AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0330440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0330440 MtrunA17_Chr2g0312771 90.187 214 21 0 1 214 1 214 4.41e-147 408
Msa0330440 MtrunA17_Chr2g0312781 89.401 217 23 0 1 217 18 234 4.27e-146 406
Msa0330440 MtrunA17_Chr2g0312751 88.785 214 24 0 1 214 1 214 2.41e-145 404
Msa0330440 MtrunA17_Chr2g0312731 79.167 216 45 0 1 216 1 216 1.11e-132 372
Msa0330440 MtrunA17_Chr2g0312811 77.315 216 47 1 1 214 1 216 1.01e-126 357
Msa0330440 MtrunA17_Chr2g0312791 73.516 219 55 3 1 216 1 219 2.84e-118 335
Msa0330440 MtrunA17_Chr2g0312681 65.116 215 75 0 1 215 1 215 4.73e-111 317
Msa0330440 MtrunA17_Chr2g0312691 64.651 215 76 0 1 215 1 215 3.80e-107 307
Msa0330440 MtrunA17_Chr2g0312711 63.256 215 78 1 1 215 1 214 2.49e-101 292
Msa0330440 MtrunA17_Chr8g0357241 64.486 214 76 0 1 214 1 214 3.98e-101 291
Msa0330440 MtrunA17_Chr8g0357211 63.850 213 77 0 1 213 1 213 4.10e-100 289
Msa0330440 MtrunA17_Chr3g0107731 61.905 210 80 0 5 214 10 219 7.14e-100 289
Msa0330440 MtrunA17_Chr3g0107741 59.906 212 85 0 3 214 8 219 3.19e-96 280
Msa0330440 MtrunA17_Chr2g0312721 60.094 213 85 0 1 213 1 213 9.85e-93 270
Msa0330440 MtrunA17_Chr5g0433801 52.336 214 100 2 2 214 4 216 3.85e-80 239
Msa0330440 MtrunA17_Chr4g0069471 61.749 183 67 2 1 182 22 202 6.13e-80 238
Msa0330440 MtrunA17_Chr1g0196661 41.905 210 117 3 4 209 3 211 3.00e-51 165
Msa0330440 MtrunA17_Chr8g0379581 40.741 216 125 2 2 215 3 217 3.43e-51 165
Msa0330440 MtrunA17_Chr7g0240681 41.429 210 117 4 3 208 6 213 6.76e-51 164
Msa0330440 MtrunA17_Chr5g0415411 41.818 220 119 5 2 212 3 222 9.37e-51 164
Msa0330440 MtrunA17_Chr7g0240691 42.857 210 114 4 3 208 36 243 6.72e-50 163
Msa0330440 MtrunA17_Chr4g0008781 40.670 209 121 2 3 209 7 214 1.43e-49 160
Msa0330440 MtrunA17_Chr3g0132581 41.827 208 118 2 3 208 4 210 1.88e-49 160
Msa0330440 MtrunA17_Chr8g0379561 42.029 207 117 2 4 208 6 211 3.10e-49 160
Msa0330440 MtrunA17_Chr7g0240641 40.952 210 119 4 3 209 6 213 3.85e-49 160
Msa0330440 MtrunA17_Chr5g0417011 36.493 211 127 2 2 205 5 215 4.68e-49 159
Msa0330440 MtrunA17_Chr7g0240611 41.429 210 114 5 3 209 6 209 6.93e-49 159
Msa0330440 MtrunA17_Chr7g0240631 39.524 210 122 4 3 209 6 213 3.47e-48 157
Msa0330440 MtrunA17_Chr4g0029341 42.381 210 114 4 1 205 1 208 6.11e-48 156
Msa0330440 MtrunA17_Chr4g0008791 41.379 203 114 4 4 203 7 207 2.72e-46 152
Msa0330440 MtrunA17_Chr6g0483141 41.753 194 108 3 18 208 17 208 3.88e-46 152
Msa0330440 MtrunA17_Chr1g0196691 38.605 215 124 5 4 212 28 240 6.22e-46 152
Msa0330440 MtrunA17_Chr7g0240671 40.000 210 121 5 1 209 1 206 8.91e-46 151
Msa0330440 MtrunA17_Chr1g0196771 37.788 217 129 4 3 215 2 216 1.24e-45 151
Msa0330440 MtrunA17_Chr1g0196721 42.211 199 110 3 11 205 9 206 7.90e-45 149
Msa0330440 MtrunA17_Chr7g0240531 41.262 206 116 5 3 205 6 209 1.08e-44 148
Msa0330440 MtrunA17_Chr7g0240561 38.725 204 120 4 3 203 6 207 1.20e-44 148
Msa0330440 MtrunA17_Chr7g0240581 38.571 210 124 4 3 209 6 213 4.25e-44 147
Msa0330440 MtrunA17_Chr7g0240541 40.196 204 117 5 3 203 6 207 1.47e-43 145
Msa0330440 MtrunA17_Chr7g0240551 40.777 206 117 5 3 205 6 209 1.61e-43 145
Msa0330440 MtrunA17_Chr7g0240661 40.952 210 119 5 1 209 4 209 2.59e-43 145
Msa0330440 MtrunA17_Chr7g0240651 40.476 210 116 5 3 209 6 209 6.08e-43 144
Msa0330440 MtrunA17_Chr8g0363751 35.047 214 124 4 4 205 5 215 9.27e-42 141
Msa0330440 MtrunA17_Chr7g0240511 40.394 203 113 5 3 203 6 202 4.45e-41 139
