AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0330000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 337 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGTTGTCCTAACAATTA+AGG 0.130811 2_4:+44000760 None:intergenic
TTTCAAAATTCGCATGAAAA+TGG 0.247579 2_4:+44000614 None:intergenic
TCAGTTTACCCTTAATTGTT+AGG 0.300983 2_4:-44000769 Msa0330000:three_prime_UTR
CATGATATGTTAGCCTGTTC+TGG 0.307236 2_4:-44000994 Msa0330000:three_prime_UTR
TAGATCTCTGTCTCATCTTT+CGG 0.343914 2_4:+44001068 None:intergenic
AAACTGAAAGCATAAATAAA+AGG 0.357989 2_4:+44000785 None:intergenic
TCTGTTGTCCTAACAATTAA+GGG 0.369557 2_4:+44000761 None:intergenic
TATTGGTGCATGTACTGTTT+TGG 0.389852 2_4:-44000875 Msa0330000:three_prime_UTR
GATCTTGAGGTTTGGTTGGG+AGG 0.398156 2_4:-44000965 Msa0330000:three_prime_UTR
TTGAAGGAAGTAGCTTGAAA+TGG 0.399068 2_4:-44006284 Msa0330000:CDS
TCTTGAGGTTTGGTTGGGAG+GGG 0.402117 2_4:-44000963 Msa0330000:three_prime_UTR
ATCTTGAGGTTTGGTTGGGA+GGG 0.418305 2_4:-44000964 Msa0330000:three_prime_UTR
CTTTCCATCTTGCTTGATGC+CGG 0.419505 2_4:-44001020 Msa0330000:three_prime_UTR
GCTACTTCCTTCAAGAATCA+TGG 0.427886 2_4:+44006293 None:intergenic
TATTGATCTTGAGGTTTGGT+TGG 0.431962 2_4:-44000969 Msa0330000:three_prime_UTR
GGTGTATTGATCTTGAGGTT+TGG 0.447872 2_4:-44000973 Msa0330000:three_prime_UTR
CAAGATCAATACACCAGAAC+AGG 0.455956 2_4:+44000981 None:intergenic
ATTGATCTTGAGGTTTGGTT+GGG 0.481359 2_4:-44000968 Msa0330000:three_prime_UTR
TCACAAACCATGATTCTTGA+AGG 0.482297 2_4:-44006300 Msa0330000:CDS
ATGAAAATCATGTGGATGAG+GGG 0.488835 2_4:-44000649 Msa0330000:three_prime_UTR
TATCAATCTCTTCTAAAAGC+AGG 0.489555 2_4:-44006333 Msa0330000:CDS
GTTCTGGTGTATTGATCTTG+AGG 0.502598 2_4:-44000978 Msa0330000:three_prime_UTR
AGCGTATGAGGCGGTTGAAG+AGG 0.522163 2_4:-44001096 Msa0330000:CDS
GATGAGACAGAGATCTAAGT+AGG 0.529953 2_4:-44001062 Msa0330000:exon
ATCTAAGTAGGTGCATATGC+AGG 0.532333 2_4:-44001050 Msa0330000:exon
AGTGGAAGAAGAAGCGTATG+AGG 0.535090 2_4:-44001108 Msa0330000:CDS
ACATGAAAATCATGTGGATG+AGG 0.537564 2_4:-44000651 Msa0330000:three_prime_UTR
CATGAAAATCATGTGGATGA+GGG 0.543834 2_4:-44000650 Msa0330000:three_prime_UTR
CACTGGGTTAGCTTAAGATG+TGG 0.544623 2_4:-44000712 Msa0330000:three_prime_UTR
GGAAGTAGCTTGAAATGGAT+TGG 0.553540 2_4:-44006279 Msa0330000:CDS
TCAAGAATCATGGTTTGTGA+TGG 0.567136 2_4:+44006303 None:intergenic
GAAGCCGGCATCAAGCAAGA+TGG 0.582153 2_4:+44001016 None:intergenic
AGGCTAACATATCATGAAGC+CGG 0.585518 2_4:+44001001 None:intergenic
TGATAGCAAGAAGTAAGCAT+TGG 0.587420 2_4:+44006351 None:intergenic
ATCTTAAGCTAACCCAGTGA+AGG 0.623767 2_4:+44000716 None:intergenic
TGAAAATCATGTGGATGAGG+GGG 0.631920 2_4:-44000648 Msa0330000:three_prime_UTR
AGCTTGAAATGGATTGGTGA+TGG 0.636485 2_4:-44006273 Msa0330000:intron
CTGAAAGCATAAATAAAAGG+AGG 0.640654 2_4:+44000788 None:intergenic
TTAAAGACATGAAAATCATG+TGG 0.661283 2_4:-44000657 Msa0330000:three_prime_UTR
TCAATTGTCTTAGAACAACA+AGG 0.700588 2_4:+44000915 None:intergenic
GGAAGAAGAAGCGTATGAGG+CGG 0.708289 2_4:-44001105 Msa0330000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATATTTGATTTTATCA+CGG - chr2_4:44002006-44002025 Msa0330000:intron 10.0%
!!! ATGTTATTTATGTTTAATTT+TGG - chr2_4:44002777-44002796 Msa0330000:intron 10.0%
!!! TGTTTATTAAATTTTATTCT+TGG - chr2_4:44003191-44003210 Msa0330000:intron 10.0%
!! ATAACCATTATATTATAATA+GGG + chr2_4:44003320-44003339 None:intergenic 10.0%
!!! TTTTAAATGTTCAATATTTT+TGG - chr2_4:44004819-44004838 Msa0330000:intron 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG - chr2_4:44004820-44004839 Msa0330000:intron 10.0%
!!! GTTTAAGAAGATATTATTTT+AGG + chr2_4:44001826-44001845 None:intergenic 15.0%
!!! TTTTAATTTGTCTTTATGTA+AGG + chr2_4:44001991-44002010 None:intergenic 15.0%
!!! AAAATATTTGATTTTATCAC+GGG - chr2_4:44002007-44002026 Msa0330000:intron 15.0%
