AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0340010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340010 MtrunA17_Chr2g0328801 96.667 120 4 0 1 120 1 120 8.41e-81 232
Msa0340010 MtrunA17_Chr2g0294091 95.000 120 6 0 1 120 1 120 5.86e-70 205
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0340010 AT5G49540.1 68.142 113 36 0 8 120 2 114 2.27e-51 158
Msa0340010 AT5G49540.2 66.038 106 36 0 8 113 2 107 1.43e-45 143

Find 43 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAGTTGTTGCTGGTATTT+TGG 0.142064 2_4:-61309833 Msa0340010:CDS
CGAACATTCTTATCTATAAT+TGG 0.198495 2_4:-61309856 Msa0340010:CDS
TGAATTACCTTTCTGTTTAC+AGG 0.199731 2_4:-61310153 Msa0340010:five_prime_UTR
AATATAATGGCTGGACATTC+TGG 0.202188 2_4:-61310108 Msa0340010:exon
TGCAGTCGTTCGTGCTGTTT+TGG 0.269177 2_4:-61307664 Msa0340010:intron
CTCATGGCATTTACTTCACT+TGG 0.282833 2_4:-61309772 Msa0340010:CDS
ATGACTCAGGATAGAAGTTT+TGG 0.300932 2_4:-61306943 Msa0340010:three_prime_UTR
ATCAATTAGCACACGGTTCC+AGG 0.313302 2_4:+61309705 None:intergenic
TCCATTGCTTGATTTCTTCT+CGG 0.315232 2_4:+61310074 None:intergenic
TTGTGATATAGATTGCAATT+TGG 0.322723 2_4:-61306990 Msa0340010:three_prime_UTR
TCATGTGTAATTTAATATAG+TGG 0.329218 2_4:-61307084 Msa0340010:three_prime_UTR
AAGTTTACATTGTTTAAAAT+CGG 0.333615 2_4:+61307128 None:intergenic
GATATTGTTTCATACGGAAT+TGG 0.336798 2_4:+61306967 None:intergenic
TGGCTTTCTCGGTGGTCTAA+TGG 0.351497 2_4:-61309683 Msa0340010:intron
TCCACACATACTTTGATTCC+TGG 0.356999 2_4:-61309723 Msa0340010:CDS
GTGCTAATTGATGGCTTTCT+CGG 0.375720 2_4:-61309694 Msa0340010:CDS
TCAAATTAGACAGAATATAA+TGG 0.404061 2_4:-61310121 Msa0340010:exon
ACATGAAAATCATCTATTAC+AGG 0.411097 2_4:-61310008 Msa0340010:intron
ATAATTGGCGGAGTTGTTGC+TGG 0.426188 2_4:-61309841 Msa0340010:CDS
GTCATTGATATTGTTTCATA+CGG 0.448634 2_4:+61306961 None:intergenic
TGGAACCGTGTGCTAATTGA+TGG 0.451359 2_4:-61309703 Msa0340010:CDS
AGTTTACATTGTTTAAAATC+GGG 0.453445 2_4:+61307129 None:intergenic
ATATAATGGCTGGACATTCT+GGG 0.478026 2_4:-61310107 Msa0340010:exon
ATGGCTGGACATTCTGGGTC+AGG 0.478468 2_4:-61310102 Msa0340010:CDS
TTGGGATTTACAGGCTTGAA+AGG 0.504477 2_4:-61309814 Msa0340010:CDS
CATGTGTAATTTAATATAGT+GGG 0.504632 2_4:-61307083 Msa0340010:three_prime_UTR
CAATTATAGATAAGAATGTT+CGG 0.514534 2_4:+61309857 None:intergenic
TGTCTTCTACGCACTTCTCA+TGG 0.518149 2_4:-61309788 Msa0340010:CDS
ATTAGACAGAATATAATGGC+TGG 0.519991 2_4:-61310117 Msa0340010:exon
TCATGGCATTTACTTCACTT+GGG 0.520496 2_4:-61309771 Msa0340010:CDS
ATCTGTTCCTGTAAACAGAA+AGG 0.531381 2_4:+61310146 None:intergenic
GAAAACTATCTCATAAGTGA+AGG 0.539622 2_4:+61307160 None:intergenic