Msa0330440 MtrunA17_Chr1g0196711 41.262 206 116 4 4 205 3 207 5.72e-41 139
Msa0330440 MtrunA17_Chr1g0212301 39.336 211 119 6 1 205 1 208 5.33e-39 134
Msa0330440 MtrunA17_Chr7g0240521 38.806 201 118 4 3 200 13 211 5.61e-37 129
Msa0330440 MtrunA17_Chr8g0391631 39.873 158 82 3 4 151 21 175 5.88e-36 124
Msa0330440 MtrunA17_Chr2g0313561 60.811 74 28 1 47 119 1 74 8.37e-30 105
Msa0330440 MtrunA17_Chr4g0076691 30.994 171 99 5 4 159 15 181 1.22e-13 67.4
Msa0330440 MtrunA17_Chr1g0181861 28.431 204 131 7 10 207 33 227 7.64e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0330440 AT1G78380.1 68.692 214 67 0 1 214 1 214 2.29e-112 320
Msa0330440 AT1G17180.1 66.822 214 71 0 1 214 1 214 2.01e-111 318
Msa0330440 AT1G78370.1 65.278 216 75 0 1 216 1 216 2.71e-107 308
Msa0330440 AT1G78360.1 65.116 215 74 1 1 214 1 215 9.87e-105 301
Msa0330440 AT1G17170.1 64.486 214 75 1 1 214 1 213 1.56e-104 301
Msa0330440 AT1G78340.1 63.551 214 78 0 1 214 1 214 2.16e-104 300
Msa0330440 AT1G78320.1 64.019 214 77 0 1 214 1 214 4.47e-104 300
Msa0330440 AT1G17190.1 58.019 212 89 0 3 214 4 215 1.55e-94 275
Msa0330440 AT1G53680.1 58.019 212 87 2 5 214 8 219 4.38e-93 272
Msa0330440 AT3G43800.1 54.839 217 94 3 3 215 4 220 1.48e-83 248
Msa0330440 AT1G78320.2 55.607 214 68 1 1 214 1 187 2.37e-82 243
Msa0330440 AT2G29420.1 43.810 210 115 2 2 209 7 215 2.86e-59 186
Msa0330440 AT3G09270.1 42.718 206 115 2 3 205 5 210 9.63e-53 169
Msa0330440 AT2G29440.1 44.660 206 108 4 3 204 5 208 2.17e-51 166
Msa0330440 AT2G29450.1 44.444 207 108 5 3 204 5 209 1.21e-50 164
Msa0330440 AT1G10360.1 40.639 219 122 4 2 212 3 221 3.60e-50 163
Msa0330440 AT2G29490.1 39.171 217 126 4 3 215 6 220 9.42e-50 162
Msa0330440 AT1G59700.1 41.096 219 120 5 3 212 5 223 3.14e-48 158
Msa0330440 AT2G29480.1 40.777 206 116 4 3 204 6 209 5.70e-48 157
Msa0330440 AT2G29470.1 38.967 213 123 5 5 212 8 218 4.13e-47 155
Msa0330440 AT1G10370.1 37.838 222 116 4 2 208 3 217 6.46e-46 152
Msa0330440 AT2G29460.1 39.806 206 118 4 3 204 6 209 7.61e-46 152
Msa0330440 AT1G59670.1 40.909 220 119 5 3 212 5 223 6.65e-45 149
Msa0330440 AT1G74590.1 35.000 220 129 3 5 214 8 223 4.61e-44 147
Msa0330440 AT1G27140.1 37.778 225 130 4 3 217 5 229 1.46e-43 146
Msa0330440 AT1G27130.1 37.264 212 124 4 3 205 5 216 8.52e-43 144
Msa0330440 AT1G69930.1 36.967 211 126 3 5 208 13 223 1.47e-42 144
Msa0330440 AT5G62480.1 41.243 177 94 2 1 168 5 180 6.89e-42 142
Msa0330440 AT1G69920.1 37.019 208 116 5 5 200 35 239 1.06e-39 137
Msa0330440 AT5G62480.2 33.714 175 80 3 3 168 7 154 1.88e-25 99.0
Msa0330440 AT5G62480.3 33.714 175 79 3 3 168 7 153 1.95e-25 99.0

Find 83 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAAAGGGGTTTAGATTT+AGG 0.118584 2_4:+44829079 None:intergenic
TTGGGAGACAAGACTTATTT+TGG 0.161950 2_4:-44829652 Msa0330440:CDS
TGAAAAGGGGTTTAGATTTA+GGG 0.253900 2_4:+44829080 None:intergenic
GTGTGACATTTGTTAAAATT+AGG 0.258038 2_4:-44829342 Msa0330440:three_prime_UTR
GCAACTAAAGATTCACAAAT+AGG 0.275943 2_4:+44830116 None:intergenic
TATTATATTATGTAATAAAA+AGG 0.285708 2_4:-44829236 Msa0330440:three_prime_UTR
GATTAGATCAACGGTGAGTT+TGG 0.293087 2_4:-44829388 Msa0330440:three_prime_UTR
CAAATTGTTGGAGCAAGAAT+TGG 0.293479 2_4:-44829671 Msa0330440:CDS
GACATCTATGAATTCTTTCT+TGG 0.300931 2_4:+44829696 None:intergenic
TAGGATGGTTTATTGTGATT+TGG 0.304834 2_4:-44829323 Msa0330440:three_prime_UTR
AAGTGATTCTGCTTGATTCC+TGG 0.317076 2_4:-44830292 Msa0330440:CDS
TCAAATGAGTGGTTATATAT+AGG 0.323189 2_4:+44829115 None:intergenic