!!! AGAAAATTTCTAAAAAAAGT+GGG + chr2_4:44002111-44002130 None:intergenic 15.0%
!!! TTTATTACATATTAGGATTT+TGG - chr2_4:44002539-44002558 Msa0330000:intron 15.0%
!!! AAATTCTAAGAAATTTTCTA+GGG + chr2_4:44002614-44002633 None:intergenic 15.0%
!!! AAAATTCTAAGAAATTTTCT+AGG + chr2_4:44002615-44002634 None:intergenic 15.0%
!! GATAACCATTATATTATAAT+AGG + chr2_4:44003321-44003340 None:intergenic 15.0%
!! TTATAGTACAACTATTAATT+GGG + chr2_4:44003602-44003621 None:intergenic 15.0%
!! TTTATAGTACAACTATTAAT+TGG + chr2_4:44003603-44003622 None:intergenic 15.0%
!!! ATTAAATAGAGAAAAACTTT+TGG - chr2_4:44004088-44004107 Msa0330000:intron 15.0%
!!! ATTTTGAAATTTTGATACAT+TGG + chr2_4:44004165-44004184 None:intergenic 15.0%
!! ATAATAAATAAGCATATAGA+GGG - chr2_4:44004306-44004325 Msa0330000:intron 15.0%
!! GATACATTACAAAATTATTA+TGG - chr2_4:44004417-44004436 Msa0330000:intron 15.0%
!! ATTAGATACAATTATGATTT+AGG - chr2_4:44004843-44004862 Msa0330000:intron 15.0%
!! TTAGATACAATTATGATTTA+GGG - chr2_4:44004844-44004863 Msa0330000:intron 15.0%
!!! AGTTAATTTGATGTTATTTT+AGG - chr2_4:44005050-44005069 Msa0330000:intron 15.0%
!!! TTAAAAAGTGATTAATTTGA+TGG - chr2_4:44005210-44005229 Msa0330000:intron 15.0%
!!! GATTCAATTTTTGATTACTT+TGG + chr2_4:44001204-44001223 None:intergenic 20.0%
!!! TTTTTTCAAGGGTTTTTAAA+AGG + chr2_4:44001421-44001440 None:intergenic 20.0%
!!! ATAATTTCTCGTTTTTTCAA+GGG + chr2_4:44001432-44001451 None:intergenic 20.0%
!!! AATAATTTCTCGTTTTTTCA+AGG + chr2_4:44001433-44001452 None:intergenic 20.0%
!! CGAGAAATTATTAGTAATAA+AGG - chr2_4:44001441-44001460 Msa0330000:intron 20.0%
!!! AACTTGAATTTTTTACACAT+TGG + chr2_4:44001895-44001914 None:intergenic 20.0%
!! TTCTAATATCACAAAATTAC+TGG - chr2_4:44001914-44001933 Msa0330000:intron 20.0%
!!! GAGAAAATTTCTAAAAAAAG+TGG + chr2_4:44002112-44002131 None:intergenic 20.0%
!! AATCGTTTATTATATCTGAA+GGG + chr2_4:44002195-44002214 None:intergenic 20.0%
!! TAATCGTTTATTATATCTGA+AGG + chr2_4:44002196-44002215 None:intergenic 20.0%
!! ATATAACTACTTAGTATAAC+TGG - chr2_4:44002579-44002598 Msa0330000:intron 20.0%
!!! TTTATATTTAGCCCATTTTT+TGG - chr2_4:44002637-44002656 Msa0330000:intron 20.0%
!! AATTCATATTACGATTGTAA+CGG + chr2_4:44002746-44002765 None:intergenic 20.0%
!! CTTATAAATTTAAGTCTAGT+TGG + chr2_4:44003094-44003113 None:intergenic 20.0%
!! AAGTTATCTTTATATATTCC+TGG - chr2_4:44003126-44003145 Msa0330000:intron 20.0%
!!! ATTTTTGTCATTTTATTACC+AGG + chr2_4:44003147-44003166 None:intergenic 20.0%
!!! TTTTTTCCATCTTTTTCTTT+GGG + chr2_4:44003248-44003267 None:intergenic 20.0%
!!! TTTTTTTCCATCTTTTTCTT+TGG + chr2_4:44003249-44003268 None:intergenic 20.0%
!! ATTATAATAGGGATGTTAAT+GGG + chr2_4:44003309-44003328 None:intergenic 20.0%
!! TATTATAATAGGGATGTTAA+TGG + chr2_4:44003310-44003329 None:intergenic 20.0%
!! ACATCCCTATTATAATATAA+TGG - chr2_4:44003313-44003332 Msa0330000:intron 20.0%
!!! GCTATTAATTTTTTTTTTCC+GGG + chr2_4:44003388-44003407 None:intergenic 20.0%
!!! GGCTATTAATTTTTTTTTTC+CGG + chr2_4:44003389-44003408 None:intergenic 20.0%
!!! TTGTTTAAAATCTCACAAAA+TGG + chr2_4:44003418-44003437 None:intergenic 20.0%
!!! TGAGATTTTAAACAATTTTG+CGG - chr2_4:44003423-44003442 Msa0330000:intron 20.0%
!!! TTACAAATCTTATAATTTGC+AGG - chr2_4:44003532-44003551 Msa0330000:intron 20.0%
!! TAATTGATAGAACAATGTAA+GGG + chr2_4:44003570-44003589 None:intergenic 20.0%
!! TTAATTGATAGAACAATGTA+AGG + chr2_4:44003571-44003590 None:intergenic 20.0%
!! TATAGTACAACTATTAATTG+GGG + chr2_4:44003601-44003620 None:intergenic 20.0%
!!! TAAATTGTAGCTTATTTTAG+TGG - chr2_4:44003754-44003773 Msa0330000:intron 20.0%
!!! TCATACTTTTTTTTTTCTTC+TGG - chr2_4:44003782-44003801 Msa0330000:intron 20.0%
!! GTTGATATTTATATATTTCC+AGG + chr2_4:44003819-44003838 None:intergenic 20.0%
!!! TTTTCGTTCAAATTATTTAG+TGG - chr2_4:44003922-44003941 Msa0330000:intron 20.0%
!! GATAATAAATAAGCATATAG+AGG - chr2_4:44004305-44004324 Msa0330000:intron 20.0%
