AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0327160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTGGTTCAAATTGTATT+TGG 0.160383 2_4:-38679664 Msa0327160:five_prime_UTR
AGTTACTAGTTTATGAAAAT+TGG 0.199311 2_4:-38679606 Msa0327160:five_prime_UTR
TTTATGAAAATTGGCATTAC+AGG 0.230305 2_4:-38679597 Msa0327160:five_prime_UTR
CGATAAATCTTATTCGAAAA+TGG 0.247519 2_4:-38679027 Msa0327160:three_prime_UTR
CTGTGTCACTCAGGGCTTCA+TGG 0.269848 2_4:-38679832 Msa0327160:five_prime_UTR
TCACTCTCTTATTGCATTGC+TGG 0.285106 2_4:+38679631 None:intergenic
GCTAAATGCTTACCTGAAAA+TGG 0.304694 2_4:-38679455 Msa0327160:five_prime_UTR
GAGCTGGCGTTTGTTGATTC+AGG 0.310318 2_4:+38680245 None:intergenic
GGACTAACACCTCTGATAAA+AGG 0.315262 2_4:-38679947 Msa0327160:five_prime_UTR
TTTATCGCTGTTGTGGTTGC+AGG 0.349400 2_4:+38679043 None:intergenic
TTCATATATACCTTATTGTT+CGG 0.386389 2_4:-38680743 Msa0327160:five_prime_UTR
TTTCAGTTGGGTGGACCAAA+TGG 0.392422 2_4:-38678916 Msa0327160:intron
ACTTGGATGTCTTCAAATCA+TGG 0.408351 2_4:-38679773 Msa0327160:five_prime_UTR
AGGAACAGAACCGAACAATA+AGG 0.421071 2_4:+38680733 None:intergenic
GCTTAATTTAACTTGGAAGT+AGG 0.432193 2_4:-38679537 Msa0327160:five_prime_UTR
CTTAATTTAACTTGGAAGTA+GGG 0.437526 2_4:-38679536 Msa0327160:five_prime_UTR
ATGCTTACCTGAAAATGGTT+AGG 0.446438 2_4:-38679450 Msa0327160:five_prime_UTR
ATGTTTGAGAACAAATGAAA+AGG 0.458265 2_4:-38680212 Msa0327160:five_prime_UTR
GTCCGGTGTCTGTGTCACTC+AGG 0.458818 2_4:-38679841 Msa0327160:five_prime_UTR
CACAGAACCACTCAGTTTGT+AGG 0.459294 2_4:-38680843 Msa0327160:five_prime_UTR
ATATAATCACATGTGTCATC+TGG 0.460556 2_4:+38679910 None:intergenic
GTGGCAAGGCTTGTTGAGCT+TGG 0.460871 2_4:-38679363 Msa0327160:CDS
AAATGGTTAGGAATTGAAAC+TGG 0.465677 2_4:-38679438 Msa0327160:five_prime_UTR
TATGTTGGCTTAATTTAACT+TGG 0.474725 2_4:-38679544 Msa0327160:five_prime_UTR
CGTACGGCAATTTAAAACCT+TGG 0.478720 2_4:-38679680 Msa0327160:five_prime_UTR
TCATCAAATTACAAACAGTC+AGG 0.484611 2_4:+38680917 None:intergenic
GGCTTCATGGATGTTTATGA+TGG 0.499181 2_4:-38679819 Msa0327160:five_prime_UTR
TCCGGTGTCTGTGTCACTCA+GGG 0.500314 2_4:-38679840 Msa0327160:five_prime_UTR
AAACATCAAACAAGCTGAGC+TGG 0.500801 2_4:+38680229 None:intergenic
TGTTTGAGAACAAATGAAAA+GGG 0.508018 2_4:-38680211 Msa0327160:five_prime_UTR
GCTTAAATTGCAGCTGCGTA+CGG 0.508203 2_4:-38679696 Msa0327160:five_prime_UTR
TAAGAAATTTGTATGTATGT+TGG 0.510169 2_4:-38679559 Msa0327160:five_prime_UTR
ACAGAACCACTCAGTTTGTA+GGG 0.512265 2_4:-38680842 Msa0327160:five_prime_UTR
AATAAGATTTATCGCTGTTG+TGG 0.514886 2_4:+38679036 None:intergenic
AAGGAGTTGATAGAATATAC+AGG 0.522584 2_4:+38680568 None:intergenic
ATTTCGATGCAACAGAAACC+TGG 0.523035 2_4:-38680280 Msa0327160:five_prime_UTR
ACAGCATGTGGTGGAGGTGG+CGG 0.525377 2_4:+38680774 None:intergenic
AAATCTTATTCGAAAATGGA+TGG 0.534286 2_4:-38679023 Msa0327160:three_prime_UTR
