AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0318790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318790 MtrunA17_Chr2g0292681 90.016 621 20 5 1 583 1 617 0.0 1098
Msa0318790 MtrunA17_Chr2g0292671 92.638 163 12 0 117 279 2 164 4.91e-108 322
Msa0318790 MtrunA17_Chr8g0371521 55.385 260 111 2 22 276 6 265 3.95e-93 294
Msa0318790 MtrunA17_Chr7g0237581 55.508 236 102 1 44 279 20 252 3.60e-86 270
Msa0318790 MtrunA17_Chr7g0237621 54.772 241 106 1 44 284 32 269 4.16e-85 267
Msa0318790 MtrunA17_Chr2g0292661 84.906 106 10 2 1 103 1 103 1.72e-54 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318790 AT4G20900.2 54.610 282 122 3 2 279 4 283 4.99e-102 316
Msa0318790 AT4G20900.1 51.678 298 122 4 2 279 4 299 9.69e-98 305
Msa0318790 AT3G51280.1 55.336 253 112 1 25 276 7 259 6.36e-89 281
Msa0318790 AT5G44330.1 51.292 271 126 2 5 275 7 271 1.56e-84 271
Msa0318790 AT5G48850.1 58.621 203 84 0 45 247 24 226 1.17e-82 261
Msa0318790 AT1G04770.1 56.808 213 86 1 32 244 10 216 4.78e-76 244
Msa0318790 AT5G22794.2 41.667 72 42 0 120 191 66 137 9.96e-12 65.9

Find 120 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTATACTACCTTGATTT+TGG 0.156762 2_4:-17939164 None:intergenic
ACAATACTCCACACCGAATT+TGG 0.197802 2_4:+17938672 Msa0318790:CDS
CTCCTCGCACTGACACAAAT+TGG 0.214620 2_4:+17938977 Msa0318790:CDS
GATTTCTTTCTCTCAGAAAA+TGG 0.226099 2_4:-17937079 None:intergenic
GTTATTCATAAGGTTCCTTC+TGG 0.250721 2_4:+17937139 Msa0318790:CDS
AAACTGCTGAAGAGCATTAT+AGG 0.255745 2_4:+17938329 Msa0318790:CDS
AGTTCATAAATTCAAGATTC+TGG 0.264962 2_4:-17939454 None:intergenic
AACAGAAGCTGCAAATTCTT+TGG 0.276602 2_4:-17939130 None:intergenic
TAGGAACAAACAGTGCAATT+TGG 0.304689 2_4:+17938474 Msa0318790:CDS
GAAGAAGAGCTGGGCGGATA+TGG 0.319137 2_4:+17939280 Msa0318790:CDS
CATCTTGGTTGAACTCTATA+AGG 0.323260 2_4:+17937735 Msa0318790:CDS
AACCCATTCTGTGTTGAGTT+TGG 0.324775 2_4:-17938952 None:intergenic
GCTTGGGCCTACTTGCAAAA+AGG 0.327423 2_4:+17938298 Msa0318790:CDS
TGTTAAAGCCAAACAGGTTC+AGG 0.331268 2_4:+17937174 Msa0318790:CDS
ATGAGAATGATGAGATTCTT+TGG 0.333088 2_4:-17938767 None:intergenic
CCATTTGAGCTCTCCAAATT+CGG 0.336612 2_4:-17938685 None:intergenic
TCGAAGCGATCAAGTCTTTC+CGG 0.345468 2_4:+17937674 Msa0318790:CDS
CGAGAGTGCCTTGAAGGATA+TGG 0.372126 2_4:+17937612 Msa0318790:CDS
AACAAGGAGAGCCAGGTTGC+AGG 0.377318 2_4:+17939604 Msa0318790:CDS
CCGAATTTGGAGAGCTCAAA+TGG 0.379534 2_4:+17938685 Msa0318790:CDS
ACCTGTTTGGCTTTAACATA+AGG 0.380773 2_4:-17937169 None:intergenic
CAAGAGATATCAAGGATACT+TGG 0.381295 2_4:+17938268 Msa0318790:CDS
ATGTAAGGAGCCTTCTCATT+AGG 0.388722 2_4:-17938922 None:intergenic
TGTATTTACCTGAACCTGTT+TGG 0.388891 2_4:-17937182 None:intergenic
CACTGTCCTTTGAGGTTGAT+AGG 0.391070 2_4:+17938455 Msa0318790:CDS
TTCTGGGCTGCCATCAATGC+TGG 0.391601 2_4:+17937580 Msa0318790:CDS
GACGCGATCACCAGCATTGA+TGG 0.392146 2_4:-17937590 None:intergenic
CAGAAAAGCACTGTCCTTTG+AGG 0.398933 2_4:+17938447 Msa0318790:intron
TGGTTTAAGGTTTCAGGATG+AGG 0.402812 2_4:-17939645 None:intergenic
TCCTTATGTTAAAGCCAAAC+AGG 0.403801 2_4:+17937168 Msa0318790:CDS
CATTGATGGCAGCCCAGAAA+AGG 0.416287 2_4:-17937576 None:intergenic
GGATACTTGGGAACTTGGCT+TGG 0.418538 2_4:+17938281 Msa0318790:CDS
