Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0324710 | XP_003595397.1 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 4.38e-49 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0308750 | Msa0324710 | 0.819720 | 1.001691e-52 | -8.615850e-47 |
| Msa0316740 | Msa0324710 | 0.818458 | 1.939375e-52 | -8.615850e-47 |
| Msa0324710 | Msa0844360 | 0.808253 | 3.390792e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0324710 | MtrunA17_Chr2g0302081 | 100.000 | 78 | 0 | 0 | 1 | 78 | 1 | 78 | 4.03e-53 | 159 |
| Msa0324710 | MtrunA17_Chr2g0302071 | 70.513 | 78 | 23 | 0 | 1 | 78 | 1 | 78 | 8.33e-35 | 113 |
| Msa0324710 | MtrunA17_Chr4g0069511 | 59.756 | 82 | 27 | 2 | 1 | 78 | 1 | 80 | 1.30e-25 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0324710 | AT3G05858.1 | 52.500 | 80 | 33 | 3 | 1 | 75 | 1 | 80 | 6.62e-16 | 66.2 |
| Msa0324710 | AT5G26620.1 | 45.333 | 75 | 30 | 1 | 1 | 75 | 1 | 64 | 1.05e-14 | 62.8 |
Find 14 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGAAACAATGATGAATAT+TGG | 0.265744 | 2_4:-30563601 | Msa0324710:CDS |
| AATTAATTCAAGAAGCAAAT+TGG | 0.309379 | 2_4:+30563441 | None:intergenic |
| TGGAAACAATGATGAATATT+GGG | 0.371421 | 2_4:-30563600 | Msa0324710:CDS |
| TATTGGGAAGGTAGTGGAAG+TGG | 0.432591 | 2_4:-30563584 | Msa0324710:CDS |
| AGCTTTGAATACATTGCATA+AGG | 0.453917 | 2_4:+30563634 | None:intergenic |
| GGTTGTTGGGTTGCTGAGCC+TGG | 0.459748 | 2_4:-30563530 | Msa0324710:CDS |
| TTCAAAGCTTGTTTCTCAAG+TGG | 0.476215 | 2_4:-30563620 | Msa0324710:CDS |
| TGATGAAGATAGAGGTTGTT+GGG | 0.482176 | 2_4:-30563543 | Msa0324710:CDS |
| GATGAATATTGGGAAGGTAG+TGG | 0.519319 | 2_4:-30563590 | Msa0324710:CDS |
| GTGATGAAGATAGAGGTTGT+TGG | 0.556565 | 2_4:-30563544 | Msa0324710:CDS |
| AACAATGATGAATATTGGGA+AGG | 0.569030 | 2_4:-30563596 | Msa0324710:CDS |
| TTGCTGAGCCTGGTATTGAT+AGG | 0.577391 | 2_4:-30563520 | Msa0324710:CDS |
| TTTGCAAGTGATGAAGATAG+AGG | 0.587569 | 2_4:-30563551 | Msa0324710:CDS |
| GAAGCTTTCCTATCAATACC+AGG | 0.623221 | 2_4:+30563512 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGGAAACAATGATGAATATT+GGG | - | chr2_4:30563510-30563529 | Msa0324710:CDS | 25.0% |
| !! | AGCTTTGAATACATTGCATA+AGG | + | chr2_4:30563479-30563498 | None:intergenic | 30.0% |
| GTGGAAACAATGATGAATAT+TGG | - | chr2_4:30563509-30563528 | Msa0324710:CDS | 30.0% | |
| AACAATGATGAATATTGGGA+AGG | - | chr2_4:30563514-30563533 | Msa0324710:CDS | 30.0% | |
| ! | TTCAAAGCTTGTTTCTCAAG+TGG | - | chr2_4:30563490-30563509 | Msa0324710:CDS | 35.0% |
| TTTGCAAGTGATGAAGATAG+AGG | - | chr2_4:30563559-30563578 | Msa0324710:CDS | 35.0% | |
| TGATGAAGATAGAGGTTGTT+GGG | - | chr2_4:30563567-30563586 | Msa0324710:CDS | 35.0% | |
| GATGAATATTGGGAAGGTAG+TGG | - | chr2_4:30563520-30563539 | Msa0324710:CDS | 40.0% | |
| GTGATGAAGATAGAGGTTGT+TGG | - | chr2_4:30563566-30563585 | Msa0324710:CDS | 40.0% | |
| GAAGCTTTCCTATCAATACC+AGG | + | chr2_4:30563601-30563620 | None:intergenic | 40.0% | |
| !! | TATTGGGAAGGTAGTGGAAG+TGG | - | chr2_4:30563526-30563545 | Msa0324710:CDS | 45.0% |
| !! | TTGCTGAGCCTGGTATTGAT+AGG | - | chr2_4:30563590-30563609 | Msa0324710:CDS | 45.0% |
| ! | GGTTGTTGGGTTGCTGAGCC+TGG | - | chr2_4:30563580-30563599 | Msa0324710:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 30563448 | 30563684 | 30563448 | ID=Msa0324710;Name=Msa0324710 |
| chr2_4 | mRNA | 30563448 | 30563684 | 30563448 | ID=Msa0324710-mRNA-1;Parent=Msa0324710;Name=Msa0324710-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|78 |
| chr2_4 | exon | 30563448 | 30563684 | 30563448 | ID=Msa0324710-mRNA-1:exon:10245;Parent=Msa0324710-mRNA-1 |
| chr2_4 | CDS | 30563448 | 30563684 | 30563448 | ID=Msa0324710-mRNA-1:cds;Parent=Msa0324710-mRNA-1 |
| Gene Sequence |
| Protein sequence |
