AlfalfaGEDB | Msa0328230

M. sativa cultivar zhongmu-4 / Msa0328230

KEGG: K20793
GO: -
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328230	XP_013463544.1	94.352	301	14	1	7	307	1	298	0.0	590

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0328230	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0328230	Msa0328280	0.833043	6.689459e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328230	MtrunA17_Chr2g0300621	94.352	301	14	1	7	307	1	298	0.0	590
Msa0328230	MtrunA17_Chr4g0073171	28.723	188	109	5	115	286	58	236	3.24e-12	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328230	AT2G06025.1	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328230	AT2G06025.6	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328230	AT2G06025.4	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328230	AT2G06025.3	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328230	AT2G06025.5	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328230	AT2G06025.2	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328230	AT2G06025.7	55.856	222	84	3	7	228	1	208	1.76e-81	246
Msa0328230	AT4G28030.1	30.583	206	101	7	111	292	58	245	4.16e-13	68.6

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Find 91 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCATCTTCCACAACTTT+AGG	0.268243	2_4:-40428606	None:intergenic
AAACTCTCGGTTGTTACCAG+AGG	0.674396	2_4:+40430865	Msa0328230:CDS
AAAGAAAATAGATTTGAGTT+TGG	0.287660	2_4:+40428684	Msa0328230:CDS
AAATTTATGTGTTGCCAAAT+TGG	0.283413	2_4:+40430523	Msa0328230:CDS
AAGAAAATAGATTTGAGTTT+GGG	0.347621	2_4:+40428685	Msa0328230:CDS
AAGCGGTGCAAGGTGCAAAA+TGG	0.311231	2_4:+40430035	Msa0328230:CDS
AAGTCAATGTATATGCGTGT+AGG	0.627047	2_4:+40431013	Msa0328230:three_prime_UTR
AATATCCGATATTTGCTGCA+AGG	0.560266	2_4:+40430328	Msa0328230:CDS
AATGAAACAGAACAAGAACA+GGG	0.696176	2_4:+40431158	Msa0328230:three_prime_UTR
AATTTGCAACTGTTCCAGAT+TGG	0.457980	2_4:-40430943	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATTGGAATAGTTGACAT+AGG	-	chr2_4:40428264-40428283	None:intergenic	30.0%
TCAAAAAGAAGTCAATCCAA+AGG	+	chr2_4:40428938-40428957	Msa0328230:intron	30.0%
CAAAAAGAAGTCAATCCAAA+GGG	+	chr2_4:40428939-40428958	Msa0328230:intron	30.0%
AGAACAAATCGACTAACTAA+GGG	-	chr2_4:40428965-40428984	None:intergenic	30.0%
AAGAACAAATCGACTAACTA+AGG	-	chr2_4:40428966-40428985	None:intergenic	30.0%
GTATTCAATTTGTCAGGATT+AGG	+	chr2_4:40429053-40429072	Msa0328230:intron	30.0%
TTTGTCAGGATTAGGTTAAT+TGG	+	chr2_4:40429061-40429080	Msa0328230:intron	30.0%
GACTATAAACCAAATCAAGA+TGG	+	chr2_4:40429682-40429701	Msa0328230:intron	30.0%
AGGAACAATAGGTAAGAATT+TGG	+	chr2_4:40429832-40429851	Msa0328230:intron	30.0%
GGAACAATAGGTAAGAATTT+GGG	+	chr2_4:40429833-40429852	Msa0328230:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	40428018	40431275	40428018	ID=Msa0328230;Name=Msa0328230
chr2_4	mRNA	40428018	40431275	40428018	ID=Msa0328230-mRNA-1;Parent=Msa0328230;Name=Msa0328230-mRNA-1;_AED=0.11;_eAED=0.11;_QI=228\|1\|1\|1\|1\|0.88\|9\|359\|308
chr2_4	exon	40428018	40428367	40428018	ID=Msa0328230-mRNA-1:exon:12350;Parent=Msa0328230-mRNA-1
chr2_4	exon	40428503	40428749	40428503	ID=Msa0328230-mRNA-1:exon:12351;Parent=Msa0328230-mRNA-1
chr2_4	exon	40429477	40429529	40429477	ID=Msa0328230-mRNA-1:exon:12352;Parent=Msa0328230-mRNA-1
chr2_4	exon	40429891	40429927	40429891	ID=Msa0328230-mRNA-1:exon:12353;Parent=Msa0328230-mRNA-1
chr2_4	exon	40430017	40430082	40430017	ID=Msa0328230-mRNA-1:exon:12354;Parent=Msa0328230-mRNA-1
chr2_4	exon	40430262	40430366	40430262	ID=Msa0328230-mRNA-1:exon:12355;Parent=Msa0328230-mRNA-1
chr2_4	exon	40430455	40430608	40430455	ID=Msa0328230-mRNA-1:exon:12356;Parent=Msa0328230-mRNA-1
chr2_4	exon	40430676	40430752	40430676	ID=Msa0328230-mRNA-1:exon:12357;Parent=Msa0328230-mRNA-1
chr2_4	exon	40430851	40431275	40430851	ID=Msa0328230-mRNA-1:exon:12358;Parent=Msa0328230-mRNA-1
chr2_4	five_prime_UTR	40428018	40428245	40428018	ID=Msa0328230-mRNA-1:five_prime_utr;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40428246	40428367	40428246	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40428503	40428749	40428503	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40429477	40429529	40429477	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40429891	40429927	40429891	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40430017	40430082	40430017	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40430262	40430366	40430262	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40430455	40430608	40430455	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40430676	40430752	40430676	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	CDS	40430851	40430916	40430851	ID=Msa0328230-mRNA-1:cds;Parent=Msa0328230-mRNA-1
chr2_4	three_prime_UTR	40430917	40431275	40430917	ID=Msa0328230-mRNA-1:three_prime_utr;Parent=Msa0328230-mRNA-1

