AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0965480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0965480 MtrunA17_Chr6g0486051 90.123 162 0 1 1 162 1 146 3.32e-100 284
Msa0965480 MtrunA17_Chr7g0265731 55.828 163 70 1 2 162 55 217 3.36e-63 193
Msa0965480 MtrunA17_Chr2g0287981 42.446 139 79 1 22 159 58 196 4.58e-33 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0965480 AT1G65980.1 73.457 162 43 0 1 162 1 162 4.08e-92 265
Msa0965480 AT1G65970.1 72.222 162 45 0 1 162 1 162 2.04e-90 260
Msa0965480 AT1G60740.1 71.605 162 46 0 1 162 1 162 2.07e-89 258
Msa0965480 AT3G52960.1 56.098 164 69 2 2 162 71 234 4.15e-61 189
Msa0965480 AT1G65990.1 58.553 152 59 1 1 152 1 148 3.87e-59 192
Msa0965480 AT1G65980.2 73.077 78 21 0 1 78 1 78 1.51e-39 129
Msa0965480 AT3G06050.1 46.032 126 67 1 35 159 73 198 1.67e-35 122
Msa0965480 AT3G06050.2 53.571 56 26 0 35 90 73 128 1.54e-18 78.6

Find 63 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCCTTATATAGGGAAAT+TGG 0.205101 6_3:+92870886 None:intergenic
CCCAAGAGCATGTGTGTATT+TGG 0.245539 6_3:+92869064 None:intergenic
ATTTCCAATTTCCCTATATA+AGG 0.268902 6_3:-92870890 Msa0965480:five_prime_UTR
GTCATCATCTTTGCTGTTCC+TGG 0.271985 6_3:-92870663 Msa0965480:CDS
CATTTGCAACCTTCACCTTC+AGG 0.298861 6_3:+92868973 None:intergenic
ATGTTTCAATTATGATGTTC+TGG 0.299613 6_3:+92868860 None:intergenic
TGTAATGACCCTCCTTATAT+AGG 0.303639 6_3:+92870878 None:intergenic
ACATGATGTAATGCATATTA+TGG 0.320264 6_3:-92868805 Msa0965480:three_prime_UTR
AACAGCTTGAAGCATGTTCC+TGG 0.350575 6_3:-92869860 Msa0965480:intron
AACTTGACATGCTTGTTCTC+TGG 0.352143 6_3:+92869104 None:intergenic
ATCACATCTCCTACTTTGAT+TGG 0.352692 6_3:+92870768 None:intergenic
GCCAAATACACACATGCTCT+TGG 0.365981 6_3:-92869065 Msa0965480:CDS
GTAATGACCCTCCTTATATA+GGG 0.383733 6_3:+92870879 None:intergenic
AGCCATTGAAGGTGACTGTT+AGG 0.385099 6_3:+92870791 None:intergenic
AGGAAGGCTAAGGTGCCATC+TGG 0.393148 6_3:+92870744 None:intergenic
ATCCATTTGTGCTGAATTCA+TGG 0.400960 6_3:-92869139 Msa0965480:CDS
GCCCATGAATTCAGCACAAA+TGG 0.408653 6_3:+92869137 None:intergenic
ACTTGACATGCTTGTTCTCT+GGG 0.413856 6_3:+92869105 None:intergenic
AAGGTGACTGTTAGGGTGAT+TGG 0.414976 6_3:+92870799 None:intergenic
CTTGTTCTCTGGGAATGTCT+TGG 0.426261 6_3:+92869115 None:intergenic
TTATATAGGGAAATTGGAAA+TGG 0.426387 6_3:+92870892 None:intergenic
GAATGAATGGTGACAGATTG+AGG 0.441168 6_3:+92870705 None:intergenic
AACAAGAAGAGCAAACCTCT+TGG 0.455187 6_3:+92869001 None:intergenic
ACTTTGATTGGAGCCATTGA+AGG 0.462425 6_3:+92870780 None:intergenic
GTGATTGGTGAGAGTGTTGT+TGG 0.463994 6_3:+92870814 None:intergenic
CCAAATACACACATGCTCTT+GGG 0.477554 6_3:-92869064 Msa0965480:CDS
ACCCTAACAGTCACCTTCAA+TGG 0.490966 6_3:-92870793 Msa0965480:exon
CTTGATGATTTCTTCAGCAC+CGG 0.493646 6_3:+92868923 None:intergenic
GCCGGCAGAGAGGGAATGAA+TGG 0.496618 6_3:+92870692 None:intergenic
TCCAATTTCCCTATATAAGG+AGG 0.506911 6_3:-92870887 Msa0965480:five_prime_UTR
TTCTTCATCAAGGAAGGCTA+AGG 0.511511 6_3:+92870734 None:intergenic
GACCTCAGCGACAAAGGTCT+CGG 0.528430 6_3:-92869032 Msa0965480:CDS
GGAGGAGAATACACCATCTC+CGG 0.528627 6_3:-92868942 Msa0965480:CDS
TCAGAAGAGCTGAAAGGAAA+GGG 0.536940 6_3:-92869824 Msa0965480:CDS
GATTTCTTCAGCACCGGAGA+TGG 0.540136 6_3:+92868929 None:intergenic
TCTGATCTCTCAATGAAGCC+AGG 0.542391 6_3:+92869842 None:intergenic
