Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0340390 | XP_013445174.1 | 55.556 | 72 | 31 | 1 | 5 | 76 | 879 | 949 | 1.48e-17 | 86.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0340390 | Msa0340410 | 0.809300 | 2.025185e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0340390 | MtrunA17_Chr8g0354711 | 55.556 | 72 | 31 | 1 | 5 | 76 | 879 | 949 | 1.36e-21 | 86.7 |
| Msa0340390 | MtrunA17_Chr8g0354701 | 60.000 | 55 | 21 | 1 | 22 | 76 | 901 | 954 | 1.08e-15 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCAGGAATCTTTGTTCC+TGG | 0.369345 | 2_4:+61763454 | None:intergenic |
| GTGAAATACATGAACCTTGC+AGG | 0.385375 | 2_4:-61764021 | Msa0340390:CDS |
| GCAGATAAATGATCCATGTT+TGG | 0.396670 | 2_4:+61763938 | None:intergenic |
| TTCCCAGTGTGCAAGGCTGC+AGG | 0.401199 | 2_4:-61763894 | Msa0340390:CDS |
| AGCCTGCAGCCTTGCACACT+GGG | 0.427887 | 2_4:+61763892 | None:intergenic |
| TGCAAGGTTCATGTATTTCA+CGG | 0.428060 | 2_4:+61764023 | None:intergenic |
| GAACAGTGTGCAAATCAATC+AGG | 0.475964 | 2_4:+61763439 | None:intergenic |
| AAGCCTGCAGCCTTGCACAC+TGG | 0.479619 | 2_4:+61763891 | None:intergenic |
| TTATACATTCGAGAAATTGA+AGG | 0.528538 | 2_4:-61763968 | Msa0340390:CDS |
| TTATAGTGTATCAGGCAGAT+TGG | 0.531432 | 2_4:-61763762 | Msa0340390:intron |
| TGACAGGAAATGATTGAAGC+TGG | 0.542559 | 2_4:-61763496 | Msa0340390:intron |
| TATTGACTTCCCAGTGTGCA+AGG | 0.543517 | 2_4:-61763901 | Msa0340390:CDS |
| TGAAGGAGTTCTGCCAAACA+TGG | 0.547371 | 2_4:-61763951 | Msa0340390:CDS |
| TCAAGCAACATTCTCCTGCA+AGG | 0.607797 | 2_4:+61764007 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTATACATTCGAGAAATTGA+AGG | - | chr2_4:61763516-61763535 | Msa0340390:intron | 25.0% |
| ! | AAATAAACAGAGATCAAAAG+AGG | + | chr2_4:61763638-61763657 | None:intergenic | 25.0% |
| ! | AATTGATGTTATAGTGTATC+AGG | - | chr2_4:61763714-61763733 | Msa0340390:intron | 25.0% |
| ! | TTCTCTGATTTCATTAGTAT+CGG | - | chr2_4:61763887-61763906 | Msa0340390:intron | 25.0% |
| ! | GGAATTGATACATATTTGAT+AGG | + | chr2_4:61763915-61763934 | None:intergenic | 25.0% |
| ! | TTTCTAAATTTATGACTGAC+AGG | - | chr2_4:61763972-61763991 | Msa0340390:CDS | 25.0% |
| GAAACTTATAACAGTTACTG+TGG | + | chr2_4:61763827-61763846 | None:intergenic | 30.0% | |
| !!! | AACAAGATGTTTTATTTGCC+AGG | - | chr2_4:61764012-61764031 | Msa0340390:CDS | 30.0% |
| TGCAAGGTTCATGTATTTCA+CGG | + | chr2_4:61763464-61763483 | None:intergenic | 35.0% | |
| GCAGATAAATGATCCATGTT+TGG | + | chr2_4:61763549-61763568 | None:intergenic | 35.0% | |
| TAAACAGAGATCAAAAGAGG+AGG | + | chr2_4:61763635-61763654 | None:intergenic | 35.0% | |
| TTATAGTGTATCAGGCAGAT+TGG | - | chr2_4:61763722-61763741 | Msa0340390:intron | 35.0% | |
| ! | AGCAGCTTGATGCTATTTAA+AGG | - | chr2_4:61763789-61763808 | Msa0340390:intron | 35.0% |
| !!! | TAGCGTTTTGAGACATTGAT+AGG | + | chr2_4:61763936-61763955 | None:intergenic | 35.0% |
| GTGAAATACATGAACCTTGC+AGG | - | chr2_4:61763463-61763482 | Msa0340390:CDS | 40.0% | |
| ! | GTATCAGGCAGATTGGTTAA+TGG | - | chr2_4:61763729-61763748 | Msa0340390:intron | 40.0% |
| TGACAGGAAATGATTGAAGC+TGG | - | chr2_4:61763988-61764007 | Msa0340390:CDS | 40.0% | |
| CAATCAGGAATCTTTGTTCC+TGG | + | chr2_4:61764033-61764052 | None:intergenic | 40.0% | |
| TCAAGCAACATTCTCCTGCA+AGG | + | chr2_4:61763480-61763499 | None:intergenic | 45.0% | |
| TGAAGGAGTTCTGCCAAACA+TGG | - | chr2_4:61763533-61763552 | Msa0340390:intron | 45.0% | |
| !! | TATTGACTTCCCAGTGTGCA+AGG | - | chr2_4:61763583-61763602 | Msa0340390:intron | 45.0% |
| !! | TGCAGGCTTGTTAGTGAATC+AGG | - | chr2_4:61763607-61763626 | Msa0340390:intron | 45.0% |
| TTCCCAGTGTGCAAGGCTGC+AGG | - | chr2_4:61763590-61763609 | Msa0340390:intron | 60.0% | |
| AGCCTGCAGCCTTGCACACT+GGG | + | chr2_4:61763595-61763614 | None:intergenic | 60.0% | |
| AAGCCTGCAGCCTTGCACAC+TGG | + | chr2_4:61763596-61763615 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 61763453 | 61764053 | 61763453 | ID=Msa0340390;Name=Msa0340390 |
| chr2_4 | mRNA | 61763453 | 61764053 | 61763453 | ID=Msa0340390-mRNA-1;Parent=Msa0340390;Name=Msa0340390-mRNA-1;_AED=0.82;_eAED=0.82;_QI=0|0|0|0.33|1|1|3|0|76 |
| chr2_4 | exon | 61763891 | 61764053 | 61763891 | ID=Msa0340390-mRNA-1:exon:19538;Parent=Msa0340390-mRNA-1 |
| chr2_4 | exon | 61763763 | 61763770 | 61763763 | ID=Msa0340390-mRNA-1:exon:19537;Parent=Msa0340390-mRNA-1 |
| chr2_4 | exon | 61763453 | 61763512 | 61763453 | ID=Msa0340390-mRNA-1:exon:19536;Parent=Msa0340390-mRNA-1 |
| chr2_4 | CDS | 61763891 | 61764053 | 61763891 | ID=Msa0340390-mRNA-1:cds;Parent=Msa0340390-mRNA-1 |
| chr2_4 | CDS | 61763763 | 61763770 | 61763763 | ID=Msa0340390-mRNA-1:cds;Parent=Msa0340390-mRNA-1 |
| chr2_4 | CDS | 61763453 | 61763512 | 61763453 | ID=Msa0340390-mRNA-1:cds;Parent=Msa0340390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
