AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0334970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0334970 MtrunA17_Chr2g0319291 79.508 122 2 1 9 107 1 122 1.46e-64 191
Msa0334970 MtrunA17_Chr4g0042051 72.826 92 25 0 16 107 31 122 2.17e-47 147
Msa0334970 MtrunA17_Chr2g0280411 61.053 95 37 0 11 105 25 119 5.02e-40 129
Msa0334970 MtrunA17_Chr7g0216481 45.570 79 43 0 20 98 51 129 2.88e-22 84.3
Msa0334970 MtrunA17_Chr5g0404831 41.860 86 50 0 20 105 33 118 8.04e-22 82.8
Msa0334970 MtrunA17_Chr8g0334481 43.038 79 45 0 20 98 51 129 1.92e-21 82.4
Msa0334970 MtrunA17_Chr7g0245141 40.217 92 54 1 16 106 44 135 2.71e-21 82.0
Msa0334970 MtrunA17_Chr1g0155721 40.698 86 51 0 20 105 31 116 1.19e-19 77.4
Msa0334970 MtrunA17_Chr3g0142051 43.038 79 45 0 20 98 31 109 4.99e-19 75.9
Msa0334970 MtrunA17_Chr8g0393201 35.443 79 51 0 20 98 252 330 9.54e-18 76.3
Msa0334970 MtrunA17_Chr5g0415771 39.362 94 56 1 6 99 31 123 4.23e-16 68.6
Msa0334970 MtrunA17_Chr5g0415761 38.554 83 51 0 17 99 42 124 7.44e-13 60.1
Msa0334970 MtrunA17_Chr5g0415741 37.349 83 52 0 17 99 40 122 2.70e-12 58.5
Msa0334970 MtrunA17_Chr4g0047431 32.967 91 61 0 17 107 21 111 4.40e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0334970 AT1G11530.1 64.444 90 32 0 16 105 23 112 2.13e-42 135
Msa0334970 AT3G08710.2 45.977 87 47 0 20 106 48 134 1.57e-22 85.9
Msa0334970 AT3G08710.3 45.977 87 47 0 20 106 48 134 1.57e-22 85.9
Msa0334970 AT3G08710.1 45.977 87 47 0 20 106 48 134 1.57e-22 85.9
Msa0334970 AT3G51030.1 48.101 79 41 0 20 98 31 109 3.37e-22 84.0
Msa0334970 AT3G56420.1 43.038 79 45 0 20 98 65 143 2.98e-20 80.1
Msa0334970 AT3G56420.2 43.038 79 45 0 20 98 65 143 2.98e-20 80.1
Msa0334970 AT3G56420.3 43.038 79 45 0 20 98 65 143 2.98e-20 80.1
Msa0334970 AT1G45145.1 37.975 79 49 0 20 98 30 108 1.18e-19 77.8
Msa0334970 AT2G40790.2 41.772 79 46 0 20 98 65 143 1.44e-19 78.6
Msa0334970 AT2G40790.1 41.772 79 46 0 20 98 65 143 1.44e-19 78.6
Msa0334970 AT1G59730.1 34.694 98 63 1 2 99 29 125 1.05e-18 75.5
Msa0334970 AT1G19730.1 36.145 83 52 1 17 98 28 110 5.05e-18 73.6
Msa0334970 AT5G39950.1 39.024 82 50 0 17 98 47 128 5.79e-18 73.9
Msa0334970 AT3G17880.2 34.177 79 52 0 20 98 288 366 9.91e-17 74.3
Msa0334970 AT3G17880.1 34.177 79 52 0 20 98 295 373 1.04e-16 74.3
Msa0334970 AT5G42980.1 38.667 75 46 0 20 94 30 104 1.60e-16 69.7
Msa0334970 AT1G69880.1 32.955 88 59 0 17 104 59 146 1.19e-15 68.2
Msa0334970 AT4G04950.1 32.967 91 61 0 17 107 21 111 5.99e-13 63.9