!! CAAATAATATCATTTCTATG+AGG + chr2_4:44004941-44004960 None:intergenic 20.0%
!! TAAATTAGATGTGAATGTAT+TGG - chr2_4:44005355-44005374 Msa0330000:intron 20.0%
!! TTACTTATTGTACATGTTAT+AGG - chr2_4:44005419-44005438 Msa0330000:intron 20.0%
!! TACTTATTGTACATGTTATA+GGG - chr2_4:44005420-44005439 Msa0330000:intron 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG + chr2_4:44006204-44006223 None:intergenic 20.0%
!!! GATATAGGAATTTTAGCAAT+TGG + chr2_4:44000974-44000993 None:intergenic 25.0%
!!! TAAGTGATAATGGTATATCT+TGG + chr2_4:44001031-44001050 None:intergenic 25.0%
! TCTAATCATCTAAGTGATAA+TGG + chr2_4:44001041-44001060 None:intergenic 25.0%
! AAAACGGAAAATTATCCATT+CGG - chr2_4:44001570-44001589 Msa0330000:intron 25.0%
! CAACACTTTCAAAAAATTTC+AGG - chr2_4:44001845-44001864 Msa0330000:intron 25.0%
!! AAATTTCTAAAAAAAGTGGG+TGG + chr2_4:44002108-44002127 None:intergenic 25.0%
! CTATGCATATATAAGAGATT+AGG - chr2_4:44002288-44002307 Msa0330000:intron 25.0%
! ATAAACTTACCTTTAATCAG+AGG + chr2_4:44002338-44002357 None:intergenic 25.0%
! TTAAAACAACCGCTATAAAA+GGG + chr2_4:44002669-44002688 None:intergenic 25.0%
! ATTAAAACAACCGCTATAAA+AGG + chr2_4:44002670-44002689 None:intergenic 25.0%
! ATTGTAACGGTCATTATAAA+AGG + chr2_4:44002733-44002752 None:intergenic 25.0%
!! TATGTCTCATGAATTGTTTT+TGG - chr2_4:44002908-44002927 Msa0330000:intron 25.0%
!!! ATGTCTCATGAATTGTTTTT+GGG - chr2_4:44002909-44002928 Msa0330000:intron 25.0%
! CTTGGATATGAAATATTCAT+AGG - chr2_4:44003209-44003228 Msa0330000:intron 25.0%
!! TTTTTCCATCTTTTTCTTTG+GGG + chr2_4:44003247-44003266 None:intergenic 25.0%
! GGATTTATTCTAATCTTCTT+AGG + chr2_4:44003275-44003294 None:intergenic 25.0%
!! TGTTAATGGGTAAATATACA+TGG + chr2_4:44003296-44003315 None:intergenic 25.0%
!!! TTAATTTTTTTTTTCCGGGT+GGG + chr2_4:44003384-44003403 None:intergenic 25.0%
!!! ATTAATTTTTTTTTTCCGGG+TGG + chr2_4:44003385-44003404 None:intergenic 25.0%
! ATAGTACAACTATTAATTGG+GGG + chr2_4:44003600-44003619 None:intergenic 25.0%
!! AAAGAGGACAATTTTATTAC+TGG + chr2_4:44003866-44003885 None:intergenic 25.0%
! ACTTTCTGATGAATATAAAG+AGG + chr2_4:44003882-44003901 None:intergenic 25.0%
!!! TTGAAATTTTGATACATTGG+AGG + chr2_4:44004162-44004181 None:intergenic 25.0%
!!! CTGAAAGATTATTTTAACGT+TGG + chr2_4:44004217-44004236 None:intergenic 25.0%
!! ATTTGGGAACAAAAAGTTTT+CGG + chr2_4:44004471-44004490 None:intergenic 25.0%
!! CTAGTTGTTATTGATATCAA+AGG + chr2_4:44004543-44004562 None:intergenic 25.0%
!! TACAATTATGATTTAGGGTT+TGG - chr2_4:44004849-44004868 Msa0330000:intron 25.0%
!! ACAATTATGATTTAGGGTTT+GGG - chr2_4:44004850-44004869 Msa0330000:intron 25.0%
! ATAAGTAAAAAAGACAATGC+TGG + chr2_4:44005407-44005426 None:intergenic 25.0%
! CATACACATTACAAAACATA+AGG + chr2_4:44005547-44005566 None:intergenic 25.0%
! TTCATAACAGTATCATTCTT+TGG - chr2_4:44005762-44005781 Msa0330000:intron 25.0%
! TTAAAGACATGAAAATCATG+TGG - chr2_4:44006329-44006348 Msa0330000:CDS 25.0%
! TATCAATCTCTTCTAAAAGC+AGG - chr2_4:44000653-44000672 Msa0330000:three_prime_UTR 30.0%
GCTCATGAAAATGAACATTT+AGG - chr2_4:44000814-44000833 Msa0330000:three_prime_UTR 30.0%
! AATGAACATTTAGGATCACA+AGG - chr2_4:44000823-44000842 Msa0330000:three_prime_UTR 30.0%
! TTAATCACGTTTAAGACCAA+GGG + chr2_4:44000884-44000903 None:intergenic 30.0%
CATGTTTGTGTTAACATCAA+GGG + chr2_4:44001122-44001141 None:intergenic 30.0%
TCATGTTTGTGTTAACATCA+AGG + chr2_4:44001123-44001142 None:intergenic 30.0%
ATGTTAACACAAACATGATG+CGG - chr2_4:44001125-44001144 Msa0330000:intron 30.0%
TGATTACTTTGGATAAGTCA+TGG + chr2_4:44001193-44001212 None:intergenic 30.0%
CTTCCAACTTGAAATTGTAA+AGG - chr2_4:44001223-44001242 Msa0330000:intron 30.0%
! ACACCTTTACAATTTCAAGT+TGG + chr2_4:44001229-44001248 None:intergenic 30.0%
AAGGACATTTCTTGAAAAGA+TGG - chr2_4:44001352-44001371 Msa0330000:intron 30.0%