GCAACAGAAACCTGGAAAAG+AGG 0.537275 2_4:-38680272 Msa0327160:five_prime_UTR
TCCAAATACAATTTGAACCA+AGG 0.541762 2_4:+38679663 None:intergenic
TTAATTTAACTTGGAAGTAG+GGG 0.548072 2_4:-38679535 Msa0327160:five_prime_UTR
CATGCACGAGTGAGAAATGC+TGG 0.553352 2_4:+38680818 None:intergenic
TGTTGAATATTAGTTCGACT+TGG 0.559934 2_4:-38679790 Msa0327160:five_prime_UTR
CGAAAATGGATGGAAGATGC+TGG 0.561977 2_4:-38679013 Msa0327160:three_prime_UTR
AGAACAGCATGTGGTGGAGG+TGG 0.565480 2_4:+38680771 None:intergenic
CGATGTGTCATTATCGTGTC+CGG 0.569909 2_4:-38679858 Msa0327160:five_prime_UTR
TTGTTAGCTCTGTAGCACTA+AGG 0.577763 2_4:-38679968 Msa0327160:five_prime_UTR
GAAAGAACAGCATGTGGTGG+AGG 0.581921 2_4:+38680768 None:intergenic
AAAGAGAGTTAGCGAAAACT+CGG 0.583465 2_4:-38680700 Msa0327160:five_prime_UTR
GCCTCATCTCTTAGAATCTG+CGG 0.585167 2_4:+38679387 None:intergenic
TCACATGTGTCATCTGGTGT+TGG 0.599539 2_4:+38679916 None:intergenic
GAAAACAAAGAAGCACATGA+AGG 0.610863 2_4:+38680549 None:intergenic
TCCGCAGATTCTAAGAGATG+AGG 0.611127 2_4:-38679388 Msa0327160:exon
AAGGCTTGTTGAGCTTGGAA+AGG 0.612966 2_4:-38679358 Msa0327160:intron
GATTCTAAGAGATGAGGCAG+TGG 0.632621 2_4:-38679382 Msa0327160:exon
ATGCTAGTGGCTATCTAGAG+AGG 0.634407 2_4:-38679078 Msa0327160:exon
GCCCTGAGTGACACAGACAC+CGG 0.647988 2_4:+38679839 None:intergenic
TAAGAGATGAGGCAGTGGCA+AGG 0.651166 2_4:-38679377 Msa0327160:exon
CATGTGTCATCTGGTGTTGG+AGG 0.653553 2_4:+38679919 None:intergenic
CATCAAATTACAAACAGTCA+GGG 0.659874 2_4:+38680918 None:intergenic
AGTCAAAGCAAGAACAACAA+TGG 0.663394 2_4:+38680158 None:intergenic
GAACAGGTTAGTGATGCTAG+TGG 0.681751 2_4:-38679091 Msa0327160:intron
ATTGAAAGAACAGCATGTGG+TGG 0.691465 2_4:+38680765 None:intergenic
TGAATTGAAAGAACAGCATG+TGG 0.716001 2_4:+38680762 None:intergenic
GCTGGACCCTACAAACTGAG+TGG 0.730023 2_4:+38680836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGTTATTTTTGATTTA+TGG - chr2_4:38678918-38678937 Msa0327160:intron 10.0%
!!! ATATTGTTATTTTTGATTTA+TGG - chr2_4:38678918-38678937 Msa0327160:intron 10.0%
!! TAAAAATAACATCTGAATAA+AGG - chr2_4:38679383-38679402 Msa0327160:exon 15.0%
!! TAAAAATAACATCTGAATAA+AGG - chr2_4:38679383-38679402 Msa0327160:exon 15.0%
!! TTCATATATACCTTATTGTT+CGG - chr2_4:38679116-38679135 Msa0327160:intron 20.0%
!!! TTTGTTTTTGTTTTGATTCT+TGG - chr2_4:38679462-38679481 Msa0327160:five_prime_UTR 20.0%
!!! AGTTACTAGTTTATGAAAAT+TGG - chr2_4:38680253-38680272 Msa0327160:five_prime_UTR 20.0%
!! TAAGAAATTTGTATGTATGT+TGG - chr2_4:38680300-38680319 Msa0327160:intron 20.0%
!!! ATGAATATGTTATTTTCAGT+TGG - chr2_4:38680930-38680949 Msa0327160:five_prime_UTR 20.0%
!!! TGAATATGTTATTTTCAGTT+GGG - chr2_4:38680931-38680950 Msa0327160:five_prime_UTR 20.0%
!! TTCATATATACCTTATTGTT+CGG - chr2_4:38679116-38679135 Msa0327160:intron 20.0%