GAGTTTGGCTTAATAATGTA+AGG 0.419019 2_4:-17938937 None:intergenic
TCGCGTCGAGAGTGCCTTGA+AGG 0.421679 2_4:+17937606 Msa0318790:CDS
AACTAAAGCCATATCCTTCA+AGG 0.422487 2_4:-17937620 None:intergenic
ATCAAGGATACTTGGGAACT+TGG 0.426919 2_4:+17938276 Msa0318790:CDS
AATGAAAGGGATGTATTCTC+TGG 0.431775 2_4:+17939569 Msa0318790:CDS
TTGTTATTGTTGTAGAGGTC+AGG 0.432018 2_4:+17938118 Msa0318790:intron
CAGTTAGTGGATAAAGATCC+AGG 0.435700 2_4:+17937541 Msa0318790:intron
TCTCTGCCCTTCTGATTCTC+AGG 0.443464 2_4:+17937699 Msa0318790:CDS
ACTATTGCAGCATCACCTTC+CGG 0.444919 2_4:-17939012 None:intergenic
TTAGGATAACTCACAAATGA+AGG 0.450079 2_4:-17938904 None:intergenic
GGAATCTCTTGACAACATCT+TGG 0.450411 2_4:+17937720 Msa0318790:CDS
AATCAGAAGGGCAGAGATGC+CGG 0.455923 2_4:-17937693 None:intergenic
AGAAGCGAACAAGGAGAGCC+AGG 0.461543 2_4:+17939597 Msa0318790:CDS
TAGTTATGAAGCAGTTGAAC+CGG 0.464179 2_4:+17937638 Msa0318790:CDS
AGAGTAGGACAGAAAATAAC+CGG 0.464589 2_4:+17938728 Msa0318790:CDS
TTATTCATAAGGTTCCTTCT+GGG 0.467739 2_4:+17937140 Msa0318790:CDS
GATACTTGGGAACTTGGCTT+GGG 0.467740 2_4:+17938282 Msa0318790:CDS
AGCCAAACTCAACACAGAAT+GGG 0.473682 2_4:+17938950 Msa0318790:CDS
AAAAGGGAAATTGCTTTACC+TGG 0.474611 2_4:-17937559 None:intergenic
TTTGTGTAAGGAGGAACCTT+CGG 0.474736 2_4:-17938835 None:intergenic
AAGCCAAACTCAACACAGAA+TGG 0.476640 2_4:+17938949 Msa0318790:CDS
GAATTTATGAACTTGAAAGA+TGG 0.482380 2_4:+17939464 Msa0318790:CDS
CAACAAATCTCATACAAAGT+TGG 0.489792 2_4:+17939101 Msa0318790:CDS
AACTGGCGAAGAAGAAGAGC+TGG 0.492530 2_4:+17939270 Msa0318790:CDS
AGAGTCATGTAGCATCTTGC+CGG 0.497445 2_4:-17937013 None:intergenic
CTGCACCTGTGAATGATACT+TGG 0.504642 2_4:+17939495 Msa0318790:CDS
ATAGCTGCTTCTGTGTTCAA+AGG 0.511003 2_4:-17939057 None:intergenic
GTGCTGAACAAGAGATATCA+AGG 0.512190 2_4:+17938260 Msa0318790:CDS
ATTGATGGCAGCCCAGAAAA+GGG 0.512233 2_4:-17937575 None:intergenic
CTGGAGAGGAGAAGCGAACA+AGG 0.513061 2_4:+17939588 Msa0318790:CDS
GAAGATGGTATGACATATGT+TGG 0.513644 2_4:+17938190 Msa0318790:CDS
AGAAGCAAGATTTCTACTTC+AGG 0.516971 2_4:+17938528 Msa0318790:CDS
TCTTTATTCACAGAAGCTGC+TGG 0.518402 2_4:-17937103 None:intergenic
GACGTTTGAAAGAAACTCTC+CGG 0.524804 2_4:+17936994 Msa0318790:CDS
CTCAACCAAGTATCATTCAC+AGG 0.525119 2_4:-17939500 None:intergenic
CTGCGGAAATGACAAATGAA+AGG 0.532212 2_4:+17939555 Msa0318790:CDS
TATTCATAAGGTTCCTTCTG+GGG 0.538359 2_4:+17937141 Msa0318790:CDS
AATCTCATCATTCTCATGCT+AGG 0.540394 2_4:+17938773 Msa0318790:CDS
AACAACGATCGCCTTACAGT+TGG 0.540417 2_4:-17939527 None:intergenic
TGCGGAAATGACAAATGAAA+GGG 0.540698 2_4:+17939556 Msa0318790:CDS
ATTGTTGTAGAGGTCAGGAA+GGG 0.542418 2_4:+17938123 Msa0318790:intron
GATACAAAAGACCAAAATCA+AGG 0.543998 2_4:+17939153 Msa0318790:CDS
GGTTGAGAAATCCAACTGTA+AGG 0.546675 2_4:+17939516 Msa0318790:CDS
GCAAAACATGCAAGATCTCA+AGG 0.551519 2_4:+17938220 Msa0318790:CDS
ATGGAGTCAACAGCACCACC+AGG 0.552571 2_4:+17938634 Msa0318790:CDS
TATTGTTGTAGAGGTCAGGA+AGG 0.553264 2_4:+17938122 Msa0318790:intron
AAGAGATATCAAGGATACTT+GGG 0.565042 2_4:+17938269 Msa0318790:CDS
TCAGAAAATGGAGAACGTAC+AGG 0.566918 2_4:-17937067 None:intergenic
CTTCGATTGCCTCGTCAGAC+CGG 0.567004 2_4:-17937657 None:intergenic
TCGTAATGTGACCGACGAGA+AGG 0.567568 2_4:-17939416 None:intergenic