ATAAATAATCACACTCGCTA+TGG - chr2_4:44001482-44001501 Msa0330000:intron 30.0%
AATCTTGTCAACGATATCAA+AGG - chr2_4:44001653-44001672 Msa0330000:intron 30.0%
ATAGATGTCTCATAAGTGAA+AGG + chr2_4:44002043-44002062 None:intergenic 30.0%
AGATAAAGTCAAAGAAGAGA+GGG + chr2_4:44002082-44002101 None:intergenic 30.0%
AGGATTGTTAACAAGATTGT+AGG + chr2_4:44002318-44002337 None:intergenic 30.0%
GTTAACAATCCTCTGATTAA+AGG - chr2_4:44002326-44002345 Msa0330000:intron 30.0%
!!! AGCGGTTGTTTTAATATAAC+TGG - chr2_4:44002675-44002694 Msa0330000:intron 30.0%
!!! TGTCTCATGAATTGTTTTTG+GGG - chr2_4:44002910-44002929 Msa0330000:intron 30.0%
AGTTACACGTCAATCATATT+AGG - chr2_4:44003068-44003087 Msa0330000:intron 30.0%
AATATCCCCAAAGAAAAAGA+TGG - chr2_4:44003239-44003258 Msa0330000:intron 30.0%
AATATAATGGTTATCGACAG+TGG - chr2_4:44003326-44003345 Msa0330000:intron 30.0%
AATCTCACAAAATGGACATA+TGG + chr2_4:44003410-44003429 None:intergenic 30.0%
! GATTTTAAACAATTTTGCGG+CGG - chr2_4:44003426-44003445 Msa0330000:intron 30.0%
CAATAGTCATTGAATGTGTA+AGG + chr2_4:44004448-44004467 None:intergenic 30.0%
! AGTGTTTTTCCAAGCAATTT+GGG + chr2_4:44004487-44004506 None:intergenic 30.0%
! TAGTGTTTTTCCAAGCAATT+TGG + chr2_4:44004488-44004507 None:intergenic 30.0%
AAAACACTAAAATTGCACGA+TGG - chr2_4:44004499-44004518 Msa0330000:intron 30.0%
ATATCAATAACAACTAGACC+CGG - chr2_4:44004546-44004565 Msa0330000:intron 30.0%
CACTATAAATTCGAAAACGA+GGG - chr2_4:44004629-44004648 Msa0330000:intron 30.0%
!!! TTTTAGTTTGTTGTTGTCGT+TGG - chr2_4:44005023-44005042 Msa0330000:intron 30.0%
CAAAACATAAGGTTACAACA+TGG + chr2_4:44005536-44005555 None:intergenic 30.0%
ACTTAAAAACCACAAAATGC+TGG + chr2_4:44005605-44005624 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG - chr2_4:44005605-44005624 Msa0330000:intron 30.0%
! AGTCATGTTACAAGTTTGTT+TGG - chr2_4:44005722-44005741 Msa0330000:intron 30.0%
CTGTTATGAAGCAATGTTAA+TGG + chr2_4:44005752-44005771 None:intergenic 30.0%
!!! TATGTATTTTTTTCCTGTCG+TGG - chr2_4:44005795-44005814 Msa0330000:intron 30.0%
TGAATGATGTTATGTTTCAG+TGG - chr2_4:44005860-44005879 Msa0330000:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG + chr2_4:44006074-44006093 None:intergenic 30.0%
! TTTTGAGCGTTTTGTAGTAT+TGG - chr2_4:44006094-44006113 Msa0330000:intron 30.0%
! CTGAAAGCATAAATAAAAGG+AGG + chr2_4:44006201-44006220 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG - chr2_4:44006217-44006236 Msa0330000:intron 30.0%
TCTGTTGTCCTAACAATTAA+GGG + chr2_4:44006228-44006247 None:intergenic 30.0%
ATCTGTTGTCCTAACAATTA+AGG + chr2_4:44006229-44006248 None:intergenic 30.0%
! ATTTTATGATATGCCTTCAC+TGG - chr2_4:44006257-44006276 Msa0330000:intron 30.0%
! TTTTATGATATGCCTTCACT+GGG - chr2_4:44006258-44006277 Msa0330000:intron 30.0%
TGATAGCAAGAAGTAAGCAT+TGG + chr2_4:44000638-44000657 None:intergenic 35.0%
! TCAAGAATCATGGTTTGTGA+TGG + chr2_4:44000686-44000705 None:intergenic 35.0%
TCACAAACCATGATTCTTGA+AGG - chr2_4:44000686-44000705 Msa0330000:three_prime_UTR 35.0%
TTGAAGGAAGTAGCTTGAAA+TGG - chr2_4:44000702-44000721 Msa0330000:three_prime_UTR 35.0%
AGCATTTCTCTCTTTCTCTA+AGG + chr2_4:44000797-44000816 None:intergenic 35.0%
TGAAAATACCATATTGCCCT+TGG - chr2_4:44000865-44000884 Msa0330000:three_prime_UTR 35.0%
! GTTAATCACGTTTAAGACCA+AGG + chr2_4:44000885-44000904 None:intergenic 35.0%
AAATTCCTATATCTGACGAC+TGG - chr2_4:44000981-44001000 Msa0330000:three_prime_UTR 35.0%
CATCAAGGGAACTTATTTAG+AGG + chr2_4:44001108-44001127 None:intergenic 35.0%
! ACTCCATTTGGTTCATATGA+AGG - chr2_4:44001285-44001304 Msa0330000:intron 35.0%
! CTCCATTTGGTTCATATGAA+GGG - chr2_4:44001286-44001305 Msa0330000:intron 35.0%
TTCCCTTCATATGAACCAAA+TGG + chr2_4:44001291-44001310 None:intergenic 35.0%
TCCAACATACAGTAAGCAAA+AGG - chr2_4:44001333-44001352 Msa0330000:intron 35.0%
! TCCTTTTGCTTACTGTATGT+TGG + chr2_4:44001337-44001356 None:intergenic 35.0%