ACATTCTTATCTATAATTGG+CGG 0.544008 2_4:-61309853 Msa0340010:CDS
TGAAACAATATCAATGACTC+AGG 0.563332 2_4:-61306956 Msa0340010:three_prime_UTR
AAAACTATCTCATAAGTGAA+GGG 0.593124 2_4:+61307161 None:intergenic
TCCGAGAAGAAATCAAGCAA+TGG 0.610929 2_4:-61310075 Msa0340010:CDS
TCCAGGAATCAAAGTATGTG+TGG 0.614397 2_4:+61309722 None:intergenic
GTGGGAAGCACTCAATGTCG+AGG 0.615276 2_4:-61307065 Msa0340010:three_prime_UTR
TCAATGTCGAGGGCTTGTTG+TGG 0.619681 2_4:-61307054 Msa0340010:three_prime_UTR
CTAATTGATGGCTTTCTCGG+TGG 0.637534 2_4:-61309691 Msa0340010:CDS
TGGGAAGCACTCAATGTCGA+GGG 0.640688 2_4:-61307064 Msa0340010:three_prime_UTR
GAAAGCCATCAATTAGCACA+CGG 0.703061 2_4:+61309698 None:intergenic
AAAACATTTGCACATCCCAG+CGG 0.738345 2_4:+61307240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAATTTTACATATAAATA+AGG + chr2_4:61308146-61308165 None:intergenic 10.0%
!!! CCATAATTATTTTAATATAT+CGG - chr2_4:61309192-61309211 Msa0340010:intron 10.0%
!! AAAATTTCAATATGATATAA+AGG + chr2_4:61309224-61309243 None:intergenic 10.0%
!! ATTATCAAATATGTTAAAAA+TGG - chr2_4:61309499-61309518 Msa0340010:intron 10.0%
!! ATAAATATGAAAAGAAATTT+GGG - chr2_4:61309558-61309577 Msa0340010:intron 10.0%
!!! TCTAAATTTTATTTTCTTTG+TGG + chr2_4:61307571-61307590 None:intergenic 15.0%
!!! AAAATAAAATTTAGAATGTC+AGG - chr2_4:61307576-61307595 Msa0340010:intron 15.0%
!!! AAATAAAATTTAGAATGTCA+GGG - chr2_4:61307577-61307596 Msa0340010:intron 15.0%
!! TCTATTAAAAAATATTGACA+AGG + chr2_4:61307797-61307816 None:intergenic 15.0%
!! TTAGTATGATTAATAACTAA+AGG + chr2_4:61308168-61308187 None:intergenic 15.0%
!! TATATAATTGCTGAATAAAT+GGG + chr2_4:61308670-61308689 None:intergenic 15.0%
!! ATATATAATTGCTGAATAAA+TGG + chr2_4:61308671-61308690 None:intergenic 15.0%
!!! TTTCTTTTTATTTCCTAAAA+GGG + chr2_4:61309000-61309019 None:intergenic 15.0%
!! ATATTAAAATAATTATGGAC+CGG + chr2_4:61309190-61309209 None:intergenic 15.0%
!! CCGATATATTAAAATAATTA+TGG + chr2_4:61309195-61309214 None:intergenic 15.0%
!!! TTATTTTAATATATCGGATA+AGG - chr2_4:61309198-61309217 Msa0340010:intron 15.0%
!!! TTTTTCATAATTGTATCAAA+AGG - chr2_4:61309529-61309548 Msa0340010:intron 15.0%
!! CATAAATATGAAAAGAAATT+TGG - chr2_4:61309557-61309576 Msa0340010:intron 15.0%
!!! AAGTTTACATTGTTTAAAAT+CGG + chr2_4:61309962-61309981 None:intergenic 15.0%
!! TCAAATTAGACAGAATATAA+TGG - chr2_4:61306966-61306985 Msa0340010:three_prime_UTR 20.0%
!! AAGTAAACATAGTATTAAGA+AGG + chr2_4:61307120-61307139 None:intergenic 20.0%
!! CAATTATAGATAAGAATGTT+CGG + chr2_4:61307233-61307252 None:intergenic 20.0%