CTGACACAAATTGGAGAACA+AGG 0.572861 2_4:+17938986 Msa0318790:CDS
CTCCAATTTGTGTCAGTGCG+AGG 0.574309 2_4:-17938979 None:intergenic
TAATGTGACCGACGAGAAGG+AGG 0.575381 2_4:-17939413 None:intergenic
TGTGATGACATATTCCTCCC+TGG 0.576964 2_4:-17938652 None:intergenic
AAATGGAAAGACCACTGATA+CGG 0.580779 2_4:+17938702 Msa0318790:CDS
AAGTTGAAGCAAATTGAAGA+TGG 0.583679 2_4:+17938175 Msa0318790:CDS
TACACAAATGCTGCAAGAGA+TGG 0.588606 2_4:+17938615 Msa0318790:CDS
TTCATTTGTCATTTCCGCAG+AGG 0.590486 2_4:-17939552 None:intergenic
TGTGAGTTATCCTAATGAGA+AGG 0.593270 2_4:+17938912 Msa0318790:CDS
TGCCGTCATCATCGTCGTCG+TGG 0.594135 2_4:+17937035 Msa0318790:CDS
TCCTGCAAAATCCGATCCGA+AGG 0.595239 2_4:+17938819 Msa0318790:CDS
TTTAAGGTTTCAGGATGAGG+AGG 0.597069 2_4:-17939642 None:intergenic
AGAGATTCCTGAGAATCAGA+AGG 0.602703 2_4:-17937706 None:intergenic
TGGCATCAGATGTCAAAGAC+CGG 0.602945 2_4:-17938747 None:intergenic
ATGGTATGACATATGTTGGA+AGG 0.607080 2_4:+17938194 Msa0318790:CDS
TTCCACGACGACGATGATGA+CGG 0.617914 2_4:-17937037 None:intergenic
GAGTATGAATTATTCTACAA+AGG 0.620440 2_4:+17939326 Msa0318790:CDS
TTTGTTCCTATCAACCTCAA+AGG 0.625304 2_4:-17938461 None:intergenic
CCACTGATACGGTTAAGAGT+AGG 0.632752 2_4:+17938713 Msa0318790:CDS
GCAGTTGAACCGGTCTGACG+AGG 0.636634 2_4:+17937648 Msa0318790:CDS
AAGGGATGTATTCTCTGGAG+AGG 0.640670 2_4:+17939574 Msa0318790:CDS
TGGAGAACAAGGACACCGGA+AGG 0.640937 2_4:+17938997 Msa0318790:CDS
TCTATCAGCCTCCTTCTCGT+CGG 0.644536 2_4:+17939405 Msa0318790:CDS
CAAAACATGCAAGATCTCAA+GGG 0.644877 2_4:+17938221 Msa0318790:CDS
GAGATTCCTGAGAATCAGAA+GGG 0.645235 2_4:-17937705 None:intergenic
ACATAAGGAGAATCCCCAGA+AGG 0.655272 2_4:-17937154 None:intergenic
ACTGGCGAAGAAGAAGAGCT+GGG 0.662743 2_4:+17939271 Msa0318790:CDS
GTAAGGAGGAACCTTCGGAT+CGG 0.666757 2_4:-17938830 None:intergenic
TGACTGTTGAAACCCCATGA+AGG 0.674237 2_4:-17938868 None:intergenic
TCTACTTCAGGCAGTAACCG+CGG 0.674832 2_4:+17938540 Msa0318790:CDS
AAATTGGAGAACAAGGACAC+CGG 0.677409 2_4:+17938993 Msa0318790:CDS
GCGATCGTTGTTTACCTCTG+CGG 0.681413 2_4:+17939538 Msa0318790:CDS
TGAGCTCTCCAAATTCGGTG+TGG 0.685828 2_4:-17938680 None:intergenic
CCTACTCTTAACCGTATCAG+TGG 0.687210 2_4:-17938713 None:intergenic
TGGAGTCAACAGCACCACCA+GGG 0.710851 2_4:+17938635 Msa0318790:CDS
GATGACATATTCCTCCCTGG+TGG 0.743091 2_4:-17938649 None:intergenic
GGCGAAGAAGAAGAGCTGGG+CGG 0.748473 2_4:+17939274 Msa0318790:CDS
AGTCAACAGCACCACCAGGG+AGG 0.755887 2_4:+17938638 Msa0318790:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCTTTGA+TGG + chr2_4:17937340-17937359 Msa0318790:intron 15.0%
!!! AATTTTTTTCTTTCTTTGAT+GGG + chr2_4:17937341-17937360 Msa0318790:intron 15.0%
!! ATTTGAATAAACTTACAAAA+AGG + chr2_4:17937895-17937914 Msa0318790:intron 15.0%
!!! ATTATTACTTTTCTCTTTAT+AGG - chr2_4:17938406-17938425 None:intergenic 15.0%
!!! TCTTTTTCATGTTATTCATA+AGG + chr2_4:17937129-17937148 Msa0318790:CDS 20.0%
!! ACAATCAAAATAGAAACTTT+AGG - chr2_4:17937220-17937239 None:intergenic 20.0%
!!! TATCAGTTAATTTTCTTTCT+TGG + chr2_4:17937483-17937502 Msa0318790:intron 20.0%
!! GAGATAATTAACTAACATAA+AGG - chr2_4:17937800-17937819 None:intergenic 20.0%
!! CTAAATGTTACAAATTAACA+GGG - chr2_4:17937942-17937961 None:intergenic 20.0%
!! ACTAACAGTAGTATTTAAAA+TGG - chr2_4:17939192-17939211 None:intergenic 20.0%