GAAAAGATGGTCTAGACTTT+CGG - chr2_4:44001365-44001384 Msa0330000:intron 35.0%
GCGAGTGTGATTATTTATGT+GGG + chr2_4:44001480-44001499 None:intergenic 35.0%
AGCGAGTGTGATTATTTATG+TGG + chr2_4:44001481-44001500 None:intergenic 35.0%
AGTCAATAATACTTGCCTGA+TGG + chr2_4:44001520-44001539 None:intergenic 35.0%
! AGGCAAGTATTATTGACTGT+TGG - chr2_4:44001522-44001541 Msa0330000:intron 35.0%
! CAAGTATTATTGACTGTTGG+AGG - chr2_4:44001525-44001544 Msa0330000:intron 35.0%
! TTTTATGATGCACACATCGT+TGG + chr2_4:44001553-44001572 None:intergenic 35.0%
CGATGTGTGCATCATAAAAA+CGG - chr2_4:44001554-44001573 Msa0330000:intron 35.0%
CAACGATATCAAAGGAAAGA+TGG - chr2_4:44001661-44001680 Msa0330000:intron 35.0%
! TTGTGAGAGATAATGGTGAT+TGG + chr2_4:44001707-44001726 None:intergenic 35.0%
! CTAAGAATCTTTTCCCTCTT+CGG + chr2_4:44001776-44001795 None:intergenic 35.0%
GAGATAAAGTCAAAGAAGAG+AGG + chr2_4:44002083-44002102 None:intergenic 35.0%
! TTTCTAAAAAAAGTGGGTGG+TGG + chr2_4:44002105-44002124 None:intergenic 35.0%
AATGAAGAGGAATAGATGGA+GGG + chr2_4:44002134-44002153 None:intergenic 35.0%
AGGGAAAAGATGATATGAGT+GGG + chr2_4:44002176-44002195 None:intergenic 35.0%
AAGGGAAAAGATGATATGAG+TGG + chr2_4:44002177-44002196 None:intergenic 35.0%
TTCCTCATACAAGCTTTCAT+AGG + chr2_4:44002255-44002274 None:intergenic 35.0%
GAAAGTTCATCTCAAAAGAG+CGG + chr2_4:44002381-44002400 None:intergenic 35.0%
AAGGGCTGAAACCAAAAAAT+GGG + chr2_4:44002651-44002670 None:intergenic 35.0%
AAAGGGCTGAAACCAAAAAA+TGG + chr2_4:44002652-44002671 None:intergenic 35.0%
CTAAATAAAACACAACGCAG+AGG + chr2_4:44002834-44002853 None:intergenic 35.0%
! TTGGGGTGACTTTAGATTTT+AGG - chr2_4:44002927-44002946 Msa0330000:intron 35.0%
! TGGGGTGACTTTAGATTTTA+GGG - chr2_4:44002928-44002947 Msa0330000:intron 35.0%
GCTTATACTTGACAAGTTAG+CGG - chr2_4:44003505-44003524 Msa0330000:intron 35.0%
CATTAAGCACAAAGTTGAAG+TGG - chr2_4:44003659-44003678 Msa0330000:intron 35.0%
CATTAAGCACAAAGTTGAAG+TGG + chr2_4:44003659-44003678 None:intergenic 35.0%
TATATATTTCCAGGAACCAG+TGG + chr2_4:44003810-44003829 None:intergenic 35.0%
!!! GGAAACTATTTTGACTAACG+AGG + chr2_4:44004196-44004215 None:intergenic 35.0%
! CTTTTTGTTCCCAAATTGCT+TGG - chr2_4:44004475-44004494 Msa0330000:intron 35.0%
AATTGCACGATGGCAATAAT+TGG - chr2_4:44004509-44004528 Msa0330000:intron 35.0%
ATTGCACGATGGCAATAATT+GGG - chr2_4:44004510-44004529 Msa0330000:intron 35.0%
TATCAATAACAACTAGACCC+GGG - chr2_4:44004547-44004566 Msa0330000:intron 35.0%
GCACTATAAATTCGAAAACG+AGG - chr2_4:44004628-44004647 Msa0330000:intron 35.0%
! AATTACCTTAGCTCTCATGT+TGG + chr2_4:44004744-44004763 None:intergenic 35.0%
! TAGGGTTTGGGTTTAGATTT+CGG - chr2_4:44004862-44004881 Msa0330000:intron 35.0%
CAAACTAAAACAACCAACCA+TGG + chr2_4:44005013-44005032 None:intergenic 35.0%
! TACTATAATCTGAGATGCAC+TGG - chr2_4:44005315-44005334 Msa0330000:intron 35.0%
GTAGAATAACAGTTAATGGC+AGG + chr2_4:44005385-44005404 None:intergenic 35.0%
GCTGGTAGAATAACAGTTAA+TGG + chr2_4:44005389-44005408 None:intergenic 35.0%
TTGTACCATTATGATGCTTG+TGG - chr2_4:44005445-44005464 Msa0330000:intron 35.0%
TGTACCATTATGATGCTTGT+GGG - chr2_4:44005446-44005465 Msa0330000:intron 35.0%
TGGGATTGTGTGATTTCTTA+AGG - chr2_4:44005465-44005484 Msa0330000:intron 35.0%
! GTTTTGTAATGTGTATGCTG+AGG - chr2_4:44005550-44005569 Msa0330000:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG + chr2_4:44005921-44005940 None:intergenic 35.0%
! TATTGATCTTGAGGTTTGGT+TGG - chr2_4:44006017-44006036 Msa0330000:intron 35.0%
! ATTGATCTTGAGGTTTGGTT+GGG - chr2_4:44006018-44006037 Msa0330000:intron 35.0%
! TATTGGTGCATGTACTGTTT+TGG - chr2_4:44006111-44006130 Msa0330000:intron 35.0%
ACATGAAAATCATGTGGATG+AGG - chr2_4:44006335-44006354 Msa0330000:CDS 35.0%
CATGAAAATCATGTGGATGA+GGG - chr2_4:44006336-44006355 Msa0330000:CDS 35.0%
ATGAAAATCATGTGGATGAG+GGG - chr2_4:44006337-44006356 Msa0330000:CDS 35.0%