!!! TTTGTTTTTGTTTTGATTCT+TGG - chr2_4:38679462-38679481 Msa0327160:five_prime_UTR 20.0%
!!! AGTTACTAGTTTATGAAAAT+TGG - chr2_4:38680253-38680272 Msa0327160:five_prime_UTR 20.0%
!! TAAGAAATTTGTATGTATGT+TGG - chr2_4:38680300-38680319 Msa0327160:intron 20.0%
!!! ATGAATATGTTATTTTCAGT+TGG - chr2_4:38680930-38680949 Msa0327160:five_prime_UTR 20.0%
!!! TGAATATGTTATTTTCAGTT+GGG - chr2_4:38680931-38680950 Msa0327160:five_prime_UTR 20.0%
! ACAAAGAAACATGAAAGAAA+AGG + chr2_4:38679346-38679365 None:intergenic 25.0%
!! AATGTTAAGATACCCTTTTA+AGG - chr2_4:38679497-38679516 Msa0327160:five_prime_UTR 25.0%
!! TTTTGCTAACATCATAGAAA+AGG + chr2_4:38679532-38679551 None:intergenic 25.0%
! ATGTTTGAGAACAAATGAAA+AGG - chr2_4:38679647-38679666 Msa0327160:five_prime_UTR 25.0%
! TGTTTGAGAACAAATGAAAA+GGG - chr2_4:38679648-38679667 Msa0327160:five_prime_UTR 25.0%
! TTTATGAAAATTGGCATTAC+AGG - chr2_4:38680262-38680281 Msa0327160:five_prime_UTR 25.0%
!!! TATGTTGGCTTAATTTAACT+TGG - chr2_4:38680315-38680334 Msa0327160:intron 25.0%
!!! CTTAATTTAACTTGGAAGTA+GGG - chr2_4:38680323-38680342 Msa0327160:intron 25.0%
!!! TTAATTTAACTTGGAAGTAG+GGG - chr2_4:38680324-38680343 Msa0327160:intron 25.0%
! AAACTGGAGATTTAGAAAAA+CGG - chr2_4:38680437-38680456 Msa0327160:intron 25.0%
! CATTGAGTAAATATCCAAAT+TGG - chr2_4:38680584-38680603 Msa0327160:five_prime_UTR 25.0%
! TACAATTTCAAGAACCAATT+TGG + chr2_4:38680601-38680620 None:intergenic 25.0%
! CGATAAATCTTATTCGAAAA+TGG - chr2_4:38680832-38680851 Msa0327160:five_prime_UTR 25.0%
! AAATCTTATTCGAAAATGGA+TGG - chr2_4:38680836-38680855 Msa0327160:five_prime_UTR 25.0%
! TTAGAGTTAAAGAAGTTAGA+AGG + chr2_4:38680886-38680905 None:intergenic 25.0%
! ACAAAGAAACATGAAAGAAA+AGG + chr2_4:38679346-38679365 None:intergenic 25.0%
!! AATGTTAAGATACCCTTTTA+AGG - chr2_4:38679497-38679516 Msa0327160:five_prime_UTR 25.0%
!! TTTTGCTAACATCATAGAAA+AGG + chr2_4:38679532-38679551 None:intergenic 25.0%
! ATGTTTGAGAACAAATGAAA+AGG - chr2_4:38679647-38679666 Msa0327160:five_prime_UTR 25.0%
! TGTTTGAGAACAAATGAAAA+GGG - chr2_4:38679648-38679667 Msa0327160:five_prime_UTR 25.0%
! TTTATGAAAATTGGCATTAC+AGG - chr2_4:38680262-38680281 Msa0327160:five_prime_UTR 25.0%
!!! TATGTTGGCTTAATTTAACT+TGG - chr2_4:38680315-38680334 Msa0327160:intron 25.0%
!!! CTTAATTTAACTTGGAAGTA+GGG - chr2_4:38680323-38680342 Msa0327160:intron 25.0%
!!! TTAATTTAACTTGGAAGTAG+GGG - chr2_4:38680324-38680343 Msa0327160:intron 25.0%
! AAACTGGAGATTTAGAAAAA+CGG - chr2_4:38680437-38680456 Msa0327160:intron 25.0%
! CATTGAGTAAATATCCAAAT+TGG - chr2_4:38680584-38680603 Msa0327160:five_prime_UTR 25.0%
! TACAATTTCAAGAACCAATT+TGG + chr2_4:38680601-38680620 None:intergenic 25.0%
! CGATAAATCTTATTCGAAAA+TGG - chr2_4:38680832-38680851 Msa0327160:five_prime_UTR 25.0%
! AAATCTTATTCGAAAATGGA+TGG - chr2_4:38680836-38680855 Msa0327160:five_prime_UTR 25.0%