TTCAGTATATTGATGAGGTT+TGG 0.327749 2_4:-44830091 Msa0330440:CDS
TTTATTTATAGAGTGAGATT+TGG 0.329312 2_4:+44830419 None:intergenic
TAAATCATTTATGACGTGTT+TGG 0.334319 2_4:+44829286 None:intergenic
AAATCTCACTCTATAAATAA+AGG 0.355663 2_4:-44830417 Msa0330440:five_prime_UTR
CAAATGAGTGGTTATATATA+GGG 0.356990 2_4:+44829116 None:intergenic
TTTAAAAGCTATGAGACCTT+TGG 0.358405 2_4:-44829577 Msa0330440:CDS
AAGGAACCTTTGGGCCAAAA+AGG 0.360471 2_4:-44829737 Msa0330440:CDS
CTTACAAAATGCACAACGTT+TGG 0.364730 2_4:+44829201 None:intergenic
GATTCCTGGGCAAGTCCATT+TGG 0.366880 2_4:-44830278 Msa0330440:CDS
CATAGATGTCCTCAAATTGT+TGG 0.381980 2_4:-44829683 Msa0330440:CDS
CAGAATAGCCCTAGCTGAAA+AGG 0.384656 2_4:-44830246 Msa0330440:CDS
ATGAAGGTGGAAGGAACCTT+TGG 0.387307 2_4:-44829747 Msa0330440:CDS
CACTTATTCCATTCTACACT+TGG 0.395366 2_4:-44829600 Msa0330440:CDS
ATTCTTGCTCCAACAATTTG+AGG 0.395572 2_4:+44829674 None:intergenic
TCATGGACCTTATCCTGATC+AGG 0.413575 2_4:+44829469 None:intergenic
AGACGGGTTATTGAGTAGGT+TGG 0.414239 2_4:-44829426 Msa0330440:exon
TTACCGTTATGAATCAGAAC+AGG 0.415381 2_4:+44830140 None:intergenic
CTCAATCTGCATACATCTCT+TGG 0.415648 2_4:+44829510 None:intergenic
AGAGATCACAAGCTAGGTTC+TGG 0.428783 2_4:-44830031 Msa0330440:CDS
ATAGAATAGATAGATTGTTA+TGG 0.430247 2_4:+44830357 None:intergenic
TTGGACCAAGCAATGAACTT+AGG 0.437629 2_4:+44829529 None:intergenic
TATGTTGAATTGCACAATAT+AGG 0.449373 2_4:-44830530 None:intergenic
TTCAAAGTCACTCCCTGATC+AGG 0.452471 2_4:-44829482 Msa0330440:CDS
CTAGCTTGTGATCTCTGATA+AGG 0.467136 2_4:+44830038 None:intergenic
TGGTGTGTGGAATGTGGAAT+AGG 0.467678 2_4:+44830461 None:intergenic
GGTTCATTTGTAACAACAAA+GGG 0.474781 2_4:+44830180 None:intergenic
TGGACCAAGCAATGAACTTA+GGG 0.476815 2_4:+44829530 None:intergenic
AAATTGTTGGAGCAAGAATT+GGG 0.479522 2_4:-44829670 Msa0330440:CDS
AATGTGTGTGAGAGAGTACA+AGG 0.479771 2_4:+44830387 None:intergenic
AGTGATTCTGCTTGATTCCT+GGG 0.480041 2_4:-44830291 Msa0330440:CDS
CTCTAAATTGATGTTTCCAA+AGG 0.489801 2_4:+44829561 None:intergenic
GAGATCACAAGCTAGGTTCT+GGG 0.491244 2_4:-44830030 Msa0330440:CDS
AATGCCCTAAGTTCATTGCT+TGG 0.495645 2_4:-44829534 Msa0330440:CDS
GACATTTGTTAAAATTAGGA+TGG 0.497466 2_4:-44829338 Msa0330440:three_prime_UTR
TGAAGGTGGAAGGAACCTTT+GGG 0.498294 2_4:-44829746 Msa0330440:CDS
GTCACTCCCTGATCAGGATA+AGG 0.500395 2_4:-44829476 Msa0330440:CDS
GGCTGATTATGTTGATAAGA+AGG 0.507102 2_4:-44830009 Msa0330440:intron
ATTCTGAGTCTCATGCCAAA+TGG 0.515676 2_4:+44830263 None:intergenic
AATGTCAAATAGATCTATGA+AGG 0.517536 2_4:-44829763 Msa0330440:intron
TGGAAACATCAATTTAGAGA+AGG 0.525535 2_4:-44829557 Msa0330440:CDS
AGAATAGCCCTAGCTGAAAA+GGG 0.531819 2_4:-44830245 Msa0330440:CDS
ATTGTAGAGCTGAGGAAGAC+GGG 0.532079 2_4:-44829442 Msa0330440:CDS
CATGCCAAATGGACTTGCCC+AGG 0.533387 2_4:+44830274 None:intergenic
AGGAAGACGGGTTATTGAGT+AGG 0.546317 2_4:-44829430 Msa0330440:exon
CTTATCAGAGATCACAAGCT+AGG 0.551577 2_4:-44830037 Msa0330440:CDS
AATAGATAGATTGTTATGGA+AGG 0.553152 2_4:+44830361 None:intergenic
CATTGTAGAGCTGAGGAAGA+CGG 0.561187 2_4:-44829443 Msa0330440:CDS
ATCCCTGTTCTGATTCATAA+CGG 0.561977 2_4:-44830143 Msa0330440:CDS
TTATTCTCTCTGACTAGCCA+TGG 0.562818 2_4:-44830321 Msa0330440:exon
TGCTGTTCAGTATATTGATG+AGG 0.563629 2_4:-44830096 Msa0330440:CDS
TACACACTTGTGAATTGTGA+TGG 0.564133 2_4:-44829164 Msa0330440:three_prime_UTR
AGGAAGGAATAGTGACTGTG+AGG 0.567594 2_4:+44830481 None:intergenic
GTCAAATAGATCTATGAAGG+TGG 0.573200 2_4:-44829760 Msa0330440:intron