GCTACTTCCTTCAAGAATCA+TGG + chr2_4:44000696-44000715 None:intergenic 40.0%
GGAAGTAGCTTGAAATGGAT+TGG - chr2_4:44000707-44000726 Msa0330000:three_prime_UTR 40.0%
!! AGCTTGAAATGGATTGGTGA+TGG - chr2_4:44000713-44000732 Msa0330000:three_prime_UTR 40.0%
! GGATCACAAGGCACTTATAT+AGG - chr2_4:44000835-44000854 Msa0330000:three_prime_UTR 40.0%
! GGCAATATGGTATTTTCACG+AGG + chr2_4:44000863-44000882 None:intergenic 40.0%
! GTTTAAGACCAAGGGCAATA+TGG + chr2_4:44000876-44000895 None:intergenic 40.0%
! ATGTTTTATCGTGCAGTGTC+AGG + chr2_4:44000907-44000926 None:intergenic 40.0%
GTTCAGGGATTCAATTACGA+CGG + chr2_4:44000933-44000952 None:intergenic 40.0%
CAAGGGAACTTATTTAGAGG+TGG + chr2_4:44001105-44001124 None:intergenic 40.0%
! ATGGCATGCAAGGAACATTT+TGG + chr2_4:44001174-44001193 None:intergenic 40.0%
TTCAACCTGTCAACTCCATT+TGG - chr2_4:44001273-44001292 Msa0330000:intron 40.0%
ATGAACCAAATGGAGTTGAC+AGG + chr2_4:44001281-44001300 None:intergenic 40.0%
ACTGTATGTTGGACTTCCTT+GGG + chr2_4:44001326-44001345 None:intergenic 40.0%
TACTGTATGTTGGACTTCCT+TGG + chr2_4:44001327-44001346 None:intergenic 40.0%
AGGAGAGGATGATATTGCTT+GGG + chr2_4:44001604-44001623 None:intergenic 40.0%
TAGGAGAGGATGATATTGCT+TGG + chr2_4:44001605-44001624 None:intergenic 40.0%
!! ACTATCATACGCTTTAGGAG+AGG + chr2_4:44001619-44001638 None:intergenic 40.0%
TCTCCTAAAGCGTATGATAG+TGG - chr2_4:44001618-44001637 Msa0330000:intron 40.0%
CTCCTAAAGCGTATGATAGT+GGG - chr2_4:44001619-44001638 Msa0330000:intron 40.0%
ATCCCACTATCATACGCTTT+AGG + chr2_4:44001624-44001643 None:intergenic 40.0%
!! TGTCGCTTTGTGAGAGATAA+TGG + chr2_4:44001714-44001733 None:intergenic 40.0%
AAAGTCAAAGAAGAGAGGGA+AGG + chr2_4:44002078-44002097 None:intergenic 40.0%
CAATGAAGAGGAATAGATGG+AGG + chr2_4:44002135-44002154 None:intergenic 40.0%
AGGCAATGAAGAGGAATAGA+TGG + chr2_4:44002138-44002157 None:intergenic 40.0%
GGGAAAAGATGATATGAGTG+GGG + chr2_4:44002175-44002194 None:intergenic 40.0%
TGCCTATGAAAGCTTGTATG+AGG - chr2_4:44002250-44002269 Msa0330000:intron 40.0%
AGCGGTGGTATACATGAAAT+AGG + chr2_4:44002363-44002382 None:intergenic 40.0%
GAGATGAACTTTCTCGATGA+CGG - chr2_4:44002388-44002407 Msa0330000:intron 40.0%
TGCTTCTCAAGCAACTTGAA+AGG - chr2_4:44002509-44002528 Msa0330000:intron 40.0%
! TGGTTTCAGCCCTTTTATAG+CGG - chr2_4:44002657-44002676 Msa0330000:intron 40.0%
AGGTGTAAAGACACAAGCAA+AGG + chr2_4:44002814-44002833 None:intergenic 40.0%
TTCCAAACCCTCACAATGTA+AGG - chr2_4:44003465-44003484 Msa0330000:intron 40.0%
TATACTTGACAAGTTAGCGG+AGG - chr2_4:44003508-44003527 Msa0330000:intron 40.0%
GCGAATAAGTGAGAAATTCG+TGG - chr2_4:44003964-44003983 Msa0330000:intron 40.0%
TAATGTTCCATTCATGGGTC+GGG + chr2_4:44004584-44004603 None:intergenic 40.0%
CTAATGTTCCATTCATGGGT+CGG + chr2_4:44004585-44004604 None:intergenic 40.0%
GACCCATGAATGGAACATTA+GGG - chr2_4:44004584-44004603 Msa0330000:intron 40.0%
AGCCCTAATGTTCCATTCAT+GGG + chr2_4:44004589-44004608 None:intergenic 40.0%
ATCATCCAACATGAGAGCTA+AGG - chr2_4:44004736-44004755 Msa0330000:intron 40.0%
CGTTCCAAATGATAGAACCA+TGG - chr2_4:44004993-44005012 Msa0330000:intron 40.0%
! CCAACCATGGTTCTATCATT+TGG + chr2_4:44005000-44005019 None:intergenic 40.0%
CCAAATGATAGAACCATGGT+TGG - chr2_4:44004997-44005016 Msa0330000:intron 40.0%
CAATCCCACAAGCATCATAA+TGG + chr2_4:44005453-44005472 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG - chr2_4:44005924-44005943 Msa0330000:intron 40.0%
ATCTAAGTAGGTGCATATGC+AGG - chr2_4:44005936-44005955 Msa0330000:intron 40.0%
AGGCTAACATATCATGAAGC+CGG + chr2_4:44005988-44006007 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG - chr2_4:44005992-44006011 Msa0330000:intron 40.0%
CAAGATCAATACACCAGAAC+AGG + chr2_4:44006008-44006027 None:intergenic 40.0%
! GTTCTGGTGTATTGATCTTG+AGG - chr2_4:44006008-44006027 Msa0330000:intron 40.0%
