AlfalfaGEDB | Msa0328280

M. sativa cultivar zhongmu-4 / Msa0328280

KEGG: K20793
GO: -
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328280	XP_013463544.1	94.352	301	14	1	7	307	1	298	0.0	590

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0328280	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0328230	Msa0328280	0.833043	6.689459e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328280	MtrunA17_Chr2g0300621	94.352	301	14	1	7	307	1	298	0.0	590
Msa0328280	MtrunA17_Chr4g0073171	28.723	188	109	5	115	286	58	236	3.24e-12	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0328280	AT2G06025.1	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328280	AT2G06025.6	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328280	AT2G06025.4	58.194	299	111	3	7	305	1	285	9.61e-121	348
Msa0328280	AT2G06025.3	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328280	AT2G06025.5	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328280	AT2G06025.2	58.594	256	92	3	7	262	1	242	1.78e-102	300
Msa0328280	AT2G06025.7	55.856	222	84	3	7	228	1	208	1.76e-81	246
Msa0328280	AT4G28030.1	30.583	206	101	7	111	292	58	245	4.16e-13	68.6

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Find 91 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCATCTTCCACAACTTT+AGG	0.268243	2_4:+40488321	None:intergenic
AAACTCTCGGTTGTTACCAG+AGG	0.674396	2_4:-40486080	Msa0328280:CDS
AAAGAAAATAGATTTGAGTT+TGG	0.287660	2_4:-40488243	Msa0328280:CDS
AAATTTATGTGTTGCCAAAT+TGG	0.283413	2_4:-40486421	Msa0328280:CDS
AAGAAAATAGATTTGAGTTT+GGG	0.347621	2_4:-40488242	Msa0328280:CDS
AAGCGGTGCAAGGTGCAAAA+TGG	0.311231	2_4:-40486908	Msa0328280:CDS
AAGTCAATGTATATGCGTGT+AGG	0.627047	2_4:-40485933	Msa0328280:three_prime_UTR
AATATCCGATATTTGCTGCA+AGG	0.560266	2_4:-40486616	Msa0328280:CDS
AATGAAACAGAACAAGAACA+GGG	0.696176	2_4:-40485788	Msa0328280:three_prime_UTR
AATTTGCAACTGTTCCAGAT+TGG	0.457980	2_4:+40486003	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAATTGGAATAGTTGACAT+AGG	+	chr2_4:40485938-40485957	None:intergenic	30.0%
TCAAAAGAAGTCAATCCAAA+GGG	-	chr2_4:40486610-40486629	Msa0328280:CDS	30.0%
GAACAAATCGACATAACTAA+GGG	+	chr2_4:40486636-40486655	None:intergenic	30.0%
AGAACAAATCGACATAACTA+AGG	+	chr2_4:40486637-40486656	None:intergenic	30.0%
GTATTCAATTTGTCAGGATT+AGG	-	chr2_4:40486723-40486742	Msa0328280:intron	30.0%
TTTGTCAGGATTAGGTTAAT+TGG	-	chr2_4:40486731-40486750	Msa0328280:intron	30.0%
AGACTATAACCAAATCAAGA+TGG	-	chr2_4:40487345-40487364	Msa0328280:intron	30.0%
AGGAACAATAGGTAAGAATT+TGG	-	chr2_4:40487491-40487510	Msa0328280:intron	30.0%
GGAACAATAGGTAAGAATTT+GGG	-	chr2_4:40487492-40487511	Msa0328280:intron	30.0%
TAACAAGAATCCCTATGTTT+AGG	-	chr2_4:40487653-40487672	Msa0328280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	40485693	40488930	40485693	ID=Msa0328280;Name=Msa0328280
chr2_4	mRNA	40485693	40488930	40485693	ID=Msa0328280-mRNA-1;Parent=Msa0328280;Name=Msa0328280-mRNA-1;_AED=0.11;_eAED=0.11;_QI=227\|1\|1\|1\|1\|0.88\|9\|358\|308
chr2_4	exon	40485693	40486116	40485693	ID=Msa0328280-mRNA-1:exon:12395;Parent=Msa0328280-mRNA-1
chr2_4	exon	40486214	40486290	40486214	ID=Msa0328280-mRNA-1:exon:12394;Parent=Msa0328280-mRNA-1
chr2_4	exon	40486358	40486511	40486358	ID=Msa0328280-mRNA-1:exon:12393;Parent=Msa0328280-mRNA-1
chr2_4	exon	40486600	40486704	40486600	ID=Msa0328280-mRNA-1:exon:12392;Parent=Msa0328280-mRNA-1
chr2_4	exon	40486883	40486948	40486883	ID=Msa0328280-mRNA-1:exon:12391;Parent=Msa0328280-mRNA-1
chr2_4	exon	40487038	40487074	40487038	ID=Msa0328280-mRNA-1:exon:12390;Parent=Msa0328280-mRNA-1
chr2_4	exon	40487432	40487484	40487432	ID=Msa0328280-mRNA-1:exon:12389;Parent=Msa0328280-mRNA-1
chr2_4	exon	40488200	40488446	40488200	ID=Msa0328280-mRNA-1:exon:12388;Parent=Msa0328280-mRNA-1
chr2_4	exon	40488582	40488930	40488582	ID=Msa0328280-mRNA-1:exon:12387;Parent=Msa0328280-mRNA-1
chr2_4	five_prime_UTR	40488704	40488930	40488704	ID=Msa0328280-mRNA-1:five_prime_utr;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40488582	40488703	40488582	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40488200	40488446	40488200	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40487432	40487484	40487432	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40487038	40487074	40487038	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40486883	40486948	40486883	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40486600	40486704	40486600	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40486358	40486511	40486358	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40486214	40486290	40486214	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	CDS	40486051	40486116	40486051	ID=Msa0328280-mRNA-1:cds;Parent=Msa0328280-mRNA-1
chr2_4	three_prime_UTR	40485693	40486050	40485693	ID=Msa0328280-mRNA-1:three_prime_utr;Parent=Msa0328280-mRNA-1