Msa0334970 AT4G32580.1 29.808 104 71 2 5 107 10 112 1.46e-12 60.5
Msa0334970 AT2G35010.2 32.530 83 54 1 21 101 110 192 5.67e-11 57.0
Msa0334970 AT2G35010.1 32.530 83 54 1 21 101 110 192 5.67e-11 57.0

Find 31 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCCATAATAGATCTTT+AGG 0.279018 2_4:+54075862 None:intergenic
TACTGGTTGTTGTGTGTGTT+TGG 0.281902 2_4:-54075778 Msa0334970:three_prime_UTR
TCCTTCCTAAAGATCTATTA+TGG 0.287061 2_4:-54075867 Msa0334970:exon
AAAGGATTCATTTGTATAGA+AGG 0.303846 2_4:+54076911 None:intergenic
GAATCCTTTCTTTCAAGAAT+TGG 0.317619 2_4:-54076897 Msa0334970:CDS
ATAGCCTAGAACTATTGTTT+TGG 0.322998 2_4:-54075804 Msa0334970:three_prime_UTR
AGGTCCAAAACAATAGTTCT+AGG 0.348972 2_4:+54075800 None:intergenic
TTGATAAAGTTATAAAAGAT+AGG 0.352325 2_4:+54075689 None:intergenic
TATGGCTTTAATTTCCATCT+TGG 0.365887 2_4:+54075991 None:intergenic
GATGCCAATTCTTGAAAGAA+AGG 0.374604 2_4:+54076893 None:intergenic
GAGTATTAAATGAGAAATAC+TGG 0.379960 2_4:+54075712 None:intergenic
GTGCAAATCCTGATGAAATA+AGG 0.381132 2_4:-54075919 Msa0334970:CDS
ACTGGTTGTTGTGTGTGTTT+GGG 0.390313 2_4:-54075777 Msa0334970:three_prime_UTR
ATTGGATGTGGATGAGGTTA+AGG 0.406643 2_4:-54076843 Msa0334970:intron
TTTCTTTGTGTAAATATTTG+TGG 0.422046 2_4:-54075487 Msa0334970:three_prime_UTR
GGCTTTAATTTCCATCTTGG+AGG 0.439612 2_4:+54075994 None:intergenic
ATCCTGATGAAATAAGGAAA+AGG 0.449385 2_4:-54075913 Msa0334970:CDS
ACAACAACAAAAGAAAGAAA+TGG 0.465941 2_4:-54077314 Msa0334970:CDS
TCTCACATTGGATGTGGATG+AGG 0.479212 2_4:-54076849 Msa0334970:CDS
TCCTGATGAAATAAGGAAAA+GGG 0.497571 2_4:-54075912 Msa0334970:CDS
ATGATGTTAGTGTACATAGA+TGG 0.509088 2_4:-54075563 Msa0334970:three_prime_UTR
TGATAAAGTTATAAAAGATA+GGG 0.526654 2_4:+54075690 None:intergenic
TCCATAATAGATCTTTAGGA+AGG 0.541604 2_4:+54075866 None:intergenic
TCAGGAAATTGCCTCCAAGA+TGG 0.545444 2_4:-54076005 Msa0334970:intron
AACTCTTGTGGACAAAATTG+TGG 0.565813 2_4:-54075942 Msa0334970:CDS
TTTGAATGGAGGAACTCTTG+TGG 0.568819 2_4:-54075954 Msa0334970:CDS
ACAACAAAAGAAAGAAATGG+AGG 0.594542 2_4:-54077311 Msa0334970:CDS
CAACAAAAGAAAGAAATGGA+GGG 0.610986 2_4:-54077310 Msa0334970:CDS
ACTCTTGTGGACAAAATTGT+GGG 0.620063 2_4:-54075941 Msa0334970:CDS
TGGAAACAATGAAATGAACA+AGG 0.675776 2_4:-54075847 Msa0334970:three_prime_UTR
AAACACACACAACAACCAGT+AGG 0.679842 2_4:+54075780 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTATAATAAATTTTTT+TGG + chr2_4:54076486-54076505 None:intergenic 0.0%
!!! TTTATTATAATAAATTTTTT+TGG + chr2_4:54076486-54076505 None:intergenic 0.0%