GTGGTTATATATAGGGACGG+AGG 0.582958 2_4:+44829123 None:intergenic
AATAGATCTATGAAGGTGGA+AGG 0.587856 2_4:-44829756 Msa0330440:intron
CAGAATCACTTCATCAGCCA+TGG 0.592382 2_4:+44830304 None:intergenic
GAAATGTGGTGTGTGGAATG+TGG 0.594195 2_4:+44830455 None:intergenic
GATCTCTGATAAGGATCAGA+AGG 0.596783 2_4:+44830047 None:intergenic
TGGTTATATATAGGGACGGA+GGG 0.597800 2_4:+44829124 None:intergenic
GGGTTCATTTGTAACAACAA+AGG 0.598423 2_4:+44830179 None:intergenic
TCAGCTCTACAATGTAGTCA+TGG 0.600557 2_4:+44829452 None:intergenic
CATGGACCTTATCCTGATCA+GGG 0.606504 2_4:+44829470 None:intergenic
TAAGGATCAGAAGGCAACAA+AGG 0.613337 2_4:+44830056 None:intergenic
TAAGTGCATGATTAGATCAA+CGG 0.615913 2_4:-44829397 Msa0330440:three_prime_UTR
TACCGTTATGAATCAGAACA+GGG 0.629965 2_4:+44830141 None:intergenic
GTGTGGAATGTGGAATAGGA+AGG 0.630377 2_4:+44830465 None:intergenic
ATGACTACATTGTAGAGCTG+AGG 0.637957 2_4:-44829450 Msa0330440:CDS
TAGTATAGAAATGTGGTGTG+TGG 0.643154 2_4:+44830448 None:intergenic
TGAGTGGTTATATATAGGGA+CGG 0.649729 2_4:+44829120 None:intergenic
ATGTGTGTGAGAGAGTACAA+GGG 0.660733 2_4:+44830388 None:intergenic
GAATGAATAGTATAGAAATG+TGG 0.685822 2_4:+44830441 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTATAGAGTGAGATT+TGG + chr2_4:44829194-44829213 None:intergenic 20.0%
!! AAATCTCACTCTATAAATAA+AGG - chr2_4:44829193-44829212 Msa0330440:three_prime_UTR 20.0%
!! ATAGAATAGATAGATTGTTA+TGG + chr2_4:44829256-44829275 None:intergenic 20.0%
!!! TGATAAGAAGGTATATAAAT+TGG - chr2_4:44829613-44829632 Msa0330440:CDS 20.0%
!!! AGTTTTATATGAAACTTGTA+TGG - chr2_4:44829764-44829783 Msa0330440:intron 20.0%
!! TTTATTTATAGAGTGAGATT+TGG + chr2_4:44829194-44829213 None:intergenic 20.0%
!! AAATCTCACTCTATAAATAA+AGG - chr2_4:44829193-44829212 Msa0330440:three_prime_UTR 20.0%
!! ATAGAATAGATAGATTGTTA+TGG + chr2_4:44829256-44829275 None:intergenic 20.0%
!!! TGATAAGAAGGTATATAAAT+TGG - chr2_4:44829613-44829632 Msa0330440:CDS 20.0%
!!! AGTTTTATATGAAACTTGTA+TGG - chr2_4:44829764-44829783 Msa0330440:intron 20.0%
! GAATGAATAGTATAGAAATG+TGG + chr2_4:44829172-44829191 None:intergenic 25.0%
! AATAGATAGATTGTTATGGA+AGG + chr2_4:44829252-44829271 None:intergenic 25.0%
! AATGTCAAATAGATCTATGA+AGG - chr2_4:44829847-44829866 Msa0330440:intron 25.0%
!! GTGTGACATTTGTTAAAATT+AGG - chr2_4:44830268-44830287 Msa0330440:CDS 25.0%
!! GACATTTGTTAAAATTAGGA+TGG - chr2_4:44830272-44830291 Msa0330440:CDS 25.0%
! TAAATCATTTATGACGTGTT+TGG + chr2_4:44830327-44830346 None:intergenic 25.0%
! CAAATGAGTGGTTATATATA+GGG + chr2_4:44830497-44830516 None:intergenic 25.0%
! TCAAATGAGTGGTTATATAT+AGG + chr2_4:44830498-44830517 None:intergenic 25.0%
!!! CAATTTTTTAGTCAAATGAG+TGG + chr2_4:44830509-44830528 None:intergenic 25.0%
! GAATGAATAGTATAGAAATG+TGG + chr2_4:44829172-44829191 None:intergenic 25.0%
! AATAGATAGATTGTTATGGA+AGG + chr2_4:44829252-44829271 None:intergenic 25.0%
! AATGTCAAATAGATCTATGA+AGG - chr2_4:44829847-44829866 Msa0330440:intron 25.0%
!! GTGTGACATTTGTTAAAATT+AGG - chr2_4:44830268-44830287 Msa0330440:CDS 25.0%
!! GACATTTGTTAAAATTAGGA+TGG - chr2_4:44830272-44830291 Msa0330440:CDS 25.0%
! TAAATCATTTATGACGTGTT+TGG + chr2_4:44830327-44830346 None:intergenic 25.0%
! CAAATGAGTGGTTATATATA+GGG + chr2_4:44830497-44830516 None:intergenic 25.0%
! TCAAATGAGTGGTTATATAT+AGG + chr2_4:44830498-44830517 None:intergenic 25.0%
!!! CAATTTTTTAGTCAAATGAG+TGG + chr2_4:44830509-44830528 None:intergenic 25.0%
GGTTCATTTGTAACAACAAA+GGG + chr2_4:44829433-44829452 None:intergenic 30.0%