!!! AGATAGAGAATATTTTCAAT+TGG + chr2_4:61308405-61308424 None:intergenic 20.0%
!!! AGATTTTCTAATGTTCTTAA+AGG - chr2_4:61308890-61308909 Msa0340010:intron 20.0%
!!! TTCTTTTTATTTCCTAAAAG+GGG + chr2_4:61308999-61309018 None:intergenic 20.0%
!!! CTTTCTTTTTATTTCCTAAA+AGG + chr2_4:61309001-61309020 None:intergenic 20.0%
!! AGATCATAATAATGTATATG+TGG - chr2_4:61309313-61309332 Msa0340010:intron 20.0%
!! GATCATAATAATGTATATGT+GGG - chr2_4:61309314-61309333 Msa0340010:intron 20.0%
!! ATCATAATAATGTATATGTG+GGG - chr2_4:61309315-61309334 Msa0340010:intron 20.0%
!! ATTCAAGCATAAAATGTATA+GGG + chr2_4:61309361-61309380 None:intergenic 20.0%
!!! AGTTTACATTGTTTAAAATC+GGG + chr2_4:61309961-61309980 None:intergenic 20.0%
!! TCATGTGTAATTTAATATAG+TGG - chr2_4:61310003-61310022 Msa0340010:intron 20.0%
!! CATGTGTAATTTAATATAGT+GGG - chr2_4:61310004-61310023 Msa0340010:intron 20.0%
!! ATTCAATGACTTTTACTGAA+AGG + chr2_4:61306919-61306938 None:intergenic 25.0%
! ACATGAAAATCATCTATTAC+AGG - chr2_4:61307079-61307098 Msa0340010:three_prime_UTR 25.0%
! AAAATCATCTATTACAGGTT+TGG - chr2_4:61307084-61307103 Msa0340010:three_prime_UTR 25.0%
! CGAACATTCTTATCTATAAT+TGG - chr2_4:61307231-61307250 Msa0340010:three_prime_UTR 25.0%
! ACATTCTTATCTATAATTGG+CGG - chr2_4:61307234-61307253 Msa0340010:three_prime_UTR 25.0%
!! TTAGTTAATAAGCTCTAGTT+TGG + chr2_4:61307639-61307658 None:intergenic 25.0%
! GTATATTCTTTATGCCATAA+AGG - chr2_4:61307693-61307712 Msa0340010:intron 25.0%
! ATTCAAACGAAAAACATCTA+AGG + chr2_4:61307864-61307883 None:intergenic 25.0%
!! TAGAGTATCAGGAAATATTA+TGG + chr2_4:61307939-61307958 None:intergenic 25.0%
!! TGCTGTATTTTCTAATTCTA+GGG - chr2_4:61308084-61308103 Msa0340010:intron 25.0%
! TAAATAAGGTCAATAACAGT+TGG + chr2_4:61308132-61308151 None:intergenic 25.0%
! TAGTACTAGATGTTAATTGT+AGG + chr2_4:61308454-61308473 None:intergenic 25.0%
! AAGGAATTTAATATTCATGG+AGG + chr2_4:61308542-61308561 None:intergenic 25.0%
! CACAAGGAATTTAATATTCA+TGG + chr2_4:61308545-61308564 None:intergenic 25.0%
! CTAGGTAAGTAATAATTATC+AGG + chr2_4:61308607-61308626 None:intergenic 25.0%
!!! AATAAATGGGTTAACAAGTA+TGG + chr2_4:61308657-61308676 None:intergenic 25.0%
!! TTTCAAAACATGTATTTCTG+TGG - chr2_4:61308718-61308737 Msa0340010:intron 25.0%
!! TTTCAAAACATGTATTTCTG+TGG - chr2_4:61308718-61308737 Msa0340010:intron 25.0%
!!! TCTTTTTATTTCCTAAAAGG+GGG + chr2_4:61308998-61309017 None:intergenic 25.0%
!!! CAACTTGCTATTCATATTAT+TGG - chr2_4:61309024-61309043 Msa0340010:intron 25.0%
! TCATAATAATGTATATGTGG+GGG - chr2_4:61309316-61309335 Msa0340010:intron 25.0%
! CATTCAAGCATAAAATGTAT+AGG + chr2_4:61309362-61309381 None:intergenic 25.0%