CATGTCAAGTTCCTTGCTGA+TGG 0.542780 6_3:-92869092 Msa0965480:CDS
GGAAGGCTAAGGTGCCATCT+GGG 0.546906 6_3:+92870745 None:intergenic
GAGCTTGACCTCAGCGACAA+AGG 0.549694 6_3:-92869038 Msa0965480:CDS
CATGTGGGTGTGAAGGCACC+AGG 0.555852 6_3:+92870645 None:intergenic
ATACACACATGCTCTTGGGT+TGG 0.558587 6_3:-92869060 Msa0965480:CDS
ACCATTCATTCCCTCTCTGC+CGG 0.561297 6_3:-92870693 Msa0965480:CDS
AAGGTTGCAAATGTTGAAGA+AGG 0.566267 6_3:-92868963 Msa0965480:CDS
TCAATGGCTCCAATCAAAGT+AGG 0.568343 6_3:-92870777 Msa0965480:CDS
GCCATTGAAGGTGACTGTTA+GGG 0.568344 6_3:+92870792 None:intergenic
TCTTCTTGTTGAAGACCTGA+AGG 0.568574 6_3:-92868988 Msa0965480:CDS
CCAATTTCCCTATATAAGGA+GGG 0.570594 6_3:-92870886 Msa0965480:five_prime_UTR
ATCAGAAGAGCTGAAAGGAA+AGG 0.571012 6_3:-92869825 Msa0965480:CDS
GTGCCTTCACACCCACATGC+AGG 0.577377 6_3:-92870641 Msa0965480:intron
GTTGCAAATGTTGAAGAAGG+AGG 0.581974 6_3:-92868960 Msa0965480:CDS
TGCCGAGACCTTTGTCGCTG+AGG 0.592538 6_3:+92869030 None:intergenic
CGGTGCTGAAGAAATCATCA+AGG 0.594261 6_3:-92868922 Msa0965480:CDS
TCCATTTGTGCTGAATTCAT+GGG 0.611814 6_3:-92869138 Msa0965480:CDS
GAGAGATCAGAAGAGCTGAA+AGG 0.621848 6_3:-92869830 Msa0965480:CDS
GTAGGAGATGTGATCCCAGA+TGG 0.630920 6_3:-92870759 Msa0965480:CDS
TGTTGAAGACCTGAAGGTGA+AGG 0.633342 6_3:-92868982 Msa0965480:CDS
TAACCTGCATGTGGGTGTGA+AGG 0.642321 6_3:+92870638 None:intergenic
GTCTCGGCATTCGCTCCAAG+AGG 0.644758 6_3:-92869016 Msa0965480:CDS
AAAAGAGGTTAACCTGCATG+TGG 0.650855 6_3:+92870629 None:intergenic
GATGAAATCATCTGCATCAG+TGG 0.655212 6_3:-92869797 Msa0965480:intron
ATTTGGCAGAGCCATCAGCA+AGG 0.708029 6_3:+92869081 None:intergenic
AATGTCGCGTACTTTAGACA+AGG 0.726123 6_3:-92868828 Msa0965480:three_prime_UTR
AAAGAGGTTAACCTGCATGT+GGG 0.728425 6_3:+92870630 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATATTTTATTTCATTTTT+AGG + chr6_3:92869059-92869078 None:intergenic 10.0%
!! AAATAAAAAAGAAAACTTTA+GGG + chr6_3:92869120-92869139 None:intergenic 10.0%
!!! TTTTTCTTTTGTATAAAATT+CGG - chr6_3:92869604-92869623 Msa0965480:intron 10.0%
!!! CAATATTTTATTTCATTTTT+AGG + chr6_3:92869059-92869078 None:intergenic 10.0%
!! AAATAAAAAAGAAAACTTTA+GGG + chr6_3:92869120-92869139 None:intergenic 10.0%
!!! TTTTTCTTTTGTATAAAATT+CGG - chr6_3:92869604-92869623 Msa0965480:intron 10.0%
!! CAAATAAAAAAGAAAACTTT+AGG + chr6_3:92869121-92869140 None:intergenic 15.0%
!!! TTTTTATTTGATTTATGTGT+TGG - chr6_3:92869131-92869150 Msa0965480:CDS 15.0%
!!! TCAATTTGTCTTATTTTTTT+TGG - chr6_3:92869570-92869589 Msa0965480:intron 15.0%
!!! TATAAAATTCGGAAATTTTA+AGG - chr6_3:92869615-92869634 Msa0965480:intron 15.0%
!!! TCTAATTATGTTATTTTGTT+TGG - chr6_3:92869754-92869773 Msa0965480:intron 15.0%
!!! TGAATGTATTTTTTATAATG+TGG - chr6_3:92870141-92870160 Msa0965480:intron 15.0%
!! CAAATAAAAAAGAAAACTTT+AGG + chr6_3:92869121-92869140 None:intergenic 15.0%
!!! TTTTTATTTGATTTATGTGT+TGG - chr6_3:92869131-92869150 Msa0965480:CDS 15.0%
!!! TCAATTTGTCTTATTTTTTT+TGG - chr6_3:92869570-92869589 Msa0965480:intron 15.0%
!!! TATAAAATTCGGAAATTTTA+AGG - chr6_3:92869615-92869634 Msa0965480:intron 15.0%
!!! TCTAATTATGTTATTTTGTT+TGG - chr6_3:92869754-92869773 Msa0965480:intron 15.0%
!!! TGAATGTATTTTTTATAATG+TGG - chr6_3:92870141-92870160 Msa0965480:intron 15.0%
!! AAAATATTGAATCAGATTCA+TGG - chr6_3:92869070-92869089 Msa0965480:CDS 20.0%