GCAACTAAAGATTCACAAAT+AGG + chr2_4:44829497-44829516 None:intergenic 30.0%
TTCAGTATATTGATGAGGTT+TGG - chr2_4:44829519-44829538 Msa0330440:CDS 30.0%
GACATCTATGAATTCTTTCT+TGG + chr2_4:44829917-44829936 None:intergenic 30.0%
AAATTGTTGGAGCAAGAATT+GGG - chr2_4:44829940-44829959 Msa0330440:intron 30.0%
! CTTTTAAACCAAGTGTAGAA+TGG + chr2_4:44830021-44830040 None:intergenic 30.0%
TTTAAAAGCTATGAGACCTT+TGG - chr2_4:44830033-44830052 Msa0330440:CDS 30.0%
CTCTAAATTGATGTTTCCAA+AGG + chr2_4:44830052-44830071 None:intergenic 30.0%
TGGAAACATCAATTTAGAGA+AGG - chr2_4:44830053-44830072 Msa0330440:CDS 30.0%
TAAGTGCATGATTAGATCAA+CGG - chr2_4:44830213-44830232 Msa0330440:CDS 30.0%
TAGGATGGTTTATTGTGATT+TGG - chr2_4:44830287-44830306 Msa0330440:CDS 30.0%
GGTTCATTTGTAACAACAAA+GGG + chr2_4:44829433-44829452 None:intergenic 30.0%
GCAACTAAAGATTCACAAAT+AGG + chr2_4:44829497-44829516 None:intergenic 30.0%
TTCAGTATATTGATGAGGTT+TGG - chr2_4:44829519-44829538 Msa0330440:CDS 30.0%
GACATCTATGAATTCTTTCT+TGG + chr2_4:44829917-44829936 None:intergenic 30.0%
AAATTGTTGGAGCAAGAATT+GGG - chr2_4:44829940-44829959 Msa0330440:intron 30.0%
! CTTTTAAACCAAGTGTAGAA+TGG + chr2_4:44830021-44830040 None:intergenic 30.0%
TTTAAAAGCTATGAGACCTT+TGG - chr2_4:44830033-44830052 Msa0330440:CDS 30.0%
CTCTAAATTGATGTTTCCAA+AGG + chr2_4:44830052-44830071 None:intergenic 30.0%
TGGAAACATCAATTTAGAGA+AGG - chr2_4:44830053-44830072 Msa0330440:CDS 30.0%
TAAGTGCATGATTAGATCAA+CGG - chr2_4:44830213-44830232 Msa0330440:CDS 30.0%
TAGGATGGTTTATTGTGATT+TGG - chr2_4:44830287-44830306 Msa0330440:CDS 30.0%
TAGTATAGAAATGTGGTGTG+TGG + chr2_4:44829165-44829184 None:intergenic 35.0%
GGGTTCATTTGTAACAACAA+AGG + chr2_4:44829434-44829453 None:intergenic 35.0%
ATCCCTGTTCTGATTCATAA+CGG - chr2_4:44829467-44829486 Msa0330440:CDS 35.0%
TACCGTTATGAATCAGAACA+GGG + chr2_4:44829472-44829491 None:intergenic 35.0%
TTACCGTTATGAATCAGAAC+AGG + chr2_4:44829473-44829492 None:intergenic 35.0%
TGCTGTTCAGTATATTGATG+AGG - chr2_4:44829514-44829533 Msa0330440:CDS 35.0%
! GGCTGATTATGTTGATAAGA+AGG - chr2_4:44829601-44829620 Msa0330440:CDS 35.0%
GTCAAATAGATCTATGAAGG+TGG - chr2_4:44829850-44829869 Msa0330440:intron 35.0%
AATAGATCTATGAAGGTGGA+AGG - chr2_4:44829854-44829873 Msa0330440:intron 35.0%
CATAGATGTCCTCAAATTGT+TGG - chr2_4:44829927-44829946 Msa0330440:intron 35.0%
ATTCTTGCTCCAACAATTTG+AGG + chr2_4:44829939-44829958 None:intergenic 35.0%
CAAATTGTTGGAGCAAGAAT+TGG - chr2_4:44829939-44829958 Msa0330440:intron 35.0%
! TTGGGAGACAAGACTTATTT+TGG - chr2_4:44829958-44829977 Msa0330440:intron 35.0%
!! TATTTTGGAGGAGATAAGCT+TGG - chr2_4:44829973-44829992 Msa0330440:intron 35.0%
CACTTATTCCATTCTACACT+TGG - chr2_4:44830010-44830029 Msa0330440:CDS 35.0%
CTTACAAAATGCACAACGTT+TGG + chr2_4:44830412-44830431 None:intergenic 35.0%
TACACACTTGTGAATTGTGA+TGG - chr2_4:44830446-44830465 Msa0330440:five_prime_UTR 35.0%
TGAGTGGTTATATATAGGGA+CGG + chr2_4:44830493-44830512 None:intergenic 35.0%
TAGTATAGAAATGTGGTGTG+TGG + chr2_4:44829165-44829184 None:intergenic 35.0%
GGGTTCATTTGTAACAACAA+AGG + chr2_4:44829434-44829453 None:intergenic 35.0%
ATCCCTGTTCTGATTCATAA+CGG - chr2_4:44829467-44829486 Msa0330440:CDS 35.0%
TACCGTTATGAATCAGAACA+GGG + chr2_4:44829472-44829491 None:intergenic 35.0%
TTACCGTTATGAATCAGAAC+AGG + chr2_4:44829473-44829492 None:intergenic 35.0%
TGCTGTTCAGTATATTGATG+AGG - chr2_4:44829514-44829533 Msa0330440:CDS 35.0%
! GGCTGATTATGTTGATAAGA+AGG - chr2_4:44829601-44829620 Msa0330440:CDS 35.0%
