Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0318910 | KAF4382395.1 | 48.980 | 343 | 97 | 5 | 474 | 751 | 277 | 606 | 2.65e-94 | 335 |
| Msa0318910 | KAF4382395.1 | 56.086 | 608 | 162 | 11 | 187 | 703 | 269 | 862 | 0.0 | 685 |
| Msa0318910 | KAF4382395.1 | 68.852 | 305 | 90 | 2 | 952 | 1253 | 269 | 571 | 9.98e-142 | 463 |
| Msa0318910 | KAF4382395.1 | 70.408 | 294 | 82 | 2 | 960 | 1250 | 570 | 861 | 5.23e-140 | 459 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0241410 | Msa0318910 | 0.823207 | 1.568454e-53 | -8.615850e-47 |
| Msa0279330 | Msa0318910 | 0.862188 | 6.047613e-64 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0318910 | MtrunA17_Chr2g0292821 | 93.603 | 297 | 19 | 0 | 958 | 1254 | 297 | 593 | 0.0 | 602 |
| Msa0318910 | MtrunA17_Chr2g0292821 | 63.889 | 288 | 42 | 3 | 474 | 699 | 299 | 586 | 9.71e-112 | 363 |
| Msa0318910 | MtrunA17_Chr2g0292821 | 81.132 | 53 | 10 | 0 | 458 | 510 | 20 | 72 | 5.97e-21 | 99.0 |
| Msa0318910 | MtrunA17_Chr2g0292801 | 92.593 | 297 | 22 | 0 | 958 | 1254 | 297 | 593 | 0.0 | 598 |
| Msa0318910 | MtrunA17_Chr2g0292801 | 64.236 | 288 | 41 | 3 | 474 | 699 | 299 | 586 | 1.53e-113 | 367 |
| Msa0318910 | MtrunA17_Chr2g0292801 | 81.132 | 53 | 10 | 0 | 458 | 510 | 20 | 72 | 6.13e-21 | 99.0 |
| Msa0318910 | MtrunA17_Chr2g0292811 | 85.085 | 295 | 44 | 0 | 960 | 1254 | 36 | 330 | 0.0 | 552 |
| Msa0318910 | MtrunA17_Chr2g0292811 | 76.389 | 288 | 45 | 3 | 195 | 459 | 36 | 323 | 1.78e-151 | 458 |
| Msa0318910 | MtrunA17_Chr2g0292811 | 69.688 | 320 | 35 | 3 | 442 | 699 | 4 | 323 | 5.38e-148 | 449 |
| Msa0318910 | MtrunA17_Chr2g0292811 | 69.565 | 69 | 21 | 0 | 1 | 69 | 1 | 69 | 1.29e-23 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0318910 | AT3G19430.2 | 59.347 | 337 | 110 | 5 | 924 | 1254 | 267 | 582 | 1.13e-131 | 416 |
| Msa0318910 | AT3G19430.2 | 55.518 | 299 | 100 | 7 | 184 | 453 | 275 | 569 | 2.11e-100 | 332 |
| Msa0318910 | AT3G19430.2 | 46.552 | 290 | 75 | 6 | 476 | 693 | 288 | 569 | 6.39e-72 | 252 |
| Msa0318910 | AT3G19430.2 | 57.746 | 71 | 25 | 2 | 440 | 510 | 9 | 74 | 4.18e-15 | 80.5 |
| Msa0318910 | AT3G19430.1 | 59.347 | 337 | 110 | 5 | 924 | 1254 | 244 | 559 | 1.34e-131 | 415 |
| Msa0318910 | AT3G19430.1 | 55.518 | 299 | 100 | 7 | 184 | 453 | 252 | 546 | 2.35e-100 | 331 |
| Msa0318910 | AT3G19430.1 | 46.503 | 286 | 81 | 5 | 476 | 693 | 265 | 546 | 5.81e-72 | 251 |
| Msa0318910 | AT3G19430.1 | 71.154 | 52 | 13 | 1 | 459 | 510 | 2 | 51 | 5.07e-14 | 77.0 |