!!! TTTTGATTGTTTTTGTCTTC+TGG + chr2_4:17937230-17937249 Msa0318790:intron 25.0%
!!! TTTGATTGTTTTTGTCTTCT+GGG + chr2_4:17937231-17937250 Msa0318790:intron 25.0%
! AATCAAAATACAAACAGCTA+AGG - chr2_4:17937872-17937891 None:intergenic 25.0%
! TGAATAAACTTACAAAAAGG+TGG + chr2_4:17937898-17937917 Msa0318790:intron 25.0%
! GAATAAACTTACAAAAAGGT+GGG + chr2_4:17937899-17937918 Msa0318790:intron 25.0%
! CCTAAATGTTACAAATTAAC+AGG - chr2_4:17937943-17937962 None:intergenic 25.0%
!!! CCTGTTAATTTGTAACATTT+AGG + chr2_4:17937940-17937959 Msa0318790:intron 25.0%
! ATTTAGGTGTATCATTAAGT+AGG + chr2_4:17937956-17937975 Msa0318790:intron 25.0%
!! GTTTCCTATTTTATTGTTGA+AGG + chr2_4:17938060-17938079 Msa0318790:intron 25.0%
! GTAACCTTCAACAATAAAAT+AGG - chr2_4:17938067-17938086 None:intergenic 25.0%
! CATGTATAAACCATTAATGT+TGG - chr2_4:17938094-17938113 None:intergenic 25.0%
!! GTATATTGTTATTGTTGTAG+AGG + chr2_4:17938113-17938132 Msa0318790:intron 25.0%
!!! CATTTTGTTTCCATCTAAAT+AGG - chr2_4:17938361-17938380 None:intergenic 25.0%
!! ATTCTATACTACCTTGATTT+TGG - chr2_4:17939167-17939186 None:intergenic 25.0%
! AACTTGATTAACAATCTTCA+TGG + chr2_4:17939233-17939252 Msa0318790:intron 25.0%
! GAGTATGAATTATTCTACAA+AGG + chr2_4:17939326-17939345 Msa0318790:CDS 25.0%
! AGTTCATAAATTCAAGATTC+TGG - chr2_4:17939457-17939476 None:intergenic 25.0%
! GAATTTATGAACTTGAAAGA+TGG + chr2_4:17939464-17939483 Msa0318790:CDS 25.0%
GATTTCTTTCTCTCAGAAAA+TGG - chr2_4:17937082-17937101 None:intergenic 30.0%
TTATTCATAAGGTTCCTTCT+GGG + chr2_4:17937140-17937159 Msa0318790:CDS 30.0%
AATACAAGACCAAGAATTCA+AGG - chr2_4:17937279-17937298 None:intergenic 30.0%
!! TATCGTTTTGATAGAGTTTG+AGG + chr2_4:17937371-17937390 Msa0318790:intron 30.0%
!! ATCGTTTTGATAGAGTTTGA+GGG + chr2_4:17937372-17937391 Msa0318790:intron 30.0%
AACTGAGAGTTCTATTCTAA+AGG - chr2_4:17937422-17937441 None:intergenic 30.0%
TCTGAATTCTTTACAGTTAG+TGG + chr2_4:17937528-17937547 Msa0318790:intron 30.0%
! TAAAGCAATTTCCCTTTTCT+GGG + chr2_4:17937564-17937583 Msa0318790:CDS 30.0%
AGATTAGTATCTCGTATTGT+AGG + chr2_4:17938020-17938039 Msa0318790:intron 30.0%
AAGTTGAAGCAAATTGAAGA+TGG + chr2_4:17938175-17938194 Msa0318790:CDS 30.0%
AAGAGATATCAAGGATACTT+GGG + chr2_4:17938269-17938288 Msa0318790:CDS 30.0%
TAGGTAAACTCCTATTTAGA+TGG + chr2_4:17938348-17938367 Msa0318790:intron 30.0%
CTGCATAAACAAAACAAACA+AGG - chr2_4:17938430-17938449 None:intergenic 30.0%
ATGAGAATGATGAGATTCTT+TGG - chr2_4:17938770-17938789 None:intergenic 30.0%
AAAACCAAAAAGACCTTCAT+GGG + chr2_4:17938855-17938874 Msa0318790:CDS 30.0%
TTAGGATAACTCACAAATGA+AGG - chr2_4:17938907-17938926 None:intergenic 30.0%
! GAGTTTGGCTTAATAATGTA+AGG - chr2_4:17938940-17938959 None:intergenic 30.0%
CAACAAATCTCATACAAAGT+TGG + chr2_4:17939101-17939120 Msa0318790:CDS 30.0%
GATACAAAAGACCAAAATCA+AGG + chr2_4:17939153-17939172 Msa0318790:CDS 30.0%
GTTATTCATAAGGTTCCTTC+TGG + chr2_4:17937139-17937158 Msa0318790:CDS 35.0%
TATTCATAAGGTTCCTTCTG+GGG + chr2_4:17937141-17937160 Msa0318790:CDS 35.0%
TCCTTATGTTAAAGCCAAAC+AGG + chr2_4:17937168-17937187 Msa0318790:CDS 35.0%
!! ACCTGTTTGGCTTTAACATA+AGG - chr2_4:17937172-17937191 None:intergenic 35.0%
TGTATTTACCTGAACCTGTT+TGG - chr2_4:17937185-17937204 None:intergenic 35.0%