GTCAAATAGATCTATGAAGG+TGG - chr2_4:44829850-44829869 Msa0330440:intron 35.0%
AATAGATCTATGAAGGTGGA+AGG - chr2_4:44829854-44829873 Msa0330440:intron 35.0%
CATAGATGTCCTCAAATTGT+TGG - chr2_4:44829927-44829946 Msa0330440:intron 35.0%
ATTCTTGCTCCAACAATTTG+AGG + chr2_4:44829939-44829958 None:intergenic 35.0%
CAAATTGTTGGAGCAAGAAT+TGG - chr2_4:44829939-44829958 Msa0330440:intron 35.0%
! TTGGGAGACAAGACTTATTT+TGG - chr2_4:44829958-44829977 Msa0330440:intron 35.0%
!! TATTTTGGAGGAGATAAGCT+TGG - chr2_4:44829973-44829992 Msa0330440:intron 35.0%
CACTTATTCCATTCTACACT+TGG - chr2_4:44830010-44830029 Msa0330440:CDS 35.0%
CTTACAAAATGCACAACGTT+TGG + chr2_4:44830412-44830431 None:intergenic 35.0%
TACACACTTGTGAATTGTGA+TGG - chr2_4:44830446-44830465 Msa0330440:five_prime_UTR 35.0%
TGAGTGGTTATATATAGGGA+CGG + chr2_4:44830493-44830512 None:intergenic 35.0%
! ATGTGTGTGAGAGAGTACAA+GGG + chr2_4:44829225-44829244 None:intergenic 40.0%
! AATGTGTGTGAGAGAGTACA+AGG + chr2_4:44829226-44829245 None:intergenic 40.0%
! TTATTCTCTCTGACTAGCCA+TGG - chr2_4:44829289-44829308 Msa0330440:three_prime_UTR 40.0%
AAGTGATTCTGCTTGATTCC+TGG - chr2_4:44829318-44829337 Msa0330440:three_prime_UTR 40.0%
AGTGATTCTGCTTGATTCCT+GGG - chr2_4:44829319-44829338 Msa0330440:three_prime_UTR 40.0%
ATTCTGAGTCTCATGCCAAA+TGG + chr2_4:44829350-44829369 None:intergenic 40.0%
AGAATAGCCCTAGCTGAAAA+GGG - chr2_4:44829365-44829384 Msa0330440:three_prime_UTR 40.0%
! CTTGATACCCTTTTCAGCTA+GGG + chr2_4:44829375-44829394 None:intergenic 40.0%
! CAGGGATTTTCTTGTGAACA+GGG + chr2_4:44829454-44829473 None:intergenic 40.0%
! ACAGGGATTTTCTTGTGAAC+AGG + chr2_4:44829455-44829474 None:intergenic 40.0%
TAAGGATCAGAAGGCAACAA+AGG + chr2_4:44829557-44829576 None:intergenic 40.0%
GATCTCTGATAAGGATCAGA+AGG + chr2_4:44829566-44829585 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG + chr2_4:44829575-44829594 None:intergenic 40.0%
CTTATCAGAGATCACAAGCT+AGG - chr2_4:44829573-44829592 Msa0330440:CDS 40.0%
! TTCTTGCTCTTCTCCCTTTT+TGG + chr2_4:44829890-44829909 None:intergenic 40.0%
!!! GGAGACAAGACTTATTTTGG+AGG - chr2_4:44829961-44829980 Msa0330440:intron 40.0%
AATGCCCTAAGTTCATTGCT+TGG - chr2_4:44830076-44830095 Msa0330440:CDS 40.0%
TGGACCAAGCAATGAACTTA+GGG + chr2_4:44830083-44830102 None:intergenic 40.0%
TTGGACCAAGCAATGAACTT+AGG + chr2_4:44830084-44830103 None:intergenic 40.0%
CTCAATCTGCATACATCTCT+TGG + chr2_4:44830103-44830122 None:intergenic 40.0%
TCAGCTCTACAATGTAGTCA+TGG + chr2_4:44830161-44830180 None:intergenic 40.0%
ATGACTACATTGTAGAGCTG+AGG - chr2_4:44830160-44830179 Msa0330440:CDS 40.0%
GATTAGATCAACGGTGAGTT+TGG - chr2_4:44830222-44830241 Msa0330440:CDS 40.0%
TGGTTATATATAGGGACGGA+GGG + chr2_4:44830489-44830508 None:intergenic 40.0%
! ATGTGTGTGAGAGAGTACAA+GGG + chr2_4:44829225-44829244 None:intergenic 40.0%
! AATGTGTGTGAGAGAGTACA+AGG + chr2_4:44829226-44829245 None:intergenic 40.0%
! TTATTCTCTCTGACTAGCCA+TGG - chr2_4:44829289-44829308 Msa0330440:three_prime_UTR 40.0%
AAGTGATTCTGCTTGATTCC+TGG - chr2_4:44829318-44829337 Msa0330440:three_prime_UTR 40.0%
AGTGATTCTGCTTGATTCCT+GGG - chr2_4:44829319-44829338 Msa0330440:three_prime_UTR 40.0%
ATTCTGAGTCTCATGCCAAA+TGG + chr2_4:44829350-44829369 None:intergenic 40.0%
AGAATAGCCCTAGCTGAAAA+GGG - chr2_4:44829365-44829384 Msa0330440:three_prime_UTR 40.0%
! CTTGATACCCTTTTCAGCTA+GGG + chr2_4:44829375-44829394 None:intergenic 40.0%
! CAGGGATTTTCTTGTGAACA+GGG + chr2_4:44829454-44829473 None:intergenic 40.0%