! TTAGAGTTAAAGAAGTTAGA+AGG + chr2_4:38680886-38680905 None:intergenic 25.0%
CATCAAATTACAAACAGTCA+GGG + chr2_4:38678944-38678963 None:intergenic 30.0%
TCATCAAATTACAAACAGTC+AGG + chr2_4:38678945-38678964 None:intergenic 30.0%
! AACTCTCTTTTCAATCTCAA+AGG + chr2_4:38679149-38679168 None:intergenic 30.0%
!! AAGGAGTTGATAGAATATAC+AGG + chr2_4:38679294-38679313 None:intergenic 30.0%
TTGATTCTTGGAATCTTTGA+TGG - chr2_4:38679474-38679493 Msa0327160:five_prime_UTR 30.0%
AGGAATCTGAATCCTTAAAA+GGG + chr2_4:38679512-38679531 None:intergenic 30.0%
AAGGAATCTGAATCCTTAAA+AGG + chr2_4:38679513-38679532 None:intergenic 30.0%
ATATAATCACATGTGTCATC+TGG + chr2_4:38679952-38679971 None:intergenic 30.0%
TGTTGAATATTAGTTCGACT+TGG - chr2_4:38680069-38680088 Msa0327160:five_prime_UTR 30.0%
ACCTTGGTTCAAATTGTATT+TGG - chr2_4:38680195-38680214 Msa0327160:five_prime_UTR 30.0%
TCCAAATACAATTTGAACCA+AGG + chr2_4:38680199-38680218 None:intergenic 30.0%
!! GCTTAATTTAACTTGGAAGT+AGG - chr2_4:38680322-38680341 Msa0327160:intron 30.0%
! TTCAATTCCTAACCATTTTC+AGG + chr2_4:38680419-38680438 None:intergenic 30.0%
AAATGGTTAGGAATTGAAAC+TGG - chr2_4:38680421-38680440 Msa0327160:intron 30.0%
AACTGGAGATTTAGAAAAAC+GGG - chr2_4:38680438-38680457 Msa0327160:intron 30.0%
AAAGATGAGTTCTTGAAGTT+AGG + chr2_4:38680529-38680548 None:intergenic 30.0%
!!! TGCAGTTTTAACTATGTTGA+CGG + chr2_4:38680566-38680585 None:intergenic 30.0%
AATAAGATTTATCGCTGTTG+TGG + chr2_4:38680826-38680845 None:intergenic 30.0%
!!! ATATGTTATTTTCAGTTGGG+TGG - chr2_4:38680934-38680953 Msa0327160:five_prime_UTR 30.0%
CATCAAATTACAAACAGTCA+GGG + chr2_4:38678944-38678963 None:intergenic 30.0%
TCATCAAATTACAAACAGTC+AGG + chr2_4:38678945-38678964 None:intergenic 30.0%
! AACTCTCTTTTCAATCTCAA+AGG + chr2_4:38679149-38679168 None:intergenic 30.0%
!! AAGGAGTTGATAGAATATAC+AGG + chr2_4:38679294-38679313 None:intergenic 30.0%
TTGATTCTTGGAATCTTTGA+TGG - chr2_4:38679474-38679493 Msa0327160:five_prime_UTR 30.0%
AGGAATCTGAATCCTTAAAA+GGG + chr2_4:38679512-38679531 None:intergenic 30.0%
AAGGAATCTGAATCCTTAAA+AGG + chr2_4:38679513-38679532 None:intergenic 30.0%
ATATAATCACATGTGTCATC+TGG + chr2_4:38679952-38679971 None:intergenic 30.0%
TGTTGAATATTAGTTCGACT+TGG - chr2_4:38680069-38680088 Msa0327160:five_prime_UTR 30.0%
ACCTTGGTTCAAATTGTATT+TGG - chr2_4:38680195-38680214 Msa0327160:five_prime_UTR 30.0%
TCCAAATACAATTTGAACCA+AGG + chr2_4:38680199-38680218 None:intergenic 30.0%
!! GCTTAATTTAACTTGGAAGT+AGG - chr2_4:38680322-38680341 Msa0327160:intron 30.0%
! TTCAATTCCTAACCATTTTC+AGG + chr2_4:38680419-38680438 None:intergenic 30.0%
AAATGGTTAGGAATTGAAAC+TGG - chr2_4:38680421-38680440 Msa0327160:intron 30.0%
AACTGGAGATTTAGAAAAAC+GGG - chr2_4:38680438-38680457 Msa0327160:intron 30.0%
