AlfalfaGEDB | Msa0319990

M. sativa cultivar zhongmu-4 / Msa0319990

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009963 GO:0009987 GO:0010033 GO:0010224 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0042221 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0140110 GO:1900384 GO:1900386 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319990	XP_003594801.1	96.386	332	12	0	1	332	1	332	0.0	667

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319990	sp\|Q9FJ07\|MY111_ARATH	42.458	358	155	11	1	326	1	339	5.29e-72	229

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0319990	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0242540	Msa0319990	0.802191	6.307302e-49	-8.615850e-47
Msa0280750	Msa0319990	0.812968	3.246471e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319990	MtrunA17_Chr2g0294821	95.492	244	11	0	89	332	5	248	1.68e-174	483
Msa0319990	MtrunA17_Chr4g0068061	60.163	246	66	5	1	236	1	224	2.83e-92	278
Msa0319990	MtrunA17_Chr1g0194951	42.229	341	147	10	1	311	1	321	4.89e-69	220
Msa0319990	MtrunA17_Chr7g0269831	84.874	119	18	0	1	119	1	119	3.98e-65	209
Msa0319990	MtrunA17_Chr7g0275911	68.103	116	37	0	1	116	1	116	1.09e-59	192
Msa0319990	MtrunA17_Chr2g0294811	94.565	92	5	0	1	92	1	92	3.14e-59	186
Msa0319990	MtrunA17_Chr8g0346561	69.828	116	35	0	1	116	1	116	6.35e-59	191
Msa0319990	MtrunA17_Chr1g0167661	54.190	179	69	4	1	179	1	166	6.50e-59	190
Msa0319990	MtrunA17_Chr4g0070611	71.552	116	33	0	1	116	1	116	6.73e-59	192
Msa0319990	MtrunA17_Chr4g0070641	71.552	116	33	0	1	116	1	116	1.67e-58	186

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0319990	AT5G49330.1	42.458	358	155	11	1	326	1	339	5.38e-73	229
Msa0319990	AT3G62610.1	43.131	313	145	7	1	308	1	285	8.58e-70	221
Msa0319990	AT2G47460.1	61.988	171	39	2	1	169	1	147	7.35e-69	219
Msa0319990	AT1G06180.1	69.231	117	36	0	1	117	1	117	6.83e-59	190
Msa0319990	AT4G09460.1	65.152	132	40	1	1	126	1	132	5.13e-58	187
Msa0319990	AT3G61250.1	67.241	116	38	0	1	116	1	116	6.99e-58	189
Msa0319990	AT4G38620.1	58.025	162	56	2	1	150	1	162	8.02e-58	188
Msa0319990	AT1G35515.1	70.690	116	34	0	1	116	1	116	8.30e-58	186
Msa0319990	AT2G31180.1	49.730	185	86	3	1	185	1	178	1.98e-57	186
Msa0319990	AT1G22640.1	48.619	181	83	2	1	181	1	171	6.40e-57	185

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Find 71 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGTGAAGGATCTTGG+AGG	0.644525	2_4:+20115894	Msa0319990:CDS
AAAGATGCATGTGGAGATAA+TGG	0.348455	2_4:+20119734	Msa0319990:CDS
AAATGAATTGAATATTGATG+AGG	0.544069	2_4:+20119578	Msa0319990:CDS
AACAAAATGGTGAAGGATCT+TGG	0.342359	2_4:+20115891	Msa0319990:CDS
AAGCCATTTAGTATAGCCAA+TGG	0.571497	2_4:+20115784	Msa0319990:exon
AAGGCTTGCTTGTGGGGTCC+AGG	0.474360	2_4:+20119903	Msa0319990:CDS
AATAATATTTATGAAATGAA+GGG	0.558223	2_4:+20119519	Msa0319990:CDS
AATGAATTGAATATTGATGA+GGG	0.462618	2_4:+20119579	Msa0319990:CDS
AATGATTTCTTCCTCATGAG+GGG	0.620652	2_4:-20116158	None:intergenic
AATGCATGAGAATGATGATA+AGG	0.433723	2_4:+20119689	Msa0319990:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACATCAAACAAAATGGTGA+AGG	+	chr2_4:20115884-20115903	Msa0319990:CDS	30.0%
ATATCGAATTGAGAGTAAAG+CGG	+	chr2_4:20116419-20116438	Msa0319990:intron	30.0%
CATATTAAGTGTAGAGAAGT+GGG	-	chr2_4:20116789-20116808	None:intergenic	30.0%
ACATATTAAGTGTAGAGAAG+TGG	-	chr2_4:20116790-20116809	None:intergenic	30.0%
AATGTGAGAACGTTTATTCT+TGG	-	chr2_4:20117131-20117150	None:intergenic	30.0%
AAACATTTATGATTGACCTC+CGG	-	chr2_4:20117271-20117290	None:intergenic	30.0%
AAAATCAGTGTCCAAAGATA+TGG	-	chr2_4:20117309-20117328	None:intergenic	30.0%
ACTCTTCCTATGAAAATTGA+CGG	+	chr2_4:20117484-20117503	Msa0319990:intron	30.0%
ATGTTTCACAGAAACAAAGT+AGG	-	chr2_4:20117527-20117546	None:intergenic	30.0%
TGATACAATCAATACCTGAT+AGG	+	chr2_4:20117935-20117954	Msa0319990:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	20115777	20119959	20115777	ID=Msa0319990;Name=Msa0319990
chr2_4	mRNA	20115777	20119959	20115777	ID=Msa0319990-mRNA-1;Parent=Msa0319990;Name=Msa0319990-mRNA-1;_AED=0.08;_eAED=0.08;_QI=26\|1\|0.66\|1\|1\|1\|3\|0\|332
chr2_4	exon	20115777	20115935	20115777	ID=Msa0319990-mRNA-1:exon:7428;Parent=Msa0319990-mRNA-1
chr2_4	exon	20116080	20116209	20116080	ID=Msa0319990-mRNA-1:exon:7429;Parent=Msa0319990-mRNA-1
chr2_4	exon	20119224	20119959	20119224	ID=Msa0319990-mRNA-1:exon:7430;Parent=Msa0319990-mRNA-1
chr2_4	five_prime_UTR	20115777	20115802	20115777	ID=Msa0319990-mRNA-1:five_prime_utr;Parent=Msa0319990-mRNA-1
chr2_4	CDS	20115803	20115935	20115803	ID=Msa0319990-mRNA-1:cds;Parent=Msa0319990-mRNA-1
chr2_4	CDS	20116080	20116209	20116080	ID=Msa0319990-mRNA-1:cds;Parent=Msa0319990-mRNA-1
chr2_4	CDS	20119224	20119959	20119224	ID=Msa0319990-mRNA-1:cds;Parent=Msa0319990-mRNA-1