!! TCGTTTTGATAGAGTTTGAG+GGG + chr2_4:17937373-17937392 Msa0318790:intron 35.0%
AAAAGGGAAATTGCTTTACC+TGG - chr2_4:17937562-17937581 None:intergenic 35.0%
! GTAAAGCAATTTCCCTTTTC+TGG + chr2_4:17937563-17937582 Msa0318790:CDS 35.0%
AACTAAAGCCATATCCTTCA+AGG - chr2_4:17937623-17937642 None:intergenic 35.0%
TAGTTATGAAGCAGTTGAAC+CGG + chr2_4:17937638-17937657 Msa0318790:CDS 35.0%
CATCTTGGTTGAACTCTATA+AGG + chr2_4:17937735-17937754 Msa0318790:CDS 35.0%
CATGCATTTGAATGATAAGC+TGG + chr2_4:17937822-17937841 Msa0318790:intron 35.0%
CTTACAAAAAGGTGGGAATT+TGG + chr2_4:17937906-17937925 Msa0318790:intron 35.0%
GGTTACGAAACCAACATTAA+TGG + chr2_4:17938081-17938100 Msa0318790:intron 35.0%
! TTGTTATTGTTGTAGAGGTC+AGG + chr2_4:17938118-17938137 Msa0318790:intron 35.0%
GAAGATGGTATGACATATGT+TGG + chr2_4:17938190-17938209 Msa0318790:CDS 35.0%
ATGGTATGACATATGTTGGA+AGG + chr2_4:17938194-17938213 Msa0318790:CDS 35.0%
CAAAACATGCAAGATCTCAA+GGG + chr2_4:17938221-17938240 Msa0318790:CDS 35.0%
CAAGAGATATCAAGGATACT+TGG + chr2_4:17938268-17938287 Msa0318790:CDS 35.0%
! ATAATCGCCTTTTTGCAAGT+AGG - chr2_4:17938308-17938327 None:intergenic 35.0%
!! AAACTGCTGAAGAGCATTAT+AGG + chr2_4:17938329-17938348 Msa0318790:CDS 35.0%
TTTGTTCCTATCAACCTCAA+AGG - chr2_4:17938464-17938483 None:intergenic 35.0%
TAGGAACAAACAGTGCAATT+TGG + chr2_4:17938474-17938493 Msa0318790:CDS 35.0%
AGAAGCAAGATTTCTACTTC+AGG + chr2_4:17938528-17938547 Msa0318790:CDS 35.0%
AAATGGAAAGACCACTGATA+CGG + chr2_4:17938702-17938721 Msa0318790:CDS 35.0%
AGAGTAGGACAGAAAATAAC+CGG + chr2_4:17938728-17938747 Msa0318790:CDS 35.0%
AATCTCATCATTCTCATGCT+AGG + chr2_4:17938773-17938792 Msa0318790:CDS 35.0%
!!! CTTTTTGGTTTTGTGTAAGG+AGG - chr2_4:17938847-17938866 None:intergenic 35.0%
!!! GGTCTTTTTGGTTTTGTGTA+AGG - chr2_4:17938850-17938869 None:intergenic 35.0%
CAAAACCAAAAAGACCTTCA+TGG + chr2_4:17938854-17938873 Msa0318790:CDS 35.0%
AAACCAAAAAGACCTTCATG+GGG + chr2_4:17938856-17938875 Msa0318790:CDS 35.0%
TGTGAGTTATCCTAATGAGA+AGG + chr2_4:17938912-17938931 Msa0318790:CDS 35.0%
AACAGAAGCTGCAAATTCTT+TGG - chr2_4:17939133-17939152 None:intergenic 35.0%
TGGATTTGCAGACAAAAAAC+TGG + chr2_4:17939253-17939272 Msa0318790:intron 35.0%
TGCGGAAATGACAAATGAAA+GGG + chr2_4:17939556-17939575 Msa0318790:CDS 35.0%
AATGAAAGGGATGTATTCTC+TGG + chr2_4:17939569-17939588 Msa0318790:CDS 35.0%
GACGTTTGAAAGAAACTCTC+CGG + chr2_4:17936994-17937013 Msa0318790:CDS 40.0%
TCAGAAAATGGAGAACGTAC+AGG - chr2_4:17937070-17937089 None:intergenic 40.0%
TCTTTATTCACAGAAGCTGC+TGG - chr2_4:17937106-17937125 None:intergenic 40.0%
TGTTAAAGCCAAACAGGTTC+AGG + chr2_4:17937174-17937193 Msa0318790:CDS 40.0%
! CTTCTGGGTTGTGATGTTTT+TGG + chr2_4:17937246-17937265 Msa0318790:intron 40.0%
!!! TGGGTTGTGATGTTTTTGGT+TGG + chr2_4:17937250-17937269 Msa0318790:intron 40.0%
!! GGTTGGTTTCCTTGAATTCT+TGG + chr2_4:17937267-17937286 Msa0318790:intron 40.0%
AGTTTGAGGGGTAGAATATG+TGG + chr2_4:17937385-17937404 Msa0318790:intron 40.0%
! CAGTTAGTGGATAAAGATCC+AGG + chr2_4:17937541-17937560 Msa0318790:intron 40.0%
GAGATTCCTGAGAATCAGAA+GGG - chr2_4:17937708-17937727 None:intergenic 40.0%
AGAGATTCCTGAGAATCAGA+AGG - chr2_4:17937709-17937728 None:intergenic 40.0%