!! AAATATTGAATCAGATTCAT+GGG - chr6_3:92869071-92869090 Msa0965480:CDS 20.0%
!! ATGCAAAAAAAATGAATCAA+AGG + chr6_3:92869181-92869200 None:intergenic 20.0%
!! GAGAAAAACAAATAAATCAA+AGG + chr6_3:92869262-92869281 None:intergenic 20.0%
!! GTGAAAAACAAATAAATCAA+AGG + chr6_3:92869344-92869363 None:intergenic 20.0%
!! GTGAAAAACAAATAAATCAA+AGG + chr6_3:92869344-92869363 None:intergenic 20.0%
!! TAAAGACAACCTTATAAAAA+GGG + chr6_3:92869695-92869714 None:intergenic 20.0%
!! AAAATATTGAATCAGATTCA+TGG - chr6_3:92869070-92869089 Msa0965480:CDS 20.0%
!! AAATATTGAATCAGATTCAT+GGG - chr6_3:92869071-92869090 Msa0965480:CDS 20.0%
!! ATGCAAAAAAAATGAATCAA+AGG + chr6_3:92869181-92869200 None:intergenic 20.0%
!! GAGAAAAACAAATAAATCAA+AGG + chr6_3:92869262-92869281 None:intergenic 20.0%
!! GTGAAAAACAAATAAATCAA+AGG + chr6_3:92869344-92869363 None:intergenic 20.0%
!! GTGAAAAACAAATAAATCAA+AGG + chr6_3:92869344-92869363 None:intergenic 20.0%
!! TAAAGACAACCTTATAAAAA+GGG + chr6_3:92869695-92869714 None:intergenic 20.0%
! AAGAATGTAACCAAAAAAAG+AGG - chr6_3:92868717-92868736 Msa0965480:three_prime_UTR 25.0%
! TTATATAGGGAAATTGGAAA+TGG + chr6_3:92868748-92868767 None:intergenic 25.0%
! ATTTCCAATTTCCCTATATA+AGG - chr6_3:92868747-92868766 Msa0965480:three_prime_UTR 25.0%
! GTTAAGATTTGAAGGAAAAT+TGG + chr6_3:92869036-92869055 None:intergenic 25.0%
!!! TTTTTAGGGTTAAGATTTGA+AGG + chr6_3:92869044-92869063 None:intergenic 25.0%
!! ATTTAACTGAATTAGCTTCA+TGG - chr6_3:92869233-92869252 Msa0965480:intron 25.0%
!! TTTAACTGAATTAGCTTCAT+GGG - chr6_3:92869234-92869253 Msa0965480:intron 25.0%
!!! ATTAGACTGAATTAGCTTTA+TGG - chr6_3:92869315-92869334 Msa0965480:intron 25.0%
!!! TTAGACTGAATTAGCTTTAT+GGG - chr6_3:92869316-92869335 Msa0965480:intron 25.0%
!!! ATCTGTGAGAATTTTGAAAT+GGG - chr6_3:92869543-92869562 Msa0965480:intron 25.0%
!!! AATTTTAAGGATACTGTTTG+AGG - chr6_3:92869628-92869647 Msa0965480:intron 25.0%
!!! GTTTTGTTTCCCTTTTTATA+AGG - chr6_3:92869683-92869702 Msa0965480:intron 25.0%
! GTAAAGACAACCTTATAAAA+AGG + chr6_3:92869696-92869715 None:intergenic 25.0%
! ATAAGGTTGTCTTTACAAAA+CGG - chr6_3:92869700-92869719 Msa0965480:intron 25.0%
! ATCTATGAATGCTTCAAAAT+TGG - chr6_3:92869994-92870013 Msa0965480:intron 25.0%
! TCTATGAATGCTTCAAAATT+GGG - chr6_3:92869995-92870014 Msa0965480:intron 25.0%
! AATTACAAAGCTTGAAATCT+AGG + chr6_3:92870282-92870301 None:intergenic 25.0%
! ATGTTTCAATTATGATGTTC+TGG + chr6_3:92870780-92870799 None:intergenic 25.0%
! ACATGATGTAATGCATATTA+TGG - chr6_3:92870832-92870851 Msa0965480:five_prime_UTR 25.0%
!!! AGTCAGTTTTTCAATTGTAA+TGG - chr6_3:92870909-92870928 Msa0965480:five_prime_UTR 25.0%
! AAGAATGTAACCAAAAAAAG+AGG - chr6_3:92868717-92868736 Msa0965480:three_prime_UTR 25.0%
! TTATATAGGGAAATTGGAAA+TGG + chr6_3:92868748-92868767 None:intergenic 25.0%
! ATTTCCAATTTCCCTATATA+AGG - chr6_3:92868747-92868766 Msa0965480:three_prime_UTR 25.0%
! GTTAAGATTTGAAGGAAAAT+TGG + chr6_3:92869036-92869055 None:intergenic 25.0%
!!! TTTTTAGGGTTAAGATTTGA+AGG + chr6_3:92869044-92869063 None:intergenic 25.0%
!! ATTTAACTGAATTAGCTTCA+TGG - chr6_3:92869233-92869252 Msa0965480:intron 25.0%
!! TTTAACTGAATTAGCTTCAT+GGG - chr6_3:92869234-92869253 Msa0965480:intron 25.0%
!!! ATTAGACTGAATTAGCTTTA+TGG - chr6_3:92869315-92869334 Msa0965480:intron 25.0%
!!! TTAGACTGAATTAGCTTTAT+GGG - chr6_3:92869316-92869335 Msa0965480:intron 25.0%