! ACAGGGATTTTCTTGTGAAC+AGG + chr2_4:44829455-44829474 None:intergenic 40.0%
TAAGGATCAGAAGGCAACAA+AGG + chr2_4:44829557-44829576 None:intergenic 40.0%
GATCTCTGATAAGGATCAGA+AGG + chr2_4:44829566-44829585 None:intergenic 40.0%
! CTAGCTTGTGATCTCTGATA+AGG + chr2_4:44829575-44829594 None:intergenic 40.0%
CTTATCAGAGATCACAAGCT+AGG - chr2_4:44829573-44829592 Msa0330440:CDS 40.0%
! TTCTTGCTCTTCTCCCTTTT+TGG + chr2_4:44829890-44829909 None:intergenic 40.0%
!!! GGAGACAAGACTTATTTTGG+AGG - chr2_4:44829961-44829980 Msa0330440:intron 40.0%
AATGCCCTAAGTTCATTGCT+TGG - chr2_4:44830076-44830095 Msa0330440:CDS 40.0%
TGGACCAAGCAATGAACTTA+GGG + chr2_4:44830083-44830102 None:intergenic 40.0%
TTGGACCAAGCAATGAACTT+AGG + chr2_4:44830084-44830103 None:intergenic 40.0%
CTCAATCTGCATACATCTCT+TGG + chr2_4:44830103-44830122 None:intergenic 40.0%
TCAGCTCTACAATGTAGTCA+TGG + chr2_4:44830161-44830180 None:intergenic 40.0%
ATGACTACATTGTAGAGCTG+AGG - chr2_4:44830160-44830179 Msa0330440:CDS 40.0%
GATTAGATCAACGGTGAGTT+TGG - chr2_4:44830222-44830241 Msa0330440:CDS 40.0%
TGGTTATATATAGGGACGGA+GGG + chr2_4:44830489-44830508 None:intergenic 40.0%
AGGAAGGAATAGTGACTGTG+AGG + chr2_4:44829132-44829151 None:intergenic 45.0%
GTGTGGAATGTGGAATAGGA+AGG + chr2_4:44829148-44829167 None:intergenic 45.0%
TGGTGTGTGGAATGTGGAAT+AGG + chr2_4:44829152-44829171 None:intergenic 45.0%
GAAATGTGGTGTGTGGAATG+TGG + chr2_4:44829158-44829177 None:intergenic 45.0%
CAGAATCACTTCATCAGCCA+TGG + chr2_4:44829309-44829328 None:intergenic 45.0%
CAGAATAGCCCTAGCTGAAA+AGG - chr2_4:44829364-44829383 Msa0330440:three_prime_UTR 45.0%
! GCTTGATACCCTTTTCAGCT+AGG + chr2_4:44829376-44829395 None:intergenic 45.0%
AGAGATCACAAGCTAGGTTC+TGG - chr2_4:44829579-44829598 Msa0330440:CDS 45.0%
GAGATCACAAGCTAGGTTCT+GGG - chr2_4:44829580-44829599 Msa0330440:CDS 45.0%
ATGAAGGTGGAAGGAACCTT+TGG - chr2_4:44829863-44829882 Msa0330440:intron 45.0%
TGAAGGTGGAAGGAACCTTT+GGG - chr2_4:44829864-44829883 Msa0330440:intron 45.0%
AAGGAACCTTTGGGCCAAAA+AGG - chr2_4:44829873-44829892 Msa0330440:intron 45.0%
AGGAACCTTTGGGCCAAAAA+GGG - chr2_4:44829874-44829893 Msa0330440:intron 45.0%
TTCAAAGTCACTCCCTGATC+AGG - chr2_4:44830128-44830147 Msa0330440:CDS 45.0%
CATGGACCTTATCCTGATCA+GGG + chr2_4:44830143-44830162 None:intergenic 45.0%
TCATGGACCTTATCCTGATC+AGG + chr2_4:44830144-44830163 None:intergenic 45.0%
CATTGTAGAGCTGAGGAAGA+CGG - chr2_4:44830167-44830186 Msa0330440:CDS 45.0%
ATTGTAGAGCTGAGGAAGAC+GGG - chr2_4:44830168-44830187 Msa0330440:CDS 45.0%
!! AGGAAGACGGGTTATTGAGT+AGG - chr2_4:44830180-44830199 Msa0330440:CDS 45.0%
!! AGACGGGTTATTGAGTAGGT+TGG - chr2_4:44830184-44830203 Msa0330440:CDS 45.0%
GTGGTTATATATAGGGACGG+AGG + chr2_4:44830490-44830509 None:intergenic 45.0%
AGGAAGGAATAGTGACTGTG+AGG + chr2_4:44829132-44829151 None:intergenic 45.0%
GTGTGGAATGTGGAATAGGA+AGG + chr2_4:44829148-44829167 None:intergenic 45.0%
TGGTGTGTGGAATGTGGAAT+AGG + chr2_4:44829152-44829171 None:intergenic 45.0%
GAAATGTGGTGTGTGGAATG+TGG + chr2_4:44829158-44829177 None:intergenic 45.0%
CAGAATCACTTCATCAGCCA+TGG + chr2_4:44829309-44829328 None:intergenic 45.0%
CAGAATAGCCCTAGCTGAAA+AGG - chr2_4:44829364-44829383 Msa0330440:three_prime_UTR 45.0%
! GCTTGATACCCTTTTCAGCT+AGG + chr2_4:44829376-44829395 None:intergenic 45.0%
AGAGATCACAAGCTAGGTTC+TGG - chr2_4:44829579-44829598 Msa0330440:CDS 45.0%
GAGATCACAAGCTAGGTTCT+GGG - chr2_4:44829580-44829599 Msa0330440:CDS 45.0%