Gene Sequence

>Msa0328230

ATGAAAATCATGTCACCTATGTCAACTATTCCAATTCATAAACCTGAATTTCATATCTTACTTTATAATTCATCTCCAACCCATAATTACAAATGCACCAAAATTACATCCTCATGGACCATGTCTATGGATTCTAAATTTTCTTCAACAATGAAGAGTAATAGTGATATGAAGAATAAGAAGATGAAGGAGGAACTTTCTGTGAAGCTTTCAAGCCCAATTGTTCCTAAAGTTGTGGAAGATGGTTTGAGCTCGAATGGTCTTCGATTTGATCGGTTGCAGCCGTCGGAAACAGAGTTGATTAAAGAAAATAGATTTGAGTTTGGGCAGTTTGTTGCTAGGGAAGCTGTGCTTGATGAAGAGTTTTGGACAGCAGCCTGGCTAAGGGCAGAAAGTCATTGGGAGAATCGAACATATGAAAGATATATTGATATCTACAAAAGGAAATTTGCTGATCAGGAATTTAATGCGGTAAAAAAGCGGTGCAAGGTGCAAAATGGGGACAGCTGCGCATGCATTATCGCGGTGAGAAAGGAGCAGAAGAACGTAAAGCGCTCAATAATAAAAAGTGTCGTAGGAACACTTGATTTGAATATCCGATATTTGCTGCAAGGAGAGACTTTCCCTGGGGAACGAGACAAAACTCCTTTTTGTTGCAGCATAAACAGAACACCACCAAGTAGATATGGTTACATTGCAAATTTATGTGTTGCCAAATTGGCTCGTCGACAAGGGATTGCAAGCAACATGATGTCTTTTGCTGTCGAAAATGCAAAATCTAATGGTGTGAGTCAGGTATATGTGCATGTTGACAGAAATAATAGGCCTGGACAGTTATTGTACCAAAAGATGGGCTTTGAGATGGTTGAAACTGCAAACTCTCGGTTGTTACCAGAGGAAACATACTTGCTACGTTTACAGACGTAA

Protein sequence

>Msa0328230

MKIMSPMSTIPIHKPEFHILLYNSSPTHNYKCTKITSSWTMSMDSKFSSTMKSNSDMKNKKMKEELSVKLSSPIVPKVVEDGLSSNGLRFDRLQPSETELIKENRFEFGQFVAREAVLDEEFWTAAWLRAESHWENRTYERYIDIYKRKFADQEFNAVKKRCKVQNGDSCACIIAVRKEQKNVKRSIIKSVVGTLDLNIRYLLQGETFPGERDKTPFCCSINRTPPSRYGYIANLCVAKLARRQGIASNMMSFAVENAKSNGVSQVYVHVDRNNRPGQLLYQKMGFEMVETANSRLLPEETYLLRLQT*