!!! ATCTGTGAGAATTTTGAAAT+GGG - chr6_3:92869543-92869562 Msa0965480:intron 25.0%
!!! AATTTTAAGGATACTGTTTG+AGG - chr6_3:92869628-92869647 Msa0965480:intron 25.0%
!!! GTTTTGTTTCCCTTTTTATA+AGG - chr6_3:92869683-92869702 Msa0965480:intron 25.0%
! GTAAAGACAACCTTATAAAA+AGG + chr6_3:92869696-92869715 None:intergenic 25.0%
! ATAAGGTTGTCTTTACAAAA+CGG - chr6_3:92869700-92869719 Msa0965480:intron 25.0%
! ATCTATGAATGCTTCAAAAT+TGG - chr6_3:92869994-92870013 Msa0965480:intron 25.0%
! TCTATGAATGCTTCAAAATT+GGG - chr6_3:92869995-92870014 Msa0965480:intron 25.0%
! AATTACAAAGCTTGAAATCT+AGG + chr6_3:92870282-92870301 None:intergenic 25.0%
! ATGTTTCAATTATGATGTTC+TGG + chr6_3:92870780-92870799 None:intergenic 25.0%
! ACATGATGTAATGCATATTA+TGG - chr6_3:92870832-92870851 Msa0965480:five_prime_UTR 25.0%
!!! AGTCAGTTTTTCAATTGTAA+TGG - chr6_3:92870909-92870928 Msa0965480:five_prime_UTR 25.0%
!!! AATGGATGATCCTCTTTTTT+TGG + chr6_3:92868730-92868749 None:intergenic 30.0%
!! TTTGTTTTCTTCATCAAGGA+AGG + chr6_3:92868912-92868931 None:intergenic 30.0%
!!! AAAGATGATGACTTTTTTGC+CGG + chr6_3:92868966-92868985 None:intergenic 30.0%
! ATTAGACTGAATTAGCTTCA+TGG - chr6_3:92869397-92869416 Msa0965480:intron 30.0%
! TTAGACTGAATTAGCTTCAT+GGG - chr6_3:92869398-92869417 Msa0965480:intron 30.0%
!! GATCTGTGAGAATTTTGAAA+TGG - chr6_3:92869542-92869561 Msa0965480:intron 30.0%
TAAGGTTGTCTTTACAAAAC+GGG - chr6_3:92869701-92869720 Msa0965480:intron 30.0%
CTATGAATGCTTCAAAATTG+GGG - chr6_3:92869996-92870015 Msa0965480:intron 30.0%
! GCTTGTTACTTGATTCTATA+TGG - chr6_3:92870018-92870037 Msa0965480:intron 30.0%
TGATTCAATGTCTTGCTAAA+TGG - chr6_3:92870100-92870119 Msa0965480:intron 30.0%
AGAAAAGCTGCATTAAAATC+TGG - chr6_3:92870318-92870337 Msa0965480:intron 30.0%
! TTACTAATGTTACCGATTAC+AGG - chr6_3:92870346-92870365 Msa0965480:intron 30.0%
AAATTCAGATTGCCTGTAAT+CGG + chr6_3:92870361-92870380 None:intergenic 30.0%
CAACATCATAAGTATAGAAC+AGG + chr6_3:92870405-92870424 None:intergenic 30.0%
CTGTTCTATACTTATGATGT+TGG - chr6_3:92870403-92870422 Msa0965480:intron 30.0%
!!! AATGGATGATCCTCTTTTTT+TGG + chr6_3:92868730-92868749 None:intergenic 30.0%
!! TTTGTTTTCTTCATCAAGGA+AGG + chr6_3:92868912-92868931 None:intergenic 30.0%
!!! AAAGATGATGACTTTTTTGC+CGG + chr6_3:92868966-92868985 None:intergenic 30.0%
! ATTAGACTGAATTAGCTTCA+TGG - chr6_3:92869397-92869416 Msa0965480:intron 30.0%
! TTAGACTGAATTAGCTTCAT+GGG - chr6_3:92869398-92869417 Msa0965480:intron 30.0%
!! GATCTGTGAGAATTTTGAAA+TGG - chr6_3:92869542-92869561 Msa0965480:intron 30.0%
TAAGGTTGTCTTTACAAAAC+GGG - chr6_3:92869701-92869720 Msa0965480:intron 30.0%
CTATGAATGCTTCAAAATTG+GGG - chr6_3:92869996-92870015 Msa0965480:intron 30.0%
! GCTTGTTACTTGATTCTATA+TGG - chr6_3:92870018-92870037 Msa0965480:intron 30.0%
TGATTCAATGTCTTGCTAAA+TGG - chr6_3:92870100-92870119 Msa0965480:intron 30.0%
AGAAAAGCTGCATTAAAATC+TGG - chr6_3:92870318-92870337 Msa0965480:intron 30.0%
! TTACTAATGTTACCGATTAC+AGG - chr6_3:92870346-92870365 Msa0965480:intron 30.0%
AAATTCAGATTGCCTGTAAT+CGG + chr6_3:92870361-92870380 None:intergenic 30.0%
CAACATCATAAGTATAGAAC+AGG + chr6_3:92870405-92870424 None:intergenic 30.0%
CTGTTCTATACTTATGATGT+TGG - chr6_3:92870403-92870422 Msa0965480:intron 30.0%
TCCAATTTCCCTATATAAGG+AGG - chr6_3:92868750-92868769 Msa0965480:three_prime_UTR 35.0%
