AlfalfaGEDB | Msa0336210

M. sativa cultivar zhongmu-4 / Msa0336210

KEGG: K08857
GO: GO:0000226 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0008152 GO:0008544 GO:0009888 GO:0009913 GO:0009987 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030154 GO:0030855 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048856 GO:0048869 GO:0055028 GO:0060429 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336210	XP_003596869.3	92.124	1003	71	2	5	1000	1	1002	0.0	1819

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336210	sp\|Q0WPH8\|NEK5_ARATH	50.593	1012	413	28	1	995	1	942	0.0	812

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0336210	Msa1463710	0.958216	4.076221e-116	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336210	MtrunA17_Chr2g0321841	92.124	1003	71	2	5	1000	1	1002	0.0	1819
Msa0336210	MtrunA17_Chr4g0025671	59.419	1067	324	17	1	999	1	1026	0.0	1081
Msa0336210	MtrunA17_Chr1g0190831	70.956	272	79	0	5	276	1	272	6.25e-143	440
Msa0336210	MtrunA17_Chr1g0190831	65.079	63	22	0	928	990	546	608	7.06e-17	85.5
Msa0336210	MtrunA17_Chr7g0258051	62.048	332	117	3	4	332	12	337	1.42e-141	437
Msa0336210	MtrunA17_Chr7g0258051	78.000	50	11	0	939	988	583	632	5.37e-15	79.7
Msa0336210	MtrunA17_Chr7g0260191	68.478	276	87	0	5	280	1	276	4.79e-141	436
Msa0336210	MtrunA17_Chr7g0260191	68.966	58	18	0	932	989	553	610	2.09e-16	84.0
Msa0336210	MtrunA17_Chr1g0185221	62.838	296	109	1	5	299	1	296	1.39e-137	426
Msa0336210	MtrunA17_Chr1g0185221	64.615	65	23	0	929	993	537	601	1.42e-16	84.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0336210	AT3G44200.2	50.593	1012	413	28	1	995	1	942	0.0	812
Msa0336210	AT3G44200.1	50.593	1012	413	28	1	995	1	942	0.0	812
Msa0336210	AT1G54510.4	71.756	262	74	0	5	266	1	262	2.84e-139	431
Msa0336210	AT1G54510.4	72.414	58	16	0	932	989	532	589	6.81e-17	85.9
Msa0336210	AT1G54510.2	71.756	262	74	0	5	266	1	262	2.84e-139	431
Msa0336210	AT1G54510.2	72.414	58	16	0	932	989	532	589	6.81e-17	85.9
Msa0336210	AT1G54510.1	71.756	262	74	0	5	266	1	262	2.84e-139	431
Msa0336210	AT1G54510.1	72.414	58	16	0	932	989	532	589	6.81e-17	85.9
Msa0336210	AT1G54510.3	71.756	262	74	0	5	266	1	262	2.84e-139	431
Msa0336210	AT1G54510.3	72.414	58	16	0	932	989	532	589	6.81e-17	85.9

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Find 247 sgRNAs with CRISPR-Local