! GGTGTATTGATCTTGAGGTT+TGG - chr2_4:44006013-44006032 Msa0330000:intron 40.0%
ATCTTAAGCTAACCCAGTGA+AGG + chr2_4:44006273-44006292 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG - chr2_4:44006338-44006357 Msa0330000:CDS 40.0%
!! ATGGATTGGTGATGGTAGAG+AGG - chr2_4:44000721-44000740 Msa0330000:three_prime_UTR 45.0%
!! TGGATTGGTGATGGTAGAGA+GGG - chr2_4:44000722-44000741 Msa0330000:three_prime_UTR 45.0%
TTCTCCCTCTTCTCTCTAAG+TGG + chr2_4:44000756-44000775 None:intergenic 45.0%
TGTCAGAGGTTAGGTGTTCA+GGG + chr2_4:44000948-44000967 None:intergenic 45.0%
AATTGGACGTGTCAGAGGTT+AGG + chr2_4:44000957-44000976 None:intergenic 45.0%
TTAGCAATTGGACGTGTCAG+AGG + chr2_4:44000962-44000981 None:intergenic 45.0%
ACATGCCAGTCGTCAGATAT+AGG + chr2_4:44000989-44001008 None:intergenic 45.0%
GGGAACTTATTTAGAGGTGG+AGG + chr2_4:44001102-44001121 None:intergenic 45.0%
TGGATAAGTCATGGCATGCA+AGG + chr2_4:44001184-44001203 None:intergenic 45.0%
GGAATTTGTACCAACTCCCA+AGG - chr2_4:44001307-44001326 Msa0330000:intron 45.0%
! ACTTTTTGCCCTTGTTGCTC+AGG + chr2_4:44001396-44001415 None:intergenic 45.0%
! TAATACTTGCCTGATGGCGT+TGG + chr2_4:44001514-44001533 None:intergenic 45.0%
TCTCTCACAAAGCGACACAA+TGG - chr2_4:44001717-44001736 Msa0330000:intron 45.0%
TTAATGTTACCCCTCCGAAG+AGG - chr2_4:44001759-44001778 Msa0330000:intron 45.0%
TAATGTTACCCCTCCGAAGA+GGG - chr2_4:44001760-44001779 Msa0330000:intron 45.0%
! AATCTTTTCCCTCTTCGGAG+GGG + chr2_4:44001771-44001790 None:intergenic 45.0%
! GAATCTTTTCCCTCTTCGGA+GGG + chr2_4:44001772-44001791 None:intergenic 45.0%
! AGAATCTTTTCCCTCTTCGG+AGG + chr2_4:44001773-44001792 None:intergenic 45.0%
ATATGAGTGGGGAGAGAGTT+GGG + chr2_4:44002164-44002183 None:intergenic 45.0%
AGTTCATCTCAAAAGAGCGG+TGG + chr2_4:44002378-44002397 None:intergenic 45.0%
CACCTTACATTGTGAGGGTT+TGG + chr2_4:44003470-44003489 None:intergenic 45.0%
TCTCGCACCTTACATTGTGA+GGG + chr2_4:44003475-44003494 None:intergenic 45.0%
ATCTCGCACCTTACATTGTG+AGG + chr2_4:44003476-44003495 None:intergenic 45.0%
!!! TTTTTTTCTTCTGGCGCCAC+TGG - chr2_4:44003791-44003810 Msa0330000:intron 45.0%
CGACCCATGAATGGAACATT+AGG - chr2_4:44004583-44004602 Msa0330000:intron 45.0%
GAGCCCTAATGTTCCATTCA+TGG + chr2_4:44004590-44004609 None:intergenic 45.0%
TGAACGATGGCAAATTTCCG+CGG + chr2_4:44004692-44004711 None:intergenic 45.0%
TCTCAAGCTTCTCTGAACGA+TGG + chr2_4:44004705-44004724 None:intergenic 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG - chr2_4:44005593-44005612 Msa0330000:intron 45.0%
!!! TTTTTTCCTGTCGTGGCTGT+TGG - chr2_4:44005802-44005821 Msa0330000:intron 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG - chr2_4:44005878-44005897 Msa0330000:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG - chr2_4:44005966-44005985 Msa0330000:intron 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG - chr2_4:44006022-44006041 Msa0330000:intron 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG - chr2_4:44006114-44006133 Msa0330000:intron 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG - chr2_4:44006274-44006293 Msa0330000:CDS 45.0%
!! AAAATTATTTATTACATATT+AGG - chr2_4:44002532-44002551 Msa0330000:intron 5.0%
!! ATTTAAAAAAGAAAAAAAAT+TGG + chr2_4:44003841-44003860 None:intergenic 5.0%
!!! AAAAATATTTTTACAATTTT+TGG - chr2_4:44005274-44005293 Msa0330000:intron 5.0%
!! TGGTGATGGTAGAGAGGGAA+AGG - chr2_4:44000727-44000746 Msa0330000:three_prime_UTR 50.0%
! GGTGATGGTAGAGAGGGAAA+GGG - chr2_4:44000728-44000747 Msa0330000:three_prime_UTR 50.0%
GTGTCAGAGGTTAGGTGTTC+AGG + chr2_4:44000949-44000968 None:intergenic 50.0%
TGTTGGACTTCCTTGGGAGT+TGG + chr2_4:44001320-44001339 None:intergenic 50.0%
TCGGATACACCTGAGCAACA+AGG - chr2_4:44001384-44001403 Msa0330000:intron 50.0%
CGGATACACCTGAGCAACAA+GGG - chr2_4:44001385-44001404 Msa0330000:intron 50.0%
AAAGAAGAGAGGGAAGGCTC+TGG + chr2_4:44002072-44002091 None:intergenic 50.0%