CCAATTTCCCTATATAAGGA+GGG - chr6_3:92868751-92868770 Msa0965480:three_prime_UTR 35.0%
GTAATGACCCTCCTTATATA+GGG + chr6_3:92868761-92868780 None:intergenic 35.0%
TGTAATGACCCTCCTTATAT+AGG + chr6_3:92868762-92868781 None:intergenic 35.0%
ATCACATCTCCTACTTTGAT+TGG + chr6_3:92868872-92868891 None:intergenic 35.0%
!! GAGGTTTGTTTTCTTCATCA+AGG + chr6_3:92868916-92868935 None:intergenic 35.0%
GAAGGAAAATTGGTGAAAAG+AGG + chr6_3:92869026-92869045 None:intergenic 35.0%
!! ATTTCACTCGATTAGCTTCA+TGG - chr6_3:92869152-92869171 Msa0965480:intron 35.0%
!! TTTCACTCGATTAGCTTCAT+GGG - chr6_3:92869153-92869172 Msa0965480:intron 35.0%
AAGGTTGTCTTTACAAAACG+GGG - chr6_3:92869702-92869721 Msa0965480:intron 35.0%
TGTCTTGCTAAATGGTGATA+TGG - chr6_3:92870108-92870127 Msa0965480:intron 35.0%
TAACACATTGTATTCGCCAA+TGG + chr6_3:92870187-92870206 None:intergenic 35.0%
ATCCATTTGTGCTGAATTCA+TGG - chr6_3:92870498-92870517 Msa0965480:intron 35.0%
TCCATTTGTGCTGAATTCAT+GGG - chr6_3:92870499-92870518 Msa0965480:intron 35.0%
AAGGTTGCAAATGTTGAAGA+AGG - chr6_3:92870674-92870693 Msa0965480:CDS 35.0%
TCCAATTTCCCTATATAAGG+AGG - chr6_3:92868750-92868769 Msa0965480:three_prime_UTR 35.0%
CCAATTTCCCTATATAAGGA+GGG - chr6_3:92868751-92868770 Msa0965480:three_prime_UTR 35.0%
GTAATGACCCTCCTTATATA+GGG + chr6_3:92868761-92868780 None:intergenic 35.0%
TGTAATGACCCTCCTTATAT+AGG + chr6_3:92868762-92868781 None:intergenic 35.0%
ATCACATCTCCTACTTTGAT+TGG + chr6_3:92868872-92868891 None:intergenic 35.0%
!! GAGGTTTGTTTTCTTCATCA+AGG + chr6_3:92868916-92868935 None:intergenic 35.0%
GAAGGAAAATTGGTGAAAAG+AGG + chr6_3:92869026-92869045 None:intergenic 35.0%
!! ATTTCACTCGATTAGCTTCA+TGG - chr6_3:92869152-92869171 Msa0965480:intron 35.0%
!! TTTCACTCGATTAGCTTCAT+GGG - chr6_3:92869153-92869172 Msa0965480:intron 35.0%
AAGGTTGTCTTTACAAAACG+GGG - chr6_3:92869702-92869721 Msa0965480:intron 35.0%
TGTCTTGCTAAATGGTGATA+TGG - chr6_3:92870108-92870127 Msa0965480:intron 35.0%
TAACACATTGTATTCGCCAA+TGG + chr6_3:92870187-92870206 None:intergenic 35.0%
ATCCATTTGTGCTGAATTCA+TGG - chr6_3:92870498-92870517 Msa0965480:intron 35.0%
TCCATTTGTGCTGAATTCAT+GGG - chr6_3:92870499-92870518 Msa0965480:intron 35.0%
AAGGTTGCAAATGTTGAAGA+AGG - chr6_3:92870674-92870693 Msa0965480:CDS 35.0%
CCCTCCTTATATAGGGAAAT+TGG + chr6_3:92868754-92868773 None:intergenic 40.0%
ACTTTGATTGGAGCCATTGA+AGG + chr6_3:92868860-92868879 None:intergenic 40.0%
TCAATGGCTCCAATCAAAGT+AGG - chr6_3:92868860-92868879 Msa0965480:three_prime_UTR 40.0%
TTCTTCATCAAGGAAGGCTA+AGG + chr6_3:92868906-92868925 None:intergenic 40.0%
GAATGAATGGTGACAGATTG+AGG + chr6_3:92868935-92868954 None:intergenic 40.0%
AAAGAGGTTAACCTGCATGT+GGG + chr6_3:92869010-92869029 None:intergenic 40.0%
AAAAGAGGTTAACCTGCATG+TGG + chr6_3:92869011-92869030 None:intergenic 40.0%
AGGATACTGTTTGAGGATAG+AGG - chr6_3:92869635-92869654 Msa0965480:intron 40.0%
TTGAGGATAGAGGAACATCA+TGG - chr6_3:92869645-92869664 Msa0965480:intron 40.0%
ATCAGAAGAGCTGAAAGGAA+AGG - chr6_3:92869812-92869831 Msa0965480:CDS 40.0%
TCAGAAGAGCTGAAAGGAAA+GGG - chr6_3:92869813-92869832 Msa0965480:CDS 40.0%
GATGAAATCATCTGCATCAG+TGG - chr6_3:92869840-92869859 Msa0965480:CDS 40.0%
! AACTGAAAGCTTTGTGCCAT+TGG - chr6_3:92870168-92870187 Msa0965480:intron 40.0%
TTTGTCACATCGATACCTGT+TGG - chr6_3:92870232-92870251 Msa0965480:intron 40.0%