!!! CTTTTAAATATTACTAAAAT+TGG - chr2_4:54075793-54075812 Msa0334970:three_prime_UTR 10.0%
!! TAATTTATGATTTATGATAT+AGG + chr2_4:54076649-54076668 None:intergenic 10.0%
!!! AGTAGTTTTTTTTTTTTTTA+AGG + chr2_4:54077182-54077201 None:intergenic 10.0%
!!! CTTTTAAATATTACTAAAAT+TGG - chr2_4:54075793-54075812 Msa0334970:three_prime_UTR 10.0%
!! TAATTTATGATTTATGATAT+AGG + chr2_4:54076649-54076668 None:intergenic 10.0%
!!! AGTAGTTTTTTTTTTTTTTA+AGG + chr2_4:54077182-54077201 None:intergenic 10.0%
!!! TAGAATTTTTGAGATATTTT+TGG + chr2_4:54075491-54075510 None:intergenic 15.0%
!! AAAATATCTCAAAAATTCTA+AGG - chr2_4:54075491-54075510 Msa0334970:three_prime_UTR 15.0%
!!! AATTTGGAGTAAAAAATAAA+AGG + chr2_4:54076367-54076386 None:intergenic 15.0%
!! TTTGTAGGATAATAAATTAT+CGG - chr2_4:54076417-54076436 Msa0334970:intron 15.0%
!! TGATAAAGTTATAAAAGATA+GGG + chr2_4:54077088-54077107 None:intergenic 15.0%
!! TTGATAAAGTTATAAAAGAT+AGG + chr2_4:54077089-54077108 None:intergenic 15.0%
!!! ACTTTATCAAGTTTTTTTTT+AGG - chr2_4:54077099-54077118 Msa0334970:intron 15.0%
!!! CTTTATCAAGTTTTTTTTTA+GGG - chr2_4:54077100-54077119 Msa0334970:intron 15.0%
!!! AACTATATCATGTTTTTATT+TGG - chr2_4:54077123-54077142 Msa0334970:intron 15.0%
!!! TAGAATTTTTGAGATATTTT+TGG + chr2_4:54075491-54075510 None:intergenic 15.0%
!! AAAATATCTCAAAAATTCTA+AGG - chr2_4:54075491-54075510 Msa0334970:three_prime_UTR 15.0%
!!! AATTTGGAGTAAAAAATAAA+AGG + chr2_4:54076367-54076386 None:intergenic 15.0%
!! TTTGTAGGATAATAAATTAT+CGG - chr2_4:54076417-54076436 Msa0334970:intron 15.0%
!! TGATAAAGTTATAAAAGATA+GGG + chr2_4:54077088-54077107 None:intergenic 15.0%
!! TTGATAAAGTTATAAAAGAT+AGG + chr2_4:54077089-54077108 None:intergenic 15.0%
!!! ACTTTATCAAGTTTTTTTTT+AGG - chr2_4:54077099-54077118 Msa0334970:intron 15.0%
!!! CTTTATCAAGTTTTTTTTTA+GGG - chr2_4:54077100-54077119 Msa0334970:intron 15.0%
!!! AACTATATCATGTTTTTATT+TGG - chr2_4:54077123-54077142 Msa0334970:intron 15.0%
!! AAATAGACTCAAAAAAATCA+TGG - chr2_4:54075520-54075539 Msa0334970:three_prime_UTR 20.0%
!! AATAGACTCAAAAAAATCAT+GGG - chr2_4:54075521-54075540 Msa0334970:three_prime_UTR 20.0%
!!! AATGATTTTTCATAAAGGAA+AGG + chr2_4:54075593-54075612 None:intergenic 20.0%
!!! TGCTAAATGATTTTTCATAA+AGG + chr2_4:54075598-54075617 None:intergenic 20.0%
!! TGTGTACATTAATATATATG+TGG - chr2_4:54076741-54076760 Msa0334970:intron 20.0%
!!! TATATCATGTTTTTATTTGG+TGG - chr2_4:54077126-54077145 Msa0334970:intron 20.0%
!!! ATATCATGTTTTTATTTGGT+GGG - chr2_4:54077127-54077146 Msa0334970:intron 20.0%