Find 579 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGTAAGGTCTCCTCAA+GGG	0.624519	2_4:-55531438	Msa0336210:CDS
AAAAGTTGGTGTAAACGGAG+AGG	0.671095	2_4:+55532423	None:intergenic
AAACAGATAAACAGGAGGTT+AGG	0.444849	2_4:-55533360	Msa0336210:CDS
AAACATTCACTTTCCATTTC+TGG	0.153915	2_4:-55532205	Msa0336210:CDS
AAAGGCTCTCTAGAAACATG+TGG	0.658063	2_4:+55532664	None:intergenic
AAAGTAGAAACTTACAAGGA+TGG	0.543494	2_4:+55534193	None:intergenic
AAAGTTGGCACCTCTACATT+AGG	0.381919	2_4:+55533121	None:intergenic
AAATATTTGTAACAGGTTGT+AGG	0.401428	2_4:-55535781	Msa0336210:intron
AAATCAGATTTCGGTGTTAA+TGG	0.223289	2_4:-55533151	Msa0336210:CDS
AAATCTGATATTTGGTCCCT+AGG	0.465642	2_4:-55535706	Msa0336210:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGTTTATTTATGGTTAGAG+GGG	+	chr2_4:55531119-55531138	None:intergenic	30.0%
AGAGGGAAATGGTTTATTTA+TGG	+	chr2_4:55531128-55531147	None:intergenic	30.0%
ACGTTCATTCATTTCTCAAA+CGG	-	chr2_4:55531347-55531366	Msa0336210:three_prime_UTR	30.0%
ATTTGCAATGATGTCTAAGA+TGG	-	chr2_4:55531460-55531479	Msa0336210:CDS	30.0%
CTTCTGATCAAATGTGTATT+TGG	-	chr2_4:55531778-55531797	Msa0336210:CDS	30.0%
TTGGGAATTGTATTTGTAGA+TGG	-	chr2_4:55532010-55532029	Msa0336210:CDS	30.0%
TCCTTACATTGTTGAATTCA+AGG	-	chr2_4:55532055-55532074	Msa0336210:CDS	30.0%
TCCTTGAATTCAACAATGTA+AGG	+	chr2_4:55532059-55532078	None:intergenic	30.0%
TAAAAAGAACTAGTTGTTGG+AGG	+	chr2_4:55532362-55532381	None:intergenic	30.0%
AAACACAGAATATGAAACAG+AGG	-	chr2_4:55532536-55532555	Msa0336210:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	55531086	55539454	55531086	ID=Msa0336210;Name=Msa0336210
chr2_4	mRNA	55531086	55539454	55531086	ID=Msa0336210-mRNA-1;Parent=Msa0336210;Name=Msa0336210-mRNA-1;_AED=0.07;_eAED=0.07;_QI=381\|0.92\|0.92\|1\|1\|1\|14\|284\|1000
chr2_4	exon	55531086	55532965	55531086	ID=Msa0336210-mRNA-1:exon:16847;Parent=Msa0336210-mRNA-1
chr2_4	exon	55533054	55533704	55533054	ID=Msa0336210-mRNA-1:exon:16846;Parent=Msa0336210-mRNA-1
chr2_4	exon	55533798	55533849	55533798	ID=Msa0336210-mRNA-1:exon:16845;Parent=Msa0336210-mRNA-1
chr2_4	exon	55534208	55534281	55534208	ID=Msa0336210-mRNA-1:exon:16844;Parent=Msa0336210-mRNA-1
chr2_4	exon	55535584	55535633	55535584	ID=Msa0336210-mRNA-1:exon:16843;Parent=Msa0336210-mRNA-1
chr2_4	exon	55535707	55535788	55535707	ID=Msa0336210-mRNA-1:exon:16842;Parent=Msa0336210-mRNA-1
chr2_4	exon	55536880	55536929	55536880	ID=Msa0336210-mRNA-1:exon:16841;Parent=Msa0336210-mRNA-1
chr2_4	exon	55537024	55537066	55537024	ID=Msa0336210-mRNA-1:exon:16840;Parent=Msa0336210-mRNA-1
chr2_4	exon	55537162	55537242	55537162	ID=Msa0336210-mRNA-1:exon:16839;Parent=Msa0336210-mRNA-1
chr2_4	exon	55537333	55537378	55537333	ID=Msa0336210-mRNA-1:exon:16838;Parent=Msa0336210-mRNA-1
chr2_4	exon	55537491	55537537	55537491	ID=Msa0336210-mRNA-1:exon:16837;Parent=Msa0336210-mRNA-1
chr2_4	exon	55538446	55538511	55538446	ID=Msa0336210-mRNA-1:exon:16836;Parent=Msa0336210-mRNA-1
chr2_4	exon	55538812	55538878	55538812	ID=Msa0336210-mRNA-1:exon:16835;Parent=Msa0336210-mRNA-1
chr2_4	exon	55538976	55539454	55538976	ID=Msa0336210-mRNA-1:exon:16834;Parent=Msa0336210-mRNA-1
chr2_4	five_prime_UTR	55539074	55539454	55539074	ID=Msa0336210-mRNA-1:five_prime_utr;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55538976	55539073	55538976	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55538812	55538878	55538812	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55538446	55538511	55538446	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55537491	55537537	55537491	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55537333	55537378	55537333	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55537162	55537242	55537162	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55537024	55537066	55537024	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55536880	55536929	55536880	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55535707	55535788	55535707	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55535584	55535633	55535584	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55534208	55534281	55534208	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55533798	55533849	55533798	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55533054	55533704	55533054	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	CDS	55531370	55532965	55531370	ID=Msa0336210-mRNA-1:cds;Parent=Msa0336210-mRNA-1
chr2_4	three_prime_UTR	55531086	55531369	55531086	ID=Msa0336210-mRNA-1:three_prime_utr;Parent=Msa0336210-mRNA-1