GCTTGAAATCTAGGCAATCT+AGG + chr6_3:92870273-92870292 None:intergenic 40.0%
ACTTGACATGCTTGTTCTCT+GGG + chr6_3:92870535-92870554 None:intergenic 40.0%
AACTTGACATGCTTGTTCTC+TGG + chr6_3:92870536-92870555 None:intergenic 40.0%
CCAAATACACACATGCTCTT+GGG - chr6_3:92870573-92870592 Msa0965480:intron 40.0%
AACAAGAAGAGCAAACCTCT+TGG + chr6_3:92870639-92870658 None:intergenic 40.0%
TCTTCTTGTTGAAGACCTGA+AGG - chr6_3:92870649-92870668 Msa0965480:CDS 40.0%
GTTGCAAATGTTGAAGAAGG+AGG - chr6_3:92870677-92870696 Msa0965480:CDS 40.0%
! CTTGATGATTTCTTCAGCAC+CGG + chr6_3:92870717-92870736 None:intergenic 40.0%
AATGTCGCGTACTTTAGACA+AGG - chr6_3:92870809-92870828 Msa0965480:five_prime_UTR 40.0%
CCCTCCTTATATAGGGAAAT+TGG + chr6_3:92868754-92868773 None:intergenic 40.0%
ACTTTGATTGGAGCCATTGA+AGG + chr6_3:92868860-92868879 None:intergenic 40.0%
TCAATGGCTCCAATCAAAGT+AGG - chr6_3:92868860-92868879 Msa0965480:three_prime_UTR 40.0%
TTCTTCATCAAGGAAGGCTA+AGG + chr6_3:92868906-92868925 None:intergenic 40.0%
GAATGAATGGTGACAGATTG+AGG + chr6_3:92868935-92868954 None:intergenic 40.0%
AAAGAGGTTAACCTGCATGT+GGG + chr6_3:92869010-92869029 None:intergenic 40.0%
AAAAGAGGTTAACCTGCATG+TGG + chr6_3:92869011-92869030 None:intergenic 40.0%
AGGATACTGTTTGAGGATAG+AGG - chr6_3:92869635-92869654 Msa0965480:intron 40.0%
TTGAGGATAGAGGAACATCA+TGG - chr6_3:92869645-92869664 Msa0965480:intron 40.0%
ATCAGAAGAGCTGAAAGGAA+AGG - chr6_3:92869812-92869831 Msa0965480:CDS 40.0%
TCAGAAGAGCTGAAAGGAAA+GGG - chr6_3:92869813-92869832 Msa0965480:CDS 40.0%
GATGAAATCATCTGCATCAG+TGG - chr6_3:92869840-92869859 Msa0965480:CDS 40.0%
! AACTGAAAGCTTTGTGCCAT+TGG - chr6_3:92870168-92870187 Msa0965480:intron 40.0%
TTTGTCACATCGATACCTGT+TGG - chr6_3:92870232-92870251 Msa0965480:intron 40.0%
GCTTGAAATCTAGGCAATCT+AGG + chr6_3:92870273-92870292 None:intergenic 40.0%
ACTTGACATGCTTGTTCTCT+GGG + chr6_3:92870535-92870554 None:intergenic 40.0%
AACTTGACATGCTTGTTCTC+TGG + chr6_3:92870536-92870555 None:intergenic 40.0%
CCAAATACACACATGCTCTT+GGG - chr6_3:92870573-92870592 Msa0965480:intron 40.0%
AACAAGAAGAGCAAACCTCT+TGG + chr6_3:92870639-92870658 None:intergenic 40.0%
TCTTCTTGTTGAAGACCTGA+AGG - chr6_3:92870649-92870668 Msa0965480:CDS 40.0%
GTTGCAAATGTTGAAGAAGG+AGG - chr6_3:92870677-92870696 Msa0965480:CDS 40.0%
! CTTGATGATTTCTTCAGCAC+CGG + chr6_3:92870717-92870736 None:intergenic 40.0%
AATGTCGCGTACTTTAGACA+AGG - chr6_3:92870809-92870828 Msa0965480:five_prime_UTR 40.0%
!! GTGATTGGTGAGAGTGTTGT+TGG + chr6_3:92868826-92868845 None:intergenic 45.0%
AAGGTGACTGTTAGGGTGAT+TGG + chr6_3:92868841-92868860 None:intergenic 45.0%
ACCCTAACAGTCACCTTCAA+TGG - chr6_3:92868844-92868863 Msa0965480:three_prime_UTR 45.0%
GCCATTGAAGGTGACTGTTA+GGG + chr6_3:92868848-92868867 None:intergenic 45.0%
AGCCATTGAAGGTGACTGTT+AGG + chr6_3:92868849-92868868 None:intergenic 45.0%
!! GTCATCATCTTTGCTGTTCC+TGG - chr6_3:92868974-92868993 Msa0965480:CDS 45.0%
AACAGCTTGAAGCATGTTCC+TGG - chr6_3:92869777-92869796 Msa0965480:intron 45.0%
TCTGATCTCTCAATGAAGCC+AGG + chr6_3:92869798-92869817 None:intergenic 45.0%
GAGAGATCAGAAGAGCTGAA+AGG - chr6_3:92869807-92869826 Msa0965480:CDS 45.0%
GATACCTGTTGGATCGAGAT+TGG - chr6_3:92870243-92870262 Msa0965480:intron 45.0%
GCCCATGAATTCAGCACAAA+TGG + chr6_3:92870503-92870522 None:intergenic 45.0%
! CTTGTTCTCTGGGAATGTCT+TGG + chr6_3:92870525-92870544 None:intergenic 45.0%