!! TTTCTTTGTGTAAATATTTG+TGG - chr2_4:54077288-54077307 Msa0334970:CDS 20.0%
!! AAATAGACTCAAAAAAATCA+TGG - chr2_4:54075520-54075539 Msa0334970:three_prime_UTR 20.0%
!! AATAGACTCAAAAAAATCAT+GGG - chr2_4:54075521-54075540 Msa0334970:three_prime_UTR 20.0%
!!! AATGATTTTTCATAAAGGAA+AGG + chr2_4:54075593-54075612 None:intergenic 20.0%
!!! TGCTAAATGATTTTTCATAA+AGG + chr2_4:54075598-54075617 None:intergenic 20.0%
!! TGTGTACATTAATATATATG+TGG - chr2_4:54076741-54076760 Msa0334970:intron 20.0%
!!! TATATCATGTTTTTATTTGG+TGG - chr2_4:54077126-54077145 Msa0334970:intron 20.0%
!!! ATATCATGTTTTTATTTGGT+GGG - chr2_4:54077127-54077146 Msa0334970:intron 20.0%
!! TTTCTTTGTGTAAATATTTG+TGG - chr2_4:54077288-54077307 Msa0334970:CDS 20.0%
! ACAACAACAAAAGAAAGAAA+TGG - chr2_4:54075461-54075480 Msa0334970:three_prime_UTR 25.0%
!!! CTCACTGTAATTTTAATATG+CGG + chr2_4:54075623-54075642 None:intergenic 25.0%
! AAAGGATTCATTTGTATAGA+AGG + chr2_4:54075867-54075886 None:intergenic 25.0%
!! ATGTGCTTAACTTTTAACAA+TGG - chr2_4:54076224-54076243 Msa0334970:intron 25.0%
! AAGCATGAACTCATTTAATT+TGG + chr2_4:54076383-54076402 None:intergenic 25.0%
! TGAACCTTATTATAACGTTA+GGG - chr2_4:54076601-54076620 Msa0334970:intron 25.0%
!! TTTTCCCTAACGTTATAATA+AGG + chr2_4:54076608-54076627 None:intergenic 25.0%
! GTACACAATAATAGAAAAAG+TGG + chr2_4:54076728-54076747 None:intergenic 25.0%
! TGTTTCCATAATAGATCTTT+AGG + chr2_4:54076916-54076935 None:intergenic 25.0%
! GAGTATTAAATGAGAAATAC+TGG + chr2_4:54077066-54077085 None:intergenic 25.0%
! ACAACAACAAAAGAAAGAAA+TGG - chr2_4:54075461-54075480 Msa0334970:three_prime_UTR 25.0%
!!! CTCACTGTAATTTTAATATG+CGG + chr2_4:54075623-54075642 None:intergenic 25.0%
! AAAGGATTCATTTGTATAGA+AGG + chr2_4:54075867-54075886 None:intergenic 25.0%
!! ATGTGCTTAACTTTTAACAA+TGG - chr2_4:54076224-54076243 Msa0334970:intron 25.0%
! AAGCATGAACTCATTTAATT+TGG + chr2_4:54076383-54076402 None:intergenic 25.0%
! TGAACCTTATTATAACGTTA+GGG - chr2_4:54076601-54076620 Msa0334970:intron 25.0%
!! TTTTCCCTAACGTTATAATA+AGG + chr2_4:54076608-54076627 None:intergenic 25.0%
! GTACACAATAATAGAAAAAG+TGG + chr2_4:54076728-54076747 None:intergenic 25.0%
! TGTTTCCATAATAGATCTTT+AGG + chr2_4:54076916-54076935 None:intergenic 25.0%
! GAGTATTAAATGAGAAATAC+TGG + chr2_4:54077066-54077085 None:intergenic 25.0%
ACAACAAAAGAAAGAAATGG+AGG - chr2_4:54075464-54075483 Msa0334970:three_prime_UTR 30.0%
CAACAAAAGAAAGAAATGGA+GGG - chr2_4:54075465-54075484 Msa0334970:three_prime_UTR 30.0%