GGAATCTCTTGACAACATCT+TGG + chr2_4:17937720-17937739 Msa0318790:CDS 40.0%
TATTGTTGTAGAGGTCAGGA+AGG + chr2_4:17938122-17938141 Msa0318790:intron 40.0%
ATTGTTGTAGAGGTCAGGAA+GGG + chr2_4:17938123-17938142 Msa0318790:intron 40.0%
GCAAAACATGCAAGATCTCA+AGG + chr2_4:17938220-17938239 Msa0318790:CDS 40.0%
GTGCTGAACAAGAGATATCA+AGG + chr2_4:17938260-17938279 Msa0318790:CDS 40.0%
ATCAAGGATACTTGGGAACT+TGG + chr2_4:17938276-17938295 Msa0318790:CDS 40.0%
GGCGACAAAAAACAGAAAGA+TGG + chr2_4:17938561-17938580 Msa0318790:CDS 40.0%
TACACAAATGCTGCAAGAGA+TGG + chr2_4:17938615-17938634 Msa0318790:CDS 40.0%
ACAATACTCCACACCGAATT+TGG + chr2_4:17938672-17938691 Msa0318790:CDS 40.0%
CCATTTGAGCTCTCCAAATT+CGG - chr2_4:17938688-17938707 None:intergenic 40.0%
TTTGTGTAAGGAGGAACCTT+CGG - chr2_4:17938838-17938857 None:intergenic 40.0%
! AAACCCCATGAAGGTCTTTT+TGG - chr2_4:17938862-17938881 None:intergenic 40.0%
ATGTAAGGAGCCTTCTCATT+AGG - chr2_4:17938925-17938944 None:intergenic 40.0%
AAGCCAAACTCAACACAGAA+TGG + chr2_4:17938949-17938968 Msa0318790:CDS 40.0%
AGCCAAACTCAACACAGAAT+GGG + chr2_4:17938950-17938969 Msa0318790:CDS 40.0%
AACCCATTCTGTGTTGAGTT+TGG - chr2_4:17938955-17938974 None:intergenic 40.0%
CTGACACAAATTGGAGAACA+AGG + chr2_4:17938986-17939005 Msa0318790:CDS 40.0%
AAATTGGAGAACAAGGACAC+CGG + chr2_4:17938993-17939012 Msa0318790:CDS 40.0%
! GGTGATGCTGCAATAGTTTT+TGG + chr2_4:17939018-17939037 Msa0318790:CDS 40.0%
!!! GCTGCAATAGTTTTTGGTTC+TGG + chr2_4:17939024-17939043 Msa0318790:CDS 40.0%
ATAGCTGCTTCTGTGTTCAA+AGG - chr2_4:17939060-17939079 None:intergenic 40.0%
CTCAACCAAGTATCATTCAC+AGG - chr2_4:17939503-17939522 None:intergenic 40.0%
GGTTGAGAAATCCAACTGTA+AGG + chr2_4:17939516-17939535 Msa0318790:CDS 40.0%
! TTCATTTGTCATTTCCGCAG+AGG - chr2_4:17939555-17939574 None:intergenic 40.0%
CTGCGGAAATGACAAATGAA+AGG + chr2_4:17939555-17939574 Msa0318790:CDS 40.0%
AGAGTCATGTAGCATCTTGC+CGG - chr2_4:17937016-17937035 None:intergenic 45.0%
ACATAAGGAGAATCCCCAGA+AGG - chr2_4:17937157-17937176 None:intergenic 45.0%
! ATTGATGGCAGCCCAGAAAA+GGG - chr2_4:17937578-17937597 None:intergenic 45.0%
TCGAAGCGATCAAGTCTTTC+CGG + chr2_4:17937674-17937693 Msa0318790:CDS 45.0%
GATACTTGGGAACTTGGCTT+GGG + chr2_4:17938282-17938301 Msa0318790:CDS 45.0%
!! CAGAAAAGCACTGTCCTTTG+AGG + chr2_4:17938447-17938466 Msa0318790:intron 45.0%
!! CACTGTCCTTTGAGGTTGAT+AGG + chr2_4:17938455-17938474 Msa0318790:CDS 45.0%
!!! CTTTCTGTTTTTTGTCGCCG+CGG - chr2_4:17938560-17938579 None:intergenic 45.0%
TGTGATGACATATTCCTCCC+TGG - chr2_4:17938655-17938674 None:intergenic 45.0%
CCGAATTTGGAGAGCTCAAA+TGG + chr2_4:17938685-17938704 Msa0318790:CDS 45.0%
CCTACTCTTAACCGTATCAG+TGG - chr2_4:17938716-17938735 None:intergenic 45.0%
CCACTGATACGGTTAAGAGT+AGG + chr2_4:17938713-17938732 Msa0318790:CDS 45.0%
TGGCATCAGATGTCAAAGAC+CGG - chr2_4:17938750-17938769 None:intergenic 45.0%
TGACTGTTGAAACCCCATGA+AGG - chr2_4:17938871-17938890 None:intergenic 45.0%
ACTATTGCAGCATCACCTTC+CGG - chr2_4:17939015-17939034 None:intergenic 45.0%
CTGCACCTGTGAATGATACT+TGG + chr2_4:17939495-17939514 Msa0318790:CDS 45.0%
AACAACGATCGCCTTACAGT+TGG - chr2_4:17939530-17939549 None:intergenic 45.0%
! AAGGGATGTATTCTCTGGAG+AGG + chr2_4:17939574-17939593 Msa0318790:CDS 45.0%