CATGTCAAGTTCCTTGCTGA+TGG - chr6_3:92870545-92870564 Msa0965480:intron 45.0%
GCCAAATACACACATGCTCT+TGG - chr6_3:92870572-92870591 Msa0965480:intron 45.0%
! CCCAAGAGCATGTGTGTATT+TGG + chr6_3:92870576-92870595 None:intergenic 45.0%
ATACACACATGCTCTTGGGT+TGG - chr6_3:92870577-92870596 Msa0965480:intron 45.0%
TGTTGAAGACCTGAAGGTGA+AGG - chr6_3:92870655-92870674 Msa0965480:CDS 45.0%
CATTTGCAACCTTCACCTTC+AGG + chr6_3:92870667-92870686 None:intergenic 45.0%
! CGGTGCTGAAGAAATCATCA+AGG - chr6_3:92870715-92870734 Msa0965480:CDS 45.0%
!! GTGATTGGTGAGAGTGTTGT+TGG + chr6_3:92868826-92868845 None:intergenic 45.0%
AAGGTGACTGTTAGGGTGAT+TGG + chr6_3:92868841-92868860 None:intergenic 45.0%
ACCCTAACAGTCACCTTCAA+TGG - chr6_3:92868844-92868863 Msa0965480:three_prime_UTR 45.0%
GCCATTGAAGGTGACTGTTA+GGG + chr6_3:92868848-92868867 None:intergenic 45.0%
AGCCATTGAAGGTGACTGTT+AGG + chr6_3:92868849-92868868 None:intergenic 45.0%
!! GTCATCATCTTTGCTGTTCC+TGG - chr6_3:92868974-92868993 Msa0965480:CDS 45.0%
AACAGCTTGAAGCATGTTCC+TGG - chr6_3:92869777-92869796 Msa0965480:intron 45.0%
TCTGATCTCTCAATGAAGCC+AGG + chr6_3:92869798-92869817 None:intergenic 45.0%
GAGAGATCAGAAGAGCTGAA+AGG - chr6_3:92869807-92869826 Msa0965480:CDS 45.0%
GATACCTGTTGGATCGAGAT+TGG - chr6_3:92870243-92870262 Msa0965480:intron 45.0%
GCCCATGAATTCAGCACAAA+TGG + chr6_3:92870503-92870522 None:intergenic 45.0%
! CTTGTTCTCTGGGAATGTCT+TGG + chr6_3:92870525-92870544 None:intergenic 45.0%
CATGTCAAGTTCCTTGCTGA+TGG - chr6_3:92870545-92870564 Msa0965480:intron 45.0%
GCCAAATACACACATGCTCT+TGG - chr6_3:92870572-92870591 Msa0965480:intron 45.0%
! CCCAAGAGCATGTGTGTATT+TGG + chr6_3:92870576-92870595 None:intergenic 45.0%
ATACACACATGCTCTTGGGT+TGG - chr6_3:92870577-92870596 Msa0965480:intron 45.0%
TGTTGAAGACCTGAAGGTGA+AGG - chr6_3:92870655-92870674 Msa0965480:CDS 45.0%
CATTTGCAACCTTCACCTTC+AGG + chr6_3:92870667-92870686 None:intergenic 45.0%
! CGGTGCTGAAGAAATCATCA+AGG - chr6_3:92870715-92870734 Msa0965480:CDS 45.0%
!!! AATATTTTATTTCATTTTTA+GGG + chr6_3:92869058-92869077 None:intergenic 5.0%
!!! AATATTTTATTTCATTTTTA+GGG + chr6_3:92869058-92869077 None:intergenic 5.0%
GTAGGAGATGTGATCCCAGA+TGG - chr6_3:92868878-92868897 Msa0965480:three_prime_UTR 50.0%
ACCATTCATTCCCTCTCTGC+CGG - chr6_3:92868944-92868963 Msa0965480:CDS 50.0%
!!! GACTTTTTTGCCGGCAGAGA+GGG + chr6_3:92868957-92868976 None:intergenic 50.0%
!!! TGACTTTTTTGCCGGCAGAG+AGG + chr6_3:92868958-92868977 None:intergenic 50.0%
TAACCTGCATGTGGGTGTGA+AGG + chr6_3:92869002-92869021 None:intergenic 50.0%
CAGTCCAATCTCGATCCAAC+AGG + chr6_3:92870250-92870269 None:intergenic 50.0%
ATTTGGCAGAGCCATCAGCA+AGG + chr6_3:92870559-92870578 None:intergenic 50.0%
GGAGGAGAATACACCATCTC+CGG - chr6_3:92870695-92870714 Msa0965480:CDS 50.0%
! GATTTCTTCAGCACCGGAGA+TGG + chr6_3:92870711-92870730 None:intergenic 50.0%
GTAGGAGATGTGATCCCAGA+TGG - chr6_3:92868878-92868897 Msa0965480:three_prime_UTR 50.0%
ACCATTCATTCCCTCTCTGC+CGG - chr6_3:92868944-92868963 Msa0965480:CDS 50.0%
!!! GACTTTTTTGCCGGCAGAGA+GGG + chr6_3:92868957-92868976 None:intergenic 50.0%
!!! TGACTTTTTTGCCGGCAGAG+AGG + chr6_3:92868958-92868977 None:intergenic 50.0%
TAACCTGCATGTGGGTGTGA+AGG + chr6_3:92869002-92869021 None:intergenic 50.0%
CAGTCCAATCTCGATCCAAC+AGG + chr6_3:92870250-92870269 None:intergenic 50.0%