!! AAGTTTTGTCAAAATCACAG+TGG - chr2_4:54075699-54075718 Msa0334970:three_prime_UTR 30.0%
! AAATTGGTCTAAAATACTGC+AGG - chr2_4:54075809-54075828 Msa0334970:three_prime_UTR 30.0%
GAATCCTTTCTTTCAAGAAT+TGG - chr2_4:54075878-54075897 Msa0334970:exon 30.0%
! AGATGTTCTTTTTCTCACAT+TGG - chr2_4:54075914-54075933 Msa0334970:CDS 30.0%
! ACACTTTAGATGGATTTGTA+TGG + chr2_4:54076087-54076106 None:intergenic 30.0%
! CCATCTAAAGTGTTTGTATT+CGG - chr2_4:54076094-54076113 Msa0334970:intron 30.0%
ATATGATACATAGTGGTGTT+TGG + chr2_4:54076123-54076142 None:intergenic 30.0%
GCATATAGTATAAGCAAACA+AGG + chr2_4:54076149-54076168 None:intergenic 30.0%
! TCCAACTATTGGTATTTTGT+AGG - chr2_4:54076402-54076421 Msa0334970:intron 30.0%
TCCTACAAAATACCAATAGT+TGG + chr2_4:54076406-54076425 None:intergenic 30.0%
GTGAACCTTATTATAACGTT+AGG - chr2_4:54076600-54076619 Msa0334970:intron 30.0%
ATATATATGTGGCTACATTC+AGG - chr2_4:54076752-54076771 Msa0334970:intron 30.0%
!! TATGGCTTTAATTTCCATCT+TGG + chr2_4:54076787-54076806 None:intergenic 30.0%
CAAAAAAAGAAAGGTAGGTA+TGG + chr2_4:54076805-54076824 None:intergenic 30.0%
CCATTCAAAAAAAGAAAGGT+AGG + chr2_4:54076810-54076829 None:intergenic 30.0%
!!! CCTACCTTTCTTTTTTTGAA+TGG - chr2_4:54076807-54076826 Msa0334970:intron 30.0%
!!! ACCTTTCTTTTTTTGAATGG+AGG - chr2_4:54076810-54076829 Msa0334970:intron 30.0%
TCCTCCATTCAAAAAAAGAA+AGG + chr2_4:54076814-54076833 None:intergenic 30.0%
ATCCTGATGAAATAAGGAAA+AGG - chr2_4:54076862-54076881 Msa0334970:CDS 30.0%
TCCTGATGAAATAAGGAAAA+GGG - chr2_4:54076863-54076882 Msa0334970:CDS 30.0%
TCCTTCCTAAAGATCTATTA+TGG - chr2_4:54076908-54076927 Msa0334970:CDS 30.0%
! TCCATAATAGATCTTTAGGA+AGG + chr2_4:54076912-54076931 None:intergenic 30.0%
TGGAAACAATGAAATGAACA+AGG - chr2_4:54076928-54076947 Msa0334970:CDS 30.0%
! ATAGCCTAGAACTATTGTTT+TGG - chr2_4:54076971-54076990 Msa0334970:intron 30.0%
ATGATGTTAGTGTACATAGA+TGG - chr2_4:54077212-54077231 Msa0334970:intron 30.0%
ACAACAAAAGAAAGAAATGG+AGG - chr2_4:54075464-54075483 Msa0334970:three_prime_UTR 30.0%
CAACAAAAGAAAGAAATGGA+GGG - chr2_4:54075465-54075484 Msa0334970:three_prime_UTR 30.0%
!! AAGTTTTGTCAAAATCACAG+TGG - chr2_4:54075699-54075718 Msa0334970:three_prime_UTR 30.0%
! AAATTGGTCTAAAATACTGC+AGG - chr2_4:54075809-54075828 Msa0334970:three_prime_UTR 30.0%
GAATCCTTTCTTTCAAGAAT+TGG - chr2_4:54075878-54075897 Msa0334970:exon 30.0%
! AGATGTTCTTTTTCTCACAT+TGG - chr2_4:54075914-54075933 Msa0334970:CDS 30.0%