| Msa0318910 | AT5G60520.1 | 46.442 | 267 | 123 | 4 | 977 | 1223 | 66 | 332 | 2.63e-74 | 251 |
| Msa0318910 | AT5G60520.1 | 40.824 | 267 | 115 | 7 | 212 | 435 | 66 | 332 | 5.17e-57 | 201 |
Find 308 sgRNAs with CRISPR-Local
Find 1398 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGCCAATAATGGGAGG+TGG | 0.559962 | 2_4:+18068922 | Msa0318910:CDS |
| AAAATGCCAATAATGGGAGG+TGG | 0.581006 | 2_4:+18078715 | Msa0318910:CDS |
| AAAATGCCAATAATGGGAGG+TGG | 0.581006 | 2_4:+18087914 | Msa0318910:CDS |
| AAACGTGCTACAGAACAATC+TGG | 0.425148 | 2_4:-18078769 | None:intergenic |
| AAATCTTAGGATAATTGCTA+AGG | 0.552424 | 2_4:+18078519 | Msa0318910:CDS |
| AACACAAATAGGTTTGCAAG+AGG | 0.662982 | 2_4:-18086300 | None:intergenic |
| AACGTGCTACAGAACAATCT+GGG | 0.539371 | 2_4:-18078768 | None:intergenic |
| AACTGGCGTGTCCTTTGTTC+GGG | 0.414172 | 2_4:+18087658 | Msa0318910:CDS |
| AAGAATCAGAAGGTGCAAAA+TGG | 0.304580 | 2_4:+18087630 | Msa0318910:CDS |
| AAGATTTAAGATAACTACAA+AGG | 0.601050 | 2_4:+18068726 | Msa0318910:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTACCCAAAATGTTACAATA+TGG | + | chr2_4:18067988-18068007 | Msa0318910:CDS | 30.0% | |
| ATCTCAAAATTCGCATTGAA+GGG | + | chr2_4:18068104-18068123 | Msa0318910:intron | 30.0% | |
| ATTGTCAAAGAGAACAACAA+TGG | - | chr2_4:18068524-18068543 | None:intergenic | 30.0% | |
| CATCTAGTGTTTCAATTGTT+AGG | + | chr2_4:18068665-18068684 | Msa0318910:CDS | 30.0% | |
| ATGAGCAAAACAATCATCTT+TGG | - | chr2_4:18068800-18068819 | None:intergenic | 30.0% | |
| TGGAACAAAAATGCCAATAA+TGG | + | chr2_4:18068915-18068934 | Msa0318910:CDS | 30.0% | |
| GGAACAAAAATGCCAATAAT+GGG | + | chr2_4:18068916-18068935 | Msa0318910:CDS | 30.0% | |
| TGTGTATAACTTCCAAATTG+AGG | + | chr2_4:18069568-18069587 | Msa0318910:intron | 30.0% | |
| AGTTTAAAGAGAGAGAAACT+TGG | + | chr2_4:18069666-18069685 | Msa0318910:intron | 30.0% | |
| GTTTAAAGAGAGAGAAACTT+GGG | + | chr2_4:18069667-18069686 | Msa0318910:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 18067095 | 18088102 | 18067095 | ID=Msa0318910;Name=Msa0318910 |
| chr2_4 | mRNA | 18067095 | 18088102 | 18067095 | ID=Msa0318910-mRNA-1;Parent=Msa0318910;Name=Msa0318910-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|0.25|0.07|13|0|1254 |