Gene Sequence

>Msa0336210

ATGATGTCTAAGATGGATCAGTATGAGATCATGGAGCAGATCGGTCGCGGTGCATTTGGTGCTGCAATTCTCGTTAATCACAAGGTGGAGAAGAAACGATATGTGTTGAAGAAAATTAGGCTTGCAAGGCAAACGGAACGGTGTAGAAGATCTGCTCATCAAGAGATGGCTCTAATTGCGAGAATTAAGCATCCTTACATTGTTGAATTCAAGGAAGCTTGGGTTGAGAAGGGTTGCTATGTTTGCATTGTTACTGGATACTGTGAAGGTGGAGACATAGCAGCATTGATGAAAAAATCAAATGGGGCTTACTTTCCTGAAGAGAAACTCTGCAAATGGGTTACTCAATTACTGTTGGCAGTAGAATACCTGCATTCAAATTTTGTTTTGCACCGTGACCTAAAGTGTTCTAACATCTTTCTTACCAAGGATCATGATGTTCGCTTAGGGGATTTTGGGCTTGCTAAGACACTAAAAGCAGACGATTTAACTTCTTCGGTTGTAGGAACTCCCAACTATATGTGCCCTGAACTGCTTACAGATATTCCTTATGGATTCAAATCTGATATTTGGTCCCTAGGGTGTTGTATATATGAGATGGCTGCTCACCGCCCTGCTTTCAAAGCTTTTGATTTGGATGGATTGATAAGCAAGATCAACCGTTCCTCTATTGGTACTTTGCCTCCCTGTTATTCTCCATCCTTGAAAACACTGATAAAAGGCATGTTGAGGAAAAACCCCGAGCATCGTCCAACAGCATCCGATATCTTAAAGCACCCTTATTTGCAGCCTTATGTTCATCAATATTGTTCATCTCTCTGTTCTCCATCAGCCTCTTCTCCTGAAAAGCCAATTTTTGCTGTTCATGTTCCTCGAAAAACCATGGCAGAAAGTGAAAAAAGTAATAGTTCGTGTAGTGAAAAAGGTGGTTTGATGTCAAATAAGAGAAACGTTTCAAAAGCAGCACCAAAGGGTGATGACAAAATCACTGAGGTAGACCTGTCATCGATTGGTGAGAAAATCACTGACCTTATCATGCCCGGTAGAGAAAGAAATAGCTCTAGCAATGTCAATGCCAAAACAGATAAACAGGAGGTTAGGAAGCAAACTCACCTTGAACACAAATCTAATGTTGGATCTAAGCAAGCAAGGATAGTTAAGAATGCAAAGATGGGACCAAAACATGAAAAGGTTAAAGAGATGAGTTCTCCAATGAGAGGGAGCCTGATGAAGGCTGGCTGTATATCAACACAGAAAATTAATACAGAGACCTTGTGTAAATTTCCGAAATCAGATTTCGGTGTTAATGGTTTGAAGCCTAATGTAGAGGTGCCAACTTTAGCACCTTCAAAAGCAACCCTTCATTCTGCAAAACGAATCCAGGGATCACATACTTCAAAACATCAGCAGTTACCGATGATAGAACCTACACCAAAAACTAAACCTCGACAGAAGGTGACACCACCATGTGGTCCTATCAAACAAGTAGTAGCAGAACCTGAAGTTTCTGCACAGCCAAAACAAAAGACTCCTCCAAGCCTACTTAAGCCACCCTCATTTCTGGGACGCAATAAGCAAGCCGGGCATGATATGCCAAATGCAGCAAATGATAAAGAAAGGTTAGATACAAATAAAATAGCCCAGGAGGCTAAGATGAGACATCATCGGTTGCCTAACGGTCATCTGCCACATGTTTCTAGAGAGCCTTTGAAGGCTTTTGAAAGTAGTACCAACGGAATGCAAACAACCAGCAACAATTCTGTTTCATCTTCAGTCTCTGTTCAAGGATTTGAACTTTTTGACTTTGCAAACACTTTTATTGACTTGAGTGAACCAACACCCCCTGAACATGGGAGTTTTAATCACACTGAAATTGTGGGATCACTTGCCAACAGTAGTGGACCTGATGAAAATATTTCTGGCGAATCCTCTCCGTTTACACCAACTTTTCAGAATACCATAACTCGTAATGAGAAAGTAAGTCCCAGGTTAACTCTGGATCACTCAGTGATGTATGATAGTTTTCTTATAAATCAAACAACTACATCTGATTCCGGTGAGTGGGATGTTCCGTCTGCCAAAGTCACACAAGAAATCAAAGACCTTCAAGATTTTTGTAAGGAAATGGCTTCAATCAATTCTCTCAAACATTCACTTTCCATTTCTGGAAAAAAGTCAGTTTGTGAAGTTAGTCCAGTAAGCTTGCCAAATAATAGGCCTCATAAAGTTAGCCTGCCAAAGCTTATGTGCATCTCAACTGGTGATGACAAATTCATGGTGAGGGAAAGAGTGTCCTCAGTGGCTGAAATTGCTCCTGTGAATATATCTACTAAAATTTCAAGCCAGAAAGTTTTACAAGAAGAGAAGGGAATGGTTTTACAGAATCTAGCAACAGAAAGACCAGCTTCTGGCCATCTTCCCCGAGCTCTCGATGATGTCATCCATGTTATACGCCATAGCAGTTACCGGGTAGGGAGTGATCTTCCAGTAATGGAATCAACTGAGAAGGGAGTTACAAACGTAGATGTTGGTACACTCATCAACGTTGTAAAGGATGATTTAGAAATGAGAAATGTGGGAACTCCTTTGTCCCTTAAATCTTCAAATTGCTCTGAAGTTAATAGCTTGAACTCAAATACTATTGATAATTTGGAAATCAGAAACTTGAGCTTCAAGTCAAGTATTTCTGAACATCCTGGCGTTGAAGAGCAAGATGCGAGAAACCCTGATTTCGCTAAATATAACAAATATAGCCTTCCTACAGCTGAAGAGGAAATGCCAGAAAAGGAATCATTGGATGTTAAATCTTCCAAACAGAGAGCGGATGCACTTGAAGGGCTGTTAGAATTGTCTGCAGATTTGCTGCAGCAAAATAGATTGGAGGAACTTGCAGTTGTTCTCAAGCCCTTTGGAAAAGGTAAGGTCTCCTCAAGGGAGACAGCTATTTGGTTGGCCAAGAGTCTAAAAGGATTGATGGTTGAGGAAAGTAGGGGACGTAGTTAG