! ACACTTTAGATGGATTTGTA+TGG + chr2_4:54076087-54076106 None:intergenic 30.0%
! CCATCTAAAGTGTTTGTATT+CGG - chr2_4:54076094-54076113 Msa0334970:intron 30.0%
ATATGATACATAGTGGTGTT+TGG + chr2_4:54076123-54076142 None:intergenic 30.0%
GCATATAGTATAAGCAAACA+AGG + chr2_4:54076149-54076168 None:intergenic 30.0%
! TCCAACTATTGGTATTTTGT+AGG - chr2_4:54076402-54076421 Msa0334970:intron 30.0%
TCCTACAAAATACCAATAGT+TGG + chr2_4:54076406-54076425 None:intergenic 30.0%
GTGAACCTTATTATAACGTT+AGG - chr2_4:54076600-54076619 Msa0334970:intron 30.0%
ATATATATGTGGCTACATTC+AGG - chr2_4:54076752-54076771 Msa0334970:intron 30.0%
!! TATGGCTTTAATTTCCATCT+TGG + chr2_4:54076787-54076806 None:intergenic 30.0%
CAAAAAAAGAAAGGTAGGTA+TGG + chr2_4:54076805-54076824 None:intergenic 30.0%
CCATTCAAAAAAAGAAAGGT+AGG + chr2_4:54076810-54076829 None:intergenic 30.0%
!!! CCTACCTTTCTTTTTTTGAA+TGG - chr2_4:54076807-54076826 Msa0334970:intron 30.0%
!!! ACCTTTCTTTTTTTGAATGG+AGG - chr2_4:54076810-54076829 Msa0334970:intron 30.0%
TCCTCCATTCAAAAAAAGAA+AGG + chr2_4:54076814-54076833 None:intergenic 30.0%
ATCCTGATGAAATAAGGAAA+AGG - chr2_4:54076862-54076881 Msa0334970:CDS 30.0%
TCCTGATGAAATAAGGAAAA+GGG - chr2_4:54076863-54076882 Msa0334970:CDS 30.0%
TCCTTCCTAAAGATCTATTA+TGG - chr2_4:54076908-54076927 Msa0334970:CDS 30.0%
! TCCATAATAGATCTTTAGGA+AGG + chr2_4:54076912-54076931 None:intergenic 30.0%
TGGAAACAATGAAATGAACA+AGG - chr2_4:54076928-54076947 Msa0334970:CDS 30.0%
! ATAGCCTAGAACTATTGTTT+TGG - chr2_4:54076971-54076990 Msa0334970:intron 30.0%
ATGATGTTAGTGTACATAGA+TGG - chr2_4:54077212-54077231 Msa0334970:intron 30.0%
ACTTACTGGGTAACTTTGAT+TGG + chr2_4:54075568-54075587 None:intergenic 35.0%
TAAAGGAAAGGAGACTTACT+GGG + chr2_4:54075581-54075600 None:intergenic 35.0%
ATAAAGGAAAGGAGACTTAC+TGG + chr2_4:54075582-54075601 None:intergenic 35.0%
!! TGGTTCATTTCTCTGCTTTT+TGG - chr2_4:54075835-54075854 Msa0334970:three_prime_UTR 35.0%
GATGCCAATTCTTGAAAGAA+AGG + chr2_4:54075885-54075904 None:intergenic 35.0%
! TCTTTTTCTCACATTGGATG+TGG - chr2_4:54075920-54075939 Msa0334970:CDS 35.0%
CATTTGCATGTCTTAGCAAA+TGG + chr2_4:54076035-54076054 None:intergenic 35.0%
! CATTTGCTAAGACATGCAAA+TGG - chr2_4:54076033-54076052 Msa0334970:intron 35.0%
GACATGCAAATGGTATCTAA+AGG - chr2_4:54076043-54076062 Msa0334970:intron 35.0%
CCGAATACAAACACTTTAGA+TGG + chr2_4:54076097-54076116 None:intergenic 35.0%
! AAGGAGCATATGATACATAG+TGG + chr2_4:54076130-54076149 None:intergenic 35.0%
GATCTAGAAACTGAAATTGC+AGG + chr2_4:54076336-54076355 None:intergenic 35.0%