AAAGATGAGTTCTTGAAGTT+AGG + chr2_4:38680529-38680548 None:intergenic 30.0%
!!! TGCAGTTTTAACTATGTTGA+CGG + chr2_4:38680566-38680585 None:intergenic 30.0%
AATAAGATTTATCGCTGTTG+TGG + chr2_4:38680826-38680845 None:intergenic 30.0%
!!! ATATGTTATTTTCAGTTGGG+TGG - chr2_4:38680934-38680953 Msa0327160:five_prime_UTR 30.0%
TGAATTGAAAGAACAGCATG+TGG + chr2_4:38679100-38679119 None:intergenic 35.0%
AAAGAGAGTTAGCGAAAACT+CGG - chr2_4:38679159-38679178 Msa0327160:intron 35.0%
! GAAAACAAAGAAGCACATGA+AGG + chr2_4:38679313-38679332 None:intergenic 35.0%
!!! TCATGTGCTTCTTTGTTTTC+TGG - chr2_4:38679313-38679332 Msa0327160:intron 35.0%
AGTCAAAGCAAGAACAACAA+TGG + chr2_4:38679704-38679723 None:intergenic 35.0%
! TCTTGCTTTGACTCTTTTTC+TGG - chr2_4:38679711-38679730 Msa0327160:five_prime_UTR 35.0%
!!! GTTGTGATGTGTGTTTTTTG+TGG - chr2_4:38679854-38679873 Msa0327160:five_prime_UTR 35.0%
ACTTGGATGTCTTCAAATCA+TGG - chr2_4:38680086-38680105 Msa0327160:five_prime_UTR 35.0%
! AATTGGCATTACAGGTTTTG+TGG - chr2_4:38680270-38680289 Msa0327160:five_prime_UTR 35.0%
GCTAAATGCTTACCTGAAAA+TGG - chr2_4:38680404-38680423 Msa0327160:intron 35.0%
ATGCTTACCTGAAAATGGTT+AGG - chr2_4:38680409-38680428 Msa0327160:intron 35.0%
ACTGGAGATTTAGAAAAACG+GGG - chr2_4:38680439-38680458 Msa0327160:intron 35.0%
GGTTCTTGAAATTGTAAGAC+TGG - chr2_4:38680605-38680624 Msa0327160:five_prime_UTR 35.0%
TATGCAACACTCTGCATAAA+AGG - chr2_4:38680669-38680688 Msa0327160:five_prime_UTR 35.0%
TTAGACGTTAATGCTGTTGT+TGG - chr2_4:38680743-38680762 Msa0327160:five_prime_UTR 35.0%
TGAATTGAAAGAACAGCATG+TGG + chr2_4:38679100-38679119 None:intergenic 35.0%
AAAGAGAGTTAGCGAAAACT+CGG - chr2_4:38679159-38679178 Msa0327160:intron 35.0%
! GAAAACAAAGAAGCACATGA+AGG + chr2_4:38679313-38679332 None:intergenic 35.0%
!!! TCATGTGCTTCTTTGTTTTC+TGG - chr2_4:38679313-38679332 Msa0327160:intron 35.0%
AGTCAAAGCAAGAACAACAA+TGG + chr2_4:38679704-38679723 None:intergenic 35.0%
! TCTTGCTTTGACTCTTTTTC+TGG - chr2_4:38679711-38679730 Msa0327160:five_prime_UTR 35.0%
!!! GTTGTGATGTGTGTTTTTTG+TGG - chr2_4:38679854-38679873 Msa0327160:five_prime_UTR 35.0%
ACTTGGATGTCTTCAAATCA+TGG - chr2_4:38680086-38680105 Msa0327160:five_prime_UTR 35.0%
! AATTGGCATTACAGGTTTTG+TGG - chr2_4:38680270-38680289 Msa0327160:five_prime_UTR 35.0%
GCTAAATGCTTACCTGAAAA+TGG - chr2_4:38680404-38680423 Msa0327160:intron 35.0%
ATGCTTACCTGAAAATGGTT+AGG - chr2_4:38680409-38680428 Msa0327160:intron 35.0%
ACTGGAGATTTAGAAAAACG+GGG - chr2_4:38680439-38680458 Msa0327160:intron 35.0%
GGTTCTTGAAATTGTAAGAC+TGG - chr2_4:38680605-38680624 Msa0327160:five_prime_UTR 35.0%
TATGCAACACTCTGCATAAA+AGG - chr2_4:38680669-38680688 Msa0327160:five_prime_UTR 35.0%
TTAGACGTTAATGCTGTTGT+TGG - chr2_4:38680743-38680762 Msa0327160:five_prime_UTR 35.0%
ACAGAACCACTCAGTTTGTA+GGG - chr2_4:38679017-38679036 Msa0327160:three_prime_UTR 40.0%