ATCCTGAAAAACCTGCAACC+TGG - chr2_4:17939618-17939637 None:intergenic 45.0%
TTCCACGACGACGATGATGA+CGG - chr2_4:17937040-17937059 None:intergenic 50.0%
! CATTGATGGCAGCCCAGAAA+AGG - chr2_4:17937579-17937598 None:intergenic 50.0%
!! CGAGAGTGCCTTGAAGGATA+TGG + chr2_4:17937612-17937631 Msa0318790:CDS 50.0%
AATCAGAAGGGCAGAGATGC+CGG - chr2_4:17937696-17937715 None:intergenic 50.0%
TCTCTGCCCTTCTGATTCTC+AGG + chr2_4:17937699-17937718 Msa0318790:CDS 50.0%
GGATACTTGGGAACTTGGCT+TGG + chr2_4:17938281-17938300 Msa0318790:CDS 50.0%
GCTTGGGCCTACTTGCAAAA+AGG + chr2_4:17938298-17938317 Msa0318790:CDS 50.0%
TCTACTTCAGGCAGTAACCG+CGG + chr2_4:17938540-17938559 Msa0318790:CDS 50.0%
GATGACATATTCCTCCCTGG+TGG - chr2_4:17938652-17938671 None:intergenic 50.0%
! TGAGCTCTCCAAATTCGGTG+TGG - chr2_4:17938683-17938702 None:intergenic 50.0%
TCCTGCAAAATCCGATCCGA+AGG + chr2_4:17938819-17938838 Msa0318790:CDS 50.0%
! ACCTTCGGATCGGATTTTGC+AGG - chr2_4:17938823-17938842 None:intergenic 50.0%
GTAAGGAGGAACCTTCGGAT+CGG - chr2_4:17938833-17938852 None:intergenic 50.0%
! CTCCTCGCACTGACACAAAT+TGG + chr2_4:17938977-17938996 Msa0318790:CDS 50.0%
CTCCAATTTGTGTCAGTGCG+AGG - chr2_4:17938982-17939001 None:intergenic 50.0%
AACTGGCGAAGAAGAAGAGC+TGG + chr2_4:17939270-17939289 Msa0318790:CDS 50.0%
ACTGGCGAAGAAGAAGAGCT+GGG + chr2_4:17939271-17939290 Msa0318790:CDS 50.0%
TCTATCAGCCTCCTTCTCGT+CGG + chr2_4:17939405-17939424 Msa0318790:CDS 50.0%
TAATGTGACCGACGAGAAGG+AGG - chr2_4:17939416-17939435 None:intergenic 50.0%
TCGTAATGTGACCGACGAGA+AGG - chr2_4:17939419-17939438 None:intergenic 50.0%
GCGATCGTTGTTTACCTCTG+CGG + chr2_4:17939538-17939557 Msa0318790:CDS 50.0%
! AGCCAGGTTGCAGGTTTTTC+AGG + chr2_4:17939613-17939632 Msa0318790:CDS 50.0%
TTCTGGGCTGCCATCAATGC+TGG + chr2_4:17937580-17937599 Msa0318790:CDS 55.0%
! GACGCGATCACCAGCATTGA+TGG - chr2_4:17937593-17937612 None:intergenic 55.0%
CTTCGATTGCCTCGTCAGAC+CGG - chr2_4:17937660-17937679 None:intergenic 55.0%
ATGGAGTCAACAGCACCACC+AGG + chr2_4:17938634-17938653 Msa0318790:CDS 55.0%
TGGAGTCAACAGCACCACCA+GGG + chr2_4:17938635-17938654 Msa0318790:CDS 55.0%
TGGAGAACAAGGACACCGGA+AGG + chr2_4:17938997-17939016 Msa0318790:CDS 55.0%
GAAGAAGAGCTGGGCGGATA+TGG + chr2_4:17939280-17939299 Msa0318790:CDS 55.0%
CTGGAGAGGAGAAGCGAACA+AGG + chr2_4:17939588-17939607 Msa0318790:CDS 55.0%
AGAAGCGAACAAGGAGAGCC+AGG + chr2_4:17939597-17939616 Msa0318790:CDS 55.0%
AACAAGGAGAGCCAGGTTGC+AGG + chr2_4:17939604-17939623 Msa0318790:CDS 55.0%
TGCCGTCATCATCGTCGTCG+TGG + chr2_4:17937035-17937054 Msa0318790:CDS 60.0%
!! TCGCGTCGAGAGTGCCTTGA+AGG + chr2_4:17937606-17937625 Msa0318790:CDS 60.0%
GCAGTTGAACCGGTCTGACG+AGG + chr2_4:17937648-17937667 Msa0318790:CDS 60.0%
AGTCAACAGCACCACCAGGG+AGG + chr2_4:17938638-17938657 Msa0318790:CDS 60.0%
GGCGAAGAAGAAGAGCTGGG+CGG + chr2_4:17939274-17939293 Msa0318790:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 17936992 17939655 17936992 ID=Msa0318790;Name=Msa0318790
chr2_4 mRNA 17936992 17939655 17936992 ID=Msa0318790-mRNA-1;Parent=Msa0318790;Name=Msa0318790-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.75|0.4|1|1|1|5|0|583
chr2_4 exon 17936992 17937195 17936992 ID=Msa0318790-mRNA-1:exon:6637;Parent=Msa0318790-mRNA-1