AACTCTTGTGGACAAAATTG+TGG - chr2_4:54076833-54076852 Msa0334970:intron 35.0%
ACTCTTGTGGACAAAATTGT+GGG - chr2_4:54076834-54076853 Msa0334970:intron 35.0%
GTGCAAATCCTGATGAAATA+AGG - chr2_4:54076856-54076875 Msa0334970:CDS 35.0%
!! ACCCTTTTCCTTATTTCATC+AGG + chr2_4:54076867-54076886 None:intergenic 35.0%
AGGTCCAAAACAATAGTTCT+AGG + chr2_4:54076978-54076997 None:intergenic 35.0%
!!! AACTATTGTTTTGGACCTAC+TGG - chr2_4:54076980-54076999 Msa0334970:intron 35.0%
ACTTACTGGGTAACTTTGAT+TGG + chr2_4:54075568-54075587 None:intergenic 35.0%
TAAAGGAAAGGAGACTTACT+GGG + chr2_4:54075581-54075600 None:intergenic 35.0%
ATAAAGGAAAGGAGACTTAC+TGG + chr2_4:54075582-54075601 None:intergenic 35.0%
!! TGGTTCATTTCTCTGCTTTT+TGG - chr2_4:54075835-54075854 Msa0334970:three_prime_UTR 35.0%
GATGCCAATTCTTGAAAGAA+AGG + chr2_4:54075885-54075904 None:intergenic 35.0%
! TCTTTTTCTCACATTGGATG+TGG - chr2_4:54075920-54075939 Msa0334970:CDS 35.0%
CATTTGCATGTCTTAGCAAA+TGG + chr2_4:54076035-54076054 None:intergenic 35.0%
! CATTTGCTAAGACATGCAAA+TGG - chr2_4:54076033-54076052 Msa0334970:intron 35.0%
GACATGCAAATGGTATCTAA+AGG - chr2_4:54076043-54076062 Msa0334970:intron 35.0%
CCGAATACAAACACTTTAGA+TGG + chr2_4:54076097-54076116 None:intergenic 35.0%
! AAGGAGCATATGATACATAG+TGG + chr2_4:54076130-54076149 None:intergenic 35.0%
GATCTAGAAACTGAAATTGC+AGG + chr2_4:54076336-54076355 None:intergenic 35.0%
AACTCTTGTGGACAAAATTG+TGG - chr2_4:54076833-54076852 Msa0334970:intron 35.0%
ACTCTTGTGGACAAAATTGT+GGG - chr2_4:54076834-54076853 Msa0334970:intron 35.0%
GTGCAAATCCTGATGAAATA+AGG - chr2_4:54076856-54076875 Msa0334970:CDS 35.0%
!! ACCCTTTTCCTTATTTCATC+AGG + chr2_4:54076867-54076886 None:intergenic 35.0%
AGGTCCAAAACAATAGTTCT+AGG + chr2_4:54076978-54076997 None:intergenic 35.0%
!!! AACTATTGTTTTGGACCTAC+TGG - chr2_4:54076980-54076999 Msa0334970:intron 35.0%
! GTCTAAAATACTGCAGGTTG+TGG - chr2_4:54075815-54075834 Msa0334970:three_prime_UTR 40.0%
ATTGGATGTGGATGAGGTTA+AGG - chr2_4:54075932-54075951 Msa0334970:CDS 40.0%
GAGTTCATGCTTCCAACTAT+TGG - chr2_4:54076391-54076410 Msa0334970:intron 40.0%
! GGCTTTAATTTCCATCTTGG+AGG + chr2_4:54076784-54076803 None:intergenic 40.0%
TTTGAATGGAGGAACTCTTG+TGG - chr2_4:54076821-54076840 Msa0334970:intron 40.0%
AAACACACACAACAACCAGT+AGG + chr2_4:54076998-54077017 None:intergenic 40.0%
! TACTGGTTGTTGTGTGTGTT+TGG - chr2_4:54076997-54077016 Msa0334970:intron 40.0%
ACTGGTTGTTGTGTGTGTTT+GGG - chr2_4:54076998-54077017 Msa0334970:intron 40.0%