! AAGTATTATTGACAAAGAGA+AGG + chr2_4:61309455-61309474 None:intergenic 25.0%
! TTCTCTTTGTCAATAATACT+TGG - chr2_4:61309454-61309473 Msa0340010:intron 25.0%
! ATATGAAAAGAAATTTGGGT+TGG - chr2_4:61309562-61309581 Msa0340010:intron 25.0%
! TTGAAATGTTATGAATACAC+CGG - chr2_4:61309627-61309646 Msa0340010:intron 25.0%
! AAAACTATCTCATAAGTGAA+GGG + chr2_4:61309929-61309948 None:intergenic 25.0%
! TTGTGATATAGATTGCAATT+TGG - chr2_4:61310097-61310116 Msa0340010:CDS 25.0%
! GTCATTGATATTGTTTCATA+CGG + chr2_4:61310129-61310148 None:intergenic 25.0%
TGAATTACCTTTCTGTTTAC+AGG - chr2_4:61306934-61306953 Msa0340010:three_prime_UTR 30.0%
ATTAGACAGAATATAATGGC+TGG - chr2_4:61306970-61306989 Msa0340010:three_prime_UTR 30.0%
CATGAATGAAGAAATCAGTT+TGG - chr2_4:61307491-61307510 Msa0340010:intron 30.0%
GAAATCAGTTTGGATAAGAT+AGG - chr2_4:61307501-61307520 Msa0340010:intron 30.0%
GAGTAAACACACATACAAAT+AGG - chr2_4:61307531-61307550 Msa0340010:intron 30.0%
AAAAGACACATCAACAGTTT+AGG - chr2_4:61307608-61307627 Msa0340010:intron 30.0%
AAAGACACATCAACAGTTTA+GGG - chr2_4:61307609-61307628 Msa0340010:intron 30.0%
TATTAACTAATAGAATCCCC+TGG - chr2_4:61307649-61307668 Msa0340010:intron 30.0%
! CCTTCATAAGTTCAAACTTT+TGG + chr2_4:61307963-61307982 None:intergenic 30.0%
CCAAAAGTTTGAACTTATGA+AGG - chr2_4:61307960-61307979 Msa0340010:intron 30.0%
! GTGCTGTATTTTCTAATTCT+AGG - chr2_4:61308083-61308102 Msa0340010:intron 30.0%
!! CTAGTAACCTTTGAAATCAT+CGG - chr2_4:61308217-61308236 Msa0340010:intron 30.0%
GAAAAAATGATTTCGACTCT+TGG + chr2_4:61308335-61308354 None:intergenic 30.0%
!!! ATCATTTTTTCCAGGTTACA+TGG - chr2_4:61308344-61308363 Msa0340010:intron 30.0%
! GTAGGAACTTTAATTTCTCT+AGG + chr2_4:61308436-61308455 None:intergenic 30.0%
GGAGAAAGACAAAATAATCA+AGG - chr2_4:61308511-61308530 Msa0340010:intron 30.0%
TCTAGGTAAATCTTAGGTTA+GGG + chr2_4:61308570-61308589 None:intergenic 30.0%
TTCTAGGTAAATCTTAGGTT+AGG + chr2_4:61308571-61308590 None:intergenic 30.0%
AATTATCAGGTTAGACACTT+GGG + chr2_4:61308594-61308613 None:intergenic 30.0%
TAATTATCAGGTTAGACACT+TGG + chr2_4:61308595-61308614 None:intergenic 30.0%
TTAACAAGTATGGTTTAGCT+AGG + chr2_4:61308647-61308666 None:intergenic 30.0%
!! CTTTTTATTTCCTAAAAGGG+GGG + chr2_4:61308997-61309016 None:intergenic 30.0%
TATTATTGGCATCCCTTATA+TGG - chr2_4:61309038-61309057 Msa0340010:intron 30.0%
AACATCAGAAGTCCATATAA+GGG + chr2_4:61309053-61309072 None:intergenic 30.0%
AAACATCAGAAGTCCATATA+AGG + chr2_4:61309054-61309073 None:intergenic 30.0%
CATAATAATGTATATGTGGG+GGG - chr2_4:61309317-61309336 Msa0340010:intron 30.0%