ATTTGGCAGAGCCATCAGCA+AGG + chr6_3:92870559-92870578 None:intergenic 50.0%
GGAGGAGAATACACCATCTC+CGG - chr6_3:92870695-92870714 Msa0965480:CDS 50.0%
! GATTTCTTCAGCACCGGAGA+TGG + chr6_3:92870711-92870730 None:intergenic 50.0%
! GGAAGGCTAAGGTGCCATCT+GGG + chr6_3:92868895-92868914 None:intergenic 55.0%
! AGGAAGGCTAAGGTGCCATC+TGG + chr6_3:92868896-92868915 None:intergenic 55.0%
GAGCTTGACCTCAGCGACAA+AGG - chr6_3:92870599-92870618 Msa0965480:intron 55.0%
GACCTCAGCGACAAAGGTCT+CGG - chr6_3:92870605-92870624 Msa0965480:intron 55.0%
! GGAAGGCTAAGGTGCCATCT+GGG + chr6_3:92868895-92868914 None:intergenic 55.0%
! AGGAAGGCTAAGGTGCCATC+TGG + chr6_3:92868896-92868915 None:intergenic 55.0%
GAGCTTGACCTCAGCGACAA+AGG - chr6_3:92870599-92870618 Msa0965480:intron 55.0%
GACCTCAGCGACAAAGGTCT+CGG - chr6_3:92870605-92870624 Msa0965480:intron 55.0%
GCCGGCAGAGAGGGAATGAA+TGG + chr6_3:92868948-92868967 None:intergenic 60.0%
!! CATGTGGGTGTGAAGGCACC+AGG + chr6_3:92868995-92869014 None:intergenic 60.0%
GTGCCTTCACACCCACATGC+AGG - chr6_3:92868996-92869015 Msa0965480:CDS 60.0%
TGCCGAGACCTTTGTCGCTG+AGG + chr6_3:92870610-92870629 None:intergenic 60.0%
GTCTCGGCATTCGCTCCAAG+AGG - chr6_3:92870621-92870640 Msa0965480:intron 60.0%
GCCGGCAGAGAGGGAATGAA+TGG + chr6_3:92868948-92868967 None:intergenic 60.0%
!! CATGTGGGTGTGAAGGCACC+AGG + chr6_3:92868995-92869014 None:intergenic 60.0%
GTGCCTTCACACCCACATGC+AGG - chr6_3:92868996-92869015 Msa0965480:CDS 60.0%
TGCCGAGACCTTTGTCGCTG+AGG + chr6_3:92870610-92870629 None:intergenic 60.0%
GTCTCGGCATTCGCTCCAAG+AGG - chr6_3:92870621-92870640 Msa0965480:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 92868716 92870943 92868716 ID=Msa0965480;Name=Msa0965480
chr6_3 mRNA 92868716 92870943 92868716 ID=Msa0965480-mRNA-1;Parent=Msa0965480;Name=Msa0965480-mRNA-1;_AED=0.03;_eAED=0.03;_QI=147|1|1|1|1|1|3|198|162
chr6_3 exon 92868716 92869167 92868716 ID=Msa0965480-mRNA-1:exon:16954;Parent=Msa0965480-mRNA-1
chr6_3 exon 92869798 92869877 92869798 ID=Msa0965480-mRNA-1:exon:16953;Parent=Msa0965480-mRNA-1
chr6_3 exon 92870642 92870943 92870642 ID=Msa0965480-mRNA-1:exon:16952;Parent=Msa0965480-mRNA-1
chr6_3 five_prime_UTR 92870797 92870943 92870797 ID=Msa0965480-mRNA-1:five_prime_utr;Parent=Msa0965480-mRNA-1
chr6_3 CDS 92870642 92870796 92870642 ID=Msa0965480-mRNA-1:cds;Parent=Msa0965480-mRNA-1
chr6_3 CDS 92869798 92869877 92869798 ID=Msa0965480-mRNA-1:cds;Parent=Msa0965480-mRNA-1
chr6_3 CDS 92868914 92869167 92868914 ID=Msa0965480-mRNA-1:cds;Parent=Msa0965480-mRNA-1
chr6_3 three_prime_UTR 92868716 92868913 92868716 ID=Msa0965480-mRNA-1:three_prime_utr;Parent=Msa0965480-mRNA-1
Gene Sequence

>Msa0965480

ATGGCTCCAATCAAAGTAGGAGATGTGATCCCAGATGGCACCTTAGCCTTCCTTGATGAAGAAAACAAACCTCAATCTGTCACCATTCATTCCCTCTCTGCCGGCAAAAAAGTCATCATCTTTGCTGTTCCTGGTGCCTTCACACCCACATGCAGCTTGAAGCATGTTCCTGGCTTCATTGAGAGATCAGAAGAGCTGAAAGGAAAGGGTGTTGATGAAATCATCTGCATCAGTGTCAATGATCCATTTGTGCTGAATTCATGGGCCAAGACATTCCCAGAGAACAAGCATGTCAAGTTCCTTGCTGATGGCTCTGCCAAATACACACATGCTCTTGGGTTGGAGCTTGACCTCAGCGACAAAGGTCTCGGCATTCGCTCCAAGAGGTTTGCTCTTCTTGTTGAAGACCTGAAGGTGAAGGTTGCAAATGTTGAAGAAGGAGGAGAATACACCATCTCCGGTGCTGAAGAAATCATCAAGGCTCTTTGA
Protein sequence

>Msa0965480

MAPIKVGDVIPDGTLAFLDEENKPQSVTIHSLSAGKKVIIFAVPGAFTPTCSLKHVPGFIERSEELKGKGVDEIICISVNDPFVLNSWAKTFPENKHVKFLADGSAKYTHALGLELDLSDKGLGIRSKRFALLVEDLKVKVANVEEGGEYTISGAEEIIKAL*