0%
TTGTGAAACAAACGGATCAG+TGG - chr5_1:50461857-50461876 Msa0735310:three_prime_UTR 40.0%
TTCTCTTAACACTGCTCTCA+AGG - chr5_1:50461894-50461913 Msa0735310:CDS 40.0%
ACTTCATCACGAGCTTATAG+CGG + chr5_1:50462183-50462202 None:intergenic 40.0%
! TTCTACTGCAGTTGCTTTGT+TGG - chr5_1:50462506-50462525 Msa0735310:intron 40.0%
GCCTCAAATATGATTGCCTT+TGG - chr5_1:50462528-50462547 Msa0735310:intron 40.0%
!!! AAGAGGCGTTTTCTTATGTG+GGG - chr5_1:50462729-50462748 Msa0735310:intron 40.0%
CAAAAAGATGGAGAGTCATG+TGG + chr5_1:50462758-50462777 None:intergenic 40.0%
! CACATGACTCTCCATCTTTT+TGG - chr5_1:50462756-50462775 Msa0735310:intron 40.0%
!! CTCCATCTTTTTGGCATCTA+TGG - chr5_1:50462765-50462784 Msa0735310:intron 40.0%
! GGCCACTTTCCTTTATATTG+TGG - chr5_1:50462816-50462835 Msa0735310:intron 40.0%
!! CCATTCAGGGCTTTAGAAAA+AGG + chr5_1:50462900-50462919 None:intergenic 40.0%
!!! CCTTTTTCTAAAGCCCTGAA+TGG - chr5_1:50462897-50462916 Msa0735310:intron 40.0%
!!! GATGGTTGTCATGCCATTTT+TGG - chr5_1:50463010-50463029 Msa0735310:intron 40.0%
!! GCCTTCTTTCTTGAGTTGAT+TGG - chr5_1:50463115-50463134 Msa0735310:intron 40.0%
CCCAATCAACTCAAGAAAGA+AGG + chr5_1:50463119-50463138 None:intergenic 40.0%
GGCACACTTACACTGATAAA+TGG - chr5_1:50463344-50463363 Msa0735310:intron 40.0%
TGCTCATATGATTGTGCACA+AGG + chr5_1:50463443-50463462 None:intergenic 40.0%
GCAAGAATAAAGATGGGGAT+AGG - chr5_1:50463460-50463479 Msa0735310:intron 40.0%
!! TCTAATGATGTAGCACTGGT+TGG - chr5_1:50463946-50463965 Msa0735310:CDS 40.0%
TAACACACGCGCAGAATCAA+AGG - chr5_1:50461615-50461634 Msa0735310:three_prime_UTR 45.0%
AACACACGCGCAGAATCAAA+GGG - chr5_1:50461616-50461635 Msa0735310:three_prime_UTR 45.0%
AAGAAAAGCAGAACGCGTCT+CGG - chr5_1:50461732-50461751 Msa0735310:three_prime_UTR 45.0%
CTGTTAGTGGAAGCAGTGAA+TGG - chr5_1:50461757-50461776 Msa0735310:three_prime_UTR 45.0%
TTAGTGGAAGCAGTGAATGG+CGG - chr5_1:50461760-50461779 Msa0735310:three_prime_UTR 45.0%
TGCAGTAGAAAACCTCCTTG+GGG + chr5_1:50462496-50462515 None:intergenic 45.0%
CTGCAGTAGAAAACCTCCTT+GGG + chr5_1:50462497-50462516 None:intergenic 45.0%
ACTGCAGTAGAAAACCTCCT+TGG + chr5_1:50462498-50462517 None:intergenic 45.0%
!!! AGAGGCGTTTTCTTATGTGG+GGG - chr5_1:50462730-50462749 Msa0735310:intron 45.0%
GCCCATAGATGCCAAAAAGA+TGG + chr5_1:50462770-50462789 None:intergenic 45.0%
TGATCTTGGCTGCAACATCA+TGG + chr5_1:50462867-50462886 None:intergenic 45.0%
TTCACCAAGAGTGATCTTGC+AGG - chr5_1:50463717-50463736 Msa0735310:CDS 45.0%
GGCTTCTAATGATGTAGCAC+TGG - chr5_1:50463942-50463961 Msa0735310:CDS 45.0%