!! TTGTTAAGTTGAATGAGCAT+GGG + chr2_4:61309684-61309703 None:intergenic 30.0%
!! ATTGTTAAGTTGAATGAGCA+TGG + chr2_4:61309685-61309704 None:intergenic 30.0%
!!! GTTTTAGGTTGTGTTTTAGT+AGG + chr2_4:61309724-61309743 None:intergenic 30.0%
AAAACACAACCTAAAACAAC+CGG - chr2_4:61309727-61309746 Msa0340010:CDS 30.0%
! TGCAAATCTGTATTTTGTCA+AGG + chr2_4:61309803-61309822 None:intergenic 30.0%
GAAAACTATCTCATAAGTGA+AGG + chr2_4:61309930-61309949 None:intergenic 30.0%
GATATTGTTTCATACGGAAT+TGG + chr2_4:61310123-61310142 None:intergenic 30.0%
TGAAACAATATCAATGACTC+AGG - chr2_4:61310131-61310150 Msa0340010:five_prime_UTR 30.0%
ATCTGTTCCTGTAAACAGAA+AGG + chr2_4:61306944-61306963 None:intergenic 35.0%
AATATAATGGCTGGACATTC+TGG - chr2_4:61306979-61306998 Msa0340010:three_prime_UTR 35.0%
ATATAATGGCTGGACATTCT+GGG - chr2_4:61306980-61306999 Msa0340010:three_prime_UTR 35.0%
TCCATTGCTTGATTTCTTCT+CGG + chr2_4:61307016-61307035 None:intergenic 35.0%
TGACTGTTCAATGTTGTCTA+TGG - chr2_4:61307174-61307193 Msa0340010:three_prime_UTR 35.0%
TCATGGCATTTACTTCACTT+GGG - chr2_4:61307316-61307335 Msa0340010:intron 35.0%
CACTTAGAGAGACTATGAAA+AGG - chr2_4:61307830-61307849 Msa0340010:intron 35.0%
!! AAACTTTTGGCTAGAGTATC+AGG + chr2_4:61307950-61307969 None:intergenic 35.0%
AAAATGCTTCAACTGTTGAG+AGG - chr2_4:61307983-61308002 Msa0340010:intron 35.0%
CAAGCAGCTAATTACATTTG+GGG + chr2_4:61308046-61308065 None:intergenic 35.0%
GCAAGCAGCTAATTACATTT+GGG + chr2_4:61308047-61308066 None:intergenic 35.0%
!! CAGCACGTTTATTTTCACAA+TGG + chr2_4:61308069-61308088 None:intergenic 35.0%
AAGGTTACTAGTAGATTCAC+AGG + chr2_4:61308208-61308227 None:intergenic 35.0%
AGAAAAATTTCTGCCAGAGA+AGG + chr2_4:61308246-61308265 None:intergenic 35.0%
! GGCAGAAATTTTTCTCTGTT+TGG - chr2_4:61308251-61308270 Msa0340010:intron 35.0%
!!! GAGTCGAAATCATTTTTTCC+AGG - chr2_4:61308336-61308355 Msa0340010:intron 35.0%
AGATCATAAGAGACTGATAC+TGG - chr2_4:61308489-61308508 Msa0340010:intron 35.0%
GATCATAAGAGACTGATACT+GGG - chr2_4:61308490-61308509 Msa0340010:intron 35.0%
!! TTGGGTTCTAGGTAAATCTT+AGG + chr2_4:61308576-61308595 None:intergenic 35.0%
!!! GTGATCACTTTTGCTATTAC+TGG - chr2_4:61308765-61308784 Msa0340010:intron 35.0%
ACTTCTGATGTTTGTTTGAG+CGG - chr2_4:61309061-61309080 Msa0340010:intron 35.0%
!! TTCCCTTAATTTGCAGAGAA+AGG + chr2_4:61309140-61309159 None:intergenic 35.0%
AGGGAAAAGAGAGATAGAAA+AGG - chr2_4:61309154-61309173 Msa0340010:intron 35.0%
GGGAAAAGAGAGATAGAAAA+GGG - chr2_4:61309155-61309174 Msa0340010:intron 35.0%
GGAAAAGAGAGATAGAAAAG+GGG - chr2_4:61309156-61309175 Msa0340010:intron 35.0%
AAATGTATAGGGAGCAAAAG+AGG + chr2_4:61309350-61309369 None:intergenic 35.0%