ATTGAAAGAACAGCATGTGG+TGG + chr2_4:38679097-38679116 None:intergenic 40.0%
AGGAACAGAACCGAACAATA+AGG + chr2_4:38679129-38679148 None:intergenic 40.0%
ATTTCGATGCAACAGAAACC+TGG - chr2_4:38679579-38679598 Msa0327160:five_prime_UTR 40.0%
AAACATCAAACAAGCTGAGC+TGG + chr2_4:38679633-38679652 None:intergenic 40.0%
!! TTGTTAGCTCTGTAGCACTA+AGG - chr2_4:38679891-38679910 Msa0327160:five_prime_UTR 40.0%
GGACTAACACCTCTGATAAA+AGG - chr2_4:38679912-38679931 Msa0327160:five_prime_UTR 40.0%
GGCTTCATGGATGTTTATGA+TGG - chr2_4:38680040-38680059 Msa0327160:five_prime_UTR 40.0%
! CGTACGGCAATTTAAAACCT+TGG - chr2_4:38680179-38680198 Msa0327160:five_prime_UTR 40.0%
TCACTCTCTTATTGCATTGC+TGG + chr2_4:38680231-38680250 None:intergenic 40.0%
AATGCTGTTGTTGGATGAAC+AGG - chr2_4:38680752-38680771 Msa0327160:five_prime_UTR 40.0%
ACAGAACCACTCAGTTTGTA+GGG - chr2_4:38679017-38679036 Msa0327160:three_prime_UTR 40.0%
ATTGAAAGAACAGCATGTGG+TGG + chr2_4:38679097-38679116 None:intergenic 40.0%
AGGAACAGAACCGAACAATA+AGG + chr2_4:38679129-38679148 None:intergenic 40.0%
ATTTCGATGCAACAGAAACC+TGG - chr2_4:38679579-38679598 Msa0327160:five_prime_UTR 40.0%
AAACATCAAACAAGCTGAGC+TGG + chr2_4:38679633-38679652 None:intergenic 40.0%
!! TTGTTAGCTCTGTAGCACTA+AGG - chr2_4:38679891-38679910 Msa0327160:five_prime_UTR 40.0%
GGACTAACACCTCTGATAAA+AGG - chr2_4:38679912-38679931 Msa0327160:five_prime_UTR 40.0%
GGCTTCATGGATGTTTATGA+TGG - chr2_4:38680040-38680059 Msa0327160:five_prime_UTR 40.0%
! CGTACGGCAATTTAAAACCT+TGG - chr2_4:38680179-38680198 Msa0327160:five_prime_UTR 40.0%
TCACTCTCTTATTGCATTGC+TGG + chr2_4:38680231-38680250 None:intergenic 40.0%
AATGCTGTTGTTGGATGAAC+AGG - chr2_4:38680752-38680771 Msa0327160:five_prime_UTR 40.0%
CACAGAACCACTCAGTTTGT+AGG - chr2_4:38679016-38679035 Msa0327160:three_prime_UTR 45.0%
ATAGTGTGTGTGTGTGTGTG+TGG + chr2_4:38679235-38679254 None:intergenic 45.0%
GCAACAGAAACCTGGAAAAG+AGG - chr2_4:38679587-38679606 Msa0327160:five_prime_UTR 45.0%
! GAGGACACACCTTTTATCAG+AGG + chr2_4:38679924-38679943 None:intergenic 45.0%
TCACATGTGTCATCTGGTGT+TGG + chr2_4:38679946-38679965 None:intergenic 45.0%
! CGATGTGTCATTATCGTGTC+CGG - chr2_4:38680001-38680020 Msa0327160:five_prime_UTR 45.0%
GCTTAAATTGCAGCTGCGTA+CGG - chr2_4:38680163-38680182 Msa0327160:five_prime_UTR 45.0%
! TCCGCAGATTCTAAGAGATG+AGG - chr2_4:38680471-38680490 Msa0327160:intron 45.0%
GCCTCATCTCTTAGAATCTG+CGG + chr2_4:38680475-38680494 None:intergenic 45.0%
! GATTCTAAGAGATGAGGCAG+TGG - chr2_4:38680477-38680496 Msa0327160:intron 45.0%
!! AAGGCTTGTTGAGCTTGGAA+AGG - chr2_4:38680501-38680520 Msa0327160:intron 45.0%
GAACAGGTTAGTGATGCTAG+TGG - chr2_4:38680768-38680787 Msa0327160:five_prime_UTR 45.0%
ATGCTAGTGGCTATCTAGAG+AGG - chr2_4:38680781-38680800 Msa0327160:five_prime_UTR 45.0%