! GTCTAAAATACTGCAGGTTG+TGG - chr2_4:54075815-54075834 Msa0334970:three_prime_UTR 40.0%
ATTGGATGTGGATGAGGTTA+AGG - chr2_4:54075932-54075951 Msa0334970:CDS 40.0%
GAGTTCATGCTTCCAACTAT+TGG - chr2_4:54076391-54076410 Msa0334970:intron 40.0%
! GGCTTTAATTTCCATCTTGG+AGG + chr2_4:54076784-54076803 None:intergenic 40.0%
TTTGAATGGAGGAACTCTTG+TGG - chr2_4:54076821-54076840 Msa0334970:intron 40.0%
AAACACACACAACAACCAGT+AGG + chr2_4:54076998-54077017 None:intergenic 40.0%
! TACTGGTTGTTGTGTGTGTT+TGG - chr2_4:54076997-54077016 Msa0334970:intron 40.0%
ACTGGTTGTTGTGTGTGTTT+GGG - chr2_4:54076998-54077017 Msa0334970:intron 40.0%
TCTCACATTGGATGTGGATG+AGG - chr2_4:54075926-54075945 Msa0334970:CDS 45.0%
TCAGGAAATTGCCTCCAAGA+TGG - chr2_4:54076770-54076789 Msa0334970:intron 45.0%
TCTCACATTGGATGTGGATG+AGG - chr2_4:54075926-54075945 Msa0334970:CDS 45.0%
TCAGGAAATTGCCTCCAAGA+TGG - chr2_4:54076770-54076789 Msa0334970:intron 45.0%
!!! TATAATAAATTATTCATTTT+TGG - chr2_4:54076497-54076516 Msa0334970:intron 5.0%
!!! TATAATAAATTATTCATTTT+TGG - chr2_4:54076497-54076516 Msa0334970:intron 5.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 54075456 54077341 54075456 ID=Msa0334970;Name=Msa0334970
chr2_4 mRNA 54075456 54077341 54075456 ID=Msa0334970-mRNA-1;Parent=Msa0334970;Name=Msa0334970-mRNA-1;_AED=0.13;_eAED=0.16;_QI=0|0.5|0.66|1|1|1|3|424|107
chr2_4 exon 54075456 54076023 54075456 ID=Msa0334970-mRNA-1:exon:16292;Parent=Msa0334970-mRNA-1
chr2_4 exon 54076844 54076966 54076844 ID=Msa0334970-mRNA-1:exon:16291;Parent=Msa0334970-mRNA-1
chr2_4 exon 54077285 54077341 54077285 ID=Msa0334970-mRNA-1:exon:16290;Parent=Msa0334970-mRNA-1
chr2_4 CDS 54077285 54077341 54077285 ID=Msa0334970-mRNA-1:cds;Parent=Msa0334970-mRNA-1
chr2_4 CDS 54076844 54076966 54076844 ID=Msa0334970-mRNA-1:cds;Parent=Msa0334970-mRNA-1
chr2_4 CDS 54075880 54076023 54075880 ID=Msa0334970-mRNA-1:cds;Parent=Msa0334970-mRNA-1
chr2_4 three_prime_UTR 54075456 54075879 54075456 ID=Msa0334970-mRNA-1:three_prime_utr;Parent=Msa0334970-mRNA-1
Gene Sequence

>Msa0334970

ATGCGACAACAACAAAAGAAAGAAATGGAGGGCCAAAAATATCTCAAAAATTCTAAGGTTGTGGTTCATTTCTCTGCTTTTTGGTGTGTTCCTTCTATACAAATGAATCCTTTCTTTCAAGAATTGGCATCAAACTATCAAGATGTTCTTTTTCTCACATTGGATGTGGATGAGGTTAAGGAAATTGCCTCCAAGATGGAAATTAAAGCCATACCTACCTTTCTTTTTTTGAATGGAGGAACTCTTGTGGACAAAATTGTGGGTGCAAATCCTGATGAAATAAGGAAAAGGGTTGATCATTTTATTCAATCAACTCCTTCCTAA
Protein sequence

>Msa0334970

MRQQQKKEMEGQKYLKNSKVVVHFSAFWCVPSIQMNPFFQELASNYQDVLFLTLDVDEVKEIASKMEIKAIPTFLFLNGGTLVDKIVGANPDEIRKRVDHFIQSTPS*