!! AAAAGAAATTTGGGTTGGTG+AGG - chr2_4:61309567-61309586 Msa0340010:intron 35.0%
AAATGTTATGAATACACCGG+TGG - chr2_4:61309630-61309649 Msa0340010:intron 35.0%
AGAAAATCTGAAAGTTCCAC+CGG + chr2_4:61309649-61309668 None:intergenic 35.0%
AGATAGATATGCAGGAATAC+CGG + chr2_4:61309749-61309768 None:intergenic 35.0%
TAAGCAGAAGATAGATATGC+AGG + chr2_4:61309757-61309776 None:intergenic 35.0%
!!! AGACAGTTTTTTCTCCATTG+CGG - chr2_4:61309876-61309895 Msa0340010:intron 35.0%
! TTCATTTTGAATAGCCGCAA+TGG + chr2_4:61309893-61309912 None:intergenic 35.0%
! ATGACTCAGGATAGAAGTTT+TGG - chr2_4:61310144-61310163 Msa0340010:five_prime_UTR 35.0%
TCCGAGAAGAAATCAAGCAA+TGG - chr2_4:61307012-61307031 Msa0340010:three_prime_UTR 40.0%
! GGAGTTGTTGCTGGTATTTT+GGG - chr2_4:61307255-61307274 Msa0340010:exon 40.0%
!!! GCTGGTATTTTGGGATTTAC+AGG - chr2_4:61307264-61307283 Msa0340010:CDS 40.0%
TTGGGATTTACAGGCTTGAA+AGG - chr2_4:61307273-61307292 Msa0340010:CDS 40.0%
CTCATGGCATTTACTTCACT+TGG - chr2_4:61307315-61307334 Msa0340010:intron 40.0%
TCCACACATACTTTGATTCC+TGG - chr2_4:61307364-61307383 Msa0340010:intron 40.0%
TCCAGGAATCAAAGTATGTG+TGG + chr2_4:61307368-61307387 None:intergenic 40.0%
GAAAGCCATCAATTAGCACA+CGG + chr2_4:61307392-61307411 None:intergenic 40.0%
! GTGCTAATTGATGGCTTTCT+CGG - chr2_4:61307393-61307412 Msa0340010:intron 40.0%
ACCTCATTCATGTTGTTCCA+GGG + chr2_4:61307669-61307688 None:intergenic 40.0%
TTATGCCATAAAGGATGCAG+TGG - chr2_4:61307702-61307721 Msa0340010:intron 40.0%
! TATCTCGTGGAATCTTTTGC+TGG + chr2_4:61307899-61307918 None:intergenic 40.0%
GAACTTTGCAAACTATCTCG+TGG + chr2_4:61307912-61307931 None:intergenic 40.0%
TAGTCACCAGACACACTAAA+TGG - chr2_4:61308016-61308035 Msa0340010:intron 40.0%
! CAGCTAATTACATTTGGGGT+AGG + chr2_4:61308042-61308061 None:intergenic 40.0%
GGCAAGCAGCTAATTACATT+TGG + chr2_4:61308048-61308067 None:intergenic 40.0%
AAGGTCACCGATGATTTCAA+AGG + chr2_4:61308227-61308246 None:intergenic 40.0%
CTCTGTTTGGACTCAGAAAA+TGG - chr2_4:61308264-61308283 Msa0340010:intron 40.0%
TGTTCATTTGCCATGTAACC+TGG + chr2_4:61308357-61308376 None:intergenic 40.0%
AGGTTACATGGCAAATGAAC+AGG - chr2_4:61308356-61308375 Msa0340010:intron 40.0%
GGTTACATGGCAAATGAACA+GGG - chr2_4:61308357-61308376 Msa0340010:intron 40.0%
! ATTTACTGACTGGGAGAGAA+CGG + chr2_4:61309112-61309131 None:intergenic 40.0%
TCTCTCCCAGTCAGTAAATT+AGG - chr2_4:61309113-61309132 Msa0340010:intron 40.0%
CTCTCCCAGTCAGTAAATTA+GGG - chr2_4:61309114-61309133 Msa0340010:intron 40.0%
! AAGGCCCTAATTTACTGACT+GGG + chr2_4:61309121-61309140 None:intergenic 40.0%