GTTGGGGAAAGGCAATGAAG+AGG + chr2_4:44002147-44002166 None:intergenic 50.0%
TATGAGTGGGGAGAGAGTTG+GGG + chr2_4:44002163-44002182 None:intergenic 50.0%
GATATGAGTGGGGAGAGAGT+TGG + chr2_4:44002165-44002184 None:intergenic 50.0%
GTGCAGCGTTTCGTAAGAAG+CGG + chr2_4:44004612-44004631 None:intergenic 50.0%
ATAAAGCCAACAGCCACGAC+AGG + chr2_4:44005811-44005830 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG - chr2_4:44005881-44005900 Msa0330000:intron 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG - chr2_4:44006021-44006040 Msa0330000:intron 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG - chr2_4:44006023-44006042 Msa0330000:intron 50.0%
GGCGCCACTTAGAGAGAAGA+GGG - chr2_4:44000749-44000768 Msa0330000:three_prime_UTR 55.0%
CGACTGGCATGTTACACACG+CGG - chr2_4:44000997-44001016 Msa0330000:three_prime_UTR 55.0%
TGGTCCTTTCCAACGCCATC+AGG - chr2_4:44001502-44001521 Msa0330000:intron 55.0%
! CTTGCCTGATGGCGTTGGAA+AGG + chr2_4:44001509-44001528 None:intergenic 55.0%
! GCTTGGGAGTGCAATCCGAA+TGG + chr2_4:44001588-44001607 None:intergenic 55.0%
TTGTCGACCCCAATCCGTGA+GGG + chr2_4:44003358-44003377 None:intergenic 55.0%
ATTGTCGACCCCAATCCGTG+AGG + chr2_4:44003359-44003378 None:intergenic 55.0%
GTTCCATTCATGGGTCGGGT+CGG + chr2_4:44004580-44004599 None:intergenic 55.0%
CAAATTTCCGCGGCAGGGAA+GGG + chr2_4:44004682-44004701 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG + chr2_4:44004687-44004706 None:intergenic 55.0%
AGCGTATGAGGCGGTTGAAG+AGG - chr2_4:44005890-44005909 Msa0330000:intron 55.0%
GAAGCCGGCATCAAGCAAGA+TGG + chr2_4:44005973-44005992 None:intergenic 55.0%
GGGCGCCACTTAGAGAGAAG+AGG - chr2_4:44000748-44000767 Msa0330000:three_prime_UTR 60.0%
GGCATGTTACACACGCGGAG+TGG - chr2_4:44001002-44001021 Msa0330000:three_prime_UTR 60.0%
GTGGGGAGAGAGTTGGGGAA+AGG + chr2_4:44002158-44002177 None:intergenic 60.0%
GACAGTGGCAGAGTCCCTCA+CGG - chr2_4:44003341-44003360 Msa0330000:intron 60.0%
TGGCAGAGTCCCTCACGGAT+TGG - chr2_4:44003346-44003365 Msa0330000:intron 60.0%
GGCAGAGTCCCTCACGGATT+GGG - chr2_4:44003347-44003366 Msa0330000:intron 60.0%
! GCAGAGTCCCTCACGGATTG+GGG - chr2_4:44003348-44003367 Msa0330000:intron 60.0%
CAACCGACCCGACCCATGAA+TGG - chr2_4:44004574-44004593 Msa0330000:intron 60.0%
TTCAGTTCCCTTCCCTGCCG+CGG - chr2_4:44004672-44004691 Msa0330000:intron 60.0%
GCAAATTTCCGCGGCAGGGA+AGG + chr2_4:44004683-44004702 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG + chr2_4:44004688-44004707 None:intergenic 60.0%
GGGGTCGACAATGACCCACC+CGG - chr2_4:44003367-44003386 Msa0330000:intron 65.0%
!! CTTCTGGCGCCACTGGTTCC+TGG - chr2_4:44003798-44003817 Msa0330000:intron 65.0%
GTCGGGTCGGTTGTGTACCC+GGG + chr2_4:44004567-44004586 None:intergenic 65.0%
GGTCGGGTCGGTTGTGTACC+CGG + chr2_4:44004568-44004587 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 44000629 44006379 44000629 ID=Msa0330000;Name=Msa0330000
chr2_4 mRNA 44000629 44006379 44000629 ID=Msa0330000-mRNA-1;Parent=Msa0330000;Name=Msa0330000-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0.5|0.5|1|1|2|438|55
chr2_4 exon 44000629 44001128 44000629 ID=Msa0330000-mRNA-1:exon:13432;Parent=Msa0330000-mRNA-1
chr2_4 exon 44006274 44006379 44006274 ID=Msa0330000-mRNA-1:exon:13431;Parent=Msa0330000-mRNA-1
chr2_4 CDS 44006274 44006379 44006274 ID=Msa0330000-mRNA-1:cds;Parent=Msa0330000-mRNA-1
chr2_4 CDS 44001067 44001128 44001067 ID=Msa0330000-mRNA-1:cds;Parent=Msa0330000-mRNA-1
chr2_4 three_prime_UTR 44000629 44001066 44000629 ID=Msa0330000-mRNA-1:three_prime_utr;Parent=Msa0330000-mRNA-1
Gene Sequence

>Msa0330000

ATGGAGCCAATGCTTACTTCTTGCTATCAATCTCTTCTAAAAGCAGGATTAGTGCCATCACAAACCATGATTCTTGAAGGAAGTAGCTTGAAATGGATTGGTGATGTGGAAGAAGAAGCGTATGAGGCGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA
Protein sequence

>Msa0330000

MEPMLTSCYQSLLKAGLVPSQTMILEGSSLKWIGDVEEEAYEAVEEEAPKDETEI*