chr2_4 exon 17937544 17937756 17937544 ID=Msa0318790-mRNA-1:exon:6638;Parent=Msa0318790-mRNA-1
chr2_4 exon 17938133 17938350 17938133 ID=Msa0318790-mRNA-1:exon:6639;Parent=Msa0318790-mRNA-1
chr2_4 exon 17938450 17939174 17938450 ID=Msa0318790-mRNA-1:exon:6640;Parent=Msa0318790-mRNA-1
chr2_4 exon 17939264 17939655 17939264 ID=Msa0318790-mRNA-1:exon:6641;Parent=Msa0318790-mRNA-1
chr2_4 CDS 17936992 17937195 17936992 ID=Msa0318790-mRNA-1:cds;Parent=Msa0318790-mRNA-1
chr2_4 CDS 17937544 17937756 17937544 ID=Msa0318790-mRNA-1:cds;Parent=Msa0318790-mRNA-1
chr2_4 CDS 17938133 17938350 17938133 ID=Msa0318790-mRNA-1:cds;Parent=Msa0318790-mRNA-1
chr2_4 CDS 17938450 17939174 17938450 ID=Msa0318790-mRNA-1:cds;Parent=Msa0318790-mRNA-1
chr2_4 CDS 17939264 17939655 17939264 ID=Msa0318790-mRNA-1:cds;Parent=Msa0318790-mRNA-1
Gene Sequence

>Msa0318790

ATGACGTTTGAAAGAAACTCTCCGGCAAGATGCTACATGACTCTGCCGTCATCATCGTCGTCGTGGAAATCAAGACCTGTACGTTCTCCATTTTCTGAGAGAAAGAAATCACCAGCAGCTTCTGTGAATAAAGATGATCTTTTTCATGTTATTCATAAGGTTCCTTCTGGGGATTCTCCTTATGTTAAAGCCAAACAGGTTCAGTTAGTGGATAAAGATCCAGGTAAAGCAATTTCCCTTTTCTGGGCTGCCATCAATGCTGGTGATCGCGTCGAGAGTGCCTTGAAGGATATGGCTTTAGTTATGAAGCAGTTGAACCGGTCTGACGAGGCAATCGAAGCGATCAAGTCTTTCCGGCATCTCTGCCCTTCTGATTCTCAGGAATCTCTTGACAACATCTTGGTTGAACTCTATAAGAGGTCAGGAAGGGTTGATGAAGAGATTGACATGCTTCATCAAAAGTTGAAGCAAATTGAAGATGGTATGACATATGTTGGAAGGACAGCAAAACATGCAAGATCTCAAGGGAAGAAAGTTCAAATCAGTGCTGAACAAGAGATATCAAGGATACTTGGGAACTTGGCTTGGGCCTACTTGCAAAAAGGCGATTATAAAACTGCTGAAGAGCATTATAGAAAAGCACTGTCCTTTGAGGTTGATAGGAACAAACAGTGCAATTTGGCAATATGTTTGATGCAGATGAACAAGATTACAGAAGCAAGATTTCTACTTCAGGCAGTAACCGCGGCGACAAAAAACAGAAAGATGGACGATTCTTTCGTCAAGTCTTATGAACGCGCTACACAAATGCTGCAAGAGATGGAGTCAACAGCACCACCAGGGAGGAATATGTCATCACAATACTCCACACCGAATTTGGAGAGCTCAAATGGAAAGACCACTGATACGGTTAAGAGTAGGACAGAAAATAACCGGTCTTTGACATCTGATGCCAAAGAATCTCATCATTCTCATGCTAGGAGAAGATTATATGAGTCTGTTGATCCTGCAAAATCCGATCCGAAGGTTCCTCCTTACACAAAACCAAAAAGACCTTCATGGGGTTTCAACAGTCATTCAGATTCTAAACCTTCATTTGTGAGTTATCCTAATGAGAAGGCTCCTTACATTATTAAGCCAAACTCAACACAGAATGGGTTTTCTCCTCGCACTGACACAAATTGGAGAACAAGGACACCGGAAGGTGATGCTGCAATAGTTTTTGGTTCTGGTTCGATACATCCTTTGAACACAGAAGCAGCTATGAAATTCACAAAAAATGACAACAACAAATCTCATACAAAGTTGGATGTAACCAAAGAATTTGCAGCTTCTGTTGATACAAAAGACCAAAATCAAGACAAAAAACTGGCGAAGAAGAAGAGCTGGGCGGATATGGTAGAAGAAGAACAGCAATCTGAAGAGTATGAATTATTCTACAAAGGATATACAAATTTTGACGCTCAAGTGTTTCATAATGAAAATGAAAACTCAAACATAGTCTATCAGCCTCCTTCTCGTCGGTCACATTACGAAACTGAAAGTTTAAACCAGAATCTTGAATTTATGAACTTGAAAGATGGCTATAATGCTGCACCTGTGAATGATACTTGGTTGAGAAATCCAACTGTAAGGCGATCGTTGTTTACCTCTGCGGAAATGACAAATGAAAGGGATGTATTCTCTGGAGAGGAGAAGCGAACAAGGAGAGCCAGGTTGCAGGTTTTTCAGGATATTACCTCCTCATCCTGA
Protein sequence

>Msa0318790

MTFERNSPARCYMTLPSSSSSWKSRPVRSPFSERKKSPAASVNKDDLFHVIHKVPSGDSPYVKAKQVQLVDKDPGKAISLFWAAINAGDRVESALKDMALVMKQLNRSDEAIEAIKSFRHLCPSDSQESLDNILVELYKRSGRVDEEIDMLHQKLKQIEDGMTYVGRTAKHARSQGKKVQISAEQEISRILGNLAWAYLQKGDYKTAEEHYRKALSFEVDRNKQCNLAICLMQMNKITEARFLLQAVTAATKNRKMDDSFVKSYERATQMLQEMESTAPPGRNMSSQYSTPNLESSNGKTTDTVKSRTENNRSLTSDAKESHHSHARRRLYESVDPAKSDPKVPPYTKPKRPSWGFNSHSDSKPSFVSYPNEKAPYIIKPNSTQNGFSPRTDTNWRTRTPEGDAAIVFGSGSIHPLNTEAAMKFTKNDNNKSHTKLDVTKEFAASVDTKDQNQDKKLAKKKSWADMVEEEQQSEEYELFYKGYTNFDAQVFHNENENSNIVYQPPSRRSHYETESLNQNLEFMNLKDGYNAAPVNDTWLRNPTVRRSLFTSAEMTNERDVFSGEEKRTRRARLQVFQDITSSS*