Gene Sequence

>Msa0319990

ATGGGAAGAGCTCCTTGTTGTGAAAAAGTTGGGTTGAAGAAAGGAAGATGGACTGCTGAAGAGGATAAAATCTTGACTGATTACATCAAACAAAATGGTGAAGGATCTTGGAGGTTTTTGCCTAAGAAAGCAGGTTTGTTAAGGTGTGGGAAGAGTTGTAGACTAAGATGGATCAACTATTTGAGAGCAGATGTGAAGAGAGGAAACATATCCCCTCATGAGGAAGAAATCATTGTCAAGTTGCATGCTGTTCTTGGAAATAGGTGGTCAGTGATAGCATCACATTTACCAGGACGAACAGATAATGAGATAAAGAACTATTGGAACTCTCATTTGAGAAGGAAAATTTACTGCTTCATGAGATCCATAAACGAGTCTCCACCACCCCTTGATATCGCATCTACATCCAAAACAATTAATCAAGGGAAAATAAATAATCCAAGCATAGAAGAAGACAATAGCATGGCTTTGATTCAAAATTCCCTTGAACCAATACCTAAAGAAAAAACAACTTCACAATACTATAATGTACAAGGAGTTGAAGATATTTCGTGTGAGAATAATATTTATGAAATGAAGGGTAACAAAAATAATACAGTTGCATCATACCCTAGTAAAAATGAATTGAATATTGATGAGGGTTTGGGACCATATAAATGGCTAGATGATGAAATTATGAAACTTCGTAACATGTTTTTAAATGGGATAGGAAATTATAATTATGTAACAATGCATGAGAATGATGATAAGGGTAATTATGAATGTGACTCTAGAAAGATGCATGTGGAGATAATGGAAACAAATAAGGAAATGAAAAGTGAAATTTGTGGCTCATCATATAATGGAGAGAGTGGAGAAGGGTACATTTCTAATGCATCAGTAAATTCTGCCACAGATTATGATCAATTGTGTGATTGGGACTTTTGTCAAGAAGACCAAATGAAGGCTTGCTTGTGGGGTCCAGGCATTGGTGAAATCATGAATGGTTTTTATCAGTAA

Protein sequence

>Msa0319990

MGRAPCCEKVGLKKGRWTAEEDKILTDYIKQNGEGSWRFLPKKAGLLRCGKSCRLRWINYLRADVKRGNISPHEEEIIVKLHAVLGNRWSVIASHLPGRTDNEIKNYWNSHLRRKIYCFMRSINESPPPLDIASTSKTINQGKINNPSIEEDNSMALIQNSLEPIPKEKTTSQYYNVQGVEDISCENNIYEMKGNKNNTVASYPSKNELNIDEGLGPYKWLDDEIMKLRNMFLNGIGNYNYVTMHENDDKGNYECDSRKMHVEIMETNKEMKSEICGSSYNGESGEGYISNASVNSATDYDQLCDWDFCQEDQMKACLWGPGIGEIMNGFYQ*