!! TGTAGCACTGGTTGGTGATT+TGG - chr5_1:50463954-50463973 Msa0735310:CDS 45.0%
ACGCGCAGAATCAAAGGGAT+TGG - chr5_1:50461621-50461640 Msa0735310:three_prime_UTR 50.0%
GCGTCTACAACTGGACGTTT+GGG - chr5_1:50462350-50462369 Msa0735310:intron 50.0%
CATCTAGAGAGATGGTTGCG+TGG - chr5_1:50462572-50462591 Msa0735310:intron 50.0%
! AGACCGTGTTGCTACTATCG+CGG - chr5_1:50462795-50462814 Msa0735310:intron 50.0%
GCAACCTGCAAGATCACTCT+TGG + chr5_1:50463724-50463743 None:intergenic 50.0%
ACGCGTCTCGGATCTGTTAG+TGG - chr5_1:50461744-50461763 Msa0735310:three_prime_UTR 55.0%
TAGCCCTTCGCGTCTACAAC+TGG - chr5_1:50462341-50462360 Msa0735310:intron 55.0%
CGTCCAGTTGTAGACGCGAA+GGG + chr5_1:50462347-50462366 None:intergenic 55.0%
ACGTCCAGTTGTAGACGCGA+AGG + chr5_1:50462348-50462367 None:intergenic 55.0%
CGCGTCTACAACTGGACGTT+TGG - chr5_1:50462349-50462368 Msa0735310:intron 55.0%
! GACGTTTGGGAGCGTGAACT+GGG - chr5_1:50462363-50462382 Msa0735310:intron 55.0%
TGGCCGCGATAGTAGCAACA+CGG + chr5_1:50462801-50462820 None:intergenic 55.0%
! GGACGTTTGGGAGCGTGAAC+TGG - chr5_1:50462362-50462381 Msa0735310:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 50461553 50464058 50461553 ID=Msa0735310;Name=Msa0735310
chr5_1 mRNA 50461553 50464058 50461553 ID=Msa0735310-mRNA-1;Parent=Msa0735310;Name=Msa0735310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=45|1|1|1|1|1|2|328|135
chr5_1 exon 50461553 50461970 50461553 ID=Msa0735310-mRNA-1:exon:14224;Parent=Msa0735310-mRNA-1
chr5_1 exon 50463696 50464058 50463696 ID=Msa0735310-mRNA-1:exon:14223;Parent=Msa0735310-mRNA-1
chr5_1 five_prime_UTR 50464014 50464058 50464014 ID=Msa0735310-mRNA-1:five_prime_utr;Parent=Msa0735310-mRNA-1
chr5_1 CDS 50463696 50464013 50463696 ID=Msa0735310-mRNA-1:cds;Parent=Msa0735310-mRNA-1
chr5_1 CDS 50461881 50461970 50461881 ID=Msa0735310-mRNA-1:cds;Parent=Msa0735310-mRNA-1
chr5_1 three_prime_UTR 50461553 50461880 50461553 ID=Msa0735310-mRNA-1:three_prime_utr;Parent=Msa0735310-mRNA-1
Gene Sequence

>Msa0735310

ATGGAAGAACGCGATGTTAACACACGCGCAGAATCAAAGGGATTGGAAGATTCATTACAAAACCTAATTCACATTCATCATCAAAAATCTCTCATTCTCACCAATCAAACCGAAAAAGCGAAGAAAGATGCAATAAGAAAAGCAGAACGCGTCTCGGATCTGTTAGTGGAAGCAGTGAATGGCGGTGTTCAAGATTCGTTTATTTATCAGAAGAGAATTGAGCTTGAAATTCGTGCTCTTGCTGTAACTATTTCGAGATTTGTGAAACAAACGGATCAGTGGCTTAATGCTACTCATTCTCTTAACACTGCTCTCAAGGAAATTGGAGACTTTGAGAATTGGATGAAGATCATGGAATACGATTTTAAAAGTATTAATGCAGCTATACAAAACATTCACCAAGAGTGA

Protein sequence

>Msa0735310

MEERDVNTRAESKGLEDSLQNLIHIHHQKSLILTNQTEKAKKDAIRKAERVSDLLVEAVNGGVQDSFIYQKRIELEIRALAVTISRFVKQTDQWLNATHSLNTALKEIGDFENWMKIMEYDFKSINAAIQNIHQE*