| chr2_4 | exon | 18067095 | 18067285 | 18067095 | ID=Msa0318910-mRNA-1:exon:6711;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18067577 | 18068079 | 18067577 | ID=Msa0318910-mRNA-1:exon:6712;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18068335 | 18068445 | 18068335 | ID=Msa0318910-mRNA-1:exon:6713;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18068476 | 18068717 | 18068476 | ID=Msa0318910-mRNA-1:exon:6714;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18068736 | 18068830 | 18068736 | ID=Msa0318910-mRNA-1:exon:6715;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18068852 | 18069072 | 18068852 | ID=Msa0318910-mRNA-1:exon:6716;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18077763 | 18077930 | 18077763 | ID=Msa0318910-mRNA-1:exon:6717;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18078128 | 18078175 | 18078128 | ID=Msa0318910-mRNA-1:exon:6718;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18078269 | 18078463 | 18078269 | ID=Msa0318910-mRNA-1:exon:6719;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18078518 | 18078583 | 18078518 | ID=Msa0318910-mRNA-1:exon:6720;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18078623 | 18078865 | 18078623 | ID=Msa0318910-mRNA-1:exon:6721;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18086150 | 18087055 | 18086150 | ID=Msa0318910-mRNA-1:exon:6722;Parent=Msa0318910-mRNA-1 |
| chr2_4 | exon | 18087327 | 18088102 | 18087327 | ID=Msa0318910-mRNA-1:exon:6723;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18067095 | 18067285 | 18067095 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18067577 | 18068079 | 18067577 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18068335 | 18068445 | 18068335 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18068476 | 18068717 | 18068476 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18068736 | 18068830 | 18068736 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18068852 | 18069072 | 18068852 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18077763 | 18077930 | 18077763 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18078128 | 18078175 | 18078128 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18078269 | 18078463 | 18078269 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18078518 | 18078583 | 18078518 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18078623 | 18078865 | 18078623 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18086150 | 18087055 | 18086150 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| chr2_4 | CDS | 18087327 | 18088102 | 18087327 | ID=Msa0318910-mRNA-1:cds;Parent=Msa0318910-mRNA-1 |
| Gene Sequence |