AAAGGCCCTAATTTACTGAC+TGG + chr2_4:61309122-61309141 None:intergenic 40.0%
GGCCTTTCTCTGCAAATTAA+GGG - chr2_4:61309135-61309154 Msa0340010:intron 40.0%
AAAGAGAGATAGAAAAGGGG+AGG - chr2_4:61309159-61309178 Msa0340010:intron 40.0%
TAGAAAAGGGGAGGTTTATC+CGG - chr2_4:61309168-61309187 Msa0340010:intron 40.0%
ATTGCCTTGCTGATCTTATC+TGG - chr2_4:61309271-61309290 Msa0340010:intron 40.0%
GACTGCATGGGATCAAAAAA+AGG + chr2_4:61309410-61309429 None:intergenic 40.0%
!! AATTTGGGTTGGTGAGGATT+TGG - chr2_4:61309573-61309592 Msa0340010:intron 40.0%
!! ATTTGGGTTGGTGAGGATTT+GGG - chr2_4:61309574-61309593 Msa0340010:intron 40.0%
AAGTTGAATGAGCATGGGAA+TGG + chr2_4:61309679-61309698 None:intergenic 40.0%
!!! TGTGTTTTAGTAGGATGCTC+TGG + chr2_4:61309715-61309734 None:intergenic 40.0%
!!! TGCTCATATATTTTGACCGC+TGG - chr2_4:61309831-61309850 Msa0340010:CDS 40.0%
!!! GCTCATATATTTTGACCGCT+GGG - chr2_4:61309832-61309851 Msa0340010:CDS 40.0%
AAAACATTTGCACATCCCAG+CGG + chr2_4:61309850-61309869 None:intergenic 40.0%
ATAATTGGCGGAGTTGTTGC+TGG - chr2_4:61307246-61307265 Msa0340010:exon 45.0%
! CGGAGTTGTTGCTGGTATTT+TGG - chr2_4:61307254-61307273 Msa0340010:exon 45.0%
TGTCTTCTACGCACTTCTCA+TGG - chr2_4:61307299-61307318 Msa0340010:intron 45.0%
ATCAATTAGCACACGGTTCC+AGG + chr2_4:61307385-61307404 None:intergenic 45.0%
TGGAACCGTGTGCTAATTGA+TGG - chr2_4:61307384-61307403 Msa0340010:intron 45.0%
! CTAATTGATGGCTTTCTCGG+TGG - chr2_4:61307396-61307415 Msa0340010:intron 45.0%
TTTCTCGGTGGTCTAATGGT+AGG - chr2_4:61307408-61307427 Msa0340010:intron 45.0%
CCTCATTCATGTTGTTCCAG+GGG + chr2_4:61307668-61307687 None:intergenic 45.0%
GACCTCATTCATGTTGTTCC+AGG + chr2_4:61307670-61307689 None:intergenic 45.0%
ATTCACAGGCTTCGACATAG+TGG + chr2_4:61308194-61308213 None:intergenic 45.0%
AAATCATCGGTGACCTTCTC+TGG - chr2_4:61308230-61308249 Msa0340010:intron 45.0%
ATCTTAGGTTAGGGTGCACA+AGG + chr2_4:61308561-61308580 None:intergenic 45.0%
AGGTTAGACACTTGGGTTCT+AGG + chr2_4:61308587-61308606 None:intergenic 45.0%
TTAGCTAGGTGACTAAGCGT+GGG + chr2_4:61308633-61308652 None:intergenic 45.0%
TTTAGCTAGGTGACTAAGCG+TGG + chr2_4:61308634-61308653 None:intergenic 45.0%
! ACATAGCTTTCCCCCCTTTT+AGG - chr2_4:61308984-61309003 Msa0340010:intron 45.0%
! TTTACTGACTGGGAGAGAAC+GGG + chr2_4:61309111-61309130 None:intergenic 45.0%
GGGCCTTTCTCTGCAAATTA+AGG - chr2_4:61309134-61309153 Msa0340010:intron 45.0%
CTTGCCAGATAAGATCAGCA+AGG + chr2_4:61309278-61309297 None:intergenic 45.0%
!! GCAGGAATACCGGTTGTTTT+AGG + chr2_4:61309739-61309758 None:intergenic 45.0%
TGGCTTTCTCGGTGGTCTAA+TGG - chr2_4:61307404-61307423 Msa0340010:intron 50.0%