Gene Sequence

>Msa0328280

ATGAAAATCATGTCACCTATGTCAACTATTCCAATTCATAAACCTGAATTTCATATCTTACTTTATAATTCATCTCCAACCCATAATTACAAATGCACCAAAATTACATCCTCATGGACCATGTCTATGGATTCTAAATTTTCTTCAACAATGAAGAGTAATAGTGATATGAAGAATAAGAAGATGAAGGAGGAACTTTCTGTGAAGCTTTCAAGCCCAATTGTTCCTAAAGTTGTGGAAGATGGTTTGAGCTCGAATGGTCTTCGATTTGATCGGTTGCAGCCGTCGGAAACAGAGTTGATTAAAGAAAATAGATTTGAGTTTGGGCAGTTTGTTGCTAGGGAAGCTGTGCTTGATGAAGAGTTTTGGACAGCAGCCTGGCTAAGGGCAGAAAGTCATTGGGAGAATCGAACATATGAAAGATATATTGATATCTACAAAAGGAAATTTGCTGATCAGGAATTTAATGCGGTAAAAAAGCGGTGCAAGGTGCAAAATGGGGACAGCTGCGCATGCATTATCGCGGTGAGAAAGGAGCAGAAGAACGTAAAGCGCTCAATAATAAAAAGTGTCGTAGGAACACTTGATTTGAATATCCGATATTTGCTGCAAGGAGAGACTTTCCCTGGGGAACGAGACAAAACTCCTTTTTGTTGCAGCATAAACAGAACACCACCAAGTAGATATGGTTACATTGCAAATTTATGTGTTGCCAAATTGGCTCGTCGACAAGGGATTGCAAGCAACATGATGTCTTTTGCTGTCGAAAATGCAAAATCTAATGGTGTGAGTCAGGTATATGTGCATGTTGACAGAAATAATAGGCCTGGACAGTTATTGTACCAAAAGATGGGCTTTGAGATGGTTGAAACTGCAAACTCTCGGTTGTTACCAGAGGAAACATACTTGCTACGTTTACAGACGTAA

Protein sequence

>Msa0328280

MKIMSPMSTIPIHKPEFHILLYNSSPTHNYKCTKITSSWTMSMDSKFSSTMKSNSDMKNKKMKEELSVKLSSPIVPKVVEDGLSSNGLRFDRLQPSETELIKENRFEFGQFVAREAVLDEEFWTAAWLRAESHWENRTYERYIDIYKRKFADQEFNAVKKRCKVQNGDSCACIIAVRKEQKNVKRSIIKSVVGTLDLNIRYLLQGETFPGERDKTPFCCSINRTPPSRYGYIANLCVAKLARRQGIASNMMSFAVENAKSNGVSQVYVHVDRNNRPGQLLYQKMGFEMVETANSRLLPEETYLLRLQT*