>Msa0318910 ATGGCTCAAATAATGTTTCAAGTTTTGGTGTCAATTCTCCTTGTGCTAGTGGCAGTGGAAGCAGCTACTCCTCCTGGAATTGCTAAAGATCATGAATATGGCCATGCAAGCTGCAAGATAAAGAAATACAAACATTGCTATAATTTAGTGCATGTATGTCCTAAGTTTTGCCCTAATGATTGTACTGTGGACCCTTCTACACCGAAAAACCCACCACCCTCATCGTCCACACCATCTTCTTCATCTAATTCGTCTTCCTCTTCATCATCTTCTTCTTCATCATCTTCATCTTCATCTTCCTCTTCTTCTTCATCATCATCATCACCTGAAAACCATTCAACACCACAAAGCTCACCACCTTCATCCTCTACACCATCTTCTTCTTCCTCTAATTCATCTTCTTCTGCTTCTTCTTCCTCTTCCGCAACTTCCTCTTCCTCTTCATCTTCATCACCTAACAGCCCTTCTACACCACATAACCCACCACCGTCTTCATCATCTACTCCTTCTTCATCTTCCTCTTCCTCTTCTTCTTCTTCTTCCTCCTCCTCCTCATCTTCTTCTTCAGCCTCACACAAAACTGCTAGATGTTGGAACAAGAACTACCCAAAATGTTACAATATGGAACATGTCTGTCCTAATGCATGCCGAGGCGGATGTGAGGTTGACTGCAACACTTGCAAACCAATTTGTAAGTGTGACAAACCAGGAGCTGTTTGCCAAGACCCTCGTTTCATTGGTGGTGATGGAATCACTTTTTACTTCCATGGCAAGAAAGACAACAACTTCTGCCTTGTTTCTGACAGTAGAAGAAACGAGAACATGAAAAGAGACTTCACATGGGTCCAATCCATTGTTGTTCTCTTTGACAATCACCAACTCTTCCTTGGAGCTCAAAAAACAGCAACATGGGATGACTCTGAAGACCGCCTTGCCCTCTCCTTTGATGGAGAACCCATCACCCTCCACGAATCAGAAGGTGCAAAATGGGAATCATCTAGTGTTTCAATTGTTAGGGAAACTTCCACAAACGACATTATTGTCGAAATAACTACAAAGGTTGTCCCTATAACCGAAGAAGACTCGAGGATTCACAATTATGGCATCACCAAAGATGATTGTTTTGCTCATCTTGATTTAGGCAATGAAGTGAGTGGTGTGTTGGGCCAAACATATAAACCTAACTATGTGAGTCGTGTGAACATTGGAACAAAAATGCCAATAATGGGAGGTGGAAAAGAGTATGAAACTACGAGCTTGTTCTCCGCAGATTGTTCCGTAGCTCGTTTTGTAGGTAATGACGGATCCAACAATGATATTGCGATGGTGGAGAATTTGAGTCTCCCTAGCATGAGTTGCACAATGGCAGTGGAAGCAGCTACTCCTCCTGGAATTGGTAAAGGTCGTGAGCAGGGTTATGCAACCTGCAAGATAAAGAAGTACAAGCATTGCTATAATTCGGTTCATGCATGTCCTAAGTCTTGTCCTTATGATTGTACTGTCGAGTGTGCCTCATGCAAACCTATTTGCTCGTGTGACAAACCAGGAGCTGTTTGCCAAGACCCTCGTTTCATTGGTGGTAGAAGAAACGAGAACATGAAAAGAGACTTCACCTGGGTCCAATCCATTGTTATTCTCTTTGACAATCACCAACTCTTCCTTGGAGCCCAAAAAACATCAACATGGGATGATTCTGTAGACCGTCTTGCCCTCTCCTTTGACGGCGAACCAATCACCCTCAACGAATCAGAAGGTGCGAAATGGGAATCATCTAGAAATCTTAGGATAATTGCTAAGGTTGTCCCTATAACTGAAGAAGACTCGAGGATTCACAATTATGGGTTCAAATTCTATACTCTAAGCAATGAAGTGAGTGGTGTGTTGGGCCAAACATATAAACCTAACTATGTGAGTCGTGTGAATATTGGAGCAAAAATGCCAATAATGGGAGGTGGAAAAGAGTATGAAACGTCAAGCTTGTTCTCCCCAGATTGTTCTGTAGCACGTTTCATTGGAAACAATGGATTAAACAAAGATATTGGAACTGTGAATAACTTGGCTCTCCCTAGCATGAGCTGCACAATGGCAGTGGAAGCAGCTACTACTCCTCCTGGAATTGCTAAAGATCATGAGTATGGTTATGCAACCTGCAAGATAAAGAAGTACAAACATTGCTATAATTTGGTGCATGTATGCCCTAAGTTTTGCCCTAATGATTGTACTGTGGAATGTGCCTCTTGCAAACCTATTTGTGTTGGGGGCACAAATCCTTCACATGATTATCCTATCCCTTCTACACCCCATAGCTCACCTTCATCATCATCTTCTTCATCCTCATCCTCTTCTTCTTCATCCGCTTCTTCTTCATCACCTAATAGCTCTCATACACCACAAAACCAACACAATGCATCTTCTACACCATCTTCATCCTCTTCATCATCATCGTCGTCGTCTTCTTCTTCTTCCTCCTCTTCATCAAGCCCTTCTACACCAAAAAACCCACCACCTTCACCATCTACACCATCTTCATCTAATTCGTCTTCCTCTTCATCCTCTTCTTCATCTTCATCTTCATCTTCTTCTTCCGCTTCTTCTTCTTCTTCATCGTCGTCATCACCTGAAAGCCATTCAACACCACAAAGCTCACCACCTTCATCGTCTACACCATCTTCTTCTTCATCTAATTCATCATCCTCTTCATCTTCTTCTGCTTCTTCTTCATCTTCCTCTTCCGCTACATCCTCTTCCTCTTCATCGCCTAACAACCCTTCTGCACCACATAACCCACCACCGTCTTCATCATCTACACCTTCATCTTCCTCTTCCTCTTCTTCTTCTTCTTCCTCTTCAGCTTCTTCTTCAGCCTCACACAAAACTGCTAGATGTCAGAACAAGAACTATCCAAAATGTTACAACATGGAACATTTATGTCCTAATGCATGCCCAGGTGGATGTGAGGTTGACTGCAACACTTGTAAACCAATTTGCAAGTGTGACAAACCAGGAGCTGTTTGCCAAGACCCACGTTTCATTGGTGGTGATGGAATCACTTTTTACTTCCACGGCAAGAAAGACAACAACTTCTGCCTTGTATCTGATTCCAACCTCCACATAAATGCTCACTTCATTGGTAGAAGAAACGAGAACATGAAGAGAGATTTCACTTGGGTGCAATCCATTGTTATTCTCTTTGACAATCACCAACTTTTCCTTGGAGCCCAAAAAACAGCAACATGGGATGACTCTGTGGATCGTCTTGCCCTCTCCTTTGACGGCGAACCCATCACCCTCCAAGAATCAGAAGGTGCAAAATGGGAATCAACTGGCGTGTCCTTTGTTCGGGAAACTTCCACAAACAATATTGTTGTTGAAGTTGAAGGAAGATTTAAGATAACTGCTAAGGTTGTCCCGATCACCGAAGAAGACTCGAAGATTCACAATTATGGCATCACCAAAGATGATTGTTTTGCTCACCTTGATTTAGGCTTCAAATTCTCTTCTCTAAGCAATGAAGTAAGTGGCGTATTGGGCCAAACATATAAACCGAACTATGTGAGTCGTGTGAACATTGGAGCGAAAATGCCAATAATGGGAGGTGGAAAAGAGTATGAAACAACAAGTTTGTTCTCCGCTGACTGTTCTGTAACTCGGTTTGTTGGTAACGTTGGATCCAACAATGACATTGCCATGGTGGATGATCTTATGCTCCCTAGCATGAGTTGCACAAGTGGTGTTGATGGCGAAGGAGTTGTTTGCAGGAGATAA |
| Protein sequence |
>Msa0318910 MAQIMFQVLVSILLVLVAVEAATPPGIAKDHEYGHASCKIKKYKHCYNLVHVCPKFCPNDCTVDPSTPKNPPPSSSTPSSSSNSSSSSSSSSSSSSSSSSSSSSSSSSPENHSTPQSSPPSSSTPSSSSSNSSSSASSSSSATSSSSSSSSPNSPSTPHNPPPSSSSTPSSSSSSSSSSSSSSSSSSSSASHKTARCWNKNYPKCYNMEHVCPNACRGGCEVDCNTCKPICKCDKPGAVCQDPRFIGGDGITFYFHGKKDNNFCLVSDSRRNENMKRDFTWVQSIVVLFDNHQLFLGAQKTATWDDSEDRLALSFDGEPITLHESEGAKWESSSVSIVRETSTNDIIVEITTKVVPITEEDSRIHNYGITKDDCFAHLDLGNEVSGVLGQTYKPNYVSRVNIGTKMPIMGGGKEYETTSLFSADCSVARFVGNDGSNNDIAMVENLSLPSMSCTMAVEAATPPGIGKGREQGYATCKIKKYKHCYNSVHACPKSCPYDCTVECASCKPICSCDKPGAVCQDPRFIGGRRNENMKRDFTWVQSIVILFDNHQLFLGAQKTSTWDDSVDRLALSFDGEPITLNESEGAKWESSRNLRIIAKVVPITEEDSRIHNYGFKFYTLSNEVSGVLGQTYKPNYVSRVNIGAKMPIMGGGKEYETSSLFSPDCSVARFIGNNGLNKDIGTVNNLALPSMSCTMAVEAATTPPGIAKDHEYGYATCKIKKYKHCYNLVHVCPKFCPNDCTVECASCKPICVGGTNPSHDYPIPSTPHSSPSSSSSSSSSSSSSSASSSSPNSSHTPQNQHNASSTPSSSSSSSSSSSSSSSSSSSSPSTPKNPPPSPSTPSSSNSSSSSSSSSSSSSSSSSASSSSSSSSSPESHSTPQSSPPSSSTPSSSSSNSSSSSSSSASSSSSSSATSSSSSSPNNPSAPHNPPPSSSSTPSSSSSSSSSSSSSASSSASHKTARCQNKNYPKCYNMEHLCPNACPGGCEVDCNTCKPICKCDKPGAVCQDPRFIGGDGITFYFHGKKDNNFCLVSDSNLHINAHFIGRRNENMKRDFTWVQSIVILFDNHQLFLGAQKTATWDDSVDRLALSFDGEPITLQESEGAKWESTGVSFVRETSTNNIVVEVEGRFKITAKVVPITEEDSKIHNYGITKDDCFAHLDLGFKFSSLSNEVSGVLGQTYKPNYVSRVNIGAKMPIMGGGKEYETTSLFSADCSVTRFVGNVGSNNDIAMVDDLMLPSMSCTSGVDGEGVVCRR* |