Protein sequence

>Msa0336210

MMSKMDQYEIMEQIGRGAFGAAILVNHKVEKKRYVLKKIRLARQTERCRRSAHQEMALIARIKHPYIVEFKEAWVEKGCYVCIVTGYCEGGDIAALMKKSNGAYFPEEKLCKWVTQLLLAVEYLHSNFVLHRDLKCSNIFLTKDHDVRLGDFGLAKTLKADDLTSSVVGTPNYMCPELLTDIPYGFKSDIWSLGCCIYEMAAHRPAFKAFDLDGLISKINRSSIGTLPPCYSPSLKTLIKGMLRKNPEHRPTASDILKHPYLQPYVHQYCSSLCSPSASSPEKPIFAVHVPRKTMAESEKSNSSCSEKGGLMSNKRNVSKAAPKGDDKITEVDLSSIGEKITDLIMPGRERNSSSNVNAKTDKQEVRKQTHLEHKSNVGSKQARIVKNAKMGPKHEKVKEMSSPMRGSLMKAGCISTQKINTETLCKFPKSDFGVNGLKPNVEVPTLAPSKATLHSAKRIQGSHTSKHQQLPMIEPTPKTKPRQKVTPPCGPIKQVVAEPEVSAQPKQKTPPSLLKPPSFLGRNKQAGHDMPNAANDKERLDTNKIAQEAKMRHHRLPNGHLPHVSREPLKAFESSTNGMQTTSNNSVSSSVSVQGFELFDFANTFIDLSEPTPPEHGSFNHTEIVGSLANSSGPDENISGESSPFTPTFQNTITRNEKVSPRLTLDHSVMYDSFLINQTTTSDSGEWDVPSAKVTQEIKDLQDFCKEMASINSLKHSLSISGKKSVCEVSPVSLPNNRPHKVSLPKLMCISTGDDKFMVRERVSSVAEIAPVNISTKISSQKVLQEEKGMVLQNLATERPASGHLPRALDDVIHVIRHSSYRVGSDLPVMESTEKGVTNVDVGTLINVVKDDLEMRNVGTPLSLKSSNCSEVNSLNSNTIDNLEIRNLSFKSSISEHPGVEEQDARNPDFAKYNKYSLPTAEEEMPEKESLDVKSSKQRADALEGLLELSADLLQQNRLEELAVVLKPFGKGKVSSRETAIWLAKSLKGLMVEESRGRS*