TTTATCGCTGTTGTGGTTGC+AGG + chr2_4:38680819-38680838 None:intergenic 45.0%
CGAAAATGGATGGAAGATGC+TGG - chr2_4:38680846-38680865 Msa0327160:five_prime_UTR 45.0%
TTTCAGTTGGGTGGACCAAA+TGG - chr2_4:38680943-38680962 Msa0327160:five_prime_UTR 45.0%
TTCAGTTGGGTGGACCAAAT+GGG - chr2_4:38680944-38680963 Msa0327160:five_prime_UTR 45.0%
CACAGAACCACTCAGTTTGT+AGG - chr2_4:38679016-38679035 Msa0327160:three_prime_UTR 45.0%
ATAGTGTGTGTGTGTGTGTG+TGG + chr2_4:38679235-38679254 None:intergenic 45.0%
GCAACAGAAACCTGGAAAAG+AGG - chr2_4:38679587-38679606 Msa0327160:five_prime_UTR 45.0%
! GAGGACACACCTTTTATCAG+AGG + chr2_4:38679924-38679943 None:intergenic 45.0%
TCACATGTGTCATCTGGTGT+TGG + chr2_4:38679946-38679965 None:intergenic 45.0%
! CGATGTGTCATTATCGTGTC+CGG - chr2_4:38680001-38680020 Msa0327160:five_prime_UTR 45.0%
GCTTAAATTGCAGCTGCGTA+CGG - chr2_4:38680163-38680182 Msa0327160:five_prime_UTR 45.0%
! TCCGCAGATTCTAAGAGATG+AGG - chr2_4:38680471-38680490 Msa0327160:intron 45.0%
GCCTCATCTCTTAGAATCTG+CGG + chr2_4:38680475-38680494 None:intergenic 45.0%
! GATTCTAAGAGATGAGGCAG+TGG - chr2_4:38680477-38680496 Msa0327160:intron 45.0%
!! AAGGCTTGTTGAGCTTGGAA+AGG - chr2_4:38680501-38680520 Msa0327160:intron 45.0%
GAACAGGTTAGTGATGCTAG+TGG - chr2_4:38680768-38680787 Msa0327160:five_prime_UTR 45.0%
ATGCTAGTGGCTATCTAGAG+AGG - chr2_4:38680781-38680800 Msa0327160:five_prime_UTR 45.0%
TTTATCGCTGTTGTGGTTGC+AGG + chr2_4:38680819-38680838 None:intergenic 45.0%
CGAAAATGGATGGAAGATGC+TGG - chr2_4:38680846-38680865 Msa0327160:five_prime_UTR 45.0%
TTTCAGTTGGGTGGACCAAA+TGG - chr2_4:38680943-38680962 Msa0327160:five_prime_UTR 45.0%
TTCAGTTGGGTGGACCAAAT+GGG - chr2_4:38680944-38680963 Msa0327160:five_prime_UTR 45.0%
!! AAAAAAAAAAAAAAAAAAAG+TGG - chr2_4:38679788-38679807 Msa0327160:five_prime_UTR 5.0%
!! AAAAAAAAAAAAAAAAAAAG+TGG - chr2_4:38679788-38679807 Msa0327160:five_prime_UTR 5.0%
CATGCACGAGTGAGAAATGC+TGG + chr2_4:38679044-38679063 None:intergenic 50.0%
GAAAGAACAGCATGTGGTGG+AGG + chr2_4:38679094-38679113 None:intergenic 50.0%
TGTGTGTGTGTGTGTGGCTA+TGG + chr2_4:38679229-38679248 None:intergenic 50.0%
! TTCAGGTACGCCTCTTTTCC+AGG + chr2_4:38679600-38679619 None:intergenic 50.0%
!! GAGCTGGCGTTTGTTGATTC+AGG + chr2_4:38679617-38679636 None:intergenic 50.0%
! CATGTGTCATCTGGTGTTGG+AGG + chr2_4:38679943-38679962 None:intergenic 50.0%
TAAGAGATGAGGCAGTGGCA+AGG - chr2_4:38680482-38680501 Msa0327160:intron 50.0%
CATGCACGAGTGAGAAATGC+TGG + chr2_4:38679044-38679063 None:intergenic 50.0%
GAAAGAACAGCATGTGGTGG+AGG + chr2_4:38679094-38679113 None:intergenic 50.0%
TGTGTGTGTGTGTGTGGCTA+TGG + chr2_4:38679229-38679248 None:intergenic 50.0%
! TTCAGGTACGCCTCTTTTCC+AGG + chr2_4:38679600-38679619 None:intergenic 50.0%
!! GAGCTGGCGTTTGTTGATTC+AGG + chr2_4:38679617-38679636 None:intergenic 50.0%
! CATGTGTCATCTGGTGTTGG+AGG + chr2_4:38679943-38679962 None:intergenic 50.0%