ATGAAGGTGGAAGGAACCTT+TGG - chr2_4:44829863-44829882 Msa0330440:intron 45.0%
TGAAGGTGGAAGGAACCTTT+GGG - chr2_4:44829864-44829883 Msa0330440:intron 45.0%
AAGGAACCTTTGGGCCAAAA+AGG - chr2_4:44829873-44829892 Msa0330440:intron 45.0%
AGGAACCTTTGGGCCAAAAA+GGG - chr2_4:44829874-44829893 Msa0330440:intron 45.0%
TTCAAAGTCACTCCCTGATC+AGG - chr2_4:44830128-44830147 Msa0330440:CDS 45.0%
CATGGACCTTATCCTGATCA+GGG + chr2_4:44830143-44830162 None:intergenic 45.0%
TCATGGACCTTATCCTGATC+AGG + chr2_4:44830144-44830163 None:intergenic 45.0%
CATTGTAGAGCTGAGGAAGA+CGG - chr2_4:44830167-44830186 Msa0330440:CDS 45.0%
ATTGTAGAGCTGAGGAAGAC+GGG - chr2_4:44830168-44830187 Msa0330440:CDS 45.0%
!! AGGAAGACGGGTTATTGAGT+AGG - chr2_4:44830180-44830199 Msa0330440:CDS 45.0%
!! AGACGGGTTATTGAGTAGGT+TGG - chr2_4:44830184-44830203 Msa0330440:CDS 45.0%
GTGGTTATATATAGGGACGG+AGG + chr2_4:44830490-44830509 None:intergenic 45.0%
!! TATTATATTATGTAATAAAA+AGG - chr2_4:44830374-44830393 Msa0330440:five_prime_UTR 5.0%
!! TATTATATTATGTAATAAAA+AGG - chr2_4:44830374-44830393 Msa0330440:five_prime_UTR 5.0%
GATTCCTGGGCAAGTCCATT+TGG - chr2_4:44829332-44829351 Msa0330440:three_prime_UTR 50.0%
!! CTTCTCCCTTTTTGGCCCAA+AGG + chr2_4:44829882-44829901 None:intergenic 50.0%
GTCACTCCCTGATCAGGATA+AGG - chr2_4:44830134-44830153 Msa0330440:CDS 50.0%
GATTCCTGGGCAAGTCCATT+TGG - chr2_4:44829332-44829351 Msa0330440:three_prime_UTR 50.0%
!! CTTCTCCCTTTTTGGCCCAA+AGG + chr2_4:44829882-44829901 None:intergenic 50.0%
GTCACTCCCTGATCAGGATA+AGG - chr2_4:44830134-44830153 Msa0330440:CDS 50.0%
! CATGCCAAATGGACTTGCCC+AGG + chr2_4:44829339-44829358 None:intergenic 55.0%
! CATGCCAAATGGACTTGCCC+AGG + chr2_4:44829339-44829358 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 44829093 44830539 44829093 ID=Msa0330440;Name=Msa0330440
chr2_4 mRNA 44829093 44830539 44829093 ID=Msa0330440-mRNA-1;Parent=Msa0330440;Name=Msa0330440-mRNA-1;_AED=0.02;_eAED=0.02;_QI=215|1|1|1|1|1|2|342|217
chr2_4 exon 44829093 44829773 44829093 ID=Msa0330440-mRNA-1:exon:13692;Parent=Msa0330440-mRNA-1
chr2_4 exon 44830010 44830539 44830010 ID=Msa0330440-mRNA-1:exon:13691;Parent=Msa0330440-mRNA-1
chr2_4 five_prime_UTR 44830325 44830539 44830325 ID=Msa0330440-mRNA-1:five_prime_utr;Parent=Msa0330440-mRNA-1
chr2_4 CDS 44830010 44830324 44830010 ID=Msa0330440-mRNA-1:cds;Parent=Msa0330440-mRNA-1
chr2_4 CDS 44829435 44829773 44829435 ID=Msa0330440-mRNA-1:cds;Parent=Msa0330440-mRNA-1
chr2_4 three_prime_UTR 44829093 44829434 44829093 ID=Msa0330440-mRNA-1:three_prime_utr;Parent=Msa0330440-mRNA-1
Gene Sequence

>Msa0330440

ATGGCTGATGAAGTGATTCTGCTTGATTCCTGGGCAAGTCCATTTGGCATGAGACTCAGAATAGCCCTAGCTGAAAAGGGTATCAAGCATGAGTACAAAGAAGAAGATCTAAGTAACAAAAGCCCTTTGTTGTTACAAATGAACCCTGTTCACAAGAAAATCCCTGTTCTGATTCATAACGGTAAACCTATTTGTGAATCTTTAGTTGCTGTTCAGTATATTGATGAGGTTTGGAATGAGAAATCTCCTTTGTTGCCTTCTGATCCTTATCAGAGATCACAAGCTAGGTTCTGGGCTGATTATGTTGATAAGAAGATCTATGAAGGTGGAAGGAACCTTTGGGCCAAAAAGGGAGAAGAGCAAGAAGCTGCCAAGAAAGAATTCATAGATGTCCTCAAATTGTTGGAGCAAGAATTGGGAGACAAGACTTATTTTGGAGGAGATAAGCTTGGTTTTGTTGATGTAGCACTTATTCCATTCTACACTTGGTTTAAAAGCTATGAGACCTTTGGAAACATCAATTTAGAGAAGGAATGCCCTAAGTTCATTGCTTGGTCCAAGAGATGTATGCAGATTGAGAGTGTTTCAAAGTCACTCCCTGATCAGGATAAGGTCCATGACTACATTGTAGAGCTGAGGAAGACGGGTTATTGA
Protein sequence

>Msa0330440

MADEVILLDSWASPFGMRLRIALAEKGIKHEYKEEDLSNKSPLLLQMNPVHKKIPVLIHNGKPICESLVAVQYIDEVWNEKSPLLPSDPYQRSQARFWADYVDKKIYEGGRNLWAKKGEEQEAAKKEFIDVLKLLEQELGDKTYFGGDKLGFVDVALIPFYTWFKSYETFGNINLEKECPKFIAWSKRCMQIESVSKSLPDQDKVHDYIVELRKTGY*