CCCCTGGAACAACATGAATG+AGG - chr2_4:61307665-61307684 Msa0340010:intron 50.0%
CTTCGCCACTGCATCCTTTA+TGG + chr2_4:61307710-61307729 None:intergenic 50.0%
GGTAGGCCATTTAGTGTGTC+TGG + chr2_4:61308025-61308044 None:intergenic 50.0%
GTGACTAAGCGTGGGAATCT+AGG + chr2_4:61308625-61308644 None:intergenic 50.0%
AACAGCACGAACGACTGCAT+GGG + chr2_4:61309422-61309441 None:intergenic 50.0%
AAACAGCACGAACGACTGCA+TGG + chr2_4:61309423-61309442 None:intergenic 50.0%
!!! TGCAGTCGTTCGTGCTGTTT+TGG - chr2_4:61309423-61309442 Msa0340010:intron 50.0%
!! TGGGAAGCACTCAATGTCGA+GGG - chr2_4:61310023-61310042 Msa0340010:CDS 50.0%
!! TCAATGTCGAGGGCTTGTTG+TGG - chr2_4:61310033-61310052 Msa0340010:CDS 50.0%
ATGGCTGGACATTCTGGGTC+AGG - chr2_4:61306985-61307004 Msa0340010:three_prime_UTR 55.0%
!! GTGGGAAGCACTCAATGTCG+AGG - chr2_4:61310022-61310041 Msa0340010:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 61306906 61310203 61306906 ID=Msa0340010;Name=Msa0340010
chr2_4 mRNA 61306906 61310203 61306906 ID=Msa0340010-mRNA-1;Parent=Msa0340010;Name=Msa0340010-mRNA-1;_AED=0.07;_eAED=0.07;_QI=79|1|1|1|0.33|0.5|4|357|120
chr2_4 exon 61306906 61307293 61306906 ID=Msa0340010-mRNA-1:exon:19242;Parent=Msa0340010-mRNA-1
chr2_4 exon 61307662 61307681 61307662 ID=Msa0340010-mRNA-1:exon:19241;Parent=Msa0340010-mRNA-1
chr2_4 exon 61309684 61309879 61309684 ID=Msa0340010-mRNA-1:exon:19240;Parent=Msa0340010-mRNA-1
chr2_4 exon 61310009 61310203 61310009 ID=Msa0340010-mRNA-1:exon:19239;Parent=Msa0340010-mRNA-1
chr2_4 five_prime_UTR 61310125 61310203 61310125 ID=Msa0340010-mRNA-1:five_prime_utr;Parent=Msa0340010-mRNA-1
chr2_4 CDS 61310009 61310124 61310009 ID=Msa0340010-mRNA-1:cds;Parent=Msa0340010-mRNA-1
chr2_4 CDS 61309684 61309879 61309684 ID=Msa0340010-mRNA-1:cds;Parent=Msa0340010-mRNA-1
chr2_4 CDS 61307662 61307681 61307662 ID=Msa0340010-mRNA-1:cds;Parent=Msa0340010-mRNA-1
chr2_4 CDS 61307263 61307293 61307263 ID=Msa0340010-mRNA-1:cds;Parent=Msa0340010-mRNA-1
chr2_4 three_prime_UTR 61306906 61307262 61306906 ID=Msa0340010-mRNA-1:three_prime_utr;Parent=Msa0340010-mRNA-1
Gene Sequence

>Msa0340010

ATGGCTGGACATTCTGGGTCAGGTTCATCCGAGAAGAAATCAAGCAATGGACTGAATGAGTTGCTGACTTTTAATGCTGAGAATATGCAAAGCAACATGAAAATCATCTATTACAGCCGAACATTCTTATCTATAATTGGCGGAGTTGTTGCTGGTATTTTGGGATTTACAGGCTTGAAAGGATTTGTCTTCTACGCACTTCTCATGGCATTTACTTCACTTGGGCTCATAGTCAAAGCAAAATTTTCAATCCACACATACTTTGATTCCTGGAACCGTGTGCTAATTGATGGCTTTCTCGGTGGTCTAATGTCGTTCGTGCTGTTTTGGACATTTGCATATGACATTGCTCATATATTTTGA
Protein sequence

>Msa0340010

MAGHSGSGSSEKKSSNGLNELLTFNAENMQSNMKIIYYSRTFLSIIGGVVAGILGFTGLKGFVFYALLMAFTSLGLIVKAKFSIHTYFDSWNRVLIDGFLGGLMSFVLFWTFAYDIAHIF*