TAAGAGATGAGGCAGTGGCA+AGG - chr2_4:38680482-38680501 Msa0327160:intron 50.0%
GCTGGACCCTACAAACTGAG+TGG + chr2_4:38679026-38679045 None:intergenic 55.0%
! AGAACAGCATGTGGTGGAGG+TGG + chr2_4:38679091-38679110 None:intergenic 55.0%
! TCCGGTGTCTGTGTCACTCA+GGG - chr2_4:38680019-38680038 Msa0327160:five_prime_UTR 55.0%
! CTGTGTCACTCAGGGCTTCA+TGG - chr2_4:38680027-38680046 Msa0327160:five_prime_UTR 55.0%
!! GTGGCAAGGCTTGTTGAGCT+TGG - chr2_4:38680496-38680515 Msa0327160:intron 55.0%
GCTGGACCCTACAAACTGAG+TGG + chr2_4:38679026-38679045 None:intergenic 55.0%
! AGAACAGCATGTGGTGGAGG+TGG + chr2_4:38679091-38679110 None:intergenic 55.0%
! TCCGGTGTCTGTGTCACTCA+GGG - chr2_4:38680019-38680038 Msa0327160:five_prime_UTR 55.0%
! CTGTGTCACTCAGGGCTTCA+TGG - chr2_4:38680027-38680046 Msa0327160:five_prime_UTR 55.0%
!! GTGGCAAGGCTTGTTGAGCT+TGG - chr2_4:38680496-38680515 Msa0327160:intron 55.0%
! ACAGCATGTGGTGGAGGTGG+CGG + chr2_4:38679088-38679107 None:intergenic 60.0%
! GTCCGGTGTCTGTGTCACTC+AGG - chr2_4:38680018-38680037 Msa0327160:five_prime_UTR 60.0%
GCCCTGAGTGACACAGACAC+CGG + chr2_4:38680023-38680042 None:intergenic 60.0%
! ACAGCATGTGGTGGAGGTGG+CGG + chr2_4:38679088-38679107 None:intergenic 60.0%
! GTCCGGTGTCTGTGTCACTC+AGG - chr2_4:38680018-38680037 Msa0327160:five_prime_UTR 60.0%
GCCCTGAGTGACACAGACAC+CGG + chr2_4:38680023-38680042 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 38678913 38680968 38678913 ID=Msa0327160;Name=Msa0327160
chr2_4 mRNA 38678913 38680968 38678913 ID=Msa0327160-mRNA-1;Parent=Msa0327160;Name=Msa0327160-mRNA-1;_AED=0.07;_eAED=0.07;_QI=1331|1|1|1|0.33|0|4|99|19
chr2_4 exon 38678913 38678932 38678913 ID=Msa0327160-mRNA-1:exon:11600;Parent=Msa0327160-mRNA-1
chr2_4 exon 38679004 38679107 38679004 ID=Msa0327160-mRNA-1:exon:11599;Parent=Msa0327160-mRNA-1
chr2_4 exon 38679359 38680302 38679359 ID=Msa0327160-mRNA-1:exon:11598;Parent=Msa0327160-mRNA-1
chr2_4 exon 38680547 38680968 38680547 ID=Msa0327160-mRNA-1:exon:11597;Parent=Msa0327160-mRNA-1
chr2_4 five_prime_UTR 38680547 38680968 38680547 ID=Msa0327160-mRNA-1:five_prime_utr;Parent=Msa0327160-mRNA-1
chr2_4 five_prime_UTR 38679394 38680302 38679394 ID=Msa0327160-mRNA-1:five_prime_utr;Parent=Msa0327160-mRNA-1
chr2_4 CDS 38679359 38679393 38679359 ID=Msa0327160-mRNA-1:cds;Parent=Msa0327160-mRNA-1
chr2_4 CDS 38679083 38679107 38679083 ID=Msa0327160-mRNA-1:cds;Parent=Msa0327160-mRNA-1
chr2_4 three_prime_UTR 38679004 38679082 38679004 ID=Msa0327160-mRNA-1:three_prime_utr;Parent=Msa0327160-mRNA-1
chr2_4 three_prime_UTR 38678913 38678932 38678913 ID=Msa0327160-mRNA-1:three_prime_utr;Parent=Msa0327160-mRNA-1
Gene Sequence

>Msa0327160

ATGAGGCAGTGGCAAGGCTTGTTGAGCTTGGAAAGGTTAGTGATGCTAGTGGCTATCTAG
Protein sequence

>Msa0327160

MRQWQGLLSLERLVMLVAI*